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KUALITAS BAKSO SAPI YANG DIOLAH DENGAN TEPUNG SORGUM MERAH (Sorghum bicolor L. Moench) SEBAGAI PENGGANTI TEPUNG TAPIOKA SELAMA MASA SIMPAN (Quality of beef meatballs which were proceded with red sorgum flour (Sorghum bicolor L. Moench) as a tapioca.....
Pub Date : 2022-06-30 DOI: 10.35508/nukleus.v9i1.5229
Gerfasius Ninu, G. Malelak, Heri Armadianto
This study aims to determine the quality of beef meatballs processed with red sorghum flour as a substitute for tapioca flour during the shelf life. This study used a completely randomized design with 5 treatments and 4 replications. The treatments consisted of TS0 = 100% tapioca, TS25 = 25% red sorghum flour, TS50 = 50% red sorghum flour, TS75 = 75% red sorghum flour; TS100 = 100% red sorghum flour. Furthermore, the variation of storage time is 1 day and 30 days. The variables observed were organoleptic (color, aroma, taste), TPC, fat oxidation and elasticity. The results of statistical analysis showed that the use of red sorghum flour as a substitute for tapioca flour up to 100% did not change the color of the meatballs at 1 day of storage, but at 30 days of storage the color of the meatballs darkened, the aroma of the meatballs became on either 1 day or 30 days of storage (P <0.01), the meatball taste score at 1 day of storage decreased and at 30 days the meatball taste score was between very dislike to dislike (P<0.05), the elasticity score decreased from very chewy to not chewy score (P< 0.01), while at 30 days of storage the elasticity score of the meatballs was the same, namely not chewy. The treatment had a very significant effect (P<0.01) on TPC and fat oxidation. This study concluded that the higher the percentage of replacement of red sorghum flour decreased the organoleptic qualities such as darkening of the color, the decrease in aroma, taste and elasticity of the meatballs, the TPC (Total Plate Count) number. and fat oxidation increased from 1 day to 30 days of storage.
以红高粱粉代替木薯粉加工牛肉肉丸,考察其保质期内的质量。本研究采用完全随机设计,5个处理,4个重复。TS0 = 100%木薯粉,TS25 = 25%红高粱粉,TS50 = 50%红高粱粉,TS75 = 75%红高粱粉;TS100 = 100%红高粱粉。贮藏时间的变化为1天和30天。观察到的变量包括感官(色、香、味)、TPC、脂肪氧化和弹性。统计分析的结果表明,使用红色高粱面粉代替木薯粉100%并没有改变的颜色肉丸在1天的存储,但在30天的存储肉丸昏暗的颜色、香气的肉丸成为1天或30天的存储(P < 0.01), 1天的肉丸味道得分存储下降和30天之间的肉丸味道得分是非常不喜欢不喜欢(P < 0.05),肉丸的弹性评分由极耐嚼降至不耐嚼(P< 0.01),贮藏30 d时肉丸的弹性评分不变,即不耐嚼。处理对TPC和脂肪氧化有极显著影响(P<0.01)。结果表明,红高粱粉替代率越高,肉丸的颜色变深,香气、口感和弹性降低,TPC (Total Plate Count)数减少。贮藏1 ~ 30 d脂肪氧化增加。
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引用次数: 2
KUALITAS SEMEN DOMBA LOKAL DARI FREKUENSI EJAKULASI BERBEDA (The quality of local ram semen from different ejaculation) 当地不同射精方式的公羊精液质量
Pub Date : 2022-06-30 DOI: 10.35508/nukleus.v9i1.6596
D. Setiadi, F. Fatimah, D. Diapari, R. Arifiantini
One of the factors that support the success of reproduction in sheep is the quality of the semen. This study aims to analyze the effect of ejaculation frequency on the quality of ram semen. This study used four local rams two years old. All rams have collected the semen using an artificial vagina five times a day with three replications. The experimental parameters were macroscopic (volume, pH, consistency, and color) and microscopic (mass movement, motility, viability, concentration, normal sperm morphology, and sperm intact plasma membrane). The data analysis used a randomized block design (RBD) with SPSS version 20. The results showed that the frequency of ejaculation in rams shows color and consistency in the 1st ejaculate, which was creamier and thicker than the 4th and 5th ejaculate. Mass movement, sperm concentration per mL, and total sperm concentration in ejaculate 1st and 2nd were higher than in 5th ejaculate. The potential of artificial insemination (AI) in the 1st and 2nd ejaculate was higher than in the 5th ejaculate. The study concluded that the frequency of ejaculation affects mass movement, sperm concentration, and the potential straw production for AI in local sheep.
