Pub Date : 2019-12-01DOI: 10.19137/cienvet-201921201
M. Baez, O. Ortega, M. Lara, A. Arce
The present research work was carried out in 6 milking parlors of the Cordillera Department. The objective of the research was to determine the sensitivity to antimicrobials of Streptococcus spp. from milk samples from lactating cows positive to California Mastitis Test in the dairy farms mentioned in 2018. For this purpose, 200 bovine were counted, of milk aptitude, in lactation, without distinction of race or age, of which 141 (70,5%) were positive to the California Mastitis Test (CMT). The positive samples were identified, refrigerated and sent to the laboratory of the Department of Microbiology and Immunology of the Faculty of Veterinary Sciences of the National University of Asunción, where the corresponding microbiological analyzes were performed. The laboratory results obtained indicated that of the 141 samples analyzed in 89 (63,12%), bacterial microorganisms were isolated; 26 (29,21%) corresponding to Streptococcus spp.; of which 2 (7.69%) were sensitive to oxaciline; 19 (73,08%) to cefotaxime; 26 (100%) to penicillin; 22 (84,61%) to erythomycin; 25 (96,15%) to clindamycin; 24 (92,30%) to chloramphenicol; 15 (57,69%) to tetracycline; 22 (84,61%) at teicoplanine and 25 (96,15%) were sensitive to rifampicin.
{"title":"Determination of sensitivity of streptococcus spp antimicrobials in positive breastfeeding cows to california mastitis test in the cordillera department-paraguay","authors":"M. Baez, O. Ortega, M. Lara, A. Arce","doi":"10.19137/cienvet-201921201","DOIUrl":"https://doi.org/10.19137/cienvet-201921201","url":null,"abstract":"The present research work was carried out in 6 milking parlors of the Cordillera Department. The objective of the research was to determine the sensitivity to antimicrobials of Streptococcus spp. from milk samples from lactating cows positive to California Mastitis Test in the dairy farms mentioned in 2018. For this purpose, 200 bovine were counted, of milk aptitude, in lactation, without distinction of race or age, of which 141 (70,5%) were positive to the California Mastitis Test (CMT). The positive samples were identified, refrigerated and sent to the laboratory of the Department of Microbiology and Immunology of the Faculty of Veterinary Sciences of the National University of Asunción, where the corresponding microbiological analyzes were performed. The laboratory results obtained indicated that of the 141 samples analyzed in 89 (63,12%), bacterial microorganisms were isolated; 26 (29,21%) corresponding to Streptococcus spp.; of which 2 (7.69%) were sensitive to oxaciline; 19 (73,08%) to cefotaxime; 26 (100%) to penicillin; 22 (84,61%) to erythomycin; 25 (96,15%) to clindamycin; 24 (92,30%) to chloramphenicol; 15 (57,69%) to tetracycline; 22 (84,61%) at teicoplanine and 25 (96,15%) were sensitive to rifampicin.","PeriodicalId":33906,"journal":{"name":"Ciencia Veterinaria","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67728159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.19137/CIENVET-201921107
M. G. Cachau, Valeria Andrea Ferrero, M. Villagrán, Yesica Millahueque
Se plantearon propuestas de comunicacion y educacion para la salud desde un enfoque de trabajo intersectorial y de la promocion de la salud. Integraron el proyecto de extension universitaria docentes y estudiantes de la Facultad de Ciencias Veterinarias UNLPam y equipo del area programatica del Hospital Gobernador Centeno, General Pico, La Pampa. El objetivo fue promover una alimentacion saludable y practicas seguras de manipulacion de alimentos. Se participo en espacios intersectoriales convocados por Centros de Salud (CS) Brown y Rucci, se identificaron necesidades y planificaron intervenciones vinculadas con la alimentacion saludable. Las reuniones intersectoriales fueron convocadas por los CS y participaron referentes de distintas instituciones del area programatica de cada CS. Se realizaron talleres en diferentes ambitos: establecimientos educativos de nivel inicial y primario, centros de desarrollo infantil, merenderos, salas de espera, centros de formacion profesional, etc. Estas actividades permitieron identificar casos de vulnerabilidad, aclarar dudas y seguimiento por parte del equipo de salud. Se genero un espacio de dialogo y reflexion relacionados con alimentacion saludable y prevencion de enfermedades transmitidas por alimentos. Fue una posibilidad de articular las tareas de docencia, investigacion y extension. Fueron oportunidadesde ensenanza, aprendizaje y acercamiento a la practica profesional.
