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Three phase purification of milk clotting protease from Wrightia tinctoria fruit and studies on its casein subunit specificity 白桦果实凝乳蛋白酶的三相纯化及其酪蛋白亚基特异性研究
Pub Date : 2021-11-12 DOI: 10.36647/ijanp/01.02.a001
Bassil Yaseen Aljallah, H. P, Sreelakshmi Desai, Uzma Tanzeem, Vasuki Aluru, B. S.
Crude aqueous extract of Wrightia tinctoria fruit was subjected to three phase partitioning (TPP) with three different ammonium sulphate concentrations (40, 60, 80%) and their effect on concentrating milk clotting proteases were examined with 1:1 ratio of crude extract to t-Butanol. Proteases that concentrated in the interphase (IP) fraction of 60 and 80% salt concentration exhibited high milk clotting activity. TPP contributed to reduction in Caseinolytic Activity (CA) and enhancement of Milk Clotting Index (MCI) in these IP fractions compared to the crude enzyme. Casein (whole and kappa) hydrolytic pattern was analyzed by tricine SDS PAGE. Electrophoretic pattern of hydrolysate after 1 hr incubation revealed hydrolysis of κ-CN by CE, IP 60 and IP 80 fractions. Appearance of two low molecular weight bands approximately around 15 kDa indicated the specific affinity and controlled hydrolytic behavior by the proteases. Study provides evidence towards the utility of TPP in concentrating these milk clotting proteases and in improving their MCI. Keywords : Milk clotting • Wrightia tinctoria • Proteases• cheese
以三种不同硫酸铵浓度(40、60、80%)的莱特果粗水提物进行三相分配(TPP),并以粗提物与丁醇的比例为1:1考察其对凝乳蛋白酶浓缩的影响。在盐浓度为60%和80%的间期(IP)部分,蛋白酶表现出较高的凝乳活性。与粗酶相比,TPP有助于降低这些IP组分的酪蛋白溶酶活性(CA)和提高乳凝指数(MCI)。用SDS - PAGE分析酪蛋白(全蛋白和kappa)水解模式。孵育1小时后,水解产物的电泳图谱显示,CE、IP 60和IP 80组分水解了κ-CN。两个低分子量条带的出现大约在15 kDa左右,表明蛋白酶具有特定的亲和力和控制水解行为。研究为TPP在浓缩这些凝乳蛋白酶和改善其MCI方面的应用提供了证据。关键词:凝乳;白曲霉;蛋白酶
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引用次数: 0
A Short Review on The Impact of Junk and Processed Foods on Childhood Obesity 垃圾食品和加工食品对儿童肥胖的影响综述
Pub Date : 2021-11-12 DOI: 10.36647/ijanp/01.01.a001
Jezila Musthafa
A severe public health crisis raises morbidity, mortality and considerable, prolonged social and economic expense is childhood obesity. In the last quarter of the century, the levels of obesity in American children and youth nearly tripled. Nearly 20% of young people are overweight, with obesity rates rising alarmingly in pre-school children. A survey conducted by the Centre for Disease Control and Prevention reported the incidence of obesity in children from 2-5 years and 6-11 years have been doubled from 5-12% and 7%-17% respectively. Childhood obesity puts children as well as adults at an increased risk for developing destitute health including diabetes and cardiovascular complications. To overcome this, preventive measures should emphasize reducing overweight should be implemented. In various contexts, steps to combat childhood obesity must be taken and a multiplicity of interventions implemented and include a broad number of participants. Continual action is needed at multiple levels – individually to impact behavioural change at schools and communities, and sectoral change at the agriculture, food production, training, transport, and urban planning level.
