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Development of Rapid Vigor Test Using Urine Sugar Analysis Paper for Soybean (Glycine max L.) Seeds 大豆(Glycine max L.)尿糖分析纸快速活力试验的研制种子
Pub Date : 2022-02-09 DOI: 10.29244/jtcs.9.01.22-30
Jean D’amour Rukundo, M. R. Suhartanto, S. Ilyas
Seed vigor is an essential parameter of seed quality. It plays a critical value in the decision-making for the seeds to be used in agronomic and horticultural crops. The urinary sugar analysis paper (USAP) test determines seed vigor based on leakage concentration from the seed and corresponding color change of the USAP, which are obtained after soaking the seeds in water.  This research was conducted from January 2021 to November 2021, intending to develop a rapid soybean vigor test using USAP, and it was composed of two experiments. Soybean seeds of the Biosoy1 variety were obtained from ICABIOGRAD (Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development). The seeds consisted of three different levels of seed lots (low, medium, and high viability with 35%, 64%, and 94% germination percentages, respectively). The first experiment was to determine moisture content and soaking period for the USAP seed vigor test. It was arranged in a completely randomized design with one factor as the combination of three seed lots, two levels of seed moisture content (10-12% and 13-14%), and four levels of the soaking period (0, 6, 8, and 10 hours). The second experiment was the optimization of the soaking period by seed number. It was arranged in a completely randomized design with one factor, which was combinations of three seed lots, three levels of seed numbers (50, 75, and 100 seeds), and the soaking period (four levels as 0, 6, 8, and 10 hours). The data were analyzed statistically by the Minitab package. The result showed that both combinations of 10-12% and 13-14% moisture content with the soaking period of 10 hours effectively differentiated the three seed lots into three vigor levels using USAP. Experiment 2 optimized the soaking period from 10 hours to 8 hours by using 100 seeds. The USAP color change was effectively significant to indicate seed lot vigor levels, mainly based on protein leakage concentration on USAP. The color changes from pale yellow-green for higher vigor seed lot to light greenish-blue for low vigor. The USAP was effective for the soybean vigor test for the seed lot with 10-14% moisture content by soaking 100 seeds into 50 ml of distilled water for 8 hours. The USAP is a promising rapid vigor test method but still needs to be developed further.
种子活力是衡量种子品质的重要指标。它对农艺和园艺作物种子的使用决策具有重要价值。尿糖分析纸(USAP)测试根据种子在水中浸泡后的泄漏浓度和USAP的相应颜色变化来确定种子活力。本研究于2021年1月至2021年11月进行,旨在利用USAP开发大豆活力快速试验,由两个试验组成。Biosoy1品种的大豆种子来自印度尼西亚农业生物技术和遗传资源研究与开发中心。种子由低、中、高三个不同水平的种子批次组成,发芽率分别为35%、64%和94%。第一个试验是测定USAP种子活力试验的水分含量和浸泡时间。采用完全随机设计,1因素组合为3个种子批次,2个种子含水率水平(10-12%和13-14%),4个浸泡时间水平(0、6、8和10小时)。第二个试验是通过种子数量对浸渍期进行优化。采用单因素完全随机设计,即3个种子批次、种子数量3个水平(50、75、100粒)和浸泡时间(0、6、8、10小时4个水平)的组合。采用Minitab软件包对数据进行统计分析。结果表明,10-12%和13-14%水分浸泡10 h的组合均能有效地将3个种子区划分为3个活力等级。试验2将100粒种子浸泡时间从10小时优化到8小时。USAP颜色变化对种子活力水平有显著的指示作用,主要基于USAP上的蛋白质泄漏浓度。颜色从黄绿色的高活力种子到浅绿蓝色的低活力种子。USAP对水分含量为10-14%的大豆种子进行活力试验,将100粒种子浸泡在50 ml蒸馏水中8小时。USAP是一种很有前途的快速活力测试方法,但仍需进一步发展。
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引用次数: 0
Investigation of Vibration Technique To Control Physical Properties of Yam Tubers (Dioscorea rotundata Poir) uring Storage 振动技术控制山药块茎贮藏过程中物理特性的研究
Pub Date : 2022-02-09 DOI: 10.29244/jtcs.9.01.77-85
Kifilideen L. Osanyinpeju, A. Aderinlewo, O. Dairo, O. Adetunji, E. Ajisegiri
Yam tubers lose weight during storage and prolonged storage can reduce tuber quality and quantity. This study investigated the application of vibration techniques for the control of the physical properties of yam tubers during storage. Measurements were conducted on the physical properties of the tubers: weight loss, shrinkage of the middle diameter, shrinkage of the length, top and bottom diameter, on 108 tubers treated and 32 tubers untreated (control). The factors of the experimental design were three levels of vibration frequencies, i.e. low (1 – 5 Hz), medium (60 – 100 Hz) and high (150 – 200 Hz), amplitudes of low (5 mm), medium (10 mm) and high (20 mm), and durations of low (5 minutes), medium (10 minutes) and high (15 minutes). Tuber weights were classified as small (0.1 – 2.9 kg) and large (3.0 – 5.0 kg). The tubers were stored for ten weeks after vibration, and their physical properties were measured every week during the storage period. Our study demonstrated that as the frequency, amplitude, and duration of vibration increase, the physical properties of yam tubers decrease significantly in both tuber classes. The study shows that mechanical vibration can slow down the changes in the physical properties of the yam tuber during storage.
