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WSC volume 71 issue 1 Cover and Front matter 《华尔街日报》第71卷第1期封面和封面
IF 2.5 2区 农林科学 Q2 AGRONOMY Pub Date : 2023-01-01 DOI: 10.1017/wsc.2023.13
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引用次数: 0
Determining How Almond (Prunus dulcis) Harvest and Processing Contributes to Low Levels of Glyphosate and Glufosinate Residues in Almonds 确定杏仁(Prunus dulcis)的收获和加工对杏仁中草甘膦和草铵膦残留量的影响
IF 2.5 2区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-23 DOI: 10.1017/wsc.2022.67
Katie Martin, B. Hanson
Abstract California is the top producer of almonds [Prunus dulcis (Mill.) D.A. Webb] worldwide, generating more than $6 billion in revenue in 2020; the European Union (EU) is the primary importer of California almonds. Weed control in almond orchards is an important part of the preharvest process, because weeds can interfere with harvest equipment and host diseases. Glyphosate and glufosinate are broad-spectrum herbicides commonly used for preharvest weed control. Global differences in maximum residue limits (MRLs) and regulated compounds can pose a challenge for growers who rely on broad-spectrum herbicides such as glyphosate and glufosinate for preharvest weed control. The EU MRL for glyphosate and total glufosinate is currently 0.1 mg kg–1. The U.S. MRL for total glyphosate is 1 mg kg–1, and total glufosinate is 0.5 mg kg–1. An 8-wk field experiment, from spray to harvest, was conducted in an 8-ha commercial orchard to evaluate the potential contribution of the preharvest herbicide treatment to low levels of herbicide residue in almonds. Then, the same batch of almonds was followed through a commercial processing facility to evaluate the potential movement of herbicide residues from soil, debris, and hulls to almond kernels during processing. Glyphosate was not detected in any almond kernel samples at the end of processing. A glufosinate metabolite, 3-(methylphosphinico)propionic acid (MPP), was detected in kernels at the end of processing at about 0.1 mg kg–1, which is above the EU MRL for total glufosinate. Almonds sampled directly from the tree, without any contact with soil, were found to have elevated MPP residues. This indicates glufosinate or MPP translocation may be a factor in low-level glufosinate residues detected in almonds in some EU exports.
摘要加利福尼亚州是全球最大的杏仁生产国,2020年的收入超过60亿美元;欧盟是加州杏仁的主要进口国。杏仁园的杂草控制是采前过程的重要组成部分,因为杂草会干扰收获设备和寄主疾病。草甘膦和草膦是常用于采前杂草控制的广谱除草剂。最大残留限量(MRL)和受管制化合物的全球差异可能会给依赖草甘膦和草膦等广谱除草剂进行采前杂草控制的种植者带来挑战。目前,欧盟草甘膦和草膦总含量的最大残留限量为0.1 mg kg-1。美国草甘膦总量的最大残留限量为1 mg kg–1,草膦总量为0.5 mg kg–1。在一个8公顷的商业果园中进行了从喷洒到收获的8周田间试验,以评估采前除草剂处理对杏仁中低水平除草剂残留的潜在贡献。然后,对同一批杏仁进行商业加工,以评估除草剂残留物在加工过程中从土壤、碎片和外壳向杏仁粒的潜在移动。加工结束时,未在任何杏仁仁样品中检测到草甘膦。在加工结束时,在谷粒中检测到约0.1 mg kg–1的草膦代谢产物3-(甲基膦)丙酸(MPP),高于欧盟总草膦的MRL。在不与土壤接触的情况下,直接从树上取样的杏仁被发现具有较高的MPP残留量。这表明,在一些欧盟出口的杏仁中检测到的低水平草酸盐残留可能是一个因素。
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引用次数: 0
Utilization of Image-Based Spectral Reflectance to Detect Herbicide Resistance in Glufosinate-Resistant and Glufosinate-Susceptible Plants: A Proof of Concept 利用基于图像的光谱反射率检测草铵膦抗性和草铵膦敏感植物的除草剂抗性:概念验证
IF 2.5 2区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-19 DOI: 10.1017/wsc.2022.68
E. Jones, R. Austin, J. Dunne, C. Cahoon, Katherine M. Jennings, R. León, W. Everman
