Pub Date : 2023-09-26DOI: 10.24193/subbchem.2023.3.03
Doru Necula, Mădălina Ungureanu-Iuga, Sorin Daniel Dan, Octavia Tamas-Krumpe, Laurenț Ognean
{"title":"Analysis of the mineral profile of milk and Swiss cheese from Tara Dornelor in relation to seasonal and technological factors","authors":"Doru Necula, Mădălina Ungureanu-Iuga, Sorin Daniel Dan, Octavia Tamas-Krumpe, Laurenț Ognean","doi":"10.24193/subbchem.2023.3.03","DOIUrl":"https://doi.org/10.24193/subbchem.2023.3.03","url":null,"abstract":"","PeriodicalId":22005,"journal":{"name":"Studia Universitatis Babes-bolyai Chemia","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135720006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.24193/subbchem.2020.2.20
Mmoloki Makoba, Taboka Moalosi, P. Agachi, E. Muzenda, T. Mamvura
{"title":"Characterization of Botswana coal from two coal fields: Mabesekwa and Mmamabula to determine its coal rank","authors":"Mmoloki Makoba, Taboka Moalosi, P. Agachi, E. Muzenda, T. Mamvura","doi":"10.24193/subbchem.2020.2.20","DOIUrl":"https://doi.org/10.24193/subbchem.2020.2.20","url":null,"abstract":"","PeriodicalId":22005,"journal":{"name":"Studia Universitatis Babes-bolyai Chemia","volume":"1 1","pages":"243-256"},"PeriodicalIF":0.3,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89622218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-30DOI: 10.24193/subbchem.2020.1.10
G. Lazar, Călin Firta, Sanja Matić-Skoko, M. Peharda, Dario Vrdoljak, H. Uvanović, F. Nekvapil, B. Glamuzina, S. Pînzaru, Cluj-Napoca Romania Molecular Technologies, Šetalište I. Meštrovića Split Croatia Fisheries
Fish otoliths pose increasing interest due to their potential of rendering information about environmental changes, underlined in their non-linear time-dependent biogenic crystal growth. Otoliths are acellular, metabolically inert and continuously grow in a complex process which still needs to be understood. In the present work confocal Raman microspectroscopy (CRM) and imaging is employed to investigate the growth pattern in otoliths from Sparus aurata of Mediterranean provenance. CRM signal acquired from otolith sagittal section is exploited to associate it with the periodic growth increments denoted as rings. Raman signal collected from the core to the margins with micrometer spatial resolution invariably revealed characteristic signal of aragonite. Relative intensity variability was observed particularly for the lattice modes, indicating changes in crystalline orientation relative to incident laser. Bands associated with organic fraction were absent in the 90-1840 cm-1 spectral range. Daily growth rings were further studied using the Raman mapping of main aragonite bands intensities at 1083, 704 and the lattice modes in the 100-350 cm-1 range. The spectral intensity pattern closely follows the daily growth pattern. Traces of toxic or heavy metals incorporated in biogenic carbonate mineral were spuriously detected in the mapped areas, according to the position and width of the Raman bands of witherite (BaCO3), strontianite (SrCO3), along with the main aragonite and trace of its calcite polymorph.