{"title":"Promoción de una alimentación saludable desde un enfoque de trabajo intersectorial y comunitario: experiencia de extensión universitaria en la Facultad de Ciencias Veterinarias","authors":"M. G. Cachau, Valeria Andrea Ferrero, M. Villagrán, Yesica Millahueque","doi":"10.19137/CIENVET-201921107","DOIUrl":"https://doi.org/10.19137/CIENVET-201921107","url":null,"abstract":"Se plantearon propuestas de comunicacion y educacion para la salud desde un enfoque de trabajo intersectorial y de la promocion de la salud. Integraron el proyecto de extension universitaria docentes y estudiantes de la Facultad de Ciencias Veterinarias UNLPam y equipo del area programatica del Hospital Gobernador Centeno, General Pico, La Pampa. El objetivo fue promover una alimentacion saludable y practicas seguras de manipulacion de alimentos. Se participo en espacios intersectoriales convocados por Centros de Salud (CS) Brown y Rucci, se identificaron necesidades y planificaron intervenciones vinculadas con la alimentacion saludable. Las reuniones intersectoriales fueron convocadas por los CS y participaron referentes de distintas instituciones del area programatica de cada CS. Se realizaron talleres en diferentes ambitos: establecimientos educativos de nivel inicial y primario, centros de desarrollo infantil, merenderos, salas de espera, centros de formacion profesional, etc. Estas actividades permitieron identificar casos de vulnerabilidad, aclarar dudas y seguimiento por parte del equipo de salud. Se genero un espacio de dialogo y reflexion relacionados con alimentacion saludable y prevencion de enfermedades transmitidas por alimentos. Fue una posibilidad de articular las tareas de docencia, investigacion y extension. Fueron oportunidadesde ensenanza, aprendizaje y acercamiento a la practica profesional.","PeriodicalId":33906,"journal":{"name":"Ciencia Veterinaria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48716830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.19137/CIENVET-201921101
Santiago Andrés Audisio, P. Vaquero, Edgardo Verna
El presente articulo hace revision de la proteina Osterix y el rol que cumple en la esqueletogenesis, la osificacion endocondral y en diversas patologias del sistema osteoarticular de los animales. Osterix es transcriptora de la familia de las proteinas morfogenicas osea que inducen la formacion de hueso. En la esqueletogenesis posibilita la diferenciacion de los preosteoblastos en osteoblastos y la maduracion y diferenciacion de osteoblastos en osteocitos. En la osificacion endocondral se expresa en los condrocitos maduros e hipermaduros y contribuye a degradar la matriz del cartilago. En la vida extrauterina colabora en la regulacion de la homeostasis osea e interviene en patologias osteoarticulares, oncologia osea y en la reparacion de las fracturas. Conocer la accion de Osterix sobre las celulas oseas y condrocitos es un punto de partida atractivo para futuras investigaciones con el fin de contribuir con el diagnostico y tratamiento de afecciones del sistema osteoarticular de los animales.
