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Evaluation of South African Chenin Blanc Wines Made From Six Different Trellising Systems Using a Chemical and Sensorial Approach 用化学和感官方法评价由六种不同网格系统制成的南非白葡萄酒
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-11-04 DOI: 10.21548/41-2-3889
Valeria Panzeri, H. N. Ipinge, A. Buica
There are many ways of manipulating the components of grape berries and one of these is the implementation of a specific trellising system. This will affect light exposure in the bunch zone, air flow through the canopy, crop load, etc., and consequently the primary metabolites that contribute to the production of secondary compounds in wine. The aim of the present study was to characterise the grape musts and wines of Chenin blanc made from grapes of different trellising systems, using chemical fingerprinting of the samples and the check-all-that-apply method, combined with a quality scoring test. The results indicate that, while the wines could not be separated according to treatment from an aroma point of view, the grapes produced by different trellis systems resulted in wines characterised by differences in taste and mouthfeel. The present study shows that trellising systems can influence amino acids, yeast assimilable nitrogen, phenolic content and aroma compounds, as well as sensory characteristics. In this case study, wine quality was not affected by the trellising systems, with one exception. Fingerprinting using high-resolution mass spectrometry proved to be a successful tool to separate the samples according to the systems.
有很多方法可以操作葡萄浆果的成分,其中之一就是实现特定的格架系统。这将影响束区的光照、穿过树冠的气流、作物负荷等,从而影响葡萄酒中产生次生化合物的主要代谢产物。本研究的目的是利用样品的化学指纹图谱和适用的检查方法,结合质量评分测试,对由不同格子系统的葡萄制成的Chenin blanc葡萄汁和葡萄酒进行表征。结果表明,虽然从香气的角度来看,葡萄酒不能根据处理方式进行分离,但不同格架系统生产的葡萄导致了葡萄酒在口感和口感上的差异。本研究表明,格架系统可以影响氨基酸、酵母可同化氮、酚类物质含量和香气化合物,以及感官特性。在这个案例研究中,葡萄酒质量没有受到格子系统的影响,只有一个例外。使用高分辨率质谱的指纹图谱被证明是根据系统分离样品的成功工具。
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引用次数: 3
Alteration Index Three Facilitates Interpretation of β-Glucosidase, Acid-phosphatase and Urease Activities in Soils Irrigated with Diluted Winery Wastewater 变化指数3有助于解释稀释酒厂废水灌溉土壤中β-葡萄糖苷酶、酸性磷酸酶和脲酶的活性
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-11-04 DOI: 10.21548/41-2-4195
I. van Huyssteen, A. R. Mulidzi, A. Meyer, J. Wooldridge
Soil enzyme activity data from a lysimeter pot trial involving four dissimilar soils irrigated over two simulated seasons (SS) with winery wastewater (WW) and municipal water (MW), were converted to numerical scores using the alteration index three (AI3). Unlike the activities of individual enzymes which are substrate specific and do not reflect the overall status of the soil microbiome, AI3 enabled differences in treatment-induced alteration states between combinations of soil and water to be quantified and statistically assessed. The more negative AI3 scores corresponded to a more altered state in the WW than the MW treatments, after SS3 than after SS4 and in the 0-10 cm compared to the 10-20 cm soil depth interval. AI3 is therefore a potentially useful adjunct to soil enzyme activity assays in monitoring and management of biological activity in vineyard soils. These findings, however, require verification under commercial vineyard conditions.
