Sari Wulan Asih, T. Nurseta, K. Handono, H. Yudhanto
Women's issues are primarily related to infertility. This study aimed to demonstrate that ovarian healing in wistar rats exposed to cigarette smoke was impacted by the administration of extracted black garlic. This research uses True Experimental methods in vivo in the laboratory to identify the cause and effect of the variables being tested. This method involves replication, randomization, and control. The design chosen was a post-test-only control group design, where the experimental group received treatment and the control group did not, without random selection. The results of the study showed that there was no effect due to exposure to cigarette smoke with an increase in cortisol and a decrease in the number of secondary follicles in the ovaries of female wistar rats as well as the administration of Black Garlic (Allium sativum) extract. Black garlic extract unaffected cortisol levels and the number of secondary follicles or repair of the ovaries due to exposure to cigarette smoke. Further research is needed in dosing black garlic extract or combining it with other ingredients to provide effective results.
{"title":"Negative Effect of Cigarette Smoke: Black Garlic Opportunities for Prevention of Ovulation Disorders","authors":"Sari Wulan Asih, T. Nurseta, K. Handono, H. Yudhanto","doi":"10.31964/mltj.v10i1.585","DOIUrl":"https://doi.org/10.31964/mltj.v10i1.585","url":null,"abstract":"Women's issues are primarily related to infertility. This study aimed to demonstrate that ovarian healing in wistar rats exposed to cigarette smoke was impacted by the administration of extracted black garlic. This research uses True Experimental methods in vivo in the laboratory to identify the cause and effect of the variables being tested. This method involves replication, randomization, and control. The design chosen was a post-test-only control group design, where the experimental group received treatment and the control group did not, without random selection. The results of the study showed that there was no effect due to exposure to cigarette smoke with an increase in cortisol and a decrease in the number of secondary follicles in the ovaries of female wistar rats as well as the administration of Black Garlic (Allium sativum) extract. Black garlic extract unaffected cortisol levels and the number of secondary follicles or repair of the ovaries due to exposure to cigarette smoke. Further research is needed in dosing black garlic extract or combining it with other ingredients to provide effective results.","PeriodicalId":32131,"journal":{"name":"Medical Laboratory Technology Journal","volume":" 104","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141367923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to determine how the effect of flavonoid phaleria macrocarpa fruit extract on the endometrial thickness of Mus musculus which is used as an experimental animal with a menopause model. This study employed a randomized post-test-only control group design on 32 female mice. Ovariectomy simulated menopause, followed by treatment with flavonoid from Phaleria macrocarpa fruit extract. Post-observariectomized, follicle-stimulating hormone levels indicated menopausal status. Hematoxylin-Eosin methods assessed endometrial thickness. The result indicated reduced endometrial thinning in the positive control group (K+). Conversely, the treatment group (P1-P4) exhibited increased endometrial thickness post-flavonoid extract administration. Ovariectomized-induced estrogen decline resulted in reduced endometrial thinning, mimicking menopausal conditions. Statistically, flavonoid extract administration increased endometrial thickness in ovariectomized-induced menopausal mice, with optimal effect observed at 7.5 mg/mice/day for 14 days. The preferred intervention dose for altering endometrial thickness in menopausal conditions was identified as P1 (3.75 mg/mice/day). In conclusion, a flavonoid from Phaleria macrocarpa fruit extract mitigates endometrial thinning in the menopausal mice model. For implication in menopausal women, clinical trials need to be conducted to evaluate the dosage of these flavonoids for their potential as a natural SERM (Selective Estrogen Receptor Modulator) before clinical application in managing endometrial health. Additionally, research on molecular effects particularly regarding ER (Estrogen Receptors) in the endometrial organ, is needed to confirm their phytoestrogenic effect.
