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Rumen Fermentation of Local Grasses Feed to Native Goat 饲喂本地山羊的本地草的瘤胃发酵
Pub Date : 2024-02-07 DOI: 10.20956/hajas.v5i1.24777
Ismartoyo Ismartoyo, R. Islamiyati, Muhammad Rusdy
The aim of this research was to examine the feed rumen fermentation in the rumen of goat fed 4 different diets. This study was designed based on the Latin Square Design which consists of 4 diet treatments  with 4 replications in each diet treatment. A total of 4 male goats, with relatively the same weight and age, were randomly assigned to an individual metabolic cage fed with 4 diets. The four diets studied were R1: Elephant grass, R2: Mini elephant grass, R3: Panicum maximum grass and R4: Brachiaria decumbens grass. Each diet was added with 20% of rice bran. The result of this experiment indicated that there was no any significant effect of different diets on the pH values, concentrations of VFA (Volatile fatty acids), and Ammonia (NH3) in the rumen of goat. The pH values in the rumen of goat treated with R1, R2, R3, and R4 were 7.17, 7.12, 7.07, and 7.0, respectively. The concentration of ammonia (mM) in the rumen of goat treated with R1, R2, R3, and R4 were 4.6, 3.3. 4.5, and 4.1, respectively. Whereas the level of VFA (mM) in the rumen of goat treated with R1, R2, R3, and R4 were 40.4, 43.6, 48.7, and 49.9, respectively. There was no any significantly difference (p>0.05) in the characteristic of rumen fermentation between the diet treatment. This might be due to the similarity in the nutrient content of those grasses studied. The characteristic of rumen fermentation was in the range of optimal growth of rumen microbes. It is concluded that those of four grasses studied edible, acceptable and contain a good nutrient as a feed resource for goat. The nutrient content of the grasses studied creates a good rumen environment for optimum feed fermentation in the rumen of goat.  
本研究的目的是检测饲喂 4 种不同日粮的山羊瘤胃中的饲料瘤胃发酵情况。本研究采用拉丁方阵设计,包括 4 种日粮处理,每种日粮处理有 4 次重复。总共 4 只体重和年龄相对相同的雄性山羊被随机分配到一个饲喂 4 种日粮的代谢笼中。研究的四种日粮分别是 R1:大象草,R2:小象草、R3:R4: 十日草。每种日粮都添加了 20% 的米糠。实验结果表明,不同日粮对山羊瘤胃中的 pH 值、挥发性脂肪酸(VFA)浓度和氨氮(NH3)没有明显影响。用 R1、R2、R3 和 R4 处理山羊瘤胃的 pH 值分别为 7.17、7.12、7.07 和 7.0。用 R1、R2、R3 和 R4 处理的山羊瘤胃中的氨浓度(毫摩尔)分别为 4.6、3.3、4.5 和 4.1。4.5和4.1。而用 R1、R2、R3 和 R4 处理的山羊瘤胃中的 VFA 水平(毫摩尔)分别为 40.4、43.6、48.7 和 49.9。不同日粮处理的山羊瘤胃发酵特征没有明显差异(P>0.05)。这可能是由于这些草的营养成分相似。瘤胃发酵特性处于瘤胃微生物的最佳生长范围。结论是,所研究的四种草均可食用、可接受且含有良好的营养成分,可作为山羊的饲料资源。所研究牧草的营养成分为山羊瘤胃中的最佳饲料发酵创造了良好的瘤胃环境。
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引用次数: 0
Rumen Fermentation of Local Grasses Feed to Native Goat 饲喂本地山羊的本地草的瘤胃发酵
Pub Date : 2024-02-07 DOI: 10.20956/hajas.v5i1.24777
Ismartoyo Ismartoyo, R. Islamiyati, Muhammad Rusdy
