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Assessment of the current hydrochemical state of some fishponds of Kyiv region 基辅地区一些鱼塘的水化学现状评估
Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.020
L. Dragan, N. Mikhailenko, T. Bersan
Purpose. To assess the quality of the hydrochemical regime of fishponds, the territory of which suffered massive missile strikes or was directly a combat zone or close to the front line. Methodology. Chemical parameters of water including the content of ammonium nitrogen, nitrites, nitrates, phosphates and total iron were measured by the photometric method, while chloride concentration of chlorides by the argentometric titration method. The hydrogen indicator (pH) was determined electrometrically, and sulfate content and mineralization by the gravimetric method. All the above-mentioned methods are generally accepted in the comprehensive assessment of the ecological state of fishponds. Findings. The total amount of mineral salts dissolved in water was studied and analyzed in laboratory conditions, namely: the concentrations of calcium, magnesium, chlorine, bicarbonate ions and sulfate ions. The determined permanganate oxidizability in the investigated ponds exceeded the normative values by 72, 49, 31, and 42%, respectively. The concentration of nitrates ranged from 19.96 to 6.34 mg N/dm3, nitrites from 0.33 to 0.11 mgN/dm3, which indicates the intensity of decomposition of organic substances and indicates a certain degree of contamination of ponds. The amount of total iron in the studied ponds ranged from 1.98 to 1.17 mgFe/dm3, respectively. A slight increase in mineral phosphorus in ponds No. 1 and No. 2 was established by 31 and 26%, respectively, which can probably be related to both the decomposition of zooplankton and the input of pollutants from the outside from the surrounding areas. The obtained results of the laboratory analysis of the chemical composition of water samples as well as their comparison with the normative values for water bodies used for aquaculture provide a basis for assessing the current hydrochemical state of the investigated ponds of Kyiv region Originality. For the first time, a hydrochemical analysis of fishponds in Kyiv Region was conducted, the territory of which suffered massive missile strikes or was directly a combat zone or close to the front line. Practical value. The obtained results will contribute to a better understanding of the current ecological state of fishponds in Kyiv region. Keywords: water body, chemical analysis of water, toxic substances, ecological condition.
目的。评估鱼塘水化学状况的质量,这些鱼塘的领土遭受大规模导弹袭击,或直接是战区或靠近前线。方法。用光度法测定了水中铵态氮、亚硝酸盐、硝酸盐、磷酸盐和总铁的含量,用银量滴定法测定了水中氯化物的浓度。用电滴定法测定氢指示剂(pH),用重量法测定硫酸盐含量和矿化度。上述方法是鱼塘生态状况综合评价中普遍采用的方法。发现。在实验室条件下,研究和分析了溶解在水中的无机盐的总量,即:钙、镁、氯、碳酸氢盐离子和硫酸盐离子的浓度。调查池塘的高锰酸盐氧化性测定值分别超出标准值72、49、31和42%。硝酸盐的浓度在19.96 ~ 6.34 mgN/dm3之间,亚硝酸盐的浓度在0.33 ~ 0.11 mgN/dm3之间,说明了水体有机物分解的强度,表明水体受到了一定程度的污染。研究池塘的总铁含量分别为1.98 ~ 1.17 mgFe/dm3。1号池和2号池矿质磷略有增加,分别增加了31%和26%,这可能与浮游动物的分解和周围外来污染物的输入有关。对水样化学成分的实验室分析结果及其与水产养殖水体的规范值进行比较,为评估基辅地区原生态调查池塘的水化学现状提供了依据。首次对基辅地区的鱼塘进行了水化学分析,该地区遭受了大规模导弹袭击,或直接成为战区或靠近前线。实用价值。获得的结果将有助于更好地了解基辅地区鱼塘的当前生态状况。关键词:水体;水体化学分析;有毒物质;
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引用次数: 0
Black carp (Mylopharyngodon piceus Richardson, 1846). Thematic bibliography 黑鱼(Mylopharyngodon piceus Richardson, 1846)。专题书目
Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.157
Ir. Hrytsynyak, T. Shvets
Purpose. Forming a thematic bibliographic list of English-language publications on molecular-genetic, immunological, physiological-biochemical, as well as ecological and individual biological features of black carp (Mylopharyngodon piceus L.). Methods. The complete and selective methods were applied in the process of the systematic search. The bibliographic core have been consisted of English-language scientific publications from resources available to users of the Institute of Fisheries NAAS Scientific Library. Results. There was composed the thematic list of publications with a total quantity of 89 sources covering the time interval from 1995 to 2023, and highlighting the biological and ecological characteristics of a representative of the Cyprinids, the black carp, as well as considering the issue of its physiological, biochemical, genetic, and immunological features. The literary sources are arranged in alphabetical order by author or title, and described according to DSTU 8302:2015 “Information and documentation. Bibliographic reference. General principles and rules of composition”, with the amendments (code UKND 01.140.40), as well as in accordance with the requirements of APA style — international standard of references. Practical value. The list may be useful for scientists, practitioners, students, whose area of interests includes issues of biological research of Cyprinids, in particular black carp (Mylopharyngodon piceus L.). Key words: black carp, immunology, genetic indicators, biochemical indicators.
