Pub Date : 2015-11-01DOI: 10.5455/SPATULA.20151118060743
Winisia E. Makirita, L. Chauka, M. Chacha
{"title":"Larvicidal activity of Clausena anisata fruits and leaves extracts against Anopheles gambiae Giles s.s, Culex quinquefasciatus Say and Aedes egyptiae -","authors":"Winisia E. Makirita, L. Chauka, M. Chacha","doi":"10.5455/SPATULA.20151118060743","DOIUrl":"https://doi.org/10.5455/SPATULA.20151118060743","url":null,"abstract":"","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"39 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131210993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-08-01DOI: 10.5455/SPATULA.20130806054707
M. G. Nyamoita, Z. Mbwambo, B. J. Ochola, E. Innocent, W. Lwande, A. Hassanali
AIM: The aim of this study was to evaluate the acetone and methanol root bark extracts of Vitex payos and different fractions thereof, following acetone extraction and column chromatographic separation, for their immediate toxicity and long term effects on An. gambiae Giles sensu stricto larvae under semi-field conditions. MATERIALS & METHOD: Third and early fourth instars of An. gambiae larvae were exposed to acetone and methanol extracts of V. payos root bark and the acetone chromatographic fractions. Their effects on larval, pupal and adult stages were recorded. Consequently, GC/MS analysis on the extracts was carried out. RESULTS: Larvicidal effects of the extracts and fractions were found to be largely associated with phytoecdysteroids and saponins which tested positive in the test materials. The fractions were more potent than the crude extracts. Long-term (11-14 days) post-exposure observations at lower doses showed that the extracts achieved over 90% adult growth inhibition with morphogenetic variations and behavioral changes. CONCLUSION: Extracts and chromatographic fractions from V. payos possess larvicidal and/or IGR principles which can be used in the local communities where most of the people cannot afford synthetic larvicides.
{"title":"Chemical composition and evaluation of mosquito larvicidal activity of Vitex payos extracts against Anopheles gambiae Giles S.S larvae","authors":"M. G. Nyamoita, Z. Mbwambo, B. J. Ochola, E. Innocent, W. Lwande, A. Hassanali","doi":"10.5455/SPATULA.20130806054707","DOIUrl":"https://doi.org/10.5455/SPATULA.20130806054707","url":null,"abstract":"AIM: The aim of this study was to evaluate the acetone and methanol root bark extracts of Vitex payos and different fractions thereof, following acetone extraction and column chromatographic separation, for their immediate toxicity and long term effects on An. gambiae Giles sensu stricto larvae under semi-field conditions. \u0000MATERIALS & METHOD: Third and early fourth instars of An. gambiae larvae were exposed to acetone and methanol extracts of V. payos root bark and the acetone chromatographic fractions. Their effects on larval, pupal and adult stages were recorded. Consequently, GC/MS analysis on the extracts was carried out. \u0000RESULTS: Larvicidal effects of the extracts and fractions were found to be largely associated with phytoecdysteroids and saponins which tested positive in the test materials. The fractions were more potent than the crude extracts. Long-term (11-14 days) post-exposure observations at lower doses showed that the extracts achieved over 90% adult growth inhibition with morphogenetic variations and behavioral changes. \u0000CONCLUSION: Extracts and chromatographic fractions from V. payos possess larvicidal and/or IGR principles which can be used in the local communities where most of the people cannot afford synthetic larvicides.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131888312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/SPATULA.20160314094649
M. Afyon, C. Artuk
Hepatitis B virus (HBV) therapy is still a topic of discussion. Because of that approved agents for HBV have some disadvantages, there is a need for new treatment targets and approaches for HBV. New treatment targets for HBV consist revers transcription, immunoregulation, cccDNA formation and regulation, maturation and secretion of infectious virions and subviral particules, viral receptor, entry and cytosolic transport, nucleocapsid assembly. Therapeutic vaccination and traditional Chinese medicine are the other options for novel agents for HBV treatment.