绵羊繁殖成功的因素之一是精液的质量。本研究旨在分析射精频率对公羊精液质量的影响。这项研究使用了四只当地两岁的公羊。所有公羊每天用人工阴道收集5次精液,重复3次。实验参数为宏观(体积、pH、浓度、颜色)和微观(质量运动、活力、活力、浓度、正常精子形态、精子完整质膜)。数据分析采用随机区组设计(RBD),采用SPSS version 20软件。结果表明,公羊第一次射精时,射精的颜色和一致性较好,比第4次和第5次射精时更细腻、更厚。第1次和第2次射精的质量运动、每mL精子浓度和总精子浓度均高于第5次射精。第1次和第2次射精的人工授精潜力高于第5次射精。该研究得出结论,射精频率影响当地绵羊的质量运动、精子浓度和潜在的人工智能秸秆产量。
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引用次数: 0
PENGARUH PENAMBAHAN KUNYIT PUTIH (Curcuma zedoaria) TERHADAP AKTIVITAS AIR, TOTAL PLATE CONUT (TPC), STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI PADA TELUR ASIN (The influence of addition white tumeric (Curcuma zedoari) on water activity, total plate....
Pub Date : 2022-06-30 DOI: 10.35508/nukleus.v9i1.6736
Ermalinda Kasi, G. Malelak, Heri Armadianto, G. Oematan
Using of Curcuma zedoaria in food processing because of the presence of  bioactive compounds such as: phenols, flavonoids and tannins which act as antimicrobials.  The purposs of this experiment were to evaluate the effect of adding Curcuma zedoaria on salted egg quality.  Completely randomized design (CRD) 5 x 3 was used in this experiment.  The treatment used was the addition of white turmeric (Curcuma zedoaria) as much as 0%, 30%, 40%, 50% and 60%. Every treatment consisted of three replications.  Analysis of Variance (ANOVA) was used in this experiment followed by Duncan test to test the different among treatment.  The results showed that the addition of white turmeric to a level of 60% did not affect the water activity value (aw), causing an increasing in the TPC value, except for 50% (P<0.05), while Staphylococcus aureus and Escherichia coli were detected negative for all samples. The addition of white turmeric as much as 50%, gave the lowest TPC value. In processing salted eggs, it is necessary to ensure the age of the eggs used and storage methods so as to avoid contamination by microbes.
在食品加工中使用莪术,因为它含有生物活性化合物,如:酚类、类黄酮和单宁,它们具有抗菌剂的作用。本试验旨在评价添加莪术对咸蛋品质的影响。本试验采用完全随机设计(CRD) 5 × 3。白姜黄(Curcuma zedoaria)的添加量分别为0%、30%、40%、50%和60%。每个治疗包括三个重复。本试验采用方差分析(ANOVA),并采用Duncan检验检验各处理间的差异。结果表明:添加60%的白姜黄对水活度值(aw)无显著影响,除50%外,其余样品的TPC值均有升高(P<0.05),金黄色葡萄球菌和大肠杆菌均呈阴性。白姜黄添加量为50%时,TPC值最低。在咸蛋的加工过程中,要保证所用鸡蛋的年龄和储存方法,避免微生物的污染。
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引用次数: 0
KANDUNGAN ACID DETERGENT FIBER, NEUTRAL DETERGENT FIBER DAN SELULOSA HIJAUAN Indigofera zollingeriana PADA JENIS TANAH YANG BERBEDA (The content of acid detergent fiber, neutral detergent fiber and sellulose Indigofera zollingeriana forage in..... KANDUNGAN酸性洗涤纤维、中性洗涤纤维DAN SELULOSA HIJAUAN紫靛草paada JENIS TANAH YANG BERBEDA(紫靛草饲料中酸性洗涤纤维、中性洗涤纤维和纤维素洗涤纤维的含量在.....
