Pub Date : 2021-09-23DOI: 10.22038/JMDS.2021.55804.2012
S. S. Hendi, Hanieh Haghighi, Shahriar Shahriari
Introduction: Enterococcus faecalis (E. faecalis) is one of the microbes of the normal flora of the oral cavity, which is one of the common factors in the failure of root canal treatment. This in vitro study aimed to evaluate the ability of barberry extract to prevent the formation of microbial biofilm by E. faecalis. Materials and Methods: Initially, E. faecalis was cultured simultaneously with barberry bark and root extract, and biofilm formation was evaluated after 48 h. In the second stage of the active culture of the bacteria (48 h), all root and bark concentrations had similar growth inhibition with the control group. The same results were observed after two weeks. Fluorescence staining with SYPRO® Ruby (Invitrogen, USA, 2010) was used to confirm the formation of biofilm. Subsequently, all samples were examined by fluorescence microscope, and the results were reported using Relative Fluorescence Unit. Results: In the simultaneous cultivation of barberry and E. faecalis, root (30%), growth medium (50%), and bark (30%) had similar growth inhibition with the control group. After adding barberry bark and root extract (48 h), all concentrations of barberry bark and root showed similar growth inhibition with the control group. After two weeks of E. faecalis culture, the results were the same as those of the first group. However, wide and thick biofilms were formed in the samples without treatment and those treated with normal saline. Conclusion: Due to the positive effects of barberry bark and root extract, it can be used to sterilize dental canals and can be considered a suitable alternative to hypochlorite.
{"title":"Evaluation of the Ability of Barberry Liquid Extract to Prevent the Formation of Microbial Biofilm by Enterococcus Faecalis: An In Vitro Study","authors":"S. S. Hendi, Hanieh Haghighi, Shahriar Shahriari","doi":"10.22038/JMDS.2021.55804.2012","DOIUrl":"https://doi.org/10.22038/JMDS.2021.55804.2012","url":null,"abstract":"Introduction: Enterococcus faecalis (E. faecalis) is one of the microbes of the normal flora of the oral cavity, which is one of the common factors in the failure of root canal treatment. This in vitro study aimed to evaluate the ability of barberry extract to prevent the formation of microbial biofilm by E. faecalis. Materials and Methods: Initially, E. faecalis was cultured simultaneously with barberry bark and root extract, and biofilm formation was evaluated after 48 h. In the second stage of the active culture of the bacteria (48 h), all root and bark concentrations had similar growth inhibition with the control group. The same results were observed after two weeks. Fluorescence staining with SYPRO® Ruby (Invitrogen, USA, 2010) was used to confirm the formation of biofilm. Subsequently, all samples were examined by fluorescence microscope, and the results were reported using Relative Fluorescence Unit. Results: In the simultaneous cultivation of barberry and E. faecalis, root (30%), growth medium (50%), and bark (30%) had similar growth inhibition with the control group. After adding barberry bark and root extract (48 h), all concentrations of barberry bark and root showed similar growth inhibition with the control group. After two weeks of E. faecalis culture, the results were the same as those of the first group. However, wide and thick biofilms were formed in the samples without treatment and those treated with normal saline. Conclusion: Due to the positive effects of barberry bark and root extract, it can be used to sterilize dental canals and can be considered a suitable alternative to hypochlorite.","PeriodicalId":30335,"journal":{"name":"Journal of Mashhad Dental School","volume":"45 1","pages":"289-298"},"PeriodicalIF":0.0,"publicationDate":"2021-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45442634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-11DOI: 10.22038/JMDS.2021.54176.1988
S. Razavi, S. Khalesi, Mahsa Chamlanian
Introduction: The lifestyle of people is influenced by various factors, such as common beliefs in society. Analysis of the oral health behaviors of people helps to set the correct behavioral correction strategies. In order to establish a proper relationship between healthcare providers and patients, it is necessary to have a correct understanding of peoples' beliefs in this regard. This study aimed to investigate the attitudes of patients referring to dental centers towards some common beliefs in the field of oral health. Materials and Methods: This descriptive cross-sectional study included 392 patients referring to different dental treatment centers in Isfahan, Iran, during 2020. The patients were then selected using the two-stage random cluster sampling method. A researcher-made questionnaire was prepared by reviewing previous studies and professors' opinions. After obtaining the reliability and validity of the tool, it was used to assess the patients' attitudes. The questionnaire was then completed by the patients in person. The collected data were statistically analyzed in SPSS software (version 22) using t-test, Pearson correlation coefficient, Spearman correlation coefficient, one-way analysis of variance, and Tukey test. A p-value less than 0.05 was considered statistically significant. Results: The mean score of the patients' attitude was obtained at 3.15±0.29. There was no significant difference between males and females regarding the mean score of attitudes. Furthermore, no significant relationship was observed between the mean score of the patients' attitudes and their age (P=0.486). However, there was a significant relationship between the mean score of the patients' attitude and their education level (P=0.001). Conclusion: According to the results, half of the patients had a moderate level of attitude. This indicates the need to inform and educate people, as well as change their attitude towards receiving oral health services. In addition, national media can play a more prominent role in achieving this goal.
