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Molecular detection of Babesia microti in one-humped camel (Camelus dromedarius) in Halayeb and Shalateen, Halayeb, Egypt 埃及Halayeb和Shalateen地区单峰骆驼(Camelus dromedarius)微小巴贝斯虫的分子检测
Pub Date : 2021-11-11 DOI: 10.21608/evmspj.2021.204355
M. Rizk
Mohamed Abdo Rizk 1, * Mohamed A. ElAdl , Mostafa Al-Araby , Mayar O. Ali , Shimaa Abd El-Salam El-Sayed , Mohamed El-Beskawy , Naglaa A. Gomaa Addresses 1 Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt 2 National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, Japan 3 Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt 4 Department of Parasitology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt 5 Department of Animal Genetics, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt 6 Department of Internal Medicine (infectious diseases), Faculty of Veterinary Medicine, Matrouh University, Egypt. 7 Department of Animal Medicine, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt. *Corresponding author Mohamed Abdo Rizk, PhD Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. Tel.: +201019474865; Fax: + 20502379952; E-mail address: dr_moh_abdo2008@mans.edu.eg Accepted: January 2021 Abstract
Mohamed Abdo Rizk 1, * Mohamed A. ElAdl, Mostafa Al-Araby, Mayar O. Ali, Shimaa Abd El-Salam El-Sayed, Mohamed El-Beskawy, Naglaa . Gomaa Addresses 1曼苏拉大学兽医学院内科与传染病学系,埃及曼苏拉35516 2日本北海道大比罗市稻田市大比罗农业与兽医大学国立原生动物疾病研究中心3营养生物化学与化学学系,曼苏拉大学兽医学院,曼苏拉35516,埃及4曼苏拉大学兽医学院寄生虫学系,曼苏拉35516,埃及5曼苏拉大学兽医学院动物遗传学系,曼苏拉35516,埃及6马特鲁大学兽医学院内科(传染病)系,埃及7 Kafrelsheikh大学兽医学院动物医学系,埃及Kafrelsheikh,埃及。*通讯作者Mohamed Abdo Rizk博士,曼苏拉大学兽医学院内科和传染病系,埃及曼苏拉35516。电话:+ 201019474865;传真:+ 20502379952;电子邮件地址:dr_moh_abdo2008@mans.edu.eg接收日期:2021年1月
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引用次数: 3
Efficacy of triage parasite panel in diagnosis of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum antigens in symptomatic children stool specimens 分类寄生虫小组对有症状儿童粪便标本中溶组织内阿米巴、兰第鞭毛虫和细小隐孢子虫抗原的诊断效果
Pub Date : 2021-09-01 DOI: 10.21608/evmspj.2021.192801
Gamal AboSheishaa
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引用次数: 1
Efficacy of allicin oil and diclazuril on broiler chickens: Effect on caecal coccidiosis and caecal tissue antioxidant 大蒜素油和地唑利对肉鸡盲肠球虫病及盲肠组织抗氧化的影响
Pub Date : 2021-01-01 DOI: 10.21608/evmspj.2021.297930
Amany Abd-Elghany
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引用次数: 0
First time recording of seasonal prevalence of Cysticercus tenuuicollis among small ruminants at Matrouh City,Egypt. 首次记录埃及马特鲁市小反刍动物中细囊尾蚴季节性流行。
Pub Date : 2020-12-30 DOI: 10.21608/EVMSPJ.2020.142941
Nadia Laban
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引用次数: 0
Prevalence and Phylogenetic analysis of Fasciola species in Upper Egypt Based on Ribosomal ITS-2 gene Sequencing 基于核糖体ITS-2基因测序的上埃及片形吸虫流行病学及系统发育分析
Pub Date : 2020-12-24 DOI: 10.21608/evmspj.2020.132162
Reda Khalafala
142 Ahmed G. Abdelazeem, Amer Ragheb Abdelaziz, Reda E. Khalafalla, Mostafa F.N. Abushahba, 1 Department of Parasitology, Faculty of Veterinary Medicine, Aswan University, Egypt. Department of Parasitology, Faculty of Veterinary Medicine, Sohag University, 82524, Egypt. Department of Parasitology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, 33516, Egypt. Department of Zoonoses, Faculty of Veterinary Medicine, Assiut University, 71526, Egypt. Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO 63110, USA.