目的。形成关于黑鱼(Mylopharyngodon piceus L.)的分子遗传学、免疫学、生理生化、生态学和个体生物学特征的英文专题文献目录。方法。在系统搜索的过程中,采用了完整和选择性的方法。书目核心由渔业研究所科学图书馆用户可获得的资源中的英文科学出版物组成。结果。已编制了一份专题出版物清单,共有89个来源,涵盖1995年至2023年的时间间隔,突出了塞浦路斯的一种代表——黑鲤鱼的生物学和生态学特征,并考虑了其生理、生化、遗传和免疫学特征问题。文献来源按作者或标题按字母顺序排列,并根据DSTU 8302:2015“信息和文档”进行描述。文献参考。《构成的一般原则和规则》,及其修订(代码UKND 01.140.40),并符合APA风格的要求-国际参考标准。实用价值。该清单可能对科学家、从业者和学生有用,他们的兴趣领域包括鲤类生物研究问题,特别是黑鱼(Mylopharyngodon piceus L.)。关键词:青鱼;免疫学;遗传指标;
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引用次数: 0
Wintering of the eastern subtropical freshwater shrimp (Macrobrachium nipponense de Haan, 1849) in the conditions of the Dnister River basin 东亚热带淡水对虾(Macrobrachium nipponense de Haan, 1849)在德尼斯特河流域条件下的越冬
Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.003
P. Shekk, Yu. Astafurov
Purpose. To study the characteristics of wintering of Macrobrachium nipponense in natural conditions of the Lower Dniester basin and experimental assessment of the effect of physical condition, soil composition and type of shelter on the survival of wintering shrimps. Methodology. The results of field and experimental studies conducted in 2018–2020 were used in the study. The locations and conditions of mass wintering of shrimps in natural conditions in the lower reaches of the Dniester River were studied. The abundance of wintering individuals was determined by the method of direct counting and their physical condition was analyzed. Shrimps were kept in a RAS at the Laboratory of Aquatic Bioresources and Aquaculture of Odesa State Ecological University, where environmental parameters were maintained as close as possible to the conditions of wintering under natural conditions. In chronic experiments, the selectivity and effect of the composition of natural substrates and the thickness of the soil bed on shrimp survival were investigated. The selection and effectiveness of the shrimp’s use of above-ground shelters of various natural and anthropogenic origin for shrimp wintering was assessed depending on their physical condition (presence or absence of injuries). Shrimp for experiments were caught in October - November in the lower reaches of the Dniester River and the Dniester estuary. The location of mass wintering of shrimps was recorded using a GPS navigator. Findings. It was established that shrimp M. nipponense in natural conditions of the Lower Dniester river winters in aboveground shelters (of natural and anthropogenic origin) or by burrowing into the soil. The shrimp’s choice of aboveground shelters for wintering in natural conditions depended on their physical condition. Injured individuals (lost claws and limbs) preferred easily accessible aboveground shelters (sheaves of grass, flooded snags). Healthy individuals wintered in cracks between plant roots and stones. A significant part of shrimps overwintered in aboveground shelters of anthropogenic origin. The most complex and reliable aboveground shelters of natural origin were occupied by the least damaged shrimp individuals. More than 59% of shrimps are buried in the soil (soil shelters) for wintering. Healthy individuals prefer sandy soils or soils that contain sand. The highest yield of healthy shrimp overwintering was observed on sandy or sandy-gravel soils with a thicker soil layer. The depth of burial of shrimp depended on soil composition. It was the maximum in soils consisting of sand, sand-gravel mixture and chernozem or chernozem with inclusions of gravel. The survival of wintering individuals was inversely dependent on the depth of burial. The maximum yield of uninjured individuals from wintering was observed when they were buried in the soil, the minimum — when using aboveground shelters. The share of surviving injured shrimp in soil shelters decreased in proportion to the se
目的。研究日本沼虾(Macrobrachium nipponense)在Lower Dniester流域自然条件下的越冬特性,并实验评价物理条件、土壤组成和防护林类型对越冬虾存活的影响。方法。本研究采用了2018-2020年进行的实地研究和实验研究的结果。对德涅斯特河下游自然条件下虾群越冬的地点和条件进行了研究。用直接计数法测定了越冬个体的丰度,并对越冬个体的生理状况进行了分析。对虾饲养在敖德萨国立生态大学水生生物资源与水产养殖实验室的RAS中,环境参数尽可能接近自然条件下的越冬条件。在慢性试验中,研究了天然基质组成和土壤厚度对对虾生存的选择性和影响。根据虾的身体状况(有无受伤),评估了虾使用各种自然和人为来源的地上庇护所过冬的选择和有效性。用于实验的虾于10 - 11月在德涅斯特河下游和德涅斯特河口捕获。使用GPS导航仪记录了虾群越冬的地点。发现。在德涅斯特河下游的自然条件下,日本虾在地上(自然的和人为的)的庇护所中过冬,或者在土壤中挖洞。虾在自然条件下选择地上的避难所过冬取决于它们的身体状况。受伤的个体(失去了爪子和四肢)更喜欢容易接近的地上庇护所(草堆,被水淹没的树枝)。健康的个体在植物根和石头之间的裂缝中过冬。相当一部分虾在人为的地上庇护所越冬。最复杂和最可靠的自然来源的地上庇护所是由受害最小的虾个体占据的。超过59%的虾被埋在土壤(土壤掩体)中过冬。健康的人更喜欢沙质土壤或含沙的土壤。在土层较厚的砂质或砂砾质土壤上,健康虾越冬产量最高。虾的埋藏深度与土壤成分有关。在由砂土、砂砾混合土和黑钙土或含砾石包裹体的黑钙土中,其含量最高。越冬个体的存活率与埋藏深度成反比。未受伤个体越冬产量最高的是埋在土壤中,最低的是使用地上掩体。在土壤掩体中幸存的受伤虾的比例与受伤的严重程度成正比。受伤个体的最大生存是通过在地上的避难所过冬来保证的。创意。本文首次报道了在德涅斯特河下游条件下外来对虾日本沼虾越冬的野外观测资料。自然条件下大规模越冬的地点和条件已经确定。作为实验研究的结果,分析了虾在越冬期间对各种自然和人为来源的土壤和地上庇护所的选择性和有效性,它们的选择性取决于越冬个体的身体状况(有无伤害)。实用价值。在气候渐进式变化的条件下,迫切的任务之一是使有前途的水产养殖对象适应新的自然生态系统。所得结果可用于乌克兰南部水产养殖中日本沼虾的人为繁殖和控制养殖方法的发展。关键词:德涅斯特河,日本沼虾,越冬,地上庇护所,地上庇护所,个体身体状况,生存
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引用次数: 0
