{"title":"Identification and Quantification of Newly Synthesized Proteins Using Mass Spectrometry‐Based Chemical Proteomics","authors":"Suttipong Suttapitugsakul, Haopeng Xiao, Ronghu Wu","doi":"10.1002/9781118970195.ch8","DOIUrl":"https://doi.org/10.1002/9781118970195.ch8","url":null,"abstract":"","PeriodicalId":261030,"journal":{"name":"Mass Spectrometry‐Based Chemical Proteomics","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127722575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-13DOI: 10.1002/9781118970195.ch5
S. Lovell, Charlotte L. Sutherell, E. Tate
{"title":"Chemical Probes for Proteins and Networks","authors":"S. Lovell, Charlotte L. Sutherell, E. Tate","doi":"10.1002/9781118970195.ch5","DOIUrl":"https://doi.org/10.1002/9781118970195.ch5","url":null,"abstract":"","PeriodicalId":261030,"journal":{"name":"Mass Spectrometry‐Based Chemical Proteomics","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116774257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-13DOI: 10.1002/9781118970195.ch3
B. J. Killinger, Kristoffer R. Brandvold, Susan J. Ramos-Hunter, A. Wright
{"title":"Chemoproteomic Analyses by Activity‐Based Protein Profiling","authors":"B. J. Killinger, Kristoffer R. Brandvold, Susan J. Ramos-Hunter, A. Wright","doi":"10.1002/9781118970195.ch3","DOIUrl":"https://doi.org/10.1002/9781118970195.ch3","url":null,"abstract":"","PeriodicalId":261030,"journal":{"name":"Mass Spectrometry‐Based Chemical Proteomics","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130667668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-26DOI: 10.1002/9781118970195.ch11
Sindhuja Sridharan, Ina Günthner, Isabelle Becher, M. Savitski, M. Bantscheff
{"title":"Target Discovery Using Thermal Proteome Profiling","authors":"Sindhuja Sridharan, Ina Günthner, Isabelle Becher, M. Savitski, M. Bantscheff","doi":"10.1002/9781118970195.ch11","DOIUrl":"https://doi.org/10.1002/9781118970195.ch11","url":null,"abstract":"","PeriodicalId":261030,"journal":{"name":"Mass Spectrometry‐Based Chemical Proteomics","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129428782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-26DOI: 10.1002/9781118970195.ch13
K. Suazo, G. Schey, Chad L Schaber, Audrey R. Odom John, M. Distefano
{"title":"Proteomic Analysis of Protein–Lipid Modifications: Significance and Application","authors":"K. Suazo, G. Schey, Chad L Schaber, Audrey R. Odom John, M. Distefano","doi":"10.1002/9781118970195.ch13","DOIUrl":"https://doi.org/10.1002/9781118970195.ch13","url":null,"abstract":"","PeriodicalId":261030,"journal":{"name":"Mass Spectrometry‐Based Chemical Proteomics","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121277212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-26DOI: 10.1002/9781118970195.ch1
Yu Gao, J. Yates
In mass‐spectrometry‐based protein analysis, there are two major strategies, the top‐down method and the bottom‐up method [1, 2]. The terms “top” and “bottom” refer to the complexity of the analyte, namely the more complex “protein” and less complex “peptide.” In top‐down protein analysis, the intact protein is directly analyzed by mass spectrometer. Mass information and fragment ions are generated from the intact protein ions and are then used for direct protein identification and characterization. In comparison, bottom‐up method starts with digesting the protein into peptides by either chemical or enzymatic digestion. The peptide product is then analyzed by a tandem mass spectrometer, and the peptide molecular weight and fragmentation informa tion is matched back to the original protein or protein mixture. When a mix ture of proteins is analyzed by a bottom‐up method, it is also called shotgun proteomics, owing to the similarity to shotgun genomic sequencing.
{"title":"Protein Analysis by Shotgun Proteomics","authors":"Yu Gao, J. Yates","doi":"10.1002/9781118970195.ch1","DOIUrl":"https://doi.org/10.1002/9781118970195.ch1","url":null,"abstract":"In mass‐spectrometry‐based protein analysis, there are two major strategies, the top‐down method and the bottom‐up method [1, 2]. The terms “top” and “bottom” refer to the complexity of the analyte, namely the more complex “protein” and less complex “peptide.” In top‐down protein analysis, the intact protein is directly analyzed by mass spectrometer. Mass information and fragment ions are generated from the intact protein ions and are then used for direct protein identification and characterization. In comparison, bottom‐up method starts with digesting the protein into peptides by either chemical or enzymatic digestion. The peptide product is then analyzed by a tandem mass spectrometer, and the peptide molecular weight and fragmentation informa tion is matched back to the original protein or protein mixture. When a mix ture of proteins is analyzed by a bottom‐up method, it is also called shotgun proteomics, owing to the similarity to shotgun genomic sequencing.","PeriodicalId":261030,"journal":{"name":"Mass Spectrometry‐Based Chemical Proteomics","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121852320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-26DOI: 10.1002/9781118970195.ch4
K. Ruddraraju, Zhon-Yin Zhang
{"title":"Activity‐Based Probes for Profiling Protein Activities","authors":"K. Ruddraraju, Zhon-Yin Zhang","doi":"10.1002/9781118970195.ch4","DOIUrl":"https://doi.org/10.1002/9781118970195.ch4","url":null,"abstract":"","PeriodicalId":261030,"journal":{"name":"Mass Spectrometry‐Based Chemical Proteomics","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130781082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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