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2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)最新文献

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Tracking single-cells in overcrowded bacterial colonies 在拥挤的菌落中追踪单细胞
A. Balomenos, P. Tsakanikas, E. Manolakos
Cell tracking enables data extraction from timelapse “cell movies” and promotes modeling biological processes at the single-cell level. We introduce a new fully automated computational strategy to track accurately cells across frames in time-lapse movies. Our method is based on a dynamic neighborhoods formation and matching approach, inspired by motion estimation algorithms for video compression. Moreover, it exploits “divide and conquer” opportunities to solve effectively the challenging cells tracking problem in overcrowded bacterial colonies. Using cell movies generated by different labs we demonstrate that the accuracy of the proposed method remains very high (exceeds 97%) even when analyzing large overcrowded microbial colonies.
细胞跟踪可以从延时“细胞电影”中提取数据,并促进单细胞水平的生物过程建模。我们引入了一种新的全自动计算策略来准确地跟踪延时电影帧间的细胞。我们的方法是基于动态邻域形成和匹配方法,受到视频压缩的运动估计算法的启发。此外,它利用“分而治之”的机会,有效地解决了过度拥挤的细菌菌落中具有挑战性的细胞跟踪问题。使用不同实验室生成的细胞片,我们证明即使在分析大型过度拥挤的微生物菌落时,所提出的方法的准确性仍然非常高(超过97%)。
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引用次数: 9
Examining the feasibility of a Microsoft Kinect ™ based game intervention for individuals with anterior cruciate ligament injury risk 研究基于微软Kinect™的游戏干预前交叉韧带损伤风险个体的可行性
Zhiyu Huo, Joseph Griffin, Ryan Babiuch, Aaron D. Gray, Brad W. Willis, Skubic Marjorie, Shining Sun
We describe a feasibility study in which the Microsoft Kinect is used for a game-based exercise to strengthen posterior chain muscles which are often weak in those at high risk of anterior cruciate ligament (ACL) injury. In the game, subjects perform a single posterior chain strengthening exercise. The game uses a side-scrolling video display driven by a hip abduction exercise while a player lies down on the floor. Leg lifts beyond a predetermined angle trigger the jumping action of an animated tiger. We describe the scene and game control, which uses depth images from the Kinect. Although Kinect-based skeletal data are used for many games, the skeletal model does not yield good estimates for positions on the floor. Our proposed system uses multiple leg angle estimators for different angle regions to recognize the player lying down and capture the angle between two legs. We conducted an experiment that validates our system with marker-based Vicon ground truth data. We also present results of an end-to-end test using the game, showing feasibility.
我们描述了一项可行性研究,在该研究中,微软Kinect用于基于游戏的锻炼,以加强前交叉韧带(ACL)损伤高风险人群中经常薄弱的后链肌肉。在游戏中,受试者进行单一的后链强化练习。当玩家躺在地板上时,游戏使用了一个侧面滚动的视频显示,由髋关节外展运动驱动。腿抬起超过预定角度触发动画老虎的跳跃动作。我们使用Kinect的深度图像来描述场景和游戏控制。尽管基于kinect的骨骼数据用于许多游戏,但骨骼模型并不能很好地估计地板上的位置。我们提出的系统使用不同角度区域的多个腿部角度估计器来识别躺着的球员并捕获两条腿之间的角度。我们进行了一个实验,用基于标记的Vicon地面真实数据验证了我们的系统。我们还介绍了使用该游戏进行端到端测试的结果,显示了可行性。
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引用次数: 4
Emergence of critical dynamics in large-scale in vitro cortical networks 大规模体外皮层网络中临界动力学的出现
P. Massobrio, Valentina Pasquale, S. Martinoia
In vitro neuronal networks coupled to Micro-Electrode Arrays (MEAs) represent a valid experimental framework to study neuronal dynamics. This preparation is free of chemical or physical constraints and allows neurons to self-organize during development, creating networks that exhibit complex spatio-temporal patterns of activity. Starting from this experimental evidence, here we address the question whether a particular network architecture can drive the network dynamics towards a sub-, super-, or critical state.