The aim of this research was to examine the feed rumen fermentation in the rumen of goat fed 4 different diets. This study was designed based on the Latin Square Design which consists of 4 diet treatments  with 4 replications in each diet treatment. A total of 4 male goats, with relatively the same weight and age, were randomly assigned to an individual metabolic cage fed with 4 diets. The four diets studied were R1: Elephant grass, R2: Mini elephant grass, R3: Panicum maximum grass and R4: Brachiaria decumbens grass. Each diet was added with 20% of rice bran. The result of this experiment indicated that there was no any significant effect of different diets on the pH values, concentrations of VFA (Volatile fatty acids), and Ammonia (NH3) in the rumen of goat. The pH values in the rumen of goat treated with R1, R2, R3, and R4 were 7.17, 7.12, 7.07, and 7.0, respectively. The concentration of ammonia (mM) in the rumen of goat treated with R1, R2, R3, and R4 were 4.6, 3.3. 4.5, and 4.1, respectively. Whereas the level of VFA (mM) in the rumen of goat treated with R1, R2, R3, and R4 were 40.4, 43.6, 48.7, and 49.9, respectively. There was no any significantly difference (p>0.05) in the characteristic of rumen fermentation between the diet treatment. This might be due to the similarity in the nutrient content of those grasses studied. The characteristic of rumen fermentation was in the range of optimal growth of rumen microbes. It is concluded that those of four grasses studied edible, acceptable and contain a good nutrient as a feed resource for goat. The nutrient content of the grasses studied creates a good rumen environment for optimum feed fermentation in the rumen of goat.  
本研究的目的是检测饲喂 4 种不同日粮的山羊瘤胃中的饲料瘤胃发酵情况。本研究采用拉丁方阵设计,包括 4 种日粮处理,每种日粮处理有 4 次重复。总共 4 只体重和年龄相对相同的雄性山羊被随机分配到一个饲喂 4 种日粮的代谢笼中。研究的四种日粮分别是 R1:大象草,R2:小象草、R3:R4: 十日草。每种日粮都添加了 20% 的米糠。实验结果表明,不同日粮对山羊瘤胃中的 pH 值、挥发性脂肪酸(VFA)浓度和氨氮(NH3)没有明显影响。用 R1、R2、R3 和 R4 处理山羊瘤胃的 pH 值分别为 7.17、7.12、7.07 和 7.0。用 R1、R2、R3 和 R4 处理的山羊瘤胃中的氨浓度(毫摩尔)分别为 4.6、3.3、4.5 和 4.1。4.5和4.1。而用 R1、R2、R3 和 R4 处理的山羊瘤胃中的 VFA 水平(毫摩尔)分别为 40.4、43.6、48.7 和 49.9。不同日粮处理的山羊瘤胃发酵特征没有明显差异(P>0.05)。这可能是由于这些草的营养成分相似。瘤胃发酵特性处于瘤胃微生物的最佳生长范围。结论是,所研究的四种草均可食用、可接受且含有良好的营养成分,可作为山羊的饲料资源。所研究牧草的营养成分为山羊瘤胃中的最佳饲料发酵创造了良好的瘤胃环境。
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引用次数: 0
Level of DNA similarity the horned and polled Bali Cattle Using Microsatellite approach 利用微卫星方法研究巴里牛的DNA相似性
Pub Date : 2023-02-16 DOI: 10.20956/hajas.v4i2.21798
Zulkharnaim Maupa, S. Baco, L. Rahim, Muhammad Yusuf
The phenomenon of polled on Bali cattle should have a scientific explanation that explains the validity of the breed. Whether polled Bali cattle still have a genetic relationship with horned Bali cattle or are the results of crosses with other cattle breeds. This study used microsatellite markers to identified the genetic diversity and relationship between polled and horned in Bali cattle. The number of samples of 40 polled Bali cattle and 189 Bali horned cattle. The age of the study sample was 2.5 - 3.5 years. Research data analysis includes: genotype and allele frequencies, Hardy Weinberg Equilibrium analyze, observed and expected heterozygosity values, and polymorphic informative content. Polled Bali cattle have similar morphological and genetic characteristics with horned Bali cattle based on HEL9, INRA035, ILSTS045, and HEL13 microsatellite. HEL9, INRA035, ILSTS045, and HEL13 microsatellite can be a genetic marker for polled Bali cattle.