儿童肥胖是一种严重的公共卫生危机,它会提高发病率和死亡率,并造成巨大的、长期的社会和经济损失。在20世纪的最后25年里,美国儿童和青少年的肥胖水平几乎增加了两倍。近20%的年轻人超重,学龄前儿童的肥胖率惊人地上升。疾病控制和预防中心进行的一项调查显示,2-5岁和6-11岁儿童的肥胖发病率分别从5-12%和7%-17%增加了一倍。儿童期肥胖使儿童和成人面临更大的健康不良风险,包括糖尿病和心血管并发症。为了克服这一点,预防措施应该以减少超重为重点。在各种情况下,必须采取防治儿童肥胖的步骤,实施多种干预措施,并包括广泛的参与者。需要在多个层面持续采取行动——个别地影响学校和社区的行为改变,以及农业、粮食生产、培训、交通和城市规划层面的部门变革。
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引用次数: 0
Ayurvedic Nutraceuticals w.s.r.t. seasonal regimen and natural foods : A brief Review 阿育吠陀营养保健品,季节性养生法和天然食品:简要回顾
Pub Date : 2021-11-12 DOI: 10.36647/ijanp/01.02.a003
Dr. Amulya Murthy Aku, Dr. Ashok M Patil
Nutraceutical, a phrase coined from the terms nutrition and pharmaceutical, refers to a food or food product that has health and medical advantages, including illness prevention and treatment. The demand for nutraceuticals has risen in recent years as a result of expanding public knowledge about the importance of good health. Dietary supplements are now widely accessible on the open market. In the United States of America, the nutraceutical sector is worth around 86 billion dollars. In European nations, the figure is somewhat higher. When consumers experience failure with medications, they turn to nutraceuticals or dietary supplements. The illustrious Indian culture has shown that foods may be utilised successfully as medicine to cure and prevent illness. Ayurveda has described the medicinal properties of Ahara (food and nutrition). It is widely accessible and inexpensive, even in the form of homemade cures. The medical food available on the market is far from suitable in terms of price and pharmacological uniformity. Chemically produced meals are likewise detrimental to one's health. Ayurveda contains a plethora of literary sources pertaining to Ahara (food and nutrition) and Vihara (lifestyle). From ancient classical works such as Charak Samhita to more recent classics such as Bhavaprakash, nutrition guidelines have been referenced. Research should be conducted in accordance with sickness and for the purpose of sustaining good health. It is a well known principle in Ayurveda that if you follow a healthy diet there will be no need to take medications. Similarly, the greatest available therapy will be ineffective if the patient does not adhere to dietary limitations. The present paper discusses Ayurvedic nutritional concepts, and feasible and practical Ayurvedic Nutraceutical combinations for various disorders. Keywords : Nutraceuticals, Ayurveda, Health, Wellness, Food.
营养保健品(Nutraceutical)是“nutrition”和“pharmaceutical”两个词的合成词,指的是具有健康和医疗优势的食品或食品,包括预防和治疗疾病。近年来,由于公众对健康重要性的认识不断扩大,对营养保健品的需求有所增加。膳食补充剂现在在公开市场上很容易买到。在美国,营养药品行业价值约860亿美元。在欧洲国家,这一数字略高。当消费者用药失败时,他们会转向营养保健品或膳食补充剂。杰出的印度文化表明,食物可以成功地用作药物来治疗和预防疾病。阿育吠陀已经描述了Ahara(食物和营养)的药用特性。它很容易获得,而且价格低廉,甚至可以自制治疗。市场上现有的医疗食品在价格和药理学上都不合适。化学食品同样对健康有害。阿育吠陀包含了大量与Ahara(食物和营养)和Vihara(生活方式)有关的文献。从《查拉克·萨姆希塔》(Charak Samhita)等古代经典著作到《巴瓦拉卡什》(Bhavaprakash)等近代经典著作,营养指南都被引用。研究应根据疾病情况进行,并以保持良好的健康为目的。这是阿育吠陀的一个众所周知的原则,如果你遵循健康的饮食,就不需要服用药物。同样,如果患者不遵守饮食限制,可用的最佳疗法也将无效。本文讨论了阿育吠陀的营养概念,以及针对各种疾病的可行和实用的阿育吠陀营养组合。关键词:营养药品,阿育吠陀,健康,保健,食品
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引用次数: 0
Eating Habit during Covid-19 Covid-19期间的饮食习惯
Pub Date : 2021-11-12 DOI: 10.36647/ijanp/01.02.a002
Jyotirmayee Sahoo, B. A. Ayushree
A healthy diet is important for good health and nutrition. Optimal nutrition will improve well-being and may mitigate the danger and morbidity associated with coronavirus illness 2019 (COVID-19), caused by the severe acute metabolic process syndrome coronavirus combine of (SARS-CoV-2). This book chapter summarizes nutritional guidelines to support good eating habits during Covid-19. The majority of documents enthused the consumption of fruits, vegetables, and whole grain foods. 31% of the guidelines highlighted the importance of minerals and vitamins such as zinc and vitamin-C, A, and D to keep up a well-functioning immune system. There was no convincing proof that food or food packaging is expounded to the transmission of COVID-19, yet sensible hygiene practices for handling and making preparing foods were counselled. Keywords : SarsCoV2, COVID-19, eating behaviours
健康的饮食对健康和营养很重要。最佳营养将改善健康,并可能降低由严重急性代谢过程综合征冠状病毒组合(SARS-CoV-2)引起的2019年冠状病毒病(COVID-19)相关的危险和发病率。本章总结了在Covid-19期间支持良好饮食习惯的营养指南。大多数文件都热衷于水果、蔬菜和全谷物食品的消费。31%的指南强调了矿物质和维生素(如锌、维生素c、A和D)对保持免疫系统功能良好的重要性。没有令人信服的证据表明食品或食品包装与COVID-19的传播有关,但建议在处理和制备食品时采取合理的卫生措施。关键词:SarsCoV2, COVID-19,饮食行为
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引用次数: 0
Impact of Unbalanced Diet Causing Hormone Imbalance in the Middle-Aged Women 饮食不平衡对中年妇女激素失衡的影响
Pub Date : 2021-11-12 DOI: 10.36647/ijanp/01.01.a003
Nivedha P, R. N.