{"title":"The Impact of Phaleria macrocarpa Fruit Flavonoid Extract on Endometrial Thickness in Mice Menopausal Model","authors":"Dyah Ayu Septika Wijaya, Recavery Wulandari, Sutrisno Sutrisno, Yahya Irwanto, Kenty W. Anita","doi":"10.31964/mltj.v10i1.584","DOIUrl":"https://doi.org/10.31964/mltj.v10i1.584","url":null,"abstract":"This study aims to determine how the effect of flavonoid phaleria macrocarpa fruit extract on the endometrial thickness of Mus musculus which is used as an experimental animal with a menopause model. This study employed a randomized post-test-only control group design on 32 female mice. Ovariectomy simulated menopause, followed by treatment with flavonoid from Phaleria macrocarpa fruit extract. Post-observariectomized, follicle-stimulating hormone levels indicated menopausal status. Hematoxylin-Eosin methods assessed endometrial thickness. The result indicated reduced endometrial thinning in the positive control group (K+). Conversely, the treatment group (P1-P4) exhibited increased endometrial thickness post-flavonoid extract administration. Ovariectomized-induced estrogen decline resulted in reduced endometrial thinning, mimicking menopausal conditions. Statistically, flavonoid extract administration increased endometrial thickness in ovariectomized-induced menopausal mice, with optimal effect observed at 7.5 mg/mice/day for 14 days. The preferred intervention dose for altering endometrial thickness in menopausal conditions was identified as P1 (3.75 mg/mice/day). In conclusion, a flavonoid from Phaleria macrocarpa fruit extract mitigates endometrial thinning in the menopausal mice model. For implication in menopausal women, clinical trials need to be conducted to evaluate the dosage of these flavonoids for their potential as a natural SERM (Selective Estrogen Receptor Modulator) before clinical application in managing endometrial health. Additionally, research on molecular effects particularly regarding ER (Estrogen Receptors) in the endometrial organ, is needed to confirm their phytoestrogenic effect.","PeriodicalId":32131,"journal":{"name":"Medical Laboratory Technology Journal","volume":" 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141372693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infertility cases in the world are increasing in 2020, with 186 million individuals affected worldwide. The most common cause of male infertility is due to decreased sperm quality. Sperm membrane integrity is known to play an essential role in the fertilization ability of spermatozoa. The methanol fraction of Luvunga sarmentosa root extract has the highest antioxidant activity compared to other fractions and can improve sperm motility in vitro. This study aims to determine the effect of the methanol fraction of Luvunga sarmentosa root extract on human sperm membrane integrity and the effective concentration of Luvunga sarmentosa methanol extract fraction to improve sperm membrane integrity in vitro. The samples used were washed human spermatozoa in vitro, then added BWW medium, and incubated at 37℃ for 1 hour with methanol fraction of Luvunga sarmentosa root extract at concentrations of 100, 500, 1000, and 5000ng/ml as well as the control group (BWW only). Furthermore, 100uL of each sample was added to 1mL of Hypo-osmotic swelling (HOS) solution, then incubated at 37oC for 30 minutes to see the integrity of the spermatozoa membrane from the tail swelling. The results showed that sperm membrane integrity significantly increased at 100, 500, 1000, and 5000ng/mL concentrations compared to the control group. The methanol fraction of Luvunga sarmentosa root extract was most effective at a concentration of 100ng/mL. Methanol fraction of Luvunga sarmentosa root extract can improve human sperm membrane integrity.
