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Hydrogen sulfide protects retina from blue light-induced photodamage and degeneration via inhibiting ROS-mediated ER stress-CHOP apoptosis signal 硫化氢通过抑制ros介导的内质网应激- chop凋亡信号保护视网膜免受蓝光诱导的光损伤和变性
IF 3.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-04-28 DOI: 10.1080/13510002.2022.2069534
Sen Zhu, Xuan Li, B. Dang, Fen-Shiun Wu, K. Gou, Chunming Wang, Changjun Lin
ABSTRACT Background: Hydrogen sulfide (H2S) is a small reducing gas molecule with various biological functions such as anti-oxidative, anti-apoptotic and anti-inflammatory activities. In this study, we investigated the therapeutic effects of exogenous H2S in the experimental models of retinal photodamage in vivo and in vitro. Methods: Rats with open eyelids were pretreated with H2S (80~120 μmol/kg) for 10 days and then continuously exposed to blue light (435~445nm, 11.2W/m2) for 8 h to establish in vivo experimental model. ARPE-19 cells were pretreated with H2S and then exposed to blue light to establish in vitro experimental model. Results: In vivo experiments, H2S significantly ameliorated blue light-induced retinal oxidative stress, apoptosis and degeneration. Moreover, H2S inhibited the activation of blue light-induced endoplasmic reticulum (ER) stress CHOP apoptotic signaling. In vitro experiments, H2S improved blue light-induced oxidative stress and oxidative damage. H2S inhibited ROS-mediated activation of ER stress CHOP apoptotic signaling. H2S alleviated blue light-induced apoptosis and increases cell viability. The ER stress inhibitor 4-PBA alleviated blue light-induced apoptosis and increases cell viability. Conclusion: Taken together, these results indicate that H2S can inhibit ROS-mediated ER stress-CHOP apoptosis signal, thereby alleviating blue light-triggered retinal apoptosis and degeneration.
背景:硫化氢(H2S)是一种小型还原性气体分子,具有抗氧化、抗凋亡、抗炎等多种生物学功能。在本研究中,我们研究了外源性H2S对视网膜光损伤实验模型的体内和体外治疗作用。方法:用H2S (80~120 μmol/kg)预处理裸眼大鼠10 d,然后连续暴露于435~445nm、11.2W/m2的蓝光下8 h,建立体内实验模型。用H2S预处理ARPE-19细胞,然后蓝光照射建立体外实验模型。结果:在体内实验中,H2S可显著改善蓝光诱导的视网膜氧化应激、细胞凋亡和变性。H2S抑制蓝光诱导内质网(ER)应激CHOP凋亡信号的激活。体外实验中,H2S可改善蓝光诱导的氧化应激和氧化损伤。H2S抑制ros介导的内质网应激CHOP凋亡信号的激活。H2S可减轻蓝光诱导的细胞凋亡,提高细胞活力。内质网应激抑制剂4-PBA可减轻蓝光诱导的细胞凋亡,提高细胞活力。结论:综上所述,H2S可抑制ros介导的ER应激- chop凋亡信号,从而减轻蓝光引发的视网膜凋亡和变性。
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引用次数: 9
SOD2 rs4880 and GPX1 rs1050450 polymorphisms do not confer risk of COVID-19, but influence inflammation or coagulation parameters in Serbian cohort 在塞尔维亚队列中,SOD2 rs4880和GPX1 rs1050450多态性不会增加COVID-19的风险,但会影响炎症或凝血参数
IF 3.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-03-31 DOI: 10.1080/13510002.2022.2057707
Djurdja Jerotić, J. Ranin, Z. Bukumirić, Tatjana Djukic, V. Ćorić, A. Savić-Radojević, N. Todorović, M. Ašanin, M. Ercegovac, I. Milošević, M. Plješa-Ercegovac, G. Stevanović, M. Matić, Tatjana Simić
ABSTRACT Objectives: Due to the role of oxidative stress in the pathophysiology of COVID-19, it is biologically plausible that inter-individual differences in patients’ clinical manifestations might be affected by antioxidant genetic profile. The aim of our study was to assess the distribution of antioxidant genetic polymorphisms Nrf2 rs6721961, SOD2 rs4880, GPX1 rs1050450, GPX3 rs8177412, and GSTP1 (rs1695 and rs1138272) haplotype in COVID-19 patients and controls, with special emphasis on their association with laboratory biochemical parameters. Methods: The antioxidant genetic polymorphisms were assessed by appropriate PCR