对四种不同土壤在两个模拟季节(SS)中分别用酒厂废水(WW)和市政用水(MW)灌溉的土壤进行了土壤酶活性盆栽试验,利用蚀变指数3 (AI3)将土壤酶活性数据转换为数值分数。不同于单个酶的活性是底物特异性的,不能反映土壤微生物组的整体状态,AI3可以量化和统计评估土壤和水组合之间处理诱导改变状态的差异。AI3得分越负,WW处理比MW处理、SS3处理比SS4处理、0-10 cm比10-20 cm土壤深度区间的变化状态越明显。因此,在葡萄园土壤生物活性的监测和管理中,AI3是土壤酶活性测定的潜在有用辅助手段。然而,这些发现需要在商业葡萄园条件下进行验证。
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引用次数: 6
Complete Genome Sequencing of Lactobacillus plantarum UNQLp 11 Isolated from a Patagonian Pinot Noir Wine 巴塔哥尼亚黑皮诺葡萄酒中植物乳杆菌UNQLp 11的全基因组测序
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-11-04 DOI: 10.21548/41-2-4066
N. G. Iglesias, N. Brizuela, E. Tymczyszyn, A. Hollmann, D. V. L. Hens, L. Semorile, B. Bravo-Ferrada
Lactobacillus plantarum UNQLp 11 strain was isolated from a Patagonian Pinot noir wine at the oldest commercial winery (110 years old) in General Roca, North Patagonia, Argentina, and has demonstrated its ability to survive during winemaking processes and successfully carry out malolactic fermentation. This work aimed to obtain the whole assembled genome of the UNQLp 11 strain, analysing its architecture and the possible functions of the predicted genes from the oenological properties of this strain. The genome size is 3 534 932 bp, with a mean GC content of 44.2%, 3 412 CDS, 80 transposons and 148 tandem repeats. A comparison between the genome size and gene content of 14 Lb. plantarum strains from different origins was performed, and UNQLp 11 exhibited the largest size. The in silico genome-wide analysis allowed us to confirm the existence of genes encoding enzymes involved in the synthesis of several metabolites of oenological interest, in addition to bacteriocins and exopolysaccharides. Furthermore, it is possible to speculate on this strain’s adaptation to different environments, as it is able to use diverse substrates for its growth. All these features suggest the potential of UNQLp 11 to be a good starter culture for malolactic fermentation.
植物乳杆菌UNQLp 11菌株是从阿根廷北巴塔哥尼亚General Roca最古老的商业酿酒厂(110年历史)的巴塔哥尼黑皮诺葡萄酒中分离出来的,并证明了其在酿酒过程中的生存能力,并成功地进行了苹果酸-乳酸发酵。这项工作旨在获得UNQLp 11菌株的完整基因组,从该菌株的酿酒学特性分析其结构和预测基因的可能功能。基因组大小为3 534 932 bp,平均GC含量为44.2%,共有3 412个CDS,80个转座子和148个串联重复序列。对来自不同来源的14株植物乳杆菌菌株的基因组大小和基因含量进行了比较,其中UNQLp 11的基因组大小最大。计算机全基因组分析使我们能够证实,除了细菌素和胞外多糖外,还存在编码酶的基因,这些酶参与了几种具有酿酒学意义的代谢产物的合成。此外,可以推测这种菌株对不同环境的适应,因为它能够使用不同的基质进行生长。所有这些特征表明,优衣库11有潜力成为苹果酸乳酸发酵的良好发酵剂。
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引用次数: 7
Evolution of Phenolic Composition During Barrel and Bottle Aging 桶和瓶老化过程中酚类成分的演变
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-11-04 DOI: 10.21548/41-2-4128
J. Aleixandré-Tudo, W. D. Toit
During red wine ageing, phenolic compounds undergo several reactions that have an impact on wine colour and mouthfeel properties. The evolution of phenolic content is affected during wine ageing. The aim of this study was to investigate the phenolic content and evolution of 82 commercial red wines subjected to barrel and bottle ageing. The phenolic content evolution of wines that underwent an ageing period of 12 months in commercial 225 litre barrels, followed by 12 months in the bottle, was monitored. While the total phenolic content remained stable, the anthocyanin fraction was affected the most, which led to substantial changes in the colour properties of the wines. Differences were found during both ageing regimes, indicating certain phenolic reactions being favoured or compromised under different ageing conditions, with an impact on wine colour properties. This paper provides the first large-scale study on the phenolic evolution of commercial red wine during ageing.