{"title":"The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty","authors":"Alya Firyal Nuha, Silvani Permatasari, Arini Ratnasari, Ratna Widayati, Rian Ka Praja","doi":"10.31964/mltj.v10i1.571","DOIUrl":"https://doi.org/10.31964/mltj.v10i1.571","url":null,"abstract":"Infertility cases in the world are increasing in 2020, with 186 million individuals affected worldwide. The most common cause of male infertility is due to decreased sperm quality. Sperm membrane integrity is known to play an essential role in the fertilization ability of spermatozoa. The methanol fraction of Luvunga sarmentosa root extract has the highest antioxidant activity compared to other fractions and can improve sperm motility in vitro. This study aims to determine the effect of the methanol fraction of Luvunga sarmentosa root extract on human sperm membrane integrity and the effective concentration of Luvunga sarmentosa methanol extract fraction to improve sperm membrane integrity in vitro. The samples used were washed human spermatozoa in vitro, then added BWW medium, and incubated at 37℃ for 1 hour with methanol fraction of Luvunga sarmentosa root extract at concentrations of 100, 500, 1000, and 5000ng/ml as well as the control group (BWW only). Furthermore, 100uL of each sample was added to 1mL of Hypo-osmotic swelling (HOS) solution, then incubated at 37oC for 30 minutes to see the integrity of the spermatozoa membrane from the tail swelling. The results showed that sperm membrane integrity significantly increased at 100, 500, 1000, and 5000ng/mL concentrations compared to the control group. The methanol fraction of Luvunga sarmentosa root extract was most effective at a concentration of 100ng/mL. Methanol fraction of Luvunga sarmentosa root extract can improve human sperm membrane integrity.","PeriodicalId":32131,"journal":{"name":"Medical Laboratory Technology Journal","volume":" July","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141127752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fajar Dwi Prastiwi, Sutini Lamadi, Husnul Khotimah, I. Wiyasa, Tri Yudani M. Raras
Gestational Diabetes Mellitus (GDM) is diabetes that occurs during pregnancy. GDM is characterized by hyperglycemia. Hyperglycemia causes an inflammatory response characterized by the excretion of pro-inflammatory cytokines, one of which is IL-6. Centella asiatica and Rosmarinus officinalis are known as anti-diabetic and anti-inflammatory. This study aims to study the effect of the combination of nanoemulsion Centella asiatica and Rosmarinus officinalis on the expression of Interleukin-6 in Zebrafish larvae induced by 3% glucose. Hyperglycemia is known from increased levels of Phosphoenolpyruvate carboxykinase in Zebrafish larvae. Phosphoenolpyruvate carboxykinase and Interleukin-6 expression were measured by conventional PCR method. The combination of Rosmarinus officinalis nanoemulsion and Centella asiatica was given with three different doses, namely at a concentration of 2.5, 5 and 10 µg/mL. The statistical analysis used was One Way ANOVA to measure Interleukin-6 expression levels. The results of this study prove that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions reduces the expression of Interleukin-6. The dose closest to the negative control was at a concentration of 5 μg/mL, although the final result was statistically significant (p<0.05). In conclusion, combining nanoemulsion Centella asiatica and Rosmarinus officinalis with the right dose reduced levels of Interleukin 6 in zebrafish larvae induced by high glucose.
{"title":"Rosmarinus officinalis and Centella asiatica Affect Interleukin-6 Zebrafish Larvae Induced High Glucose","authors":"Fajar Dwi Prastiwi, Sutini Lamadi, Husnul Khotimah, I. Wiyasa, Tri Yudani M. Raras","doi":"10.31964/mltj.v10i1.538","DOIUrl":"https://doi.org/10.31964/mltj.v10i1.538","url":null,"abstract":"Gestational Diabetes Mellitus (GDM) is diabetes that occurs during pregnancy. GDM is characterized by hyperglycemia. Hyperglycemia causes an inflammatory response characterized by the excretion of pro-inflammatory cytokines, one of which is IL-6. Centella asiatica and Rosmarinus officinalis are known as anti-diabetic and anti-inflammatory. This study aims to study the effect of the combination of nanoemulsion Centella asiatica and Rosmarinus officinalis on the expression of Interleukin-6 in Zebrafish larvae induced by 3% glucose. Hyperglycemia is known from increased levels of Phosphoenolpyruvate carboxykinase in Zebrafish larvae. Phosphoenolpyruvate carboxykinase and Interleukin-6 expression were measured by conventional PCR method. The combination of Rosmarinus officinalis nanoemulsion and Centella asiatica was given with three different doses, namely at a concentration of 2.5, 5 and 10 µg/mL. The statistical analysis used was One Way ANOVA to measure Interleukin-6 expression levels. The results of this study prove that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions reduces the expression of Interleukin-6. The dose closest to the negative control was at a concentration of 5 μg/mL, although the final result was statistically significant (p<0.05). In conclusion, combining nanoemulsion Centella asiatica and Rosmarinus officinalis with the right dose reduced levels of Interleukin 6 in zebrafish larvae induced by high glucose.","PeriodicalId":32131,"journal":{"name":"Medical Laboratory Technology Journal","volume":" 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141127968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Paramita Prastiwi, Leni Ria Ariana, Gema Alya Salsabila, Sutrisno Sutrisno, N. Nurdiana, N. Setijowati, I. W. A. Indrawan
