Pub Date : 2015-05-14DOI: 10.1097/00007611-196604000-00035
J. D. Steele
{"title":"Lung Cancer. Surgery and Survival","authors":"J. D. Steele","doi":"10.1097/00007611-196604000-00035","DOIUrl":"https://doi.org/10.1097/00007611-196604000-00035","url":null,"abstract":"","PeriodicalId":22303,"journal":{"name":"The American review of respiratory disease","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83634926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-14DOI: 10.1164/ARRD.1965.91.3.445
P. Luchsinger
{"title":"Lungen- und Brustfellerkrankungen in Praxis und Klinik","authors":"P. Luchsinger","doi":"10.1164/ARRD.1965.91.3.445","DOIUrl":"https://doi.org/10.1164/ARRD.1965.91.3.445","url":null,"abstract":"","PeriodicalId":22303,"journal":{"name":"The American review of respiratory disease","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89482710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-14DOI: 10.1164/ARRD.1986.134.6.1196
J. Patel, E. Block
Nitrogen dioxide (NO2), an environmental oxidant pollutant, is toxic to lung cells. One of the postulated mechanisms of NO2-induced pulmonary injury involves peroxidation of membrane lipids. Therefore, we evaluated the effect of 5 ppm NO2 exposure on membrane lipid fluidity, uptake of 5-hydroxytryptamine (5-HT), lactate dehydrogenase (LDH) release, and formation of lipid peroxides in porcine pulmonary artery and aortic endothelial cells in culture. After 3- to 24-h exposure, cells were labeled with 1,6-diphenyl-1,3,5-hexatriene (DPH), an aromatic hydrocarbon that partitions into the hydrophobic interior of the lipid bilayer of cell membranes. Membrane fluidity was monitored by measuring changes in rotational relaxation time (rho) for DPH by fluorescence spectroscopy. Reductions in membrane fluidity increase the value of rho. The 5-HT uptake was calculated from the disappearance of 1 X 10(-6) M 14C-5-HT from the medium, and LDH release and lipid peroxide formation were measured by spectrophotometric methods. The NO2 caused a significant increase in rhoDPH in both types of endothelial cells after 3 h and progressed with further exposure to NO2. Exposure to NO2 for 24 h, but not 3 or 12 h, significantly (p less than 0.05) reduced 5-HT uptake, increased (p less than 0.01) LDH release, and increased (p less than 0.05) lipid peroxide formation in both pulmonary artery and aortic endothelial cells. These results suggest that oxidant injury caused by NO changes the physical state of membrane lipids, impairs membrane function, and contributes to the biochemical and metabolic abnormalities in the cells.
二氧化氮(NO2)是一种环境氧化污染物，对肺细胞有毒。二氧化氮诱导肺损伤的假设机制之一与膜脂过氧化有关。因此，我们评估了5ppm NO2暴露对培养的猪肺动脉和主动脉内皮细胞膜脂流动性、5-羟色胺(5- ht)摄取、乳酸脱氢酶(LDH)释放和脂质过氧化物形成的影响。暴露3至24小时后，细胞用1,6-二苯基-1,3,5-己三烯(DPH)标记，DPH是一种芳香烃，可进入细胞膜脂质双分子层的疏水内部。通过荧光光谱法测量DPH旋转弛豫时间(rho)的变化来监测膜的流动性。膜流动性的降低增加了rho的值。通过1 X 10(-6) M 14C-5-HT从培养基中消失计算5-HT的摄取，并通过分光光度法测量LDH释放和脂质过氧化形成。NO2在3小时后引起两种类型内皮细胞rhoDPH的显著增加，并随着进一步暴露于NO2而进展。暴露于NO2 24 h，而不是3或12 h，显著(p < 0.05)降低了肺动脉和主动脉内皮细胞的5-HT摄取，增加了LDH释放(p < 0.01)，增加了脂质过氧化形成(p < 0.05)。上述结果提示，一氧化氮引起的氧化损伤改变了细胞膜脂质的物理状态，损害了细胞膜功能，导致细胞生化代谢异常。
{"title":"Nitrogen dioxide-induced changes in cell membrane fluidity and function.","authors":"J. Patel, E. Block","doi":"10.1164/ARRD.1986.134.6.1196","DOIUrl":"https://doi.org/10.1164/ARRD.1986.134.6.1196","url":null,"abstract":"Nitrogen dioxide (NO2), an environmental oxidant pollutant, is toxic to lung cells. One of the postulated mechanisms of NO2-induced pulmonary injury involves peroxidation of membrane lipids. Therefore, we evaluated the effect of 5 ppm NO2 exposure on membrane lipid fluidity, uptake of 5-hydroxytryptamine (5-HT), lactate dehydrogenase (LDH) release, and formation of lipid peroxides in porcine pulmonary artery and aortic endothelial cells in culture. After 3- to 24-h exposure, cells were labeled with 1,6-diphenyl-1,3,5-hexatriene (DPH), an aromatic hydrocarbon that partitions into the hydrophobic interior of the lipid bilayer of cell membranes. Membrane fluidity was monitored by measuring changes in rotational relaxation time (rho) for DPH by fluorescence spectroscopy. Reductions in membrane fluidity increase the value of rho. The 5-HT uptake was calculated from the