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Potential of frangipani flower (Plumeria sp.) as a source of antibacterial and antioxidants and its application in the pharmacological activities 佛甲草花(Plumeria sp.)作为抗菌剂和抗氧化剂来源的潜力及其在药理学活动中的应用
Pub Date : 2024-07-14 DOI: 10.24815/jn.v24i2.29356
K. P. Candra, Gisti Malinda Lestari, Sulistyo Prabowo, Yuliani Yuliani, M. Marwati, Maulida Rachmawati
Frangipani (Plumeria spp.) was a flowering ornamental plant with several metabolites that can be used as traditional medicine for various diseases such as laxative, anti-itch, anti-inflammatory, diabetic, and malaria. The Frangipani flower showed antibacterial activity against E. coli, P. aeruginosa, S. aureus, C. albicans, S. pyogenes, S. typhi, B. pumilus, B. subtilis, S. dysenteriae, P. mirabilis, P. fluorescens, B. cereus, S. flexneri, and M. furfur. Frangipani flowers also have high antioxidant activity due to their significant phenol and flavonoid content. This study provided an overview of the application of Frangipani flowers for pharmaceutical purposes.
佛手花(Plumeria spp.)是一种开花的观赏植物,具有多种代谢产物,可用作治疗各种疾病的传统药物,如泻药、止痒、消炎、糖尿病和疟疾。佛甲草花对大肠杆菌、绿脓杆菌、金黄色葡萄球菌、白喉杆菌、化脓性葡萄球菌、伤寒杆菌、普米氏杆菌、枯草杆菌、痢疾杆菌、奇异变形杆菌、荧光酸杆菌、蜡样芽孢杆菌、柔毛杆菌和糠秕孢子菌具有抗菌活性。由于其含有大量的酚类和类黄酮,佛甲草花还具有很高的抗氧化活性。这项研究概述了佛手花在制药方面的应用。
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引用次数: 0
Preparation and characterization of physical properties of molded pulp from empty fruit bunches of oil palm 油棕空果串模塑浆的制备和物理性质表征
Pub Date : 2024-07-14 DOI: 10.24815/jn.v24i2.37444
Rosliana Lubis, Riyanto Riyanto, Saisa Saisa
This research aims to determine the impact of pretreatment on empty fruit bunches (EFB) of palm oil fibers before the pulping process on the quality of the molded pulp produced. The study includes EFB fiber pretreatment processes, pulp production, pulp molding, physical characteristic analysis, and molded pulp performance evaluation. The pulp production process involves acid-base hydrolysis reactions. The produced molded pulp is done manually. Characterization of the molded pulp includes measurements of paper grammage, thickness, density, and moisture content. The characteristics of pulp produced from pretreated EFB fibers are as follows: an average grammage value of 415.48 g/m², paper thickness of 1.53 mm, water absorption capacity of 13.33-33.33%, density of paper of 0,026 x 106 - 0,027 x 106 g/m3, and a smooth surface morphology. Meanwhile, the characteristics of pulp produced from without pretreatment EFB fibers include an average grammage value of 372.18 g/m², paper thickness of 0.77 mm, water absorption capacity of 20.34-43.21%, density of paper of 0,004 x 106 - 0,008 x 106 g/m3, and a rough surface morphology due to pores on the fiber surface.