Endometriosis is an inflammatory disease that occurs in glands outside the endometrium. Interleukin 17 (IL-17) is a proinflammatory cytokine degrading Th17 cells, which play an essential role in several inflammatory diseases. IL-17 expression is upregulated in serum, peritoneal fluid (PF), and endometriotic lesions from patients with endometriosis. Usually, treatment for endometriosis is dienogest. However, it has many side effects if used long-term. Fruit and seeds of Phaleria macrocarpa have various critical biological activities in the form of extracts such as antimicrobial, anti-inflammatory, and antioxidant activities. This study aims to analyze the effect of comparing flavonoid fruit extract of Phaleria macrocarpa and dienogest on IL-17A levels in endometriosis mice. A total of 35 endometriosis model mice were divided into seven groups, namely the group of healthy mice (without any treatment), the endometriosis group, and the endometriosis group, which were given the treatment of flavonoid fruit extracts of Phaleria macrocarpa and dienogest. The sample used in this study was peritoneal fluid. The enzyme-linked immunosorbent assay (ELISA) measured IL-17A levels in each observation group. The hypothesis was tested using One-Way ANOVA analysis with SPSS software version 26. This study found a significant difference (p<0.05) between the mean values of the positive control group and the negative control group. There was also a significant difference (p<0.05) in the mean value IL-17 between the treatment groups that were given sphalerite macrocarpa and dienogest extracts. Flavonoid fruit extract of Phaleria macrocarpa had a significant effect on reducing IL-17A levels in endometriosis model mice, and there is a significant difference with dienogest. Thus, the flavonoid fruit extract of Phaleria macrocarpa can potentially treat endometriosis by decreasing inflammatory factors.
{"title":"Potential Of Mahkota Dewa Fruit (Phaleria macrocarpa) Flavonoids Extract and Dienogest to Reduce IL-17A Levels in Mice Endometriosis Model","authors":"Ana Paramita Prastiwi, Leni Ria Ariana, Gema Alya Salsabila, Sutrisno Sutrisno, N. Nurdiana, N. Setijowati, I. W. A. Indrawan","doi":"10.31964/mltj.v9i2.536","DOIUrl":"https://doi.org/10.31964/mltj.v9i2.536","url":null,"abstract":"Endometriosis is an inflammatory disease that occurs in glands outside the endometrium. Interleukin 17 (IL-17) is a proinflammatory cytokine degrading Th17 cells, which play an essential role in several inflammatory diseases. IL-17 expression is upregulated in serum, peritoneal fluid (PF), and endometriotic lesions from patients with endometriosis. Usually, treatment for endometriosis is dienogest. However, it has many side effects if used long-term. Fruit and seeds of Phaleria macrocarpa have various critical biological activities in the form of extracts such as antimicrobial, anti-inflammatory, and antioxidant activities. This study aims to analyze the effect of comparing flavonoid fruit extract of Phaleria macrocarpa and dienogest on IL-17A levels in endometriosis mice. A total of 35 endometriosis model mice were divided into seven groups, namely the group of healthy mice (without any treatment), the endometriosis group, and the endometriosis group, which were given the treatment of flavonoid fruit extracts of Phaleria macrocarpa and dienogest. The sample used in this study was peritoneal fluid. The enzyme-linked immunosorbent assay (ELISA) measured IL-17A levels in each observation group. The hypothesis was tested using One-Way ANOVA analysis with SPSS software version 26. This study found a significant difference (p<0.05) between the mean values of the positive control group and the negative control group. There was also a significant difference (p<0.05) in the mean value IL-17 between the treatment groups that were given sphalerite macrocarpa and dienogest extracts. Flavonoid fruit extract of Phaleria macrocarpa had a significant effect on reducing IL-17A levels in endometriosis model mice, and there is a significant difference with dienogest. Thus, the flavonoid fruit extract of Phaleria macrocarpa can potentially treat endometriosis by decreasing inflammatory factors.","PeriodicalId":32131,"journal":{"name":"Medical Laboratory Technology Journal","volume":"13 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139161350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Globally, 48 million couples experience infertility, where male infertility factors contribute to 50% of cases. Spermatozoa motility is a crucial parameter in assessing male fertility. Antioxidants act as the body's defence against excessive ROS and can be used as a treatment for male infertility. One of the local plants in Central Kalimantan that is potentially rich in antioxidants is Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb. However, there is limited research on the bioactivity of Bajakah Kalalawit on spermatozoa in vitro. This