Abstract Glufosinate is an effective postemergence herbicide, and overreliance on this herbicide for weed control is likely to increase and select for glufosinate-resistant weeds. Common assays to confirm herbicide resistance are dose–response and molecular sequencing techniques; both can require significant time, labor, unique technical equipment, and a specialized skillset to perform. As an alternative, we propose an image-based approach that uses a relatively inexpensive multispectral sensor designed for unmanned aerial vehicles to measure and quantify surface reflectance from glufosinate-treated leaf disks. Leaf disks were excised from a glufosinate-resistant and glufosinate-susceptible corn (Zea mays L.), cotton (Gossypium hirsutum L.), and soybean [Glycine max (L.) Merr.] varieties and placed into a 24-well plate containing eight different concentrations (0 to 10 mM) of glufosinate for 48 h. Multispectral images were collected after the 48-h incubation period across five discrete wave bands: blue (475 to 507 nm), green (560 to 587 nm), red (668to 682 nm), red edge (717 to 729 nm), and near infrared (842 to 899 nm). The green leaf index (GLI; a metric to measure chlorophyll content) was utilized to determine relationships between measured reflectance from the tested wave bands from the treated leaf disks and the glufosinate concentration. Clear differences of spectral reflectance were observed between the corn, cotton, and soybean leaf disks of the glufosinate-resistant and glufosinate-susceptible varieties at the 10 mM concentration for select wave bands and GLI. Leaf disks from two additional glufosinate-resistant and glufosinate-susceptible varieties of each crop were subjected to a similar assay with two concentrations: 0 and 10 mM. No differences of spectral reflectance were observed from the corn and soybean varieties in all wave bands and the GLI. The leaf disks of the glufosinate-resistant and glufosinate-susceptible cotton varieties were spectrally distinct in the green, blue, and red-edge wave bands. The results provide a basis for rapidly detecting glufosinate-resistant plants via spectral reflectance. Future research will need to determine the glufosinate concentrations, useful wave bands, and susceptible/resistant thresholds for weeds that evolve resistance.
摘要:草铵膦是一种有效的羽化后除草剂,过度依赖草铵膦来控制杂草可能会增加对草铵膦抗性杂草的选择。确定除草剂抗性的常用检测方法是剂量反应和分子测序技术;两者都需要大量的时间、劳动力、独特的技术设备和专门的技能来完成。作为替代方案,我们提出了一种基于图像的方法,该方法使用一种相对便宜的多光谱传感器,设计用于无人机,以测量和量化草铵膦处理过的叶片的表面反射率。从抗草铵膦和对草铵膦敏感的玉米(Zea mays L.)、棉花(Gossypium hirsutum L.)和大豆(Glycine max (L.))上切除叶片。稳定。放置在含有8种不同浓度(0至10 mM)草铵膦的24孔板中48小时。孵育48小时后,在5个不同波段收集多光谱图像:蓝色(475至507 nm)、绿色(560至587 nm)、红色(668至682 nm)、红边(717至729 nm)和近红外(842至899 nm)。绿叶指数(GLI;利用测量叶绿素含量的度量来确定从处理过的叶片的测试波段测量的反射率与草铵膦浓度之间的关系。在10 mM浓度下,玉米、棉花和大豆抗草铵膦品种叶片的光谱反射率和GLI存在明显差异。另外两种作物的抗草铵膦和感草铵膦品种的叶盘进行了类似的试验,浓度分别为0和10 mM。在所有波段和GLI中,玉米和大豆品种的光谱反射率没有差异。抗草铵膦和感草铵膦棉花品种的叶片在绿、蓝、红边波段具有明显的光谱差异。研究结果为利用光谱反射率快速检测草铵膦抗性植物提供了依据。未来的研究将需要确定草铵膦浓度,有用的波段,以及对杂草产生抗性的敏感/抗性阈值。
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引用次数: 2
Identification and Extraction of Herbicidal Compounds from Metabolites of Trichoderma Polysporum HZ-31 多孢木霉HZ-31代谢产物中除草化合物的鉴定与提取
IF 2.5 2区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-15 DOI: 10.1017/wsc.2022.66
Haixia Zhu, Hongyu Chen, Yongqiang Ma, Q. Guo
Abstract Trichoderma polysporum (Link) Rifai HZ-31 fermentation broth was separated and purified by extraction, column chromatography, and high-performance liquid chromatography. Four monomer compounds with strong herbicidal activity were obtained: p-hydroxyphenyl-2,3-dihydroxypropyl ether, o-hydroxy-3-carbonyl-1-phenylpropanol, 1,8-propanediol o-xylene, and 2-3-dihydroxypropyl propionate. The biological activity verification test indicated that the four monomer compounds could inhibit the germination of wild oat (Avena fatua L.) and canola (Brassica napus L.) seeds. Of the four, compound 3 (1,8-propanediol o-xylene) had obvious inhibitory effects on the germination of A. fatua and B. napus seeds, with inhibition rates of 83.33% and 86.67%, respectively. Therefore, the identification of this monomer compound lays a foundation for the further development of a novel microbial herbicide by directly utilizing it and developing new derivatives with herbicidal functions as lead compounds.