Pub Date : 2022-06-30 DOI: 10.35508/nukleus.v9i1.6638
Amanda C. Lisu, H. P. Nastiti, B. B. Koten
This study was carried out the Forage Field Laboratory of faculty Animal Husbandry at the University of Nusa Cendana, Kupang for 5 months (September 2019 to January 2020). The purpose this study was to assess the effect Indigofera zollingeriana planted in different soil types on Acid Detergent Fiber (ADF), Neutral Detergent Fiber (NDF) and Cellulose. The experiment was followed a Completely Randomized Design (CRC) with 3 treatments and 6 replications. The treatments were: P1= Alluvial soil, P2= renzina soil and P3= Latosol soil. The variables measured were ADF, NDF and Cellulose. Planting Indigofera zollingeriana in different soil types increased the NDF (P<0,05) content but had not significant effect (P>0,05) on ADF and cellulose content. It can be concluded   that the NDF levels of Indigofera zollingeriana is determined by soil type Latosol produced the highest levels of NDF at 32,29%.
该研究在姑邦努沙仙丹那大学畜牧学院饲料野外实验室进行,为期5个月(2019年9月至2020年1月)。本试验旨在探讨不同土壤类型种植的紫靛对酸性洗涤纤维(ADF)、中性洗涤纤维(NDF)和纤维素的影响。试验采用完全随机设计(CRC), 3个处理,6个重复。处理分别为:P1=冲积土,P2=土积土,P3=红壤土。测定的变量为ADF、NDF和Cellulose。不同土壤类型种植蓝草对ADF和纤维素含量的NDF均有显著提高(p < 0.05)。综上所述,黄花靛蓝的NDF含量与土壤类型有关,红壤中NDF含量最高,为32.29%。
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引用次数: 0
KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS MODIFIKASI DENGAN WAKTU EKUILIBRASI YANG BERBEDA (The frozen sperm quality of duroc pig in modified tris diluent with different equilibration time) 杜洛克猪在不同同步时间内的冷冻水泥稀释剂(不同调谐时间的冷冻精子)
Pub Date : 2022-06-30 DOI: 10.35508/nukleus.v9i1.5491
Selsiana Dongkot, A. Marawali, T. Hine, W. M. Nalley
This study aimed to examine the quality of frozen sperm of duroc boar in modified Tris diluent with different equilibration time. Semen was collected from two duroc males and evaluated macroscopically and microscopically. Semen with motility 75%, concentration 200x106 cell/mL and abnormality <20% was diluted in Tris modified diluent, then held for two hours at room temperature (27-28ºC). The semen that has been in the holding time is then centrifuged at 2000 rpm for 15 minutes. The pellet from centrifuge were diluted again with modified Tris diluent + 3% glycerol. The diluted semen was packed in 0,50 mL mini straws and equilibrated according to the treatment equilibration time, namely P1: 1 hour equilibration time, P2: 2 hour equilibration time, P3: 3 hour equilibration time. After equilibration , it was frozen over liquid nitrogen vapor (-110ºC) at a distance of  5 cm for 10 minutes, then stored in liquid nitrogen (-196ºC) and left for 24 hours. Then thawing with warm water at 37ºC for 30 seconds in a water bath. The result showed that motility, viability, abnormality, intact plasma membrane and recovery rate were significantly  higher (P<0,05) at two hour equilibration , namely 23.12%, 45.44%, 4.65%, 50,15%, 33.03% respectively compared  to one and two hour equilibration time, namely 20%, 42.17%, 8.19%, 42.67%, 28.57% and 16,87%, 36.39%, 7.64%, 37.73%, 24.99% respectively. This study conclude that the equilibration time of two hours resulted in higher quality of frozen sperm of duroc boar than one and three hours.