山药块茎在贮藏过程中会发生重量下降,贮藏时间过长会使块茎的质量和数量下降。研究了振动技术在薯蓣块茎贮藏过程中物理特性控制中的应用。对108个处理过的块茎和32个未处理过的块茎(对照)进行了物理性能的测量:失重、中径收缩、长度、顶径和底径收缩。实验设计的因素为低(1 - 5hz)、中(60 - 100hz)和高(150 - 200hz)三个振动频率水平,低(5 mm)、中(10 mm)和高(20 mm)幅值,低(5分钟)、中(10分钟)和高(15分钟)持续时间。块茎重量分为小块茎(0.1 - 2.9 kg)和大块茎(3.0 - 5.0 kg)。振动后的块茎贮存10周,贮存期间每周测定块茎的物理性质。我们的研究表明,随着振动频率、振幅和持续时间的增加,薯类块茎的物理特性显著降低。研究表明,机械振动能减缓薯蓣块茎贮藏过程中物理特性的变化。
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引用次数: 0
Comparing the Genetic Parameters of Three Rice Varieties on Suboptimal Land Using the SRI Method 利用SRI方法比较三个水稻品种在次优土地上的遗传参数
Pub Date : 2022-02-09 DOI: 10.29244/jtcs.9.01.47-51
N. Rozen, M. Kasim, I. Dwipa, D. Hervani
The conversion of fertile land into industrial areas and construction development has contributed to the decline of rice production in Indonesia. Therefore, it is necessary to expand the rice fields to the sub-optimal land, i.e. lands with high levels of Fe and Al and low soil pH. One of the solutions that can potentially solve the problem is choosing varieties of rice that can adapt to the suboptimal land environment; the rice varieties can be obtained by estimating rice genetic parameters and selecting the optimal planting methods. One method that can potentially improve rice yield is “System of Rice Intensification”, or SRI method. Our study was conducted in the rice fields on suboptimal land at Pasar Ambacang, Kuranji District, Padang, Sumatra, from December 2019 to April 2020.  The aims of the experiment are to determine the genetic parameters and responses of the three rice varieties to suboptimal land using the SRI method. A complete randomized design with four replications was used to set up the experiment using three varieties of rice, i.e. “Batang Piaman”, “Bujang Marantau”, and “IPB3S”. Based on the plant growth measurement and analysis of the genetic parameters, we found that the plant growth characters, particularly height and panicles length, had high heritability and genetic progression values.
