Pub Date : 2022-02-09DOI: 10.15825/1995-1191-2022-1-56-63
A. Shaikhaliev, M. S. Krasnov, E. V. Sidorsky, V. P. Yamskova, V. Lozinsky
Objective: to study the induction of osteogenesis caused by introducing into the defect area broadly porous cryogenically structured 3D carriers, based on serum albumin and loaded with a bioregulator isolated from bovine serum on an experimental model of mandible defect in rabbits in vivo.Materials and methods. Cryogenically structured sponges in the form of cylindrical specimens, 5 mm in diameter and 5 mm in height, prepared from bovine serum albumin, were used as the bioregulator carrier. The experimental laboratory animals were male Chinchilla rabbits, weighing 2–2.5 kg. Bone tissue was skeletonized under anesthesia (intramuscular anesthetic Zoletil 100) with a 3-cm incision in the angle of the mandible and a 5-mm-diameter cutter was used to create a 2–3-mm deep defect to install an appropriate-size albumin sponge. A total of 24 animals participated in the experiment. X-ray control of the defect area was performed in vivo on day 14 using PanExam+ (Kavo) device (20 m X-ray). Histological examination of tissues was carried out at day 30 after the defect using a light microscope.Results. Experiments performed indicate an active restoration of bone tissue in the extensive defect area when using an albumin-based 3D carrier with the inclusion of a bioregulator as compared to the control experiments. There were osteointegrative and osteoinductive processes, almost complete decomposition (biodegradation) of albumin sponge with formation of islands of dense bone tissue with small foci of coarse fibrous tissue in the defect. This demonstrated good dynamics of recovery processes at this stage of healing.Conclusion. Under the action of a serum bioregulator contained in an albumin-based sponge, the repair process leads to restoration of normal bone tissue without formation of bone callus and altered bone tissue different from the native one.
目的:研究在兔下颌骨缺损实验模型上，以血清白蛋白为基础，负载牛血清分离的生物调节剂，在缺损区引入宽孔低温结构三维载体对骨生成的诱导作用。材料和方法。以牛血清白蛋白为原料，制备直径5mm、高5mm的圆柱形低温结构海绵作为生物调节剂载体。实验动物为雄性栗鼠兔，体重2-2.5 kg。骨组织在麻醉下(肌内麻醉剂Zoletil 100)骨骼化，在下颌骨角度切开3cm切口，使用直径5mm的切割器形成2 - 3mm深的缺损，以安装合适大小的白蛋白海绵。共有24只动物参与了实验。第14天使用PanExam+ (Kavo)装置(20 m x线)对体内缺陷区域进行x线控制。缺损后第30天用光镜对组织进行组织学检查。所进行的实验表明，与对照实验相比，当使用包含生物调节剂的白蛋白基3D载体时，在广泛的缺陷区域中骨组织的主动修复。存在骨整合和骨诱导过程，白蛋白海绵几乎完全分解(生物降解)，缺损处形成密集骨组织岛状，粗纤维组织小灶。这表明在这个治疗阶段，恢复过程具有良好的动力学。在白蛋白海绵中含有的血清生物调节剂的作用下，修复过程导致正常骨组织的恢复，而不形成骨痂和改变与天然骨组织不同的骨组织。
{"title":"Induction of osteogenesis of the bone tissue Induction of osteogenesis in rabbit mandibular bone tissue using an albumin-based cryogenically structured porous 3D carrier loaded with a bioregulator","authors":"A. Shaikhaliev, M. S. Krasnov, E. V. Sidorsky, V. P. Yamskova, V. Lozinsky","doi":"10.15825/1995-1191-2022-1-56-63","DOIUrl":"https://doi.org/10.15825/1995-1191-2022-1-56-63","url":null,"abstract":"Objective: to study the induction of osteogenesis caused by introducing into the defect area broadly porous cryogenically structured 3D carriers, based on serum albumin and loaded with a bioregulator isolated from bovine serum on an experimental model of mandible defect in rabbits in vivo.Materials and methods. Cryogenically structured sponges in the form of cylindrical specimens, 5 mm in diameter and 5 mm in height, prepared from bovine serum albumin, were used as the bioregulator carrier. The experimental laboratory animals were male Chinchilla rabbits, weighing 2–2.5 kg. Bone tissue was skeletonized under anesthesia (intramuscular anesthetic Zoletil 100) with a 3-cm incision in the angle of the mandible and a 5-mm-diameter cutter was used to create a 2–3-mm deep defect to install an appropriate-size albumin sponge. A total of 24 animals participated in the experiment. X-ray control of the defect area was performed in vivo on day 14 using PanExam+ (Kavo) device (20 m X-ray). Histological examination of tissues was carried out at day 30 after the defect using a light microscope.Results. Experiments performed indicate an active restoration of bone tissue in the extensive defect area when using an albumin-based 3D carrier with the inclusion of a bioregulator as compared to the control experiments. There were osteointegrative and osteoinductive processes, almost complete decomposition (biodegradation) of albumin sponge with formation of islands of dense bone tissue with small foci of coarse fibrous tissue in the defect. This demonstrated good dynamics of recovery processes at this stage of healing.Conclusion. Under the action of a serum bioregulator contained in an albumin-based sponge, the repair process leads to restoration of normal bone tissue without formation of bone callus and altered bone tissue different from the native one.","PeriodicalId":21400,"journal":{"name":"Russian Journal of Transplantology and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79000144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-09DOI: 10.15825/1995-1191-2022-1-117-125
