Pub Date : 2021-06-25DOI: 10.51607/22331360.2021.70.2.169
Enida Članjak – Kudra, Neira Fazlović, D. Alagić, M. Smajlović, K. Čaklovica, Ahmed Smajlović
The increase of global fish consumption brings with it the problem of fish mislabeling, which leads to economic losses for consumers or may even result in consumers’ health risk. Thus, continuous development of methods used in identification of exact fish species is essential. There are numerous modern identification methods, which use DNA or specific fish proteins as biomarkers, but they all come with various benefits and drawbacks. Protein-based methods include electrophoretic methods, ELISA, HPLC and MALDI-ToF Mass Spectrometry, while the methods that use DNA as a biomarker include PCR-RFLP, SSCP, qPCR, RAPD, FINS, DNA microarray, DNA barcoding, pyrosequencing and metabarcoding. There is a continuous development of new or improved methods. Most of the methods display a much better performance when analyzing raw or lightly processed fish, such as chilled or frozen, while heat treatment often leads to changes in the biomarker molecules, making the species identification difficult. The ideal method does not yet exist, but methods such as DNA barcoding, MALDI-ToF Mass Spectrometry and metabarcoding are the ones that show most potential.
{"title":"An overview of most commonly used methods for detection of fish mislabeling","authors":"Enida Članjak – Kudra, Neira Fazlović, D. Alagić, M. Smajlović, K. Čaklovica, Ahmed Smajlović","doi":"10.51607/22331360.2021.70.2.169","DOIUrl":"https://doi.org/10.51607/22331360.2021.70.2.169","url":null,"abstract":"The increase of global fish consumption brings with it the problem of fish mislabeling, which leads to economic losses for consumers or may even result in consumers’ health risk. Thus, continuous development of methods used in identification of exact fish species is essential. There are numerous modern identification methods, which use DNA or specific fish proteins as biomarkers, but they all come with various benefits and drawbacks. Protein-based methods include electrophoretic methods, ELISA, HPLC and MALDI-ToF Mass Spectrometry, while the methods that use DNA as a biomarker include PCR-RFLP, SSCP, qPCR, RAPD, FINS, DNA microarray, DNA barcoding, pyrosequencing and metabarcoding. There is a continuous development of new or improved methods. Most of the methods display a much better performance when analyzing raw or lightly processed fish, such as chilled or frozen, while heat treatment often leads to changes in the biomarker molecules, making the species identification difficult. The ideal method does not yet exist, but methods such as DNA barcoding, MALDI-ToF Mass Spectrometry and metabarcoding are the ones that show most potential.","PeriodicalId":23552,"journal":{"name":"Veterinaria","volume":"587 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81685361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-25DOI: 10.51607/22331360.2021.70.2.185
I. Ergin, Sumeyye Sainkaplan, O. Şenel
Chronic superficial keratitis (CSK), also called pannus or Uberreiter’s syndrome is a chronic progressive corneal disease in dogs. This autoimmune disease of unknown etiology and pathogenesis is characterized by infiltration of immune cells to corneal epithelium and stromal layers. The aim of this study was to evaluate occurrence and appearance of clinical symptoms of Uberreiter’s syndrome and reveal treatment results in dogs. Fifty-five dogs were assessed in the study. Vascularization and pigmentation in the temporal, nasal, superior and/or inferior quadrant of the cornea were observed in clinical examination of dogs with different grades of ocular discharge, vision loss and conjunctival hyperemia. CSK was most commonly seen in German shepherd breed (n=42). Cyclosporin, corticosteroid, hyaluronic acid and broad-spectrum antibiotic eye drops were administered as medical treatment. As a result, it was observed that the progress of pigmentation was largely slowed and regressed in some cases when two basic immunosuppressive drugs used for CSK were administered regularly in the eyes. Still, many of them that have been followed are not blind, and these animals continue to maintain their lives comfortably.