将肥沃的土地转为工业区和建设发展,造成了印度尼西亚稻米产量的下降。因此,有必要将稻田扩展到次优土地,即铁、铝含量高、土壤ph值低的土地。选择适应次优土地环境的水稻品种是解决这一问题的潜在途径之一;通过对水稻遗传参数的估计,选择最优的种植方法,获得水稻品种。一种可能提高水稻产量的方法是“水稻集约化系统”,即SRI方法。我们的研究于2019年12月至2020年4月在苏门答腊岛巴东Kuranji区Pasar Ambacang次优土地上的稻田进行。试验的目的是利用SRI方法确定三个水稻品种对次优土地的遗传参数和响应。试验采用4个重复的完全随机设计,选用“巴塘皮曼”、“布江马兰头”和“IPB3S”3个水稻品种。通过植物生长测量和遗传参数分析,发现植物生长性状,特别是株高和穗长具有较高的遗传力和遗传进阶值。
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引用次数: 0
A Meta-Analysis of Quantitative Trait Loci Associated with Stay-Green in Sorghum 高粱偏绿相关数量性状位点的meta分析
Pub Date : 2022-02-09 DOI: 10.29244/jtcs.9.01.39-46
Ahmed Aquib, Shadma Nafis
A strong association between stay-green and post-flowering drought tolerance in sorghum has been established previously. In this study, a meta-analysis was conducted to find relevant genetic intervals that regulate stay-green in sorghum and thereby affect drought resilience. Meta-QTL analysis was performed to combine 115 QTLs mapped in previous studies and locate regions where recurrent QTLs occurred across experiments. This approach overcomes the constraints of a single QTL association experiment by increasing heterogeneity in populations and environments. The analysis included the construction of consensus maps, QTL projection, and QTL clustering. We report 32 Meta-QTL for stay-green and their position relative to genetic markers on the sorghum genome. The results from this study will facilitate future attempts aiming to improve and understand drought tolerance in Sorghum by the use of fine mapping and marker-assisted selection.
以前已经建立了高粱保持绿色和开花后抗旱性之间的强烈联系。本研究通过荟萃分析,寻找调控高粱保持绿色从而影响抗旱能力的相关遗传区间。Meta-QTL分析结合了先前研究中绘制的115个qtl,并定位了在实验中出现重复qtl的区域。这种方法通过增加种群和环境的异质性,克服了单一QTL关联实验的局限性。分析包括构建共识图、QTL投影和QTL聚类。我们报道了32个保持绿色的Meta-QTL及其相对于高粱基因组遗传标记的位置。这项研究的结果将有助于未来利用精细作图和标记辅助选择来提高和了解高粱的耐旱性。
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引用次数: 3
Fruit Quality of Guava (Psidium guajava ‘Kristal’) under Different Fruit Bagging Treatments and Altitudes of Growing Location 不同套袋处理和产地海拔对番石榴果实品质的影响
Pub Date : 2022-02-09 DOI: 10.29244/jtcs.9.01.8-14
R. Widyastuti, Rahmat Budiarto, K. Hendarto, H. A. Warganegara, I. Listiana, Yoyon Haryanto Haryanto, H. Yanfika
Fruit quality is an important aspect that requires attention and more study when it comes to commercializing tropical fruits, including guava ‘Kristal’. This study aimed to evaluate the effect of bagging treatment and altitude of growing orchard on fruit quality of guava (Psidium guajava var. ‘Kristal’). This study was conducted at two local orchards managed by small-scale farmers, at Gunung Batu (1000 meters above sea level, m asl) and Brajaselebah (25 m asl), Lampung Province, Indonesia. A nested design was applied to test 5 types of bagging treatments and 2 levels of land altitude. Fruit quality was assessed by measuring both physical and chemical characteristics. Fruit size indicated by fruit weight and diameter was significantly larger in lowland compared to highland. The total soluble solids (TSS) were significantly higher in the lowland compared to the highland, while titratable acid (TA) showed an opposite result. Vitamin C of the fruits varied from140 to 146 mg per 100 g.  Different types of fruit bagging and land altitudes did not affect the physical and chemical characteristics of “Kristal” guava fruits. Fruit scar intensity was higher in lowland, especially in fruit without bagging. Fruit bagging is highly recommended for farmers due to the success of this treatment to reduce fruit scar intensity, thus resulting in good fruit quality, irrespective of the growing locations.
