Transplantologiya. The Russian Journal of Transplantation最新文献

{"title":"PHENOMENON OF DEMIKHOV. Heart transplantation in experiment and clinical cases in the USSR and abroad (1968–1972). The Second in the USSR heart transplantation in a Clinic (Solovyev G.M., June 10, 1971)","authors":"S. Glyantsev, Yu. A. Shabunts, M. I. Chernenko","doi":"10.23873/2074-0506-2022-14-3-371-390","DOIUrl":"https://doi.org/10.23873/2074-0506-2022-14-3-371-390","url":null,"abstract":"The article presents materials of Soviet and foreign medical literature of 1968–1972 devoted to heart transplantation in experiment and clinical practice. It is shown that in the USSR after unsuccessful heart transplantation performed by A.A. Vishnevsky on November 4, 1968, experimental studies on isolated heart preservation in order to preserve its viability were conducted; models of orthotopic and heterotopic heart transplantation on large and small animals were created; morphological, physiological, biochemical and immunological changes in the transplanted heart were studied. The second heart transplantation in this country was performed by G.M. Solovyev on June 10, 1971, but it was also unsuccessful. It’s remarkable that the 20-year experience of V.P. Demikhov in experimental heart transplantation was used only partially: a number of surgeons applied the cardiopulmonary complex isolated according to V.P. Demikhov's technique for biological heart preservation in experimental animals. At the same time after the successful heart transplantation performed by C. Barnard on December 3, 1967, the world boom of clinical transplantation began. In 1969, 101 such operations were performed. For example, D. Cooley performed them on 21 patients and on another one performed two surgeries. There were observations that patients operated on in 1968 lived 800–900 days or more. The best results were shown by R. Lower, M. DeBakey and N. Shumway. However, the vast majority of heart transplants resulted in lethal outcomes in the immediate or distant postoperative period. This led to the fact that the euphoria of successful transplants gradually began to diminish: in 1969 only 47 operations were performed, and in 1970–1971 only 17 surgeries per year.","PeriodicalId":23229,"journal":{"name":"Transplantologiya. The Russian Journal of Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80348068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Easy to say, hard to do. To the anniversary of two fundamental discoveries. Part 1","authors":"A. Y. Anisimov","doi":"10.23873/2074-0506-2022-14-3-357-370","DOIUrl":"https://doi.org/10.23873/2074-0506-2022-14-3-357-370","url":null,"abstract":"On the eve of the anniversaries of two historical events: the 145th anniversary of the experiments in which Eck's fistula was performed and the 55th anniversary of the successful clinical approbation of the selective distal splenorenal anastomosis, a retrospective analysis of the key historical stages in the development of portal hypertension surgery from the first attempts to describe the anatomy of the hepatic vascular system, explaining its purpose in the body, the development of direct portocaval anastomosis, the widespread use of selective splenorenal anastomoses, to the Transjugular Intrahepatic Portosystemic Shunt procedure and orthotopic liver transplantation. At the same time, the emphasis is focused on the most colorful characters of researchers and clinicians who passed this path. The expediency of an integrated approach in solving the problems of portal hypertension with the development of both fundamental and applied clinical and organizational aspects is substantiated. It is shown that the discoveries born from the generalization of the results achieved by numerous researchers have contributed to a better understanding of this field of medicine, have become a solid foundation for what we have today and are a reliable platform for a successful start into the future.","PeriodicalId":23229,"journal":{"name":"Transplantologiya. The Russian Journal of Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87196724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Available methods to enhance regenerative potential of plastic materials for bone defects replacement in orthopedics. Part 3. Use of autologous human red bone marrow","authors":"A. Fain, A. Vaza, S. Gnetetskiy, K. I. Skuratovskaya, V. B. Bondarev, Yu. A. Bogolyubskiy, R. Titov, A. Sergeev","doi":"10.23873/2074-0506-2022-14-3-344-356","DOIUrl":"https://doi.org/10.23873/2074-0506-2022-14-3-344-356","url":null,"abstract":"The previous two articles described the use of platelet-rich plasma and platelet lysate. This part of the literature review examines the mechanism of red bone marrow action, indications and contraindications for its use. The results of treatment for delayed consolidation of bone fractures are also described. Hematopoietic stem cells give rise to all cellular components of the circulating blood, such as red blood