在红酒陈酿过程中,酚类化合物会发生一些反应,这些反应会影响葡萄酒的颜色和口感。葡萄酒陈化过程中酚类物质含量的变化受到影响。本研究的目的是调查82种商业红葡萄酒在桶和瓶陈化过程中的酚类物质含量和演变。对在商业225升桶中陈酿12个月,然后在瓶中陈酿12月的葡萄酒的酚含量变化进行了监测。虽然总酚含量保持稳定,但花青素部分受到的影响最大,这导致葡萄酒的颜色特性发生了实质性变化。在两种陈化过程中都发现了差异,这表明在不同的陈化条件下,某些酚类反应受到青睐或损害,对葡萄酒的颜色特性产生了影响。本文首次对商业红葡萄酒在陈化过程中的酚类物质演变进行了大规模研究。
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引用次数: 5
Modulation of Aroma and Sensory Properties of Prokupac Wines by a Bacillus-based Preparation Applied to Grapes Prior to Harvest 葡萄采收前芽胞杆菌制剂对Prokupac葡萄酒香气和感官特性的调节
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-11-04 DOI: 10.21548/41-2-4016
M. Malićanin, B. Danilović, D. Cvetković, Sandra Stamenkovic-Stojanovic, N. Nikolić, M. Lazić, Ivana T. Karabegović
Modern viticulture requires the replacement of hazardous agrochemicals with eco-friendly, bio-based products such as microbial preparations that  enhance grape and wine quality while protecting the grapevine from pest and disease attacks. This study investigated the effects of a commercially available Bacillusbased preparation on the volatile and sensory properties of wines made from Vitis vinifera, cv. Prokupac grapes. Three different concentrations of preparation based on Bacillus subtilis Ch-13 were applied to grapevines two weeks prior to harvest. The total soluble solids in the grapes was affected by the application of B. subtilis Ch-1 and the alcohol content of the wine made from these grapes was greater. Wines made from the B. subtilis Ch-13-treated grapes showed an average increase in total phenolic compounds of about 27%, compared to the wine made from the untreated control grapes. The colour intensity of wines from the treated grapes, independently of the concentration, was higher by more than 30% than for the wine from the control grape sample. The B. subtilis Ch-13 treatment also affected the content of 3-methyl-1-butanol, ethyl decanoate and ethyl octanoate in the wine, at about 35%, 40% and 20%, respectively. The latter compounds are responsible for floral and fruity aromas. Generally, wines made from the treated grapes showed similar sensorial characteristics but scored better overall than the control. Principal component analysis showed a clear differentiation between wine made from the control and that from the B. subtilis Ch-13-treated grapes. The results suggest that the application of B. subtilis Ch-13 to grapevines two weeks prior to harvest has a positive effect on wine quality.
现代葡萄栽培要求用生态友好的生物基产品取代有害的农用化学品,如微生物制剂,提高葡萄和葡萄酒的质量,同时保护葡萄藤免受病虫害的侵袭。本研究考察了市售芽孢杆菌制剂对葡萄(Vitis vinifera, cv.)酿造的葡萄酒挥发性和感官特性的影响。Prokupac葡萄。在葡萄收获前两周将三种不同浓度的枯草芽孢杆菌Ch-13制剂施用于葡萄。施施枯草芽孢杆菌Ch-1对葡萄的可溶性固形物总量有影响,使葡萄的酒精度提高。用枯草芽孢杆菌ch -13处理过的葡萄酿制的葡萄酒,其总酚类化合物的平均含量比用未处理过的对照葡萄酿制的葡萄酒高27%左右。经过处理的葡萄的葡萄酒的颜色强度,与浓度无关,比对照葡萄样品的葡萄酒高30%以上。枯草芽孢杆菌Ch-13对酒中3-甲基-1-丁醇、癸酸乙酯和辛酸乙酯的含量也有影响,分别为35%、40%和20%左右。后一种化合物负责花香和水果香味。一般来说,用处理过的葡萄酿制的葡萄酒表现出相似的感官特征,但总体得分高于对照。主成分分析表明,对照酒与经枯草芽孢杆菌ch -13处理的葡萄酒之间存在明显的差异。结果表明,采收前2周在葡萄上施用枯草芽孢杆菌Ch-13对葡萄酒品质有积极影响。
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引用次数: 5
Is There a Link Between Coffee Aroma and the Level of Furanmethanethiol (FMT) in Pinotage Wines 皮诺塔奇葡萄酒中呋喃甲硫醇(FMT)含量与咖啡香气有关系吗
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-11-04 DOI: 10.21548/41-2-4224
Gonzalo Garrido‐Bañuelos, A. Buica
Over the years, Pinotage has found its way into the South African and international market. Producers have used the flavour potential of this “original” South African grape to produce different wine styles, one of them being the so-called “coffee-style Pinotage”. The current study aims to explain the impact of furanmethanethiol (FMT) on the characteristic coffee aroma of these coffee-style wines. Chemical and sensory evaluation, as well as data mining of the technical information available, was performed. Not all wines marketed as “coffee Pinotage” showed a high “coffee” rating. However, the results showed a good correlation between the aroma perception and FMT concentrations (R2 = 0.81). However, RV coefficients were low when comparing the coffee rating with the information provided on both the front and the back label, which shows that, in some cases, the use of the “coffee Pinotage” term was rather part of the marketing strategy.