Modern aspects of pikeperch (Sander lucioperca Linnaeus, 1758) aquaculture (a review) 鲈的现代方面(Sander lucioperca Linnaeus, 1758)水产养殖(综述)
Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.134
O. Polishchuk, M. Simon
Purpose. To analyze data from special literature on the current state and basic methods of pikeperch (Sander lucioperca Linnaeus, 1758) aquaculture in Ukraine and other European countries. To determine the differences in technologies for growing this fish species depending on the specifics of the resource provision of aquaculture and regional pecularities of the functioning of fish farming enterprises in European countries. Findings. The review examines the general principles of aquaculture of pikeperch according to various technological schemes, including in the conditions of ponds and industrial-type farms. The peculiarities of the cultivation of different age groups of this fish species in the specific conditions of the development of aquaculture in Ukraine have been clarified. Some peculiarities of the application of treatment and preventive measures used in the process of its cultivation are described. The problems faced by Ukrainian producers of fish products during the organization of feeding of this percid fish are emphasized. Data from domestic and foreign information sources on productivity, methods of wintering and transportation of this member of the European ichthyofauna are summarized. The main prospects for the further development of pikeperch aquaculture in Ukraine and related problems, in particular in the field of technological support, have been determined. Practical Value. The data presented in the review can be used by practitioners of the fish industry and scientists in the field of fisheries in the process of developing pikeperch aquaculture in Ukraine. This will contribute to the improvement of the regulatory and technological base of pond fish farming and increase the efficiency of the development of industrial methods of aquaculture in the farms of different regions of the country. At the same time, the information presented in the review can be used for the training of fisheries specialists during the development of relevant training programs of educational institutions. Keywords: pikepersh(Sander lucioperca Linnaeus, 1758), biocontrol effect, pond aquaculture, industrial aquaculture, recirculation aquaculture system (RAS), reservoirs.
目的。分析乌克兰和欧洲其他国家梭鲈养殖现状和基本方法的专题文献资料。根据水产养殖资源提供的具体情况和欧洲国家养鱼企业运作的区域特点,确定养殖这种鱼类的技术差异。发现。根据各种技术方案,包括在池塘和工业化农场的条件下,审查了鲈鱼养殖的一般原则。在乌克兰发展水产养殖的具体条件下,已经澄清了这种鱼类不同年龄组的养殖特点。介绍了其栽培过程中处理和预防措施的应用特点。强调了乌克兰鱼产品生产者在组织喂养这种鱼类时所面临的问题。本文总结了国内外有关该鱼类的生产力、越冬和运输方法的资料。已经确定了乌克兰鲈鱼养殖进一步发展的主要前景和有关问题,特别是技术支助领域的问题。实用价值。审查中提供的数据可供渔业从业者和渔业领域的科学家在乌克兰发展梭鲈水产养殖的过程中使用。这将有助于改善池塘养鱼的管理和技术基础,并提高该国不同地区养殖场发展工业化水产养殖方法的效率。同时,审查中提供的信息可用于教育机构在制定相关培训方案期间对渔业专家的培训。关键词:梭鲈(Sander lucioperca Linnaeus, 1758),生物防治效果,池塘养殖,工业养殖,循环水养殖系统,水库
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引用次数: 0
Evaluation of the genetic variability of pedigree stocks of amur carp (Cyprinus rubrofuscus Lacépède, 1803) 黑龙江鲤鱼(Cyprinus rubrofuscus lac, 1803)系谱种群遗传变异性的评价
Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.086
А. Mariutsa, I. Hrystyniak, Yu. Glushko, Т. Nahorniuk
Purpose. To study the genetic peculiarities of pedigree tocksof Amur carp and evaluate its genetic variability by analyzing distribution of alleles and genotypes by the specific protein systems and cytogenetic parameters. Methodology. Analysis of polymorphism of protein systems was performed using electrophoresis in polyacrylamide gel. As protein markers to evaluate the genetic structure of Amur carp stocks, the separation of allelic and genotypic frequencies by loci, which encode a number of fish blood proteins, were used: transferrin (TF), albumin (ALB) and esterase (EST, 3.1.1.1). Cytogenetic analysis was performed using the micronucleus test and analysis of apoptosisfrequencies. Collection and processing of biological material of fish as well as statistical processing of the obtained data was performed using generally accepted methods. Findings. A comprehensive analysis of the genetic structure of Amur carp from three farms in Ukraine was carried out by protein systems and cytogenetic markers. The results of the study showed that all pedigree groups of Amur carp were characterized by a deviation towards the predominance of heterozygotes (Fis = from –0.084 to –0.344). A minimal imbalance was observed in the group of carp from the farm “Karpatskyi Vodogray” LLC (Fis = –0.084). The maximum violation of the genetic balance was found in carp from the farm of JSC “Sumyrybhosp” (Fis = –0.308) and in the group from the experimental farm “Veliky Lyubin” (Fis = –0.344). In general, the analysis of the protein systems of fish blood showed a high level of heterogeneity of pedigree stocks of