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SEAWEED TISSUE CULTURE TECHNIQUE IN BALAI PERIKANAN BUDIDAYA AIR PAYAU (BPBAP) SITUBONDO
Pub Date : 2022-03-01 DOI: 10.31002/jade.v5i1.5474
Lusiana BR Ritonga
Kappapyhcus alvarezii seaweed or more commonly known as Eucheuma cottonii is a potential commodity to be developed because seaweed has many benefits. The problem found in the development of seaweed in Indonesia is the number of pests and diseases in seaweed, especially ice-ice. In vitro culture techniques have become an alternative to help overcome the problems that are often faced in the provision of seeds through conventional means. There are 6 stages in seaweed tissue culture, namely broodstock acclimatization, callus induction, callus/micropropagule regeneration, micropropagule regeneration, plantlet acclimatization and seed propagation in the sea. During the observation, water quality was still in the normal range, namely temperatures ranging from 25-30 0C, pH between 8.16-8.35 and salinity 34-36 ppt.
Kappapyhcus alvarezii海藻或更常见的Eucheuma cottonii是一种潜在的商品,因为海藻有许多好处。在印度尼西亚海藻的发展中发现的问题是海藻的病虫害数量,特别是海冰。体外培养技术已成为帮助克服通过传统手段提供种子时经常面临的问题的一种替代方法。海藻组织培养过程分为6个阶段,即亲本驯化、愈伤组织诱导、愈伤组织/微繁殖体再生、微繁殖体再生、植株驯化和种子在海中繁殖。在观察期间,水质仍处于正常范围,即温度为25-30℃,pH为8.16-8.35,盐度为34-36 ppt。
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引用次数: 0
Abundance of Phytoplankton in Vannamei Shrimp (Litopenaeus vannamei) Farming Pond at the Center for Brackish Water Aquaculture Fisheries (BBPBAP) Jepara Jepara咸淡水养殖渔业中心(BBPBAP)凡纳美对虾养殖池浮游植物丰度
Pub Date : 2022-03-01 DOI: 10.31002/jade.v5i1.5678
A. Asmarany
Vannamei shrimp (Litopenaeus vannamei) is one of the commodities that is a priority in aquaculture development activities in Indonesia. The success of vannamei shrimp culture is influenced by various factors such as fry quality, feed quality, land carrying capacity, application of biosecurity and good water quality management. Water quality is closely related to the phytoplankton community contained in a waters. This study aims to analyze the abundance and dominance of plankton species found in pond waters. Monitoring the abundance of phytoplankton was carried out in 4 plots of vannamei shrimp aquaculture ponds at the Brackish Water Aquaculture Fisheries Center (BBPBAP) Jepara, namely plots A51, A51, A61 and A62. Identification and calculation of phytoplankton cells were carried out using a haemocytometer and Sedgwick. The results of the analysis showed that the highest abundance of phytoplankton was found in plots A61 at week 9 with a cell number of 36.73 x 106 cells/L, while plots A51, A52 and A62 decreased. Overall plot A62 had better water quality conditions when compared to plots A51, A52 and A61 indicated by the phytoplankton abundance value of 34.63 x 106 cells/L at week 9 and the dominance index of green algae, namely D > 0.5.