本研究旨在确定制浆工艺前对棕榈油纤维空果串(EFB)的预处理对所生产的模塑纸浆质量的影响。研究内容包括空果穗纤维预处理工艺、纸浆生产、纸浆模塑、物理特性分析和模塑纸浆性能评估。纸浆生产过程涉及酸碱水解反应。生产的模塑纸浆由人工完成。模塑纸浆的特性分析包括纸张克重、厚度、密度和含水量的测量。经预处理的 EFB 纤维制成的纸浆具有以下特征:平均克重为 415.48 克/平方米,纸张厚度为 1.53 毫米,吸水率为 13.33%-33.33%,纸张密度为 0,026 x 106 - 0,027 x 106 克/立方米,表面形态光滑。而未经预处理的 EFB 纤维制成的纸浆的特征包括平均克重为 372.18 g/m²,纸张厚度为 0.77 mm,吸水率为 20.34-43.21%,纸张密度为 0,004 x 106 - 0,008 x 106 g/m3,以及由于纤维表面的孔隙造成的粗糙表面形态。
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引用次数: 0
Oral glucose tolerance assay of extract from Mangifera foetida l. and Pandanus amaryllifolius roxb. leaves Mangifera foetida l.和 Pandanus amaryllifolius roxb.叶提取物的口服葡萄糖耐量试验
Pub Date : 2024-02-23 DOI: 10.24815/jn.v24i1.30982
Y. Retnaningtyas, Fransiska Maria Cristianty, Nia Kristiningrum, Pramudia Wardani
This study examines the potential antidiabetic activity of a combination of Mangifera foetida L. and Pandanus amaryllifolius Roxb. leaves. Mangifera leaves contain a compound called mangiferin, which acts as an antidiabetic and antioxidant. On the other hand, Pandanus leaves, are rich in terpenoids and steroids that also possess anti-diabetic effects. An Oral Glucose Tolerance Test (OGTT) was conducted on male mice of the Babl/c strain to assess the antidiabetic effects. The test involved administering a combination of ethanolic leaf extracts from Mangifera foetida L. and Pandanus amaryllifolius Roxb. in a 1:1 ratio. The study followed a pre-test design with a control group, comprising six treatment groups, each consisting of four male mice. The groups were described as follows: Group 1 was served as the normal group without any treatment. Groups II-IV received single extracts or combinations of extracts at doses of 62.5 mg/200g BW, 125 mg/200g BW, and 250 mg/200g BW. Group V acted as the positive control and was given oral glucose and glibenclamide at a dose of 0.09 mg/200g BW. Group VI was served as the negative control, receiving oral glucose and distilled water. The group that exhibited the highest percentage of decreased blood sugar levels was the one given oral glucose at a dose of 0.078g/20g BW of mice, along with the combination of Mangifera foetida L. and Pandanus amaryllifolius Roxb. leaves extract (1:1) at a dose of 35 mg/20g BW. This group demonstrated a decrease of 71.985 ± 4.858 in blood glucose levels. The ANOVA analysis confirmed that the percentage decrease in blood sugar levels was significantly different from the positive control, indicating a higher effectiveness of the combination treatment in reducing blood glucose levels than the positive control.
本研究探讨了芒果叶(Mangifera foetida L.)和露兜树叶(Pandanus amaryllifolius Roxb.)组合的潜在抗糖尿病活性。芒果叶含有一种名为芒果苷的化合物,具有抗糖尿病和抗氧化作用。另一方面,露兜树叶含有丰富的萜类化合物和类固醇,也具有抗糖尿病作用。为评估抗糖尿病效果,对巴布亚/克品系雄性小鼠进行了口服葡萄糖耐量试验(OGTT)。该试验包括以 1:1 的比例给小鼠服用从 Mangifera foetida L.和 Pandanus amaryllifolius Roxb.中提取的乙醇叶提取物。该研究采用了预试验设计,包括一个对照组和六个治疗组,每个治疗组由四只雄性小鼠组成。各组情况如下第 1 组为正常组,不做任何处理。第二至第四组接受单一提取物或提取物组合,剂量分别为 62.5 毫克/200 克体重、125 毫克/200 克体重和 250 毫克/200 克体重。第五组作为阳性对照,口服葡萄糖和格列本脲,剂量为 0.09 毫克/200 克体重。第六组为阴性对照组,口服葡萄糖和蒸馏水。血糖水平下降百分比最高的一组是按 0.078 克/20 克体重的剂量给小鼠口服葡萄糖,以及按 35 毫克/20 克体重的剂量给小鼠口服芒果叶和露兜树叶提取物(1:1)。该组的血糖水平下降了 71.985 ± 4.858。方差分析证实,血糖水平下降的百分比与阳性对照组有显著差异,表明联合疗法在降低血糖水平方面比阳性对照组更有效。