cells, lymphocytes, neutrophils, and platelets. The most rational way to stimulate bone regeneration is to use the patient's own biological material. The aim of this article is to summarize the results of treatment using autologus bone marrow to improve bone regenerative potential in orthopaedics.","PeriodicalId":23229,"journal":{"name":"Transplantologiya. The Russian Journal of Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85019019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elimination of factors contributing to the development of colo-esophageal anastomotic dehiscence following retrosternal colonic esophagoplasty performed for esophageal atresia correction","authors":"A. Kivva, M. Chepurnoy, B. M. Belik, Y. Tyshlek, M. Shtilman, A. A. Kivva, I. R. Chumburidze","doi":"10.23873/2074-0506-2022-14-3-322-330","DOIUrl":"https://doi.org/10.23873/2074-0506-2022-14-3-322-330","url":null,"abstract":"Aim. Development of methodology that would allow surgeons to decrease the risk of colo-esophageal anastomotic dehiscence following esophagoplasty through prevention of colonic transplant compression in the retrosternal tunnel and surgery facilitation.Material and methods. Ultrasound examination was carried out on 43 infants (22 boys and 21 girls) without sternal pathologies. The dorsal sternal angle and thickness of the upper and lower parts of the sternal manubrium were evaluated. The prominence and structure of muscular arrangement in various parts of the dorsal side of the sternal manubrium were examined in 15 infant patients. The results of treatment based on the method developed in accordance with the RF invention patent No. 2552095 were analyzed for infant patients (8 boys and 7 girls) suffering from esophageal atresia. 9 patients had fenestration of the sternal manubrium performed across its entire length, 6 children – in its upper segment only. X-ray control was used to detect colonic transplant compression. Significant difference between the variables in question in the examined groups was evaluated using the Mann-Whitney U test (M-W) for data measured on an ordinal scale.Results. The upper part of the sternal manubrium (above the attachment of the first rib) was found to exert the highest pressure on the transplant as it is statistically significantly thicker (M-W=4.44; p<0.01), being covered with a more prominent muscular layer (M-W=6.71; p<0.001) over a larger area (M-W=4.42; p<0.01) and considerably reclined. In infant age, the dorsal sternal angle is 164.9±0.8 degrees. Its value was consistently (M-W=2.66; p<0.01) higher in the girls' group with significant individual variations. Based on the collected data, an original technique was developed for individual approach to the resection of the manubrium sterni applied during retrosternal colonic esophagoplasty in 15 patients with esophageal atresia. No signs of colo-esophageal anastomotic dehiscence or transplant compression were revealed in any of those cases.Conclusion. The suggested method allows surgeons to assess in every case the narrowness of retrosternal space and individually select the scope of sternal manubrium resection to eliminate important factors contributing to the development of colo-esophageal anastomotic dehiscence after retrosternal esophagoplasty in cases of esophageal atresia thus facilitating the surgery and preventing transplant compression in the retrosternal tunnel.","PeriodicalId":23229,"journal":{"name":"Transplantologiya. The Russian Journal of Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77708346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Burdened urological history as a risk factor in kidney transplantation for older age group recipients","authors":"B. Z. Khubutiya, M. Khubutiya, A. Evseev, A. V. Babkina","doi":"10.23873/2074-0506-2022-14-3-331-343","DOIUrl":"https://doi.org/10.23873/2074-0506-2022-14-3-331-343","url":null,"abstract":"Introduction. Chronic renal failure develops more often in elderly patients after previous repeated urological operations. Urological diseases are an important additional factor that multiply complicates kidney transplantation due to the need for preliminary correction of existing disorders. Not to mention the fact that the recipient's advanced age contributes to the risk of developing graft dysfunction due to the presence of concomitant diseases that worsen the immediate and long-term results of transplantation.Material and methods. We carried out the analysis of clinical observations and studies performed in 124 patients who underwent cadaveric renal allotransplantation at the N.V. Sklifosovsky Research Institute for Emergency Medicine, including from suboptimal donors. Of these, 69 (55.6%) recipients older than 60 years were included in the main group (group 1), and 55 recipients younger than 60 years (44.4%) – in the comparison group (group 2). From the patients of group 1, patients with urological diseases that led to the development of terminal chronic renal failure, or with previously