R. Muaid, T. Chesney
Background. Attitude towards organ donation is predominantly positive in the UK, however, donation rate remains low. To develop more effective interventions, this research aims to examine the behavioural barriers in organ donations using Q methodology to elicit patterns of overlap among different barriers and motivators.Method. A Q methodology study was conducted with 40 participants aged 19–64 were asked to rank 47 statements on issues that are associated with organ donation. By-person factor analysis using Centroid method and Varimax rotation was conducted to bring out patterns in the way statements were ranked to obtain groupings of participants who had arranged the statements in similar fashion.Results. Four viewpoints were extracted: The Realist, the Optimist Hesitant, the Pessimist Determinant and the Empathetic. Salient barriers to organ donation presented in each viewpoint suggest that perceived lack of knowledge, anxiety, mistrust in the healthcare system and lack of cue to action are the main barriers to organ donation. Consensus statements suggest that religion and family agreement are inconsequential if attitude to organ donation is well formed.Conclusion. There are different attitudes around deceased organ donation that were uncovered using Q methodology. These results suggest that people respond to behavioural change campaigns differently depending in their own perceptions on organ donation. We argue that a paradigm shift in behavioural interventions is underpinned by understanding the overlapping yet distinctive nature perceived perspectives.
{"title":"Q-Methodology to Identify perceptions of deceased organ donation in the UK","authors":"R. Muaid, T. Chesney","doi":"10.15825/1995-1191-2022-1-117-125","DOIUrl":"https://doi.org/10.15825/1995-1191-2022-1-117-125","url":null,"abstract":"Background. Attitude towards organ donation is predominantly positive in the UK, however, donation rate remains low. To develop more effective interventions, this research aims to examine the behavioural barriers in organ donations using Q methodology to elicit patterns of overlap among different barriers and motivators.Method. A Q methodology study was conducted with 40 participants aged 19–64 were asked to rank 47 statements on issues that are associated with organ donation. By-person factor analysis using Centroid method and Varimax rotation was conducted to bring out patterns in the way statements were ranked to obtain groupings of participants who had arranged the statements in similar fashion.Results. Four viewpoints were extracted: The Realist, the Optimist Hesitant, the Pessimist Determinant and the Empathetic. Salient barriers to organ donation presented in each viewpoint suggest that perceived lack of knowledge, anxiety, mistrust in the healthcare system and lack of cue to action are the main barriers to organ donation. Consensus statements suggest that religion and family agreement are inconsequential if attitude to organ donation is well formed.Conclusion. There are different attitudes around deceased organ donation that were uncovered using Q methodology. These results suggest that people respond to behavioural change campaigns differently depending in their own perceptions on organ donation. We argue that a paradigm shift in behavioural interventions is underpinned by understanding the overlapping yet distinctive nature perceived perspectives.","PeriodicalId":21400,"journal":{"name":"Russian Journal of Transplantology and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72883564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-09DOI: 10.15825/1995-1191-2022-1-96-106
A. Kostyunin
Current research shows that some of the pathogenetic processes behind structural destruction of bioprosthetic valves are largely similar to those involved in the development of atherosclerotic vascular lesions and native valve calcification. These processes include lipid and leukocyte infiltration, typical for both prosthetic and native tissues. They are accompanied by formation of foam cells, excessive production of matrix-degrading enzymes and increased oxidative stress. This fact suggests that some approaches to conservative treatment of atherosclerosis may be useful for prolonging the lifespan of bioprosthetic valves.
