Pub Date : 2023-09-28DOI: 10.14739/2310-1210.2023.5.282107
V. V. Syvolap, A. O. Bohun
Aim. To study the features of the left ventricle remodeling and changes in its functional signs in patients with atrial fibrillation (AF). Materials and methods. In total, 2423 patients aged from 18 to 94 years (mean age – 57.9 ± 16.4 years), 51 % men, with pathologies of the cardiovascular and respiratory systems and patients without diagnosed diseases of cardiovascular system were enrolled in the study. Echocardiography was performed on an Esaote MyLab Seven device (Italy) according to generally accepted rules. The indicators of systolic and diastolic, valvular functions, the distribution of patients according to four classic types of the left ventricular geometry were studied. Statistical analysis was performed using the Statistica 13.0 software package for Windows. Statistically significant differences were calculated using the Mann–Whitney U test, Pearson’s χ2 test, Kruskal–Wallis test. A level of p < 0.05 was taken to indicate statistical significance. Results. The prevalence of AF in the group of normal geometry was 6.5 %, concentric remodeling – 11.8 %, eccentric hypertrophy – 17.4 %, concentric hypertrophy – 21.7 %. Left ventricular hypertrophy was diagnosed in 56.2 % of patients with AF (32.0 % – eccentric hypertrophy, 27.8 % – concentric hypertrophy), while in the patient group without AF, left ventricular hypertrophy was detected in only 33.9 % of the examined (20.4 % – eccentric hypertrophy, 13.5 % – concentric hypertrophy). In groups of concentric and eccentric hypertrophy, the patients were older, there was a higher prevalence of mitral, aortic, tricuspid valve regurgitation, and lower indicators of systolic function (EF, TEI, S’), diastolic function (e’med) than those in groups with normal geometry and concentric remodeling. In 29.3 % of patients with AF, the geometry of the left ventricle remained normal. Conclusions. The prevalence of AF increased according to the geometric patterns of the left ventricular remodeling with the highest rates in the groups of eccentric and concentric hypertrophy, which were also associated with worse indicators of systolic, diastolic, and valve functions.
{"title":"Types of the left ventricle geometry and changes in functional parameters of the heart in patients with atrial fibrillation","authors":"V. V. Syvolap, A. O. Bohun","doi":"10.14739/2310-1210.2023.5.282107","DOIUrl":"https://doi.org/10.14739/2310-1210.2023.5.282107","url":null,"abstract":"Aim. To study the features of the left ventricle remodeling and changes in its functional signs in patients with atrial fibrillation (AF). Materials and methods. In total, 2423 patients aged from 18 to 94 years (mean age – 57.9 ± 16.4 years), 51 % men, with pathologies of the cardiovascular and respiratory systems and patients without diagnosed diseases of cardiovascular system were enrolled in the study. Echocardiography was performed on an Esaote MyLab Seven device (Italy) according to generally accepted rules. The indicators of systolic and diastolic, valvular functions, the distribution of patients according to four classic types of the left ventricular geometry were studied. Statistical analysis was performed using the Statistica 13.0 software package for Windows. Statistically significant differences were calculated using the Mann–Whitney U test, Pearson’s χ2 test, Kruskal–Wallis test. A level of p < 0.05 was taken to indicate statistical significance. Results. The prevalence of AF in the group of normal geometry was 6.5 %, concentric remodeling – 11.8 %, eccentric hypertrophy – 17.4 %, concentric hypertrophy – 21.7 %. Left ventricular hypertrophy was diagnosed in 56.2 % of patients with AF (32.0 % – eccentric hypertrophy, 27.8 % – concentric hypertrophy), while in the patient group without AF, left ventricular hypertrophy was detected in only 33.9 % of the examined (20.4 % – eccentric hypertrophy, 13.5 % – concentric hypertrophy). In groups of concentric and eccentric hypertrophy, the patients were older, there was a higher prevalence of mitral, aortic, tricuspid valve regurgitation, and lower indicators of systolic function (EF, TEI, S’), diastolic function (e’med) than those in groups with normal geometry and concentric remodeling. In 29.3 % of patients with AF, the geometry of the left ventricle remained normal. Conclusions. The prevalence of AF increased according to the geometric patterns of the left ventricular remodeling with the highest rates in the groups of eccentric and concentric hypertrophy, which were also associated with worse indicators of systolic, diastolic, and valve functions.","PeriodicalId":23785,"journal":{"name":"Zaporožskij Medicinskij Žurnal","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135470896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-28DOI: 10.14739/2310-1210.2023.5.283612