本研究旨在考察杜洛克猪冷冻精子在不同平衡时间的改良Tris稀释液中的质量。采集了两只杜洛克雄性的精液,进行了宏观和微观评价。将活力75%、浓度200x106细胞/mL、异常<20%的精液稀释于Tris改性稀释液中,室温(27-28℃)保存2小时。保存时间内的精液以2000 rpm离心15分钟。用改良Tris稀释液+ 3%甘油再次稀释离心后的球团。将稀释后的精液装入0、50 mL迷你吸管中,按处理平衡时间P1: 1小时平衡时间,P2: 2小时平衡时间,P3: 3小时平衡时间进行平衡。平衡后,在液氮蒸气(-110ºC)上5 cm处冷冻10分钟,然后在液氮(-196ºC)中保存24小时。然后用37℃的温水在水浴中解冻30秒。结果表明,平衡2 h时的活力、活力、异常、完整质膜和恢复率分别比平衡1和2 h时的20%、42.17%、8.19%、42.67%、28.57%和16、87%、36.39%、7.64%、37.73%、24.99%显著提高(P< 0.05),分别为23.12%、45.44%、4.65%、50、15%、33.03%。结果表明,平衡时间为2小时的杜洛克公猪冷冻精子质量高于平衡时间为1小时和3小时的杜洛克公猪冷冻精子质量。
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引用次数: 0
PENGARUH SUBTITUSI SARI BUAH SEMANGKA (Citrullus lanatus) DALAM PENGENCER SITRAT- KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI (Effect of watermelon juice supplementation in citrate - egg yolk extender on spermatozoa quality of bali bulls)
Pub Date : 2022-06-30 DOI: 10.35508/nukleus.v9i1.4393
M. M. Bria, W. M. Nalley, J.N. Kihe, T. Hine
This study aims to determine the effect of adding watermelon juice (WJ) into citrate-egg yolk (C-EY) diluents on the spermatozoa quality of bali catlle. Semen was collected twice a week using an artificial vaginal method of three bali bulls with normal body condition and reproductive organs. Semen that has motility > 70%, concentration >1000 x 106 and abnormalities ≤ 20 % was used in this study. Semen then divided into five treatments: C-EY 100% (T0), C-EY-25% WJ (T1), C-EY-50% WJ (T2), C-EY-75% WJ (T3), WJ 100% (T4) and then stored in a refrigerator at temperature 3-5oC. The quality of spermatozoa was evaluated every 24 hours for sperm motility, viability, and abnormalities. The results showed that spermatozoa diluted with S-KT supplemented with 75% WJ (T3) had a higher quality (P<0,05) compared to the other treatments, with motility 40,53 %, viability 47,35%, and abnormalities 4,21% on the 6th day of preservation. It was concluded that the addition of WJ to C-EY diluent was able to maintain the quality of bali bull spermatozoa, with the best SBS concentration is 75%.
本试验旨在研究在柠檬酸-蛋黄稀释液中添加西瓜汁(WJ)对巴厘牛精子质量的影响。采用人工阴道法采集3头身体和生殖器官正常的巴厘公牛的精液,每周2次。精液活动力> 70%,浓度>1000 × 106,异常≤20%用于本研究。将精液分为5个处理:C-EY 100% (T0)、C-EY-25% WJ (T1)、C-EY-50% WJ (T2)、C-EY-75% WJ (T3)、WJ 100% (T4),保存在3-5℃的冰箱中。每24小时评估一次精子的活力、生存能力和异常情况。结果表明,S-KT加75% WJ (T3)稀释后的精子质量高于其他处理(P< 0.05),保存第6天的精子活力为40.53%,活力为47.35%,异常为4.21%。综上所述,在C-EY稀释液中添加WJ能够保持巴利牛精子的质量,SBS的最佳浓度为75%。
{"title":"PENGARUH SUBTITUSI SARI BUAH SEMANGKA (Citrullus lanatus) DALAM PENGENCER SITRAT- KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI (Effect of watermelon juice supplementation in citrate - egg yolk extender on spermatozoa quality of bali bulls)","authors":"M. M. Bria, W. M. Nalley, J.N. Kihe, T. Hine","doi":"10.35508/nukleus.v9i1.4393","DOIUrl":"https://doi.org/10.35508/nukleus.v9i1.4393","url":null,"abstract":"This study aims to determine the effect of adding watermelon juice (WJ) into citrate-egg yolk (C-EY) diluents on the spermatozoa quality of bali catlle. Semen was collected twice a week using an artificial vaginal method of three bali bulls with normal body condition and reproductive organs. Semen that has motility > 70%, concentration >1000 x 106 and abnormalities ≤ 20 % was used in this study. Semen then divided into five treatments: C-EY 100% (T0), C-EY-25% WJ (T1), C-EY-50% WJ (T2), C-EY-75% WJ (T3), WJ 100% (T4) and then stored in a refrigerator at temperature 3-5oC. The quality of spermatozoa was evaluated every 24 hours for sperm motility, viability, and abnormalities. The results