undergone surgeries for urological diseases (subgroup 1A, 43 patients) were isolated. The remaining 26 patients who did not have urological diseases were put in subgroup 1B.Results. The analysis showed that in the early postoperative period in the recipients of group 1, complications developed in almost all the patients (80 complications in 64 of 69 patients, that is, 92.8% of patients in this group), whereas in group 2, complications developed only in 30.9% of patients (27 complications in 17 of 55 patients). At the same time, on average, 1.2 complications per 1 patient were noted in group 1, and 0.5 complications per 1 patient in group 2, a significant part of the complications occurred in the subgroup of patients with a burdened urological history (subgroup 1A). Late complications also developed statistically significantly more often (almost 2 times) in recipients with a burdened urological history.Conclusions. Complications of the early and late postoperative period develop statistically significantly more often in elderly recipients. The presence of a burdened urological history in elderly recipients significantly increases the risk of early and late postoperative complications.","PeriodicalId":23229,"journal":{"name":"Transplantologiya. The Russian Journal of Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74485598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of an optimal cryoprotectant for long-term storage of human tendon allografts","authors":"A. A. Budaev, N. Borovkova, A. M. Fayn, M. Makarov, M. V. Storozheva, Y. Andreev, A. Mironov","doi":"10.23873/2074-0506-2022-14-3-312-321","DOIUrl":"https://doi.org/10.23873/2074-0506-2022-14-3-312-321","url":null,"abstract":"Introduction. Tendon grafts are widely demanded in reconstructive plastic surgery. Allogeneic tendons potentially have a number of advantages. However, the method of long-term storage of allogeneic tendons has not been optimized to date.Aim. Selection of an optimal cryoprotectant for the storage of human tendon allografts at ultra-low temperatures, which allows preserving the native tissue structure.Material and methods. We studied M. tibialis anterior tendon grafts taken from tissue donors. Endocellular/penetrating cryoprotectants (dimethyl sulfoxide, polyethylene glycol-400, glycerol) and exocellular/non-penetrating cryoprotectants (glucose solution, albumin solution) were used in the cryopreservation process. Tendon mechanical properties were evaluated using rupture and stretching-shear test, the general morphology of tendons, topography, dense and integrity of collagen fibers, preservation of cellular elements were microscopically evaluated.Results. Histological analysis showed that the safety of collagen and elastin fibers differed depending on the cryoprotectant used. At the same time, micro-fractures of collagen fibers were microscopically detected in all the experiments. In the presence of dimethyl sulfoxide, polyethylene glycol-400 and their combination the structure of collagen fibers and cells did not undergo visible changes compared to the control, whereas in all the experiments with non-penetrating cryoprotectants the topography and orientation of the fibers were clearly disturbed, deformation of many cells in the tendons was also observed.Conclusions. Cryoprotectants based on dimethyl sulfoxide, polyethylene glycol and their combinations allowed us to preserve the structural integrity of allogeneic tendons. Non-penetrating cryoprotectants did not effectively preserve the integrity of collagen fibers and cells in the tendons and cannot be recommended for cryopreservation.","PeriodicalId":23229,"journal":{"name":"Transplantologiya. The Russian Journal of Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83413067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The place of glucosylated enteral solution in the correction of hemorheological abnormalities in acute poisoning by psychopharmacological drugs","authors":"M. M. Potskhveriya, K. K. Ilyashenko, M. V. Belova, A. Simonova, E. E. Bitkova","doi":"10.23873/2074-0506-2022-14-3-301-311","DOIUrl":"https://doi.org/10.23873/2074-0506-2022-14-3-301-311","url":null,"abstract":"Introduction. In acute exogenous poisoning, hemorheological abnormalities are observed. Various extracorporeal, physico-chemical methods are used to correct them. There is an opinion that the enteral route of administration of corrective agents may be a more physiological way to restore homeostatic imbalances.Aim. To conduct a comparative assessment of the effect of glucosylated enteral solution and standard infusion therapy on hemorheological abnormalities in acute poisoning by psychopharmacological drugs.Material and methods. Patients with acute poisoning by psychopharmacological drugs who were treated at the N.V. Sklifosovsky Research Institute for Emergency Medicine in 2017–2021 were examined. Of these, 23 people, in whose treatment the enteral correction program was used, made up the study