V. Y. Kresiun, N. Al-Nadawi Javad
The development of pharmacological methods to control oxidant stress manifestations in diabetes can be achieved using organic complex germanium compounds. One of the promising compounds is niacin-oxyethylenediphosphonategermanate (MIGU-4), which is an effective corrector of lipid metabolism and a stabilizer of the lipid layer of erythrocyte and hepatocyte membranes in the streptozotocin-induced diabetic model. The aim of the work is to determine the dynamics of the malondialdehyde (MDA) content, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPO) in the brain tissue, as well as the diene conjugate (DC) content, lipid hydroperoxides (LHP), reduced glutathione (GSH) in the liver mitochondria, the serum activities of aspartate and alanine aminotransferases (AST and ALT) in experimental diabetes mellitus with the correction using a complex compound of germanium with nicotinic acid – MIGU-4 and insulin, insulin alone as well as in comparison with effectiveness of vitamin E supplementation. Materials and methods. Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin (60.0 mg/kg). MIGU-4 was administered intraperitoneally at a dose of ED50 that was 25.0 mg/kg. Mitochondrial membranes were obtained by differential centrifugation of the liver tissue. In rats with confirmed diabetes, parameters of peroxidation and antioxidant protection were studied by generally accepted biochemical and biophysical methods. Results. Glucose levels were 29.4 % lower in rats treated with MIGU-4 (25.0 mg/kg) after 8 weeks of streptozotocin-induced diabetes than those in untreated diabetic rats (p < 0.05), while a decrease was 39.0 % (p < 0.05) upon insulin treatment, and 47.2 % (p < 0.05) – with combined use of the drugs. The MDA content in the brain tissue was 3.48 times higher than that in the control (p < 0.05). At the same time, the activities of SOD and CAT were decreased by 46.4 % and 32.0 %, respectively, the activity of GPO was decreased by half (p < 0.05). In the liver mitochondria, the DC content exceeded that in the control by 53.5 % (p < 0.05), and the MDA and LHP levels were 2.48 times and 31.7 % higher (p < 0.05), respectively. AST activity was almost doubled, ALT activity was 5.48 times increased. Insulin and the biologically active substance MIGU-4 used alone exerted a moderate corrective effect. The combined use of the drugs caused definite therapeutic and preventive effects as the brain content of MDA was 2.4 times decreased, the activity of SOD, CAT and GPO was increased by 45.6 %, 35.2 % and 67.3 % (p < 0.05), respectively. In the liver mitochondria, the DC, MDA, and LHP contents were decreased by 41.0 %, 53.3 %, and 28.4 %, respectively, compared to those in rats with diabetes (p < 0.05). The activity of SOD and CAT as well as GSH content were increased by 2.7 times, 51.9 %, and 23.0 % (p < 0.05), respectively. AST and ALT activities were 42.2 % and 74.3 % (p < 0.05) reduced, respect
{"title":"State of peroxidation in the brain and liver in experimental diabetes and its correction possibility with niacin-oxyethylendiphosphonatogermanate","authors":"V. Y. Kresiun, N. Al-Nadawi Javad","doi":"10.14739/2310-1210.2023.5.283612","DOIUrl":"https://doi.org/10.14739/2310-1210.2023.5.283612","url":null,"abstract":"The development of pharmacological methods to control oxidant stress manifestations in diabetes can be achieved using organic complex germanium compounds. One of the promising compounds is niacin-oxyethylenediphosphonategermanate (MIGU-4), which is an effective corrector of lipid metabolism and a stabilizer of the lipid layer of erythrocyte and hepatocyte membranes in the streptozotocin-induced diabetic model. The aim of the work is to determine the dynamics of the malondialdehyde (MDA) content, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPO) in the brain tissue, as well as the diene conjugate (DC) content, lipid hydroperoxides (LHP), reduced glutathione (GSH) in the liver mitochondria, the serum activities of aspartate and alanine aminotransferases (AST and ALT) in experimental diabetes mellitus with the correction using a complex compound of germanium with nicotinic acid – MIGU-4 and insulin, insulin alone as well as in comparison with effectiveness of vitamin E supplementation. Materials and methods. Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin (60.0 mg/kg). MIGU-4 was administered intraperitoneally at a dose of ED50 that was 25.0 mg/kg. Mitochondrial membranes were obtained by differential centrifugation of the liver tissue. In rats with confirmed diabetes, parameters of peroxidation and antioxidant protection were studied by generally accepted biochemical and biophysical methods. Results. Glucose levels were 29.4 % lower in rats treated with MIGU-4 (25.0 mg/kg) after 8 weeks of streptozotocin-induced diabetes than those in untreated diabetic rats (p < 0.05), while a decrease was 39.0 % (p < 0.05) upon insulin treatment, and 47.2 % (p < 0.05) – with combined use of the drugs. The MDA content in the brain tissue was 3.48 times higher than that in the control (p < 0.05). At the same time, the activities of SOD and CAT were decreased by 46.4 % and 32.0 %, respectively, the activity of GPO was decreased by half (p < 0.05). In the liver mitochondria, the DC content exceeded that in the control by 53.5 % (p < 0.05), and the MDA and LHP levels were 2.48 times and 31.7 % higher (p < 0.05), respectively. AST activity was almost doubled, ALT activity was 5.48 times increased. Insulin and the biologically active substance MIGU-4 used alone exerted a moderate corrective effect. The combined use of the drugs caused definite therapeutic and preventive effects as the brain content of MDA was 2.4 times decreased, the activity of SOD, CAT and GPO was increased by 45.6 %, 35.2 % and 67.3 % (p < 0.05), respectively. In the liver mitochondria, the DC, MDA, and LHP contents were decreased by 41.0 %, 53.3 %, and 28.4 %, respectively, compared to those in rats with diabetes (p < 0.05). The activity of SOD and CAT as well as GSH content were increased by 2.7 times, 51.9 %, and 23.0 % (p < 0.05), respectively. AST and ALT activities were 42.2 % and 74.3 % (p < 0.05) reduced, respect","PeriodicalId":23785,"journal":{"name":"Zaporožskij Medicinskij Žurnal","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135470900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-28DOI: 10.14739/2310-1210.2023.5.285114
O. H. Kotenko, A. O. Matviienkiv, M. S. Hryhorian, A. A. Minich, I. O. Kotenko, O. S. Mykhailiuk
Sinusoidal obstruction syndrome (SOS), previously known as hepatic veno-occlusive disease, is manifested by obliterating inflammation of the terminal hepatic veins, characterized by hepatomegaly, right upper quadrant pain, jaundice and ascites, and most often occurs in patients after hematopoietic stem cell transplantation and usually in those who received platinum-based drugs. Cases of SOS development in patients after transplantation of solid organs (lungs, pancreas, liver) are also reported in the world literature. These incidents are rare, and isolated and poorly studied after living-related liver lobe transplantation. The diagnosis is based on clinical signs, imaging techniques (according to ultrasound and radiological methods of examination), histological assessment of liver biopsy. Tacrolimus has been reported to be a causative agent that potentially plays a role in the pathophysiological mechanism of SOS. Aim. To study the relationship between the use of prolonged-release tacrolimus and the development of SOS in patients after living-related liver transplantation. Clinical case. In this article, we present a case of SOS after living-related liver transplantation which was associated with a toxic effect of prolonged-release tacrolimus (“Envarsus”). In a 55-year-old man, after living-related liver transplantation, high blood concentrations of tacrolimus associated with uncontrolled drug intake were detected. When performing a number of laboratory and instrumental methods of examination due to a massive ascites manifestation, the diagnosis of SOS was made. The study was carried out in accordance with the principles of the Helsinki Declaration. The informed consent was obtained from the patient for conducting the studies. Conclusions. By ruling out other possible contributing factors, including an acute rejection crisis, it was concluded that prolonged-release tacrolimus (“Envarsus”) was the cause of SOS.