摘要采用提取、柱色谱、高效液相色谱等方法对日发HZ-31木霉发酵液进行了分离纯化。得到了4个具有较强除草活性的单体化合物:对羟基苯基-2,3-二羟丙基醚、邻羟基-3-羰基-1-苯基丙醇、1,8-丙二醇邻二甲苯和2-3-二羟基丙酸酯。生物活性验证试验表明,四种单体化合物均能抑制野生燕麦(Avena fatua L.)和油菜(Brassica napus L.)种子的发芽。其中,化合物3(1,8-丙二醇-邻二甲苯)对甘蓝型油菜和甘蓝型油菜种子的发芽有明显的抑制作用,抑制率分别为83.33%和86.67%。因此,该单体化合物的鉴定为直接利用它并开发具有除草功能的新衍生物作为先导化合物,进一步开发新型微生物除草剂奠定了基础。
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引用次数: 0
Early and On-Ground Image-Based Detection of Poppy (Papaver rhoeas) in Wheat Using YOLO Architectures 基于YOLO结构的小麦罂粟早期和地面图像检测
IF 2.5 2区 农林科学 Q2 AGRONOMY Pub Date : 2022-12-15 DOI: 10.1017/wsc.2022.64
Fernando J. Pérez-Porras, J. Torres-Sánchez, F. López-Granados, F. Mesas-Carrascosa
Abstract Poppy (also common poppy or corn poppy; Papaver rhoeas L., PAPRH) is one of the most harmful weeds in winter cereals. Knowing the precise and accurate location of weeds is essential for developing effective site-specific weed management (SSWM) for optimized herbicide use. Among the available tools for weed mapping, deep learning (DL) is used for its accuracy and ability to work in complex scenarios. Crops represent intricate situations for weed detection, as crop residues, occlusion of weeds, or spectral similarities between crop and weed seedlings are frequent. Timely discrimination of weeds is needed, because postemergence herbicides are used just when weeds and crops are at an early growth stage. This study addressed P. rhoeas early detection in wheat (Triticum spp.) by comparing the performance of six DL-based object-detection models focused on the “You Only Look Once” (YOLO) architecture (v3 to v5) using proximal RGB images to train the models. The models were assessed using open-source software, and evaluation offered a range of results for quality of recognition of P. rhoeas as well as computational capacity during the inference process. Of all the models, YOLOv5s performed best in the testing phase (75.3%, 76.2%, and 77% for F1-score, mean average precision, and accuracy, respectively). These results indicated that under real field conditions, DL-based object-detection strategies can identify P. rhoeas at an early stage, providing accurate information for developing SSWM.
