Pub Date : 2024-07-21DOI: 10.31857/s0555109924010121
O. S. Kuprienko, I. Vashkevich, A. I. Zilberman, O. Sviridov
The influence of thermodynamic and kinetic conditions on the interaction of polyclonal antibodies to penicillins with the antibiotics of a penicillin group was studied in the system of a direct enzyme-linked immunosorbent assay (ELISA). Minimum differences in the cross reactions of the polyclonal antibodies with different penicillins were observed when the ELISA was carried out at 4°C for 1 hour. An increase in temperature and duration of the assay led to an increase in antibodies reactivity only to amoxicillin, and significantly enhanced differences among the sensitivities of individual penicillins determination. Under the chosen assay conditions, the following antibodies cross-reactivity values were obtained: to penicillin G — 90%, to ampicillin — 100%, to amoxicillin — 110%. The analytical sensitivity was 0.03 ng/mL for ampicillin, and the limit of ampicillin quantification in milk was 0.4 μg/L. The developed group-specific ELISA was used for the determination in milk of seven penicillins that are regulatory controlled in foods and raw materials of animal origin — penicillin G, ampicillin, amoxicillin, cloxacillin, oxacillin, dicloxacillin and nafcillin.
{"title":"Dependence of the Group Specificity of Immunoenzyme Determination of Penicillins in Milk on the Temperature and Duration of Antibiotic Cross Reactions with Polyclonal Antibodies","authors":"O. S. Kuprienko, I. Vashkevich, A. I. Zilberman, O. Sviridov","doi":"10.31857/s0555109924010121","DOIUrl":"https://doi.org/10.31857/s0555109924010121","url":null,"abstract":"The influence of thermodynamic and kinetic conditions on the interaction of polyclonal antibodies to penicillins with the antibiotics of a penicillin group was studied in the system of a direct enzyme-linked immunosorbent assay (ELISA). Minimum differences in the cross reactions of the polyclonal antibodies with different penicillins were observed when the ELISA was carried out at 4°C for 1 hour. An increase in temperature and duration of the assay led to an increase in antibodies reactivity only to amoxicillin, and significantly enhanced differences among the sensitivities of individual penicillins determination. Under the chosen assay conditions, the following antibodies cross-reactivity values were obtained: to penicillin G — 90%, to ampicillin — 100%, to amoxicillin — 110%. The analytical sensitivity was 0.03 ng/mL for ampicillin, and the limit of ampicillin quantification in milk was 0.4 μg/L. The developed group-specific ELISA was used for the determination in milk of seven penicillins that are regulatory controlled in foods and raw materials of animal origin — penicillin G, ampicillin, amoxicillin, cloxacillin, oxacillin, dicloxacillin and nafcillin.","PeriodicalId":335560,"journal":{"name":"Прикладная биохимия и микробиология","volume":"16 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141818471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1134/s0555109919010094
Д. С. Копицын, М. С. Котелев, М. Ю. Зиангирова, П. А. Гущин, А. А. Новиков
{"title":"Применение пероксидной обработки для идентификации бактерий по спектрам гигантского комбинационного рассеяния","authors":"Д. С. Копицын, М. С. Котелев, М. Ю. Зиангирова, П. А. Гущин, А. А. Новиков","doi":"10.1134/s0555109919010094","DOIUrl":"https://doi.org/10.1134/s0555109919010094","url":null,"abstract":"","PeriodicalId":335560,"journal":{"name":"Прикладная биохимия и микробиология","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126596335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1134/s0555109919050118
М. В. Семёнова, А. М. Рожкова, Д. О. Осипов, А. Д. Сатрутдинов, О. А. Синицына, Е. А. Рубцова, Е. Г. Кондратьева, А. П. Синицын
{"title":"Подбор оптимального комплекса ферментов для гидролиза углеводов свекловичного жома","authors":"М. В. Семёнова, А. М. Рожкова, Д. О. Осипов, А. Д. Сатрутдинов, О. А. Синицына, Е. А. Рубцова, Е. Г. Кондратьева, А. П. Синицын","doi":"10.1134/s0555109919050118","DOIUrl":"https://doi.org/10.1134/s0555109919050118","url":null,"abstract":"","PeriodicalId":335560,"journal":{"name":"Прикладная биохимия и микробиология","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121056525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31857/s0555109922010044
О. В. Космачевская, Э. И. Насыбуллина, А. Ф. Топунов
