Pub Date : 2008-12-11DOI: 10.1111/J.1399-0039.1977.TB01123.X
E. B. Jensen, T. Kristensen, F. Jørgensen, L. Lamm
Several normalization procedures have been applied to single MLC-typing experiments in order to neutralize experimental variation and hence to secure assignment of HLA-D phenotypes. The present paper concerns a statistical description and isolation of experimental and biological factors in repeated MLC-typing experiments. The statistical model used gives a meaningful description of the variables in MLC as judged by its application to MLC-typing data of HLA-identical siblings. Subsequent testing of the model in an HLA-A, -B,-C and -D-genotyped family material provides evidence that the level of typing response to selected homozygous typing cells may be influenced by factors coded for by the homologous HLA-D region. It is, however, concluded that such evidence must be accepted with caution due to the (for the present statistical purpose) suboptimal design for the HLA-D typing experiments and hence the relative insensitivity of the method. A general method for locating erroneous triplicates is devised.
{"title":"HLA-D typing by homozygous typing cells. A statistical analysis of experimental and biological variation.","authors":"E. B. Jensen, T. Kristensen, F. Jørgensen, L. Lamm","doi":"10.1111/J.1399-0039.1977.TB01123.X","DOIUrl":"https://doi.org/10.1111/J.1399-0039.1977.TB01123.X","url":null,"abstract":"Several normalization procedures have been applied to single MLC-typing experiments in order to neutralize experimental variation and hence to secure assignment of HLA-D phenotypes. The present paper concerns a statistical description and isolation of experimental and biological factors in repeated MLC-typing experiments. The statistical model used gives a meaningful description of the variables in MLC as judged by its application to MLC-typing data of HLA-identical siblings. Subsequent testing of the model in an HLA-A, -B,-C and -D-genotyped family material provides evidence that the level of typing response to selected homozygous typing cells may be influenced by factors coded for by the homologous HLA-D region. It is, however, concluded that such evidence must be accepted with caution due to the (for the present statistical purpose) suboptimal design for the HLA-D typing experiments and hence the relative insensitivity of the method. A general method for locating erroneous triplicates is devised.","PeriodicalId":23105,"journal":{"name":"Tissue antigens","volume":"10 2 1","pages":"83-98"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1399-0039.1977.TB01123.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62840667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-12-11DOI: 10.1111/J.1399-0039.1976.TB00576.X
J. Marcusson, A. Elman, E. Möller, N. Thyresson
The present family investigation has shown that genes within the MHS are mainly responsible for the development of psoriasis or psoriasis-associated arthritic lesions (peripheral arthritis and sacroiliitis). We have hypothetically discussed the possibility that multiple genes, all located within the MHS, act in concert to increase the risk of developing disease to very high levels. This implies that at least two MHS linked genes act in complementary fashion for the development of disease, these genes seem to be able to operate both in the cis and in the trans position. One of these genes would be situated in the chromosomal portion of the MHS which carries the HLA-D locus. Families with a high incidence of disease would show inheritance according to the cis position of genes, when it can be shown that most of the carriers of the specific disease-associated haplotype are affected by disease, whereas in other families, complementarity between two distinct HLA haplotypes with genes acting in the trans position would result in disease.
{"title":"Psoriasis, sacro-iliitis and peripheral arthritis occurring in patients with the same HLA haplotype. A preliminary family report and a hypothetical explanation of the interaction between MHS products.","authors":"J. Marcusson, A. Elman, E. Möller, N. Thyresson","doi":"10.1111/J.1399-0039.1976.TB00576.X","DOIUrl":"https://doi.org/10.1111/J.1399-0039.1976.TB00576.X","url":null,"abstract":"The present family investigation has shown that genes within the MHS are mainly responsible for the development of psoriasis or psoriasis-associated arthritic lesions (peripheral arthritis and sacroiliitis). We have hypothetically discussed the possibility that multiple genes, all located within the MHS, act in concert to increase the risk of developing disease to very high levels. This implies that at least two MHS linked genes act in complementary fashion for the development of disease, these genes seem to be able to operate both in the cis and in the trans position. One of these genes would be situated in the chromosomal portion of the MHS which carries the HLA-D locus. Families with a high incidence of disease would show inheritance according to the cis position of genes, when it can be shown that most of the carriers of the specific disease-associated haplotype are affected by disease, whereas in other families, complementarity between two distinct HLA haplotypes with genes acting in the trans position would result in disease.","PeriodicalId":23105,"journal":{"name":"Tissue antigens","volume":"8 2 1","pages":"131-8"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1399-0039.1976.TB00576.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62840322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-12-11DOI: 10.1111/J.1399-0039.1986.TB01525.X
K. Matsuki, S. Watanabe, Y. Nanzai, M. Nakatate, T. Juji
A variant of the DR2 antigen, DR2 short (DR2S), was investigated in Japanese subjects by using a 9th International Histocompatibility Workshop reagent and locally-obtained alloantisera. A significant positive association between DR2S, Bw67 and TB23 was demonstrated. Haplotype analysis on four Japanese families showed that DR2S was segregated with the A11-Cw7-Bw67-DR2S-DQw1-TB23 haplotype.