巴厘岛牛的投票现象应该有一个科学的解释,以解释该品种的有效性。被调查的巴厘牛是否仍然与有角的巴厘牛有遗传关系,还是与其他牛品种杂交的结果。本研究利用微卫星标记技术对巴厘牛的遗传多样性及其与有轮和有角牛的关系进行了研究。样本数量为40头巴厘牛和189头巴厘角牛。研究样本的年龄在2.5 - 3.5岁之间。研究数据分析包括:基因型和等位基因频率、Hardy Weinberg平衡分析、观察到的和预期的杂合度值、多态信息含量。基于HEL9、INRA035、ILSTS045和HEL13微卫星的检测结果显示,轮作巴厘牛的形态和遗传特征与有角巴厘牛相似。HEL9、INRA035、ILSTS045和HEL13微卫星可以作为巴厘牛的遗传标记。
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引用次数: 0
Complete Feed Based on Fermented Sago Waste Against the Consumption and Digestion of Goat 发酵西米废饲料对山羊消费消化的影响
Pub Date : 2023-01-31 DOI: 10.20956/hajas.v4i2.19278
Fadly Fadly, S. Syahrir, R. Islamiyati
A common method used to improve the nutritional quality of feed is by fermentation method.  The working principle is that fermentation activates the growth and metabolism of microorganism so that it can increase the digestibility of feed and produce the preferred aroma of livestock.  This study aims to find out the effect of giving fermented sago waste on the consumption and digestibility of goat and cattle. Two feed treatments were tested on 12 goats.  The results showed that the consumption and digestibility value of Dry Matter (DM) and Crude Protein (CP) showed no noticeable difference in goat cattle. The study concluded that complete feed-based fermented sago waste did not affect the consumption and digestibility of CP and DM of goat.Keywords: Sago waste, completed feed, consumption, digestion, dry matter, crude protein
提高饲料营养品质的常用方法是发酵法。其工作原理是通过发酵激活微生物的生长代谢,从而提高饲料的消化率,产生牲畜喜欢的香气。本试验旨在研究饲喂发酵西米粪对山羊和牛消耗和消化率的影响。对12只山羊进行了两种饲料处理试验。结果表明:羊牛对干物质(DM)和粗蛋白质(CP)的消耗和消化率无显著差异。由此得出,完全饲料发酵西米渣不影响山羊粗蛋白质和干物质的消耗和消化率。关键词:西米废料,完成饲料,消耗量,消化,干物质,粗蛋白质
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引用次数: 0
Progressive Motility, DNA Fragmentation, Intact Plasma Membrane, and Acrosome Status of Frozen Semen Bali and Simmental Bulls 巴厘公牛和西门塔尔公牛冷冻精液的渐进式运动、DNA断裂、完整质膜和顶体状态
Pub Date : 2023-01-22 DOI: 10.20956/hajas.v4i2.23351
Kirana Dara Dinanti Adiputra, Sukandi Sukandi, Siti Farida, H. Sonjaya, H. Hasbi
In increasing the population of bulls in Indonesia, it is necessary to utilize Artificial Insemination (AI) technology. AI reproductive technology aims to increase reproductive efficiency and use superior semen in livestock and can prevent the spread of reproductive diseases. Frozen semen is one of the factors that influence the success of AI. Thus, the quality of frozen semen must be maintained so fertility remains good. This study aimed to determine the quality of frozen semen of Bali and Simmental bulls at the Artificial Insemination Center, South Sulawesi. This study used frozen semen from two Bali and Simmental bulls. Parameters observed were progressive motility, deoxyribonucleic acid fragmentation (DNA), acrosome status, and intact plasma membrane. The progressive motility was tested using Computer-assisted sperm analysis (CASA Sperm Vision Minitube), the DNA fragmentation using acridine orange dye, the acrosome status using peanut agglutinin, and the intact plasma membrane staining using HOS solution. This study gives a result that the progressive motility, DNA fragmentation, and acrosome status of spermatozoa in frozen