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Determination of Genetic Diversity of Sinapis arvensis L. (Wild Mustard) by Microsatellite Markers 通过微卫星标记确定野芥菜(Sinapis arvensis L.)的遗传多样性
Pub Date : 2024-01-22 DOI: 10.16955/bitkorb.1378621
Okan Güzel, Emine Kaya Altop
Herbicides are heavily used to manage Sinapis arvensis, mostly a self-fertilizing weed that poses issues in wheat fields. Cross-fertilization may lead to genetic differentiation of this species. The aim of this study was to identify the genetic diversity of individuals collected from wheat fields across various regions in Turkey. The degree of genetic variation in wild mustard populations was evaluated using 5 simple sequence repeat (SSR) markers in samples taken from 30 different locations in Türkiye. Populations were analyzed using UPGMA (unweighted pair group method with arithmetic mean) and principal component analysis (PCA). The mean genetic diversity (GD) and polymorphism information content (PIC) values were 0.752 and 0.844, respectively. Results showed high genetic variability among individual genotypes within geographic locations. The populations were categorized into two major groups, as indicated by the UPGMA dendrogram generated using Average Linkage. There was no apparent geographic isolation among the genotypes of the studied wild mustard, which displayed a high degree of variability. It is recognized that the primary source of this condition is the adaptability of wild mustard seeds dispersed through various methods across different locations. Despite being a predominantly self-pollinating species, they may also employ some cross-pollination mechanisms. In conclusion, SSR markers prove useful in determining genetic diversity in outcrossing species, especially in cases where no prior genotypic information is available. The study suggests that genetic diversity is maintained in wild mustard populations even with rotational farming practices and the use of herbicides.
除草剂被大量用于管理禾本科杂草(Sinapis arvensis),它主要是一种自肥杂草,在小麦田中造成问题。杂交受精可能会导致该物种的遗传分化。本研究旨在确定从土耳其不同地区麦田采集的个体的遗传多样性。研究人员使用 5 个简单序列重复(SSR)标记评估了从土耳其 30 个不同地点采集的样本中野生芥菜种群的遗传变异程度。使用 UPGMA(算术平均非加权配对组法)和主成分分析法(PCA)对种群进行了分析。遗传多样性(GD)和多态性信息含量(PIC)的平均值分别为 0.752 和 0.844。结果表明,在不同地理位置上,单个基因型之间的遗传变异性很高。使用平均关联法生成的 UPGMA 树枝图显示,种群被分为两大类。所研究的野生芥菜基因型之间没有明显的地理隔离,显示出高度的变异性。人们认识到,造成这种情况的主要原因是野生芥菜种子通过各种方法散播到不同地点后的适应性。尽管它们主要是自花授粉物种,但也可能采用一些异花授粉机制。总之,SSR 标记证明有助于确定外交物种的遗传多样性,尤其是在没有先前基因型信息的情况下。研究表明,即使采用轮作耕作法和使用除草剂,野生芥菜种群的遗传多样性仍能保持。
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引用次数: 0
Fumigant and Contact Toxicity of Ruta Chalepensis L. (Rutaceae) Essential Oil Against Five Coleopteran Stored Product Pests and Its Effects On Cholinesterases Ruta Chalepensis L.(芸香科)精油对五种鞘翅目储藏产品害虫的熏蒸和接触毒性及其对胆碱酯酶的影响
Pub Date : 2023-12-04 DOI: 10.16955/bitkorb.1374633
Y. Yücel, Ömer Karakoç, Hüseyin Servi, S. Gücel, Ayşe Nalbantsoy, K. Polatoğlu
The essential oil composition of aerial parts of Ruta chalepensis L. was analyzed with GC-MS. Seventy-nine compounds were detected representing 85.93 ± 1.08% (n = 3) of the essential oil. The major components of the essential oil were 2-undecanone 21.52±0.21%, 2-nonanone 18.31 ± 0.27%, and 2-nonyl acetate 13.22%. The highest insecticidal contact toxicity of the oil was observed against Rhyzopertha dominica F. with 0.018 μL/insect LD50 and 0.039 μL/insect LD90 after 24h. Essential oil also produced considerably low 0.50 and 0.59 μL/insect LD50 values after 24h against Sitophilus oryzae L. and Sitophilus granarius L. respectively. The lowest contact toxicity was observed against Tribolium castaneum Herbst. and Tribolium confusum Jacquelin du Val. 0.138 and 0.078 μL/insect LD50 after 24h respectively. The highest fumigant toxicity was observed against S. granarius for the application concentration of 10 μL, 10% oil/acetone (v:v) in a 10 mL chamber which afforded 100.00 ± 0.00 % mortality after 48h. The essential oil also produced high fumigant toxicity against S. oryzae, T. castaneum, and R. dominica which were 95.47 ± 3.41%, 93.30±5.54%, and 85.47 ± 3.41% mortality at 20 μL application concentration of the oil solution after 48h. The R. chalepensis essential oil produced low acetylcholinesterase enzyme 5.29 ± 1.20% (n=3) inhibition and mediocre butyrylcholinesterase enzyme inhibition 42.6 ± 0.71% (n=3). According to the insecticidal activity assays performed, R. chalepensis essential oil seems to be a promising source that could yield natural compounds that could be employed in stored product pest management.