study aims to determine the effect of ethanol extract of Uncaria gambir (W. Hunter) Roxb on spermatozoa motility in vitro. Bajakah Kalalawit stems were extracted using a 3x24-hour maceration technique with 96% ethanol. After obtaining the concentrated extract, the secondary metabolite compound content was analyzed through a phytochemical screening. The sample used was in vitro human spermatozoa that were washed and added to Bigger Whitten Whittingham medium, then incubated at 37°C for 1 hour with a 96% ethanol extract of Bajakah Kalalawit at doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml, as well as a control group (Bigger, Whitten & Whittingham medium only). The results showed that the compounds contained in the ethanol extract of Uncaria gambir (W.Hunter) Roxb were terpenoids, flavonoids, phenolics, steroids, saponins, alkaloids, and tannins. Spermatozoa motility significantly increased in the treatment groups starting from doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml compared to the control group. Ethanol extract of Uncaria gambir (W.Hunter) Roxb could increase spermatozoa motility in vitro and succeeded in improving reproductive technology.
{"title":"Bioactivity Examination of Uncaria gambir (W.Hunter) Roxb on In Vitro Human Sperm Motility","authors":"Silvani Permatasari, Nida Halisa, Agnes Frethernety","doi":"10.31964/mltj.v9i2.563","DOIUrl":"https://doi.org/10.31964/mltj.v9i2.563","url":null,"abstract":"Globally, 48 million couples experience infertility, where male infertility factors contribute to 50% of cases. Spermatozoa motility is a crucial parameter in assessing male fertility. Antioxidants act as the body's defence against excessive ROS and can be used as a treatment for male infertility. One of the local plants in Central Kalimantan that is potentially rich in antioxidants is Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb. However, there is limited research on the bioactivity of Bajakah Kalalawit on spermatozoa in vitro. This study aims to determine the effect of ethanol extract of Uncaria gambir (W. Hunter) Roxb on spermatozoa motility in vitro. Bajakah Kalalawit stems were extracted using a 3x24-hour maceration technique with 96% ethanol. After obtaining the concentrated extract, the secondary metabolite compound content was analyzed through a phytochemical screening. The sample used was in vitro human spermatozoa that were washed and added to Bigger Whitten Whittingham medium, then incubated at 37°C for 1 hour with a 96% ethanol extract of Bajakah Kalalawit at doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml, as well as a control group (Bigger, Whitten & Whittingham medium only). The results showed that the compounds contained in the ethanol extract of Uncaria gambir (W.Hunter) Roxb were terpenoids, flavonoids, phenolics, steroids, saponins, alkaloids, and tannins. Spermatozoa motility significantly increased in the treatment groups starting from doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml compared to the control group. Ethanol extract of Uncaria gambir (W.Hunter) Roxb could increase spermatozoa motility in vitro and succeeded in improving reproductive technology.","PeriodicalId":32131,"journal":{"name":"Medical Laboratory Technology Journal","volume":"197 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139161070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Edusola Juliana Olaniyan, Mathias Abiodun Emokpae, Fidelis Ohiremen Oyakhire, Ahmed Liasu Adeagbo, Iria Kelly Esezobor, Stephen Olawale Olaniyan
The vasomotor symptoms of menopause, including hot flashes, sweating, physical and psychological discomfort, and emotional changes, are accurate and experienced by many of the menopausal and postmenopausal female population. In addition, it causes osteoporosis and slowed metabolism, raising the chance of developing many different ailments. Given that hormone replacement therapy (HRT) has been linked to an increased cancer risk, this investigation was undertaken to identify viable alternatives. The study aimed to investigate the impact of Soybean phytoestrogen-rich extract on some markers of inflammation of 4-vinyl cyclohexene diepoxide-induced menopause in albino rats. Sixty-five (65) female albino Wistar rats were employed in the investigation. Each one was induced with 80mg/kg of 4-vinyl cyclohexene diepoxide before being treated with either normal estradiol therapy (14ug/kg) or varying concentrations of the soybean phytoestrogen-rich extract (200 mg/kg, 400 mg/kg, and 600 mg/kg). Inflammatory markers (C-reactive protein (CRP), interleukin-6 (IL-6), and tumour necrosis factor-alpha (TNF-α)) were measured by ELISA methods. Statistical software SPSS (IBM) version 23.0 was used to analyze the data. Compared to the positive control group, the soybean phytoestrogen-rich extract therapy group saw a dose-dependent reduction in CRP and IL-6 levels (p<0.05) but not in TNF-α (P>0.05). Data from this research demonstrate the anti-inflammatory effects of soybean phytoestrogen-rich extract therapy in menopause-induced female Wistar rats. Soybean phytoestrogen-rich extract therapy in a high dose appears to show no significant difference from hormone replacement therapy as an alternate estrogen source in managing inflammation as a chronic disease.