{"title":"Clinical assessment of chronic superficial keratitis (Überreiter’s syndrome) in dogs: A retrospective study (2012-2019)","authors":"I. Ergin, Sumeyye Sainkaplan, O. Şenel","doi":"10.51607/22331360.2021.70.2.185","DOIUrl":"https://doi.org/10.51607/22331360.2021.70.2.185","url":null,"abstract":"Chronic superficial keratitis (CSK), also called pannus or Uberreiter’s syndrome is a chronic progressive corneal disease in dogs. This autoimmune disease of unknown etiology and pathogenesis is characterized by infiltration of immune cells to corneal epithelium and stromal layers. The aim of this study was to evaluate occurrence and appearance of clinical symptoms of Uberreiter’s syndrome and reveal treatment results in dogs. Fifty-five dogs were assessed in the study. Vascularization and pigmentation in the temporal, nasal, superior and/or inferior quadrant of the cornea were observed in clinical examination of dogs with different grades of ocular discharge, vision loss and conjunctival hyperemia. CSK was most commonly seen in German shepherd breed (n=42). Cyclosporin, corticosteroid, hyaluronic acid and broad-spectrum antibiotic eye drops were administered as medical treatment. As a result, it was observed that the progress of pigmentation was largely slowed and regressed in some cases when two basic immunosuppressive drugs used for CSK were administered regularly in the eyes. Still, many of them that have been followed are not blind, and these animals continue to maintain their lives comfortably.","PeriodicalId":23552,"journal":{"name":"Veterinaria","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77780001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-25DOI: 10.51607/22331360.2021.70.2.245
N. Belaid-Gater, Saliha Gaoua, Si Ammar KADI
The aim of this study was to evaluate the effect of two medicinal plants, Fenugreek and sagebrush, on the performance (growth, slaughter parameters and meat quality, biochemical and blood lipid parameters, bacteriological, virological and parasitological analyses) of broiler chickens. 300 Cobb 500 broiler chicks were used. On the 11th day of age, they were weighed and distributed in three lots of 100 chicks at a rate of 04 replicates of 25 chicks. The chickens in the control lot (T) consumed pure water, those in the two experimental lots water, one with Fenugreek infusion (F) and the other with sagebrush infusion (A). The experiment finished when the chickens reached 6 weeks of age, i.e. 42 days. Supplementation with Fenugreek and sagebrush infusions significantly affected final body weight, weight gain, feed intake, feed conversion rate and water consumption. However, no significant difference was noted for slaughter parameters except for liver weight which was higher in the sagebrush lot. The blood biochemical parameters of the chickens in the two experimental lots were not affected. In addition, the meat of the chickens in the sagebrush and Control lots was noted to be very tender and juicier than the meat of the chickens in the Fenugreek lot. Mortality was significantly similar in the two experimental lots and the control lot.