semen of Bali and Simmental bulls were not significantly different (P>0.05). In contrast, the whole plasma membrane of spermatozoa in Bali bulls' frozen semen was significantly higher (P<0.05) than in Simmental bulls. The results indicate that based on the parameters of progressive motility, DNA fragmentation, and acrosome status of spermatozoa, both bull breeds had the same quality of frozen semen. Meanwhile, according to the spermatozoa intact plasma membrane, the frozen semen of Bali bulls had better quality than that of Simmental bulls.Keywords: Artificial Insemination, frozen semen quality, Bali and Simmental bulls
为了增加印尼公牛的数量,有必要利用人工授精(AI)技术。人工智能生殖技术旨在提高牲畜的生殖效率和使用优质精液,并可以防止生殖疾病的传播。冷冻精液是影响人工智能成功的因素之一。因此,必须保持冷冻精液的质量,以保持良好的生育能力。本研究旨在确定南苏拉威西人工授精中心的巴厘公牛和西门塔尔公牛冷冻精液的质量。这项研究使用了两头巴厘公牛和西门塔尔公牛的冷冻精液。观察的参数包括进行性运动、脱氧核糖核酸片段(DNA)、顶体状态和完整的质膜。采用计算机辅助精子分析(CASA精子视觉迷你管)检测精子运动能力,吖啶橙染色检测DNA片段化,花生凝集素检测顶体状态,HOS溶液检测完整质膜。结果表明,巴厘公牛和西门塔尔公牛冷冻精液中精子的进步性、DNA片段化和顶体状态无显著差异(P < 0.05)。相比之下,巴厘公牛冷冻精液中精子全质膜含量显著高于西门塔尔公牛(P<0.05)。结果表明,从进步性、DNA断裂和精子顶体状态等指标来看,两种公牛品种的冷冻精液质量相同。同时,从精子完整的质膜来看,巴厘公牛的冷冻精液质量优于西门塔尔公牛。关键词:人工授精,冷冻精液质量,巴厘公牛和西门塔尔公牛
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引用次数: 0
Availability of Production Inputs in Broiler Partnership In Sleman Regency Yogyakarta 日惹Sleman Regency肉鸡伙伴关系生产投入的可用性
Pub Date : 2022-12-30 DOI: 10.20956/hajas.v4i2.20268
T. A. Kusumastuti, R. Widiati
Broiler has now developed a pattern of partnership. This study aims to determine the characteristics of farmers, businesses, and the availability of production input in broiler partnerships. The research was conducted in Sleman Regency. Location in 3 sub-districts i.e. Pakem, Cangkringan, and Ngemplak. The number of samples of 64 respondents and the core of 5 companies was performed by the purposive method. The analysis was carried out descriptively and the results of the analysis were written in tabular form. The results showed that farmers belonged to the productive age (45 years), education senior high school (43.42%), experience (11.15 years), and basic work as farmers. The business scale was 6014 heads/ period with a maintenance period of 35-36 days/periods/year. The maintenance system was an open house (81.58%). In the partnership agreement, the availability, amount, and price of input come from the core. A relationship between business characteristics and the provision of production inputs in the broiler partnership pattern and the results were still not optimal. The improvement and review of contents of the contract need to be reviewed to generate benefits and justice.Keywords: broiler, production input, partnership system
肉鸡现在已经发展出一种伙伴关系模式。本研究旨在确定肉鸡合作伙伴关系中农民、企业和生产投入的可用性的特征。这项研究是在Sleman Regency进行的。地点在3个街道,即巴基姆,仓克林安和根普乐。样本数量为64个,核心为5家公司,采用目的法进行。分析是描述性的,分析结果以表格形式写出来。结果表明:农户的生产年龄(45岁)、文化程度(43.42%)、经历(11.15年)、基本工作为农民;业务规模6014头/期,维护期35-36天/期/年。维修系统为开放参观(81.58%)。在合作协议中,投入的可用性、数量和价格来自核心。在肉鸡合作模式下,企业特征与生产投入提供之间的关系和结果仍然不是最优的。合同内容的改进和审查需要通过审查来产生利益和公正。关键词:肉鸡,生产投入,合作制
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引用次数: 0
Evaluation of Color, Hardness, and Tannin Content of Soft Cheese with Biduri Leaf Extract Level 用比杜丽叶提取物水平评价软奶酪的颜色、硬度和单宁含量