多年来,皮诺塔奇已经进入南非和国际市场。生产商利用这种“原产”南非葡萄的潜在味道,生产出不同风格的葡萄酒,其中一种就是所谓的“咖啡风格的皮诺塔奇”。目前的研究旨在解释呋喃甲硫醇(FMT)对这些咖啡型葡萄酒的特色咖啡香气的影响。进行了化学和感官评价,并对现有技术信息进行了数据挖掘。并非所有以“咖啡皮诺塔奇”(coffee Pinotage)销售的葡萄酒都显示出很高的“咖啡”评级。然而,结果表明,香气感知与FMT浓度之间存在良好的相关性(R2 = 0.81)。然而,当将咖啡等级与正面和背面标签上提供的信息进行比较时,RV系数很低,这表明,在某些情况下,使用“咖啡皮诺塔奇”一词是营销策略的一部分。
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引用次数: 1
Investigating the Concept of South African Old Vine Chenin Blanc 南非白诗南老葡萄的概念研究
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-11-04 DOI: 10.21548/41-2-4018
Mpho Mafata, J. Brand, Valeria Panzeri, A. Buica
Although South African vineyards are still young by European standards, there is a belief in the industry that vines aged 35 or more years produce grapes and wines with specific characteristics (“old vine wines”).  The aim of this study was to investigate the existence of the concept of old vine Chenin blanc wines using a typicality rating and sorting tasks. Chenin blanc wines were made from grapes harvested from vines aged five to 45 years old. Winemaking was standardised, with no wood contact. Typicality rating and sorting tasks were performed on young (first-stage) and two-year bottle-aged (second-stage) wines. Principal component analysis (PCA) on rating data demonstrated judge consensus, but no correlation was found between vine age and typicality rating. Sorting results were submitted to agglomerative hierarchical clustering (AHC) performed on the correspondence analysis (CA) and multidimensional scaling (MDS) results for grouping and attributes resulting from the sorting task. The clusters were different for the young wines and two-year bottle-aged wines. The verbal aspect of the sorting demonstrated the judges’ agreement on the concept of old vine Chenin blanc, shown by the annotation of the old vine group as ‘complex’, ‘balance’, ‘rich’ and ‘good mouthfeel’. However, because the judges did not sort the wines according to vine age, the perceptual aspect of the concept could not be confirmed, its features could not be tested further, and the sensory space could not be built.
尽管按照欧洲的标准,南非的葡萄园还很年轻,但业内有一种信念,即35年或以上的葡萄藤生产的葡萄和葡萄酒具有特定的特征(“老藤葡萄酒”)。本研究的目的是利用典型性评级和分类任务来调查陈年白诗南葡萄酒概念的存在。白诗南葡萄酒是用5 - 45年树龄的葡萄酿制而成。酿酒是标准化的,不使用木材。对年轻葡萄酒(第一阶段)和两年瓶装葡萄酒(第二阶段)进行了典型评级和分类任务。主成分分析(PCA)对评级数据进行了分析,得出了判断一致的结论,但葡萄树龄与典型性评级之间没有相关性。排序结果提交给对对应分析(CA)和多维缩放(MDS)结果进行分组和属性排序的聚合层次聚类(AHC)。年轻的葡萄酒和两年的瓶装葡萄酒的集群是不同的。评委会对陈年白诗南葡萄酒的评价是“复杂”、“平衡”、“丰富”和“口感好”,这体现了评委会对陈年白诗南葡萄酒概念的认同。然而,由于评委们并没有根据葡萄年份对葡萄酒进行分类,因此概念的感性方面无法得到确认,其特征也无法得到进一步的检验,感官空间也无法构建。
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引用次数: 4
Cold Hardiness of Primary Buds of Wine and Table Grape Cultivars in Poland 波兰葡萄酒和鲜食葡萄品种初芽抗寒性的研究
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-11-04 DOI: 10.21548/41-2-4041
J. Lisek, A. Lisek
The aim of this study was to compare the cold resistance of grapevine cultivars in field conditions. Following the winters of 2016/2017 and 2017/2018, an assessment of frost damage was carried out on the vines of 42 wine cultivars and 45 table grape cultivars grown in central Poland (Skierniewice, latitude 51.9627 N, longitude 20.1666 E). The minimum temperature for each of the two winters was recorded on 2017-01- 07 (-20.9°C) and on 2018-02-27 (-20.1°C). Among the assessed cultivars, 19 (13 wine grape cultivars and six table grape cultivars) belonged to V. vinifera species and 68 were inter- or inter-intraspecific hybrids.  Cultivars were divided into five classes of different frost tolerance, with information on the proportion of primary buds frozen given in the brackets: very resistant (below 1.9%), resistant (2% to 24.9%), medium susceptible (2% to 74.9%), susceptible (75% to 95.9%) and very susceptible (above 96%). The number of wine and table grape cultivars in particular classes (mean for two winters) was as follows: very resistant – 20 (wine) and 10 (table), resistant – 15 and 13, medium susceptible – six and 20, susceptible – one and two. Both the V. vinifera cultivars and the interspecific hybrids were highly diversified in terms of frost tolerance. In most cultivars, the number of frozen buds after the winter of 2016/2017 was greater than after the winter of 2017/2018. Among V. vinifera, ‘Riesling’, ‘Pinot Noir’, ‘Chasselas Dore’ (reference) and ‘Tauberschwarz’, ‘Veltliner Fruhrot’ (‘Fruehroter Malvasier’), ‘Turan’, ‘Domina’, ‘Tressot Panache’, ‘Blue from Tychy’, and ‘Irsai Oliver’ were very resistant or resistant. Other cultivars of V. vinifera were medium susceptible or susceptible.