凡纳美对虾(凡纳美对虾)是印度尼西亚水产养殖发展活动中的优先商品之一。凡纳美对虾养殖的成功受多种因素的影响,如鱼苗质量、饲料质量、土地承载能力、生物安全措施的应用和良好的水质管理。水质与水体中浮游植物群落密切相关。本研究旨在分析池塘水域浮游生物种类的丰度和优势度。在Jepara咸淡水养殖渔业中心(BBPBAP)的4个样地凡纳美对虾养殖池进行了浮游植物丰度监测,分别为A51、A51、A61和A62样地。利用血细胞计和塞奇威克进行浮游植物细胞的鉴定和计算。分析结果表明,第9周,A61样地浮游植物丰度最高,细胞数为36.73 × 106个/L,而A51、A52和A62样地浮游植物丰度下降。从第9周浮游植物丰度值为34.63 × 106 cells/L,绿藻优势度指数D > 0.5来看,总体上A62地块的水质条件较A51、A52和A61地块好。
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引用次数: 0
Identification of Ectoparasite Infection in Tilapia (Oreochromis niloticus) Based on Water Quality in Rawa Pening 基于拉瓦坪水质的罗非鱼体外寄生虫感染鉴定
Pub Date : 2022-03-01 DOI: 10.31002/jade.v5i1.6213
Tholibah Mujtahidah, S. Hidayati, N. Ambarwati
Testing of ectoparasites on water quality in infections that occured in tilapia in aquaculture activities in Rawa Pening. The purpose of this study was to determine the exposure to the abundance of ectoparasite bacteria that infected the growth of tilapia in Rawa Pening. This study used experimental method and purposive sampling with data processing in a descriptive survey. The data analysis used was analysis of variance (ANOVA) with the aim of knowing the effect of sampling and the parameters tested, this test was carried out 4 (four) times for water quality and ectoparasite observations 3 (three) times for testing to obtain the accuracy of data sampling. The results of this study showed that there was an infection with levels of ectoparasites identified from the types of Streptococcus sp, Lernaea sp, Aeromonas sp, and Trichodina sp. These bacteria attack fish with the highest number or total exposure in the Tuntang area. The reason was, currently water hyacinth was being cut down as one of the local government programs to reduce the blooming of water hyacinth (Eichhornia crassipes) of 7.92 mg/L, pH levels 7.59, ammonia levels 10-20 mg/L and nitrate levels 0.5 mg/L were still in optimal conditions from the results carried out.
拉瓦坪养殖活动中罗非鱼感染的水质外寄生虫检测。本研究的目的是确定暴露于感染罗非鱼生长的体外寄生虫细菌的丰度。本研究采用实验法和有目的抽样,资料处理为描述性调查。数据分析采用方差分析(ANOVA),目的是了解采样和测试参数的影响,该检验对水质和体外寄生虫观测进行了4(4)次检验,3(3)次检验,以获得数据采样的准确性。本研究结果表明,屯塘地区存在感染外寄生虫的情况,主要有链球菌、勒氏杆菌、气单胞菌和毛癣菌,这些细菌攻击鱼类的数量或总暴露量在屯塘地区最高。原因是,目前水葫芦被当地政府列为减少水葫芦(Eichhornia crassipes)开花的项目之一,从实施结果来看,7.92 mg/L、pH值7.59、氨浓度10-20 mg/L、硝酸盐浓度0.5 mg/L仍处于最佳状态。
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引用次数: 0
Application of The Temperature Shock Method (Heat Shock) on Pearl Oyster (Pinctada maxima) Hatcheries 温度冲击法(Heat Shock)在珍珠贝孵化场的应用
Pub Date : 2022-03-01 DOI: 10.31002/jade.v5i1.5666
Lusiana BR Ritonga
The low production and quality of pearls produced from the cultivation process, one of which is caused by the less than optimal quality of pearl oysters. Spawning is a determining factor in hatchery activities. Therefore, in an effort to increase production, it requires strategies and improvements in the pearl oyster spawning process. This study aims to apply the temperature shock method as an effort to increase productivity and success rate of pearl oyster hatchery. The results of observations on gonad maturity in pearl oyster broodstock obtained data from a total of 200 broods consisting of 135 male broods and 75 female brooders aged 3.5 years, the percentage of perfectly gonadally mature broodstock was 11.85% and 12%, respectively a total of 25 individuals. The results of spawning pearl oyster broodstock through the temperature shock method of a total of 18 broods observed were 12 brooders or 66.7% which spawned perfectly. The results of the observation of the spawning process of pearl oyster broodstock showed that from 8 female broodstock ± 90,000,000 eggs were obtained with a hatching rate of 70% with a spat harvest of ± 1,575,000 individuals or 2.5% of the total hatched larvae final yield.