{"title":"New drug targets and new approaches for Hepatitis B virus theraphy","authors":"M. Afyon, C. Artuk","doi":"10.5455/SPATULA.20160314094649","DOIUrl":"https://doi.org/10.5455/SPATULA.20160314094649","url":null,"abstract":"Hepatitis B virus (HBV) therapy is still a topic of discussion. Because of that approved agents for HBV have some disadvantages, there is a need for new treatment targets and approaches for HBV. New treatment targets for HBV consist revers transcription, immunoregulation, cccDNA formation and regulation, maturation and secretion of infectious virions and subviral particules, viral receptor, entry and cytosolic transport, nucleocapsid assembly. Therapeutic vaccination and traditional Chinese medicine are the other options for novel agents for HBV treatment.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"11 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121000405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/SPATULA.20101209111244
Özden Dedeli, A. Karadakovan
Aim: Aim of this study was conducted to determine to use medicine and complemantary and alternative medicine practices which are used for these diseases, problems experienced during medicine use and elderly people living at their own home enviroment. Methods: The population of this descriptive study was all the Izmir/Nebahat Dolman Yasli Dayanisma Merkezi. Besides, sampling method was not be used. 103 elderly people was inclued study who was suitable include criteria. The necessary permissions for conducting the study were taken. Questinnaire form composed of relavent literature, was filled with face to face interview. Study procedures were reviewed and approved by the Ethics Committee. Data were evaluated on computer by using SPSS v 11.0 software programme, mean, percentage analysis. Results: The mean age of study population was 71.7±5.3 and most of this elderly were female (75.7%). 93.2% of elderly had a chronic disease and they used three and/or over drugs (51.5%). In addition elderly used their drugs, they used herbs (62.1%) and complementary alternative therapy (54.3%). As results of research most of elderly prefer to use herbs mix and complementary alternative therapy, owing to be healthy/wellness. Conclusion: We suggest, the future research should be regarding whether benefit or not harmful in older person, who was used both the drugs were ordered by doctor and/or non-ordered and herbs mix and complementary alternative therapy. Whole health professional and elderly should be given, through the agency of comprehensive research.
{"title":"Investigation of Complementary and Alternative Medicine Practice and Drug Use in the Elderly","authors":"Özden Dedeli, A. Karadakovan","doi":"10.5455/SPATULA.20101209111244","DOIUrl":"https://doi.org/10.5455/SPATULA.20101209111244","url":null,"abstract":"Aim: Aim of this study was conducted to determine to use medicine and complemantary and alternative medicine practices which are used for these diseases, problems experienced during medicine use and elderly people living at their own home enviroment. \u0000Methods: The population of this descriptive study was all the Izmir/Nebahat Dolman Yasli Dayanisma Merkezi. Besides, sampling method was not be used. 103 elderly people was inclued study who was suitable include criteria. The necessary permissions for conducting the study were taken. Questinnaire form composed of relavent literature, was filled with face to face interview. Study procedures were reviewed and approved by the Ethics Committee. Data were evaluated on computer by using SPSS v 11.0 software programme, mean, percentage analysis. \u0000Results: The mean age of study population was 71.7±5.3 and most of this elderly were female (75.7%). 93.2% of elderly had a chronic disease and they used three and/or over drugs (51.5%). In addition elderly used their drugs, they used herbs (62.1%) and complementary alternative therapy (54.3%). As results of research most of elderly prefer to use herbs mix and complementary alternative therapy, owing to be healthy/wellness. \u0000Conclusion: We suggest, the future research should be regarding whether benefit or not harmful in older person, who was used both the drugs were ordered by doctor and/or non-ordered and herbs mix and complementary alternative therapy. Whole health professional and elderly should be given, through the agency of comprehensive research.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"2 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127357876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/SPATULA.20130218124721
T. Taşkın, L. Bitiş
SUMMARY AIM: The aim of this study is to evaluate for the first time the antioxidant activities of two wild edible plants ( Silene alba subsp. divaricata leaves and Stellaria media subsp. media aerial parts),which were collected in both fresh edible as vegetable and flowering (unedible) periods and to determine their relationship with the phenolic content. METHODS: The antioxidant capacities of the extracts were evaluated by different methods, DPPH radical-scavenging, ABTS radical cation scavenging and metal chelating activity assays, including total phenolic contents. RESULTS: Silene alba subsp. divaricata extract, which was collected in edible period contained the highest phenolic compounds. This plant, which was collected in both periods showed higher DPPH radical-scavenging and ABTS radical cation scavenging activity than Stellaria media subsp. media while Stellaria media subsp. media extract, which was collected in edible period had the highest metal chelating activity. The present study reports for the first time the antioxidant activity of Silene alba subsp. divaricata .