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引用次数: 0
Genetic diversity of Ulva lactuca from the intertidal zone in Ulee Lheue beach Aceh, Indonesia 印度尼西亚亚齐 Ulee Lheue 海滩潮间带乳莼的遗传多样性
Pub Date : 2024-02-22 DOI: 10.24815/jn.v24i1.33972
Rahmah Hayati, Fawwa Rahly
Ulee Lheue Beach in Banda Aceh was developed for tourism. The macroalgae and seaweed species found in this area have been widespread but never exploited. Among these species is the green seaweed, Ulva lactuca, commonly known as sea lettuce. The physiological variations and genetic features of the seaweed can be attributed to the variations in the coastal environment. This study aims to describe the molecular identity of the genetic diversity of U. lactuca from the intertidal zone at Ulee Lheue Beach. The development of molecular biotechnology has enabled identification the gene expression through genomic DNA to PCR amplification. Genetic distance was determined using UPGMA. The results from 600-bp fragments were analyze the genetic diversity. A total of 15 (31%) expression identified as polymorphic (0.500). Heterozygosity (He) and allelic differential (Na) diversity were found of 1.500–3.000. The highest PIC was observed in the rbcL1, with a correlation between subpopulations of 0.459. PCR amplification using the degenerate primer rbcL1 produced fragments ranging from 300 to 460 bp, whereas the expression of UL2 was detected at 448 and 500 bp using the rbcL2 primer. The phylogenetic identify are shown two (2) clusters. The populations of UL1, UL3, and UL4 were found a close relationship. Furthermore, the UL1 and UL2 populations were further divided into distinct clusters but related to the main branch of UL3. Based on this research, the concern of U. lactuca species for industrial and biotechnology destinations, we can describe a suitable method for obtaining he genetic distances between species.
班达亚齐的 Ulee Lheue 海滩是为旅游业开发的。在这一地区发现的大型藻类和海藻物种分布广泛,但从未被开发过。在这些物种中,有一种绿色海藻--乳莼,俗称海莴苣。海藻的生理变化和遗传特征可归因于沿海环境的变化。本研究旨在描述 Ulee Lheue 海滩潮间带莼菜遗传多样性的分子特征。分子生物技术的发展使得通过基因组 DNA 进行 PCR 扩增鉴定基因表达成为可能。利用 UPGMA 方法确定了遗传距离。对 600-bp 片段的结果进行了遗传多样性分析。共有 15 个(31%)表达被鉴定为多态(0.500)。发现杂合度(He)和等位基因差(Na)多样性为 1.500-3.000。在 rbcL1 中观察到最高的 PIC,亚群之间的相关性为 0.459。使用退化引物 rbcL1 进行 PCR 扩增产生了 300 至 460 bp 的片段,而使用 rbcL2 引物则在 448 和 500 bp 处检测到了 UL2 的表达。系统发育鉴定显示了两(2)个群。发现 UL1、UL3 和 UL4 的种群关系密切。此外,UL1 和 UL2 群体被进一步划分为不同的簇,但与 UL3 的主分支相关。基于这项研究,考虑到工业和生物技术领域对 U. lactuca 物种的关注,我们可以描述一种获取物种间遗传距离的合适方法。
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引用次数: 0
Identification ratio of Si/Ti and Ca/Ti content by X-Ray Fluorescence in tsunami soil samples 利用 X 射线荧光鉴定海啸土壤样本中 Si/Ti 和 Ca/Ti 的含量比