{"title":"Tracking the growing rings in biogenic aragonite from fish otolith using confocal Raman microspectroscopy and imaging","authors":"G. Lazar, Călin Firta, Sanja Matić-Skoko, M. Peharda, Dario Vrdoljak, H. Uvanović, F. Nekvapil, B. Glamuzina, S. Pînzaru, Cluj-Napoca Romania Molecular Technologies, Šetalište I. Meštrovića Split Croatia Fisheries","doi":"10.24193/subbchem.2020.1.10","DOIUrl":"https://doi.org/10.24193/subbchem.2020.1.10","url":null,"abstract":"Fish otoliths pose increasing interest due to their potential of rendering information about environmental changes, underlined in their non-linear time-dependent biogenic crystal growth. Otoliths are acellular, metabolically inert and continuously grow in a complex process which still needs to be understood. In the present work confocal Raman microspectroscopy (CRM) and imaging is employed to investigate the growth pattern in otoliths from Sparus aurata of Mediterranean provenance. CRM signal acquired from otolith sagittal section is exploited to associate it with the periodic growth increments denoted as rings. Raman signal collected from the core to the margins with micrometer spatial resolution invariably revealed characteristic signal of aragonite. Relative intensity variability was observed particularly for the lattice modes, indicating changes in crystalline orientation relative to incident laser. Bands associated with organic fraction were absent in the 90-1840 cm-1 spectral range. Daily growth rings were further studied using the Raman mapping of main aragonite bands intensities at 1083, 704 and the lattice modes in the 100-350 cm-1 range. The spectral intensity pattern closely follows the daily growth pattern. Traces of toxic or heavy metals incorporated in biogenic carbonate mineral were spuriously detected in the mapped areas, according to the position and width of the Raman bands of witherite (BaCO3), strontianite (SrCO3), along with the main aragonite and trace of its calcite polymorph.","PeriodicalId":22005,"journal":{"name":"Studia Universitatis Babes-bolyai Chemia","volume":"1 1","pages":"125-136"},"PeriodicalIF":0.3,"publicationDate":"2020-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88731367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.24193/subbchem.2020.3.01
Laura Aquilante, M. Nistor, S. Taormina, M. Šebela, I. Frébort, I. Popescu
Grass pea amine oxidase (GPAO) and oat polyamine oxidase (OPAO) were immobilized along with horseradish peroxidase (HRP) and an Os-redox polymer (Os-RP) onto the surface of a graphite electrode by crosslinking with poly(ethylenglycol) diglycidyl ether. The resulted reagentless amperometric biosensors were inserted in a flow injection setup and used as electrochemical detectors for the biogenic amines (BA) detection. Both biosensors were operated at low applied potential (-50 mV vs. Ag/AgCl, KCl0.1M) where electrochemical interferences are minimal. The quantification of ten BA (tyramine, putrescine, cadaverine, histamine, cystamine, phenylethylamine, agmatine, tryptamine, spermine, and spermidine) either individual or in mixture (after a preliminary separation by using cation exchange chromatography) was reported. G/(Os-RP)-HRP-GPAO biosensor detected all ten BA, while G/(Os-RP)-HRP-OPAO biosensor detected only spermine and spermidine. Finally, a simple and low-cost method for free and acetylated polyamines determination in human urine samples, by using the highly selective G/(Os-RP)-HRP-OPAO biosensor, was proposed. (Less)
将草豆胺氧化酶(GPAO)和燕麦多胺氧化酶(OPAO)与辣根过氧化物酶(HRP)和os -氧化还原聚合物(Os-RP)通过聚乙二醇二甘油酯交联固定在石墨电极表面。所得的无试剂安培生物传感器被插入到流动注射装置中，用作生物胺(BA)检测的电化学检测器。两种生物传感器都在低施加电位(-50 mV vs. Ag/AgCl, KCl0.1M)下工作，电化学干扰最小。本文报道了10种BA(酪胺、腐胺、尸胺、组胺、半胺、苯乙胺、胍胺、色胺、精胺和亚精胺)的定量测定(用阳离子交换色谱法初步分离后)。G/(Os-RP)-HRP-GPAO生物传感器检测到所有10种BA，而G/(Os-RP)-HRP-OPAO生物传感器仅检测精胺和亚精胺。最后，利用高选择性的G/(Os-RP)-HRP-OPAO生物传感器，提出了一种简单、低成本的测定人尿样品中游离和乙酰化多胺的方法。(少)
{"title":"Determination of biogenic amines by using amperometric biosensors based on grass pea amine oxidase and oat polyamine oxidase","authors":"Laura Aquilante, M. Nistor, S. Taormina, M. Šebela, I. Frébort, I. Popescu","doi":"10.24193/subbchem.2020.3.01","DOIUrl":"https://doi.org/10.24193/subbchem.2020.3.01","url":null,"abstract":"Grass pea amine oxidase (GPAO) and oat polyamine oxidase (OPAO) were immobilized along with horseradish peroxidase (HRP) and an Os-redox polymer (Os-RP) onto the surface of a graphite electrode by crosslinking with poly(ethylenglycol) diglycidyl ether. The resulted reagentless amperometric biosensors were inserted in a flow injection setup and used as electrochemical detectors for the biogenic amines (BA) detection. Both biosensors were