使用第9届国际组织相容性研讨会试剂和当地获得的同种抗血清，在日本受试者中研究了DR2抗原的一种变体DR2 short (DR2S)。结果表明，DR2S、Bw67和TB23之间存在显著正相关。单倍型分析表明，DR2S与A11-Cw7-Bw67-DR2S-DQw1-TB23单倍型分离。
{"title":"DR2 short antigen in Japanese. A strong association with Bw67 and TB23.","authors":"K. Matsuki, S. Watanabe, Y. Nanzai, M. Nakatate, T. Juji","doi":"10.1111/J.1399-0039.1986.TB01525.X","DOIUrl":"https://doi.org/10.1111/J.1399-0039.1986.TB01525.X","url":null,"abstract":"A variant of the DR2 antigen, DR2 short (DR2S), was investigated in Japanese subjects by using a 9th International Histocompatibility Workshop reagent and locally-obtained alloantisera. A significant positive association between DR2S, Bw67 and TB23 was demonstrated. Haplotype analysis on four Japanese families showed that DR2S was segregated with the A11-Cw7-Bw67-DR2S-DQw1-TB23 haplotype.","PeriodicalId":23105,"journal":{"name":"Tissue antigens","volume":"27 4 1","pages":"239-43"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1399-0039.1986.TB01525.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62843339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-12-11DOI: 10.1111/J.1399-0039.1980.TB00316.X
T. Kristensen
{"title":"Human histocompatibility testing by T cell-mediated lympholysis: a European standard CML technique. Report from the European CML study group on the Third European CML workshop. Centre d'immunologie INSERM-CNRS. Marseille, December 1979.","authors":"T. Kristensen","doi":"10.1111/J.1399-0039.1980.TB00316.X","DOIUrl":"https://doi.org/10.1111/J.1399-0039.1980.TB00316.X","url":null,"abstract":"","PeriodicalId":23105,"journal":{"name":"Tissue antigens","volume":"16 5 1","pages":"335-67"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1399-0039.1980.TB00316.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62842467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-12-11DOI: 10.1111/J.1399-0039.1975.TB00654.X
F. Grumet, Rose Payne, Junji Konishi, Toru Morr, J. P. Kriss
A group of Japanese were investigated for evidence of an association between Graves' disease and HL-A. Forty-four patients with the disease and 83 normal, unrelated random Japanese and Japanese-American controls were selected for study. The frequency of the W5 antigen among patients (57%) was significantly (P less than .0001) greater than among controls (20%). Of the 34 patients with abnormally elevated serum levels of anti-(thyroid) microsomal (anti-M) auto-antibodies, 56% had the W5 antigen. In contrast, of 48 control individuals tested for anti-M, only seven were seropositive and none (0%) of the seven had the W5 antigen. As expected the HL-A8 antigen was absent from this non-Caucasian population. These data demonstrate that the W5 antigen in Japanese, analogous to the HL-A8 antigen in Caucasians, is associated with Graves' disease but not with anti-M seropositivity in controls. The occurrence of different HL-A antigens in association with the same disease in different ethnic groups requires that the use of a major histocompatibility system antigen as a disease susceptibility marker must be confirmed for each ethnic group under study.