showed that spermatozoa diluted with S-KT supplemented with 75% WJ (T3) had a higher quality (P<0,05) compared to the other treatments, with motility 40,53 %, viability 47,35%, and abnormalities 4,21% on the 6th day of preservation. It was concluded that the addition of WJ to C-EY diluent was able to maintain the quality of bali bull spermatozoa, with the best SBS concentration is 75%.","PeriodicalId":220267,"journal":{"name":"JURNAL NUKLEUS PETERNAKAN","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126635014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
ESTIMASI NILAI REPITABILITAS DAN PERFORMA PRODUKSI UMUR LAHIR DAN SAPIH INDUK BABI PERANAKAN LANDRACE (The estimate of repitability value and performances at birth and weaning age of landrace crossbred)
Pub Date : 2022-06-30 DOI: 10.35508/nukleus.v9i1.6245
Yohanes Djego, J. N. Kihe, Petrus Kune
The aims of this research  were to  estimate   repitability  coefficients of production  at birth  and  weaning characters  and to determine the average and standard deviation  of landrace  crossbreed sows. This research  was carried out in two breeding farms namely the Instalation of Pig Breeding, village of Tarus, Kupang Regency and the Manise Pig Farm, Village of Oetete, Kupang City, East  Nusa  Tenggara.   Materials used  15heads  landrace  crossbreed  sows ( 15  heads ) which born and weaned piglets  during the study periode.  The number of piglets for the first parity was 151heads and for the second parity was  162heads. All  sows  was  mated by artivicial insemination  with  boars of  landrace  crossbreed.   Variables were  liter size, weaning rate, birth weight, weaning  weight and weaning  rate.  Results  showed that landrace  crossbreed sows had the  average and  deviation standard for  the first and second liter size were 10.00±1.50heads  and  10.73±2.40heads, respectively;  for the first and second  birth weight were 1.37±0.23kg and 1.68±0.34kg, respectively;  for the first and second weaning  weight were  6.42±0.38kg  and  6.72±0.51kg, respectively  and for the first and second weaning rate were 8.33±1.28heads and  8.68±1.43 heads; respectively.  The average of litter size, birth weight, weaning weight and weaning rate between the first and second  parity  was no difference (P > 0,05). The  value of  repeatabitily for  characters  of  liter size, birth weight, weaning weight  and  weaning  rate   were  0.32; 0.47;  0.49 and 0,42; respectively.   In  conclusion,  the   repeatabily value   of litter  size,  birth weight, weaning  weight and weaning rate from landrace  crossbred sows were  medium value  and the performance of  those characters between  the first  and  second parity were  no difference.
本研究的目的是估计地方杂交母猪的出生和断奶特征的重复性系数,并确定平均和标准偏差。这项研究是在两个种猪场进行的,即库邦县Tarus村的猪场和东努沙登加拉库邦市Oetete村的Manise猪场。材料选用研究期间出生和断奶仔猪的地方杂交母猪15头(15头)。第一次胎次产仔151头,第二次胎次产仔162头。所有母猪均与地方杂交公猪进行人工授精配种。变量为升数、断奶率、出生体重、断奶重量和断奶率。结果表明:地方杂交种母猪一、二升头的平均和偏差标准分别为10.00±1.50头和10.73±2.40头;第一、二胎出生体重分别为1.37±0.23kg和1.68±0.34kg;第一次和第二次断奶体重分别为6.42±0.38kg和6.72±0.51kg,第一次和第二次断奶率分别为8.33±1.28头和8.68±1.43头;分别。第一胎和第二胎产仔数、初生重、断奶重和断奶率的平均值无显著差异(P > 0.05)。升体、初生重、断奶重、断奶率等性状的重复性值为0.32;0.47;0.49和0,42;分别。综上所述,地方杂交母猪产仔数、初生重、断奶重和断奶率的重复性值均为中等值,一胎和二胎的性能无显著差异。
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引用次数: 0
PENGARUH PERBEDAAN WAKTU EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER DURASPERM MODIFIKASI DENGAN AIR BUAH LONTAR (Effect of different equilibration time on the quality of landrace boar frozen semen on modification diluent....