{"title":"Effect of sagebrush (Artemisia herba-alba Asso) and Fenugreek (Trigonella foenum-graecum L.) on growth performances, some biochemical traits and carcass qualities for broiler chickens","authors":"N. Belaid-Gater, Saliha Gaoua, Si Ammar KADI","doi":"10.51607/22331360.2021.70.2.245","DOIUrl":"https://doi.org/10.51607/22331360.2021.70.2.245","url":null,"abstract":"The aim of this study was to evaluate the effect of two medicinal plants, Fenugreek and sagebrush, on the performance (growth, slaughter parameters and meat quality, biochemical and blood lipid parameters, bacteriological, virological and parasitological analyses) of broiler chickens. 300 Cobb 500 broiler chicks were used. On the 11th day of age, they were weighed and distributed in three lots of 100 chicks at a rate of 04 replicates of 25 chicks. The chickens in the control lot (T) consumed pure water, those in the two experimental lots water, one with Fenugreek infusion (F) and the other with sagebrush infusion (A). The experiment finished when the chickens reached 6 weeks of age, i.e. 42 days. Supplementation with Fenugreek and sagebrush infusions significantly affected final body weight, weight gain, feed intake, feed conversion rate and water consumption. However, no significant difference was noted for slaughter parameters except for liver weight which was higher in the sagebrush lot. The blood biochemical parameters of the chickens in the two experimental lots were not affected. In addition, the meat of the chickens in the sagebrush and Control lots was noted to be very tender and juicier than the meat of the chickens in the Fenugreek lot. Mortality was significantly similar in the two experimental lots and the control lot.","PeriodicalId":23552,"journal":{"name":"Veterinaria","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90512029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-25DOI: 10.51607/22331360.2021.70.2.229
Ruhsar Ekiz, Y. Demiraslan
The aim of this study is to evaluate the cadaver embalming effects of the honey-based solutions used as an alternative to formaldehyde which is extremely hazardous for human health. In the study, 42 sheep hearts were used. Tissue and swap samples were taken from different honey solution groups on the 3rd, 75th, 150th, and 225th day to perform histological, microbiological, texture and color analyses. In the macroscopic examination, there was a more significant hardness in the hearts kept in the solution I in A and B groups than the solutions II and III. General fixation was found to be positive in the histological analysis. However, the histological structure became deformed later. The microbiological analysis showed no bacterial or mycotic growth. Moreover, according to the comparative results of the hardness scale applied to the groups, the heart tissues which were kept in AI, BII, and BIIIsolutions on the 3rd day, in AII and BIII solutions on the 150th day, in BIII solutions on the 225th day, in AIII on all days werestatistically found to be similar to the fresh heart tissue. Based on the results of the color analysis, the heart tissues of the experimental groups were not statistically similar to the fresh heart tissue in within-group and between-group comparisons of L value. Consequently, it is thought that the positive effect of honey solutions on the sheep heart tissue should be evaluated in organs with different parenchyma tissue and properties and whole cadaver.
{"title":"Investigation of cadaver preservative properties of solution obtained with a mixture of honey and ethyl alcohol in different proportions: A pilot study on sheep heart","authors":"Ruhsar Ekiz, Y. Demiraslan","doi":"10.51607/22331360.2021.70.2.229","DOIUrl":"https://doi.org/10.51607/22331360.2021.70.2.229","url":null,"abstract":"The aim of this study is to evaluate the cadaver embalming effects of the honey-based solutions used as an alternative to formaldehyde which is extremely hazardous for human health. In the study, 42 sheep hearts were used. Tissue and swap samples were taken from different honey solution groups on the 3rd, 75th, 150th, and 225th day to perform histological, microbiological, texture and color analyses. In the macroscopic examination, there was a more significant hardness