operated at low applied potential (-50 mV vs. Ag/AgCl, KCl0.1M) where electrochemical interferences are minimal. The quantification of ten BA (tyramine, putrescine, cadaverine, histamine, cystamine, phenylethylamine, agmatine, tryptamine, spermine, and spermidine) either individual or in mixture (after a preliminary separation by using cation exchange chromatography) was reported. G/(Os-RP)-HRP-GPAO biosensor detected all ten BA, while G/(Os-RP)-HRP-OPAO biosensor detected only spermine and spermidine. Finally, a simple and low-cost method for free and acetylated polyamines determination in human urine samples, by using the highly selective G/(Os-RP)-HRP-OPAO biosensor, was proposed. (Less)","PeriodicalId":22005,"journal":{"name":"Studia Universitatis Babes-bolyai Chemia","volume":"12 1","pages":"9-21"},"PeriodicalIF":0.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90426646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-30DOI: 10.24193/SUBBCHEM.2017.3.08
L. Rivoira, M. Žorž, M. Martelanc, S. Budal, Davide Carena, M. Franko, M. C. Bruzzoniti
In this work, new analytical approaches for determination of biogenic amines in wines were developed. For the first time, we studied the derivatization of BAs in wines with naphthalene- 2,3-dicarboxaldehyde (NDA) and with dabsyl chloride (DBS) and analysis of derivatized BAs by HPLC coupled to fluorescence (HPLC-NDA-FL) and thermal lens spectrometry (HPLC-DBS-TLS) detectors. The sensitivity of the two methods (LODs HPLC-NDA-FL in the range 27-73 μg/L; LODs HPLC-DBS-TLS in the range 3.4-11 μg/L) was higher than that of the official method for biogenic amines in wines, OIV-MA-AS315-18 (60-77 μg/L). For its best performances, the HPLC-DBS-TLS technique was applied to the analysis of putrescine, cadaverine, histamine and tyramine in two white wine samples. Additionally, exploiting the Berthelot reaction, the TLS fast screening of biogenic amines in wines, following the release of ammonia by transglutaminase, was also proposed. This approach allowed us to determine total biogenic amount content in concentrations below 0.1 mg/L, expressed as equivalents of histamine.
{"title":"Novel approaches for the determination of biogenic amines in food samples","authors":"L. Rivoira, M. Žorž, M. Martelanc, S. Budal, Davide Carena, M. Franko, M. C. Bruzzoniti","doi":"10.24193/SUBBCHEM.2017.3.08","DOIUrl":"https://doi.org/10.24193/SUBBCHEM.2017.3.08","url":null,"abstract":"In this work, new analytical approaches for determination of biogenic amines in wines were developed. \u0000For the first time, we studied the derivatization of BAs in wines with naphthalene- 2,3-dicarboxaldehyde (NDA) and with dabsyl chloride (DBS) and analysis of derivatized BAs by HPLC coupled to fluorescence (HPLC-NDA-FL) and thermal lens spectrometry (HPLC-DBS-TLS) detectors. The sensitivity of the two methods (LODs HPLC-NDA-FL in the range 27-73 μg/L; LODs HPLC-DBS-TLS in the range 3.4-11 μg/L) was higher than that of the official method for biogenic amines in wines, OIV-MA-AS315-18 (60-77 μg/L). For its best performances, the HPLC-DBS-TLS technique was applied to the analysis of putrescine, cadaverine, histamine and tyramine in two white wine samples. Additionally, exploiting the Berthelot reaction, the TLS fast screening of biogenic amines in wines, following the release of ammonia by transglutaminase, was also proposed. This approach allowed us to determine total biogenic amount content in concentrations below 0.1 mg/L, expressed as equivalents of histamine.","PeriodicalId":22005,"journal":{"name":"Studia Universitatis Babes-bolyai Chemia","volume":"13 1","pages":"103-122"},"PeriodicalIF":0.3,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83213344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.24193/SUBBCHEM.2017.4.38
Réka Kothencz, R. Nagy, L. Bartha
Advanced analytical and impact assessment methods are needed for the development of modern surfactants, so the composition, the effect and other properties of surfactants are examined by analytical tools which may be suitable for the quality testing of the raw materials, the intermediates and the final products. The HLB values of non-ionic surfactants and their mixtures were determined by three different methods: theoretical HLB based on the chemical structure and the given HLB of surfactants, the Greenwald method and an improved, titrimetric method. Our aim was to investigate if the new method to determine the water number and HLB value is suitable for this type of commercial surfactants and their mixtures. The particle size and the turbidity of micelles formed in surfactant solutions were also measured and evaluated.