methods in 229 COVID-19 patients and 229 matched healthy individuals. Results: Among examined polymorphisms, only GSTP1 haplotype was associated with COVID-19 risk (p = 0.009). Polymorphisms of SOD2 and GPX1 influenced COVID-19 patients’ laboratory biochemical profile: SOD2*Val allele was associated with increased levels of fibrinogen (p = 0.040) and ferritin (p = 0.033), whereas GPX1*Leu allele was associated with D-dimmer (p = 0.009). Discussion: Our findings regarding the influence of SOD2 and GPX1 polymorphisms on inflammation and coagulation parameters might be of clinical importance. If confirmed in larger cohorts, these developments could provide a more personalized approach for better recognition of patients prone to thrombosis and those for the need of targeted antioxidant therapy.
摘要目的:由于氧化应激在COVID-19病理生理中的作用,从生物学上讲,患者临床表现的个体差异可能受到抗氧化基因谱的影响。本研究的目的是评估抗氧化遗传多态性Nrf2 rs6721961、SOD2 rs4880、GPX1 rs1050450、GPX3 rs8177412和GSTP1 (rs1695和rs1138272)单倍型在COVID-19患者和对照组中的分布,并特别强调它们与实验室生化参数的相关性。方法:采用相应的PCR方法对229例新冠肺炎患者和229例匹配的健康个体的抗氧化基因多态性进行检测。结果:在所检测的多态性中,只有GSTP1单倍型与COVID-19风险相关(p = 0.009)。SOD2和GPX1的多态性影响COVID-19患者的实验室生化特征:SOD2*Val等位基因与纤维蛋白原(p = 0.040)和铁蛋白(p = 0.033)水平升高相关,GPX1*Leu等位基因与d -二聚体(p = 0.009)相关。讨论:我们关于SOD2和GPX1多态性对炎症和凝血参数的影响的研究结果可能具有临床意义。如果在更大的队列中得到证实,这些进展可以为更好地识别血栓易感性和需要靶向抗氧化治疗的患者提供更个性化的方法。
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引用次数: 10
Serum uric acid level predicts the progression of amyotrophic lateral sclerosis following treatment with edaravone 血清尿酸水平预测依达拉奉治疗后肌萎缩侧索硬化的进展
IF 3.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-03-16 DOI: 10.1080/13510002.2022.2051964
Hee Jo Han, H. Shin, Young-Chul Choi, S. M. Kim, Seung Woo Kim
ABSTRACT Introduction Uric acid and edaravone might exert a neuroprotective effect in amyotrophic lateral sclerosis (ALS) by reducing oxidative stress. We analyzed whether the treatment effect of edaravone is pronounced in patients whose uric acid level increased after the treatment with edaravone. Materials and methods Forty patients with ALS who underwent treatment with edaravone were included. Baseline uric acid level and the rate of decline in uric acid after edaravone treatment were recorded. The rate of change of ALS functional rating scale-revised (ΔALSFRS-R/month) was calculated based on baseline ALSFRS-R score and ALSFRS-R score 6–24 weeks after the treatment. Results The serum uric acid levels decreased after treatment in 26 (65%) patients and increased in 12 (30%) patients. The ΔALSFRS-R/month was significantly faster in patients whose uric acid decreased (median 1.5 [Q1–Q3, 0.7–3.1]) than in patients whose uric acid increased (0.2 [0–1.0], p = 0.021). A high baseline uric acid level and low rate of decline in uric acid was associated with slower disease progression after adjusting for age, initial symptoms, and riluzole administration (p = 0.030 and p = 0.041, respectively). Discussion High baseline values and low rate of decline in uric acid may predict slow disease progression in ALS patients treated with edaravone.
尿酸和依达拉奉可能通过降低氧化应激对肌萎缩性侧索硬化症(ALS)发挥神经保护作用。我们分析依达拉奉治疗后尿酸水平升高的患者依达拉奉治疗效果是否显著。材料与方法选取40例接受依达拉奉治疗的ALS患者。记录基线尿酸水平和依达拉奉治疗后尿酸下降的速率。根据治疗后6-24周的基线ALSFRS-R评分和ALSFRS-R评分计算ALS功能评定量表修订后的变化率(ΔALSFRS-R/月)。结果治疗后血清尿酸水平下降26例(65%),升高12例(30%)。尿酸降低组患者ΔALSFRS-R/月(中位数为1.5 [Q1-Q3, 0.7-3.1])明显快于尿酸升高组患者(中位数为0.2 [0-1.0],p = 0.021)。在调整年龄、初始症状和利鲁唑给药后,高基线尿酸水平和低尿酸下降率与较慢的疾病进展相关(p = 0.030和p = 0.041)。高基线值和低尿酸下降率可能预测依达拉奉治疗的ALS患者疾病进展缓慢。
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引用次数: 5