体外神经网络耦合微电极阵列(MEAs)是研究神经元动力学的有效实验框架。这种制备不受化学或物理限制,允许神经元在发育过程中自我组织,创造出具有复杂时空活动模式的网络。从这个实验证据开始,我们在这里解决了一个问题,即特定的网络架构是否可以将网络动态推向亚、超或临界状态。
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引用次数: 3
A miniaturized monitoring system for electrochemical biosensing using Shewanella oneidensis in environmental applications 基于希瓦氏菌的微型电化学生物传感监测系统在环境中的应用
A. Y. Zhou, Tom J. Zajdel, Michaela A. Teravest, M. Maharbiz
We present a miniaturized, free-floating monitoring system which makes use of electron transfer in Shewanella oneidensis sequestered behind a permeable membrane while maintaining diffusive contact with the environment, allowing for sensing environmental conditions. The system makes use of a commercial off-the-shelf (COTS) integrated circuit (IC) which sets a potential between a working electrode and a Ag/AgCl reference electrode while recording the resulting current from the electroactive cells. We successfully sensed both pyruvate and the environmental presence of E. coli via changes in the currents sensed. This work will enable the development of mobile aquatic sensing systems which make use of bacterial electron transfer as a transduction method. Further miniaturization of the recording mote, electrodes, packaging, and system is discussed.
我们提出了一种小型化、自由漂浮的监测系统,该系统利用隔离在可渗透膜后面的希瓦氏菌中的电子转移,同时保持与环境的扩散接触,从而可以感知环境条件。该系统利用商用现货(COTS)集成电路(IC),在工作电极和Ag/AgCl参考电极之间设置电势,同时记录来自电活性电池的产生电流。通过感应电流的变化,我们成功地感应到了丙酮酸盐和大肠杆菌在环境中的存在。这项工作将使利用细菌电子转移作为转导方法的移动水生传感系统的发展成为可能。讨论了记录模、电极、封装和系统的进一步小型化。
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引用次数: 7
Wearable real-time ecg monitoring with emergency alert system for scuba diving 带紧急警报系统的可穿戴式实时心电监护
Tobias Cibis, B. Groh, Heike Gatermann, Heike Leutheuser, B. Eskofier
Medical diagnosis is the first level for recognition and treatment of diseases. To realize fast diagnosis, we propose a concept of a basic framework for the underwater monitoring of a diver's ECG signal, including an alert system that warns the diver of predefined medical emergency situations. The framework contains QRS detection, heart rate calculation and an alert system. After performing a predefined study protocol, the algorithm's accuracy was evaluated with 10 subjects in a dry environment and with 5 subjects in an underwater environment. The results showed that, in 3 out of 5 dives as well as in dry environment, data transmission remained stable. In these cases, the subjects were able to trigger the alert system. The evaluated data showed a clear ECG signal with a QRS detection accuracy of 90 %. Thus, the proposed framework has the potential to detect and to warn of health risks. Further developments of this sample concept can imply an extension for monitoring different biomedical parameters.
医学诊断是认识和治疗疾病的第一级。为了实现快速诊断,我们提出了一种水下监测潜水员心电信号的基本框架概念,其中包括一个警报系统,该系统可以向潜水员警告预定的医疗紧急情况。该框架包含QRS检测、心率计算和警报系统。在执行预定义的研究方案后,对干燥环境中的10名受试者和水下环境中的5名受试者进行了算法的准确性评估。结果表明,在5次潜水中有3次以及干燥环境下,数据传输保持稳定。在这些情况下,受试者能够触发警报系统。评估数据显示心电信号清晰,QRS检测准确率达90%。因此,拟议的框架具有发现和警告健康风险的潜力。该示例概念的进一步发展可能意味着监测不同生物医学参数的扩展。
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引用次数: 15
A proposal for improvement of genotyping performance for ethnically homogeneous population using DNA microarray 利用DNA微阵列技术提高种族同质群体基因分型性能的建议
Arika Fukushima, T. Paul, Ryusei Shingaki, Takashi Koiso, Shinya Umeno, Ken Ueno
DNA microarray is used to determine the genotypes of several hundred thousand to several million SNPs (Single Nucleotide Polymorphisms) on multiple samples at a time. In the conventional method of genotyping using DNA microarray, it is assumed that each SNP has three types of genotypes: two homozygous and one heterozygous genotypes. However, in an ethnically homogeneous population, there are cases when all the samples of a SNP belong to one homozygous genotype, and there are some other cases, especially in the SNPs of low MAFs (Minor Allele Frequencies), each sample belongs to either of the two genotypes: one homozygous and one heterozygous genotypes. In those cases, the conventional method of genotyping may fail to properly determine the genotypes of the samples. In this paper, we propose a new genotyping method, which can be used as a post-processing technique of the conventional genotyping method, for re-judgment of the SNPs having one or two types of genotypes. The proposed method takes fluctuations of the fluorescence intensities of the signals of DNA microarray into account, assigns genotypes to samples from those genotype patterns that may occur under natural mating conditions and applies different genotype judgment methods depending on the number of genotype clusters of a SNP. We evaluate our proposed method using the data of 1000 Genome Project and have found that our proposed method is able to improve the genotyping performance of the conventional method.