{"title":"Structural valve degeneration: are there common mechanisms with atherosclerosis and calcific aortic stenosis?","authors":"A. Kostyunin","doi":"10.15825/1995-1191-2022-1-96-106","DOIUrl":"https://doi.org/10.15825/1995-1191-2022-1-96-106","url":null,"abstract":"Current research shows that some of the pathogenetic processes behind structural destruction of bioprosthetic valves are largely similar to those involved in the development of atherosclerotic vascular lesions and native valve calcification. These processes include lipid and leukocyte infiltration, typical for both prosthetic and native tissues. They are accompanied by formation of foam cells, excessive production of matrix-degrading enzymes and increased oxidative stress. This fact suggests that some approaches to conservative treatment of atherosclerosis may be useful for prolonging the lifespan of bioprosthetic valves.","PeriodicalId":21400,"journal":{"name":"Russian Journal of Transplantology and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85998494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-03DOI: 10.15825/1995-1191-2022-1-89-95
E. G. Kuznetsova, O. M. Kuryleva, L. A. Salomatina, L. A. Kirsanova, Z. Z. Gonikova, A. Nikolskaya, N. Shmerko, V. Sevastianov
Introduction. The use of immunomodulators to regulate reparative processes in affected organs and tissues remains a pressing issue. Of greatest interest is liver regeneration after extended hepatic resection (EHR) in donors in right lobe living related donor liver transplantation. We propose a transdermal therapeutic system (TTS) with an immunomodulator to enhance the natural process of liver tissue regeneration. Objective: to study the effect of transdermal administration of immunomodulator sodium aminodihydrophthalazinedione on early recovery processes in the liver after EHR in in vivo experiments.Materials and methods. Sodium aminodihydrophthalazinedione was used as an active substance in TTS in the form of powder for preparation of intramuscular injection solution (Galavit®, SELVIM LLC). An experimental EHR model was performed on 22 male Wistar rats weighing 350–380 g. After HER, all animals were divided into two groups. Group 1 (n = 10) consisted of untreated animals. In group 2 (n = 12), TTS was applied immediately after liver resection. The experiment lasted for 48 hours; the TTS was changed once after 24 hours from the beginning of application.Results. In either group, there was no significant difference in the weight of liver remnant gain and in biochemical blood parameters at 48 hours after EHR. Assessment of the mitotic index (MI) of hepatocytes 48 hours after EHR revealed a significant increase in MI in both groups in comparison with the baseline (before liver resection) equal to 0.14 ± 0.07‰. The MI in group 1 and group 2 animals was 12.70 ± 4.9‰ and 17.43 ± 4.90‰, respectively (p ≤ 0.05).Conclusion. Studies on the regenerative activity of sodium aminodihydrophthalazinedione TTS on an experimental EHR model in rats showed that this drug form had a pronounced stimulating effect on the mitotic activity of liver cells.