group, and 22 patients (the comparison group) underwent a standard set of therapeutic measures. Indicators of hemorheological status were examined on the 1st, 3rd and 5th days against the background of ongoing therapy. Statistical data analysis was carried out using the Statistica 10 software package (StatSoft, Inc., USA).Results. The use of a glucosylated enteral solution led to a reduction in plasma viscosity under normal hematocrit conditions at all follow-up periods. In patients of both groups, there was a decrease in blood viscoelasticity under conditions of high shear potential at all stages of the study, which indicates impaired red blood cell deformability. This process was more pronounced in individuals of the comparison group. The conducted studies have shown that the use of infusion therapy and glucosylated enteral solution in the early stages of acute poisoning by psychopharmacological drugs generally has a unidirectional positive effect on hemorheological indicators, contributing to the stabilization of blood circulation. At the same time, the effect of glucosylated enteral solution therapy is faster and more pronounced.Conclusions. The use of glucosylated enteral solution and infusion therapy as a supportive treatment in the early period of acute poisoning by psychopharmacological drugs in most cases has a unidirectional effect on hemorheological parameters. In cases of glucosylated enteral solution therapy, there was an outrunning positive dynamics on the part of the majority of the studied hemorheological parameters. Glucosylated enteral solution can be the method of choice as a maintenance therapy after the end of detoxification process for acute poisoning by psychopharmacological drugs.","PeriodicalId":23229,"journal":{"name":"Transplantologiya. The Russian Journal of Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85063306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of calcineurin inhibitors in the progression of hepatocellular carcinoma after liver transplantation","authors":"O. Olisov, M. Novruzbekov, V. Gulyaev, K. Lutsyk","doi":"10.23873/2074-0506-2022-14-3-292-300","DOIUrl":"https://doi.org/10.23873/2074-0506-2022-14-3-292-300","url":null,"abstract":"Introduction. Orthotopic liver transplantation is the most radical method of treatment of hepatocellular carcinoma. The high recurrence rate limits the use of transplantation in patients with hepatocellular cancer. Immunosuppressive therapy may affect the frequency of oncoprogression after liver transplantationAim. To evaluate the role of immunosuppressive therapy in the postoperative progression of hepatocellular cancer in patients after liver transplantationMaterial and methods. The recurrence rate of hepatocellular cancer and tumor free survival in 104 patients after liver transplantation were analyzed. To evaluate the effect of the immunosuppression main component concentration on the postoperative progression of hepatocellular carcinoma, we studied the mean baseline concentration (C0) for the entire follow-up period for patients with a tumor-free period and the mean baseline concentration for patients with hepatocellular carcinoma progression, in whom only the duration of the tumor-free period was studied. According to the degree of tumor lesion, patients were distributed in accordance with the Milan criteria (based on the results of a pathologic and morphological examination of the recipient's explanted liver.Results. The values of the baseline blood level of tacrolimus>6.0 ng/ml and cyclosporine A>100 ng/ml is associated with a high rate of progression of hepatocellular cancer. Reducing the load of calcineurin inhibitors can reduce the incidence of cancer progression by at least 2 times. The values of 1-, 3- and 5-year relapse-free survival in patients with advanced cancer and low figures of the baseline blood level of calcineurin inhibitor are 82%, 70% and 70%, respectively.Conclusion. Minimization of immunosuppression is of crucial importance in the prevention of posttransplant progression of hepatocellular cancer, especially among patients with its common form.","PeriodicalId":23229,"journal":{"name":"Transplantologiya. The Russian Journal of Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86315672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic consequences of noncompliant behavior of patients with chronic renal failure and ways of its correction","authors":"Y. Lysenko, O. Mikita, A. Pinchuk, M. Khubutiya","doi":"10.23873/2074-0506-2022-14-3-278-291","DOIUrl":"https://doi.org/10.23873/2074-0506-2022-14-3-278-291","url":null,"abstract":"Introduction. Kidney transplantation is the main high-tech type of medical care aimed at saving the life of a patient with chronic kidney disease in the terminal stage of the disease. The amount of economic costs for the treatment of patients with chronic renal failure depends on the results of transplantation: the calculation for 1 patient with a favorable outcome is 1,665,110.19 rubles, with