摘要罂粟(也称为普通罂粟或玉米罂粟;Papaver rhoeas L.,PAPRH)是冬季谷物中危害最大的杂草之一。了解杂草的精确位置对于开发有效的特定地点杂草管理(SSWM)以优化除草剂的使用至关重要。在可用的杂草映射工具中,深度学习(DL)因其准确性和在复杂场景中工作的能力而被使用。作物代表了杂草检测的复杂情况,因为作物残留物、杂草堵塞或作物和杂草幼苗之间的光谱相似性很常见。需要及时识别杂草,因为在杂草和作物处于早期生长阶段时才使用出苗后除草剂。这项研究通过比较六个基于DL的对象检测模型的性能来解决小麦(Triticum spp.)中的P.rheas早期检测问题,这些模型侧重于“你只看一次”(YOLO)架构(v3到v5),使用近端RGB图像来训练模型。使用开源软件对模型进行了评估,评估提供了一系列关于P.rheas识别质量以及推理过程中计算能力的结果。在所有模型中,YOLOv5在测试阶段表现最好(F1得分、平均精度和准确度分别为75.3%、76.2%和77%)。这些结果表明,在真实场条件下,基于DL的目标检测策略可以在早期识别P.rheas,为开发SSWM提供准确的信息。
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引用次数: 2
Target-Site and Metabolic Mechanisms of Tolerance to Penoxsulam in Pond Lovegrass (Eragrostis japonica) 池塘矮种草(Eragrostis japonica)对百灵草(Penoxsulam)耐受性的靶位点及其代谢机制
IF 2.5 2区 农林科学 Q2 AGRONOMY Pub Date : 2022-11-17 DOI: 10.1017/wsc.2022.63
Ying Liu, Hao Wang, Jiapeng Fang, Haitao Gao, Jinyi Chen, Zhen Peng, Liyao Dong
Abstract The identification of herbicide tolerance is essential for effective chemical weed control. According to whole-plant dose–response assays, none of 29 pond lovegrass [Eragrostis japonica (Thunb.) Trin.] populations were sensitive to penoxsulam. The effective dose values of penoxsulam causing 50% inhibition of fresh weight (GR50: 105.14 to 148.78 g ai ha–1) in E. japonica populations were much higher than the label rate of penoxsulam (15 to 30 g ai ha–1) in the field. This confirmed that E. japonica was tolerant to penoxsulam. Eragrostis japonica populations showed 52.83- to 74.76-fold higher tolerance to penoxsulam than susceptible barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.]. The mechanisms of tolerance to penoxsulam in E. japonica were also identified. In vitro activity assays revealed that the penoxsulam concentration required to inhibit 50% of the acetolactate synthase (ALS) activity (IC50) was 12.27-fold higher in E. japonica than in E. crus-galli. However, differences in the ALS gene, previously found to endow target-site resistance in weeds, were not detected in the sequences obtained. Additionally, the expression level of genes encoding ALS in E. japonica was approximately 2-fold higher than in E. crus-galli after penoxsulam treatment. Furthermore, penoxsulam tolerance can be significantly reversed by three cytochrome P450 monooxygenase (CytP450) inhibitors (1-aminobenzotriazole, piperonyl butoxide, and malathion), and the activity of NADPH-dependent cytochrome P450 reductase toward penoxsulam in E. japonica increased significantly (approximately 7-fold higher) compared with that of treated E. crus-galli. Taken together, these results indicate that lower ALS sensitivity, relatively higher ALS expression levels, and stronger metabolism of CytP450s combined to bring about penoxsulam tolerance in E. japonica.
摘要除草剂耐受性的鉴定对于有效的化学除草至关重要。根据全株剂量-反应测定,29个池塘爱情草[Eagrostis japonica(Thunb.)Trin.]种群中没有一个对penoxsulam敏感。在E.japonica种群中,本草对鲜重抑制50%的有效剂量值(GR50:105.14至148.78 g ai ha–1)远高于本草在田间的标记率(15至30 g ai ha-1)。这证实了E.japonica对penoxsulam具有耐受性。日本Eragrostis群体对penoxsulam的耐受性是感病Barnyardras[Echinochloa crus galli(L.)P.Beauv.]的52.83至74.76倍。体外活性测定显示,抑制50%乙酰乳酸合成酶(ALS)活性(IC50)所需的penoxsulam浓度在日本E.japonica中比在鸡E.crus中高12.27倍。然而,先前发现在杂草中赋予靶位点抗性的ALS基因的差异在所获得的序列中没有检测到。此外,在penoxsulam处理后,编码ALS的基因在E.japonica中的表达水平大约是E.crus galli的2倍。此外,三种细胞色素P450单加氧酶(CytP450)抑制剂(1-氨基苯并三唑、哌啶丁氧基和马拉硫磷)可以显著逆转对penoxsulam的耐受性,并且与处理过的鸡E.crus galli相比,日本E.japonica中NADPH依赖性细胞色素P450reductase对penoxsulam的活性显著增加(约高出7倍)。总之,这些结果表明,较低的ALS敏感性、相对较高的ALS表达水平和更强的CytP450s代谢相结合,导致了E.japonica对penoxsulam的耐受性。