142 Ahmed G. Abdelazeem, Amer Ragheb Abdelaziz, Reda E. Khalafalla, Mostafa F.N. Abushahba, 1埃及阿斯万大学兽医学院寄生虫学系。埃及索哈格大学动物医学院寄生虫学教研室,82524Kafrelsheikh大学兽医学院寄生虫学教研室,埃及Kafr El-Sheikh 33516。埃及阿苏特大学动物医学院人畜共患病学系,埃及71526华盛顿大学医学院传染病学部医学系,密苏里州圣路易斯63110
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引用次数: 6
Improved DNA extraction technique from blood clot for diagnosis of Trypanosoma evansi in camels 改进血凝块DNA提取技术诊断骆驼伊凡斯锥虫病
Pub Date : 2020-02-05 DOI: 10.21608/evmspj.2020.71062
Amira W. Deweir
The detection of Trypanosoma evansi genetic material in clinical samples is considered an important diagnostic tool. When blood clots can be a valuable source of genetic information. In our paper, Fourty blood samples were collected from camels at Cairo abbatoir in plane tubes. Blood clots were dispersed by scissor and treated by lysis buffer and proteinase-K, then DNA was extracted according to protocoal of Gene JET Whole Blood Genomic DNA Purification Mini Kit. the quality and integrity of the extracted DNA were corroborated by agarose gel electrophoresis and conventional PCR. Results confirmed that DNA purified from clotted blood can be used in further amplifications. Moreover 16 out of 40 samples (40%) were positive in PCR with bands at the expected molecular weight (164pb). The objective of the present study was to improve and develop a more sensitive method to recover T. evansi DNA from clot samples.
临床样本中伊文氏锥虫遗传物质的检测被认为是一种重要的诊断工具。血凝块可以是遗传信息的宝贵来源。在我们的论文中,从开罗屠宰场的骆驼身上采集了40份血液样本。用剪刀将血凝块分散,用裂解缓冲液和蛋白酶- k处理,然后按照Gene JET全血基因组DNA纯化迷你试剂盒的方案提取DNA。琼脂糖凝胶电泳和常规PCR证实了提取DNA的质量和完整性。结果证实,从凝血中纯化的DNA可用于进一步扩增。此外,40份样品中有16份(40%)PCR阳性,条带达到预期分子量(164pb)。本研究的目的是改进和开发一种更灵敏的方法从血凝块样品中提取伊文氏t细胞DNA。
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引用次数: 0
Parasitological Prevalence of Cryptosporidium infection in calves, chickens and human in Behera province 河北省犊牛、鸡和人隐孢子虫感染的寄生虫学调查
Pub Date : 2019-11-01 DOI: 10.21608/evmspj.2019.69608
Mohamed Bessat
Cryptosporidium is a cosmopolitan intestinal protozoan parasite that belongs to the phylum Apicomplexa and infects a broad range of vertebrate hosts including livestock, human and birds. This current study was aimed to investigate the prevalence of Cryptosporidium spp.infection in young calves, human and chickens, in five districts covering five different geographical locations of the Behera province. In doing this, a total number of 310 faecal samples from calves (n= 148), human being (n=112) and chicken (n=50) were collected, stained using the modified Ziehl-Neelsen staining method, and were examined microscopically for Cryptosporidium spp. oocysts. Correlations between prevalence rates and related parameters such as species, age, gender and faecal consistencies were also analyzed. Results indicated that 43.2%, 16.1% and 6%, of calves, human and chicken, were found infected with Cryptosporidium spp., respectively. Statistically significant variable rates of infection were also detected across six surveyed districts of the Behera governorate, with the notable absence of infection from chicken in Abo Hummus and Rahmaneyah districts. Statistically significant variable rates of infection were also characterized in calves when species, age, sex, and faecal consistency were analyzed; buffalo >4 weeks calves, male and calves with formed faeces have higher infection rates than their counterparts of cattle Cryptosporidium spp. in the livestock and chicken living in close proximity to human population necessitates better surveillance and control measures to protect vulnerable animal and human populations.