Pub Date : 2022-06-30 DOI: 10.35508/nukleus.v9i1.4988
Maria G. Mega, W. M. Nalley, A. Marawali, Henederiana L. L. Belli
An experiment was carried out to recognize the effect of long equilibration time on the quality of spermatozoa and to obtain optimal equilibration time in the process cryopreservation of landrace pig semen. Semen was obtained from a landrace boar aged 2-3 years using glove hand method. The semen was than evaluated macroscopically and microscopically, good quality semen was diluted in a basic diluent, than kept for two hours at a temperature of 27-28oC. The semen were then centrifuged at 2000 rpm for 15 minutes, the supernatant was discarced and the pellets was re-diluted into basic diluent with a ratio of 1:1. Then, semen was diluted in treatment diluent with modified durasperm + 6% lactose + 4% glycerol with a concentration of 500 x 106/0.5 mL. Furthermore, the semen was packaged in 0.5 ml straws, then equilibrated at a temperature of 3-5oC appropriate to treatment, with 1 hour equilibration (T1), 2 hours equilibration (T2) and 3 hours equilibration (T3). After equilibration, the straws was frozen horizontally 5 cm above the liquid N2 vapor for 10 minutes at a temperature of ± -110oC and then stored in a liquid N2 container  (-196oC). To test frozen semen, it was done by stored frozen semen at 37oC for 30 seconds. The results showed that the highest quality of spermatozoa (P <0.05) was found in T2 and T3 when compared to T1 (P> 0.05), with the motility value was T2: 28.13 ± 2.39%; T3: 25.00 ± 4.08%; and T1 18,15±3,23%. It can be concluded that 2 hours and 3 hours equilibration time was able to maintain the quality of frozen semen landrace boars and the statistically was not significant.
通过实验研究了长时间的平衡时间对精子质量的影响,获得了长白猪精液冷冻保存过程中最佳的平衡时间。用手套手法从一只2-3岁的长白猪身上获得精液。将优质精液用碱性稀释液稀释后,在27 ~ 28℃的温度下保存2 h。将精液以2000 rpm离心15分钟,丢弃上清,将微球按1:1的比例重新稀释为碱性稀释液。然后,将精液用改性硬膜+ 6%乳糖+ 4%甘油稀释,浓度为500 × 106/0.5 mL。将精液包装在0.5 mL吸管中,在适合于处理的3-5℃温度下进行平衡,分别为1小时平衡(T1)、2小时平衡(T2)和3小时平衡(T3)。平衡后,在±-110℃的温度下,将秸秆在液氮蒸气上方5 cm处水平冷冻10分钟,然后保存在液氮容器(-196℃)中。为了测试冷冻精液,将冷冻精液在37℃下保存30秒。结果表明:精子质量最高(p0.05),运动值T2: 28.13±2.39%;T3: 25.00±4.08%;T1 18.15±3.23%。综上所述,2 h和3 h的平衡时间均能保持长白猪冷冻精液的质量,且差异无统计学意义。
{"title":"PENGARUH PERBEDAAN WAKTU EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER DURASPERM MODIFIKASI DENGAN AIR BUAH LONTAR (Effect of different equilibration time on the quality of landrace boar frozen semen on modification diluent....","authors":"Maria G. Mega, W. M. Nalley, A. Marawali, Henederiana L. L. Belli","doi":"10.35508/nukleus.v9i1.4988","DOIUrl":"https://doi.org/10.35508/nukleus.v9i1.4988","url":null,"abstract":"An experiment was carried out to recognize the effect of long equilibration time on the quality of spermatozoa and to obtain optimal equilibration time in the process cryopreservation of landrace pig semen. Semen was obtained from a landrace boar aged 2-3 years using glove hand method. The semen was than evaluated macroscopically and microscopically, good quality semen was diluted in a basic diluent, than kept for two hours at a temperature of 27-28oC. The semen were then centrifuged at 2000 rpm for 15 minutes, the supernatant was discarced and the pellets was re-diluted into basic diluent with a ratio of 