本研究的目的是比较葡萄品种在田间条件下的抗寒性。继2016/2017年和2017/2018年冬季之后,对波兰中部(Skiernievice,北纬51.9627度,东经20.1666度)种植的42个葡萄酒品种和45个食用葡萄品种的葡萄藤进行了霜冻危害评估。两个冬季的最低温度分别记录在2017-01-07(-20.9°C)和2018-02-27(-20.1°C)。在评估的品种中,19个(13个酿酒葡萄品种和6个食用葡萄品种)属于葡萄品种,68个是种间或种内杂交品种。品种被分为五类不同的耐霜性,括号中给出了冷冻初生芽的比例信息:非常抗性(1.9%以下)、抗性(2%至24.9%)、中等敏感(2%至74.9%)、敏感(75%至95.9%)和非常敏感(96%以上)。葡萄酒和餐桌葡萄品种在特定类别中的数量(两个冬天的平均值)如下:非常抗性–20(葡萄酒)和10(餐桌),抗性–15和13,中等敏感–6和20,敏感–1和2。葡萄品种和种间杂交种在耐霜性方面都高度多样化。在大多数品种中,2016/2017年冬季之后的冷冻芽数量大于2017/2018年冬季之后。在葡萄中,“雷司令”、“黑皮诺”、“Chasselas Dore”(参考)和“Tauberschwarz”、“Veltliner Fruhrot Malvasier”、“Turan”、“Domina”、“Tressot Panache”、“Blue from Tychy”和“Irsai Oliver”具有很强的抗性或抗性。葡萄的其他品种是中等敏感或易感的。
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引用次数: 8
Colour Evaluation of Pinot Noir and Merlot Wines after Malolactic Fermentation Carried out by Oenococcus oeni and Lactobacillus plantarum Patagonian Native Strains 葡萄酒球菌和植物乳杆菌巴塔哥尼亚本地菌株苹果酸乳酸发酵后黑皮诺和梅洛葡萄酒的颜色评价
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-11-04 DOI: 10.21548/41-2-4069
N. Olguín, L. Delfederico, L. Semorile
Malolactic fermentation is a complex process that involves many reactions aside from the decarboxylation of L-malic acid. But we still have only glimpses of that complexity. It is not clear if the phenolic composition and colour are affected by malolactic fermentation and, if so, to what extent. So, the aims of this study were: 1) to evaluate the behaviour of native Patagonian strains of Oenococcus oeni and Lactobacillus plantarum in two wine varieties, and 2) to analyse the effect of malolactic fermentation on the colour of these wines.  Our results show that the survival of bacteria and L-malic acid decarboxylation is different depending on the lactic acid bacteria strain employed and the wine variety. In addition, we found that O. oeni can survive in wine even when L-malic acid is not being consumed. We found some correlations between MLF and colour-related parameters in Pinot noir but not for Merlot. In fact, some of the colour-related parameters measured in Merlot (total polyphenolic index, colour intensity, hue, as well as the CIELAB parameters) were affected even when L-malic acid was not being consumed.