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引用次数: 0
Editorial for Weed science, Volume 71 杂草科学编辑,第71卷
IF 2.5 2区 农林科学 Q2 AGRONOMY Pub Date : 2022-11-17 DOI: 10.1017/wsc.2022.57
W. Vencill
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引用次数: 0
Herbicides in Unexpected Places: Non-Target Impacts from Tree Root Exudation of Aminopyralid and Triclopyr Following Basal Bark Treatments of Invasive Chokecherry (Prunus padus) in Alaska 意想不到的地方的除草剂:阿拉斯加入侵樱桃(Prunus padus)基树皮处理后,树根渗出的氨基吡啶和三氯吡啶的非目标影响
IF 2.5 2区 农林科学 Q2 AGRONOMY Pub Date : 2022-11-01 DOI: 10.1017/wsc.2022.61
Gino Graziano, P. Tomco, S. Seefeldt, C. Mulder, Zachary C. Redman
Abstract Basal bark treatment of invasive trees is an approach designed to limit damage to non-target vegetation in the vicinity, but non-target injury is still documented. No study of basal bark treatments has examined the release of herbicide residues from roots of treated plants and resulting non-target impacts. Studies were conducted in Alaska interior and coastal boreal forests on basal bark treatments with aminopyralid and triclopyr on active-growth and dormant invasive chokecherry (Prunus padus L.). The study assessed non-target damage and soil herbicide residue using a combination of visual evaluations, bioassays, and soil residue analyses. Non-target damage from herbicide residues were identified in 40% of treatments containing aminopyralid with triclopyr, 60% of treatments containing aminopyralid alone, and 5% of treatments containing only triclopyr. Laboratory studies of aminopyralid treatments to saplings isolated the effects of herbicide exudation from roots, which was found to be significant, and the magnitude was dependent on dose. Herbicide soil residues in field and laboratory experiments were quantified with analytical detection and plant bioassays. Aminopyralid soil residues were identified in 57% of field treatments receiving 8 to 60 ml of herbicide solution (2% ai) and 70% of laboratory treatments receiving 10 µl of herbicide solution (2% to 16% ai). Triclopyr residues were found from one field treatment following dosage with 28 ml of herbicide solution (18.5% ai). Anatomically, plants grown in soils associated with herbicide-treated trees, both in the field and lab, grew less dry mass than non–herbicide treated controls. This study provides the first evidence that root exudation of herbicide following basal bark treatments contributes to non-target damage of adjacent vegetation and to accumulation of soil herbicide residues. This is an important new factor for integrated pest management within basal bark treatment systems and has implications for other herbicide application types such as injections and frill, as well as determining whether root exudation is species or herbicide specific.
摘要入侵树木的基底树皮处理是一种旨在限制对附近非目标植被损害的方法,但非目标损伤仍有文献记载。没有对基础树皮处理的研究检测过除草剂残留物从处理过的植物根部的释放以及由此产生的非目标影响。在阿拉斯加内陆和沿海北方森林进行了研究,研究了氨基吡喃和三氯吡对活跃生长和休眠入侵樱桃(Prunus padus L.)的基础树皮处理。该研究结合视觉评估、生物测定和土壤残留分析,评估了非目标损害和土壤除草剂残留。在40%含有氨基吡喃和三氯吡的处理中,60%单独含有氨基吡吡喃的处理和5%仅含有三氯吡处理中,发现了除草剂残留造成的非目标损害。对吡喃氨基脲处理幼树的实验室研究分离出除草剂从根部渗出的影响,发现这种影响是显著的,其程度取决于剂量。通过分析检测和植物生物测定对田间和实验室实验中除草剂土壤残留量进行了定量。在接受8至60毫升除草剂溶液(2%ai)的57%的田间处理和接受10µl除草剂溶液(2至16%ai)的70%的实验室处理中,发现了吡喃氨基脲的土壤残留。在用28毫升除草剂溶液(18.5%ai)给药后的一次田间处理中发现了三氯吡残留。从解剖学上讲,生长在与除草剂处理过的树木相关的土壤中的植物,无论是在野外还是实验室,其干重都低于未经除草剂处理的对照。这项研究首次证明,基础树皮处理后除草剂的根系渗出会对邻近植被造成非目标损害,并导致土壤除草剂残留的积累。这是在基础树皮处理系统内进行害虫综合管理的一个重要的新因素,对其他除草剂应用类型(如注射和修剪)以及确定根系分泌物是物种还是除草剂特异性都有影响。