disappearance of 1 X 10(-6) M 14C-5-HT from the medium, and LDH release and lipid peroxide formation were measured by spectrophotometric methods. The NO2 caused a significant increase in rhoDPH in both types of endothelial cells after 3 h and progressed with further exposure to NO2. Exposure to NO2 for 24 h, but not 3 or 12 h, significantly (p less than 0.05) reduced 5-HT uptake, increased (p less than 0.01) LDH release, and increased (p less than 0.05) lipid peroxide formation in both pulmonary artery and aortic endothelial cells. These results suggest that oxidant injury caused by NO changes the physical state of membrane lipids, impairs membrane function, and contributes to the biochemical and metabolic abnormalities in the cells.","PeriodicalId":22303,"journal":{"name":"The American review of respiratory disease","volume":"69 1","pages":"1196-202"},"PeriodicalIF":0.0,"publicationDate":"2015-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89869665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-14DOI: 10.1007/springerreference_224848
J. B. Murray
{"title":"Mechanisms of Acute Respiratory Failure","authors":"J. B. Murray","doi":"10.1007/springerreference_224848","DOIUrl":"https://doi.org/10.1007/springerreference_224848","url":null,"abstract":"","PeriodicalId":22303,"journal":{"name":"The American review of respiratory disease","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76748612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-14DOI: 10.1097/00000441-196407000-00052
G. Hobby
{"title":"Germfree Life and Gnotobiology","authors":"G. Hobby","doi":"10.1097/00000441-196407000-00052","DOIUrl":"https://doi.org/10.1097/00000441-196407000-00052","url":null,"abstract":"","PeriodicalId":22303,"journal":{"name":"The American review of respiratory disease","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79120138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-14DOI: 10.1164/arrd.1974.110.3.367
S. Godfrey
{"title":"Ventilatory Response to Hypoxia","authors":"S. Godfrey","doi":"10.1164/arrd.1974.110.3.367","DOIUrl":"https://doi.org/10.1164/arrd.1974.110.3.367","url":null,"abstract":"","PeriodicalId":22303,"journal":{"name":"The American review of respiratory disease","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75230858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-14DOI: 10.7326/0003-4819-52-4-943_2
D. S. Lukas
{"title":"Symposium on Pulmonary Ventilation","authors":"D. S. Lukas","doi":"10.7326/0003-4819-52-4-943_2","DOIUrl":"https://doi.org/10.7326/0003-4819-52-4-943_2","url":null,"abstract":"","PeriodicalId":22303,"journal":{"name":"The American review of respiratory disease","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73683919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-14DOI: 10.7326/0003-4819-56-5-816_1
D. Roseman
{"title":"Current Therapy—1962","authors":"D. Roseman","doi":"10.7326/0003-4819-56-5-816_1","DOIUrl":"https://doi.org/10.7326/0003-4819-56-5-816_1","url":null,"abstract":"","PeriodicalId":22303,"journal":{"name":"The American review of respiratory disease","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79936997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-14DOI: 10.1007/978-3-642-53058-6
E. Kraft
{"title":"Differentialdiagnose Seltener Lungenerkrankungen im Röntgenbild","authors":"E. Kraft","doi":"10.1007/978-3-642-53058-6","DOIUrl":"https://doi.org/10.1007/978-3-642-53058-6","url":null,"abstract":"","PeriodicalId":22303,"journal":{"name":"The American review of respiratory disease","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72887364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-14DOI: 10.1164/arrd.1960.81.1P1.112
D. Salkin, J. D. Steele
{"title":"Surgery in tuberculosis.","authors":"D. Salkin, J. D. Steele","doi":"10.1164/arrd.1960.81.1P1.112","DOIUrl":"https://doi.org/10.1164/arrd.1960.81.1P1.112","url":null,"abstract":"","PeriodicalId":22303,"journal":{"name":"The American review of respiratory disease","volume":"1 1","pages":"112-3"},"PeriodicalIF":0.0,"publicationDate":"2015-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90770629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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