Pub Date : 2024-02-22 DOI: 10.24815/jn.v24i1.35429
K. Lahna, R. Mitaphonna, Muliadi Ramli*, Faliqul Isbah, Elin Yusibani, Fauzi Fauzi, Nazlina Ismail, Nasrullah Idrs
Earthquakes and tsunamis natural disasters have repeatedly occurred on the coast of Aceh province, which lies between the confluence of two plates. The tsunami deposits in this area can provide important information regarding the reconstruction of marine attacks by past earthquakes and tsunamis. In general, tsunami deposits can be identified based on their geological, sedimentological, paleontological, and geochemical characteristics. In our research work, spectrometry X-ray fluorescence (XRF) has been utilized to investigate the geochemical signatures of tsunami-affected soil samples in Aceh province at three tsunami-impacted areas, namely Aceh Besar regency, Banda Aceh City, and Aceh Barat regency. The sampling point is located about a kilometer from the coastal line. Our findings indicate that tsunami-affected soils in Aceh Province after 10 years struck by tsunami contain terrestrial markers such as Fe and Ti, carbonate markers (Mg, Ca), and heavy metals elements (Cr, Ni, Cu, Zn, and Sr). On the other hand, in our study, the concentration ratios of several elements such as Si/Ti and Ca/Ti seem most suitable as a chemical signature for differentiating environmental conditions such as the 2004 Indian Ocean tsunami event. It could be noticed that geochemical analysis by XRF can be applied to characterize the tsunami-affected soils in several coastal areas of Aceh province.
位于两大板块交汇处的亚齐省海岸曾多次发生地震和海啸自然灾害。该地区的海啸沉积物可以为重建过去地震和海啸对海洋的侵袭提供重要信息。一般来说,海啸沉积物可根据其地质学、沉积学、古生物学和地球化学特征进行识别。在我们的研究工作中,利用光谱 X 射线荧光 (XRF) 技术调查了亚齐省三个受海啸影响地区(即亚齐 Besar 县、班达亚齐市和亚齐 Barat 县)受海啸影响土壤样本的地球化学特征。采样点距离海岸线约一公里。我们的研究结果表明,亚齐省受海啸影响 10 年后的土壤中含有陆地标记(如铁和钛)、碳酸盐标记(镁、钙)和重金属元素(铬、镍、铜、锌和锶)。另一方面,在我们的研究中,Si/Ti 和 Ca/Ti 等几种元素的浓度比似乎最适合作为区分 2004 年印度洋海啸事件等环境条件的化学特征。可以注意到,利用 XRF 进行地球化学分析可用于描述亚齐省多个沿海地区受海啸影响土壤的特征。
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引用次数: 0
On the dynamics of spring-pendulum system: an overview of configuration space and phase space 论弹簧摆系统的动力学:构型空间和相空间概述
Pub Date : 2024-02-22 DOI: 10.24815/jn.v24i1.33247
Siti Wahyuni, N. W. Rini, J. Saefan
The dynamics of the spring-pendulum system with two degrees of freedom were studied. The motion of this system is restricted to be in a vertical plane so that the chosen generalized coordinates are the increased length of the spring  and the swing angle of pendulum . Hamiltonian of the system is obtained from the Legendre transformation of Lagrangian. Hamilton’s equation yields four differential equations that represent the dynamic of the system. The obtained results were visualized in configuration space and phase space trajectories. It is shown that generally the greater the initial swing angle, the more complex pattern will occur followed by the appearance of chaotic phenomena.