{"title":"Antioxidant activity of Silene alba subsp. divaricata and Stellaria media subsp. media from Caryophyllaceae","authors":"T. Taşkın, L. Bitiş","doi":"10.5455/SPATULA.20130218124721","DOIUrl":"https://doi.org/10.5455/SPATULA.20130218124721","url":null,"abstract":"SUMMARY AIM: The aim of this study is to evaluate for the first time the antioxidant activities of two wild edible plants ( Silene alba subsp. divaricata leaves and Stellaria media subsp. media aerial parts),which were collected in both fresh edible as vegetable and flowering (unedible) periods and to determine their relationship with the phenolic content. METHODS: The antioxidant capacities of the extracts were evaluated by different methods, DPPH radical-scavenging, ABTS radical cation scavenging and metal chelating activity assays, including total phenolic contents. RESULTS: Silene alba subsp. divaricata extract, which was collected in edible period contained the highest phenolic compounds. This plant, which was collected in both periods showed higher DPPH radical-scavenging and ABTS radical cation scavenging activity than Stellaria media subsp. media while Stellaria media subsp. media extract, which was collected in edible period had the highest metal chelating activity. The present study reports for the first time the antioxidant activity of Silene alba subsp. divaricata .","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126130539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/SPATULA.20121112072137
S. Khosla, Sunny Sharma
Botanicals have been used traditionally by herbalists and indigenous healers worldwide for the prevention and treatment of many diseases. Basic scientific research has uncovered the mechanisms by which some plants afford their therapeutic effects. Emblica officinalis (Amla) is major tree in Indian system of Medicine. Emblica officinalis (Amla) are widely used in the ayurvedic medicines and believed to increase defense against diseases. Emblica officinalis due to their strong antioxidant and biological properties prevent innumerable health disorders related to oxidative stress, cardiovascular diseases, neurodegenerative diseases and cancer. It also contains essential nutrients. This article discus important pharmacogenetic property of Emblica officinalis. In this communication, we reviewed the applications of Emblica officinalis for the treatment of various other diseases.
{"title":"A short description on pharmacogenetic properties of Emblica officinalis","authors":"S. Khosla, Sunny Sharma","doi":"10.5455/SPATULA.20121112072137","DOIUrl":"https://doi.org/10.5455/SPATULA.20121112072137","url":null,"abstract":"Botanicals have been used traditionally by herbalists and indigenous healers worldwide for the prevention and treatment of many diseases. Basic scientific research has uncovered the mechanisms by which some plants afford their therapeutic effects. Emblica officinalis (Amla) is major tree in Indian system of Medicine. Emblica officinalis (Amla) are widely used in the ayurvedic medicines and believed to increase defense against diseases. Emblica officinalis due to their strong antioxidant and biological properties prevent innumerable health disorders related to oxidative stress, cardiovascular diseases, neurodegenerative diseases and cancer. It also contains essential nutrients. This article discus important pharmacogenetic property of Emblica officinalis. In this communication, we reviewed the applications of Emblica officinalis for the treatment of various other diseases.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"1996 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123550781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/SPATULA.20111220112858
Sanjeev Rastogi
Ayurveda, a commonly practiced system of medicine in India has its unique proposition to the concept of health and disease. At one end it identifies various material based properties which determine the pharmacological property of the drug used; on the other this also identifies various the psycho- physical composition of person to be treated as a factor which may influence the action profile of the drug under use. Vatsanabha (Aconite) finds its place in Ayurvedic materia medica since antiquity though with a clear caution before it is used. A deadly material can also be turned into a saviour or even the vise-versa depending upon the methods of use,Ayurveda explains. Ayurveda proposes a traditional purification technique to reduce the harmful effects associated with Vatsanabha. A willful or ignorance of the principles of Ayurveda, from the stage of drug manufacturing to the stage of consumption is supposed to cause unpredictable drug responses which may result in adverse reactions as the one is reported in the review, or in under responses as is commonly accused. The review gives us opportunity to feel that a thoughtful understanding of Ayurvedic pharmacology, its clinical principles and limitations is required for its optimal utilization. We should therefore, be ready to get criticized for inconsistent theories if there are any, and should welcome with open heart for the principles which are proving their worth on scientific grounds.