水果质量是热带水果商业化的一个重要方面,需要关注和更多的研究,包括番石榴“水晶”。本研究旨在评价套袋处理和果园种植海拔对番石榴果实品质的影响。这项研究是在印度尼西亚楠榜省的Gunung Batu(海拔1000米)和Brajaselebah(海拔25米)两个由小农管理的当地果园进行的。采用巢式设计,试验5种套袋处理和2个海拔水平。通过测定果实的物理和化学特性来评价果实的品质。以果实质量和直径表示的果实大小在低地显著大于高地。总可溶性固形物(TSS)在低地显著高于高原,而可滴定酸(TA)则相反。每100克水果的维生素C含量从140毫克到146毫克不等。不同套袋方式和海拔对“水晶”番石榴果实的理化特性没有影响。果实疤痕强度在低地较高,特别是未套袋的果实。水果套袋是强烈推荐给农民的,因为这种处理成功地减少了果实疤痕强度,从而产生了良好的果实质量,无论生长地点。
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引用次数: 4
Effectiveness of Various Foliar Fertilizer on the Growth and Performance of Oil Palm (Elaeis guineensis Jacq.) Seedlings in Main Nurseries 不同叶面肥对油棕生长和生产性能的影响主苗圃的幼苗
Pub Date : 2022-02-09 DOI: 10.29244/jtcs.9.01.1-7
Y. Sukmawan, D. Riniarti
Fertilizer can be applied through soil and leaves. Fertilizer application through leaves is more effective than soil application due to faster nutrient absorption. This study aims to determine the effectiveness of applying various foliar fertilizer compositions on the growth and performance of oil palm seedlings. The research was conducted from April 2020 to September 2020 at the Oil Palm Nursery Unit at Politeknik Negeri Lampung. A single factor in a completely randomized design with four replications was used in the experiment. The treatment involved the nutrient composition of foliar fertilizer consisting of five levels, namely control (no fertilizer), NPK 20-15-15, NPK 27-18-9, NPK 11-8-6, and NPK 27.5-5.5-4.8. Measurements were made on seedling height, stem diameter, number of leaves, leaf greenness, rachis length, and leaflet length. The data were analyzed by means of variance, followed by orthogonal contrast if the result was significantly different. The results showed that the application of foliar fertilizers could increase the growth of seedling height, stem diameter, number of leaves, leaf greenness index, rachis length, and leaflet length. Generally, a foliar fertilizer application gives better results than without a foliar fertilizer application (control). There was no difference in the powder and liquid foliar fertilizer effect on increasing the growth of oil palm seedlings. The formulation of NPK 20-15-15 and NPK 11-8-6 foliar fertilizer had a better effect on the leaf greenness index of oil palm seedlings.
肥料可以通过土壤和树叶施用。通过叶片施肥比土壤施肥更有效,因为叶片吸收养分更快。本研究旨在确定施用不同叶面肥料成分对油棕幼苗生长和性能的影响。该研究于2020年4月至2020年9月在Politeknik Negeri Lampung的油棕苗圃进行。试验采用单因素完全随机设计,共4个重复。叶面肥的营养组成包括5个水平,即对照(不施肥)、NPK 20-15-15、NPK 27-18-9、NPK 11-8-6和NPK 27.5-5.5-4.8。测定了幼苗高、茎粗、叶片数、叶片绿度、轴长和小叶长。采用方差法对数据进行分析,差异显著者进行正交对比。结果表明,施叶面肥能促进幼苗高、茎粗、叶片数、叶片绿度指数、轴长和小叶长生长。一般来说,叶面施用肥料比不施用叶面肥料(对照)效果更好。粉剂叶面肥与液体叶面肥对油棕幼苗生长的促进作用无显著差异。NPK 20-15-15和NPK 11-8-6叶面肥配制对油棕幼苗叶片绿度指数有较好的影响。
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引用次数: 0
The Effects of Preharvest Mild Shading on the Quality and Production of Essential Oil from Kaffir Lime Leaves (Citrus hystrix) 采前轻度遮荫对青柠叶精油品质及产量的影响
Pub Date : 2022-02-09 DOI: 10.29244/jtcs.9.01.15-21
Rahmat Budiarto, R. Poerwanto, E. Santosa, D. Efendi, A. Agusta
Kaffir lime (Citrus hystrix DC) is a less popular citrus species commonly used as a food spice and a source of essential oil. Early studies report the success of preharvest mild shading to increase leaf yield, although there is still limited information on the effect of preharvest shading on the quality of essential oil produced. The aim of this current study is to evaluate the effect of preharvest mild shading factors on the yield, physical characteristics, and metabolite fingerprinting of kaffir lime leaves essential oil (KLLEO). One-year-old kaffir lime trees were sampled in two preharvest treatments, i.e., open sun and mild shading (24% light reduction) at Pasir Kuda experimental field, Bogor, Indonesia. Statistical analysis showed that there was no significant effect of preharvest treatment on yield and physical characteristics (color, specific gravity, and refractive index) of KLLEO. In contrast, there was a metabolite fingerprinting variation of KLLEO as an effect of mild shading. The relative percentage of bergamol, citronellol, caryophyllene oxide, citronellic acid, isopulegol, isopulegyl formate, limonene, linalool, and linalool oxide was increased by mild shading. On the other hand, the main metabolite (citronellal) was significantly reduced by about 10% in shading treatment, as compared to the open-sun ones.