研究了具有两个自由度的弹簧摆系统的动力学。该系统的运动被限制在垂直平面内,因此选择的广义坐标为弹簧的增大长度和摆锤的摆动角度。根据拉格朗日的 Legendre 变换可得到系统的哈密顿方程。汉密尔顿方程产生了表示系统动态的四个微分方程。得到的结果在构型空间和相空间轨迹中得到直观体现。结果表明,一般情况下,初始摆动角度越大,模式越复杂,随后会出现混沌现象。
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引用次数: 0
Factors affecting the happiness level in Southeast Asia countries with a multilevel approach 用多层次方法分析影响东南亚国家幸福水平的因素
Pub Date : 2024-02-22 DOI: 10.24815/jn.v24i1.36076
Oktiva Rahmawati Dhewi, Kismiantini Kismiantini
The level of the population’s well-being can be measured by using a subjective well-being indicator which is called happiness, which can be affected by various factors from individuals. However, the background of individuals in a group cannot be ignored. Therefore, this study aimed to determine the factors that affect the level of happiness in Southeast Asian countries at the individual and country levels. Data came from World Value Survey (WVS) Wave 7 with 11,387 individuals from the Philippines, Indonesia, Malaysia, Myanmar, Singapore, Thailand, and Vietnam. Data were analyzed using an ordinal response multilevel model with the level of happiness as a response variable. The results showed that the happiness level was affected by gender, marital status, health status, income, religiosity, education level, life satisfaction, and freedom of choice. However, age at the individual level and variables at the country level did not affect the happiness level.
人们的幸福感水平可以通过一个主观幸福感指标来衡量,这个指标被称为幸福感,它可能受到来自个人的各种因素的影响。然而,群体中个人的背景也不容忽视。因此,本研究旨在从个人和国家层面确定影响东南亚国家幸福水平的因素。数据来自世界价值调查(WVS)第 7 波,共有来自菲律宾、印度尼西亚、马来西亚、缅甸、新加坡、泰国和越南的 11,387 人参与。数据分析采用了以幸福水平为响应变量的顺序响应多层次模型。结果显示,幸福程度受性别、婚姻状况、健康状况、收入、宗教信仰、教育水平、生活满意度和选择自由度的影响。然而,个人层面的年龄和国家层面的变量并不影响幸福水平。
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引用次数: 0
Total phenolic and flavonoids content, and antioxidant activity of kratom (Mitragyna speciosa Korth.) leaf ethanol extract 桔梗(Mitragyna speciosa Korth.)叶乙醇提取物的总酚和类黄酮含量以及抗氧化活性
Pub Date : 2024-02-22 DOI: 10.24815/jn.v24i1.33125
M. Masriani, Purnama Melania, R. Muharini, A. Alimuddin, Rody Putra Sartika
This study aims to determine and compare antioxidant activity, total phenolics, flavonoids, and the relationship of phenolic and total flavonoids with the antioxidant activity of the three kratom variants. The ethanol extract of the third variant of kratom leaves was obtained by maceration. Through the antioxidant activity test using DPPH method, all three extracts showed strong antioxidant activity with IC50 values of 26.39, 30.25, and 30.59 μg/mL. The relationship of total phenolics and flavonoids with antioxidant activity was determined by the Pearson correlation test. Examination of total phenolic content using the Folin-Ciocalteu method with successive results for green, red, and white kratom are 6.11, 8.67, and 9.09 mg GAE/g extract. Examination of total flavonoid content using the colorimetric method for green, red, and white kratom were 0.86, 0.68, and 1.13 mg QE/g extract, respectively. The total phenolic content and antioxidant activity showed a correlation coefficient value of -0.32 (P0.05), and the total flavonoid content to antioxidant activity showed a correlation coefficient value of 0.81 (P0.05). Thus, all three variants of kratom have potency as natural antioxidants, but their total phenolic and flavonoid content does not influence their antioxidant activity.