{"title":"HL-A antigens in Japanese Patients with Graves' disease.","authors":"F. Grumet, Rose Payne, Junji Konishi, Toru Morr, J. P. Kriss","doi":"10.1111/J.1399-0039.1975.TB00654.X","DOIUrl":"https://doi.org/10.1111/J.1399-0039.1975.TB00654.X","url":null,"abstract":"A group of Japanese were investigated for evidence of an association between Graves' disease and HL-A. Forty-four patients with the disease and 83 normal, unrelated random Japanese and Japanese-American controls were selected for study. The frequency of the W5 antigen among patients (57%) was significantly (P less than .0001) greater than among controls (20%). Of the 34 patients with abnormally elevated serum levels of anti-(thyroid) microsomal (anti-M) auto-antibodies, 56% had the W5 antigen. In contrast, of 48 control individuals tested for anti-M, only seven were seropositive and none (0%) of the seven had the W5 antigen. As expected the HL-A8 antigen was absent from this non-Caucasian population. These data demonstrate that the W5 antigen in Japanese, analogous to the HL-A8 antigen in Caucasians, is associated with Graves' disease but not with anti-M seropositivity in controls. The occurrence of different HL-A antigens in association with the same disease in different ethnic groups requires that the use of a major histocompatibility system antigen as a disease susceptibility marker must be confirmed for each ethnic group under study.","PeriodicalId":23105,"journal":{"name":"Tissue antigens","volume":"6 5 1","pages":"347-52"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1399-0039.1975.TB00654.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62840141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-12-11DOI: 10.1111/J.1399-0039.1986.TB01534.X
B. Jakobsen, P. Platz, L. Ryder, A. Svejgaard
A new homozygous typing cell (HTC), SK is described. Mixed leukocyte culture (MLC) showed that SK is homozygous for HLA-D"H" (DB6) defined by the HTC, Herluf, and that the HLA-D typing results obtained by Herluf and SK are highly significantly correlated (Kendall's R = 0.46, p = 2.5 X 10(-5)). Both Herluf and SK are also homozygous for a new class II determinant, DN-1, defined by the monoclonal anti-B-lymphocyte antibody, 9w925, developed by Aizawa. The corresponding DN-1 antigen was present in 2.2% of 136 random, unrelated Danes and in all of six unrelated HLA-D"H" positive but in none of 20 HLA-D"H" negative individuals. Thus, there is an absolute and highly significant (p = 4 X 10(-6)) association between the cellularly defined HLA-D"H" determinant and the serologically defined DN-1 antigen, which strongly suggests that HLA-D"H" can now be detected serologically. DN-1 may be identical to DRw12 which is, however, poorly defined. The HTC-donor SK was immunized by pregnancy, and her serum contains anti-HLA-B7, which can easily be absorbed, and anti-B-lymphocyte antibodies which reacted with cells from 87.7% of 536 unrelated Danes. The reaction pattern of this serum is negatively associated with DR3, w6, and w8. This serum may define a new broad, cross-reacting antigen belonging to the same group as DRw52 and DRw53 or DQw1-w3, but it is clearly different from each of these antigens.
描述了一种新的纯合分型细胞(HTC)， SK。混合白细胞培养(MLC)结果显示，SK与HTC、Herluf定义的HLA-D“H”(DB6)为纯合子，且Herluf与SK的HLA-D分型结果高度显著相关(Kendall’s R = 0.46, p = 2.5 × 10(-5))。Herluf和SK对于新的II类决定因子DN-1也是纯合的，该决定因子由Aizawa开发的单克隆抗b淋巴细胞抗体9w925定义。在136名随机无亲缘关系的丹麦人中，有2.2%的人存在相应的DN-1抗原，6名无亲缘关系的HLA-D“H”阳性患者均存在相应的DN-1抗原，但20名HLA-D“H”阴性个体均无相应的DN-1抗原。因此，细胞定义的HLA-D“H”决定因子与血清学定义的DN-1抗原之间存在绝对且高度显著(p = 4 X 10(-6))的关联，这强烈表明HLA-D“H”现在可以通过血清学检测到。DN-1可能与DRw12相同，但DRw12的定义不明确。hc供体SK通过妊娠免疫，其血清中含有易于吸收的抗hla - b7抗体和抗b淋巴细胞抗体，与536名无亲缘关系的丹麦人中87.7%的细胞发生反应。该血清的反应模式与DR3、w6和w8呈负相关。该血清可能定义一种新的广泛的交叉反应抗原，与DRw52和DRw53或DQw1-w3属于同一组，但它与这些抗原明显不同。
{"title":"A new homozygous typing cell with HLA-D\"H\" (DB6) specificity. Evidence that the DN-1 monoclonal antibody 9w925 is specific for the HLA-D\"H\" determinant.","authors":"B. Jakobsen, P. Platz, L. Ryder, A. Svejgaard","doi":"10.1111/J.1399-0039.1986.TB01534.X","DOIUrl":"https://doi.org/10.1111/J.1399-0039.1986.TB01534.X","url":null,"abstract":"A new homozygous typing cell (HTC), SK is described. Mixed leukocyte culture (MLC) showed that SK is homozygous for HLA-D\"H\" (DB6) defined by the HTC, Herluf, and that the HLA-D typing results obtained by Herluf and SK are highly significantly correlated (Kendall's R = 0.46, p = 2.5 X 10(-5)). Both Herluf and SK are also homozygous for a new class II determinant, DN-1, defined by the monoclonal anti-B-lymphocyte antibody, 9w925, developed by Aizawa. The corresponding DN-1 antigen was present in 2.2% of 136 random, unrelated Danes and in all of six unrelated HLA-D\"H\" positive but in none of 20 