1:1. Then, semen was diluted in treatment diluent with modified durasperm + 6% lactose + 4% glycerol with a concentration of 500 x 106/0.5 mL. Furthermore, the semen was packaged in 0.5 ml straws, then equilibrated at a temperature of 3-5oC appropriate to treatment, with 1 hour equilibration (T1), 2 hours equilibration (T2) and 3 hours equilibration (T3). After equilibration, the straws was frozen horizontally 5 cm above the liquid N2 vapor for 10 minutes at a temperature of ± -110oC and then stored in a liquid N2 container  (-196oC). To test frozen semen, it was done by stored frozen semen at 37oC for 30 seconds. The results showed that the highest quality of spermatozoa (P <0.05) was found in T2 and T3 when compared to T1 (P> 0.05), with the motility value was T2: 28.13 ± 2.39%; T3: 25.00 ± 4.08%; and T1 18,15±3,23%. It can be concluded that 2 hours and 3 hours equilibration time was able to maintain the quality of frozen semen landrace boars and the statistically was not significant.","PeriodicalId":220267,"journal":{"name":"JURNAL NUKLEUS PETERNAKAN","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123014175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
NILAI MIKROBIOLOGIS DAN OKSIDASI LEMAK SOSIS DARAH (TA’BU) YANG DIBERI TAMBAHAN PASTA ASAM (Tamarindus indica) (Microbiological and lipid oxidation value of blood sausage (ta'bu) which is additional with acid paste (tamarindus indica)
Pub Date : 2022-06-30 DOI: 10.35508/nukleus.v9i1.6727
Natalina E.E Gomes, G. Malelak, Heri Armadianto, G. Oematan
Ta'bu is a typical food in the Sabu Island area, which is made from blood and mixed with spices and then put into the large belly of the goat, then boiled. There are no studies that report on the quality of ta, ma'am.  The objective  of the study was to determine the microbiological quality and lipid oxidation value in traditional blood sausage (ta'bu) which was added tamarind paste. This study used a completely randomized design (CRD) 4 x 3 , where the 4 treatments consisted of P0 = without acid paste (kontrol); P1= 2% acid paste; P2= 4% acid paste; P3 = 6% acid paste.The parameters measured  were: TPC (Total Plate Count) value, Staphylococcus aureus , Salmonella sp  and lipid oxidation value. The data were analyzed using SPSS 21 ANOVA. The results showed that the addition of acid paste to ta’bu reduced the TPC numbers and lipid oxidation value (P<0.05), Staphylococus aureus was the same for all treatments and Salmonella sp was negative for all treatment. The results of this study concluded that adding tamarind paste in processing ta’bu can be used to reduce the value of TPC and lipid oxidation.
塔布是萨布岛地区的一种典型食物,它是用血和香料混合在一起,然后放入山羊的大肚子里,然后煮熟。没有研究报告说这是有质量的,女士。研究了罗望子酱对传统血肠(塔布)的微生物品质和脂质氧化值的影响。本研究采用完全随机设计(CRD) 4 × 3,其中4个处理包括:P0 =无酸膏(对照);P1= 2%酸膏;P2= 4%酸浆;P3 = 6%酸膏。检测参数为:TPC (Total Plate Count)值、金黄色葡萄球菌、沙门氏菌、脂质氧化值。数据采用SPSS 21方差分析。结果表明,酸膏的添加降低了TPC数量和脂质氧化值(P<0.05),金黄色葡萄球菌和沙门氏菌均呈阴性。本研究结果表明,在罗望子酱的加工中加入罗望子酱可以降低TPC值和脂质氧化。
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引用次数: 0
PENGARUH LAMA EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS-MODIFIKASI DENGAN PENAMBAHAN KRIOPROTEKTAN (Effect of equilibration time on frozen sement quality of the duroc pig in tris diluents modification With.....