{"title":"Investigation of the Patients' Attitudes Referring To Dental Centers toward Some Common Beliefs about Oral Health in Isfahan, Iran, During 2020","authors":"S. Razavi, S. Khalesi, Mahsa Chamlanian","doi":"10.22038/JMDS.2021.54176.1988","DOIUrl":"https://doi.org/10.22038/JMDS.2021.54176.1988","url":null,"abstract":"Introduction: The lifestyle of people is influenced by various factors, such as common beliefs in society. Analysis of the oral health behaviors of people helps to set the correct behavioral correction strategies. In order to establish a proper relationship between healthcare providers and patients, it is necessary to have a correct understanding of peoples' beliefs in this regard. This study aimed to investigate the attitudes of patients referring to dental centers towards some common beliefs in the field of oral health. Materials and Methods: This descriptive cross-sectional study included 392 patients referring to different dental treatment centers in Isfahan, Iran, during 2020. The patients were then selected using the two-stage random cluster sampling method. A researcher-made questionnaire was prepared by reviewing previous studies and professors' opinions. After obtaining the reliability and validity of the tool, it was used to assess the patients' attitudes. The questionnaire was then completed by the patients in person. The collected data were statistically analyzed in SPSS software (version 22) using t-test, Pearson correlation coefficient, Spearman correlation coefficient, one-way analysis of variance, and Tukey test. A p-value less than 0.05 was considered statistically significant. Results: The mean score of the patients' attitude was obtained at 3.15±0.29. There was no significant difference between males and females regarding the mean score of attitudes. Furthermore, no significant relationship was observed between the mean score of the patients' attitudes and their age (P=0.486). However, there was a significant relationship between the mean score of the patients' attitude and their education level (P=0.001). Conclusion: According to the results, half of the patients had a moderate level of attitude. This indicates the need to inform and educate people, as well as change their attitude towards receiving oral health services. In addition, national media can play a more prominent role in achieving this goal.","PeriodicalId":30335,"journal":{"name":"Journal of Mashhad Dental School","volume":"45 1","pages":"247-256"},"PeriodicalIF":0.0,"publicationDate":"2021-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45498110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-22DOI: 10.22038/JMDS.2021.51282.1940
Samaneh Hemmati, P. Kermani, J. Cherati, Haleh Hali
Introduction: Severe early childhood caries is an acute type of dental caries which causes adverse effects on the quality of nourishment and growth of the baby. The purpose of this study was to determine any relationship between s-ECC and BMI in children aged 3-6 years old of sari`s kindergartens. Materials and Methods: In this descriptive study 401 systematically healthy children aged 3-6 years old, were selected randomly from sari kindergartens. After completing the questionnaire, variables such as height, weight and dmft (according to WHO`s standard) were recorded in a checklistand the relationship between BMI and s-ECC was investigated. Data were subjected to Chi-square and Logistic regression tests. Result: Totally 188 children (46.8%) suffered from s-ECC and 213 (53%) were not affected by. Also, 38.1% had normal status, 28.1% were preoverweight, 19.7% were over weighted and 13.7% were underweighted. Statistical Society had mean dmft of 3.5 and mean age of 4.7. According to the data analysis, age, BMI status (obesity and weight deficiency), milk intake during sleep and consumption of snacks have significant effect on incidence of s-ECC. (Pvalue< 0.001) Conclusion: The results of the current study indicate a positive relationship between s-ECC and obesity and weight loss based on the BMI status chart of the disease control center.
{"title":"The Relationship between Severe Early Childhood Caries and BMI in 3-6-Year-Old Children","authors":"Samaneh Hemmati, P. Kermani, J. Cherati, Haleh Hali","doi":"10.22038/JMDS.2021.51282.1940","DOIUrl":"https://doi.org/10.22038/JMDS.2021.51282.1940","url":null,"abstract":"Introduction: Severe early childhood caries is an acute type of dental caries which causes adverse effects on the quality of nourishment and growth of the baby. The purpose of this study was to determine any relationship between s-ECC and BMI in children aged 3-6 years old of sari`s kindergartens. Materials and Methods: In this descriptive study 401 systematically healthy children aged 3-6 years old, were selected randomly from sari kindergartens. After completing the questionnaire, variables such as height, weight and dmft (according to WHO`s standard) were recorded in a checklistand the relationship between BMI and s-ECC was investigated. Data were subjected to Chi-square and Logistic regression tests. Result: Totally 188 children (46.8%) suffered from s-ECC and 213 (53%) were not affected by. Also, 38.1% had normal status, 28.1% were preoverweight, 19.7% were over weighted and 13.7% were underweighted. Statistical Society had mean dmft of 3.5 and mean age of 4.7. According to the data analysis, age, BMI status (obesity and weight deficiency), milk intake during sleep and consumption of snacks have significant effect on incidence of s-ECC. (Pvalue< 0.001) Conclusion: The results of the current study indicate a positive relationship between s-ECC and obesity and weight loss based on the BMI status chart of the disease control center.","PeriodicalId":30335,"journal":{"name":"Journal of Mashhad Dental School","volume":"45 1","pages":"188-195"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48291472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-21DOI: 10.22038/JMDS.2021.49442.1920
Parastoo Namdar, A. Shiva, Mehran Armin, Tahura Etezadi, R. Mohammadpour, Ali Ajilian
Introduction: Third molar teeth are the most common teeth with agenesis. The absence of this tooth also increases the probability of anomalies in other teeth by several times. Since panoramic radiography is used to diagnose and plan treatment in orthodontic patients, along with routine clinical examination, this study aimed to determine the prevalence of third molar agenesis and incidence of other associated anomalies in comparison to that reported for the non-agenesis group using panoramic radiographs. Materials and Methods: In this descriptive-analytical study, the pretreatment records of 562 patients were randomly collected. Out of these subjects, 538 patients were selected based on inclusion and exclusion criteria. The panoramic radiographic images of patients who did not have at least one third molar were assessed with regard to the presence of other dental anomalies, such as dental agenesis, hypodontia, hyperdontia, impacted canine, peg-shaped lateral incisors, ectopic eruption, microdontia, dilaceration, transposition, and transmigration. The prevalence of these anomalies was compared to that of a randomized control group selected from patients with the presence of each four third molars. Results: Out of 538 studied panoramic radiographs, overall, 13.7%, 13.5%, 13.4%, 5.9%, 2.7%, and 1.4% of them had at least one third molar agenesis, canine impaction, peg-shaped lateral incisors, missing teeth, transposition, and hyperdontia, respectively. The frequency rates of impaction and missing teeth were higher in the agenesis group (30% and 40.5%) than those reported for the control group (16% and 2.7%) (p >0.001), respectively. The presence or absence of other dental anomalies was not statistically significant between the two groups and two genders. In addition, the present study demonstrated that the probability of the presence of at least one type of other dental anomalies was higher in individuals with third molar agenesis in comparison to that reported for the control group (p >0.001). Conclusion: Among different types of dental anomalies in the studied population, the probability of impaction and missing teeth was higher in the third molar agenesis group than that of the control group. Furthermore, the prevalence of third molar agenesis was not different between the two genders.