HLA-D\"H\" negative individuals. Thus, there is an absolute and highly significant (p = 4 X 10(-6)) association between the cellularly defined HLA-D\"H\" determinant and the serologically defined DN-1 antigen, which strongly suggests that HLA-D\"H\" can now be detected serologically. DN-1 may be identical to DRw12 which is, however, poorly defined. The HTC-donor SK was immunized by pregnancy, and her serum contains anti-HLA-B7, which can easily be absorbed, and anti-B-lymphocyte antibodies which reacted with cells from 87.7% of 536 unrelated Danes. The reaction pattern of this serum is negatively associated with DR3, w6, and w8. This serum may define a new broad, cross-reacting antigen belonging to the same group as DRw52 and DRw53 or DQw1-w3, but it is clearly different from each of these antigens.","PeriodicalId":23105,"journal":{"name":"Tissue antigens","volume":"27 5 1","pages":"285-90"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1399-0039.1986.TB01534.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62843353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-12-11DOI: 10.1111/J.1399-0039.1975.TB00657.X
H. Jensen, L. Ryder, L. Nielsen, E. Clausen, F. Jørgensen, H. Jorgensen
{"title":"HLA antigens and glomerulonephritis.","authors":"H. Jensen, L. Ryder, L. Nielsen, E. Clausen, F. Jørgensen, H. Jorgensen","doi":"10.1111/J.1399-0039.1975.TB00657.X","DOIUrl":"https://doi.org/10.1111/J.1399-0039.1975.TB00657.X","url":null,"abstract":"","PeriodicalId":23105,"journal":{"name":"Tissue antigens","volume":"6 5 1","pages":"268-9"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1399-0039.1975.TB00657.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62840152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-12-11DOI: 10.1111/J.1399-0039.1986.TB00499.X
B. Larsen, C. Thompson, A. Kwan, N. Farid
We examined HLA association with differentiated thyroid carcinoma in 45 patients from Newfoundland. No association was found. This finding contrasts with the description of an association of thyroid cancer with HLA-DR1 in Italy and Hungary (iodide deficient areas) and with DR7 in the American mid-west. We suggest that iodide deficiency predisposes DR1 + individuals to thyroid cancer and that this risk is negated by iodide sufficiency unless some other risk factor supervenes.
{"title":"Lack of association of HLA with thyroid cancer. An effect of iodine sufficiency and safe environment?","authors":"B. Larsen, C. Thompson, A. Kwan, N. Farid","doi":"10.1111/J.1399-0039.1986.TB00499.X","DOIUrl":"https://doi.org/10.1111/J.1399-0039.1986.TB00499.X","url":null,"abstract":"We examined HLA association with differentiated thyroid carcinoma in 45 patients from Newfoundland. No association was found. This finding contrasts with the description of an association of thyroid cancer with HLA-DR1 in Italy and Hungary (iodide deficient areas) and with DR7 in the American mid-west. We suggest that iodide deficiency predisposes DR1 + individuals to thyroid cancer and that this risk is negated by iodide sufficiency unless some other risk factor supervenes.","PeriodicalId":23105,"journal":{"name":"Tissue antigens","volume":"28 5 1","pages":"298-300"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1399-0039.1986.TB00499.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62843273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-12-11DOI: 10.1111/J.1399-0039.1987.TB01542.X
D. Francis, J. Batchelor, W. Mcdonald, I. Dodi, S. Hing, J. Hern, A. Downie
Fourteen multiplex MS families, 9 single-case MS families and 11 normal families from the Grampian region of North-East Scotland were studied. The prevalence rate of MS for individuals in multiplex families was calculated at 809/100,000; 4.5 times the prevalence rate for the general population in this region. The distribution of shared haplotypes in 12 affected and 19 unaffected sib-pair comparisons did not differ significantly from that expected by chance. Furthermore there was no evidence that homozygosity of a particular HLA gene was required for increased susceptibility to the disease. HLA-B7, C4A3, C4B1, BfS, HLA-DR2, HLA-DQw1 was the commonest haplotype accounting for 18.9% and 24.2% of parental haplotypes from multiplex and single-case families, respectively, compared with 2.3% of parental haplotypes from control families (p less than 0.05 and p less than 0.01, respectively). No significant differences were observed in the frequencies of complement gene polymorphisms (Factor B and C4). The data suggests that a MS susceptibility gene exists, in the HLA complex, and is in closest linkage disequilibrium with the HLA-D region; although other factors, environmental and/or independent genetic loci, may have an important influence.