The evolving role of long noncoding RNA HIF1A-AS2 in diabetic retinopathy: a cross-link axis between hypoxia, oxidative stress and angiogenesis via MAPK/VEGF-dependent pathway 长链非编码RNA HIF1A-AS2在糖尿病视网膜病变中的进化作用:通过MAPK/ vegf依赖途径在缺氧、氧化应激和血管生成之间形成交联轴
IF 3.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-03-14 DOI: 10.1080/13510002.2022.2050086
M. Atef, Noha M. Shafik, Y. Hafez, M. Watany, Amal A. Selim, Heba M. Shafik, Omnia Safwat El-Deeb
ABSTRACT Background Diabetic retinopathy (DR) signifies a frequent serious diabetic complication influencing retinal structure and function. Dysregulation of lncRNAs drives a wide array of human diseases especially diabetes; thus, we aimed to study lncRNA HIF1A-AS2 role and its interplay with hypoxia, oxidative stress (OS), and angiogenesis in DR. Materials and methods 60 DM patients in addition to 15 healthy subjects. were enrolled. LncRNA HIF1A-AS2 mRNA relative gene expression was assessed. Hypoxia inducible factor 1-alpha (HIF-1α), vascular endothelial growth factor (VEGF), mitogen activated protein kinase (MAPK), and endoglin levels were assessed. Detection of DNA damage using comet assay, and Redox status parameters were also detected. Results LncRNA HIF1A-AS2 expression was significantly increased in diabetic patients with the highest levels in proliferative DR patients. Moreover, HIFα, VEGF, MAPK, and Endogolin levels were significantly higher in the diabetic patients compared to control group with the highest levels in in proliferative DR patients. Significant DNA damage in comet assay was observed to be the highest in this group. Conclusion We observed for the first time the imminent role of long noncoding RNA HIF1A-AS2 in DR throughout its stages and its interplay with hypoxia, OS, and angiogenesis via MAPK/VEGF-dependent pathway.
背景:糖尿病视网膜病变(Diabetic retinopathy, DR)是一种常见的严重糖尿病并发症,影响视网膜结构和功能。lncrna的失调驱动了一系列人类疾病,尤其是糖尿病;因此,我们旨在研究lncRNA HIF1A-AS2在dr中的作用及其与缺氧、氧化应激(OS)和血管生成的相互作用。材料和方法60例糖尿病患者和15例健康受试者。被录取。检测LncRNA HIF1A-AS2 mRNA相对基因表达。评估缺氧诱导因子1- α (HIF-1α)、血管内皮生长因子(VEGF)、丝裂原活化蛋白激酶(MAPK)和内激素水平。采用彗星法检测DNA损伤,并检测氧化还原状态参数。结果LncRNA HIF1A-AS2在糖尿病患者中表达明显升高,在增生性DR患者中表达最高。此外,糖尿病患者的HIFα、VEGF、MAPK和Endogolin水平明显高于对照组,其中以增生性DR患者的水平最高。在彗星试验中观察到显著的DNA损伤在该组中最高。我们首次观察到长链非编码RNA HIF1A-AS2在DR的各个阶段的潜在作用,以及它通过MAPK/ vegf依赖通路与缺氧、OS和血管生成的相互作用。
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引用次数: 11
Modulating gut dysbiosis and mitochondrial dysfunction in oxazolone-induced ulcerative colitis: the restorative effects of β-glucan and/or celastrol 在恶唑酮诱导的溃疡性结肠炎中调节肠道生态失调和线粒体功能障碍:β-葡聚糖和/或celastrol的恢复作用
IF 3.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-03-04 DOI: 10.1080/13510002.2022.2046425
Omnia Safwat El-Deeb, Rasha Osama El-Esawy, H. Al-Shenawy, H. Ghanem
ABSTRACT Objectives Microbiome–Mitochondria interaction is gaining a significant attention; thus, studying its mechanism emerges as a must to provide restorative lines in managing diseases. The aim is to study the mechanistic effects of β-Glucan and/or Celastrol in oxazolone-induced ulcerative colitis (UC). Methods 75 Wistar rats were allocated into 5 equal groups. Group I: control group. Group II: UC group, Group III: β-Glucan-treated UC group, Group IV: Celastrol-treated UC group & Group V: mutual treatment group. All groups were subjected to the detection of free fatty acid receptor 2 (FFAR-2) and peroxisome proliferator-activated receptor gamma co-activator1α (PGC-1α) mRNA gene expressions. Citrate synthase (CS) activity, mitochondrial