{"title":"Tacrolimus-associated sinusoidal obstruction syndrome after living-related liver transplantation","authors":"O. H. Kotenko, A. O. Matviienkiv, M. S. Hryhorian, A. A. Minich, I. O. Kotenko, O. S. Mykhailiuk","doi":"10.14739/2310-1210.2023.5.285114","DOIUrl":"https://doi.org/10.14739/2310-1210.2023.5.285114","url":null,"abstract":"Sinusoidal obstruction syndrome (SOS), previously known as hepatic veno-occlusive disease, is manifested by obliterating inflammation of the terminal hepatic veins, characterized by hepatomegaly, right upper quadrant pain, jaundice and ascites, and most often occurs in patients after hematopoietic stem cell transplantation and usually in those who received platinum-based drugs. Cases of SOS development in patients after transplantation of solid organs (lungs, pancreas, liver) are also reported in the world literature. These incidents are rare, and isolated and poorly studied after living-related liver lobe transplantation. The diagnosis is based on clinical signs, imaging techniques (according to ultrasound and radiological methods of examination), histological assessment of liver biopsy. Tacrolimus has been reported to be a causative agent that potentially plays a role in the pathophysiological mechanism of SOS. Aim. To study the relationship between the use of prolonged-release tacrolimus and the development of SOS in patients after living-related liver transplantation. Clinical case. In this article, we present a case of SOS after living-related liver transplantation which was associated with a toxic effect of prolonged-release tacrolimus (“Envarsus”). In a 55-year-old man, after living-related liver transplantation, high blood concentrations of tacrolimus associated with uncontrolled drug intake were detected. When performing a number of laboratory and instrumental methods of examination due to a massive ascites manifestation, the diagnosis of SOS was made. The study was carried out in accordance with the principles of the Helsinki Declaration. The informed consent was obtained from the patient for conducting the studies. Conclusions. By ruling out other possible contributing factors, including an acute rejection crisis, it was concluded that prolonged-release tacrolimus (“Envarsus”) was the cause of SOS.","PeriodicalId":23785,"journal":{"name":"Zaporožskij Medicinskij Žurnal","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135470894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-28DOI: 10.14739/2310-1210.2023.5.277452
O. V. Riabokon, O. O. Furyk, K. V. Kalashnyk
Aim. To analyze a case of severe COVID-19 and influenza co-infection in a 48-year-old female patient. Materials and methods. The clinical course, specifics of diagnosis and therapy of the 48-year-old patient S., who was treated for severe co-infection of COVID-19 and influenza at Municipal non-profit Enterprise “Regional Infectious Clinical Hospital” Zaporizhzhіa Regional Council from 29.01.2023 to 17.02.2023, were analyzed. The diagnosis of COVID-19 was confirmed by the detection of SARS-CoV-2 Ag in nasopharyngeal swabs by the immunochromatographic method, and the diagnosis of influenza – by the influenza A viral RNA detection by the polymerase chain reaction method. Current regulatory documents were used when examining and treating the patient. Results. It has been recognized that the unvaccinated 48-year-old female patient was at a risk group due to comorbid pathology (stage 2 hypertension, type 2 diabetes mellitus, grade 2 obesity) developed a severe course of COVID-19 and influenza type A co-infection. The course of co-infection was complicated by severe acute respiratory distress syndrome already on the 6th day of the disease. The diagnosis of COVID-19 was confirmed by a rapid SARS-CoV-2 antigen test. Treatment for COVID-19 was started immediately with the use of remdesivir, dexamethasone, and anticoagulants. However, despite the patient was given the treatment, his condition worsened due to the rapid progression of acute respiratory failure. The presence of clear clinical and laboratory signs of “cytokine storm” required the use of tocilizumab on the 7th day of the disease. Oseltamivir was commenced after receiving laboratory confirmation of influenza A by the PCR method on the 8th day of the disease. A complete etiologic interpretation of the diagnosis made