采用 GC-MS 分析了 Ruta chalepensis L. 气生部分的精油成分。检测到 79 种化合物,占精油的 85.93 ± 1.08% (n = 3)。精油的主要成分为 2-十一酮(21.52±0.21%)、2-壬酮(18.31±0.27%)和 2-壬基乙酸酯(13.22%)。精油对 Rhyzopertha dominica F. 的杀虫接触毒性最高,24 小时后的半数致死剂量为 0.018 μL/insect LD50,半数致死剂量为 0.039 μL/insect LD90。24 小时后,精油对稻田蝇(Sitophilus oryzae L.)和谷斑皮蠹(Sitophilus granarius L.)的半数致死剂量分别为 0.50 μL 和 0.59 μL,也相当低。对 Tribolium castaneum Herbst.和 Tribolium confusum Jacquelin du Val.的接触毒性最低,分别为 0.138 和 0.078。24 小时后,每只昆虫的半数致死剂量分别为 0.138 和 0.078 微升。在 10 mL 的熏蒸室中施用浓度为 10 μL、10% 的精油/丙酮(v:v)的熏蒸剂对 S. granarius 的毒性最高,48 小时后死亡率为 100.00 ± 0.00%。精油对 S. oryzae、T. castaneum 和 R. dominica 的熏蒸毒性也很高,在 20 μL 的精油溶液浓度下,48 小时后的死亡率分别为 95.47 ± 3.41%、93.30±5.54% 和 85.47 ± 3.41%。R.chalepensis精油对乙酰胆碱酯酶的抑制率较低,为5.29±1.20%(n=3),对丁酰胆碱酯酶的抑制率一般,为42.6±0.71%(n=3)。根据所进行的杀虫活性测定,R. chalepensis 精油似乎是一种很有前景的来源,可以产生天然化合物,用于储藏产品的害虫管理。
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引用次数: 0
Determination of harmful and beneficial predator insect species and the distribution and density of Eurygaster integriceps Puton (Hemiptera: Scutelleridae) in wheat-cultivated areas of Siirt province 锡尔特省小麦栽培区益害掠食性昆虫种类测定及半翅目:圆翅大翅蛾的分布和密度
Pub Date : 2023-06-30 DOI: 10.16955/bitkorb.1095875
H. Dilmen, C. Kaplan, M. S. Özgökçe, Mustafa Cemal Çi̇ftçi̇, Meryem ÖZER DİLMEN, F. Pala, M. Kaplan
Wheat is nowadays the most important source of food on earth. Many pest species have been identified that affect wheat yield and quality negatively. This study was conducted to identify harmful and beneficial insect (predator) species and of the distribution of Sunn pest in wheat cultivation areas in the districts (Siirt province Merkez, Kurtalan, Eruh, Tillo, Şirvan, Pervari, and Baykan) of Siirt Province, Türkiye in 2018 and 2019. Samplings were taken periodically for 1-2 weeks from April to July. The sweep nets, frame, pitfall trap, and visual control methods were used for sampling. At the end of the study, 42 species belonging to 6 orders and 25 families were determined. The distributions of the species were recorded as 27 species belonging to 12 families in Coleoptera, 10 species belonging to 7 families in Hemiptera, 2 species belonging to 2 families in Neuroptera, and 1 species belonging to each family in Hymenoptera, Orthoptera and Diptera. The most common and abundant species: the main pest Eurygaster integriceps Puton, 1881 (Hemiptera: Scutelleridae), the secondary pests Aelia acuminata (Linnaeus, 1758), Dolycoris baccarum (Linnaeus, 1758) (Hemiptera: Pentatomidae), Cephus pygmaeus (Linnaeus, 1767) (Hymenoptera: Cephidae) Gryllus bimaculatus De Geer, 1773, (Orthoptera: Gryllidae) were determined. In the study, a total of 16 species were identified, which is the first record for the local fauna of Siirt province. The highest average density of E. integriceps (2 adult + nymph / m2) was found in the Garzan and Gozpinari locations of the Kurtalan district. Besides, among the beneficial species, general predators Chrysoperla carnea (Stephens, 1836) and Coccinella septempunctata (Linnaeus, 1758) are remark as the most common species