membrane potential (MMP), ATP concentration, reactive oxygen species (ROS) were detected. Trimethylamine N-oxide (TMAO) concentration was measured. Results After treatment we monitored significant upregulation of FFAR-2 and PGC-1α mRNA expression. Likewise, ATP level and CS activity were significantly increased. On the contrary, there was a significant lessening in ROS and TMAO levels with improvement of MMP. Conclusion Mutual use of β- Glucan and Celastrol had a greater effect than each alone against UC, which is considered a novel finding highlighting the ameliorative effects of this combined treatment in modulating Microbiome/Mitochondria axis, thus launching promising avenues for UC.
微生物组-线粒体相互作用正在获得显著的关注;因此,研究其机制成为提供疾病管理恢复性系的必要条件。目的是研究β-葡聚糖和/或Celastrol在恶唑酮诱导的溃疡性结肠炎(UC)中的作用机制。方法75只Wistar大鼠随机分为5组。第一组:对照组。II组:UC组,III组:β-葡聚糖处理UC组,IV组:celastrol处理UC组,V组:互治组。各组均检测游离脂肪酸受体2 (FFAR-2)和过氧化物酶体增殖物激活受体γ共激活物1α (PGC-1α) mRNA基因表达。检测枸橼酸合成酶(CS)活性、线粒体膜电位(MMP)、ATP浓度、活性氧(ROS)。测定三甲胺n -氧化物(TMAO)浓度。结果治疗后,我们观察到FFAR-2和PGC-1α mRNA表达明显上调。ATP水平和CS活性均显著升高。相反,随着MMP水平的提高,ROS和TMAO水平显著降低。结论β-葡聚糖和雷公藤红素联合使用对UC的治疗效果优于单独使用,这是一个新的发现,突出了这种联合治疗在调节微生物组/线粒体轴方面的改善作用,从而为UC的治疗开辟了新的途径。
{"title":"Modulating gut dysbiosis and mitochondrial dysfunction in oxazolone-induced ulcerative colitis: the restorative effects of β-glucan and/or celastrol","authors":"Omnia Safwat El-Deeb, Rasha Osama El-Esawy, H. Al-Shenawy, H. Ghanem","doi":"10.1080/13510002.2022.2046425","DOIUrl":"https://doi.org/10.1080/13510002.2022.2046425","url":null,"abstract":"ABSTRACT Objectives Microbiome–Mitochondria interaction is gaining a significant attention; thus, studying its mechanism emerges as a must to provide restorative lines in managing diseases. The aim is to study the mechanistic effects of β-Glucan and/or Celastrol in oxazolone-induced ulcerative colitis (UC). Methods 75 Wistar rats were allocated into 5 equal groups. Group I: control group. Group II: UC group, Group III: β-Glucan-treated UC group, Group IV: Celastrol-treated UC group & Group V: mutual treatment group. All groups were subjected to the detection of free fatty acid receptor 2 (FFAR-2) and peroxisome proliferator-activated receptor gamma co-activator1α (PGC-1α) mRNA gene expressions. Citrate synthase (CS) activity, mitochondrial membrane potential (MMP), ATP concentration, reactive oxygen species (ROS) were detected. Trimethylamine N-oxide (TMAO) concentration was measured. Results After treatment we monitored significant upregulation of FFAR-2 and PGC-1α mRNA expression. Likewise, ATP level and CS activity were significantly increased. On the contrary, there was a significant lessening in ROS and TMAO levels with improvement of MMP. Conclusion Mutual use of β- Glucan and Celastrol had a greater effect than each alone against UC, which is considered a novel finding highlighting the ameliorative effects of this combined treatment in modulating Microbiome/Mitochondria axis, thus launching promising avenues for UC.","PeriodicalId":21096,"journal":{"name":"Redox Report","volume":"89 2 1","pages":"60 - 69"},"PeriodicalIF":3.8,"publicationDate":"2022-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83629372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
The antioxidant effect of triptolide contributes to the therapy in a collagen-induced arthritis rat model. 三苯氧胺的抗氧化作用有助于治疗胶原蛋白诱发的大鼠关节炎模型。
IF 5.2 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.2004047
Guang-Min Yu, Li-Feng Zhou, Bi-Xia Zeng, Jing-Jun Huang, Xiao-Jun She