Amur carp from different farms in Ukraine. The analysis of frequencies of cytogenetic parameters of Amur carp from three fish farms showed that fish from fish farms of JSC “Sumyrybhosp” and PFE “Dzherelo” were characterized by a lower frequency of erythrocytes with micronuclei (EMN) (3.3±0.3 ‰), (3.2±0.3 ‰), lymphocytes with micronuclei (LMN) (2.1±0, 2 ‰), (1.9±0.2 ‰), and apoptosis (4.2±0.3‰), (4,3±0,3‰) compared to the group from fish farm “Karpatskyi Vodogray” LLC, where these values were as follows: EMN (4.7±0.3 ‰), LMN (2.4±0.2‰), apoptosis (5.6±0.4‰). These results indicates a lower level of destabilization of chromosomal apparatus of Amur carp from the fish farm JSC “Sumyrybhosp” at the time of the study. Originality. A comprehensive evaluation of the level of variability of the genetic structure of pedigree stocks of Amur carp from different regions of Ukraine was performed for the first time. Practical Value. Pedigree socksof Amur carp have important practical value in selective breeding work with carps, including works for obtaining hybrid lines to increase the resistance of breeding material. Keywords: Amur carp, genetic structure, locus, alleles, genotype, heterozygosity, micronucleus test, cytogenetic analysis.
目的。目的:研究黑龙江鲢鱼家系的遗传特性,分析其等位基因和基因型的分布,并根据特定的蛋白质系统和细胞遗传学参数评价其遗传变异性。方法。用聚丙烯酰胺凝胶电泳分析了蛋白质体系的多态性。作为评价黑龙江鲤种群遗传结构的蛋白质标记,采用基因座对转铁蛋白(TF)、白蛋白(ALB)和酯酶(EST, 3.1.1.1)等鱼类血液蛋白的等位基因频率和基因型频率进行分离。细胞遗传学分析采用微核试验和凋亡频率分析。鱼类生物材料的收集和处理以及所得数据的统计处理采用普遍接受的方法进行。发现。采用蛋白质系统和细胞遗传标记对乌克兰三个养殖场的黑龙江鲤的遗传结构进行了全面分析。结果表明:黑龙江鲤各家系群体杂合子优势度存在一定偏差(Fis = -0.084 ~ -0.344)。在“Karpatskyi Vodogray”LLC养殖场的鲤鱼组中观察到最小的不平衡(Fis = -0.084)。“Sumyrybhosp”鱼场和“Veliky Lyubin”实验鱼场的遗传平衡破坏最大(Fis = -0.308)。总体而言,对鱼血蛋白质系统的分析表明,乌克兰不同养殖场的黑龙江鲤系谱种群存在高度异质性。对3个渔场黑龙江鲤细胞遗传学参数的频率分析表明,与Karpatskyi Vodogray LLC鱼场相比,JSC“Sumyrybhosp”和PFE“Dzherelo”鱼场的鱼类微核红细胞(EMN)(3.3±0.3‰)、微核淋巴细胞(LMN)(2.1±0.2‰)、(1.9±0.2‰)和细胞凋亡(4.2±0.3‰)、(4.3±0.3‰)的频率较低,其数值如下:人物(4.7±0.3‰),LMN(2.4±0.2‰),细胞凋亡(5.6±0.4‰)。这些结果表明,在研究期间,来自JSC“Sumyrybhosp”渔场的黑龙江鲤鱼染色体装置的不稳定程度较低。创意。首次对乌克兰不同地区黑龙江鲤家系种群遗传结构变异水平进行了综合评价。实用价值。纯种黑龙江鲤在选育工作中具有重要的实用价值,包括选育杂交种以提高选育材料的抗性。关键词:黑龙江鲤,遗传结构,基因座,等位基因,基因型,杂合性,微核试验,细胞遗传学分析
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引用次数: 0
Biological peculiarities of artificial reproduction of sturgeons (Acipenseriformes) (a rеview) 中华鲟人工繁殖的生物学特性(概览)
Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.057
O. Kuzmenko, N. Vovk
Purpose. To analyze the scientific data of domestic and foreign authors regarding the peculiarities of artificial reproduction of sturgeonss (Acipenseriformes). To investigate the state of study of the phenomenon of polyspermic fertilization in sturgeons and the factors that induce it in the conditions of artificial reproduction and cultivation. Findings. A review analysis of the results of scientific studies by domestic and foreign authors was carried out on the pecularities of artificial reproduction of sturgeons and the phenomenon of polysperm fertilization of their eggs in controlled conditions of aquaculture. It is shown that information on the problems of sturgeon polyspermy and its induction by various factors was practically absent in the available professional literature in recent years. Since during the artificial reproduction of sturgeons, a significant number of embryos have atypical development, which leads to their death even before hatching, it has been suggested that atypical forms of cell division were caused by polyspermy. The conducted studies added to the knowledge about the peculiarities of the structure of the sturgeon egg envelope and the processes that occur during fertilization. Despite the fact that there is a natural mechanism to prevent the possibility of polyspermic fertilization, this phenomenon occurs quite often, causing an impairment of zygote fragmentation at the initial stages of embryogenesis, and therefore a decrease in the yield of larvae. Understaning of the mechanism of blocking polyspermy in sturgeons and the factors that induce it requires further studies. The list of scientific articles includes 61 sources, 58 in English. The published publications mainly cover the period of the last twenty years. Practical value. A review of data from scientific and professional sources on the specified topic will allow a comprehensive approach to the understanding of the peculiarities of artificial reproduction of sturgeons, the phenomenon of polyspermy and increasing the yield of larvae in sturgeon farming. Review material on this topic can be interesting and useful for scientists, practitioners, students whose interests are related to aquaculture and used in the educational process of training specialists in specialty 207 “Aquatic bioresources and aquaculture”. Keywords: aquaculture, sturgeons (Acipen­seriformes), eggs, egg envelope, micropyle, fertilization, cortical reaction, polyspermy, embryos.