{"title":"Пероксидазная активность легоглобина клубеньков бобов (\u0000 \u0000 Vicia faba\u0000 \u0000 L.) по отношению к гидропероксиду\u0000 \u0000 трет-\u0000 \u0000 бутила","authors":"О. В. Космачевская, Э. И. Насыбуллина, А. Ф. Топунов","doi":"10.31857/s0555109922010044","DOIUrl":"https://doi.org/10.31857/s0555109922010044","url":null,"abstract":"","PeriodicalId":335560,"journal":{"name":"Прикладная биохимия и микробиология","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116526864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1134/S0555109919020168
Н. В. Жарикова, Е. Ю. Журенко, Т. Р. Ясаков, Валентин Викторович Коробов, А. С. Ерастов, Т. В. Маркушева
{"title":"Особенности конверсии 4-хлорфеноксиуксусной кислоты у штамма\u0000 \u0000 Pseudomonas\u0000 \u0000 sp. 36DCP","authors":"Н. В. Жарикова, Е. Ю. Журенко, Т. Р. Ясаков, Валентин Викторович Коробов, А. С. Ерастов, Т. В. Маркушева","doi":"10.1134/S0555109919020168","DOIUrl":"https://doi.org/10.1134/S0555109919020168","url":null,"abstract":"","PeriodicalId":335560,"journal":{"name":"Прикладная биохимия и микробиология","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117016786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31857/s0555109920060112
Э. Р. Михеева, И. В. Катраева, Д. Л. Ворожцов, Ю. В. Литти, А. Н. Ножевникова
{"title":"Эффективность двухфазного анаэробного сбраживания и физико-химические свойства органической фракции твердых коммунальных отходов, предобработанных в аппарате вихревого слоя","authors":"Э. Р. Михеева, И. В. Катраева, Д. Л. Ворожцов, Ю. В. Литти, А. Н. Ножевникова","doi":"10.31857/s0555109920060112","DOIUrl":"https://doi.org/10.31857/s0555109920060112","url":null,"abstract":"","PeriodicalId":335560,"journal":{"name":"Прикладная биохимия и микробиология","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129426137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31857/s0555109922020076
Е. А. Гильванова, Г. Э. Актуганов, В. Р. Сафина, П. Ю. Мильман, Сергей Александрович Лопатин, А. И. Мелентьев, Н. Ф. Галимзянова, Л. Ю. Кузьмина, Т. Ф. Бойко
{"title":"Характеристика термотолерантной хитиназы штамма\u0000 \u0000 Cohnella\u0000 \u0000 sp. IB P-192 и ее применение для получения биоактивных олигомеров хитозана","authors":"Е. А. Гильванова, Г. Э. Актуганов, В. Р. Сафина, П. Ю. Мильман, Сергей Александрович Лопатин, А. И. Мелентьев, Н. Ф. Галимзянова, Л. Ю. Кузьмина, Т. Ф. Бойко","doi":"10.31857/s0555109922020076","DOIUrl":"https://doi.org/10.31857/s0555109922020076","url":null,"abstract":"","PeriodicalId":335560,"journal":{"name":"Прикладная биохимия и микробиология","volume":"229 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121303447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1134/s0555109919050027
А. Н. Дойникова, Н. Л. Векшин
{"title":"Детекция гидролиза РНК биназой по флуоресценции акридинового оранжевого","authors":"А. Н. Дойникова, Н. Л. Векшин","doi":"10.1134/s0555109919050027","DOIUrl":"https://doi.org/10.1134/s0555109919050027","url":null,"abstract":"","PeriodicalId":335560,"journal":{"name":"Прикладная биохимия и микробиология","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124140614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31857/s055510992105007x
И. А. Гаврюшина, М. Л. Георгиева, А.Е. Куварина, В. С. Садыкова
{"title":"Пептаиболы как потенциальные антифунгальные и противоопухолевые антибиотики: современные исследования и перспективы (обзор)","authors":"И. А. Гаврюшина, М. Л. Георгиева, А.Е. Куварина, В. С. Садыкова","doi":"10.31857/s055510992105007x","DOIUrl":"https://doi.org/10.31857/s055510992105007x","url":null,"abstract":"","PeriodicalId":335560,"journal":{"name":"Прикладная биохимия и микробиология","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127753923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.31857/s0555109922020118
М. В. Коновалова, Д. С. Царегородцева, А. Н. Венжик, Р. А. Полтавцева, Елена Викторовна Свирщевская
{"title":"Противоспаечный эффект материалов на основе карбоксиметилхитозана и карбоксиметилцеллюлозы","authors":"М. В. Коновалова, Д. С. Царегородцева, А. Н. Венжик, Р. А. Полтавцева, Елена Викторовна Свирщевская","doi":"10.31857/s0555109922020118","DOIUrl":"https://doi.org/10.31857/s0555109922020118","url":null,"abstract":"","PeriodicalId":335560,"journal":{"name":"Прикладная биохимия и микробиология","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131793406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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