{"title":"Association between Third Molar Agenesis and Other Dental Anomalies","authors":"Parastoo Namdar, A. Shiva, Mehran Armin, Tahura Etezadi, R. Mohammadpour, Ali Ajilian","doi":"10.22038/JMDS.2021.49442.1920","DOIUrl":"https://doi.org/10.22038/JMDS.2021.49442.1920","url":null,"abstract":"Introduction: Third molar teeth are the most common teeth with agenesis. The absence of this tooth also increases the probability of anomalies in other teeth by several times. Since panoramic radiography is used to diagnose and plan treatment in orthodontic patients, along with routine clinical examination, this study aimed to determine the prevalence of third molar agenesis and incidence of other associated anomalies in comparison to that reported for the non-agenesis group using panoramic radiographs. Materials and Methods: In this descriptive-analytical study, the pretreatment records of 562 patients were randomly collected. Out of these subjects, 538 patients were selected based on inclusion and exclusion criteria. The panoramic radiographic images of patients who did not have at least one third molar were assessed with regard to the presence of other dental anomalies, such as dental agenesis, hypodontia, hyperdontia, impacted canine, peg-shaped lateral incisors, ectopic eruption, microdontia, dilaceration, transposition, and transmigration. The prevalence of these anomalies was compared to that of a randomized control group selected from patients with the presence of each four third molars. Results: Out of 538 studied panoramic radiographs, overall, 13.7%, 13.5%, 13.4%, 5.9%, 2.7%, and 1.4% of them had at least one third molar agenesis, canine impaction, peg-shaped lateral incisors, missing teeth, transposition, and hyperdontia, respectively. The frequency rates of impaction and missing teeth were higher in the agenesis group (30% and 40.5%) than those reported for the control group (16% and 2.7%) (p >0.001), respectively. The presence or absence of other dental anomalies was not statistically significant between the two groups and two genders. In addition, the present study demonstrated that the probability of the presence of at least one type of other dental anomalies was higher in individuals with third molar agenesis in comparison to that reported for the control group (p >0.001). Conclusion: Among different types of dental anomalies in the studied population, the probability of impaction and missing teeth was higher in the third molar agenesis group than that of the control group. Furthermore, the prevalence of third molar agenesis was not different between the two genders.","PeriodicalId":30335,"journal":{"name":"Journal of Mashhad Dental School","volume":"45 1","pages":"83-92"},"PeriodicalIF":0.0,"publicationDate":"2021-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44356088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-21DOI: 10.22038/JMDS.2021.53397.1968
S. Shotorbani, Adel Espotin, J. Mahmoudi, A. Dehghan, S. S. Eteghad
Introduction: According to previous studies, heat shock protein 70 (HSP70) plays a role in the production of proinflammatory cytokines and inflammation. Given that no study has been performed in the field of dentistry in this regard, the present research aimed to identify the effect of HSP70 on moderate to severe generalized chronic periodontitis with the increase of mitogen-activated protein kinase (MAPK) and toll-like receptor 4 (TLR4) signaling pathways. Materials and Methods: This pilot study was performed on 50 subjects with moderate to severe chronic generalized periodontitis and 50 subjects with healthy periodontitis who were candidates for crown lengthening (CL) surgery. The subjects were selected based on the inclusion criteria from the patients who referred to the Gingival Surgery Department in a Private Dental Center, Tabriz, Iran. Tissue samples were obtained from the patients during pocket depth reduction surgery (for the experimental group) and CL surgery (for the control group). Macrophage inflammatory cells were extracted from tissue samples and the cells were stimulated by HSP70 as a timer; subsequently, the level of TLR4 in macrophage cells was examined. Results of the study were reported using descriptive statistical methods, such as mean, standard deviation, and frequency percentage. Repeated measures analysis was used to compare the expression of TLR4 in nuclear factor kappa B (NF-KB) and MAPK pathways at different hours. Moreover, ANOVA analysis of covariance was used to compare this rate between these two pathways at different times. Statistical analysis was performed in SPSS software (version 17) and a p -value of Results: Based on the results, there was a significant relationship between TLR4 and HSP70 (p <0.0001). It was also found that TLR4, MAPK, and NF-KB levels increased in the presence of HSP70. Conclusion: According to the findings, it can be said that TLR4 expression levels increased in the presence of HSP70 in periodontitis and can increase even more by excitation of MAPK and NF-KB pathways.