it possible to prescribe a combination antiviral treatment which coupled with the timely additional initiation of tocilizumab, allowed obtaining certain positive dynamics after only five days of treatment with further improvement of the patient’s condition. Conclusions. Our clinical observation has demonstrated the severe course of COVID-19 and influenza co-infection in the unvaccinated high-risk 48-year-old female patient due to the presence of comorbid pathology. The complete etiologic interpretation of the co-infection has made it possible to prescribe the combination antiviral treatment, which coupled with the additional correction of the immunotropic treatment has enabled to obtain the positive dynamics after only 5 days with further improvement of the patient’s condition
{"title":"A case of severe COVID-19 and influenza co-infection","authors":"O. V. Riabokon, O. O. Furyk, K. V. Kalashnyk","doi":"10.14739/2310-1210.2023.5.277452","DOIUrl":"https://doi.org/10.14739/2310-1210.2023.5.277452","url":null,"abstract":"Aim. To analyze a case of severe COVID-19 and influenza co-infection in a 48-year-old female patient. Materials and methods. The clinical course, specifics of diagnosis and therapy of the 48-year-old patient S., who was treated for severe co-infection of COVID-19 and influenza at Municipal non-profit Enterprise “Regional Infectious Clinical Hospital” Zaporizhzhіa Regional Council from 29.01.2023 to 17.02.2023, were analyzed. The diagnosis of COVID-19 was confirmed by the detection of SARS-CoV-2 Ag in nasopharyngeal swabs by the immunochromatographic method, and the diagnosis of influenza – by the influenza A viral RNA detection by the polymerase chain reaction method. Current regulatory documents were used when examining and treating the patient. Results. It has been recognized that the unvaccinated 48-year-old female patient was at a risk group due to comorbid pathology (stage 2 hypertension, type 2 diabetes mellitus, grade 2 obesity) developed a severe course of COVID-19 and influenza type A co-infection. The course of co-infection was complicated by severe acute respiratory distress syndrome already on the 6th day of the disease. The diagnosis of COVID-19 was confirmed by a rapid SARS-CoV-2 antigen test. Treatment for COVID-19 was started immediately with the use of remdesivir, dexamethasone, and anticoagulants. However, despite the patient was given the treatment, his condition worsened due to the rapid progression of acute respiratory failure. The presence of clear clinical and laboratory signs of “cytokine storm” required the use of tocilizumab on the 7th day of the disease. Oseltamivir was commenced after receiving laboratory confirmation of influenza A by the PCR method on the 8th day of the disease. A complete etiologic interpretation of the diagnosis made it possible to prescribe a combination antiviral treatment which coupled with the timely additional initiation of tocilizumab, allowed obtaining certain positive dynamics after only five days of treatment with further improvement of the patient’s condition. Conclusions. Our clinical observation has demonstrated the severe course of COVID-19 and influenza co-infection in the unvaccinated high-risk 48-year-old female patient due to the presence of comorbid pathology. The complete etiologic interpretation of the co-infection has made it possible to prescribe the combination antiviral treatment, which coupled with the additional correction of the immunotropic treatment has enabled to obtain the positive dynamics after only 5 days with further improvement of the patient’s condition","PeriodicalId":23785,"journal":{"name":"Zaporožskij Medicinskij Žurnal","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135469714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-28DOI: 10.14739/2310-1210.2023.5.279178
D. Ya. Maksymov, O. M. Mishchenko