Background: As a chronic autoimmune disease, rheumatoid arthritis (RA) is related to oxidative stress, which may lead to the occurrence and persistence of inflammation in RA. The purpose of this study is to evaluate the potential antioxidant effect of triptolide in collagen-induced arthritis (CIA) rat model.

Methods: We examined the severity of arthritis, levels of local and systemic oxidative stress, periarticular bone erosion and weight of organs in CIA rats treated with triptolide.

Results: We found that triptolide decreased the paw thickness and clinical arthritis score, significantly. The mRNA expression and activity of myeloperoxidase and inducible nitric oxide synthase were remarkably decreased in the paws of the CIA rats after triptolide treatment. Triptolide significantly inhibited the levels of nitrite and nitrate in serum, as well as the urinary level of dityrosine. Triptolide treatment also markedly increased bone volume of tibia, but suppressed epiphyseal plate thickness of both femur and tibia. In addition, there was no significant difference in the weight of organs after the therapy, except decreased spleen weight.

Conclusions: These results suggested that the local and systemic oxidative stress was enhanced in the CIA rats and the therapeutic dose of triptolide had a definite antioxidant effect.

背景:类风湿性关节炎(RA)作为一种慢性自身免疫性疾病,与氧化应激有关,氧化应激可能导致RA炎症的发生和持续。本研究旨在评估三苯氧胺在胶原诱导的关节炎(CIA)大鼠模型中的潜在抗氧化作用:方法:我们检测了接受曲普内酯治疗的 CIA 大鼠的关节炎严重程度、局部和全身氧化应激水平、关节周围骨侵蚀和器官重量:结果:我们发现三苯氧胺能显著降低大鼠爪厚度和临床关节炎评分。三苯氧胺治疗后,CIA 大鼠爪部髓过氧化物酶和诱导型一氧化氮合酶的 mRNA 表达和活性明显降低。雷公藤内酯能明显抑制血清中亚硝酸盐和硝酸盐的含量以及尿液中地屈嗪的含量。曲托内酯还能明显增加胫骨的骨量,但抑制股骨和胫骨的骺板厚度。此外,治疗后除脾脏重量下降外,其他器官重量无明显差异:这些结果表明,CIA 大鼠的局部和全身氧化应激增强,而治疗剂量的曲普内酯具有明确的抗氧化作用。
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引用次数: 0
Neferine improves oxidative stress and apoptosis in benign prostate hyperplasia via Nrf2-ARE pathway. 莲子碱通过Nrf2-ARE途径改善良性前列腺增生的氧化应激和细胞凋亡。
IF 3.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1871814
Nabila Jahan, Apu Chowdhury, Ting Li, Ke Xu, Fen Wei, Sicen Wang