养殖过程中产生的珍珠产量和质量低,其中一个原因是珍珠牡蛎的质量不理想。产卵是孵化场活动的决定因素。因此,为了提高产量,需要在珍珠牡蛎产卵过程中采取策略和改进措施。本研究旨在应用温度冲击法提高珍珠贝孵化场的产量和成功率。对35岁以上的珠蚌种鱼进行了性腺成熟的观察,其中雄种鱼135条,雌种鱼75条,共200条,性腺完全成熟的比例分别为11.85%和12%,共25条。温度冲击法对18条珠蚌进行产卵试验,结果显示,12条珠蚌产卵成功率为66.7%。对珍珠贝产卵过程的观察结果表明,8条母贝共产卵约9000万枚,孵化率为70%,产卵约157.5万枚,占总孵化量的2.5%。
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引用次数: 0
CHARACTERISTICS OF MALONG (Muraenesox cinereus) AND KEMBUNG (Rastrelliger brachysoma) AS SURIMI RAW MATERIAL 马龙(Muraenesox cinereus)和KEMBUNG (Rastrelliger brachysoma)作为鱼糜原料的特性
Pub Date : 2022-03-01 DOI: 10.31002/jade.v5i1.6212
Andri Nofreeana, Untung Trimo Laksono
The use of fish as raw material for surimi continued to grow along with the increasing demand for surimi-based processed products. The fish used from marine fish to freshwater fish. The use of fresh fish as raw material greatly affected the quality of surimi. The morphological characteristics of fish raw materials affected the yield of fish meat, this can be seen in the yield of each fish. This research used two types of fish, namely Malong / Daggertooth pike conger fish (Muraenesox cinereus) and Kembung / Mackerel fish (Rastrelliger brachysoma). In general, the results of the proximate test showed that the protein levels in the fish used were not significant. Malong fish was very potential to be used as raw material for surimi because it produced the highest yield, which was 66.67% with 62.44% myofibril and 25.6% sarcoplasm. Kembung/Mackerel fish in this study resulted in the lowest yield of 37.00% with 59.69% of myofibril and 46.6% of sarcoplasm. Gel formation in surimi was strongly influenced by myofibril and sarcoplasmic proteins. Gel formation in fish meat was strongly influenced by the presence of salt-soluble proteins in the form of myofibrils (actin and myosin). On the other hand, if fish meat contained more water-soluble protein (sarcoplasm), it would be relatively reduce its gel formation ability.
随着对以鱼为基础的加工产品的需求不断增加,鱼作为鱼糜原料的使用继续增长。所用的鱼类从海鱼到淡水鱼。鲜鱼作为原料的使用极大地影响了鱼糜的质量。鱼类原料的形态特征影响了鱼肉的产量,这可以从每条鱼的产量中看出。本研究使用了两种鱼类,即龙/匕首牙梭子鱼(Muraenesox cinereus)和Kembung /鲭鱼(Rastrelliger brachysoma)。一般来说,近似试验的结果表明,所用鱼的蛋白质水平并不显著。马龙鱼的产率最高,为66.67%,肌原纤维含量为62.44%,肌质含量为25.6%,具有很大的开发潜力。本研究的Kembung/鲭鱼的产量最低,为37.00%,肌原纤维含量为59.69%,肌质含量为46.6%。鱼糜的凝胶形成受到肌原纤维和肌浆蛋白的强烈影响。鱼肉中的凝胶形成受到以肌原纤维形式存在的盐溶性蛋白质(肌动蛋白和肌球蛋白)的强烈影响。另一方面,如果鱼肉中含有较多的水溶性蛋白(肌浆),则会相对降低其成胶能力。
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引用次数: 0
SEAWEED AS A DIETARY FIBER TO PREVENT THE CARDIOVASCULAR DISEASE 海藻作为膳食纤维可以预防心血管疾病
Pub Date : 2022-03-01 DOI: 10.31002/jade.v5i1.6237
Andri Nofreeana, Muhammad Azril
Indonesia has a lot of potential in natural resources, one of which is seaweed. Seaweed is one of the natural ingredients that contain water-soluble dietary fiber. The fiber content in seaweed can be categorized as a functional food. The fiber content in seaweed varies, from 36-60% dry weight, 55-70% of the former percentage is soluble fiber consisting of alginate and carrageenan with varying amounts depending on the type of seaweed and environmental conditions. The dietary fibers are useful for disease prevention and health maintenance. Dietary fiber can prevent several diseases, including coronary heart disease (cardiovascular). The effect of dietary fiber according to Liu et al (2002) is a very high fiber diet (55 g/1,000 kcal) which resulted in a 33% reduction in LDL cholesterol (Low-Density Lipoprotein) in two weeks period. The mechanism of dietary fiber reduces cardiovascular disease since it is able to bind bile acids (the end product of cholesterol), thereby preventing its reabsorption from the intestine. Cholesterol that is bound to bile acids and lignin/pectin cannot be absorbed by the intestines but will be excreted in the feces.