目的。分析国内外作者关于鲟鱼人工繁殖特点的科学资料。探讨在人工繁殖和养殖条件下鲟鱼多精受精现象及其诱发因素的研究现状。发现。综述了国内外有关中华鲟人工繁殖的特点及在控制养殖条件下中华鲟卵多精受精现象的研究成果。结果表明,近年来有关鲟鱼多精问题及其各种因素诱导的信息在现有的专业文献中几乎是缺失的。由于在鲟鱼的人工繁殖过程中,有相当数量的胚胎具有非典型发育,甚至在孵化之前就导致它们死亡,因此有人认为非典型形式的细胞分裂是由多精子引起的。所进行的研究增加了关于鲟鱼卵包膜结构的特殊性和受精过程的知识。尽管存在一种自然机制来阻止多精受精的可能性,但这种现象经常发生,导致胚胎发生初期受精卵破碎受损,从而导致幼虫产量下降。对鲟鱼阻断多精的机制和诱导多精的因素的了解还需要进一步的研究。科学文章列表包括61个来源,其中58个是英文的。出版的出版物主要涵盖近二十年的时间。实用价值。对来自科学和专业来源的有关特定主题的数据进行审查,将有助于全面了解鲟鱼人工繁殖的特点、多精现象和鲟鱼养殖中增加幼虫产量的问题。对于对水产养殖感兴趣的科学家、从业者和学生来说,这一主题的复习材料可能是有趣和有用的,并用于培训专业207“水生生物资源和水产养殖”专家的教育过程中。关键词:水产养殖,中华鲟,卵,卵包膜,微孔,受精,皮质反应,多精,胚胎。
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引用次数: 0
Perspectives of artificial reproduction of the vyrezub (Rutilus frisii frisii Nordman, 1840) in Ukraine (a rеview) 乌克兰vyrezub (Rutilus frisii frisii Nordman, 1840)人工繁殖的展望(全景图)
Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.034
V. Gurbyk, A. Mruk, A. Kucheruk
Purpose. The main goal of the thematic review was the analysis and generalization of literary sources on the peculiarities of its biology, technological aspects of artificial reproduction and growing of vyrezub (Rutilus frisii frisii Nordman, 1840) for the restoration of its natural population in water bodies of Ukraine. To evaluate the perspectives of artificial reproduction and growing of vyrezub in aquaculture of Ukraine. Findings. As a result of human impact on river networks of Ukraine, the abundance of vyrezub significantly decreased, it has become a rare and at the same time an endangered species. According to retrospective data, vyrezub previously belonged to the widespread commercial species of the Dnipro and Southern Bug rivers. The biological features of this species have been analyzed, the observance of which can allow obtaining viable fish seeds in aquaculture conditions. The adaptive possibilities of vyrezub to artificial conditions of reproduction have been reviewed. Historical information on the reproduction of this species over the last century has been analyzed. On the territory of Ukraine, first attempts to artificially reproduce vyrezub were carried out by specialists of Ukrcherrybvod. According to the results of studies of Ukrainian scientists, several stages of artificial reproduction of vyrezub have been identified, namely: taking spawners from natural water bodies, obtaining eggs and sperm, incubation of eggs in incubation devices, obtaining 6 g fish seeds, release of viable juveniles into natural water bodies. However, the works on artificial reproduction of vyrezub were not successful, which was the reason for their complete cessation. In particular, this species is promising for growing in polyculture, as it does not create intense trophic competition with most objects of freshwater aquaculture, and as a result of the consumption of mollusks will reduce the likelihood of the spread of some invasive fish diseases. Practical value. The review of information sources on various aspects of the use of vyrezub in aquaculture will contribute to the development of a system of separate links of the technology of its cultivation for the needs of rearing in the conditions of aquaculture enterprises and stocking of internal water bodies with viable juveniles. Key words: vyrezub (Rutilus frisii frisii Nord­man, 1840), aquaculture, biocontrol effect, artificial reproduction, viable juveniles, stocking, restoration of population abundance.