{"title":"Effect of transdermal immunomodulation on liver regeneration","authors":"E. G. Kuznetsova, O. M. Kuryleva, L. A. Salomatina, L. A. Kirsanova, Z. Z. Gonikova, A. Nikolskaya, N. Shmerko, V. Sevastianov","doi":"10.15825/1995-1191-2022-1-89-95","DOIUrl":"https://doi.org/10.15825/1995-1191-2022-1-89-95","url":null,"abstract":"Introduction. The use of immunomodulators to regulate reparative processes in affected organs and tissues remains a pressing issue. Of greatest interest is liver regeneration after extended hepatic resection (EHR) in donors in right lobe living related donor liver transplantation. We propose a transdermal therapeutic system (TTS) with an immunomodulator to enhance the natural process of liver tissue regeneration. Objective: to study the effect of transdermal administration of immunomodulator sodium aminodihydrophthalazinedione on early recovery processes in the liver after EHR in in vivo experiments.Materials and methods. Sodium aminodihydrophthalazinedione was used as an active substance in TTS in the form of powder for preparation of intramuscular injection solution (Galavit®, SELVIM LLC). An experimental EHR model was performed on 22 male Wistar rats weighing 350–380 g. After HER, all animals were divided into two groups. Group 1 (n = 10) consisted of untreated animals. In group 2 (n = 12), TTS was applied immediately after liver resection. The experiment lasted for 48 hours; the TTS was changed once after 24 hours from the beginning of application.Results. In either group, there was no significant difference in the weight of liver remnant gain and in biochemical blood parameters at 48 hours after EHR. Assessment of the mitotic index (MI) of hepatocytes 48 hours after EHR revealed a significant increase in MI in both groups in comparison with the baseline (before liver resection) equal to 0.14 ± 0.07‰. The MI in group 1 and group 2 animals was 12.70 ± 4.9‰ and 17.43 ± 4.90‰, respectively (p ≤ 0.05).Conclusion. Studies on the regenerative activity of sodium aminodihydrophthalazinedione TTS on an experimental EHR model in rats showed that this drug form had a pronounced stimulating effect on the mitotic activity of liver cells.","PeriodicalId":21400,"journal":{"name":"Russian Journal of Transplantology and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91278703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-03DOI: 10.15825/1995-1191-2022-1-72-88
N. A. Onishchenko, Z. Z. Gonikova, A. Nikolskaya, L. A. Kirsanova, V. Sevastianov
Cell death represents the most critical pathologic entity in liver disease, which dictates pathologic consequences such as inflammation, fibrosis, and cell transformation. We analyzed the conclusions of studies on the involvement of different types of programmed cell death (PCD) in the pathogenesis of liver diseases. Three main forms of PCD (autophagy, apoptosis, necrosis) and five additional, still insufficiently studied PCD – necroptosis, ferroptosis, pyroptosis, partanatosis and entosis – observed in the liver in various acute and chronic diseases are considered. The involvement of several PCD at once in the development of any one pathology and one type of PCD in different pathologies was established. This indicates the existence of cross-regulation of metabolism in the liver cells with different levels of damage in the formation of the main dominant type of PCD. Available results indicate the possibility of attenuation (correction) of functional and morphological manifestations of PCD in the organ by controlled blocking of effector-mediated PCD pathways, as well as targeted induction of autophagy, anti-apoptotic and anti-necrotic mechanisms in liver cells.
{"title":"Programmed cell death and liver diseases","authors":"N. A. Onishchenko, Z. Z. Gonikova, A. Nikolskaya, L. A. Kirsanova, V. Sevastianov","doi":"10.15825/1995-1191-2022-1-72-88","DOIUrl":"https://doi.org/10.15825/1995-1191-2022-1-72-88","url":null,"abstract":"Cell death represents the most critical pathologic entity in liver disease, which dictates pathologic consequences such as inflammation, fibrosis, and cell transformation. We analyzed the conclusions of studies on the involvement of different types of programmed cell death (PCD) in the pathogenesis of liver diseases. Three main forms of PCD (autophagy, apoptosis, necrosis) and five additional, still insufficiently studied PCD – necroptosis, ferroptosis, pyroptosis, partanatosis and entosis – observed in the liver in various acute and chronic diseases are considered. The involvement of several PCD at once in the development of any one pathology and one type of PCD in different pathologies was established. This indicates the existence of cross-regulation of metabolism in the liver cells with different levels of damage in the formation of the main dominant type of PCD. Available results indicate the possibility of attenuation (correction) of functional and morphological manifestations of PCD in the organ by controlled blocking of effector-mediated PCD pathways, as well as targeted induction of autophagy, anti-apoptotic and anti-necrotic mechanisms in liver cells.","PeriodicalId":21400,"journal":{"name":"Russian Journal of Transplantology and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73370944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-02DOI: 10.15825/1995-1191-2022-1-126-136
R. Bahador, P. Mangolian, Jamileh Farokhzadian, S. S. Afrazandeh, Esmat Nouhi
