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Abstracts book of Pulse of Asia 2023 《亚洲脉搏2023》摘要
Q1 PERIPHERAL VASCULAR DISEASE Pub Date : 2023-01-01 DOI: 10.1159/000534378
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引用次数: 0
The Pulse of Asia 2022 Tokyo 亚洲脉搏2022东京
IF 2.2 Q1 PERIPHERAL VASCULAR DISEASE Pub Date : 2022-06-27 DOI: 10.1159/000525603
None
Pulse 2022;10:1–34
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引用次数: 0
Simultaneous Detection and Differentiation of Chikungunya Virus and Dengue Virus in Blood at Clinical Presentation by Real Time RT-PCR 基孔肯雅病毒和登革热病毒临床表现的实时RT-PCR同时检测和鉴别
IF 2.2 Q1 PERIPHERAL VASCULAR DISEASE Pub Date : 2018-10-19 DOI: 10.3329/pulse.v10i1.38605
MM Rahman, R Rahim, AH Rasel, AS Murad
Background: Chikungunya viral infection in Bangladesh has marked 2017 summer with unprecedented magnitude for the first time. Chikungunya virus (CHIKV) and Dengue virus (DENV) are arboviruses that share the same Aedes mosquito vectors and thus overlap in their endemic areas.These two viruses cause similar clinical presentations, especially in the initial stages of infection. Because the outcomes and management strategies for these two viruses are different, early and accurate diagnosis is imperative. Early diagnosis is also important for surveillance and outbreak control. Objective: As virus isolation is not undergoing in the country and antigen based commercial detection assay is not available for CHIKV we used one step real time reverse transcriptase polymerase chain reaction (RT-PCR) method to detect and discriminate CHIKV and DENV in blood during suspicious clinical symptoms. Results: By this RT-PCR method we have detected 603 cases of CHIKV and 233 cases of DENV and thus facilitated rapid diagnosis and clinical management in the recent CHIKV outbreaks in the country. Conclusion: This is the first report about molecular detection and differentiation of CHIKV and DENV at time of clinical presentation and further show evidence of simultaneous outbreaks of both the viral infections in the country. Pulse Vol.10 January-December 2017 p.6-11
背景:2017年夏季,孟加拉国的基孔肯雅病毒感染首次以前所未有的规模出现。基孔肯雅病毒(CHIKV)和登革热病毒(DENV)是虫媒病毒,它们具有相同的伊蚊载体,因此在其流行地区重叠。这两种病毒引起相似的临床表现,特别是在感染的最初阶段。由于这两种病毒的结果和管理策略不同,早期和准确的诊断是必不可少的。早期诊断对监测和疫情控制也很重要。目的:由于国内尚未进行病毒分离,且没有基于抗原的商业检测方法,我们采用一步实时逆转录酶聚合酶链反应(RT-PCR)方法在可疑临床症状时检测和区分血液中的CHIKV和DENV。结果:本方法共检出603例CHIKV病例和233例DENV病例,为我国近期发生的CHIKV疫情的快速诊断和临床管理提供了便利。结论:这是第一份关于在临床表现时对CHIKV和DENV进行分子检测和区分的报告,并进一步显示了该国同时暴发这两种病毒感染的证据。《脉冲》Vol.10 2017年1 - 12月p.6-11
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引用次数: 1
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Pulse
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