an unfavorable outcome – 2,932,078.07 rubles. Noncompliant behavior (non-compliance with doctors' recommendations) directly affects the effectiveness and outcome of transplantation, as well as its economic component. In order to minimize the factors affecting transplant rejection after surgery due to patient noncompliant behavior, it is necessary to assess adherence to treatment at the stages before and after transplantation.Aim. To assess the adherence to treatment of patients with chronic renal failure of the N.V. Sklifosovsky Research Institute for Emergency Medicine at all stages of kidney transplantation in order to identify risk groups for noncompliant behavior.Material and methods. The study was conducted on the basis of the Department of Kidney and Pancreas Transplantation of the N.V. Sklifosovsky Research Institute for Emergency Medicine. 3 groups of patients with a diagnosis of \"Chronic renal failure\" were examined: 1. 48 patients before kidney transplantation who were on the waiting list. The average age of patients: 48.06±10.21 years. Gender composition of the group: 40% men, 60% women. 2. 62 patients a month after kidney transplantation. The average age of patients: 49.49±11.04 years. Gender composition of the group: 41% men, 59% women. 3. 22 patients more than 1 month after kidney transplantation. The average age of patients: 43.39±12.29 years. Gender composition of the group: 41% men, 59% women.The assessment of the level of adherence to treatment was carried out using the following psychodiagnostic techniques: \"Quantitative assessment of adherence to treatment\", \"Social significance of the disease\" and \"Compliance level\".Results. The data obtained by us demonstrate the highest level of adherence to treatment in the waiting list group (90.67±18.91 points), in the group a month after transplantation this level decreases (71.29±22.70 points), the lowest indicators (65.73±23.47 points) in the group a few months after kidney transplantation.Conclusion. Noncompliant behavior of patients can affect the treatment outcomes. According to our study, patients on the waiting list have the highest rates in terms of adherence to treatment, the group of patients a month after transplantation is characterized by a decrease in all indicators, and the group a few months after kidney transplantation is characterized by the lowest parameters. According to the dynamics of changes in the level of adherence to treatment in the groups we examined, it is recommended to prevent noncompliant behavior in order to increase the treatment effectiveness and minimize financi","PeriodicalId":23229,"journal":{"name":"Transplantologiya. The Russian Journal of Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83287930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of pretransplant and de novo anti-HLA antibody detection after simultaneous pancreas-kidney transplantation","authors":"I. Dmitriev, N. Borovkova, S. Shchelykalina, N. V. Doronina, N. Zhuravel, A. Pinchuk","doi":"10.23873/2074-0506-2022-14-3-254-264","DOIUrl":"https://doi.org/10.23873/2074-0506-2022-14-3-254-264","url":null,"abstract":"Introduction. Nowadays, there are few studies concerning assessment of the clinical significance of determining the level of pretransplant and de novo anti-HLA antibodies in patients after simultaneous pancreas-kidney transplantation.Aim. The study of the incidence, timing of formation and specificity of pretransplant and de novo anti-HLA antibodies in patients after simultaneous pancreas-kidney transplantation.Material and methods. We conducted a prospective and retrospective research to study the incidence, timing of formation and specificity of pretransplant and de novo anti-HLA antibodies in 55 patients after simultaneous pancreas-kidney transplantation performed at the N.V. Sklifosovsky Research Institute for Emergency Medicine from 2008 to 2022.Results. There were 4 patients with preformed anti-HLA antibodies (7%). The formation of de novo anti-HLA antibodies after simultaneous pancreas-kidney transplantation was observed in 17 patients (31%). There were 5 patients with antiHLA class I, 3 patients with anti-HLA class II, 3 patients with anti-HLA class I and II, 5 patients with anti-MICA and 1 patient with both classes of anti-HLA and anti-MICA. The formation of de novo anti-HLA antibodies significantly increased the incidence of acute rejection (47% compared with 13%, p=0.014).Conclusion. The frequency of pretransplant and de novo anti-HLA antibody detection in the recipients at our Center is comparable to published data from other transplant centers. We obtained evidence that the formation of de novo antiHLA antibodies increases the incidence of acute rejection after simultaneous pancreas-kidney transplantation.","PeriodicalId":23229,"journal":{"name":"Transplantologiya. The Russian Journal of Transplantation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86127184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}