Objectives: despite the annual increase in living donors and the positive and negative implications following organ donation, this issue had become a significant challenge for donors. The present study aimed to analyze the experiences and views of living donors to organ donation implications.Material and Methods. The present study was performed using qualitative content analysis. Twenty participants were selected using the purposive sampling method; data were collected by semi-structured interviews and analyzed based on Lundman and Graneheim contractual content analysis method after implementing MAX 12.Results. Data analysis elicitated 721 codes, 20 subcategories, six main categories, and two themes, including positive and negative implications of organ donation from the viewpoint of living donors. The main categories of positive effects resulting from organ donation included the «donor’s peace of mind», «fundamental strength», and «recipient’s achievements». On the other hand, the main categories of negative implications resulting from organ donation included «donor’s physical suffering», «damaged interactions», and «abandonment».Conclusion. Increasing the number of living donors makes us consider it essential to understand the efficiency of its two-way implications on many aspects of donor and recipient. Thus, managing the negative impacts of living organ donation and strengthening its positive side emphasizes the need to increase the awareness of organ donation associations, develop health policies at higher levels, and, most importantly, improve the satisfaction of live organ donors
{"title":"Analysis of implications of organ donation on living donors in southeastern Iran: A qualitative study","authors":"R. Bahador, P. Mangolian, Jamileh Farokhzadian, S. S. Afrazandeh, Esmat Nouhi","doi":"10.15825/1995-1191-2022-1-126-136","DOIUrl":"https://doi.org/10.15825/1995-1191-2022-1-126-136","url":null,"abstract":"Objectives: despite the annual increase in living donors and the positive and negative implications following organ donation, this issue had become a significant challenge for donors. The present study aimed to analyze the experiences and views of living donors to organ donation implications.Material and Methods. The present study was performed using qualitative content analysis. Twenty participants were selected using the purposive sampling method; data were collected by semi-structured interviews and analyzed based on Lundman and Graneheim contractual content analysis method after implementing MAX 12.Results. Data analysis elicitated 721 codes, 20 subcategories, six main categories, and two themes, including positive and negative implications of organ donation from the viewpoint of living donors. The main categories of positive effects resulting from organ donation included the «donor’s peace of mind», «fundamental strength», and «recipient’s achievements». On the other hand, the main categories of negative implications resulting from organ donation included «donor’s physical suffering», «damaged interactions», and «abandonment».Conclusion. Increasing the number of living donors makes us consider it essential to understand the efficiency of its two-way implications on many aspects of donor and recipient. Thus, managing the negative impacts of living organ donation and strengthening its positive side emphasizes the need to increase the awareness of organ donation associations, develop health policies at higher levels, and, most importantly, improve the satisfaction of live organ donors","PeriodicalId":21400,"journal":{"name":"Russian Journal of Transplantology and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81068979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-02DOI: 10.15825/1995-1191-2022-1-64-71
A. S. Ponomareva, N. Baranova, L. A. Kirsanova, G. N. Bubentsova, E. A. Nemets, I. Miloserdov, V. Sevastianov
Introduction. Developing a tissue-engineered pancreatic construct (TEPC) involves a search for matrices/scaffolds capable of mimicking the structure and composition of the natural extracellular matrix (ECM), which is an important component of the tissue microenvironment. A cell-free, tissue-specific matrix obtained from pancreas decellularization seems to be the most suitable for creation of a TEPC. The choice of pancreatic tissue decellularization protocol should take into account the morphological characteristics of the original pancreas. Preservation of the architectonics and composition of the native tissue in the decellularized pancreas matrix (DPM), and the presence of native ECM components allow for creation of conditions for prolonged vital activity of functionally active islet (insulin-producing) cells when creating TEPC.Objective: to determine the optimal parameters for decellularization of deceased donor pancreas with fibrosis, lipomatosis, and without pronounced signs of fibrosis and lipomatosis.Materials and methods. We used the caudal part of the pancreas obtained after multiorgan procurement from deceased donors, which was unsuitable for transplantation. Tissue-specific matrix was obtained by a combination of physical and chemical methods of pancreatic decellularization. A freeze-thaw cycle protocol and two protocols using osmotic shock were used. Samples of initial pancreatic tissue and decellularized fragments were subjected to histological analysis.Results. It was shown that a physico-chemical method with freeze-thaw cycles is suitable for effective pancreatic decellularization in severe lipomatosis; a physico-chemical method using osmotic shock, but different protocol variants, is suitable for pancreas with diffuse fibrosis and for pancreas without pronounced signs of fibrosis and lipomatosis.Conclusion. For complete human pancreatic decellularization, the protocol should be correlated with histological features of the original tissue.