Background: Progression of Benign Prostate hyperplasia (BPH) is vulnerable to oxidative stress (OS) and prostatic enlargement among the aging males through apoptosis deregulation. Our present study aimed to investigate the effect of neferine (NF) in the regulation of oxidative stress and apoptosis in human BPH-1 cells.

Methods: BPH epithelial cell line BPH-1 was treated with NF for 24 and 48 h. To measure oxidative stress (OS) we investigated MDA, SOD, and GST expression along with Nrf2 and its downstream gene and protein expression. Cell proliferation and apoptosis regulation was assayed with respective methods.

Results: Investigation revealed NF remarkably activate Nrf2 and its downstream proteins HO-1 and NQO1 at 48 h more substantially. Nrf2/Keap1 relative gene and protein expression indicated that NF might trigger Nrf2 upregulation by decreasing Keap1 expression. Both NF concentrations (3 µM and 9 µM) were able to deplete ROS and lipid peroxidation, concurrently, up-regulated SOD and GST. NF reduced cell proliferation significantly along with the regulation of apoptotic proteins Bax, Bcl2, Cyt-C, Caspase 9, and Caspase 3 at the same time (48 h).

Conclusion: This study is the first to manifest that NF may potentially regulate BPH by counterbalancing between OS and apoptosis through the activation of Nrf2-ARE pathway.

背景:男性良性前列腺增生(BPH)在衰老过程中易受氧化应激(OS)和前列腺增大的影响。本研究旨在探讨neferine (NF)在调节人BPH-1细胞氧化应激和凋亡中的作用。方法:用NF处理BPH上皮细胞株BPH-1 24和48 h,测定氧化应激(OS)水平,检测MDA、SOD、GST以及Nrf2及其下游基因和蛋白的表达。用不同的方法检测细胞增殖和凋亡调节。结果:研究发现,NF在48 h显著激活Nrf2及其下游蛋白HO-1和NQO1。Nrf2/Keap1相关基因及蛋白表达表明,NF可能通过降低Keap1的表达而触发Nrf2上调。两种NF浓度(3µM和9µM)均能减少ROS和脂质过氧化,同时上调SOD和GST。NF可显著降低细胞增殖,同时调节凋亡蛋白Bax、Bcl2、Cyt-C、Caspase 9和Caspase 3 (48 h)。结论:本研究首次证实NF可能通过激活Nrf2-ARE通路,在OS和凋亡之间进行平衡,从而调控BPH。
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引用次数: 16
Blood pro-oxidant/antioxidant balance in young men with class II obesity after 20 sessions of whole body cryostimulation: a preliminary study. 20次全身冷冻刺激后II级肥胖青年男性血液促氧化剂/抗氧化剂平衡:一项初步研究
IF 3.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1881328
Wanda Pilch, Joanna Wyrostek, Anna Piotrowska, Olga Czerwińska-Ledwig, Roxana Zuziak, Ewa Sadowska-Krępa, Marcin Maciejczyk, Małgorzata Żychowska