Pub Date : 2022-12-07 DOI: 10.20956/hajas.v4i2.21903
Sulmiyati Natsir, G. Malelak
Soft cheese Suspesi is a soft cheese to which biduri leaf extract is added as a coagulant. There is no research on the color, hardness, and content of tannins contained in soft cheese suspesi. The purpose of this research was to analyze and evaluate the hardness, color *L, *a, *b, and tannin content with the addition of biduri leaf extract at levels of 3% and 5% in soft cheese suspesi. This research used a completely randomized design with two treatments with six replications. A1= Biduri leaf extract level 3% (v/v), A2= Biduri leaf extract level 5% (v/v). Data on hardness, color, and tannin content were analyzed by Independent T-Test. The results showed a significant difference (P<0.05) in the hardness, color *L, and tannin content. Color *a and color *b did not show a significant difference (P>0.05). In conclusion, the levels of added biduri leaf extract gave different levels of hardness, color *L, *a, *b, and tannin content in the soft cheese suspesi. The characteristics of the soft cheese suspesi increased in terms of hardness, color *L, *a, *b, and tannin content in line with the level of added biduri leaf extract.
软奶酪是一种软奶酪,加入比杜丽叶提取物作为凝固剂。目前还没有关于软奶酪悬浮中的颜色、硬度和单宁含量的研究。本研究的目的是分析和评价在添加3%和5%的比杜丽叶提取物时软奶酪悬浮液的硬度、颜色*L、*a、*b和单宁含量。本研究采用完全随机设计,2个处理,6个重复。A1= Biduri叶提取物浓度3% (v/v), A2= Biduri叶提取物浓度5% (v/v)。硬度、颜色、单宁含量数据采用独立t检验分析。结果显示差异有统计学意义(P0.05)。综上所述,比杜丽叶提取物的添加量对软干酪悬浮液的硬度、颜色*L、*a、*b和单宁含量有不同程度的影响。软干酪悬浮液的硬度、颜色*L、*a、*b和单宁含量均随比杜丽叶提取物添加量的增加而增加。
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引用次数: 0
Reproductive Efficiency of Cows in Different Parity 不同胎次奶牛的繁殖效率
Pub Date : 2022-12-06 DOI: 10.20956/hajas.v4i2.20995
Mutmainna Mutmainna, S. Baco, H. Hasbi
Livestock is one of the agricultural sectors that plays an important role in providing animal products. Cows are one of the largest meat and dairy producers. The low reproductive efficiency of cattle is the biggest problem in terms of its development. Increasing reproductive efficiency through artificial insemination programs is one way to enhance livestock populations. Reproductive efficiency parameters can be measured through values of Service per Conception (S/C) and Conception Rate (CR). Service per conception and conception rate are related to cows parity. Parity is the number of calf that have been born to a cow. This study aimed to provide information on reproductive efficiency as seen from the value of S/C and CR of cows in different parieties and the factors that influence them. High rates of parity followed by high S/C score and low CR. Each animal has various values of S/C and CR. The normal range for S/C is 1.6-2.0 and 60% in CR. Several factors that affect reproductive efficiency are environment, nutrition, Body Condition Score (BCS), knowledge of farmers, inseminator skills and quality of semen used. The highest service per conception (S/C) value was at parity 5 with aged ± 7 years and the conception rate (CR) was at parity 3 aged ± 5 years.Keywords: should be written in no more than 5 (five) words or phrases.