卡菲尔酸橙(Citrus hystrix DC)是一种不太受欢迎的柑橘类植物,通常用作食品香料和精油的来源。早期的研究报道了收获前轻度遮荫提高叶片产量的成功,尽管收获前遮荫对所生产的精油质量的影响的信息仍然有限。本研究旨在评价采前轻度遮荫因子对青柠叶精油(KLLEO)产量、物理特性和代谢物指纹图谱的影响。在印度尼西亚茂物的Pasir Kuda试验田,对1年生kaffir酸橙树进行了两种采收前处理,即露天日光和轻度遮阳(光照减少24%)。统计分析表明,采前处理对KLLEO的产量和物理特性(颜色、比重、折射率)无显著影响。相比之下,轻度遮荫对KLLEO的代谢物指纹图谱有影响。佛手柑酚、香茅醇、氧化石竹烯、香茅酸、异丙烯醇、甲酸异丙烯酯、柠檬烯、芳樟醇和氧化芳樟醇的相对百分比在轻度遮荫下均有所增加。另一方面,遮荫处理的主要代谢物香茅醛(citronellal)较光照处理显著降低约10%。
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引用次数: 5
Forage Potential of Plant Species Found in Various Ecosystems in Musi Banyuasin Regency, South Sumatera, Indonesia 印度尼西亚南苏门答腊Musi Banyuasin Regency不同生态系统中发现的植物的饲料潜力
Pub Date : 2022-02-09 DOI: 10.29244/jtcs.9.01.68-76
P. I. Kanny, M. Chozin, E. Santosa, D. Guntoro, S. Zaman, S. Suwarto, A. Kurniawati
Forage is an important component in sustainable smallholder livestock. The purpose of the study was to identify the diversity of natural plant species in various ecosystems, as well as their potential as forage. Based on our surveys of palm oil plantation, rubber plantation, and home gardens using the quadrant method, we identified approximately 50 species comprising the natural vegetation of our study area. Out of 50, we identified 39 broad-leaved species, 9 grasses (Poaceae) species, and 2 sedges (Cyperaceae) species. The palm oil plantation had 29 species dominated by Ottochloa nodosa (11.92%) and Asystasia gangetica (11.40%); 25 species were found in the rubber plantation dominated by Cynodon dactylon (28.42%) and Panicum repens (9.20%), and home gardens contained 30 species dominated by Eleusine indica (13.39%) and Ageratum conyzoides (9.60%). Among the 29 species found in the palm oil plantation, 13 were observed to have low palatability scores, and 16 species have high palatability scores. Based on their high palatability scores, the following species can be utilized as forage for Balinese cattle: Cyrtococcum acrescens, Eleusine indica, Centrosema pubescens, Paspalum conjugatum, and Sida rhombifolia.
饲料是可持续小农畜牧业的重要组成部分。该研究的目的是确定不同生态系统中天然植物物种的多样性,以及它们作为饲料的潜力。在对棕榈油种植园、橡胶种植园和家庭花园进行调查的基础上,采用象限法确定了组成研究区自然植被的约50种植物。其中,阔叶植物39种,禾本科植物9种,莎草科植物2种。棕榈油人工林共有29种,主要种类为毛藻(Ottochloa nodosa)(11.92%)和gangetica (11.40%);橡胶林中以长尾草(28.42%)和白穗草(9.20%)为优势种25种,家庭园林中以青叶草(13.39%)和长尾草(9.60%)为优势种30种。在棕榈油种植园发现的29种植物中,13种具有低适口性,16种具有高适口性。基于它们的高适口性得分,以下物种可以用作巴厘牛的饲料:Cyrtococcum acrescens, Eleusine indica, Centrosema pubescens, Paspalum conjugatum和Sida rhombiolia。
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引用次数: 1
Morphology and Starch Production Potential of Sago Palm Found in Village Haripau, East Mimika Subdistrict, Mimika, Papua Province, Indonesia 印度尼西亚巴布亚省米米卡东米米卡街道Haripau村发现的西米棕榈形态和淀粉生产潜力
Pub Date : 2022-02-09 DOI: 10.29244/jtcs.9.01.31-38
Muhammad Iqbal Nurulhaq, M. H. Bintoro, Supijatno Supijatno
Papua, Indonesia is the largest sago palm (Metroxylon spp) distribution area in the world. Most (85%) of the total sago area in the world is in Indonesia, and 95% of the sago area in Indonesia is in Papua and West Papua. Field observation of accessions of sago palm was conducted at the sago growing area at Hiripau village. The aim of the study was to determine the diversity of sago palm accessions from Hiripau village and compare their morphology and starch production. Based on interviews with the local farmers, the four accessions of sago palm that dominated the Hiripau area are Nakowai, Mapartaro, Tuhai, and Korearipi. These accessions vary in the spine characteristics, features of the trunk, leaf, pith, and starch production. Sago Mapartaro leaves are the largest (23.56 m2) and the leaflet areas are almost twice the size of the other accessions. Tuhai has the highest starch yield but it has high ash content resulting in low starch quality.