{"title":"Determination of HLB values of some nonionic surfactants and their mixtures","authors":"Réka Kothencz, R. Nagy, L. Bartha","doi":"10.24193/SUBBCHEM.2017.4.38","DOIUrl":"https://doi.org/10.24193/SUBBCHEM.2017.4.38","url":null,"abstract":"Advanced analytical and impact assessment methods are needed for the development of modern surfactants, so the composition, the effect and other properties of surfactants are examined by analytical tools which may be suitable for the quality testing of the raw materials, the intermediates and the final products. The HLB values of non-ionic surfactants and their mixtures were determined by three different methods: theoretical HLB based on the chemical structure and the given HLB of surfactants, the Greenwald method and an improved, titrimetric method. Our aim was to investigate if the new method to determine the water number and HLB value is suitable for this type of commercial surfactants and their mixtures. The particle size and the turbidity of micelles formed in surfactant solutions were also measured and evaluated.","PeriodicalId":22005,"journal":{"name":"Studia Universitatis Babes-bolyai Chemia","volume":"2 1","pages":"451-458"},"PeriodicalIF":0.3,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73266592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.24193/SUBBCHEM.2017.3.16
C. Lungu, S. Ersali, Beata Szefler, Atena Pîrvan-Moldovan, S. Basak, M. Diudea
Dimensionality of a relatively big data set (95 compounds) observed for toxicity (mutagenicity) was explored in order to compute QSAR models. Distinct molecular descriptors were used. Dimensionality of data, using PCA, correlation plots and clustering, was evaluated. Analyzing data dimensionality allowed model optimization. Docking studies and PCA were used in order to expand data dimensionality. Pearson correlation coefficient (r) values, obtained for both perceptive and predictive models, were satisfactory.
{"title":"Dimensionality of big data sets explored by Cluj descriptors","authors":"C. Lungu, S. Ersali, Beata Szefler, Atena Pîrvan-Moldovan, S. Basak, M. Diudea","doi":"10.24193/SUBBCHEM.2017.3.16","DOIUrl":"https://doi.org/10.24193/SUBBCHEM.2017.3.16","url":null,"abstract":"Dimensionality of a relatively big data set (95 compounds) observed for toxicity (mutagenicity) was explored in order to compute QSAR models. Distinct molecular descriptors were used. Dimensionality of data, using PCA, correlation plots and clustering, was evaluated. Analyzing data dimensionality allowed model optimization. Docking studies and PCA were used in order to expand data dimensionality. Pearson correlation coefficient (r) values, obtained for both perceptive and predictive models, were satisfactory.","PeriodicalId":22005,"journal":{"name":"Studia Universitatis Babes-bolyai Chemia","volume":"32 1","pages":"197-204"},"PeriodicalIF":0.3,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77704209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.24193/SUBBCHEM.2017.2.16
Petra Herman, S. Harangi, M. Fehér, I. Fábián, E. Baranyai
{"title":"The development of analytical methods for the elemental determination of biotic indicators present in aquatic ecosystems","authors":"Petra Herman, S. Harangi, M. Fehér, I. Fábián, E. Baranyai","doi":"10.24193/SUBBCHEM.2017.2.16","DOIUrl":"https://doi.org/10.24193/SUBBCHEM.2017.2.16","url":null,"abstract":"","PeriodicalId":22005,"journal":{"name":"Studia Universitatis Babes-bolyai Chemia","volume":"129 4","pages":"213-222"},"PeriodicalIF":0.3,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72488590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-01-01DOI: 10.24193/SUBBCHEM.2017.3.25
A. Varga, Z. Bata, Pál Csuka, Diana Monica Bordea, B. Vértessy, A. Marcovici, F. Irimie, L. Poppe, L. Bencze
This study describes the cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from one liter of E. coli culture. Enzymatic activity measurements of the ammonia elimination reaction from different natural aromatic amino acids proved the protein to be a phenylalanine ammonia-lyase. The isolated protein showed remarkably high, 81.7 °C melting temperature, making it especially suitable for biocatalytic applications.
{"title":"A novel phenylalanine ammonia-lyase from Kangiella koreensis","authors":"A. Varga, Z. Bata, Pál Csuka, Diana Monica Bordea, B. Vértessy, A. Marcovici, F. Irimie, L. Poppe, L. Bencze","doi":"10.24193/SUBBCHEM.2017.3.25","DOIUrl":"https://doi.org/10.24193/SUBBCHEM.2017.3.25","url":null,"abstract":"This study describes the cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from one liter of E. coli culture. Enzymatic activity measurements of the ammonia elimination reaction from different natural aromatic amino acids proved the protein to be a phenylalanine ammonia-lyase. The isolated protein showed remarkably high, 81.7 °C melting temperature, making it especially suitable for biocatalytic applications.","PeriodicalId":22005,"journal":{"name":"Studia Universitatis Babes-bolyai Chemia","volume":"1 1","pages":"293-308"},"PeriodicalIF":0.3,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89875203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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