Pub Date : 2022-06-30 DOI: 10.35508/nukleus.v9i1.5490
Biqshierz V.W Koelima, Henderiana L. L. Belli, J.N. Kihe, W. M. Nalley
The purpose of this study was to test the influence of the equilibration time on the frozen semen quality of duroc pig in modified tris-egg yolk with the addition of extracellular cryoprotectants. Semen is collected twice per week using massage methods from two duroc males aged ±2.5 years which in good health and have been trained in semen colection. The design used is a completely randomized design with 4 tests and 3 treatments T1: equilibration time of 1 hour, T2: equilibration time of 2 hours, T3: equilibration time of 3 hours at a temperature of 50C. Observed parameters were motility, viability, abnormality, MPU, and recovery rate (RR). The results showed that motility, viability, abnormalities, MPU, and RR were higher (P<0.05) at 2 hours of equilibration (25.63±3.15%, 55.86±5.41%, 4.92±0.53%, 56.69±5.64%, 36.61±4.49%) compared to 1 and 3 hours (20.00±3.54%, 39.28±9.89%, 7.93±0.76%, 40.21±9.39%, 28.57±5.05%) and (15.00±4.08%, 32.19±7.0%, 8.41±0.70%, 32.71±7.07%, 21.43±5.83%). It was concluded the motility of duroc pig spermatozoa in the tris-modified diluents after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the requirement of 40% spermatozoa motility for artificial insemination. It was concluded that the sperm motility of Duroc pig in tris-modified diluent after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the standard of 40% spermatozoa motility for artificial insemination.
本研究的目的是研究添加细胞外冷冻保护剂的杜洛克猪在改性三卵黄中,平衡时间对冷冻精液质量的影响。每周两次用按摩的方法收集两名年龄±2.5岁的杜洛克男性的精液,他们身体健康,接受过精液收集方面的培训。采用完全随机设计,4个试验,3个处理T1:平衡时间为1小时,T2:平衡时间为2小时,T3:平衡时间为3小时,温度为50℃。观察参数为运动、活力、异常、MPU和恢复率(RR)。结果显示,平衡后2小时的活动力、活力、异常、MPU、RR(25.63±3.15%、55.86±5.41%、4.92±0.53%、56.69±5.64%、36.61±4.49%)高于1、3小时(20.00±3.54%、39.28±9.89%、7.93±0.76%、40.21±9.39%、28.57±5.05%)和(15.00±4.08%、32.19±7.0%、8.41±0.70%、32.71±7.07%、21.43±5.83%),差异均有统计学意义(P<0.05)。综上所述,杜roc猪精子在三改性稀释液中平衡2 h后的活动力达到64.375%,解冻后达到25.63%,但未达到40%的人工授精要求。综上所述,杜洛克猪在三改性稀释液中平衡2 h后精子活力达到64.375%,解冻后达到25.63%,但未达到40%的人工授精精子活力标准。
{"title":"PENGARUH LAMA EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS-MODIFIKASI DENGAN PENAMBAHAN KRIOPROTEKTAN (Effect of equilibration time on frozen sement quality of the duroc pig in tris diluents modification With.....","authors":"Biqshierz V.W Koelima, Henderiana L. L. Belli, J.N. Kihe, W. M. Nalley","doi":"10.35508/nukleus.v9i1.5490","DOIUrl":"https://doi.org/10.35508/nukleus.v9i1.5490","url":null,"abstract":"The purpose of this study was to test the influence of the equilibration time on the frozen semen quality of duroc pig in modified tris-egg yolk with the addition of extracellular cryoprotectants. Semen is collected twice per week using massage methods from two duroc males aged ±2.5 years which in good health and have been trained in semen colection. The design used is a completely randomized design with 4 tests and 3 treatments T1: equilibration time of 1 hour, T2: equilibration time of 2 hours, T3: equilibration time of 3 hours at a temperature of 50C. Observed parameters were motility, viability, abnormality, MPU, and recovery rate (RR). The results showed that motility, viability, abnormalities, MPU, and RR were higher (P<0.05) at 2 hours of equilibration (25.63±3.15%, 55.86±5.41%, 4.92±0.53%, 56.69±5.64%, 36.61±4.49%) compared to 1 and 3 hours (20.00±3.54%, 39.28±9.89%, 7.93±0.76%, 40.21±9.39%, 28.57±5.05%) and (15.00±4.08%, 32.19±7.0%, 8.41±0.70%, 32.71±7.07%, 21.43±5.83%). It was concluded the motility of duroc pig spermatozoa in the tris-modified diluents after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the requirement of 40% spermatozoa motility for artificial insemination. It was concluded that the sperm motility of Duroc pig in tris-modified diluent after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the standard of 40% spermatozoa motility for artificial insemination.","PeriodicalId":220267,"journal":{"name":"JURNAL NUKLEUS PETERNAKAN","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115534976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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