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引用次数: 0
Crop Physiological Considerations for Combining Variable-Density Planting to Optimize Seed Costs and Weed Suppression 结合变密度种植优化种子成本和杂草抑制的作物生理考虑
IF 2.5 2区 农林科学 Q2 AGRONOMY Pub Date : 2022-11-01 DOI: 10.1017/wsc.2022.62
Sandra R. Ethridge, Anna M. Locke, W. Everman, D. Jordan, R. León
Abstract High crop densities are valuable to increase weed suppression, but growers might be reluctant to implement this practice due to increased seed cost. Because it is also possible to lower planting densities in areas with no or low weed interference risk, the area allocated to each planting density must be optimized considering seed cost and productivity per plant. In this study, the growth and yield of maize (Zea mays L.), cotton (Gossypium hirsutum L.), and soybean [Glycine max (L.) Merr.] were characterized in response to low planting densities and arrangements. The results were used to develop a bioeconomic model to optimize the area devoted to high- and low-density plantings to increase weed suppression without increasing seed cost. Physiological differences seen in each crop varied with the densities tested; however, maize was the only crop that had differences in yield (per area) between densities. When a model to optimize low and high planting densities was used, maize and cotton showed the most plasticity in yield per planted seed (g seed–1) and area of low density to compensate for high-density area unit. Maize grown at 75% planting density compared with the high-planting density (200%) increased yield (g seed–1) by 229%, return by 43%, and profit by 79% while decreasing the low-density area needed to compensate for high-density area. Cotton planted at 25% planting density compared with the 200% planting density increased yield (g seed–1) by 1,099%, return by 46%, and profit by 62% while decreasing the low-density area needed to compensate for high-density area. In contrast, the high morphological plasticity of soybean did not translate into changes in area optimization, as soybean maintained return, profit, and a 1:1 ratio for area compensation. This optimization model could allow for the use of variable planting at large scales to increase weed suppression while minimizing costs to producers.
摘要高作物密度对增加杂草抑制有价值,但由于种子成本增加,种植者可能不愿意实施这种做法。由于在没有杂草干扰风险或杂草干扰风险较低的地区也可以降低种植密度,因此必须考虑每株植物的种子成本和生产力来优化分配给每种种植密度的面积。在本研究中,玉米(Zea mays L.)、棉花(Gossypium hirsutum L.)和大豆(Glycine max(L.)Merr.])的生长和产量对低种植密度和安排的响应进行了表征。研究结果被用于开发一个生物经济模型,以优化高密度和低密度种植的面积,从而在不增加种子成本的情况下提高杂草抑制率。每种作物的生理差异随着测试密度的不同而不同;然而,玉米是唯一一种密度之间产量(单位面积)存在差异的作物。当使用优化低密度和高密度种植的模型时,玉米和棉花在每种种子的产量(g种子-1)和低密度面积方面表现出最大的可塑性,以补偿高密度面积单位。与高种植密度(200%)相比,种植密度为75%的玉米产量(g种子-1)增加了229%,收益增加了43%,利润增加了79%,同时减少了需要补偿高密度区域的低密度区域。与200%的种植密度相比,25%的种植密度种植的棉花产量(g种子-1)增加了1099%,回报率增加了46%,利润增加了62%,同时减少了需要补偿高密度区域的低密度区域。相比之下,大豆的高形态可塑性并没有转化为面积优化的变化,因为大豆保持了回报、利润和1:1的面积补偿比例。这种优化模型可以允许大规模使用可变种植,以增加杂草抑制,同时最大限度地降低生产者的成本。
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引用次数: 0
WSC volume 70 issue 6 Cover and Front matter 《华尔街日报》第70卷第6期封面和封面
IF 2.5 2区 农林科学 Q2 AGRONOMY Pub Date : 2022-11-01 DOI: 10.1017/wsc.2022.65
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引用次数: 0
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