目的。专题审查的主要目标是对其生物学特性、人工繁殖和vyrezub (Rutilus frisii frisii Nordman, 1840年)生长的技术方面的文献资料进行分析和概括,以恢复其在乌克兰水体中的自然种群。目的:评价乌克兰水产业中维祖鱼的人工繁殖和生长前景。发现。由于人类对乌克兰河流网络的影响,vyrezub的丰度大大减少,它已成为一种罕见的同时也是一种濒危物种。根据回顾性数据,vyrezub以前属于第聂伯罗河和南布格河的广泛商业物种。对该物种的生物学特性进行了分析,遵守这些特性可以在水产养殖条件下获得活鱼种子。综述了兔对人工繁殖条件的适应可能性。对上个世纪该物种繁殖的历史信息进行了分析。在乌克兰领土上,乌克兰的专家首次尝试人工繁殖vyrezub。根据乌克兰科学家的研究结果,已经确定了vyrezub人工繁殖的几个阶段,即:从自然水体中获取产卵,获得卵子和精子,在孵化装置中孵育卵子,获得6克鱼籽,将可存活的幼鱼释放到自然水体中。然而,对vyrezub的人工繁殖工作并不成功,这是他们完全停止的原因。特别是,该物种很有希望在混养中生长,因为它不会与大多数淡水水产养殖对象产生激烈的营养竞争,并且由于食用软体动物将减少一些侵入性鱼类疾病传播的可能性。实用价值。审查关于在水产养殖中使用维雷祖鱼的各个方面的资料来源,将有助于发展一个培育维雷祖鱼技术的单独联系系统,以满足在水产养殖企业条件下饲养和在内部水体中放养可存活的幼鱼的需要。关键词:紫尾螺(Rutilus frisii frisii Nord-man, 1840),水产养殖,生物防治效果,人工繁殖,活苗,放养,种群丰度恢复
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引用次数: 0
Peculiarities of ligulosis of bream (Abramis brama Linnaeus, 1758) and roach (Rutilus rutilus Linnaeus, 1758) in the Dnipro (Zaporizhzhiа) reservoir 第聂伯罗水库鲷鱼(Abramis brama Linnaeus, 1758)和蟑螂(Rutilus Rutilus Linnaeus, 1758)舌病的特点
Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.119
V. Sydorenko, O. Marenkov
Purpose. Study of the peculiarities of ligula infestation of roach and bream in the Dnipro (Zaporizhzhia) reservoir and the influence of Ligula intestinalis on the morphological and cytometric parameters of infected fish species. Methodology. The work presents the generalized results of studies conducted in 2020-2021 during planned test catches. Sampling was carried out along the shoreline of the Dnieper (Zaporizhzhia) reservoir in the lower part of the villages of Viiskove and Mykilske-na-Dnipri, Dnipropetrovsk region, in the summer-autumn period. The morphological study was carried out by carefully examining the surface of the scales and skin cover. Special attention was paid on fins, the amount of mucus, its consistency, color change, the presence of tumors, hemorrhages, ulcers, cysts, large ectoparasites, and the condition of eyes. The parasitological study was carried out by a complete anatomical dissection of the fish according to the classical method. Blood was collected at the catch site from the heart of the fish using a needle and a syringe into a dry test tube for the biochemical serum analysis. Blood was also collected for the preparation of smears and their subsequent microscopy. Blood smears were fixed with methyl alcohol and stained according to Romanovsky’s method with Giemsa’s standard solution. Blood smears were examined by microscopy at a 40x magnification. Blood smears of non-infected fish were used for comparison. Thirty fields of view were examined in each smear. Findings. In the course of the study, a decrease in the weight and condition factor of infected roach and bream was observed, and irreversible pathological changes in the internal organs caused by the mechanical pressure and migration of parasites were recorded. In addition, deviations from the normal values were found in the formed elements of blood. About 24.3% of erythrocytes in infected specimens of bream and roach were deformed: the cell membrane was not clear, erythrocytes had a slightly inflated shape. In some erythrocytes, the nucleus was faintly visible. Infected specimens had more juvenile forms of erythrocytes compared to non-infected fish. Originality. For the first time, a comparative morphological, cytometric analysis of blood during ligulosis infestation of infected specimens of bream and roach in the Dnipro (Zaporizhzhia) reservoir is presented. Practical value. The study of biological, clinical and epizootic peculiarities of parasitic helminths is of urgent interest for both science and fish farming. The obtained results can be implemented in the practical activity of fish farming and used in the development of measures to limit and prevent the development of parasitic diseases among the ichthyofauna of the Dnipro (Zaporizhzhia) reservoir. Key words: bream, roach, ligula, erythrocytes, albumins.