前期研究发现，热休克蛋白70 (heat shock protein 70, HSP70)在促炎细胞因子的产生和炎症反应中发挥作用。鉴于目前在牙科领域尚未有相关研究，本研究旨在确定HSP70通过增加丝裂原活化蛋白激酶(MAPK)和toll样受体4 (TLR4)信号通路对中重度广泛性慢性牙周炎的影响。材料和方法:本初步研究纳入了50名中重度慢性广泛性牙周炎患者和50名健康牙周炎患者，这些患者都是牙冠延长(CL)手术的候选人。受试者是根据纳入标准从转诊到伊朗大不里士(Tabriz)一家私人牙科中心牙龈外科的患者中选择的。实验组和对照组分别进行口袋深度缩小手术和CL手术。从组织样本中提取巨噬细胞炎性细胞，用HSP70刺激细胞作为计时器;随后检测巨噬细胞中TLR4的表达水平。研究结果采用描述性统计方法报告，如平均值、标准差和频率百分比。采用重复测量分析比较不同时间TLR4在核因子κ B (NF-KB)和MAPK通路中的表达。此外，采用协方差的方差分析来比较这两种途径在不同时间的这一比率。采用SPSS软件(version 17)进行统计学分析，结果的p值为:基于结果，TLR4与HSP70之间存在显著相关(p <0.0001)。我们还发现，在HSP70存在的情况下，TLR4、MAPK和NF-KB水平升高。结论:根据研究结果，可以说在牙周炎中HSP70存在时，TLR4的表达水平升高，并且可以通过激发MAPK和NF-KB途径进一步升高。
{"title":"Effects of HSP70 on TLR4 Expression by Increasing MAPK and NF-KB Signaling Pathways in Periodontitis","authors":"S. Shotorbani, Adel Espotin, J. Mahmoudi, A. Dehghan, S. S. Eteghad","doi":"10.22038/JMDS.2021.53397.1968","DOIUrl":"https://doi.org/10.22038/JMDS.2021.53397.1968","url":null,"abstract":"Introduction: According to previous studies, heat shock protein 70 (HSP70) plays a role in the production of proinflammatory cytokines and inflammation. Given that no study has been performed in the field of dentistry in this regard, the present research aimed to identify the effect of HSP70 on moderate to severe generalized chronic periodontitis with the increase of mitogen-activated protein kinase (MAPK) and toll-like receptor 4 (TLR4) signaling pathways. Materials and Methods: This pilot study was performed on 50 subjects with moderate to severe chronic generalized periodontitis and 50 subjects with healthy periodontitis who were candidates for crown lengthening (CL) surgery. The subjects were selected based on the inclusion criteria from the patients who referred to the Gingival Surgery Department in a Private Dental Center, Tabriz, Iran. Tissue samples were obtained from the patients during pocket depth reduction surgery (for the experimental group) and CL surgery (for the control group). Macrophage inflammatory cells were extracted from tissue samples and the cells were stimulated by HSP70 as a timer; subsequently, the level of TLR4 in macrophage cells was examined. Results of the study were reported using descriptive statistical methods, such as mean, standard deviation, and frequency percentage. Repeated measures analysis was used to compare the expression of TLR4 in nuclear factor kappa B (NF-KB) and MAPK pathways at different hours. Moreover, ANOVA analysis of covariance was used to compare this rate between these two pathways at different times. Statistical analysis was performed in SPSS software (version 17) and a p -value of Results: Based on the results, there was a significant relationship between TLR4 and HSP70 (p <0.0001). It was also found that TLR4, MAPK, and NF-KB levels increased in the presence of HSP70. Conclusion: According to the findings, it can be said that TLR4 expression levels increased in the presence of HSP70 in periodontitis and can increase even more by excitation of MAPK and NF-KB pathways.","PeriodicalId":30335,"journal":{"name":"Journal of Mashhad Dental School","volume":"45 1","pages":"63-69"},"PeriodicalIF":0.0,"publicationDate":"2021-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43907639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-03-21DOI: 10.22038/JMDS.2021.44119.1899
Shadi Saghafi Khadem, S. M. Mirhashemi, Masoomeh Saeidi Robat
Introduction: Lichen planus is a relatively common chronic inflammatory autoimmune disease of unknown etiology. The World Health Organization has identified lichen planus as a potential precancerous lesion. Cyclooxygenase-2 (COX-2) is a key enzyme for inflammatory processes and cell proliferation. The increased expression of COX-2 in some chronic precancerous inflammatory diseases and malignant neoplasms of the upper aerodigestive tract suggests its role in the early stages of carcinogenesis of the head and neck and can show its potential as a prognostic factor and marker of altered behavior. The aim of this study was to analyze the COX-2 marker in the pathogenesis and biological behavior of oral lichen planus and oral lichen planus with dysplasia. Materials and Methods: The incidence rate of the COX-2 marker in the 50 samples of the pathology archive of Mashhad Dental School, Mashad, Iran, including 25 samples of oral lichen planus and 25 samples of oral lichen planus with dysplasia, was evaluated using immunohistochemistry in 2017. In this cross-sectional descriptive-analytical study, the Kruskal-Wallis and Chi-square statistical tests were used to compare the incidence of the COX-2 marker in the study groups. Results: Based on the results of this study, there was no significant difference between the two groups of oral lichen planus and oral lichen planus with dysplasia in COX-2 staining pattern in the basal layer and connective tissue; however, it was significantly higher in the suprabasal layer of the lichen planus group with dysplasia than that of the oral lichen planus group. In terms of location, lichen planus lesions with dysplasia were more commonly observed in the buccal mucosa, and oral lichen planuslesions (without dysplasia) were more frequently noticed in lips. Conclusion: Based on the findings of the present study, it can be concluded that increased COX-2 expression is observed in oral lichen planus lesions, especially those with dysplasia. The presence of lesions in some areas of the mouth, such as the buccal mucosa, tongue, and border of the tongue, is also more associated with dysplasia.