Aim: to evaluate the intensity of the gingival inflammatory component in the peri-implant area by studying the mucous membrane bleeding in the implant area according to the papilla bleeding index (PBI), when using healing abutments with a surface modified with silver nanoparticles (AgNP). Materials and methods. 40 patients undergoing rehabilitation for secondary partial adentia by the method of dental implantation were enrolled in the study and divided into 2 groups: Group 1 (20 patients) – healing abutments with the surface modified by the method of plasma electrooxidation and AgNP-doped were fixed; Group 2 (20 patients) – standard healing abutments of the implant system used with a polished surface were fixed. The control group (20 patients) – to determine reference values. A total of 60 patients were involved in the study. The degree of bleeding was measured according to the PBI. The mucous membrane thickness was assessed using an endodontic reamer. Results. There was no statistically significant difference in bleeding level 0 between Groups 1 and 2, but the study group was significantly different from the control group (p < 0.001). According to the bleeding level 3, there was a statistically significant difference between Groups 1 and 2, the study group was (12.7 %) versus 48.5 % (p < 0.001). In addition, the study group was significantly different from the control group (p = 0.019), but this was due to the fact that the measurements were taken directly from the wound surface and the comparison was made to understand inflammatory manifestations as a whole. According to bleeding level 4, there were no patients with this level in the study group, but in the comparison group there were 19.2 % of those (p < 0.001). Based on the results of the data obtained, it is advisable to consider effective use of the antibacterial effect of silver nanoparticles to reduce the gingival inflammatory component in the peri-implant area. Conclusions. The use of the bleeding index (PBI) is appropriate in the clinical diagnosis of inflammatory processes in the peri-implant area. Reduction of levels 3, 4 bleeding in the peri-implant area by 35.8 % and 19.2 %, respectively, in patients using healing abutments coated with AgNP compared to polished ones, indicates a pronounced anti-inflammatory effect of the proposed AgNP-doped surface. The absence of a statistical difference in the mucous membrane thickness of the studied areas between the groups allows us to consider the obtained results as consequences of the silver nanoparticle impact with an antibacterial effect. The result of this study allows recommending the proposed AgNP-doped surface for use in clinical practice.
{"title":"The effect of surface-modified silver nanoparticles on the inflammatory component of the intragingival peri-implant area","authors":"D. Ya. Maksymov, O. M. Mishchenko","doi":"10.14739/2310-1210.2023.5.279178","DOIUrl":"https://doi.org/10.14739/2310-1210.2023.5.279178","url":null,"abstract":"Aim: to evaluate the intensity of the gingival inflammatory component in the peri-implant area by studying the mucous membrane bleeding in the implant area according to the papilla bleeding index (PBI), when using healing abutments with a surface modified with silver nanoparticles (AgNP). Materials and methods. 40 patients undergoing rehabilitation for secondary partial adentia by the method of dental implantation were enrolled in the study and divided into 2 groups: Group 1 (20 patients) – healing abutments with the surface modified by the method of plasma electrooxidation and AgNP-doped were fixed; Group 2 (20 patients) – standard healing abutments of the implant system used with a polished surface were fixed. The control group (20 patients) – to determine reference values. A total of 60 patients were involved in the study. The degree of bleeding was measured according to the PBI. The mucous membrane thickness was assessed using an endodontic reamer. Results. There was no statistically significant difference in bleeding level 0 between Groups 1 and 2, but the study group was significantly different from the control group (p < 0.001). According to the bleeding level 3, there was a statistically significant difference between Groups 1 and 2, the study group was (12.7 %) versus 48.5 % (p < 0.001). In addition, the study group was significantly different from the control group (p = 0.019), but this was due to the fact that the measurements were taken directly from the wound surface and the comparison was made to understand inflammatory manifestations as a whole. According to bleeding level 4, there were no patients with this level in the study group, but in the comparison group there were 19.2 % of those (p < 0.001). Based on the results of the data obtained, it is advisable to consider effective