in the hearts kept in the solution I in A and B groups than the solutions II and III. General fixation was found to be positive in the histological analysis. However, the histological structure became deformed later. The microbiological analysis showed no bacterial or mycotic growth. Moreover, according to the comparative results of the hardness scale applied to the groups, the heart tissues which were kept in AI, BII, and BIIIsolutions on the 3rd day, in AII and BIII solutions on the 150th day, in BIII solutions on the 225th day, in AIII on all days werestatistically found to be similar to the fresh heart tissue. Based on the results of the color analysis, the heart tissues of the experimental groups were not statistically similar to the fresh heart tissue in within-group and between-group comparisons of L value. Consequently, it is thought that the positive effect of honey solutions on the sheep heart tissue should be evaluated in organs with different parenchyma tissue and properties and whole cadaver.","PeriodicalId":23552,"journal":{"name":"Veterinaria","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78815637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-25DOI: 10.51607/22331360.2021.70.2.157
R. Milardović
The use of nuclear energy in veterinary medicine has been increasing over the last two decades. It mirrors the use in human health, but lags behind significantly. It is thought that animals are capable of undergoing almost any diagnostic or therapeutic procedure from human medicine. Strict rules about the use of nuclear energy in veterinary medicine are governed by the national authorities, and harmonized by the world leading authority in the field, the International Atomic Energy Agency (IAEA). Veterinary nuclear medicine has a clear methodology based on human medicine. Specificities relate to animals as patients, their lack of conscious cooperation, radiation safety measures in the light of animals’free movement, scarcety in trained staff and interest in referring at the side of veterinarians. Of primary concern remain the financial considerations, directly affecting the availability of veterinary nuclear services. Developed countries top the lists with services offered in veterinary nuclear medicine. Veterinary nuclear imaging can be preclinical for research purposes and clinical for diagnostic purposes. Isotopes of higher emission energies can be applied for therapeutic purposes to treat diseases. Such a combined use of isotopes for diagnostic and therapy gives a rise to the new field of theranostics. Nuclear images are created by injecting radioactive isotopes into animals, which then accumulate in the organs, depicting their morphology or function. Imaging is made by a scintillation gamma camera. Nuclear images are called nuclear scans, and the technique is called scintigraphy. Most commonly used radioactive isotope for diagnostic imaging is technetium Tc-99m, and for treatment iodine I-131. Scintigraphies most commonly performed on animals are thyroid, bone, liver and renal, all indicated in specific clinical scenarios. Number of both production and companion animals in the world is increasing. Consequently, demand for veterinary services has also been increasing, so that standard animal care needs constant improvement. That is why a need for more and novel diagnostic and therapeutic procedures, including the nuclear ones, will become more evident. Novel trends in veterinary nuclear medicine follow the trends in human medicine and are rapidly expanding. They include both research and clinical veterinary medicine.
{"title":"Veterinary Nuclear Medicine: A Look into the Future","authors":"R. Milardović","doi":"10.51607/22331360.2021.70.2.157","DOIUrl":"https://doi.org/10.51607/22331360.2021.70.2.157","url":null,"abstract":"The use of nuclear energy in veterinary medicine has been increasing over the last two decades. It mirrors the use in human health, but lags behind significantly. It is thought that animals are capable of undergoing almost any diagnostic or therapeutic procedure from human medicine. Strict rules about the use of nuclear energy in veterinary medicine are governed by the national authorities, and harmonized by the world leading authority in the