目的。第聂伯罗水库蟑螂和鲷鱼舌虫侵染特点及肠舌虫对侵染鱼类形态和细胞参数影响的研究。方法。这项工作介绍了2020-2021年计划测试捕捞期间进行的研究的总体结果。夏秋季节,在第聂伯罗彼得罗夫斯克地区Viiskove村和Mykilske-na-Dnipri村下游沿第聂伯河水库岸线取样。形态学研究是通过仔细检查鳞片表面和皮肤覆盖物进行的。特别注意的是鳍、粘液的数量、粘稠度、颜色变化、肿瘤、出血、溃疡、囊肿、大的体外寄生虫的存在以及眼睛的状况。寄生虫学研究是根据经典方法对鱼进行完整的解剖。在捕获部位用针和注射器从鱼的心脏采集血液,放入干试管中进行生化血清分析。还采集血液用于涂片的制备和随后的显微镜检查。血液涂片用甲醇固定,用吉姆萨标准溶液按罗曼诺夫斯基法染色。用40倍放大镜检查血液涂片。未感染鱼的血涂片用于比较。每个涂片检查30个视野。发现。在研究过程中,观察到感染的蟑螂和鲷鱼的体重和条件因子下降,并记录了由寄生虫的机械压力和迁移引起的内脏不可逆的病理变化。此外,在血液的形成元素中发现与正常值的偏差。感染的鲷鱼和蟑螂标本中约有24.3%的红细胞变形:细胞膜不清,红细胞略有膨大。在一些红细胞中,细胞核隐约可见。与未感染的鱼相比,感染的鱼有更多的幼红细胞。创意。首次对第聂伯罗水库感染的鲷鱼和蟑螂进行了口舌病侵染期间的血液形态、细胞分析比较。实用价值。寄生蠕虫的生物学、临床和兽疫学特性的研究对科学和养鱼都具有迫切的意义。所得结果可应用于实际养鱼活动,并可用于制定限制和预防第聂伯罗水库鱼系动物寄生虫病发生的措施。关键词:鲤鱼,蟑螂,舌鱼,红细胞,白蛋白。
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引用次数: 0
Histological structure of gills of Prussian carp of the Samara River of the Dnipropetrovsk region 第聂伯罗彼得罗夫斯克地区萨马拉河普鲁士鲤鱼鳃的组织学结构
Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.102
K. Mashkova, T. Sharamok
Purpose. To study the histological structure of the gill apparatus of the Prussian carp (Сarassius gibelio) from the point of view of the presence of pathologies with differentiation according to areas with different levels of anthropogenic pressure of the Samara River in the Dnipropetrovsk region. Methodology. The generalized sutdy results were obtained during 2019–2021. The study was conducted within three sampling sites with different types of human impact. The object of the study were age-6 Prussian carps of both sexes. Histological studies were performed in accordance with generally accepted methods. Gills were obtained by anatomical dissection from freshly caught fish. Photographs of histological specimens were taken using a digital camera “SciencelabT5005.17M” and an microscope “Ulab XY-B2TLED”. Measurements of the samples were performed using the “ScienceLabView7”. Statistical processing of the obtained data was carried out in MS Excel 2010. Findings. The conducted histological studies showed that pathological changes in the structure of the gill apparatus were observed in age-6 Prussian carps in all sampling sites of the Samara River. In particular, such pathologies as curvature of lamellae terminals, accumulation of mucus and hyperplasia of the epithelium of gill filaments, fusion of lamellae were found. Single cases of necrosis and twisting of lamellae threads were recorded. Most often, histopathological changes in the gill apparatus were observed in fish caught near the city Novomoskovsk. Organometric studies showed that there was no significant difference between the length and width of gill arches of Prussian carp from different sampling sites. The length and width of the lamellae of the gill apparatus was the largest in individuals sampled near the city Novomoskovsk, which may indicate a greater need for oxygen for the fish body in the specified area. The length of the respiratory lamellae was the lowest in Prissian carp sampled near the village of Novoselivka. The lowest width of the lamellae were found in fish from the village of Khashcheve. The obtained data give reason to conclude that the conditions of existence for Prussian carp are most favorable near the village of Novoselivka. Originality. For the first time the histological structure and pathology of the gills aparatus of Prussian carp in the conditions of the Samara River in the Dnipropetrovsk region was studied. Practical value. The obtained data on the histological changes of the gill apparatus of fish can be used to identify adaptive reactions, will allow assessing the impact of anthropogenic factors on hydrobionts, and will also provide the opportunity to monitor the morpho-physiological state of a specific population under the impact of pollutants of various origins. Key words: Prussian carp, gill apparatus, morphological and histological indicators.