小麦现在是地球上最重要的食物来源。许多有害生物对小麦产量和品质有负面影响。本研究于2018年和2019年在锡伊特省(Merkez、Kurtalan、Eruh、Tillo、Şirvan、Pervari和Baykan)和土耳其(rkiye)地区的小麦种植区进行了有害和有益昆虫(捕食者)种类鉴定和太阳害虫分布调查。4 ~ 7月定期取样1 ~ 2周。采用扫网法、框架法、陷阱法和目视控制法进行取样。研究结束后,共鉴定出6目25科42种。其中鞘翅目12科27种,半翅目7科10种,神经翅目2科2种,膜翅目、直翅目和双翅目各科1种。其中最常见和最丰富的害虫有:主要害虫全翅大翅蛾,1881(半翅目:小圆翅蛾科),次要害虫尖翅大翅蛾(Linnaeus, 1758),小圆翅蛾(Linnaeus, 1758),小圆翅蛾(Linnaeus, 1767)(膜翅目:小圆翅蛾科),大圆翅蛾(Linnaeus, 1767)(直翅目:小圆翅蛾科)。本研究共鉴定出16种,首次记录了锡尔特省当地动物群。库尔塔兰地区加尔赞和戈兹皮纳里的平均密度最高,为2只成虫+若虫/ m2。此外,在有益种中,最常见的是一般捕食者Chrysoperla carnea (Stephens, 1836)和七星瓢虫Coccinella七星瓢虫(Linnaeus, 1758)
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引用次数: 0
Anastomosis grouping and phylogenetic analysis of Rhizoctonia isolates on wheat in Türkiye 小麦根丝核菌分离株吻合群与系统发育分析
Pub Date : 2023-06-30 DOI: 10.16955/bitkorb.1263982
F. Ünal, Sara Dolar
This study aims to determine the species and evaluate the genetic diversity of the pathogenic and nonpathogenic Rhizoctonia spp. and anastomosis groups (AG) from wheat plants and rhizosphere soils in Turkey. Rhizoctonia species were isolated from plants and rhizosphere soils in wheat fields in 5 provinces in the Central Anatolian Region of Türkiye. As a result of the isolations, a total of 88 multinucleate (MN) and binucleate (BN) Rhizoctonia isolates were obtained. Identifications of the isolates were determined by rDNA-ITS sequence analyses. The identified isolates belonged to MN Waitea circinata var. zeae, W. circinata var. oryzae, W. circinata var. circinata, MN Rhizoctonia solani AG 2-1, AG 2-2, AG 3, AG 4-HGII, AG 4-HGIII, AG 5, AG 8, AG 11 and BN AG A, AG DI, AG E, AG G, AG H, AG I, AG I-like and AG K. The most isolated group was W. circinata var. circinata. In the pathogenicity studies, the most virulent group was determined as R. solani AG 4. Among the binucleate isolates, groups other than R. cerealis AG DI were not found to be pathogenic. Neighbor-joining phylogenetic trees of isolates were constructed from rDNA-ITS sequences. As a result of this study, the regional distribution of MN and BN Rhizoctonia AG isolates in important wheat production areas in the Central Anatolia Region, Türkiye was determined. In addition, this study is the first comprehensive study in which the genetic diversity of Rhizoctonia AGs isolates obtained from wheat and rhizosphere soils in the region was evaluated with a molecular approach.