畜牧业是农业部门之一,在提供动物产品方面发挥着重要作用。奶牛是最大的肉类和奶制品生产商之一。牛的繁殖效率低是影响其发展的最大问题。通过人工授精计划提高繁殖效率是增加牲畜数量的一种方法。生殖效率参数可通过每次受孕服务(S/C)和受孕率(CR)值来衡量。单胎服务次数和受胎率与奶牛胎次有关。胎次是指一头母牛所生的小牛的数量。本研究旨在从不同品种奶牛的S/C和CR值及其影响因素方面了解奶牛的繁殖效率。每种动物的S/C和CR值不同,S/C正常值为1.6 ~ 2.0,CR正常值为60%,影响繁殖效率的因素有环境、营养、体况评分(BCS)、农户知识、授精者技能和所用精液质量等。每次受胎服务(S/C)值在胎次5±7岁时最高,受胎率(CR)在胎次3±5岁时最高。关键词:不超过5个单词或短语。
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引用次数: 2
Aflatoxin M1 in Milk: Occurrence and Its Risk Association: A Review 牛奶中的黄曲霉毒素M1:发生及其风险关联:综述
Pub Date : 2022-11-15 DOI: 10.20956/hajas.v4i2.22097
Suliman Mohamed Yousof Sadam, R. Malaka, S. Baco, J. Mustabi
Aflatoxin M1 (AFM1) is the main secondary metabolites of aflatoxin B1 (AFB1) framed in the liver and discharged into milk when people and animals polish off AFB1-polluted food. The persist from feed to milk in dairy cows is affected by different dietary and physiological elements, including taking care of regimens, rate of ingestion, rate of absorption, animal health, hepatic biotransformation limit, and real milk production of the animals. AFM1 exposure might cause both intense and constant toxicity. In humans, AFM1 exposure is related with cancer-causing nature, genotoxicity, mutagenicity, and teratogenicity. In dairy animals, persistent openness to AFs can decrease execution, debilitate liver capability, compromise invulnerable capability, and increment sickness susceptibility. AFM1 openness represents a worry for the worldwide populace, especially for babies and youngsters who drink milk in bounteous amounts and thus, are more powerless to unfavorable impacts. The identification and quantification of AFs represents a critical test in food handling confirmation, since even a low AF concentration is hazardous for people and domesticated animals. Consistent human openness through dietary courses has prompted the burden of most extreme cut off points for AFM1 in milk and dairy products, taking into account that this is a gathering of products with exorbitant premium, particularly for babies and youngsters. Likewise, as per different examinations it is additionally very much felt that youthful animals are additionally observed to be more vulnerable to aflatoxin than grown-ups. Consequently; the defilement of cow milk and milk products by AFM1 ought to be perceived as unfortunate for youthful human and animals. Thus, the point of this paper is to review the occurrence of aflatoxin and its adverse results on animals and human health over the time.