field, the International Atomic Energy Agency (IAEA). Veterinary nuclear medicine has a clear methodology based on human medicine. Specificities relate to animals as patients, their lack of conscious cooperation, radiation safety measures in the light of animals’free movement, scarcety in trained staff and interest in referring at the side of veterinarians. Of primary concern remain the financial considerations, directly affecting the availability of veterinary nuclear services. Developed countries top the lists with services offered in veterinary nuclear medicine. Veterinary nuclear imaging can be preclinical for research purposes and clinical for diagnostic purposes. Isotopes of higher emission energies can be applied for therapeutic purposes to treat diseases. Such a combined use of isotopes for diagnostic and therapy gives a rise to the new field of theranostics. Nuclear images are created by injecting radioactive isotopes into animals, which then accumulate in the organs, depicting their morphology or function. Imaging is made by a scintillation gamma camera. Nuclear images are called nuclear scans, and the technique is called scintigraphy. Most commonly used radioactive isotope for diagnostic imaging is technetium Tc-99m, and for treatment iodine I-131. Scintigraphies most commonly performed on animals are thyroid, bone, liver and renal, all indicated in specific clinical scenarios. Number of both production and companion animals in the world is increasing. Consequently, demand for veterinary services has also been increasing, so that standard animal care needs constant improvement. That is why a need for more and novel diagnostic and therapeutic procedures, including the nuclear ones, will become more evident. Novel trends in veterinary nuclear medicine follow the trends in human medicine and are rapidly expanding. They include both research and clinical veterinary medicine.","PeriodicalId":23552,"journal":{"name":"Veterinaria","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83118633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-25DOI: 10.51607/22331360.2021.70.2.197
Widya Pintaka Bayu Putra, S. Aissanou, Nadir Boudjelal Dergal, A. Ayad
Principal component analysis (PCA) is important to describe the total phenotypic variance in livestock. Hence, the selection of livestock can be performed with the main component (PC1) of some phenotypic traits. This research was carried out to obtain the principal component (PC) in the morphostucture of male donkeys (Equus Asinus) at the Kabylie area of Algeria. Two different locations of Bejaia and Tizi-Ouzou province were chosen randomly. A total of 121 male donkeys (jackass) with an average age of 10.75±4.58 years were used in this study. Data of animals’ morphostructure consisted of 17 body measurements and 6 body indices. The PCA analysis in body measurements and body indexes were showed 5PC’s and 3PC’s, respectively that explain total variance in animals’ morphostructure. Besides, 5PC’s of body measurements and 3PC’s of body indexes had a total variance proportion of about 66.79% and 81.12%, respectively. According to Bartlett’s test, both PCA’s in this study were accurate (with significance of less than 0.05). According to Kaiser-Meyer-Olkin (KMO), the PCA of body indices was not accurate (KMO = 0.35). It was concluded that body measurements of chest depth, chest width, front-leg length and back length as the PC1 explain 32.16% of the total variance in animals’ morphostructure. In conclusion, the PCA of body measurements in this study is accurate with a high KMO value and significance of Bartlett’s test. The results of this study can be used as the basic information to develop a selection program for Algerian donkeys in the future.