{"title":"A review of Aconite (Vatsanabha) usage in Ayurvedic formulations: traditional views and their inferences -","authors":"Sanjeev Rastogi","doi":"10.5455/SPATULA.20111220112858","DOIUrl":"https://doi.org/10.5455/SPATULA.20111220112858","url":null,"abstract":"Ayurveda, a commonly practiced system of medicine in India has its unique proposition to the concept of health and disease. At one end it identifies various material based properties which determine the pharmacological property of the drug used; on the other this also identifies various the psycho- physical composition of person to be treated as a factor which may influence the action profile of the drug under use. \u0000Vatsanabha (Aconite) finds its place in Ayurvedic materia medica since antiquity though with a clear caution before it is used. A deadly material can also be turned into a saviour or even the vise-versa depending upon the methods of use,Ayurveda explains. Ayurveda proposes a traditional purification technique to reduce the harmful effects associated with Vatsanabha. A willful or ignorance of the principles of Ayurveda, from the stage of drug manufacturing to the stage of consumption is supposed to cause unpredictable drug responses which may result in adverse reactions as the one is reported in the review, or in under responses as is commonly accused. \u0000The review gives us opportunity to feel that a thoughtful understanding of Ayurvedic pharmacology, its clinical principles and limitations is required for its optimal utilization. We should therefore, be ready to get criticized for inconsistent theories if there are any, and should welcome with open heart for the principles which are proving their worth on scientific grounds.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125278686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/SPATULA.20120501080940
A. Raju, A. J. M. Christina, Anita Murali
ABSTRACT Objective: The objective of the present study was to evaluate antitumor potential of Drosera burmannii vahl by using Ehrlich's Ascites Carcinoma (EAC) tumor model in swiss albino mice. Materials and Method: The normal control, EAC control, standard (5FU) and the ethanol and aqueous extracts (250 and 500mg/kg each) of D. burmannii vahl + EAC (four groups) were given the respective treatments24 h after tumor cell inoculation,for14 days. After 14 days, the antitumor potential was evaluated by increase in body weight, % Increase in Life Span (%ILS), tumor cell count, Mean Survival Time (MST), Packed Cell Volume (PCV) and various hematological parameters. Once the animals were sacrificed, the vital organs were removed for histopathological and antioxidant activity studies. Result: Oral administration of crude extracts increased the %ILS, MST and decreased the peritoneal ascetic fluid content, body weight and PCV significantly (pEEDB250=AEDB500>AEDB 250 respectively.
{"title":"Antitumor activity of ethanol and aqueous extracts of Drosera burmannii vahl. in EAC bearing mice.","authors":"A. Raju, A. J. M. Christina, Anita Murali","doi":"10.5455/SPATULA.20120501080940","DOIUrl":"https://doi.org/10.5455/SPATULA.20120501080940","url":null,"abstract":"ABSTRACT \u0000Objective: The objective of the present study was to evaluate antitumor potential of Drosera burmannii vahl by using Ehrlich's Ascites Carcinoma (EAC) tumor model in swiss albino mice. \u0000 Materials and Method: The normal control, EAC control, standard (5FU) and the ethanol and aqueous extracts (250 and 500mg/kg each) of D. burmannii vahl + EAC (four groups) were given the respective treatments24 h after tumor cell inoculation,for14 days. After 14 days, the antitumor potential was evaluated by increase in body weight, % Increase in Life Span (%ILS), tumor cell count, Mean Survival Time (MST), Packed Cell Volume (PCV) and various hematological parameters. Once the animals were sacrificed, the vital organs were removed for histopathological and antioxidant activity studies. \u0000Result: Oral administration of crude extracts increased the %ILS, MST and decreased the peritoneal ascetic fluid content, body weight and PCV significantly (pEEDB250=AEDB500>AEDB 250 respectively.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125525278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/SPATULA.20140625114124
E. Erdogan, A. Everest, M. Serin
Aim: Salvia heldreichiana Boiss.ex Benth. which belongs to Lamiaceae family is an endemic species from Mersin region, in Turkey. It has been utilised the some extracts and essential oil of the plant. Especially, the essential oil of Salvia heldreichiana known as antimicrobial, antioxidant and anticancer agent is commonly used in alternative medicine. However, using it without controlling its reliability has made investigation of mutagenic potential of the essential oil necessary. Methods: In the present study, the mutagenicity of the essential oil was researched by Ames-test which is a short-term bacterial test. The essential oil of plant was extracted with hydrodistillation method. The experiments were performed in two groups as both with S9 metabolic and without it by using Salmonella typhimurium strains TA98 and TA100. Results: The test results were evaluated according to number of revertant colony compared with negative controls. In summary, it was seen that the essential oil of Salvia heldreichiana does not have any mutagenic activity both at the 1- 0,03 mg/ml concantration intervals and with S9 and S9-free environment . It wasn’t found that the amount of revertant colony does not considerably differ from the amount of spontaneous revertant colony for both TA98 and TA100 strains.