本研究旨在确定和比较三种桔梗变体的抗氧化活性、总酚类、类黄酮以及酚类和总类黄酮与抗氧化活性的关系。第三种变体桔梗叶片的乙醇提取物是通过浸渍法获得的。通过 DPPH 法进行抗氧化活性测试,三种提取物均显示出较强的抗氧化活性,IC50 值分别为 26.39、30.25 和 30.59 μg/mL。总酚和类黄酮与抗氧化活性的关系通过皮尔逊相关性检验来确定。用 Folin-Ciocalteu 法检测总酚含量,绿色、红色和白色桔梗的连续结果分别为 6.11、8.67 和 9.09 毫克 GAE/克提取物。用比色法检测绿色、红色和白色桔梗的总黄酮含量分别为 0.86、0.68 和 1.13 毫克 QE/克提取物。总酚含量与抗氧化活性的相关系数为-0.32(P0.05),总黄酮含量与抗氧化活性的相关系数为0.81(P0.05)。因此,这三种桔梗变种都具有天然抗氧化剂的功效,但它们的总酚类和类黄酮含量并不影响其抗氧化活性。
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引用次数: 0
The difference in leaves production, protein and calcium of Moringa oleifera under modification planting media, application of PGR and nitrogen 在改变种植介质、施用 PGR 和氮的条件下油辣木的叶片产量、蛋白质和钙的差异
Pub Date : 2024-02-22 DOI: 10.24815/jn.v24i1.32403
R. Sulistiani, Mukhtar Yusuf, Syaiful Amri Saragih
Moringa has many ingredients of nutrients that are beneficial for food sources and nutrients that have not been widely cultivated. The nutritional content, benefits and high demand for Moringa abroad will open large opportunities for exporting Moringa flour. Foods full of nutrition will support the maintenance of good public health. For this reason, it is necessary to study and research cultivation techniques that produce high Moringa leaves and can be available sustainably. Production of Moringa leaves as a source of secondary metabolites can be increased by modifying the planting media and applying Plant Growth Regulator (PGR) and Nitrogen. The study used Split Split Plot Design with the main plot immersion by PGR, consisting of 3 types, namely: G1 (Fresh water), G2 (Coconut water), and G3 (GA3). The subplot was the treatment of planting media with two types: M₁ (soil: sand: manure = 1:1:2); M₂ (soil: sand: manure = 1:2:1). The sub subplots were N (urea) fertilizer, with four levels: N0 (0 g/plant); N1 (5 g/plant); N₂ (10 g/plant); and N₃ (15 g/plant). Each treatment combination goes over three times. The agronomic parameters observed were plant height, the number of leaves, fresh crop weight, and root volume, and the biochemical parameters observed were chlorophyll, protein, and calcium levels. The composition of the planting media caused significant differences in plant height at 4, 6, and 10 weeks after planting (WAP), the number of leaves at 4 WAP, and root length at 10 WAP. Growth Regulators significantly affected plant height at 4, 6, and 10 WAP, the number of leaves at 4 WAP, and root length at harvest. Nitrogen fertilization caused significant differences in plant height at 4, 6, and 10 WAP, volume, and root length at harvest (10 WAP). The combination of Planting media, PGR, and Nitrogen treatments caused significant differences in plant height at 4, 6, and 10 WAP and the number of leaves at 6 WAP. Laboratory analysis in this study showed high calcium and protein in Moringa leaves.