Objectives: In obesity, there is a shift in the pro-oxidative-antioxidant balance towards the oxidationreactions. However, it has been shown that in people with normal body composition, after a series of whole-body cryotherapy (WBC), the balance shifts in the opposite direction. Design: The aim of the study was to assess the impact of 20 WBC treatments on blood pro-oxidative-antioxidant balance. Interventions: Study included 14 obese (BMI > 35) and 10 non-obese volunteers. Methods: The total antioxidative (TAS/TAC) and pro-oxidative status (TOS/TOC) in serum and activity of antioxidant enzymes in erythrocytes were determined before the first and 2 hours after the last cryostimulation. Results: In the obese group, a significantly higher level of TOS/TOC, and its significant decrease after the WBC series, was observed. Cryotherapy had no influence on TAS/TAC level which was similar in both groups. Changes in activity of antioxidant enzymes were multidirectional. An increase in CAT activity in the obese group was observed. OSI, both before and after a series of treatments, was significantly higher in obese subjects. Conclusions: A beneficial effect on the level of TOS/TOC and CAT activity was indicated, but the proposed number of treatments for patients with class II obesity turned out to be insufficient. Trial registration: Australian New Zealand Clinical Trials Registry identifier: ACTRN12619000524190.

目的:在肥胖中,促氧化-抗氧化平衡向氧化反应转变。然而,研究表明,在身体成分正常的人群中,经过一系列全身冷冻疗法(WBC)后,平衡向相反的方向转移。设计:本研究的目的是评估20种白细胞治疗对血液促氧化-抗氧化平衡的影响。干预措施:研究包括14名肥胖(BMI > 35)和10名非肥胖志愿者。方法:在末次冷冻刺激1 h和2 h后测定大鼠血清总抗氧化活性(TAS/TAC)、促氧化活性(TOS/TOC)和红细胞抗氧化酶活性。结果:肥胖组TOS/TOC水平明显升高,WBC系列检查后TOS/TOC水平明显降低。冷冻治疗对两组患者TAS/TAC水平无影响,差异无统计学意义。抗氧化酶活性的变化是多向的。观察到肥胖组的CAT活性增加。在一系列治疗之前和之后,肥胖受试者的OSI明显更高。结论:提示对TOS/TOC水平和CAT活性有有益的影响,但对II类肥胖患者提出的治疗数量不足。试验注册:澳大利亚新西兰临床试验注册标识:ACTRN12619000524190。
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引用次数: 10
Contribution of oxidative stress in the mechanisms of postoperative complications and multiple organ dysfunction syndrome. 氧化应激在术后并发症和多器官功能障碍综合征机制中的作用。
IF 3.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1891808
Javier Toro-Pérez, Ramón Rodrigo

Background: The extent of the damage following surgery has been subject of study for several years. Numerous surgical complications can impact postoperative quality of life of patients and even can cause mortality. Although these complications are generally due to multifactorial mechanisms, oxidative stress plays a key pathophysiological role. Moreover, oxidative stress could be an unavoidable effect derived even from the surgical procedure itself.

Methods: A systematic review was performed following an electronic search of Pubmed and ScienceDirect databases. Keywords such as sepsis, oxidative stress, organ dysfunction, antioxidants, outcomes in postoperative complications, among others, were used. Review articles were preferably used between the years 2015 onwards, not excluding older ones.

Results: The vast majority point to the role of oxidative stress in generating greater damage and worse prognosis in postoperative patients without the necessary care and precautions, taking importance on the use of antioxidants to prevent this problem.

Discussions: Oxidative stress represents a common final pathway related to pathological processes such as inflammation or ischemia-reperfusion, among others. The expression of greater severity of these complications can result in multiple organ dysfunction or sepsis. The aim of this study was to present an update of the role of oxidative stress on surgical postoperative complications.