印尼有很多潜在的自然资源,其中之一就是海藻。海藻是一种含有水溶性膳食纤维的天然成分。海藻纤维含量高,可归类为功能性食品。海藻中的纤维含量各不相同,从干重的36-60%,前一个百分比的55-70%是由海藻酸盐和卡拉胶组成的可溶性纤维,其含量取决于海藻的类型和环境条件。膳食纤维对预防疾病和保持健康很有帮助。膳食纤维可以预防几种疾病,包括冠心病(心血管)。根据Liu等人(2002)的研究,膳食纤维的作用是高纤维饮食(55克/ 1000千卡),在两周内导致低密度脂蛋白胆固醇(低密度脂蛋白)降低33%。膳食纤维的机制减少心血管疾病,因为它能够结合胆汁酸(胆固醇的最终产物),从而防止其从肠道重新吸收。与胆汁酸和木质素/果胶结合的胆固醇不能被肠道吸收,而会随粪便排出体外。
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引用次数: 0
Application for Handling Hybrid Grouper Eggs (Epinephelus lanceolatus x Epinephelus fuscoguttatus) 杂交石斑鱼卵(Epinephelus lanceolatus x Epinephelus fuscoguttatus)处理申请书
Pub Date : 2022-03-01 DOI: 10.31002/jade.v5i1.5683
Muh Sulaiman Dadiono, Maheno Sri Widodo
Applications for handling hybrid grouper eggs (Epinephelus lanceolatus x Epinephelus fuscoguttatus) include the initial egg handling process, egg selection, egg counting and egg observation. The initial handling of hybrid grouper eggs (Epinephelus lanceolatus x Epinephelus fuscoguttatus) begins with stripping the female tiger grouper and male giant grouper to get eggs and sperm. Mixing eggs and sperm is done using chicken feathers. Egg selection is done to separate good and bad quality eggs. Good quality eggs will float on the surface, are round, transparent in color and have a core. Poor quality eggs will sink to the bottom and become milky white. The egg calculation technique uses the manual method with the help of a petri dish and a 20 ml beaker glass. Observation of eggs using a microscope magnification 40x. The fertilized egg has a transverse line between the nucleus of the egg which is a developing embryo. The unfertilized egg does not have a transverse line which indicates the egg does not have an embryo.
处理杂交石斑鱼(Epinephelus lanceolatus x Epinephelus fuscoguttatus)卵的应用包括卵的初始处理过程、卵的选择、卵的计数和卵的观察。杂交石斑鱼卵(Epinephelus lanceolatus x Epinephelus fuscoguttatus)的初始处理是从剥去雌性虎石斑鱼和雄性巨石斑鱼的卵和精子开始的。卵子和精子的混合是用鸡毛完成的。鸡蛋的选择是为了区分优质鸡蛋和劣质鸡蛋。好的鸡蛋会浮在水面上,呈圆形,颜色透明,有核。劣质鸡蛋会沉到底部,变成乳白色。鸡蛋计算技术使用人工方法,在培养皿和20毫升烧杯的帮助下。用40倍放大镜观察卵。受精卵的细胞核之间有一条横线,这是一个发育中的胚胎。未受精卵没有横线,这表明卵子没有胚胎。
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引用次数: 2
DIFFERENT FORMS OF CORAL FUNGIA PAUMOTENSIS, IN EACH DIFFERENT LOCATION ON THE ISLAND OF MAMBURIT, SUMENEP DISTRICT 不同形式的珊瑚真菌,在曼布里特岛的每个不同位置,苏梅内普区
Pub Date : 2022-03-01 DOI: 10.31002/jade.v5i1.6036
Sawiya Sawiya, A. Muqsith
Coral morphological characters are mainly caused by environmental factors. The method used is random sampling with one sample, two repetitions at each station. The stations selected were Leeward I, Leeward II, Windward I, and Windward II. Based on the results obtained in the waters of Mamburit, Fungia paumotensis, General physical characteristics included a hilly colony surface, pointed teeth and small grains and curved corals. Morphological characters show differences from discrete and morphometric characters in each sample where these differences are influenced by the environment
珊瑚的形态特征主要受环境因素的影响。所采用的方法是随机抽样,每站一个样本,重复两次。选择的站点是背风1号、背风2号、背风1号和背风2号。根据在Mamburit水域获得的结果,Fungia paumotensis的一般物理特征包括丘陵的菌落表面,尖牙,小颗粒和弯曲的珊瑚。形态学特征在每个样本中显示出离散和形态计量特征的差异,这些差异受到环境的影响
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引用次数: 0
GROWTH MONITORING OF SAND LOBSTER (Panulirus homarus) USING THE BOTTOM CAGE METHOD IN THE EXAMPLE UNIT OF MARINE LOBSTER CULTIVATION, BANYUWANGI REGENCY, EAST JAVA PROVINCE 采用底笼法对东爪哇省半玉望基县海虾养殖示范单位沙龙虾生长监测
Pub Date : 2021-11-01 DOI: 10.31002/jade.v4i2.4794
Asep Akmal Aonullah
With the high demand for sea lobster as the industry has developed, Indonesia has an opportunity to establish the world's largest lobster aquaculture production. Therefore, a study related to the growing-out of lobster and also the development of engineering technology of lobster enlargement is needed. This study aims to determine the growth performance of Panulirus homarus reared in a bottom cage method. Lobsters with an average size of 116,25 g were reared for 150 days and fed with trash fish of 3%-4% of the biomass daily. The results showed that the use of the bottom cage method had positive effects on the survival rate, average body weight, specific growth rate dan food conversion ratio was 85%, 170 g, 0.25 g per day and11.16 respectively. The water quality is also was still within the suitable range for lobster rearing.