{"title":"Evaluation of Cyclooxygenase-2 in Oral Lichen Planus Using Immunohistochemistry","authors":"Shadi Saghafi Khadem, S. M. Mirhashemi, Masoomeh Saeidi Robat","doi":"10.22038/JMDS.2021.44119.1899","DOIUrl":"https://doi.org/10.22038/JMDS.2021.44119.1899","url":null,"abstract":"Introduction: Lichen planus is a relatively common chronic inflammatory autoimmune disease of unknown etiology. The World Health Organization has identified lichen planus as a potential precancerous lesion. Cyclooxygenase-2 (COX-2) is a key enzyme for inflammatory processes and cell proliferation. The increased expression of COX-2 in some chronic precancerous inflammatory diseases and malignant neoplasms of the upper aerodigestive tract suggests its role in the early stages of carcinogenesis of the head and neck and can show its potential as a prognostic factor and marker of altered behavior. The aim of this study was to analyze the COX-2 marker in the pathogenesis and biological behavior of oral lichen planus and oral lichen planus with dysplasia. Materials and Methods: The incidence rate of the COX-2 marker in the 50 samples of the pathology archive of Mashhad Dental School, Mashad, Iran, including 25 samples of oral lichen planus and 25 samples of oral lichen planus with dysplasia, was evaluated using immunohistochemistry in 2017. In this cross-sectional descriptive-analytical study, the Kruskal-Wallis and Chi-square statistical tests were used to compare the incidence of the COX-2 marker in the study groups. Results: Based on the results of this study, there was no significant difference between the two groups of oral lichen planus and oral lichen planus with dysplasia in COX-2 staining pattern in the basal layer and connective tissue; however, it was significantly higher in the suprabasal layer of the lichen planus group with dysplasia than that of the oral lichen planus group. In terms of location, lichen planus lesions with dysplasia were more commonly observed in the buccal mucosa, and oral lichen planuslesions (without dysplasia) were more frequently noticed in lips. Conclusion: Based on the findings of the present study, it can be concluded that increased COX-2 expression is observed in oral lichen planus lesions, especially those with dysplasia. The presence of lesions in some areas of the mouth, such as the buccal mucosa, tongue, and border of the tongue, is also more associated with dysplasia.","PeriodicalId":30335,"journal":{"name":"Journal of Mashhad Dental School","volume":"45 1","pages":"12-21"},"PeriodicalIF":0.0,"publicationDate":"2021-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48925255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-22DOI: 10.22038/JMDS.2018.11989
Shadi Saghafi Khadem, Reza Zare-Mahmoodabadi, F. Mohajertehran, F. Jafari, Maede Shokri
Introduction: Ameloblastoma is a prevalent odontogenic maxillofacial tumor. Human papillomavirus (HPV) is considered to be one of the main risk factors for ameloblastoma. The current research aimed to investigate the presence of HPV in the ameloblastoma of jaw samples using the polymerase chain reaction (PCR). Materials and Methods: This study was conducted on 77 formalin-fixed and paraffin-embedded tissue blocks, which were obtained from the archives of the Department of Pathology at Mashhad Dentistry School and Ghaem Hospital in Mashhad, Iran. Deparaffinization and DNA extraction in the samples were performed in accordance with the instructions of the YTA genomic DNA extraction mini kit. After PCR, the presence of HPV was evaluated in the samples. Data analysis was performed in SPSS version 16 using descriptive statistic (frequency distribution tables and charts), Chi-square, and logistic regression. Results: Among 77 lesions, 20 cases were follicular, four cases were acanthomatous, three cases were basal cells, eight cases were desmoblastic, 17 cases were unicystic, and 25 cases were plexiform. Moreover, five samples (6.5) were positive for HPV. HPV was detected in one follicular case, two unicystic cases, and two plexiform cases. Conclusion: Considering the low prevalence of HPV in the examined lesions, it could not be concluded that the virus was involved in the etiology and pathogenesis of the lesions. Therefore, further investigations must be conducted on larger sample sizes for more accurate results.