{"title":"Impact of Soybean Phytoestrogen-Rich Extract on Markers of Inflammation Markers in 4-Vinyl Cyclohexane Diepoxide-Induced Menopause in Albino Rats","authors":"Edusola Juliana Olaniyan, Mathias Abiodun Emokpae, Fidelis Ohiremen Oyakhire, Ahmed Liasu Adeagbo, Iria Kelly Esezobor, Stephen Olawale Olaniyan","doi":"10.31964/mltj.v9i2.549","DOIUrl":"https://doi.org/10.31964/mltj.v9i2.549","url":null,"abstract":"The vasomotor symptoms of menopause, including hot flashes, sweating, physical and psychological discomfort, and emotional changes, are accurate and experienced by many of the menopausal and postmenopausal female population. In addition, it causes osteoporosis and slowed metabolism, raising the chance of developing many different ailments. Given that hormone replacement therapy (HRT) has been linked to an increased cancer risk, this investigation was undertaken to identify viable alternatives. The study aimed to investigate the impact of Soybean phytoestrogen-rich extract on some markers of inflammation of 4-vinyl cyclohexene diepoxide-induced menopause in albino rats. Sixty-five (65) female albino Wistar rats were employed in the investigation. Each one was induced with 80mg/kg of 4-vinyl cyclohexene diepoxide before being treated with either normal estradiol therapy (14ug/kg) or varying concentrations of the soybean phytoestrogen-rich extract (200 mg/kg, 400 mg/kg, and 600 mg/kg). Inflammatory markers (C-reactive protein (CRP), interleukin-6 (IL-6), and tumour necrosis factor-alpha (TNF-α)) were measured by ELISA methods. Statistical software SPSS (IBM) version 23.0 was used to analyze the data. Compared to the positive control group, the soybean phytoestrogen-rich extract therapy group saw a dose-dependent reduction in CRP and IL-6 levels (p<0.05) but not in TNF-α (P>0.05). Data from this research demonstrate the anti-inflammatory effects of soybean phytoestrogen-rich extract therapy in menopause-induced female Wistar rats. Soybean phytoestrogen-rich extract therapy in a high dose appears to show no significant difference from hormone replacement therapy as an alternate estrogen source in managing inflammation as a chronic disease.","PeriodicalId":32131,"journal":{"name":"Medical Laboratory Technology Journal","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135968050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Past studies have delved into the genetic factors underlying anti-tuberculosis drug-induced liver injury (AT-DILI), primarily concentrating on polymorphisms in genes responsible for drug-metabolizing enzymes. However, the immune system's potential impact on drug adverse effects, specifically through genes such as HLA, has received limited attention. Previous research has notably revealed an association between HLA-DQB1*05 and AT-DILI, specifically the prevalence of the HLA-DQB*05:02 allele in AT-DILI patients. In light of this, our study aimed to investigate a potential link between HLA-DQB1*05:02 alleles and AT-DILI. In this study, we included 51 AT-DILI cases and 59 controls belonging to the Javanese ethnic group. The HLA-DQB1*05:02 genotypes were determined using a customized PCR-based typing method, and the results were further confirmed by analyzing five samples via the Luminex assay. Our findings revealed a significant association between HLA-DQA1*05: 02 and the risk of AT-DILI (P = 0.022; OR (95% CI) = 6.11 (1.25-29.74)). Moreover, the consistent results obtained from the Luminex assay validated the reliability of the custom PCR-based genotyping approach. This preliminary study sheds light on the relationship between the HLA-DQB1*05:02 allele and AT-DILI within the Indonesian population. Furthermore, our study demonstrates the dependability of custom PCR-based genotyping in detecting HLA-DQB1*05:02 alleles. Nevertheless, further research is imperative to corroborate and expand upon our findings.