随着该产业的发展,对海龙虾的需求很高,印度尼西亚有机会建立世界上最大的龙虾养殖产量。因此,有必要对龙虾的生长发育进行研究,并开发龙虾扩大的工程技术。本研究的目的是确定底笼饲养法饲养的同属蝶鼠的生长性能。平均大小为116,25 g的龙虾饲养150天,每天投喂占生物量3%-4%的垃圾鱼。结果表明,采用底笼法对成活率、平均体重、特定生长率和食物转化率的影响分别为85%、170 g、0.25 g / d和11.16 g / d。水质也处于适宜龙虾养殖的范围内。
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引用次数: 0
Plankton Profile in White Shrimp Pond (L. vannamei) 白对虾池浮游生物剖面
Pub Date : 2021-11-01 DOI: 10.31002/jade.v4i2.4732
H. Ariadi, Dwi Ario Fajar
Plankton are microscopic organisms that exist in the waters of shrimp ponds. The purpose of this study was to determine the profile of plankton dominance in white shrimp (L. vannamei) aquaculture ponds. This research was conducted using an ex-pose facto causal design method in intensive white shrimp culture ponds. The results of this study showed that in the research shrimp ponds, 4 main genera of plankton were found, namely the genus chlorophyceae consisting of species chlamydomonas sp., chlorella sp., oocystis sp., tetracelmis sp., genus cyanophyceae consisting of species anabaena sp., chroococcus sp., microcystis sp., oschillatoria sp., genus Euglenophyceae namely phacus sp. and the genus Dinophyceae which consists of the species gymnodinium sp. noctiluca sp. peridinium sp. prorocentrum sp. Overall the level of dominance of abundance and community dominance index was dominated by plankton of the genus chlorophyceae as much as 1.03E+06 cells/ml and a dominance index of 82.40%. Then from the genus chlorophyceae the dominant species is plankton species chlorella sp., with an abundance of 7.50E+05 cells/ml and a dominance index of 73%. The conclusion of this study is that plankton in the research ponds are dominated by the genus chlorophyceae which consists of dominant species of chlorella sp and chlamydomonas sp, oocystis sp, tetracelmis sp which are predominant.
浮游生物是存在于虾塘水中的微生物。本研究的目的是确定凡纳白对虾养殖池塘浮游生物优势度的分布。本研究采用事实因果设计方法在集约化养殖白虾池中进行。研究结果表明,在研究对虾池中发现了4个主要的浮游生物属,即由衣藻属、小球藻属、卵囊藻属、四鳃藻属组成的绿藻属,由水藻属、绿球藻属、微囊藻属、振荡藻属、裸藻属(phacus sp.)和由裸子藻属(gymnodinium sp.)、夜藻属(noctiluca sp. peridinium sp. proocentrum sp.)组成的藻属(Dinophyceae genus)。总体上,浮游生物的丰度优势水平和群落优势指数均以绿藻属浮游生物为优势,最高达1.03E+06 cells/ml,优势指数为82.40%。绿藻属的优势种为浮游生物小球藻,丰度为7.50E+05 cells/ml,优势度指数为73%。本研究的结论是:研究池浮游生物以绿藻属为主,优势种有小球藻,优势种有衣藻、卵囊藻、四鳃藻。
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引用次数: 0
期刊
Journal of Aquaculture Development and Environment
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