{"title":"Principal component analysis (PCA) in the morphostructure of male Kabylie donkeys (Equus asinus), Algeria","authors":"Widya Pintaka Bayu Putra, S. Aissanou, Nadir Boudjelal Dergal, A. Ayad","doi":"10.51607/22331360.2021.70.2.197","DOIUrl":"https://doi.org/10.51607/22331360.2021.70.2.197","url":null,"abstract":"Principal component analysis (PCA) is important to describe the total phenotypic variance in livestock. Hence, the selection of livestock can be performed with the main component (PC1) of some phenotypic traits. This research was carried out to obtain the principal component (PC) in the morphostucture of male donkeys (Equus Asinus) at the Kabylie area of Algeria. Two different locations of Bejaia and Tizi-Ouzou province were chosen randomly. A total of 121 male donkeys (jackass) with an average age of 10.75±4.58 years were used in this study. Data of animals’ morphostructure consisted of 17 body measurements and 6 body indices. The PCA analysis in body measurements and body indexes were showed 5PC’s and 3PC’s, respectively that explain total variance in animals’ morphostructure. Besides, 5PC’s of body measurements and 3PC’s of body indexes had a total variance proportion of about 66.79% and 81.12%, respectively. According to Bartlett’s test, both PCA’s in this study were accurate (with significance of less than 0.05). According to Kaiser-Meyer-Olkin (KMO), the PCA of body indices was not accurate (KMO = 0.35). It was concluded that body measurements of chest depth, chest width, front-leg length and back length as the PC1 explain 32.16% of the total variance in animals’ morphostructure. In conclusion, the PCA of body measurements in this study is accurate with a high KMO value and significance of Bartlett’s test. The results of this study can be used as the basic information to develop a selection program for Algerian donkeys in the future.","PeriodicalId":23552,"journal":{"name":"Veterinaria","volume":"144 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74902829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tatiana Saporiti, Santiago Losiewics, A. Trelles, Cecilia Miraballes, F. Correa, U. Cuore
espanolEn Uruguay la problematica de la resistencia de Rhipicephalus microplus a los acaricidas habilitados ha ido incrementando con los anos, agravandose con la aparicion de poblaciones multirre-sistentes en 2009. El objetivo del trabajo fue analizar la resisten-cia de 47 poblaciones de garrapatas entre los anos 2017 y 2018 para cinco grupos quimicos: piretroides (cipermetrina, flumetri-na), organofosforados (ethion), amidinas (amitraz), fenilpirazo-les (fipronil) y lactonas macrociclicas (ivermectina), utilizando el Test de Paquete de Larvas (TLP) y una encuesta que se reali-zaba al responsable del establecimiento. De las 47 poblaciones analizadas 46 fueron resistentes a piretroides (46 cipermetrina, 45 flumetrina), presentando en su mayoria un alto nivel de resis-tencia. Todas las poblaciones muestreadas presentaron resisten-cia al menos a un grupo quimico. El 68,1 % a tres o mas grupos quimicos de forma simultanea pero ninguna presento resistencia ni susceptibilidad a los cinco analizados. Hubo poblaciones que presentaron resistencia a algunos grupos quimicos que no ha-bian sido utilizados en los establecimientos de los que fueron muestreadas. La mayoria de los predios realizaron un numero mayor de tratamientos al ano que el recomendado para un trata-miento generacional. Para todos los principios activos evaluados hubo poblaciones con sospechas de resistencia que no se confir-maron mediante TPL. Esto refleja las situaciones complejas que presentan hoy estos establecimientos en torno a la resistencia a los acaricidas por parte de R. microplus, que podria estar vin-culada al modo, frecuencia y tiempo de uso de estos acaricidas. Asi como tambien a su uso pero con otros fines y a los mo-vimientos, ingresos principalmente, de animales a los predios EnglishAcaricide resistance of Rhipicephalus microplus tick in Uruguay means a serious problem that has been worsening through years hitting a high point with the detection of multiresistant popula-tions on 2009. Therefore, the aim of this study was to analyze resistance for five chemical groups: pyrethroids (cypermethrin, flumethrin), organophosphates (ethion), amidines (amitraz), phenylpyrazoles (fipronil), macrocyclic lactones (ivermectin) on 47 populations between years 2017 and 2018 by using the Larval Packet Test (LPT) and a quiz made to the responsible for the farm. Results show that 46 from the 47 populations analyzed were resistant to pyrethroids (46 to cypermethrina, 45 to flume-thryn). This chemical group also showed the largest number of resistant populations with a high grade of resistance. Every pop-ulation showed to be resistant to at least one chemical group fur-thermore the 68.1 % of the populations were resistant to at least 3 chemical groups simultaneously but no population was diag-nosed resistant to the 5 chemical groups analyzed neither to be susceptible to all 5 chemical groups. It stands out that there were populations resistant to some chemical groups that the farms of origin ha