DNA微阵列用于同时确定多个样品上数十万至数百万个snp(单核苷酸多态性)的基因型。在传统的DNA微阵列基因分型方法中,假设每个SNP具有三种基因型:两种纯合基因型和一种杂合基因型。然而,在种族同质群体中,存在一个SNP的所有样本都属于一个纯合基因型的情况,也有一些其他情况,特别是在低MAFs (Minor等位基因频率)的SNP中,每个样本都属于两个基因型中的一个:一个纯合基因型和一个杂合基因型。在这些情况下,传统的基因分型方法可能无法正确地确定样品的基因型。本文提出了一种新的基因分型方法,该方法可以作为传统基因分型方法的后处理技术,用于对具有一种或两种基因型的snp进行重新判断。该方法考虑到DNA微阵列信号的荧光强度波动,对自然交配条件下可能出现的基因型模式的样本进行基因型分配,并根据SNP基因型簇的数量采用不同的基因型判断方法。我们使用1000基因组计划的数据对所提出的方法进行了评估,发现所提出的方法能够提高传统方法的基因分型性能。
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引用次数: 1
Modeling of blood flow through sutured micro-vascular anastomoses 微血管吻合口缝合血流模型
G. Karanasiou, Dimitrios A. Gatsios, M. Lykissas, K. Stefanou, G. Rigas, I. Lagaris, Ioannis P. Kostas-Agnantis, I. Gkiatas, A. Beris, D. Fotiadis
Microanastomosis is a surgical procedure used to reconnect two blood vessels using sutures. The optimal microanastomosis may be predicted by assessing the factors that influence this invasive procedure. Blood flow and hemodynamics following microanastomosis are important factors for the successful longevity of this operation. How is the blood flow affected by the presence of sutures? Computational Fluid Dynamics (CFD) is a powerful tool that permits the estimation of specific quantities, such as fluid stresses, that are hardly measurable in vivo. In this study, we propose a methodology which evaluates the alterations in the hemodynamic status due to microanastomosis. A CFD model of a reconstructed artery has been developed, based on anatomical information provided by intravascular ultrasound and angiography, and was used to simulate blood flow after microanastomosis. The 3D reconstructed arterial segments are modeled as non-compliant 1.24 - 1.47 mm diameter ducts, with approximately 0.1 mm arterial thickness. The blood flow is considered laminar and the no-slip condition is imposed on the boundary wall, which is assumed to be rigid. In analyzing the results, the distribution of the wall shear stress (WSS) is presented in the region of interest, near the sutures. The results indicate that high values of WSS appear in the vicinity of sutures. Such regions may promote thrombus formation and subsequently anastomotic failure, therefore their meticulous study is of high importance.
微吻合是一种用缝合线重新连接两根血管的外科手术。通过评估影响这种侵入性手术的因素,可以预测最佳微吻合。微吻合术后的血流和血流动力学是手术成功的重要因素。缝合线对血流有何影响?计算流体动力学(CFD)是一种强大的工具,它允许估计特定的数量,如流体应力,这些在体内很难测量。在这项研究中,我们提出了一种评估微吻合引起的血流动力学状态改变的方法。基于血管内超声和血管造影提供的解剖学信息,建立了重建动脉的CFD模型,并用于模拟微吻合后的血流。三维重建的动脉段被建模为直径1.24 - 1.47 mm的非弯曲导管,动脉厚度约为0.1 mm。血流假定为层流,边界壁假定为刚性,并施加无滑移条件。在分析结果时,壁剪应力(WSS)的分布呈现在感兴趣的区域,靠近缝合线。结果表明,高WSS值出现在缝合线附近。这些区域可能促进血栓形成和随后的吻合口衰竭,因此对它们的细致研究是非常重要的。
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引用次数: 7
Producing 3D neuronal networks in hydrogels for living bionic device interfaces 在水凝胶中制造三维神经网络,用于活体仿生设备接口
Ulises A. Aregueta-Robles, K. Lim, P. Martens, N. Lovell, L. Poole-Warren, R. Green
Hydrogels hold significant promise for supporting cell based therapies in the field of bioelectrodes. It has been proposed that tissue engineering principles can be used to improve the integration of neural interfacing electrodes. Degradable hydrogels based on poly (vinyl alcohol) functionalised with tyramine (PVA-Tyr) have been shown to support covalent incorporation of non-modified tyrosine rich proteins within synthetic hydrogels. PVA-Tyr crosslinked with such proteins, were explored as a scaffold for supporting development of neural tissue in a three dimensional (3D) environment. In this study a model neural cell line (PC12) and glial accessory cell line, Schwann cell (SC) were encapsulated in PVA-Tyr crosslinked with gelatin and sericin. Specifically, this study aimed to examine the growth and function of SC and PC12 co-cultures when translated from a two dimensional (2D) environment to a 3D environment. PC12 differentiation was successfully promoted in both 2D and 3D at 25 days post-culture. SC encapsulated as a single cell line and in co-culture were able to produce both laminin and collagen-IV which are required to support neuronal development. Neurite outgrowth in the 3D environment was confirmed by immunocytochemical staining. PVA-Tyr/sericin/gelatin hydrogel showed mechanical properties similar to nerve tissue elastic modulus. It is suggested that the mechanical properties of the PVA-Tyr hydrogels with native protein components are providing with a compliant substrate that can be used to support the survival and differentiation of neural networks.