{"title":"Determining the optimal pancreatic decellularization protocol, taking into account tissue morphological features","authors":"A. S. Ponomareva, N. Baranova, L. A. Kirsanova, G. N. Bubentsova, E. A. Nemets, I. Miloserdov, V. Sevastianov","doi":"10.15825/1995-1191-2022-1-64-71","DOIUrl":"https://doi.org/10.15825/1995-1191-2022-1-64-71","url":null,"abstract":" Introduction. Developing a tissue-engineered pancreatic construct (TEPC) involves a search for matrices/scaffolds capable of mimicking the structure and composition of the natural extracellular matrix (ECM), which is an important component of the tissue microenvironment. A cell-free, tissue-specific matrix obtained from pancreas decellularization seems to be the most suitable for creation of a TEPC. The choice of pancreatic tissue decellularization protocol should take into account the morphological characteristics of the original pancreas. Preservation of the architectonics and composition of the native tissue in the decellularized pancreas matrix (DPM), and the presence of native ECM components allow for creation of conditions for prolonged vital activity of functionally active islet (insulin-producing) cells when creating TEPC.Objective: to determine the optimal parameters for decellularization of deceased donor pancreas with fibrosis, lipomatosis, and without pronounced signs of fibrosis and lipomatosis.Materials and methods. We used the caudal part of the pancreas obtained after multiorgan procurement from deceased donors, which was unsuitable for transplantation. Tissue-specific matrix was obtained by a combination of physical and chemical methods of pancreatic decellularization. A freeze-thaw cycle protocol and two protocols using osmotic shock were used. Samples of initial pancreatic tissue and decellularized fragments were subjected to histological analysis.Results. It was shown that a physico-chemical method with freeze-thaw cycles is suitable for effective pancreatic decellularization in severe lipomatosis; a physico-chemical method using osmotic shock, but different protocol variants, is suitable for pancreas with diffuse fibrosis and for pancreas without pronounced signs of fibrosis and lipomatosis.Conclusion. For complete human pancreatic decellularization, the protocol should be correlated with histological features of the original tissue.","PeriodicalId":21400,"journal":{"name":"Russian Journal of Transplantology and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77989517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-02DOI: 10.15825/1995-1191-2022-1-143-150
A. Shabunin, M. Minina, P. V. Drozdov, I. Nesterenko, D. A. Makeev, O. S. Zhuravel, L. R. Karapetyan, S. А. Astapovich
Objective: to evaluate the safety and efficacy of hypothermic oxygenated machine perfusion (HOPE) for kidney grafts obtained from expanded criteria donors (ECD).Materials and methods. From June 2018 to June 2021, 200 surgeries involving kidney transplants from deceased donors were performed at Botkin City Clinical Hospital. Of these, 123 were men (61.5%) and 77 were women (38.5%). The mean age was 47.62 ± 11.69 (20–73) years. In 102 cases, kidney grafts were procured from ECD. In 92 recipients (90.2%) of kidney transplants from an expanded criteria donor, static cold storage done according to the standard technique was used to preserve the organ; these patients constituted observation group 1. In 10 recipients (9.8%), hypothermic oxygenated perfusion was used in addition to static cold preservation; these patients formed observation group 2.Results. No 30-day mortality was recorded in both observation groups. The mean static cold storage time in group 1 patients was 612.33 ± 178.88 (133–1180) minutes. Overall incidence of delayed graft function was 26.5% (53/200). Incidence of delayed graft function was 19.3% (19/98) for organs from standard donors using static cold storage and 35.8% (33/92) for ECD organs. Twenty-five patients (12.5%) had postoperative complications. Postoperative complications with delayed graft function were diagnosed in 12 patients, which was 22.6% (12/53), with immediate function in 13 patients, which was 8.8% (13/147). Mean cold storage time in group 2 patients was 319.11 ± 110.24 (311–525) minutes. Mean HOPE time was 202.34 ± 21.48 (150–210) minutes. Delayed graft function was recorded in 1 group 2 patient (10%). No complications, including perfusion-related one, were recorded in this group.Conclusion. The unique technique used at Botkin City Clinical Hospital for HOPE in kidney transplant is safe. It provides a low risk of delayed graft function for ECD kidneys.