目的。根据第聂伯罗彼得罗夫斯克地区萨马拉河不同的人为压力水平,从存在不同病理的角度研究普鲁士鲤鱼(Сarassius gibelio)鳃器的组织学结构。方法。广义研究结果在2019-2021年期间获得。这项研究是在三个有不同类型人类影响的采样地点进行的。这项研究的对象是6岁的普鲁士鲤鱼,男女都有。组织学研究按照普遍接受的方法进行。从新鲜捕获的鱼解剖得到鳃。使用数码相机“SciencelabT5005.17M”和显微镜“Ulab XY-B2TLED”拍摄组织学标本。使用“ScienceLabView7”对样品进行测量。所得数据在MS Excel 2010中进行统计处理。发现。组织学研究表明,在萨马拉河的所有采样点,6岁普鲁士鲤鱼的鳃器结构都发生了病理变化。尤其是鳃片末端弯曲、鳃丝上皮粘液堆积增生、鳃片融合等病变。记录了单例片层线坏死和扭转。最常见的是,在新莫斯科夫斯克市附近捕获的鱼中观察到鳃器官的组织病理学变化。器官计量学研究表明,不同取样地点的普鲁士鲤鳃弓的长度和宽度没有显著差异。在Novomoskovsk市附近取样的个体中,鳃器片的长度和宽度最大,这可能表明该特定区域的鱼体对氧气的需求更大。在novvoselivka村附近取样的百里鱼呼吸片层长度最短。最小宽度的薄片是在哈什乔夫村的鱼身上发现的。所得的资料使我们有理由得出结论,普鲁士鲤鱼的生存条件在诺沃塞利夫卡村附近是最有利的。创意。首次研究了第聂伯罗彼得罗夫斯克地区萨马拉河条件下普鲁士鲤鱼鳃部的组织学结构和病理学。实用价值。所获得的关于鱼鳃器官组织学变化的数据可用于确定适应性反应,将允许评估人为因素对水生生物的影响,并将提供机会监测在各种来源的污染物影响下特定种群的形态生理状态。关键词:鲤鱼,鳃器,形态学和组织学指标。
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引用次数: 0
Efficiency of use of prebiotics based on manane oligosaccharides in carp feeding 甘露寡糖益生元在鲤鱼饲料中的利用效率
Pub Date : 2021-09-30 DOI: 10.15407/fsu2021.03.060
О. Dobryanska
Purpose. Analysis of productive and economic indicators in the cultivation of age-1+ carp as a result of the use of prebiotics based on mannan oligosaccharides (MOS) in feeding. Methodology. Four ponds were used, two of which were experimental and two control ponds, which were stocked with age-1 scaly carp with an average weight of 76–77 g at a stocking density of 2200 specimens/ha. Fish of Experiment 1 and Control 1 were fed with extruded feed with a protein content of 23%, fish in Experiment 2 and Control 2 were fed with a grain mixture with a protein content of 19%. 0.05% prebiotic based on MOS "Actigen" was added to the diet of experimental groups of carp throughout the growing season. Optimal conditions for carp farming were ensured including control over oxygen, hydrochemical and hydrobiological regimes in ponds, which were measured according to the generally accepted methods in fish farming. Fishery and economic indicators of growing age-1+ were determined after harvesting fish in autumn. Findings. Major hydrochemical parameters in all ponds were within the normative values. The average seasonal biomass of forage organisms of zooplankton and zoobenthos during the growing season did not differ in the experimental ponds and amounted to 10.33-16.23 g/m3 and 1.23-2.96 g/m3. A positive effect of the use of prebiotics based on MOS in the feeding of age-1+ carp was established. When the prebiotic was given as a part of the extruded feed, the average weight of fish was higher by 11.5%, and fish productivity by 22.7% relative to the control group, while the cost of feed for cultivation was lower by 20%. When it was added to the grain mixture, these values were higher by 12.9%, 16.3% and 16.7%, respectively. The profit, as a result of the use of the prebiotic based on MOS in the composition of the extruded feed increased by 43.3% compared to Control 1, and by 19.1% compared to Control 2 when it was added to the grain mixture. Originality. For the first time, a comparative analysis of the effectiveness of the use of the prebiotic based on MOS in the composition of extruded feed and grain mixtures was performed taking into account productivity and economic indicators. Practical value. The obtained results indicate the feasibility and effectiveness of the use of prebiotics based on MOS in the feeding of age-1+ carp. Introduction of experimental additives to feeds of different composition helps to obtain additional growth and increase the economic component in the cultivation of carp to marketable condition. Key words: carp, prebiotic, feed, productivity, efficiency, profit hydrochemical and hydrobiological indicators.
目的。甘露寡糖(MOS)益生元对1+龄鲤鱼养殖的生产经济指标分析方法。采用4个鱼塘,其中2个为试验鱼塘,2个为对照鱼塘,放养平均体重76 ~ 77 g的1龄鳞鱼,放养密度为2200条/ha。试验1和对照1分别饲喂蛋白质含量为23%的挤压饲料,试验2和对照2分别饲喂蛋白质含量为19%的杂粮混合饲料。在整个生长季节,在试验组鲤鱼饲料中添加0.05%的MOS“Actigen”益生元。确保鲤鱼养殖的最佳条件,包括控制池塘中的氧气、水化学和水生物制度,这些都是根据养鱼中普遍接受的方法进行测量的。秋季采鱼后测定生长期1+的渔业经济指标。发现。各水塘主要水化学参数均在规范范围内。实验池浮游动物和底栖动物生长季饲料生物的季节平均生物量无显著差异,分别为10.33 ~ 16.23 g/m3和1.23 ~ 2.96 g/m3。以MOS为基础的益生元在1岁以上鲤鱼的饲养中具有良好的效果。在挤压饲料中添加益生元,鱼的平均体重比对照组高11.5%,鱼的产量比对照组高22.7%,养殖饲料成本比对照组低20%。当添加到谷物混合物中时,这些值分别提高了12.9%,16.3%和16.7%。在挤压饲料中添加以MOS为基础的益生元,与对照1相比,利润增加了43.3%;与对照2相比,将其添加到谷物混合物中,利润增加了19.1%。创意。本文首次结合生产力和经济指标,对基于MOS的益生元在膨化饲料和谷物混合物中使用的有效性进行了比较分析。实用价值。上述结果表明,基于MOS的益生元在1岁以上鲤鱼饲料中应用的可行性和有效性。在不同组成的饲料中添加试验性添加剂有助于获得额外的生长,并使鲤鱼养殖的经济成分达到可销售的条件。关键词:鲤鱼,益生元,饲料,生产力,效率,利润水化学和水生物指标。
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Ribogospodars''ka Nauka Ukraini
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