Hormones perform a crucial role in women's health. Variations in hormone levels (estrogen and progesterone) can negatively affect women's mood, sexual desire, ovulation process, and fertility. A balanced and healthy diet has a major influence on various hormonal processes and facets of our wellbeing. There are multiple factors related to diets, such as food sensitivity leading to allergic reactions, obesity, inflammation from inadequate diet and sedentary lifestyles, sleep habits, digestion, and others. All these factors contribute to hormonal imbalances and associated complications. Diet-derived circulating constituents can perform immediate and indirect actions for receptor activation, signalling, and supplying micronutrients.
荷尔蒙对女性的健康起着至关重要的作用。激素水平(雌激素和黄体酮)的变化会对女性的情绪、性欲、排卵过程和生育能力产生负面影响。平衡和健康的饮食对各种荷尔蒙过程和我们健康的各个方面都有重大影响。与饮食有关的因素有很多,比如导致过敏反应的食物敏感性、肥胖、饮食不足引起的炎症、久坐不动的生活方式、睡眠习惯、消化等等。所有这些因素都会导致荷尔蒙失衡和相关并发症。饮食来源的循环成分可以对受体激活、信号传导和提供微量营养素起直接和间接的作用。
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引用次数: 0
A Study on Anti-Oxidant, Anti-Bacterial, Antifungal, And Anti Platelet Aggregation Potential and Characterization of Ethanolic Extract of Citrus limon (L) Burm. f. Rutacae Peel from Coimbatore 柠檬醇提物抗氧化、抗菌、抗真菌、抗血小板聚集电位及特性研究。6 .产自哥印拜陀的芦果皮
Pub Date : 2021-11-12 DOI: 10.36647/ijanp/01.02.a005
Nivetha M
Therapeutic medicinal plants are the significant constituents of traditional medicine preparations from antiquated occasions. A dominant part of individuals leans toward herbal-based medicines when contrasted with that of regular medication. Safe, effective and inexpensive indigenous remedies have become more popular among the people of both the urban and rural areas of India. Therefore, medicinal plants have become the essential part of the human health care system. Moreover, medicinal plants have attained more attention because of their effectiveness, increased cost of current medicines and cultural preferences. Citrus is presumably place most significant role in commercial and agricultural activities in the world. The peel of the Citrus fruit is a good source of flavanones and numerous polymethoxylated flavones, which are exceptionally uncommon in different plants. The Citrus compounds not just play a significant physiological and natural part but on the other hand, they act as a business intrigue which results in their huge number of utilization in the food and pharmaceutical ventures. Citrus limon (L) burm. f. (lemon) is to be healed of various diseases, and it has various therapeutic, well-being and dietary advantages. Numerous people thought this as a common plant and unaware of its medicinal properties and applications. By this study, we created an aware to grow country based Citrus species and their anti-platelet activity against cardiovascular disease. In this research, pharmacognostical studies in Citrus limon (L) burm. f. has been proved due to the presence of phytoconsituents of the peels. Keywords : Citrus limon, Peel extract, Biological activity, Potential and Characterization, Nutraceuticals.