成釉细胞瘤是一种常见的牙源性颌面部肿瘤。人乳头瘤病毒(HPV)被认为是成釉细胞瘤的主要危险因素之一。本研究旨在利用聚合酶链反应(PCR)研究HPV在颌骨成釉细胞瘤样本中的存在。材料和方法:本研究选取了77个福尔马林固定和石蜡包埋的组织块，这些组织块来自伊朗马什哈德牙科学校病理学系和Ghaem医院的档案。按照YTA基因组DNA提取迷你试剂盒的说明书对样品进行脱胶和DNA提取。PCR后，评估样本中HPV的存在。使用SPSS version 16进行数据分析，采用描述性统计(频率分布表和图表)、卡方和逻辑回归。结果:77例病变中，滤泡性20例，棘瘤性4例，基底细胞性3例，成丝细胞性8例，单囊性17例，丛状25例。此外，5个样本(6.5)HPV阳性。1例滤泡型，2例单囊型，2例丛状。结论:考虑到所检查病变中HPV的低患病率，不能认为该病毒参与了病变的病因和发病机制。因此，为了获得更准确的结果，必须在更大的样本量上进行进一步的调查。
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مقدمه: ادنتوژنیک کراتوسیست(OKC) یک نوع کیست رشدی تکاملی است که دارای خصوصیات تهاجمی و میزان عود بالا میباشد. کیست دنتیژروس شایعترین کیست رشدی تکاملی فکین میباشد، که توانایی تبدیل به نئوپلاسم را دارد.P53 و MDM2 (Murine double minute2) در برخی از کیستها و تومورهای ادنتوژنیک افزایش بیان پیدا میکنند. هدف از این مطالعه بررسی بروز نشانگرهای MDM2 و P53 در ادونتوژنیک کراتوسیست و کیست دنتیژروس و بررسی ارتباط آنها با فعالیت تکثیری بود. مواد و روشها: در این مطالعه به روش مقطعی، بیان پروتئینهای P53 و MDM2در 15 نمونه OKCو 15 نمونه کیست دنتیژروس بررسی شد. نتایج بااستفاده از آزمون آماریt-student و Chi square تجزیه و تحلیل شد. سطح معنیداری آزمونها 05/0 در نظر گرفته شد. یافته ها:بروز P53 و MDM2در تمامی نمونههای ادنتوژنیک کراتوسیست و کیست دنتیژروس دیده شد. بروز هر دو مارکر در ادنتوژنیک کراتوسیست بیشتر از کیست دنتیژروسبود.و بروز در لایههای سوپرابازال بیشتر از لایه بازال بود (045/0=(P. تفاوت آماری معنیداری مابین P53 و MDM2در کیست دنتیژروس وجود داشت (032/0=P)، ولی این اختلاف در OKCمعنیدار نبود (83/0=(P . نتیجه گیری: در این مطالعه میزان بروز مارکر MDM2 و P53 در ادنتوژنیک کراتوسیست از کیست دنتیژروس بیشتر بود. بر اساس نتایج بدست آمده میتوان چنین نتیجه گیری کرد که افزایش بیان P53 و MDM2در ادنتوژنیک کراتوسیست و کیست دنتیژروس ممکن است با پاتوژنز OKC و کیست دنتیژروس در ارتباط باشد و میتواند نشان دهنده رفتار بیولوژیک این دو کیست باشد.
{"title":"بررسی بروز MDM2 و P53 در کیست دنتیژروس و ادنتوژنیک کراتوکیست به روش ایمنوهیستوشیمی","authors":"ستاره شجاعی, شکوفه جمشیدی, نوشین محتشم, قدرت اله روشنایی, مهدیه شهابی نژاد","doi":"10.22038/jmds.2015.4471","DOIUrl":"https://doi.org/10.22038/jmds.2015.4471","url":null,"abstract":"مقدمه: ادنتوژنیک کراتوسیست(OKC) یک نوع کیست رشدی تکاملی است که دارای خصوصیات تهاجمی و میزان عود بالا میباشد. کیست دنتیژروس شایعترین کیست رشدی تکاملی فکین میباشد، که توانایی تبدیل به نئوپلاسم را دارد.P53 و MDM2 (Murine double minute2) در برخی از کیستها و تومورهای ادنتوژنیک افزایش بیان پیدا میکنند. هدف از این مطالعه بررسی بروز نشانگرهای MDM2 و P53 در ادونتوژنیک کراتوسیست و کیست دنتیژروس و بررسی ارتباط آنها با فعالیت تکثیری بود. مواد و روشها: در این مطالعه به روش مقطعی، بیان پروتئینهای P53 و MDM2در 15 نمونه OKCو 15 نمونه کیست دنتیژروس بررسی شد. نتایج بااستفاده از آزمون آماریt-student و Chi square تجزیه و تحلیل شد. سطح معنیداری آزمونها 05/0 در نظر گرفته شد. یافته ها:بروز P53 و MDM2در تمامی نمونههای ادنتوژنیک کراتوسیست و کیست دنتیژروس دیده شد. بروز هر دو مارکر در ادنتوژنیک کراتوسیست بیشتر از کیست دنتیژروسبود.و بروز در لایههای سوپرابازال بیشتر از لایه بازال بود (045/0=(P. تفاوت آماری معنیداری مابین P53 و MDM2در کیست دنتیژروس وجود داشت (032/0=P)، ولی این اختلاف در OKCمعنیدار نبود (83/0=(P . نتیجه گیری: در این مطالعه میزان بروز مارکر MDM2 و P53 در ادنتوژنیک کراتوسیست از کیست دنتیژروس بیشتر بود. بر اساس نتایج بدست آمده میتوان چنین نتیجه گیری کرد که افزایش بیان P53 و MDM2در ادنتوژنیک کراتوسیست و کیست دنتیژروس ممکن است با پاتوژنز OKC و کیست دنتیژروس در ارتباط باشد و میتواند نشان دهنده رفتار بیولوژیک این دو کیست باشد.","PeriodicalId":30335,"journal":{"name":"Journal of Mashhad Dental School","volume":"39 1","pages":"163-172"},"PeriodicalIF":0.0,"publicationDate":"2015-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68367472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