本研究旨在确定土耳其小麦植株和根际土壤中致病性和非致病性根核菌属和吻合群(AG)的种类,并评价其遗传多样性。对基耶省中部安那托利亚地区5个省的小麦植株和根际土壤中分离到的根丝核菌进行了研究。结果表明,共分离得到88株多核(MN)和双核(BN)根核菌。采用rDNA-ITS序列分析鉴定分离菌株。鉴定的分离菌株分别为玉米白丝胞菌、玉米白丝胞菌、玉米白丝胞菌、玉米白丝胞菌AG 2-1、AG 2-2、AG 3、AG 4-HGII、AG 4-HGIII、AG 5、AG 8、AG 11和BN AG A、AG DI、AG E、AG G、AG H、AG I、AG I样和AG k,分离菌株最多的是玉米白丝胞菌。在致病性研究中,确定了最强毒群为R. solani ag4。在双核分离株中,除谷鼠AG - DI外,其余各组均未发现致病性。利用rDNA-ITS序列构建了相邻连接的系统发育树。本研究确定了中安纳托利亚地区 rkiye重要小麦产区MN和BN根核菌AG分离株的区域分布。此外,本研究首次采用分子方法对该地区小麦和根际土壤中分离的根丝核菌AGs进行了遗传多样性评价。
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引用次数: 0
TURUNÇGİL BAHÇESİNDE FARKLI RENK TUZAKLARINDAKİ YAPRAKBİTİ AKTİVİTESİ
Pub Date : 2023-03-16 DOI: 10.16955/bitkorb.1253486
M. Karacaoğlu, Işıl Özdemi̇r, Mustafa Özdemir, Serdar Satar
Aphids feeds on sap from phloem and xylem vessels, this can cause honeydew secretion. This injury is induced by apterous aphid groups, however alate groups can cause other economically important problems such as virus transmission. Apterous population of aphids are controlled by cultural, biological or chemical control methods. Flight activity of alate populations are much difficult to control because a short time, like a minute or half an hour, is enough for single individuals to transmit viral diseases. The first and most critical step in monitoring alate aphid population is to use the right integrated pest management strategies (IPM). The present study was carried out in Department of Plant Protection Experimental Station of Çukurova University between October 2009 to October 2010 in order to understand the dispersal time of aphid and right sampling methods of alate individuals. Approximately 400 citrus plants were planted spaced of 5.0 m × 3.0 m apart in the orchard in September 2002. Painted water tray (18 X 32 cm) filled with tap water and small amount of liquid soap were placed between rows. Each trap was placed on platform 120 cm from ground. White, red, blue, yellow and green colored traps were used as to compare the color preference of alate aphids. All the individuals caught were identified under the microscope after mounting. After identification their cluster analyzes according to color choice and similarity index were assessed. This research was presented as a poster in the 10th International Symposium on Aphids (4‐8 September 2017, Cappadocia, Türkiye)
蚜虫以韧皮部和木质部血管的汁液为食,这可以引起蜜汁的分泌。这种伤害是由无尾蚜群引起的,然而无尾蚜群可以引起其他经济上重要的问题,如病毒传播。蚜虫的无翅种群可通过培养、生物或化学防治方法加以控制。鸟类的飞行活动很难控制,因为一分钟或半小时这样的短时间就足以让单个个体传播病毒性疾病。监测蚜虫种群的第一步也是最关键的一步是使用正确的害虫综合管理策略(IPM)。本研究于2009年10月至2010年10月在Çukurova大学植物保护实验站进行,目的是了解蚜虫的传播时间和正确的采样方法。2002年9月,在果园内以5.0 m × 3.0 m的间距种植柑橘植株约400株。在两排之间放置装有自来水和少量液体肥皂的油漆水盘(18 X 32厘米)。每个陷阱放置在距离地面120厘米的平台上。采用白、红、蓝、黄、绿四色诱捕器比较了褐蚜对颜色的偏好。所有捕获的个体在装上后在显微镜下进行鉴定。识别后,根据颜色选择进行聚类分析和相似度评价。该研究作为海报在第十届国际蚜虫研讨会(2017年9月4 - 8日,卡帕多西亚,土耳其)上发表。
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引用次数: 0
Determination of Plant Parasitic Nematodes on Oil Rose Growing Areas in Isparta Province of Türkiye 伊斯巴达省油玫瑰种植区植物寄生线虫的测定
Pub Date : 2023-03-14 DOI: 10.16955/bitkorb.1244955
Fatma Gül Göze Özdemir
The study was carried out to investigate plant parasitic nematodes in oil rose growing areas in Isparta province in July-August 2022. Soil samples were taken from 15 different areas and the study was carried out with 65 samples. A total of 12 species of plant parasitic nematodes were determined in the study. These are Tylenchus spp., Aphelenchus spp., Pratylenchus spp., Dictylenchus spp., Dorylaimus spp., Paratylenchus spp., Longidorus spp., Xiphinema spp., Meloidogyne spp., Helicotylenchus spp. and Merlinus spp. The Pratylenchus (52.3%), Paratylenchus (38.5%) and Helicotylenchus (41.5%) seemed to be the most prevailing genera. The least common genus is Meloidogyne (7.6%). Root knot nematode species molecular identification was determined by species-specific primers from larvae. The 5 samples taken from cultivated oil rose fields were found to be infected and were detected with Meloidogyne hapla Chitwood, 1949. Densities of M. hapla varied between 60-480/100 g soil. This is the first report identification of M. hapla on oil rose in Türkiye.