治疗性药用植物是古代传统药物制剂的重要成分。与常规药物相比,大部分人倾向于草药。安全、有效和廉价的土著疗法在印度城市和农村地区的人民中越来越受欢迎。因此,药用植物已成为人类医疗保健系统的重要组成部分。此外,药用植物由于其有效性、现有药物成本的增加和文化偏好而获得了更多的关注。柑橘大概是世界上商业和农业活动中最重要的角色。柑橘类水果的果皮是黄酮和众多多甲氧基黄酮的良好来源,这些黄酮在不同的植物中非常罕见。柑橘类化合物不仅具有重要的生理和自然作用,而且还具有商业价值,在食品和制药企业中得到了大量利用。柑橘酸橙(L)燃烧。柠檬可以治愈各种疾病,具有多种治疗、保健和饮食方面的优点。许多人认为这是一种常见的植物,不知道它的药用特性和应用。通过本研究,我们建立了一种基于国家的柑橘品种及其抗血小板活性,以预防心血管疾病。本研究对柑桔柠檬(L)的生药学研究。F.由于果皮中存在植物成分而得到了证明。关键词:柠檬皮提取物;生物活性;潜力与表征;
{"title":"A Study on Anti-Oxidant, Anti-Bacterial, Antifungal, And Anti Platelet Aggregation Potential and Characterization of Ethanolic Extract of Citrus limon (L) Burm. f. Rutacae Peel from Coimbatore","authors":"Nivetha M","doi":"10.36647/ijanp/01.02.a005","DOIUrl":"https://doi.org/10.36647/ijanp/01.02.a005","url":null,"abstract":"Therapeutic medicinal plants are the significant constituents of traditional medicine preparations from antiquated occasions. A dominant part of individuals leans toward herbal-based medicines when contrasted with that of regular medication. Safe, effective and inexpensive indigenous remedies have become more popular among the people of both the urban and rural areas of India. Therefore, medicinal plants have become the essential part of the human health care system. Moreover, medicinal plants have attained more attention because of their effectiveness, increased cost of current medicines and cultural preferences. Citrus is presumably place most significant role in commercial and agricultural activities in the world. The peel of the Citrus fruit is a good source of flavanones and numerous polymethoxylated flavones, which are exceptionally uncommon in different plants. The Citrus compounds not just play a significant physiological and natural part but on the other hand, they act as a business intrigue which results in their huge number of utilization in the food and pharmaceutical ventures. Citrus limon (L) burm. f. (lemon) is to be healed of various diseases, and it has various therapeutic, well-being and dietary advantages. Numerous people thought this as a common plant and unaware of its medicinal properties and applications. By this study, we created an aware to grow country based Citrus species and their anti-platelet activity against cardiovascular disease. In this research, pharmacognostical studies in Citrus limon (L) burm. f. has been proved due to the presence of phytoconsituents of the peels. Keywords : Citrus limon, Peel extract, Biological activity, Potential and Characterization, Nutraceuticals.","PeriodicalId":274546,"journal":{"name":"International Journal of Agro Nutrifood Practices","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116096114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Health Benefits of Micronutrients and Stratagies to prevent micronutrient Deficiencies 微量营养素对健康的益处和预防微量营养素缺乏的策略
Pub Date : 2021-11-12 DOI: 10.36647/ijanp/01.01.a002
Prof. Vijaya khader
In metabolism and tissue performance, micronutrients play a key role. Sufficient amount of intake is required to maintain tissue function and metabolic processes. In those people who are extremely depleted and the possibility for complications, the clinical benefits of micronutrient supplements are more advantageous in health improvement. Zinc and vitamin A supplements have contributed to lowered diarrhoea and pneumonia in children in developing countries. In slightly malnourished children, there are some indications that micronutrient supplements have cognitive influence. In several diseases, there is strong proof of the benefit of micronutrient supplements. A well-balanced diet tends to support most of the micronutrients.
在新陈代谢和组织性能方面,微量营养素起着关键作用。足够的摄入量是维持组织功能和代谢过程所必需的。在那些极度缺乏和可能出现并发症的人群中,微量营养素补充剂的临床益处更有利于改善健康。锌和维生素A的补充有助于减少发展中国家儿童的腹泻和肺炎。在轻度营养不良的儿童中,有一些迹象表明微量营养素补充剂对认知有影响。在一些疾病中,有强有力的证据证明微量营养素补充剂的益处。均衡的饮食能提供大部分微量营养素。
{"title":"Health Benefits of Micronutrients and Stratagies to prevent micronutrient Deficiencies","authors":"Prof. Vijaya khader","doi":"10.36647/ijanp/01.01.a002","DOIUrl":"https://doi.org/10.36647/ijanp/01.01.a002","url":null,"abstract":"In metabolism and tissue performance, micronutrients play a key role. Sufficient amount of intake is required to maintain tissue function and metabolic processes. In those people who are extremely depleted and the possibility for complications, the clinical benefits of micronutrient supplements are more advantageous in health improvement. Zinc and vitamin A supplements have contributed to lowered diarrhoea and pneumonia in children in developing countries. In slightly malnourished children, there are some indications that micronutrient supplements have cognitive influence. In several diseases, there is strong proof of the benefit of micronutrient supplements. A well-balanced diet tends to support most of the micronutrients.","PeriodicalId":274546,"journal":{"name":"International Journal of Agro Nutrifood Practices","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132099282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
International Journal of Agro Nutrifood Practices
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