{"title":"The Investigation of Mutagenic Effect of Salvia heldreichiana Essential Oil by Ames test","authors":"E. Erdogan, A. Everest, M. Serin","doi":"10.5455/SPATULA.20140625114124","DOIUrl":"https://doi.org/10.5455/SPATULA.20140625114124","url":null,"abstract":"Aim: Salvia heldreichiana Boiss.ex Benth. which belongs to Lamiaceae family is an endemic species from Mersin region, in Turkey. It has been utilised the some extracts and essential oil of the plant. Especially, the essential oil of Salvia heldreichiana known as antimicrobial, antioxidant and anticancer agent is commonly used in alternative medicine. However, using it without controlling its reliability has made investigation of mutagenic potential of the essential oil necessary. \u0000Methods: In the present study, the mutagenicity of the essential oil was researched by Ames-test which is a short-term bacterial test. The essential oil of plant was extracted with hydrodistillation method. The experiments were performed in two groups as both with S9 metabolic and without it by using Salmonella typhimurium strains TA98 and TA100. \u0000Results: The test results were evaluated according to number of revertant colony compared with negative controls. In summary, it was seen that the essential oil of Salvia heldreichiana does not have any mutagenic activity both at the 1- 0,03 mg/ml concantration intervals and with S9 and S9-free environment . \u0000It wasn’t found that the amount of revertant colony does not considerably differ from the amount of spontaneous revertant colony for both TA98 and TA100 strains.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126634549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.5455/spatula.20151117065119
R. Ghosh, Anita Murali, S. Ramu
Aim: To evaluate the diuretic efficacy of alcohol extract of Mitragyna parvifolia root (a substitute of Kadamba) and determine its anti-urolithiatic activity in Wistar rats. Methods: Alcohol extract of M. parvifolia root was evaluated for diuretic activity at doses 200 and 400 mg/kg. Parameters such as volume, pH, sodium, potassium, chloride and creatinine content of urine were measured. Urolithiasis was induced in rats by administering 1% v/v ethylene glycol in drinking water for 28 d with ammonium chloride 1% w/v for the first 3 d. The alcohol extract at doses 200 and 400 mg/kg were administered from the 15th day onwards as curative regimen. Parameters evaluated were pH and content of oxalate, calcium, magnesium, phosphate and uric acid in urine. Serum parameters blood urea nitrogen, calcium and creatinine were also measured. GSH, MDA, SOD and catalase were estimated in kidney homogenates. Histopathological examination of the kidneys was also performed. Results: The alcohol extract of M. parvifolia root failed to show significant diuretic activity. However the extract significantly (P < 0.001) normalized the elevated levels of urine and serum parameters in lithiatic rats. There was a significant improvement in antioxidant status of the kidney as well. Conclusion: Results of this study indicate that M. parvifolia root is useful for the treatment of urinary calculi.
{"title":"Diuretic effects of Mitragyna parvifolia Korth. root extract and its therapeutic efficacy against experimental urolithiasis in rats","authors":"R. Ghosh, Anita Murali, S. Ramu","doi":"10.5455/spatula.20151117065119","DOIUrl":"https://doi.org/10.5455/spatula.20151117065119","url":null,"abstract":"Aim: To evaluate the diuretic efficacy of alcohol extract of Mitragyna parvifolia root (a substitute of Kadamba) and determine its anti-urolithiatic activity in Wistar rats. \u0000Methods: Alcohol extract of M. parvifolia root was evaluated for diuretic activity at doses 200 and 400 mg/kg. Parameters such as volume, pH, sodium, potassium, chloride and creatinine content of urine were measured. Urolithiasis was induced in rats by administering 1% v/v ethylene glycol in drinking water for 28 d with ammonium chloride 1% w/v for the first 3 d. The alcohol extract at doses 200 and 400 mg/kg were administered from the 15th day onwards as curative regimen. Parameters evaluated were pH and content of oxalate, calcium, magnesium, phosphate and uric acid in urine. Serum parameters blood urea nitrogen, calcium and creatinine were also measured. GSH, MDA, SOD and catalase were estimated in kidney homogenates. Histopathological examination of the kidneys was also performed. \u0000Results: The alcohol extract of M. parvifolia root failed to show significant diuretic activity. However the extract significantly (P < 0.001) normalized the elevated levels of urine and serum parameters in lithiatic rats. There was a significant improvement in antioxidant status of the kidney as well. \u0000Conclusion: Results of this study indicate that M. parvifolia root is useful for the treatment of urinary calculi.","PeriodicalId":309051,"journal":{"name":"Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126955747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：