{"title":"HLA genetic determinants in familial MS. A study from the Grampian region of Scotland.","authors":"D. Francis, J. Batchelor, W. Mcdonald, I. Dodi, S. Hing, J. Hern, A. Downie","doi":"10.1111/J.1399-0039.1987.TB01542.X","DOIUrl":"https://doi.org/10.1111/J.1399-0039.1987.TB01542.X","url":null,"abstract":"Fourteen multiplex MS families, 9 single-case MS families and 11 normal families from the Grampian region of North-East Scotland were studied. The prevalence rate of MS for individuals in multiplex families was calculated at 809/100,000; 4.5 times the prevalence rate for the general population in this region. The distribution of shared haplotypes in 12 affected and 19 unaffected sib-pair comparisons did not differ significantly from that expected by chance. Furthermore there was no evidence that homozygosity of a particular HLA gene was required for increased susceptibility to the disease. HLA-B7, C4A3, C4B1, BfS, HLA-DR2, HLA-DQw1 was the commonest haplotype accounting for 18.9% and 24.2% of parental haplotypes from multiplex and single-case families, respectively, compared with 2.3% of parental haplotypes from control families (p less than 0.05 and p less than 0.01, respectively). No significant differences were observed in the frequencies of complement gene polymorphisms (Factor B and C4). The data suggests that a MS susceptibility gene exists, in the HLA complex, and is in closest linkage disequilibrium with the HLA-D region; although other factors, environmental and/or independent genetic loci, may have an important influence.","PeriodicalId":23105,"journal":{"name":"Tissue antigens","volume":"29 1 1","pages":"7-12"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1399-0039.1987.TB01542.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62842904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2008-12-11DOI: 10.1111/J.1399-0039.1983.TB00379.X
G. Groenewegen, W. Buurman, C. J. van der Linden, G. Jeunhomme, G. Kootstra
A method is described which permits culture of both arterial and venous canine endothelial cells. Cell-mediated cytotoxicity against cultured endothelial cells has been studied. A 51Cr-release assay was used to detect CTL generated in MLC. Both arterial and venous endothelial cells are lysed by CTL specifically. Cold target inhibition experiments have been performed to analyse the CTL-recognized antigens on arterial and venous endothelial cells. Different antigens are recognized by CTL on venous endothelial cells and PHA-blasts; it is possible that CTL recognize venous endothelial cells through class II antigens or E-M antigens. Arterial endothelial cells and PHA-blasts share CTL-recognized antigens.
{"title":"Cellular cytotoxicity against canine endothelial cells. Analysis of determinants recognized by CTL.","authors":"G. Groenewegen, W. Buurman, C. J. van der Linden, G. Jeunhomme, G. Kootstra","doi":"10.1111/J.1399-0039.1983.TB00379.X","DOIUrl":"https://doi.org/10.1111/J.1399-0039.1983.TB00379.X","url":null,"abstract":"A method is described which permits culture of both arterial and venous canine endothelial cells. Cell-mediated cytotoxicity against cultured endothelial cells has been studied. A 51Cr-release assay was used to detect CTL generated in MLC. Both arterial and venous endothelial cells are lysed by CTL specifically. Cold target inhibition experiments have been performed to analyse the CTL-recognized antigens on arterial and venous endothelial cells. Different antigens are recognized by CTL on venous endothelial cells and PHA-blasts; it is possible that CTL recognize venous endothelial cells through class II antigens or E-M antigens. Arterial endothelial cells and PHA-blasts share CTL-recognized antigens.","PeriodicalId":23105,"journal":{"name":"Tissue antigens","volume":"21 2 1","pages":"114-28"},"PeriodicalIF":0.0,"publicationDate":"2008-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1399-0039.1983.TB00379.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"62843096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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