本研究于2022年7 - 8月对伊斯帕塔省油玫瑰种植区的植物寄生线虫进行了调查。土壤样本取自15个不同地区,共65个样本。本研究共鉴定出12种植物寄生线虫。主要属有:Tylenchus、Aphelenchus、Pratylenchus、Dictylenchus、Dorylaimus、副atyllenchus、Longidorus、Xiphinema、Meloidogyne、Helicotylenchus和Merlinus,其中Pratylenchus(52.3%)、副atyllenchus(38.5%)和Helicotylenchus(41.5%)。最不常见的属是Meloidogyne(7.6%)。利用根结线虫幼虫的种特异性引物对根结线虫进行分子鉴定。从栽培的油玫瑰田采集的5份样品中发现了侵染,并检出了1949年的赤木病。密度在60 ~ 480株/100 g土壤之间变化。这是首次报道鉴定出基耶油玫瑰上的hapla。
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引用次数: 0
Siyah Çayın Sülfiril Florür İle Fumugasyonu Sonrası Florür İyonu Kalıntısının Değerlendirilmesi
Pub Date : 2023-03-14 DOI: 10.16955/bitkorb.1241447
Üzeyir Aktuğ, Yalçın Duydu
Sulfuryl fluoride is a colorless, odorless gas used as a fumigant against pests during the storage of agricultural products. It is also one of the alternatives to Methyl bromide, which is banned by the Montreal Protocol. During a fumigation, the decomposition product of sulfuryl fluoride residue can also be formed in addition to the sulfuryl fluoride residue. Fluoride is actually a natural constituent of the tea plant. However, within the growing process of the tea plant fluoride is concentrated in the old leaves when compared with the young shoots. Investigating the variation of the fluoride content in black tea before and after sulfuryl fluoride fumigation is the subject of this study. Three different brands black teas with different fluoride concentrations purchased from local supermarkets in 2022 were fumigated with approximately 60 g/m3 of sulfuryl fluoride for 24 hours in a 1 m3 fumigation chamber. The AOAC’s recommended method for extraction of fluoride in plants (Method 975.04) was applied in this study. It was compared with the fluoride contents after fumigation in teas (41.4; 165.1 and 329.5 mg/kg, respectively). It is concluded that there is no statistically [t(7)=0,284; t(7)=0,769 and t(7)=1,419 p>0,05] significant difference in fluoride content in teas after fumigation.