مقدمه: نشان داده شده است که دهانشویههای حاوی نانوذرات فلزی میتوانند به صورتی موثر در کنترل پلاک دندانی عمل نمایند. هدف از این تحقیق بررسی ثبات رنگ یک نوع ماده کامپوزیت بعد از مجاورت با چند محلول کلوئیدال حاوی ذرات نانو بود. مواد و روشها: ابتدا نمونههای کامپوزیتی تهیه و به مدت 24 ساعت در آب مقطر نگهداری شدند. سپس رنگ تمام نمونهها بر اساس سیستم CIELAB تعیین گردید (مرحله اول؛ ابتدای کار). در مرحله بعد، نمونهها به مدت 24 ساعت در دهانشویههای حاوی نانوذرات اکسیدتیتانیوم، اکسید روی، نقره، اکسید مس، کلرهگزیدین (کنترل مثبت) یا آب مقطر (کنترل منفی) غوطهور شدند و مجددا سنجش رنگ انجام شد (مرحله دوم؛ بـعد از قرار دادن در دهـانشویه). سپس سـطح نمونهها تـوسط مسواک بـرقی به مدت 1 دقیقه تمیز شد و نـمونهها برای سـومین بار رنگسنجی شدند (مرحله سوم؛ پس از مسواک زدن). میزان تغییر رنگ در میان گروههای مختلف با استفاده از آزمون واریانس یک عاملی (ANOVA) و پس آزمون توکی مقایسه گردید. (05/0=α) یافته ها: آزمون ANOVA تفاوت معنیداری را در تغییر رنگ بین مراحل مختلف درمان در میان گروههای مختلف نشان داد (05/0P<). تغییر رنگ بین مرحله اول و دوم در دهانشویه های نقره و کلرهگزیدین به صورت قابل توجهی کمتر از دهانشویههای اکسید مس و اکسید تیتانیوم و در تمام گروهها به صورت قابل توجهی بیشتر از آب مقطر بود (05/0P<). تغییر رنگ بین مراحل اول و سوم در تمام گروههای دهانشویه از لحاظ آماری در یک سطح و به صورت قابل توجهی بیشتر از گروه آب مقطر بود (05/0P<). نتیجه گیری: دهانشویههای حاوی نانو ذرات فلزی تغییر رنگی در حد کلرهگزیدین و بیشتر نشان دادند. مسواک زدن تأثیر اندکی در برداشتن رنگ از روی نمونههای کامپوزیت داشت.
{"title":"تاثیر دهانشویه های حاوری نانوذرات فلزی بر ثبات رنگ کامپوزیت رزین","authors":"فرزانه احراری, ندا اسلامی, رویا زمانی","doi":"10.22038/JMDS.2014.3601","DOIUrl":"https://doi.org/10.22038/JMDS.2014.3601","url":null,"abstract":"مقدمه: نشان داده شده است که دهانشویههای حاوی نانوذرات فلزی میتوانند به صورتی موثر در کنترل پلاک دندانی عمل نمایند. هدف از این تحقیق بررسی ثبات رنگ یک نوع ماده کامپوزیت بعد از مجاورت با چند محلول کلوئیدال حاوی ذرات نانو بود. مواد و روشها: ابتدا نمونههای کامپوزیتی تهیه و به مدت 24 ساعت در آب مقطر نگهداری شدند. سپس رنگ تمام نمونهها بر اساس سیستم CIELAB تعیین گردید (مرحله اول؛ ابتدای کار). در مرحله بعد، نمونهها به مدت 24 ساعت در دهانشویههای حاوی نانوذرات اکسیدتیتانیوم، اکسید روی، نقره، اکسید مس، کلرهگزیدین (کنترل مثبت) یا آب مقطر (کنترل منفی) غوطهور شدند و مجددا سنجش رنگ انجام شد (مرحله دوم؛ بـعد از قرار دادن در دهـانشویه). سپس سـطح نمونهها تـوسط مسواک بـرقی به مدت 1 دقیقه تمیز شد و نـمونهها برای سـومین بار رنگسنجی شدند (مرحله سوم؛ پس از مسواک زدن). میزان تغییر رنگ در میان گروههای مختلف با استفاده از آزمون واریانس یک عاملی (ANOVA) و پس آزمون توکی مقایسه گردید. (05/0=α) یافته ها: آزمون ANOVA تفاوت معنیداری را در تغییر رنگ بین مراحل مختلف درمان در میان گروههای مختلف نشان داد (05/0P<). تغییر رنگ بین مرحله اول و دوم در دهانشویه های نقره و کلرهگزیدین به صورت قابل توجهی کمتر از دهانشویههای اکسید مس و اکسید تیتانیوم و در تمام گروهها به صورت قابل توجهی بیشتر از آب مقطر بود (05/0P<). تغییر رنگ بین مراحل اول و سوم در تمام گروههای دهانشویه از لحاظ آماری در یک سطح و به صورت قابل توجهی بیشتر از گروه آب مقطر بود (05/0P<). نتیجه گیری: دهانشویههای حاوی نانو ذرات فلزی تغییر رنگی در حد کلرهگزیدین و بیشتر نشان دادند. مسواک زدن تأثیر اندکی در برداشتن رنگ از روی نمونههای کامپوزیت داشت.","PeriodicalId":30335,"journal":{"name":"Journal of Mashhad Dental School","volume":"39 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2015-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68367261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