{"title":"HLA-DQB1*05:02 Allele Association with Anti-Tuberculosis Drug Induced Liver Injury: A Single-Hospital Based Study in Jakarta, Indonesian Population","authors":"Kinasih Prayuni, Hilyatuz Zahroh, Syafrizal Syafrizal, Rika Yuliwulandari","doi":"10.31964/mltj.v9i2.547","DOIUrl":"https://doi.org/10.31964/mltj.v9i2.547","url":null,"abstract":"Past studies have delved into the genetic factors underlying anti-tuberculosis drug-induced liver injury (AT-DILI), primarily concentrating on polymorphisms in genes responsible for drug-metabolizing enzymes. However, the immune system's potential impact on drug adverse effects, specifically through genes such as HLA, has received limited attention. Previous research has notably revealed an association between HLA-DQB1*05 and AT-DILI, specifically the prevalence of the HLA-DQB*05:02 allele in AT-DILI patients. In light of this, our study aimed to investigate a potential link between HLA-DQB1*05:02 alleles and AT-DILI. In this study, we included 51 AT-DILI cases and 59 controls belonging to the Javanese ethnic group. The HLA-DQB1*05:02 genotypes were determined using a customized PCR-based typing method, and the results were further confirmed by analyzing five samples via the Luminex assay. Our findings revealed a significant association between HLA-DQA1*05: 02 and the risk of AT-DILI (P = 0.022; OR (95% CI) = 6.11 (1.25-29.74)). Moreover, the consistent results obtained from the Luminex assay validated the reliability of the custom PCR-based genotyping approach. This preliminary study sheds light on the relationship between the HLA-DQB1*05:02 allele and AT-DILI within the Indonesian population. Furthermore, our study demonstrates the dependability of custom PCR-based genotyping in detecting HLA-DQB1*05:02 alleles. Nevertheless, further research is imperative to corroborate and expand upon our findings.","PeriodicalId":32131,"journal":{"name":"Medical Laboratory Technology Journal","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134947945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Maliza, Hevi Horiza, S. Syukur, Allimuddin Tofrizal, Bramadi Arya
Kirsten rat sarcoma viral oncogene (KRAS) gene mutations lead to abnormal activation of the RAS signalling pathway and have been associated with poor prognosis and resistance to some therapeutics. This study aimed to identify mutation characteristics of the KRAS genes codon 12 and 13 in colorectal cancer patients in West Sumatra. KRAS mutations were analyzed in 20 DNA of colorectal cancer patients' tissue samples by using polymerase chain reaction (PCR) with specific primer and direct sequencing analysis. Our findings showed five samples (25%) with mutated KRAS at codons 12 and 13 (including three samples with GGT→GAT, one sample with GGT→GTT in codon 12, and one sample with GGC→GAC in codon 13). In conclusion, we found two variations of amino acid changes at codon 12 (G12D and G12V) and one at codon 13 (G13D). More research with many samples is required to obtain conclusive data on the relationship between these gene mutations and colorectal cancer response to therapy and prognosis.