隐孢子虫是一种世界性的肠道原生动物寄生虫,属于顶复门,感染广泛的脊椎动物宿主,包括家畜、人类和鸟类。本研究旨在调查隐孢子虫在贝赫拉省5个不同地理位置的5个地区的幼牛、人和鸡的感染情况。本研究共收集犊牛(148例)、人(112例)和鸡(50例)粪便310份,采用改良Ziehl-Neelsen染色法染色,并在显微镜下检测隐孢子虫卵囊。还分析了患病率与相关参数(如物种、年龄、性别和粪便浓度)之间的相关性。结果犊牛、人、鸡隐孢子虫感染率分别为43.2%、16.1%和6%。在贝赫拉省的6个调查区还发现了具有统计意义的可变感染率,Abo Hummus区和Rahmaneyah区没有鸡感染。当分析种类、年龄、性别和粪便一致性时,犊牛的感染变异率也具有统计学意义;4周龄的小牛、公牛犊和粪便形成的小牛的隐孢子虫感染率高于牛的同类。在靠近人群生活的牲畜和鸡中,需要采取更好的监测和控制措施,以保护脆弱的动物和人群。
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引用次数: 4
First phylogenetic evidence of Trichuris spp. affecting Hamadryas baboon (Papio Hamadryas) in Egypt. 影响埃及Hamadryas狒狒(Papio Hamadryas)的Trichuris sp .的第一个系统发育证据。
Pub Date : 2019-11-01 DOI: 10.21608/EVMSPJ.2019.69620
Amira W. Deweir
Trichuris spp., known as whipworms, are recognized to infect numerous mammalian species including humans and non-human primates. In the present study, forty five fecal samples collected from Hamadryasis baboon at Alexandria Zoo, Egypt were examined microscopically using concentration sedimentation and flotation techniques to detect prevalence of Trichuris spp. Furthermore,the genetic variation of Trichuris spp. recovered from naturally infected Hamadryasis baboon , was analyzed using the ribosomal DNA (ITS) as molecular marker by PCR and sequencing. By combining this dataset with Genbank records for Trichuris isolated from other humans, non-human primates and pigs and phylogenetic analysis, we proved the presence of two distinct Trichuris genotypes that infect baboons, T. trichura and T. suis., which is important for the ongoing treatment of Trichuris that estimated to infect 600 million people worldwide. To our knowledge this is the first study in Egypt genetically characterized Trichuris spp in Hamadryasis baboon, with future prospective to detect genetic characterization of Trichuris in other non-human primates.