水凝胶在支持生物电极领域的细胞疗法方面具有重要的前景。有人提出,组织工程原理可用于改善神经接口电极的集成。以酪胺(PVA-Tyr)功能化的聚乙烯醇(乙烯醇)为基础的可降解水凝胶已被证明支持非修饰的富含酪氨酸的蛋白质在合成水凝胶中的共价结合。与这些蛋白交联的PVA-Tyr被探索作为支持三维(3D)环境中神经组织发育的支架。本实验将模型神经细胞系PC12和神经胶质副细胞系雪旺细胞(SC)包裹在明胶和丝胶交联的PVA-Tyr中。具体来说,本研究旨在研究SC和PC12共培养物从二维(2D)环境转化为三维环境时的生长和功能。在培养后25天,PC12在2D和3D中都成功地促进了分化。SC被封装为单细胞系,并在共培养中能够产生层粘连蛋白和胶原- iv,这是支持神经元发育所必需的。免疫细胞化学染色证实神经突在三维环境下生长。PVA-Tyr/丝胶/明胶水凝胶力学性能与神经组织弹性模量相似。这表明,含有天然蛋白质成分的PVA-Tyr水凝胶的力学性能为支持神经网络的存活和分化提供了一种柔顺的底物。
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引用次数: 12
Breast tumor detection using UWB circular-SAR tomographic microwave imaging 超宽带环形sar层析成像微波成像检测乳腺肿瘤
D. Oloumi, P. Boulanger, A. Kordzadeh, K. Rambabu
This paper describes the possibility of detecting tumors in human breast using ultra-wideband (UWB) circular synthetic aperture radar (CSAR). CSAR is a subset of SAR which is a radar imaging technique using a circular data acquisition pattern. Tomographic image reconstruction is done using a time domain global back projection technique adapted to CSAR. Experiments are conducted on a breast phantoms made of pork fat emulating normal and cancerous conditions. Preliminary experimental results show that microwave imaging of a breast phantom using UWB-CSAR is a simple and low-cost method, efficiently capable of detecting the presence of tumors.
本文介绍了利用超宽带(UWB)圆形合成孔径雷达(CSAR)检测乳腺肿瘤的可能性。CSAR是SAR的一个子集,SAR是一种使用圆形数据采集模式的雷达成像技术。层析成像的重建采用了一种适用于CSAR的时域全局反投影技术。实验是在一个由猪肉脂肪制成的乳房模型上进行的,模拟正常和癌变的情况。初步实验结果表明,利用超宽带- csar技术对乳房假体进行微波成像是一种简单、低成本的方法,能够有效地检测肿瘤的存在。
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引用次数: 16
r-principal subspace for driver cognitive state classification 驾驶员认知状态分类的r-主子空间
H. Almahasneh, N. Kamel, N. Walter, A. Malik
Using EEG signals, a novel technique for driver cognitive state assessment is presented, analyzed and experimentally verified. The proposed technique depends on the singular value decomposition (SVD) in finding the distributed energy of the EEG data matrix A in the direction of the r-principal subspace. This distribution is unique and sensitive to the changes in the cognitive state of the driver due to external stimuli, so it is used as a set of features for classification. The proposed technique is tested with 42 subjects using 128 EEG channels and the results show significant improvements in terms of accuracy, specificity, sensitivity, and false detection in comparison to other recently proposed techniques.
提出了一种基于脑电信号的驾驶员认知状态评估方法,并对其进行了分析和实验验证。该方法依靠奇异值分解(SVD)来寻找脑电数据矩阵A在r主子空间方向上的分布能量。这种分布是独特的,对驾驶员在外界刺激下认知状态的变化非常敏感,因此可以作为一组特征进行分类。采用128个EEG通道对42名受试者进行了测试,结果表明,与其他最近提出的技术相比,该技术在准确性、特异性、灵敏度和误检方面有显著提高。
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引用次数: 0
期刊
2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
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