黄曲霉毒素M1 (AFM1)是黄曲霉毒素B1 (AFB1)的主要次级代谢物,存在于肝脏中,并在人和动物食用受AFB1污染的食物时排入牛奶中。奶牛从饲料到牛奶的持续时间受不同的饮食和生理因素的影响,包括护理方案、摄食速度、吸收率、动物健康、肝脏生物转化极限和动物的实际产奶量。AFM1暴露可能引起强烈和持续的毒性。在人类中,AFM1暴露与致癌性、遗传毒性、诱变性和致畸性有关。在奶牛中,持续暴露于AFs会降低执行力,削弱肝脏功能,损害抗损伤能力,增加疾病易感性。AFM1的开放性代表了全世界民众的担忧,特别是对于大量饮用牛奶的婴儿和青少年来说,他们对不利的影响更无能为力。AF的鉴定和定量是食品处理确认中的一项关键测试,因为即使是低AF浓度也对人和家畜有害。考虑到这是一种溢价过高的产品,特别是对婴儿和青少年来说,人类在饮食过程中一贯的开放性,促使牛奶和乳制品中AFM1的最极端切断点的负担。同样,根据不同的研究,我们还非常清楚地看到,年轻的动物比成年动物更容易受到黄曲霉毒素的侵害。因此;对年轻的人类和动物来说,AFM1污染牛奶和奶制品应该被认为是不幸的。因此,本文的重点是回顾黄曲霉毒素的发生及其对动物和人类健康的不良影响。
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引用次数: 0
The Quality of Bali Bull Sexed Sperm Using Soybean Extract Extender 大豆萃取剂对巴厘公牛性精子质量的影响
Pub Date : 2022-07-25 DOI: 10.20956/hajas.v4i1.19439
Nur Eni Nur, A. Toleng, M. Yusuf
The process of sexing spermatozoa requires a medium that is able to protect and provide an optimal environment. One of them is extender media that is used to extend the volume of semen. The extender that is commonly used is commercially but some limitation such as price and availability. Therefore, it is necessary to have an alternative extender such as soybean. The aim of this study was to know the quality of sexed sperms diluted using soybean extender. Semen of Bali bull was collected for five times and then subjected to three different extender treatments after sexing; T1 (Soybean), T2 (Tris), T3 (Tris-soybean), and T0 (Fresh semen-tris) was used as control before sexing. The parameters observed were the quality of fresh semen and after sexing. The results of the study showed that the characteristic of Bali bull fresh semen macroscopically in this study was 4.75 mL in volume, cream color, distinctive odor, pH 6.6, moderate consistency, and microscopically motility, viability, abnormality, and concentration were 94.22%, 96.06% 4.89, and 1596×106, respectively. Motility and viability after sexing were significantly (P<0.05) decrease in comparison to the fresh semen (T0) at each treatment both top and bottom layers. In conclusion, the smallest decreased of the sperms motility and viability were still greater than 50%. Different extenders as one of the treatments in the present study did not showing different motility and viability of the sperms. This suggests that the use of tris and soybean or their combination can be used as semen extender for Bali bull sexed semen.Keywords: Soy extender, Bali bull, sexing, motility, viability.
精子的性别过程需要一种能够保护和提供最佳环境的培养基。其中之一是用于扩大精液体积的扩展介质。通常使用的扩展器是商业化的,但有一些限制,如价格和可用性。因此,有必要选择大豆等替代增稠剂。本研究的目的是了解用大豆扩展剂稀释的有性精子的质量。采集巴厘公牛5次精液,进行3次不同的延长处理;分别以T1(大豆)、T2 (Tris)、T3 (Tris-大豆)和T0(新鲜精液- Tris)为对照。所观察的参数为新鲜精液质量和性别化后精液质量。研究结果表明,本研究中巴厘公牛鲜精液的宏观特征为4.75 mL,其体积、乳白色、独特气味、pH值6.6、稠度适中,微观活力、活力、异常和浓度分别为94.22%、96.06%、4.89和1596×106。与新鲜精液(T0)相比,各处理(顶层和底层)的活力和活力均显著(P<0.05)降低。综上所述,精子活力和活力的最小降幅仍大于50%。作为本研究的一个处理,不同的扩展剂对精子的活力和活力没有表现出不同的影响。这表明大豆和大豆或两者组合可作为巴厘公牛有性精子的增精剂。关键词:大豆膨化剂,巴厘公牛,性别,活力,活力。
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引用次数: 0
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Hasanuddin Journal of Animal Science (HAJAS)
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