苹果酸乳酸发酵是一个复杂的过程,除了l -苹果酸脱羧外,还涉及许多反应。但我们仍然只看到了这种复杂性的冰山一角。目前尚不清楚酚类成分和颜色是否受到苹果酸乳酸发酵的影响,如果有影响,影响程度如何。因此,本研究的目的是:1)评估本土巴塔哥尼亚葡萄球菌和植物乳杆菌菌株在两种葡萄酒品种中的行为,2)分析苹果酸乳酸发酵对这些葡萄酒颜色的影响。我们的研究结果表明,细菌的存活率和l -苹果酸脱羧作用取决于所使用的乳酸菌菌株和葡萄酒品种。此外,我们发现即使l -苹果酸没有被消耗,O. oeni也能在葡萄酒中存活。我们发现黑皮诺的MLF和颜色相关参数之间存在一些相关性,但梅洛没有。事实上,即使没有摄入l -苹果酸,梅洛中测量的一些与颜色相关的参数(总多酚指数、颜色强度、色调以及CIELAB参数)也会受到影响。
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引用次数: 3
Laboratory Bioassays on the Susceptibility of Trimen’s False Tiger Moth, Agoma trimenii (Lepidoptera: Agaristidae), to Entomopathogenic Nematodes and Fungi 三门假虎蛾(Agoma trimenii)对昆虫病原线虫和真菌敏感性的实验室生物测定
IF 1.3 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2020-11-04 DOI: 10.21548/41-2-4038
C. A. Morris, A. Malan, J. Waal, S. Johnson
Trimen’s false tiger moth, Agoma trimenii (Lepidoptera: Agaristidae), recently developed as a pest of grapevine in the Northern Cape and Limpopo (Groblersdal area) provinces of South Africa. Little is known about the biology of A. trimenii and control options are lacking. The aim of this study was to test the susceptibility of A. trimenii larvae and pupae to two locally isolated entomopathogenic nematodes (EPNs), Steinernema yirgalemense and Heterorhabditis noenieputensis , and two commercially available entomopathogenic fungi (EPF), Metarhizium anisopliae and Beauveria bassiana , under laboratory conditions. Larvae and pupae were screened for pathogenicity of the two nematode species, using a concentration of 100 infective juveniles (IJs)/50 μl of water. After 48 h, 100% mortality of the larval stage was found. However, no pupae were infected with EPNs. Larvae and pupae were screened for pathogenicity of the two EPF isolates by means of a dipping test, at a concentration of 0.2 ml/500 ml water and 0.5 g/500 ml water, respectively. Five days post-treatment, 100% larval mortality was recorded in comparison with no deaths in the controls. Overt mycosis was only observed in the case of M. anisopliae . However, in the case of pupae, no mortality was observed for both the nematode and the fungal applications. In future studies, the prepupal soil stage of A. trimenii should be screened for susceptibility to EPNs and EPF. The results of this study indicate the excellent potential of EPNs and EPF as biological control agents against the larvae of A. trimenii , especially for application to small areas with high infestation, without disrupting an integrated pest management programme.
Trimen的假虎蛾,Agoma trimenii(鳞翅目:银蛾科),最近在南非的北开普省和林波波省(Groblersdal地区)发展为葡萄的害虫。人们对三叶草的生物学知之甚少,也缺乏防治措施。在实验室条件下,研究了三叶草青虫幼虫和蛹对两种当地分离的昆虫病原线虫(steinnema yirgalemense和Heterorhabditis noenieputensis)以及两种市产昆虫病原真菌(Metarhizium anisopliae和Beauveria bassiana)的敏感性。对两种线虫的幼虫和蛹进行致病性筛选,浓度为100只感染幼虫/50 μl水。48 h后幼虫期死亡率100%。然而,没有蛹感染EPNs。采用浸渍法筛选两株EPF的幼虫和蛹的致病性,浸渍浓度分别为0.2 ml/500 ml水和0.5 g/500 ml水。治疗后5天,记录到100%的幼虫死亡率,而对照组没有死亡。仅在金龟子分枝杆菌病例中观察到明显的真菌病。然而,在蛹的情况下,没有观察到死亡的线虫和真菌应用。在今后的研究中,应筛选三叶草蛹前土壤阶段对epn和EPF的敏感性。本研究结果表明,EPNs和EPF作为三叶青蛾幼虫的生物防治剂具有良好的潜力,尤其适用于高虫害的小区域,而不会破坏害虫综合治理计划。
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引用次数: 2
期刊
South African Journal of Enology and Viticulture
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