鞭虫被认为可以感染许多哺乳动物物种,包括人类和非人类灵长类动物。本研究采用浓缩沉淀法和浮选法对埃及亚历山大动物园采集的45份狒狒粪便样本进行了毛线虫的显微检测,并以其核糖体DNA (ITS)为分子标记,对自然感染的狒狒粪便中毛线虫的遗传变异进行了PCR和测序分析。通过将该数据集与从其他人类、非人类灵长类动物和猪中分离的毛虫的Genbank记录和系统发育分析相结合,我们证明了存在两种不同的毛虫基因型,即感染狒狒的毛虫和猪毛虫。这对正在进行的曲曲菌病治疗非常重要,据估计全世界有6亿人感染曲曲菌。据我们所知,这是埃及首次研究狒狒毛线虫的遗传特征,未来有望在其他非人灵长类动物中检测毛线虫的遗传特征。
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引用次数: 0
Pharmacokinetics and Bioavailability of cefotaxime in broiler chickens 头孢噻肟在肉鸡体内的药动学和生物利用度
Pub Date : 2019-11-01 DOI: 10.21608/evmspj.2019.99307
Mohamed Elhewaty
E-mail: melhewaty@yahoo.com Abstract: After a single intravenous (IV) and intramuscular (IM) injection in broiler chickens, the disposition kinetic profile of cefotaxime was explored. Cefotaxime was administered at 10 mg / kg b.wt dose level for both routes. The serum cefotaxime concentrations estimated at 0.08 h were 47.06 μg / ml after IV injection, which gradually decreased and cefotaxime was identified up to 12 h (0.88 μg / ml). In broiler chickens, the average values of Cltot, Vdss and T0.5 β of cefotaxime were 0.09 L kg -1 h -1 , 0.38 L kg -1 and 2.49 h. The highest serum concentration (Cmax) after IM injection was (22.21 ± 2.03 μg / ml), the maximum serum concentration period (tmax) was (1.16 ± 0.11h) and the half-life of elimination (T0.5 el) was (3.24 ± 0.31h). Bioavailability after IM injection was 84.27%, and in vitro protein binding percent was 28.79 %. A recommended IM dosage for cefotaxime in broiler chickens would be 10 mg / kg b.wt., administered intramuscularly at 12 h intervals, providing a therapeutic serum concentration in broiler chickens exceeding the MIC ≤ 0.5 μg/ml for most sensitive bacterial pathogens in broiler chickens.
摘要:研究了肉仔鸡单次静脉(IV)和肌肉(IM)注射后头孢噻肟的代谢动力学特征。头孢噻肟给药剂量均为10 mg / kg b.wt。静脉注射后0.08 h测定血清头孢噻肟浓度为47.06 μg / ml,随后逐渐降低,至12 h检测到头孢噻肟浓度为0.88 μg / ml。在肉仔鸡中,头孢噻肟的Cltot、Vdss和T0.5 β的平均值分别为0.09 L kg -1、0.38 L kg -1和2.49 h,注射IM后血清最高浓度(Cmax)为(22.21±2.03 μg / ml),最大浓度周期(tmax)为(1.16±0.11h),半衰期(T0.5 el)为(3.24±0.31h)。注射后生物利用度为84.27%,体外蛋白结合率为28.79%。肉仔鸡对头孢噻肟的推荐剂量为每公斤体重10毫克。,每隔12 h肌肉注射一次,对肉鸡中大多数敏感病原菌的治疗血清浓度超过MIC≤0.5 μg/ml。
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引用次数: 0
First finding of antibodies to Encephalitozoon cuniculi in raised chickens in Egypt 在埃及首次在饲养的鸡中发现脑膜炎寄生虫抗体
Pub Date : 2019-11-01 DOI: 10.21608/EVMSPJ.2019.80817
pKaram Imam Ashmawy, Somaia Seif Abuakkadap
20 Abu-Akkada s.s* ;Ashmawy K and EL shanat s. Department of Parasitology, Faculty of Veterinary Medicine, Alexandria University Abis, Alexandria, Egypt Post Code: 21944 *Author of correspondence: Somaia Saif Abu-Akkada Professor of Parasitology Head of Department of Parasitology Acting Vice dean of Postgraduates and researches affairs. Faculty of Veterinary Medicine Alexandria University Egypt E.mail: somaia_abuakkada@yahoo.com Tel. +2 045 2960306 Fax: +2 045 2960450 Abstract:
20 Abu-Akkada s.s*;Ashmawy K和EL shanat s.埃及亚历山大大学阿比斯兽医学院寄生虫学系邮编:21944 *通讯作者:Somaia Saif Abu-Akkada寄生虫学教授,寄生虫学系主任,研究生和研究事务代理副主任。埃及亚历山大大学兽医学院E.mail: somaia_abuakkada@yahoo.com电话:+2 045 2960306传真:+2 045 2960450
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引用次数: 2
期刊
Egyptian Veterinary Medical Society of Parasitology Journal (EVMSPJ)
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