辣木含有许多对食物来源有益的营养成分和尚未广泛种植的营养成分。辣木的营养成分、益处和国外对辣木的大量需求将为辣木粉的出口带来巨大商机。富含营养的食品将为维护公众健康提供支持。因此,有必要学习和研究能够生产大量辣木叶并可持续供应的栽培技术。作为次生代谢物来源的辣木叶的产量可以通过改变种植介质和施用植物生长调节剂(PGR)和氮来提高。该研究采用了分割式小区设计,主小区采用 PGR 浸种,包括 3 种类型,即 G1(淡水)、G2(淡水)、G3(淡水)和 G4(淡水):G1(淡水)、G2(椰子水)和 G3(GA3)。子小区为种植介质处理,有两种类型:M₁(土:沙:粪=1:1:2);M₂(土:沙:粪=1:2:1)。子地块为氮(尿素)肥料,有四个水平:N0(0 克/株);N1(5 克/株);N₂(10 克/株);N₃(15 克/株)。每个处理组合重复三次。观察到的农艺参数有株高、叶片数、作物鲜重和根量,观察到的生化参数有叶绿素、蛋白质和钙含量。种植介质的组成对种植后 4 周、6 周和 10 周的株高、4 周的叶片数和 10 周的根长有显著差异。生长调节剂对种植后 4 周、6 周和 10 周的株高、种植后 4 周的叶片数和收获时的根长有明显影响。氮肥对 4、6 和 10 WAP 期的株高、体积和收获时(10 WAP)的根长都有明显影响。种植介质、植物生长激素和氮肥的综合处理会导致 4、6 和 10 WAP 期的株高以及 6 WAP 期的叶片数出现显著差异。本研究的实验室分析表明,辣木叶片中的钙和蛋白质含量较高。
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引用次数: 0
Phytochemical screening and antibacterial activity testing of n-hexane extract from Barringtonia asiatica seeds 巴林藤种子正己烷提取物的植物化学筛选和抗菌活性测试
Pub Date : 2024-02-22 DOI: 10.24815/jn.v24i1.32597
Nurhaida Nurhaida, M. Murniana, Jainursumarmi Safrullah Atanta
Barringtonia asiatica, a marine plant belonging to the Lecythidaceae family, exhibits bioactivity in all parts of the plant. In the Simeulue region, the seeds of this plant are used for fish trapping. The polar extracts of B. asiatica seeds has shown antibacterial activity, but the non-polar extracts has not been studied yet. Therefore, this study aimed to determine the secondary metabolites of B. asiatica using a non-polar solvent (n-hexane) and evaluate its activity against Gram-positive and Gram-negative bacteria. The extraction method employed using soxhlet extraction, and the antibacterial activity was determined using the Kirby-Bauer method. The presence of secondary metabolites in the B. asiatica seed extract was assessed using phytochemical tests, and its chemical composition was analyzed using Gas Chromatography Mass Spectrophotometry (GC-MS). The yield of n-hexane extract from B. asiatica seeds was 1.7%, and the phytochemical analysis revealed that the B. asiatica seed’s n-hexane extract contains only terpenoids compounds. The B. asistica seed’s n-hexane extract at concentration of 10% (w/v) showed the highest zone of inhibition against Staphylococcus aureus (9.3 mm) followed by Escherichia coli (9,9 mm). The GC-MS analysis showed that B. asiatica seed’s n-hexane extract contains Guaiene, Guaiene, Ledene, 9,12-Octadecadienoic acid, and Ethyl linoleate. Based on these findings, it can be concluded that B. asiatica seed's n-hexane extract exhibited antibacterial activity against S. aureus and E. coli.
Barringtonia asiatica 是一种海洋植物,属于茜草科,植物的各个部分都具有生物活性。在 Simeulue 地区,这种植物的种子被用于诱捕鱼类。茜草种子的极性提取物具有抗菌活性,但对非极性提取物尚未进行研究。因此,本研究旨在使用非极性溶剂(正己烷)确定茜草的次生代谢物,并评估其对革兰氏阳性和革兰氏阴性细菌的活性。提取方法采用索氏提取法,抗菌活性采用柯比-鲍尔法测定。利用植物化学测试评估了茜草种子提取物中次生代谢物的存在,并利用气相色谱质谱联用仪(GC-MS)分析了其化学成分。茜草种子正己烷提取物的产量为 1.7%,植物化学分析显示茜草种子的正己烷提取物只含有萜类化合物。在 10%(w/v)的浓度下,白花蛇舌草种子正己烷提取物对金黄色葡萄球菌的抑制面积最大(9.3 毫米),其次是大肠杆菌(9.9 毫米)。气相色谱-质谱分析表明,白花蛇舌草种子的正己烷提取物中含有愈创木烯、愈创木烯、褐煤烯、9,12-十八碳二烯酸和亚油酸乙酯。根据这些发现,可以得出结论:白千层种子的正己烷提取物对金黄色葡萄球菌和大肠杆菌具有抗菌活性。
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