印度尼西亚的巴布亚是世界上最大的西米棕榈(Metroxylon spp)分布区。世界上大部分(85%)的西米面积在印度尼西亚,印度尼西亚95%的西米面积在巴布亚和西巴布亚。在Hiripau村西米种植区进行了西米棕的田间观察。本研究的目的是确定Hiripau村西米棕榈资源的多样性,并比较它们的形态和淀粉产量。根据对当地农民的采访,在Hiripau地区占主导地位的四种西米棕榈是Nakowai, Mapartaro, Tuhai和Korearipi。这些植物在脊柱特征、树干特征、叶片、髓和淀粉产量方面各不相同。西米芋头叶片最大(23.56 m2),小叶面积几乎是其他品种的两倍。土海淀粉得率最高,但灰分含量高,淀粉品质不高。
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引用次数: 2
Occurrence and Distribution of Viruses Associated with Okra and Their Alternative Hosts in Kaduna and Zamfara States, Nigeria. 与秋葵及其替代宿主相关的病毒在尼日利亚卡杜纳州和扎姆法拉州的发生和分布
Pub Date : 2021-10-20 DOI: 10.29244/jtcs.8.03.177-186
Gilima ZaharaddeenSamaila, David Kashina Boniface, Olalekan Oyeleke Banwo, Alegbejo Mathew Dada, Charles Chindo Agart, I. Tijjani
One of the major constraints to production of okra (Abelmoschus esculentus L.) in Nigeria and in particular in Kaduna and Zamfara States, is the problem of okra mosaic virus and okra leaf curl virus. This study was carried out to provide information on the occurrence and distribution of okra mosaic and okra leaf curl viruses on okra, in Kaduna and Zamfara states, Nigeria. A survey of okra-producing farms was carried out during dry and wet seasons of 2017 cropping season in Kaduna (Zaria, Lere, and Igabi Local Government Areas) and Zamfara (Gusau, Bungudu, and Zurmi LGAs) states. Leaf samples (15) of symptomatic okra plants were collected from each farm in the study area. The total number of plants and the number of symptomatic plants within each subplot were recorded, and the disease incidence was determined. Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS- ELISA) kit was used in the detection of Okra Mosaic Virus while Polymerase Chain Reaction (PCR) was employed for the detection Okra Leaf Curl Virus. The results showed that all the okra leaf samples tested for OLCV were negative in this study while OkMV was tested positve in all the samples with a recorded incidence of  20 % and 14 %  in Kaduna and Zamfara states respectively, however, only 8 out of total weed samples were also tested positive for OKV, but all were tested negative to OLCV.
在尼日利亚,特别是在卡杜纳州和扎姆法拉州,秋葵花叶病毒和秋葵卷叶病毒是秋葵生产的主要制约因素之一。本研究旨在为尼日利亚卡杜纳州和扎姆法拉州秋葵花叶病毒和秋葵卷叶病毒的发生和分布提供信息。在卡杜纳州(扎里亚、莱尔和伊加比地方政府区)和扎姆法拉州(古索、本古杜和祖尔米地方政府区)2017年种植季的旱季和雨季对秋葵生产农场进行了调查。在研究区各农场采集有症状秋葵植物叶片样本(15份)。记录各亚样地的总株数和有症状株数,确定病害发生情况。采用双抗体夹心酶联免疫吸附试验(DAS- ELISA)检测秋葵花叶病毒,采用聚合酶链反应(PCR)检测秋葵卷叶病毒。结果表明,本研究中所有秋葵叶样品OLCV均为阴性,而OkMV均为阳性,卡杜纳州和扎姆法拉州的OkMV分别为20%和14%,而杂草样品中只有8个OKV呈阳性,但全部为OLCV阴性。
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引用次数: 0
期刊
Journal of Tropical Crop Science
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