{"title":"Identıfıcatıon of KRAS Mutatıons in Colorectal Carcınoma Patıents at Dr. M. Djamıl Hospıtal, West Sumatra-Indonesıa","authors":"R. Maliza, Hevi Horiza, S. Syukur, Allimuddin Tofrizal, Bramadi Arya","doi":"10.31964/mltj.v9i2.535","DOIUrl":"https://doi.org/10.31964/mltj.v9i2.535","url":null,"abstract":"Kirsten rat sarcoma viral oncogene (KRAS) gene mutations lead to abnormal activation of the RAS signalling pathway and have been associated with poor prognosis and resistance to some therapeutics. This study aimed to identify mutation characteristics of the KRAS genes codon 12 and 13 in colorectal cancer patients in West Sumatra. KRAS mutations were analyzed in 20 DNA of colorectal cancer patients' tissue samples by using polymerase chain reaction (PCR) with specific primer and direct sequencing analysis. Our findings showed five samples (25%) with mutated KRAS at codons 12 and 13 (including three samples with GGT→GAT, one sample with GGT→GTT in codon 12, and one sample with GGC→GAC in codon 13). In conclusion, we found two variations of amino acid changes at codon 12 (G12D and G12V) and one at codon 13 (G13D). More research with many samples is required to obtain conclusive data on the relationship between these gene mutations and colorectal cancer response to therapy and prognosis.","PeriodicalId":32131,"journal":{"name":"Medical Laboratory Technology Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44268028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sutini Lamadi, F.D. Prastiwi, Tri Yudani, Mardining Raras, H. Khotimah, Wayan Arsana Wiyasa
Gestational diabetes mellitus (GDM) is closely related to oxidative stress conditions in insulin resistance conditions that increase Reactive Oxygen Species (ROS) against the body's defense antioxidant mechanism. Chronic complications due to hyperglycemia in patients with GDM increase BAX / BCL2 levels which then activates the change of procaspase 3 to caspase 3, an activator of apoptosis. This study aims to determine the effect of the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions on caspase 3 expressions in zebrafish models of gestational diabetes mellitus, with a posttest-only controlled group design. The samples were divided into five groups, namely K- (EM), K+(EM+3% Glucose), P1, P2 and P3 (3% Glucose + Combination of Rosmarinus officinalis and Centella asiatica 2.5μg/ml, 5μg/ml and 10μg/ml), which will be tested for PEPCK and Caspase expression at the age of 3dpf using Real Time-PCR. The results of the correlation test of the group with caspase 3 obtained (p = 0.045) negative direction. These results show that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsion decreased Caspase 3 expression in zebrafish models of gestational diabetes mellitus. The combination of Rosmarinus officinalis and Centella asiatica nanoemulsion has the potential to reduce blood glucose levels and reduce the risk of apoptosis in gestational diabetes mellitus patients.
{"title":"Effectiveness of Rosmarinus officinalis and Centella asiatica Nanoemulsions Against Caspase 3 Gestational Diabetes Mellitus Expression","authors":"Sutini Lamadi, F.D. Prastiwi, Tri Yudani, Mardining Raras, H. Khotimah, Wayan Arsana Wiyasa","doi":"10.31964/mltj.v9i2.537","DOIUrl":"https://doi.org/10.31964/mltj.v9i2.537","url":null,"abstract":"Gestational diabetes mellitus (GDM) is closely related to oxidative stress conditions in insulin resistance conditions that increase Reactive Oxygen Species (ROS) against the body's defense antioxidant mechanism. Chronic complications due to hyperglycemia in patients with GDM increase BAX / BCL2 levels which then activates the change of procaspase 3 to caspase 3, an activator of apoptosis. This study aims to determine the effect of the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions on caspase 3 expressions in zebrafish models of gestational diabetes mellitus, with a posttest-only controlled group design. The samples were divided into five groups, namely K- (EM), K+(EM+3% Glucose), P1, P2 and P3 (3% Glucose + Combination of Rosmarinus officinalis and Centella asiatica 2.5μg/ml, 5μg/ml and 10μg/ml), which will be tested for PEPCK and Caspase expression at the age of 3dpf using Real Time-PCR. The results of the correlation test of the group with caspase 3 obtained (p = 0.045) negative direction. These results show that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsion decreased Caspase 3 expression in zebrafish models of gestational diabetes mellitus. The combination of Rosmarinus officinalis and Centella asiatica nanoemulsion has the potential to reduce blood glucose levels and reduce the risk of apoptosis in gestational diabetes mellitus patients.","PeriodicalId":32131,"journal":{"name":"Medical Laboratory Technology Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46400694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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