مقدمه: چای سبز با اجزای شیمیایی فعال خود دارای خواص دارویی متنوعی از جمله آنتیاکسیدان، ضدالتهاب و ضدباکتری میباشد. اثرات بالقوه حفاظتی چای سبز بر سلامت دهان میتواند به دلیل وجود کاتچین و فلوراید موجود در آن وابسته می باشد. هدف از این مطالعه مرور نظام مند تحقیقات در دسترس برای ارزیابی اثر محصولات چای سبز بر کنترل و کاهش ژنژیویت ناشی از پلاک و پوسیدگی دندانی میباشد. مواد و روشها: این مطالعه در قالب مرورنظام مند مطالعات کارآزمایی بالینی تصادفی موجود در پایگاههای دادهای PubMed، Iranmedex، GoogleScholar و Cochrane بود. مقالات بر اساس چک لیستهای ارزیابی نقادانه بررسی شده و انتخاب شدند. پایاننامههای عمومی و تخصصی دندانپزشکی و داروسازی نیز بررسی گردیدند. شرکتکنندگان افراد سالم بدون بیماری سیستمیک و شدید پریودونتال بودند و مداخلات مصرف چای سبز به شکل دهانشویه، مصرف روزانه چای سبز، آدامس و به صورت مصرف موضعی بود. پیامدهای کاهش پلاک،پوسیدگی، خونریزی هنگام پروب کردن، عمق پاکت و از دست رفتن چسبندگی، شمارش استرپتوکوک موتانس و لاکتوباسیل و تغییر ترکیبات بزاق بررسی شد. یافتهها: در نتایج جستوجو، از 94 مقاله PubMed، 112 مقاله Google، 124 مقاله Iranmedex بعد از بررسی نقادانه تعداد مقالات انتخابی به 8 عدد کاهش یافت. نتایج مطالعات در قالب دو گروه پوسیدگی و بیماریهای پریودونتال تقسیم بندی و ارائه شدند. نتیجه گیری: چای سبز میتواند تأثیر مثبتی در سلامت دهان از طریق کاهش بروز پوسیدگی و بیماری پریودونتال داشته باشد. معیارهای بکار رفته در مطالعه بیماریهای پریودونتال از لحاظ بالینی باارزش تر بود و بررسی تأثیر چای سبز بر پوسیدگی دندانی نیازمند مطالعات طولانیتر میباشد.
{"title":"تاثیر چای سبز در کنترل و کاهش بیماریهای پریودنتال و پوسیدگی دندان - یک مرور نظام مند","authors":"بهاره طحانی, احسان مستاجران, ریحانه فقیهیان, فاطمه توکل, آیلین احتشامی, ساناز ضیایی","doi":"10.22038/jmds.2014.2254","DOIUrl":"https://doi.org/10.22038/jmds.2014.2254","url":null,"abstract":"مقدمه: چای سبز با اجزای شیمیایی فعال خود دارای خواص دارویی متنوعی از جمله آنتیاکسیدان، ضدالتهاب و ضدباکتری میباشد. اثرات بالقوه حفاظتی چای سبز بر سلامت دهان میتواند به دلیل وجود کاتچین و فلوراید موجود در آن وابسته می باشد. هدف از این مطالعه مرور نظام مند تحقیقات در دسترس برای ارزیابی اثر محصولات چای سبز بر کنترل و کاهش ژنژیویت ناشی از پلاک و پوسیدگی دندانی میباشد. \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000 \u0000مواد و روشها: این مطالعه در قالب مرورنظام مند مطالعات کارآزمایی بالینی تصادفی موجود در پایگاههای دادهای PubMed، Iranmedex، GoogleScholar و Cochrane بود. مقالات بر اساس چک لیستهای ارزیابی نقادانه بررسی شده و انتخاب شدند. پایاننامههای عمومی و تخصصی دندانپزشکی و داروسازی نیز بررسی گردیدند. شرکتکنندگان افراد سالم بدون بیماری سیستمیک و شدید پریودونتال بودند و مداخلات مصرف چای سبز به شکل دهانشویه، مصرف روزانه چای سبز، آدامس و به صورت مصرف موضعی بود. پیامدهای کاهش پلاک،پوسیدگی، خونریزی هنگام پروب کردن، عمق پاکت و از دست رفتن چسبندگی، شمارش استرپتوکوک موتانس و لاکتوباسیل و تغییر ترکیبات بزاق بررسی شد. \u0000یافتهها: در نتایج جستوجو، از 94 مقاله PubMed، 112 مقاله Google، 124 مقاله Iranmedex بعد از بررسی نقادانه تعداد مقالات انتخابی به 8 عدد کاهش یافت. نتایج مطالعات در قالب دو گروه پوسیدگی و بیماریهای پریودونتال تقسیم بندی و ارائه شدند. \u0000نتیجه گیری: چای سبز میتواند تأثیر مثبتی در سلامت دهان از طریق کاهش بروز پوسیدگی و بیماری پریودونتال داشته باشد. معیارهای بکار رفته در مطالعه بیماریهای پریودونتال از لحاظ بالینی باارزش تر بود و بررسی تأثیر چای سبز بر پوسیدگی دندانی نیازمند مطالعات طولانیتر میباشد.","PeriodicalId":30335,"journal":{"name":"Journal of Mashhad Dental School","volume":"45 1","pages":"169-184"},"PeriodicalIF":0.0,"publicationDate":"2014-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68367163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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