{"title":"Early experiments with hypothermic oxygenated machine perfusion of kidney grafts from extended criteria donors","authors":"A. Shabunin, M. Minina, P. V. Drozdov, I. Nesterenko, D. A. Makeev, O. S. Zhuravel, L. R. Karapetyan, S. А. Astapovich","doi":"10.15825/1995-1191-2022-1-143-150","DOIUrl":"https://doi.org/10.15825/1995-1191-2022-1-143-150","url":null,"abstract":"Objective: to evaluate the safety and efficacy of hypothermic oxygenated machine perfusion (HOPE) for kidney grafts obtained from expanded criteria donors (ECD).Materials and methods. From June 2018 to June 2021, 200 surgeries involving kidney transplants from deceased donors were performed at Botkin City Clinical Hospital. Of these, 123 were men (61.5%) and 77 were women (38.5%). The mean age was 47.62 ± 11.69 (20–73) years. In 102 cases, kidney grafts were procured from ECD. In 92 recipients (90.2%) of kidney transplants from an expanded criteria donor, static cold storage done according to the standard technique was used to preserve the organ; these patients constituted observation group 1. In 10 recipients (9.8%), hypothermic oxygenated perfusion was used in addition to static cold preservation; these patients formed observation group 2.Results. No 30-day mortality was recorded in both observation groups. The mean static cold storage time in group 1 patients was 612.33 ± 178.88 (133–1180) minutes. Overall incidence of delayed graft function was 26.5% (53/200). Incidence of delayed graft function was 19.3% (19/98) for organs from standard donors using static cold storage and 35.8% (33/92) for ECD organs. Twenty-five patients (12.5%) had postoperative complications. Postoperative complications with delayed graft function were diagnosed in 12 patients, which was 22.6% (12/53), with immediate function in 13 patients, which was 8.8% (13/147). Mean cold storage time in group 2 patients was 319.11 ± 110.24 (311–525) minutes. Mean HOPE time was 202.34 ± 21.48 (150–210) minutes. Delayed graft function was recorded in 1 group 2 patient (10%). No complications, including perfusion-related one, were recorded in this group.Conclusion. The unique technique used at Botkin City Clinical Hospital for HOPE in kidney transplant is safe. It provides a low risk of delayed graft function for ECD kidneys.","PeriodicalId":21400,"journal":{"name":"Russian Journal of Transplantology and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74691409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-02DOI: 10.15825/1995-1191-2022-1-137-142
A. A. Anisimov, E. S. Gilmetdinova, M. A. Mulendeeva, A. Y. Anisimov
Objective: to study the views of the youth in the Republic of Tatarstan on organ donation and transplantation, to analyze their awareness and ideas about donor transplants and potential their willingness to become organ donors.Materials and methods. An anonymous sociological survey of 880 respondents aged 18 to 35 from the Republic of Tatarstan was conducted in the period from January 1 to July 1, 2021. An 11-question questionnaire was developed using online service Google Forms. Participation in the survey was voluntary.Results. Female and male respondents accounted for 79.0% and 21.0%, respectively; 34.2% of the respondents have or are receiving medical education. Among the respondents, 71.5% have a clear understanding of the term «organ donation», 27.4% are not sure of their understanding, 1.1% do not have a clear understanding. 56.8% consider the issue of organ donation for transplantation in the Republic of Tatarstan as a pressing matter, 3.5% do not see it so, while 39.7% found it difficult to answer. After death, 35.9% would agree to become donors, 39.5% probably would agree, 9.3% probably would disagree, 5.6% strongly disagrees, 9.7% found it difficult to answer. The most common associations with organ donation were positive: 34.5% associate it with «life», 25.1% with «help», and 22.0% with «lifeline».Conclusion. Young people in the Republic of Tatarstan are ready for a healthy debate of the problem of organ donation and most of them see it as a noble course. Given the interest in the problem and lack of awareness by the target audience, it is advisable to include independent academic disciplines on transplantology and organ donation in the curriculums of medical universities in the country. It is necessary to attract modern interdisciplinary information and educational resources to promote organ donation among the Russian public.