use of the antibacterial effect of silver nanoparticles to reduce the gingival inflammatory component in the peri-implant area. Conclusions. The use of the bleeding index (PBI) is appropriate in the clinical diagnosis of inflammatory processes in the peri-implant area. Reduction of levels 3, 4 bleeding in the peri-implant area by 35.8 % and 19.2 %, respectively, in patients using healing abutments coated with AgNP compared to polished ones, indicates a pronounced anti-inflammatory effect of the proposed AgNP-doped surface. The absence of a statistical difference in the mucous membrane thickness of the studied areas between the groups allows us to consider the obtained results as consequences of the silver nanoparticle impact with an antibacterial effect. The result of this study allows recommending the proposed AgNP-doped surface for use in clinical practice.","PeriodicalId":23785,"journal":{"name":"Zaporožskij Medicinskij Žurnal","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135469716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-28DOI: 10.14739/2310-1210.2023.5.278878
M. O. Semen, O. L. Lychkovska, M. Ya. Tyrkus, D. V. Kaminskyy, O. P. Yelisyeyeva
The aim of this study was to examine the peculiarities of trait anxiety and heart rate variability parameters as well as their relationship depending on the catechol O-methyltransferase (COMT) gene Val158Met polymorphism in children with irritable bowel syndrome. Materials and methods. A total of 26 children aged 6–12 years with a verified diagnosis of irritable bowel syndrome according to the Rome IV criteria were examined. Beforehand, all the patients underwent molecular genetic testing for the COMT Val158met single nucleotide polymorphism by using the polymerase chain reaction-restriction fragment length polymorphism method. Heart rate variability was analyzed via cardiointervalography (Neurosoft) based on short five-minute resting-state ECG recordings. The CMAS (Children’s Manifest Anxiety Scale) test was used to measure trait anxiety levels. The Pearson’s test was used to assess correlations between heart rate variability parameters and anxiety levels. Data were processed using Microsoft Excel 2016 and analyzed with GraphPad (Prism 5.0). Results. Depending on the functional COMT Val158met polymorphism, all the patients were allocated to 3 groups: 8 children with 472 GA (Val/Met) COMT genotype; 10 children with 472 AA (Met/Met) genotype; 8 children with 472 GG (Val/Val) genotype. Time and frequency domain parameters of heart rate variability were significantly different in each group. The highest level of anxiety and the largest percentage of LF component (mainly sympathetic activity) in the structure of heart rate variability was noted among Met/Met carriers. Val/Val carriers had a significantly lower anxiety level and an autonomic imbalance with a higher percentage of HF component (parasympathetic activity). Positive correlations between trait anxiety and heart rate variability parameters were found only in Val/Val and Val/Met groups. Conclusions. Our study has revealed the influence of the COMT Val158met polymorphism on the level of trait anxiety and heart rate variability parameters. It is important for a better understanding of the gut-brain axis dysregulation and impaired stress resilience in children with irritable bowel syndrome. Also, these data could be used to improve current schemes for the treatment of irritable bowel syndrome, supplementing them with activation therapy, psychotherapy, psychopharmacotherapy.
本研究的目的是研究肠易激综合征儿童特质焦虑和心率变异性参数的特殊性，以及它们与儿茶酚o -甲基转移酶(COMT)基因Val158Met多态性的关系。材料和方法。共有26名6-12岁的儿童，根据罗马IV标准确诊为肠易激综合征。在此之前，所有患者均采用聚合酶链反应-限制性片段长度多态性方法对COMT Val158met单核苷酸多态性进行分子遗传学检测。基于5分钟静息状态心电图记录，通过心脏间期图(Neurosoft)分析心率变异性。采用CMAS (Children’s Manifest Anxiety Scale)测验测量特质焦虑水平。Pearson检验用于评估心率变异性参数与焦虑水平之间的相关性。数据处理采用Microsoft Excel 2016，分析采用GraphPad (Prism 5.0)软件。结果。根据COMT Val158met多态性的不同，将所有患者分为3组:472 GA (Val/Met) COMT基因型患儿8例;472 AA (Met/Met)基因型患儿10例;472 GG (Val/Val)基因型患儿8例。各组心率变异性时频域参数差异有统计学意义。在Met/Met携带者中，心率变异性结构中焦虑水平最高，LF成分(主要是交感神经活动)比例最大。Val/Val携带者焦虑水平显著降低，自主神经失衡，HF成分(副交感神经活动)百分比较高。特质焦虑与心率变异性参数仅在Val/Val组和Val/Met组中存在正相关。结论。我们的研究揭示了COMT Val158met多态性对特质焦虑水平和心率变异性参数的影响。这对于更好地理解肠-脑轴失调和肠易激综合征儿童的应激恢复能力受损是很重要的。此外，这些数据可以用来改善目前治疗肠易激综合征的方案，补充激活疗法，心理疗法，精神药物疗法。
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