背景:多年来,人们一直在研究手术后的损伤程度。许多手术并发症会影响患者术后的生活质量,甚至导致死亡。虽然这些并发症通常是由多因素机制造成的,但氧化应激在病理生理学中起着关键作用。此外,氧化应激甚至可能是手术过程本身产生的不可避免的影响:方法:通过对 Pubmed 和 ScienceDirect 数据库的电子检索,进行了系统性综述。关键词包括脓毒症、氧化应激、器官功能障碍、抗氧化剂、术后并发症的结果等。回顾性文章最好使用2015年以后的文章,不排除更早的文章:结果:绝大多数文章都指出氧化应激在术后患者未采取必要护理和预防措施的情况下造成更大损害和更差预后的作用,并重视使用抗氧化剂来预防这一问题:氧化应激是与炎症或缺血再灌注等病理过程相关的常见最终途径。这些并发症的严重程度可导致多器官功能障碍或败血症。本研究旨在介绍氧化应激对手术术后并发症的最新作用。
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引用次数: 0
Ascorbate-dependent and ascorbate-independent Mn porphyrin cytotoxicity: anticancer activity of Mn porphyrin-based SOD mimics through ascorbate-dependent and -independent routes. 抗坏血酸依赖性和非抗坏血酸依赖性锰卟啉细胞毒性:锰卟啉基SOD模拟物通过抗坏血酸依赖性和非依赖性途径的抗癌活性。
IF 3.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2021-12-01 DOI: 10.1080/13510002.2021.1917214
Bader Hasan, Artak Tovmasyan, Ines Batinic-Haberle, Ludmil Benov

Objective: The aim of this study was to investigate how modifications at the periphery of the porphyrin ring affect the anticancer activity of Mn porphyrins (MnPs)-based SOD mimics.

Methods: Six compounds: MnTE-2-PyP with a short ethyl chain on the pyridyl ring; MnTnHexOE-2-PyP and MnTnOct-2-PyP with linear 8-atom alkyl chains, but the former with an oxygen atom within the alkyl chain; MnTE-2-PyPhP and MnTPhE-2-PyP with pyridyl and phenyl substituents, were investigated. Cytotoxicity was studied using pII and MDA-MB-231 cancer cell lines. Viability was assessed by the MTT (3-[4,5-dimethylthiazol-2-yl)]-2,5-diphenyltetrazolium bromide) assay and cell proliferation was determined by the sulforhodamine B assay.

Results: Cellular uptake was increased with the increase of the lipophilicity of the compounds, whereas reduction potential (E½) of the Mn(III)/Mn(II) redox couple shifted away from the optimal value for efficient redox cycling with ascorbate, necessary for ROS production. Amphiphilic MnPs, however, exerted anticancer activity by a mechanism not involving ROS.

Conclusion: Two different processes account for MnPs cytotoxicity. MnPs with appropriate E½ act via a ROS-dependent mechanism. Amphiphilic MnPs with suitable structure damage sensitive cellular constituents, leading to the suppression of proliferation and loss of viability. Design of compounds interacting directly with sensitive cellular targets is highly promising in the development of anticancer drugs with high selectivity and specificity.

目的:本研究的目的是研究卟啉环外围修饰如何影响Mn卟啉(MnPs)基SOD模拟物的抗癌活性。方法:六种化合物:吡啶环上有短乙基链的MnTE-2-PyP;MnTnHexOE-2-PyP和mntnnoct -2- pyp具有线性的8原子烷基链,但前者在烷基链内有一个氧原子;研究了具有吡啶基和苯基取代基的MnTE-2-PyPhP和mntph -2- pyp。用pII和MDA-MB-231癌细胞系研究细胞毒性。采用MTT(3-[4,5-二甲基噻唑-2-基)]-2,5-二苯基溴化四唑)法测定细胞活力,采用硫代丹胺B法测定细胞增殖。结果:细胞摄取随着化合物亲脂性的增加而增加,而Mn(III)/Mn(II)氧化还原对的还原电位(E½)偏离了与抗坏血酸有效氧化还原循环的最佳值,这是ROS产生所必需的。然而,两亲性MnPs通过不涉及ROS的机制发挥抗癌活性。结论:两种不同的过程解释了MnPs的细胞毒性。具有适当E½的MnPs通过ros依赖机制起作用。具有合适结构的两亲性MnPs破坏敏感的细胞成分,导致增殖抑制和活力丧失。设计与敏感细胞靶点直接相互作用的化合物在开发具有高选择性和特异性的抗癌药物方面具有很大的前景。
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Redox Report
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