在乌拉圭，微头滴虫对杀螨剂的耐药性问题多年来一直在增加，2009年出现了多种群。工作的目标是分析蜱虫种群resisten-cia 47年2017年和2018年五个团体之间化学物质:除虫菊酯(氰戊菊酯、flumetri-na (ethion有机磷)、amidinas(甲)fenilpirazo-les(氟虫腈和lactonas macrociclicas(伊维菌素),使用测试包幼虫(月内)和一项民调reali-zaba负责建立。在本研究中，我们分析了对拟除虫菊酯(46种氯氰菊酯，45种氟氰菊酯)的抗性，其中大多数具有较高的抗性水平。在本研究中，我们分析了两种不同的化学基团，一种是抗氧化基团，另一种是抗氧化基团。68.1%同时对三种或三种以上化学组，但对所有五种化学组均无耐药性或易感性。对某些化学群表现出耐药性的人群在抽样的设施中没有使用过。大多数农场每年进行的处理次数超过了推荐的世代处理次数。对于所有被评估的活性成分，都有未被TPL证实的怀疑耐药人群。这反映了这些机构目前关于微加鼠对杀螨剂的抗性的复杂情况，这可能与使用这些杀螨剂的方式、频率和时间有关。就是太但其他目的和用途mo-vimientos,主要的收入、动物训练场EnglishAcaricide resistance of Rhipicephalus microplus滴答in乌拉圭means严重problem that has been worsening through》" (a high point with the检测of multiresistant popula-tions on 2009年。因此,the aim of this study was to analyze resistance for five化学:pyrethroids (cypermethrin群组,flumethrin organophosphates (ethion)、amidines(甲)phenylpyrazoles(氟虫腈)、macrocyclic lactones (ivermectin) on 47人口between 2017年和2018年by using the Test(官阶幼虫Packet) and a quiz made to the responsible for the农场。结果显示，47个分析群体中有46个对除虫菊酯有耐药性(46个对氯菊酯有耐药性，45个对氟菊酯有耐药性)。该化学组还显示了数量最多的耐药种群，具有很高的耐药程度。每个pop-ulation显示对至少一个化学组具有抗性，但68.1%的人口同时对至少3个化学组具有抗性，但没有一个人口被诊断对所有5个化学组具有抗性。它表明，有一些种群对某些化学群具有抗性，而这些化学群的起源农场从未使用过。据记录，每年为控制R. microplus而进行的治疗次数比建议的代际治疗次数要多。对于所评估的每一种活性成分，都有耐药性的怀疑，幼虫包试验无法证实。这反映了这些农场在微鼠杀螨抗性方面的复杂情况。这可能与这些农场使用杀螨剂的方式、频率和时间有关，也可能与将杀螨剂用于其他目的有关，也可能与从其他来源感染小鼠的动物进入的影响有关。
{"title":"Análisis del perfil de susceptibilidad de la garrapata Rhipicephalus microplus para cinco grupos químicos y factores asociados en poblaciones de campo del norte de Uruguay","authors":"Tatiana Saporiti, Santiago Losiewics, A. Trelles, Cecilia Miraballes, F. Correa, U. Cuore","doi":"10.29155/VET.57.215.5","DOIUrl":"https://doi.org/10.29155/VET.57.215.5","url":null,"abstract":"espanolEn Uruguay la problematica de la resistencia de Rhipicephalus microplus a los acaricidas habilitados ha ido incrementando con los anos, agravandose con la aparicion de poblaciones multirre-sistentes en 2009. El objetivo del trabajo fue analizar la resisten-cia de 47 poblaciones de garrapatas entre los anos 2017 y 2018 para cinco grupos quimicos: piretroides (cipermetrina, flumetri-na), organofosforados (ethion), amidinas (amitraz), fenilpirazo-les (fipronil) y lactonas macrociclicas (ivermectina), utilizando el Test de Paquete de Larvas (TLP) y una encuesta que se reali-zaba al responsable del establecimiento. De las 47 poblaciones analizadas 46 fueron resistentes a piretroides (46 cipermetrina, 45 flumetrina), presentando en su mayoria un alto nivel de resis-tencia. Todas las poblaciones muestreadas presentaron resisten-cia al menos a un grupo quimico. El 68,1 % a tres o mas grupos quimicos de forma simultanea pero ninguna presento resistencia ni susceptibilidad a los cinco analizados. Hubo poblaciones que presentaron resistencia a algunos grupos quimicos que no ha-bian sido utilizados en los establecimientos de los que fueron muestreadas. La mayoria de los predios realizaron un numero mayor de tratamientos al ano que el recomendado para un trata-miento generacional. Para todos los principios activos evaluados hubo poblaciones con sospechas de resistencia que no se confir-maron mediante TPL. Esto refleja las situaciones complejas que presentan hoy estos establecimientos en torno a la resistencia a los acaricidas por parte de R. microplus, que podria estar vin-culada al modo, frecuencia y tiempo de uso de estos acaricidas. Asi como tambien a su uso pero con otros fines y a los mo-vimientos, ingresos principalmente, de animales a los predios EnglishAcaricide resistance of Rhipicephalus microplus tick in Uruguay means a serious problem that has been worsening through years hitting a high point with the detection of multiresistant popula-tions on 2009. Therefore, the aim of this study was to analyze resistance for five chemical groups: pyrethroids (cypermethrin, flumethrin), organophosphates (ethion), amidines (amitraz), phenylpyrazoles (fipronil), macrocyclic lactones (ivermectin) on 47 populations between years 2017 and 2018 by using the Larval Packet Test (LPT) and a quiz made to the responsible for the farm. Results show that 46 from the 47 populations analyzed were resistant to pyrethroids (46 to cypermethrina, 45 to flume-thryn). This chemical group also showed the largest number of resistant populations with a high grade of resistance. Every pop-ulation showed to be resistant to at least one chemical group fur-thermore the 68.1 % of the populations were resistant to at least 3 chemical groups simultaneously but no population was diag-nosed resistant to the 5 chemical groups analyzed neither to be susceptible to all 5 chemical groups. It stands out that there were populations resistant to some chemical groups that the farms of origin ha","PeriodicalId":23552,"journal":{"name":"Veterinaria","volume":"2 1","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85690095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Producción científica del área agrarias de Uruguay","authors":"R. Ungerfeld","doi":"10.29155/VET57.215.7","DOIUrl":"https://doi.org/10.29155/VET57.215.7","url":null,"abstract":"","PeriodicalId":23552,"journal":{"name":"Veterinaria","volume":"21 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74342964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Valentin Bazzano, Jorge Freire, M. T. Armua-Fernandez, M. Félix, Luis A. Carvalho, J. Venzal
Hepatozoonosis is a parasitic disease caused by Hepatozoon spp. In general, it has a subclinical progression, and its symptoms are associated with an induced immunodeficiency triggered by a concomitant disease. The aim of this study was to detect by PCR the presence of Hepatozoon spp. in domestic cats from Uruguay. A total of 230 blood samples were analyzed obtained in nine of the 19 departments of Uruguay. Only were included samples from cats with early diagnosis of clinical anemia. For molecular diagnosis, two PCRs targeting two overlapping regions of 18S rRNA gene for Hepatozoon spp. were carried out. Nine out of 230 samples were positive for Hepatozoon spp., resulting in an infection frequency of 3.91%. The obtained sequences revealed 99.10 to 100% identity with Hepatozoon felis sequences registered in GenBank. Moreover, the phylogenetic analysis showed that the sequences obtained in this study clustered, with a high Bootstrap support with sequences of H. felis from different continents. To our knowledge, this is the first report of H. felis infecting domestic cats in Uruguay.
{"title":"Detección molecular de Hepatozoon felis en gatos con anemia en Uruguay","authors":"Valentin Bazzano, Jorge Freire, M. T. Armua-Fernandez, M. Félix, Luis A. Carvalho, J. Venzal","doi":"10.29155/VET.57.215.4","DOIUrl":"https://doi.org/10.29155/VET.57.215.4","url":null,"abstract":"Hepatozoonosis is a parasitic disease caused by Hepatozoon spp. In general, it has a subclinical progression, and its symptoms are associated with an induced immunodeficiency triggered by a concomitant disease. The aim of this study was to detect by PCR the presence of Hepatozoon spp. in domestic cats from Uruguay. A total of 230 blood samples were analyzed obtained in nine of the 19 departments of Uruguay. Only were included samples from cats with early diagnosis of clinical anemia. For molecular diagnosis, two PCRs targeting two overlapping regions of 18S rRNA gene for Hepatozoon spp. were carried out. Nine out of 230 samples were positive for Hepatozoon spp., resulting in an infection frequency of 3.91%. The obtained sequences revealed 99.10 to 100% identity with Hepatozoon felis sequences registered in GenBank. Moreover, the phylogenetic analysis showed that the sequences obtained in this study clustered, with a high Bootstrap support with sequences of H. felis from different continents. To our knowledge, this is the first report of H. felis infecting domestic cats in Uruguay.","PeriodicalId":23552,"journal":{"name":"Veterinaria","volume":"6 1","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2021-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75791569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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