{"title":"Attitude of the youth in the Republic of Tatarstan towards organ donation","authors":"A. A. Anisimov, E. S. Gilmetdinova, M. A. Mulendeeva, A. Y. Anisimov","doi":"10.15825/1995-1191-2022-1-137-142","DOIUrl":"https://doi.org/10.15825/1995-1191-2022-1-137-142","url":null,"abstract":" Objective: to study the views of the youth in the Republic of Tatarstan on organ donation and transplantation, to analyze their awareness and ideas about donor transplants and potential their willingness to become organ donors.Materials and methods. An anonymous sociological survey of 880 respondents aged 18 to 35 from the Republic of Tatarstan was conducted in the period from January 1 to July 1, 2021. An 11-question questionnaire was developed using online service Google Forms. Participation in the survey was voluntary.Results. Female and male respondents accounted for 79.0% and 21.0%, respectively; 34.2% of the respondents have or are receiving medical education. Among the respondents, 71.5% have a clear understanding of the term «organ donation», 27.4% are not sure of their understanding, 1.1% do not have a clear understanding. 56.8% consider the issue of organ donation for transplantation in the Republic of Tatarstan as a pressing matter, 3.5% do not see it so, while 39.7% found it difficult to answer. After death, 35.9% would agree to become donors, 39.5% probably would agree, 9.3% probably would disagree, 5.6% strongly disagrees, 9.7% found it difficult to answer. The most common associations with organ donation were positive: 34.5% associate it with «life», 25.1% with «help», and 22.0% with «lifeline».Conclusion. Young people in the Republic of Tatarstan are ready for a healthy debate of the problem of organ donation and most of them see it as a noble course. Given the interest in the problem and lack of awareness by the target audience, it is advisable to include independent academic disciplines on transplantology and organ donation in the curriculums of medical universities in the country. It is necessary to attract modern interdisciplinary information and educational resources to promote organ donation among the Russian public.","PeriodicalId":21400,"journal":{"name":"Russian Journal of Transplantology and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72916254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-02DOI: 10.15825/1995-1191-2022-1-48-55
G. N. Skaletskaya, N. N. Skaletskiy, G. N. Bubentsova, V. Sevastianov
The success of pancreatic islet allotransplantation in the treatment of patients with a difficult-to-manage type 1 diabetes depends mainly on the quantity and quality of islets isolated from the pancreas of deceased donors using enzyme preparations, primarily collagenase. Numerous studies on improvement and standardization of islet isolation techniques have reached their limits in the last decade. This has made it impossible to further boost the number and quality of clinical transplants. Taking into account the negative impact of collagenase technique on the morphofunctional properties of isolated islets, this work has studied the possibility of enzyme-free isolation of islet tissue purified of exocrine ballast. Experiments using the pancreas of newborn and young rabbits showed that developing methodological approaches to obtaining islet-like cultures without the use of exogenous enzymes is feasible.
{"title":"Development of approaches to enzyme-free isolation of pancreatic islets","authors":"G. N. Skaletskaya, N. N. Skaletskiy, G. N. Bubentsova, V. Sevastianov","doi":"10.15825/1995-1191-2022-1-48-55","DOIUrl":"https://doi.org/10.15825/1995-1191-2022-1-48-55","url":null,"abstract":"The success of pancreatic islet allotransplantation in the treatment of patients with a difficult-to-manage type 1 diabetes depends mainly on the quantity and quality of islets isolated from the pancreas of deceased donors using enzyme preparations, primarily collagenase. Numerous studies on improvement and standardization of islet isolation techniques have reached their limits in the last decade. This has made it impossible to further boost the number and quality of clinical transplants. Taking into account the negative impact of collagenase technique on the morphofunctional properties of isolated islets, this work has studied the possibility of enzyme-free isolation of islet tissue purified of exocrine ballast. Experiments using the pancreas of newborn and young rabbits showed that developing methodological approaches to obtaining islet-like cultures without the use of exogenous enzymes is feasible.","PeriodicalId":21400,"journal":{"name":"Russian Journal of Transplantology and Artificial Organs","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90895088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：