硫酰氟是一种无色无味的气体,在农产品储存过程中用作防虫熏蒸剂。它也是《蒙特利尔议定书》禁止使用的甲基溴的替代品之一。在熏蒸过程中,除硫酰氟残留物外,还可形成硫酰氟残留物的分解产物。氟化物实际上是茶树的天然成分。然而,在茶树的生长过程中,与幼芽相比,氟化物集中在老叶中。研究硫酰氟熏蒸前后红茶中氟含量的变化。研究人员于2022年从当地超市购买了三种不同品牌、不同氟浓度的红茶,在1立方米的熏蒸室中,以约60克/立方米的硫酰氟熏蒸24小时。本研究采用AOAC推荐的植物中氟化物提取方法(方法975.04)。与熏蒸后茶叶中氟含量(41.4;分别为165.1和329.5 mg/kg)。结果表明,在统计学上不存在[t(7)=0,284;T(7)= 0.769和T (7)= 1419 (p> 0.05)对熏蒸后茶叶中氟含量有显著性差异。
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引用次数: 0
Yeni ve tüm bilinen konukçu bitkileri ve yayılış verileri ile birlikte Türkiye'deki Xylosteini Reitter, 1913 (Coleoptera: Cerambycidae: Lepturinae) kabilesi içindeki ilkel ve nadir orman zararlıları üyelerinin beslenme tercihleri
Pub Date : 2023-03-12 DOI: 10.16955/bitkorb.1233521
Hüseyin Özdikmen, N. Bal
The detection of cerambycids and their feeding preferences within the natural ecosystems is of great ecological and economic importance. Accordingly, the paper presents collectively all taxa of the primitive, rare and hardly studied tribe Xylosteini Reitter in Turkey with new data on their feeding preferences and distribution patterns for the first time. The available specimens were collected from localities in Artvin, Bolu, Gümüşhane, Kırklareli, Samsun and Sivas provinces of Turkey in 2002, 2008, 2009, 2010, 2011, 2022. As a result, it is determined that Turkish Xylosteini includes four species of two genera. All of them are rare species. Abies nordmanniana (Pinaceae) and Fagus orientalis (Fagaceae) are determined as new host plant species for Xylosteus kadleci Miroshnikov. Accordingly, at least five or six host plants for each species are determined with the present study. According to the present study, the members of the genus Leptorhabdium Kraatz are preferred deciduous trees only, while the members of the genus Xylosteus Frivaldszky von Frivald are preferred both deciduous and also coniferous trees. Besides, detailed distribution data with figures show provincial and regional distribution patterns of all species are also given. Apparently, the genus Leptorhabdium seems to be represented only by L. caucasicum in North-Eastern Anatolia of Turkey, while the genus Xylosteus seems to be represented by X. spinolae in European Turkey (=Thrace), by X. kadleci in North-Western Anatolia of Turkey, and by X. caucasicola in North-Eastern Anatolia of Turkey.
在自然生态系统中检测天牛及其取食偏好具有重要的生态和经济意义。据此,本文首次对土耳其原始的、罕见的、几乎没有被研究过的Xylosteini Reitter部落的所有分类群进行了综合介绍,并对其摄食偏好和分布格局进行了新的研究。现有标本于2002年、2008年、2009年、2010年、2011年和2022年在土耳其阿尔特文、博卢、g 哈内、Kırklareli、萨姆松和锡瓦斯省采集。因此,确定土耳其木虱包括2属4种。它们都是稀有物种。确定了北方冷杉(松科)和东方Fagus orientalis(壳斗科)为新寄主植物。因此,本研究至少确定了每个物种的5 - 6种寄主植物。根据目前的研究,Leptorhabdium Kraatz属的成员只偏爱落叶乔木,而Xylosteus Frivaldszky von Frivald属的成员既喜欢落叶乔木,也喜欢针叶树。此外,还提供了详细的分布数据,并用图形显示了各树种的省、区分布格局。显然,Leptorhabdium属似乎只在土耳其安纳托利亚东北部由L. caucasicum代表,而Xylosteus属似乎在土耳其欧洲(色雷斯)由X. spinolae代表,在土耳其安纳托利亚西北部由X. kadleci代表,在土耳其安纳托利亚东北部由X. caucasicola代表。
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引用次数: 0
Determination of Suppression Efficiency of Vermicompost Extracts on Some Aerial Fungal Plant Pathogens 蚯蚓堆肥提取物对几种空气真菌植物病原菌抑菌效果的测定
Pub Date : 2023-03-09 DOI: 10.16955/bitkorb.1238852
Servet Uzunok
The use of vermicompost in agriculture has become increasingly common, especially in organic agriculture. In this research, the suppression efficiency of horse and cattle vermicompost extracts against some aerial fungal plant pathogens (Monilinia laxa, Cytospora leucostoma, Botryosphaeria obtusa, Phomopsis viticola, Alternaria mali, and Botrytis cinerea) that damage fruit trees and are common in fruit production areas, was studied for the first time in our country. %25, %50, %75, and %100 concentrations of horse and cattle extracts were tested against fungal plant isolates. The results were evaluated by two-way ANOVA testing the suppression rates of vermicompost extracts in petri plates. While the best results were found in M. laxa (90,6%), P. viticola (80,2%), A. mali (65,1%) at 100% concentration for horse and cattle vermicomposts respectively, the suppression of B. obtusa (44,7%), C. leucostoma (38,1%), and B. cinerea (35,5%) was found to be lower.
蚯蚓堆肥在农业中的应用越来越普遍,特别是在有机农业中。本研究在国内首次研究了马、牛蚯蚓堆肥提取物对水果产区常见的几种危害果树的空气真菌植物病原菌(Monilinia laxa、Cytospora leucostoma、Botryosphaeria obtusa、Phomopsis viticola、Alternaria mali和Botrytis cinerea)的抑制效果。对%25、%50、%75和%100浓度的马和牛提取物进行了抗真菌植物分离物试验。采用双因素方差分析对培养皿中蚯蚓堆肥提取物的抑菌率进行评价。马和牛蚯蚓堆肥在100%浓度下,分别对laxa(91.6%)、P. viticola(80.2%)、A. mali(61.5%)的抑制效果最好,对B. obtusa(44.7%)、C. leucostoma(38.1%)和B. cinerea(35.5%)的抑制效果较差。
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引用次数: 1
Management of Dısease Complex of Meloidogyne incognita and Fusarium oxysporum f.sp. radicis lycopersici on Tomato Usıng Some Essentıal Oıls 隐丝虫病与尖孢镰刀菌Dısease复合体的防治。番茄根霉Usıng有的Essentıal Oıls
Pub Date : 2022-12-13 DOI: 10.16955/bitkorb.1172169
Fatma Gül Göze Özdemir
The effects of commercial thyme, sage, garlic, sesame, rosemary, lemon and mustard essential oils (Botalife Natural and Aromatic Products Ltd. Şti, Turkey) on disease severity were investigated in simultaneous inoculation of Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949 and Fusarium oxysporum f.sp.radicis lycopersici (Jarvis & Shoemaker) on tomato. Nematicide (Velum®, Fluopyram) and fungicide (Cebir®, Fludioxonil + Metalaxyl) were used as positive controls. Negative control was only plants with nematode and fungus inoculation. The study was set up in a randomized plot design with 5 replications for each essential oil. In simultaneous inoculations, 1000 M. incognita second juvenile larvaes/1ml and 3X106 spore/ml FORL were used for each seedling. The essential oil applications were applied to the soil at a dose of 1000 ppm for each pot, one day after the nematode and fungus inoculation. The study was terminated after 60 days, and the assestment was based on gall, egg mass and disease severity. Fungal growth and nematode development on roots was found lower in all tested oils applications than negative control but fungisidal and nematicidal activity varied. Thyme and garlic essential oils had the highest control effect on nematode and fungus with 55.20% in simultaneous inoculation and this effect was higher than only nematicide (38.84%) and only fungicide (33.20%) applications. Sage (38.84%), rosemary (33.28%) and mustard (38.92%) essential oils were found to suppress disease severity higher than sesame (22.16%) and lemon (22.16%). It has been determined that thyme and garlic essential oils are good alternatives to manage root knot nematode and FORL disease complexes.
用百里香、鼠尾草、大蒜、芝麻、迷迭香、柠檬和芥菜精油(Botalife Natural and Aromatic Products Ltd. Şti,土耳其)同时接种番茄上的梅氏菌(Meloidogyne incognita, Kofoid and White, 1919)、奇特木(Chitwood, 1949)和番茄枯萎菌(Fusarium oxysporum f.sp.radicis lycopersici, Jarvis & Shoemaker),研究了这些精油对疾病严重程度的影响。杀线虫剂(Velum®、Fluopyram)和杀菌剂(Cebir®、Fludioxonil + metaxyl)作为阳性对照。阴性对照仅为接种线虫和真菌的植株。本研究采用随机分组设计,每种精油5个重复。同时接种时,每株幼苗接种1000只黑斑夜蛾二幼幼虫/1ml,每株接种3X106个孢子/ml FORL。在线虫和真菌接种后一天,以1000ppm的剂量将精油应用于土壤中。研究在60天后终止,并根据胆汁、卵子数量和疾病严重程度进行评估。与阴性对照相比,所有施用油脂的根上真菌生长和线虫发育均较低,但杀真菌和杀线虫活性各不相同。百里香和大蒜精油对线虫和真菌的防治效果最高,同时接种效果为55.20%,高于单独施用杀线虫剂(38.84%)和单独施用杀菌剂(33.20%)。鼠尾草(38.84%)、迷迭香(33.28%)和芥末(38.92%)精油对疾病严重程度的抑制作用高于芝麻(22.16%)和柠檬(22.16%)。已经确定百里香和大蒜精油是管理根结线虫和FORL疾病复合体的良好替代品。
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引用次数: 1
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Bitki Koruma Bülteni
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