{"title":"The role of osterix in osteoblastic differentiation: Potential in the treatment of osteoarticular affections","authors":"Santiago Andrés Audisio, P. Vaquero, Edgardo Verna","doi":"10.19137/CIENVET-201921101","DOIUrl":"https://doi.org/10.19137/CIENVET-201921101","url":null,"abstract":"El presente articulo hace revision de la proteina Osterix y el rol que cumple en la esqueletogenesis, la osificacion endocondral y en diversas patologias del sistema osteoarticular de los animales. Osterix es transcriptora de la familia de las proteinas morfogenicas osea que inducen la formacion de hueso. En la esqueletogenesis posibilita la diferenciacion de los preosteoblastos en osteoblastos y la maduracion y diferenciacion de osteoblastos en osteocitos. En la osificacion endocondral se expresa en los condrocitos maduros e hipermaduros y contribuye a degradar la matriz del cartilago. En la vida extrauterina colabora en la regulacion de la homeostasis osea e interviene en patologias osteoarticulares, oncologia osea y en la reparacion de las fracturas. Conocer la accion de Osterix sobre las celulas oseas y condrocitos es un punto de partida atractivo para futuras investigaciones con el fin de contribuir con el diagnostico y tratamiento de afecciones del sistema osteoarticular de los animales.","PeriodicalId":33906,"journal":{"name":"Ciencia Veterinaria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49322257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.19137/CIENVET-201921105
Natalia Cazaux, A. R. Meder, C. Calvo, Guido Bertoldi, M. C. Miguel, Luciano Hartfiel
Dirofilaria Immitis is a zoonotic parasitosis produced by a nematode responsible for generating, in infected animals, potentially fatal cardiopulmonary disease. The dirofilariosis occurs in warm areas, with moisture, beeing the mosquito the vector responsible for its transmission. Despite its denomination, it is a mainly pulmonary disease, affecting the cardiac part in advanced stages. It has different larval stages, but stage L3 is infectious. It enters the host through the vector bite and begins to develop to adult stages which are located at the pulmonary arteries. The main definitive host and reservoir is the dog, but it can affect other wild canids like the fox. The clinical signs range from exercise intolerance, coughing, respiratory crackles and as the parasitic load progresses and increases, hemoptysis or epistaxis, weight loss, syncopes, may occur. In order to arrive at its diagnosis, the detection of microfilariae is essential, whether by taking blood smears from microhematocrit tubes or by the Knott test. Its treatment consists in, the elimination of the young filariae by the administration of macrocyclic lactones and then the use of an adulticide drug, melarsomine diclohydrate. The climatic changes that have occurred in the last time are of concern due to their propagation towards the north of Patagonia, and particularly to our province of La Pampa.
{"title":"Canine dirofilariasis, an emerging parasitism favoured by climate changes","authors":"Natalia Cazaux, A. R. Meder, C. Calvo, Guido Bertoldi, M. C. Miguel, Luciano Hartfiel","doi":"10.19137/CIENVET-201921105","DOIUrl":"https://doi.org/10.19137/CIENVET-201921105","url":null,"abstract":"Dirofilaria Immitis is a zoonotic parasitosis produced by a nematode responsible for generating, in infected animals, potentially fatal cardiopulmonary disease. The dirofilariosis occurs in warm areas, with moisture, beeing the mosquito the vector responsible for its transmission. Despite its denomination, it is a mainly pulmonary disease, affecting the cardiac part in advanced stages. It has different larval stages, but stage L3 is infectious. It enters the host through the vector bite and begins to develop to adult stages which are located at the pulmonary arteries. The main definitive host and reservoir is the dog, but it can affect other wild canids like the fox. The clinical signs range from exercise intolerance, coughing, respiratory crackles and as the parasitic load progresses and increases, hemoptysis or epistaxis, weight loss, syncopes, may occur. In order to arrive at its diagnosis, the detection of microfilariae is essential, whether by taking blood smears from microhematocrit tubes or by the Knott test. Its treatment consists in, the elimination of the young filariae by the administration of macrocyclic lactones and then the use of an adulticide drug, melarsomine diclohydrate. The climatic changes that have occurred in the last time are of concern due to their propagation towards the north of Patagonia, and particularly to our province of La Pampa.","PeriodicalId":33906,"journal":{"name":"Ciencia Veterinaria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48498310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.19137/cienvet-201921106
C. Tobal, R. H. Moralejo, Marcelo Rubén Sierro, D. Peratta, Juan Pablo Piccini, Facundo Lorenzo Hecker, Sebastián García
The beef cattle rearing is an economic and social activity in La Pampa province, developed in an area of natural forages, with severe weather and edaphic limitations. The present trial was carried out on a beef farm with 320 British crossbreed cattle, at Toay Department, La Pampa. During a period of five years, a protocol as following was implemented: three-time estrous synchronization followed by fixed-time artificial insemination plus natural mating until the end of the breeding period. The protocol used for artificial insemination started 40 days post-partum and each cow was classified by a colour ear tag and by probable date of delivery. At day zero, an intra-vaginal progesterone dispositive (0.5 g, i.v., Dib, Sintex S.A., Argentina) was placed to all cows in combination with an estradiol benzoate dose, when the dispositive was removed, after 7 days, the animals received a dose of prostaglandin (Cloprostenol, 2 cc., i.m., Ciclase DL, Sintex S.A.) and estradiol cypionate (1 mg, i.m. Cipiosyn, Sintex S.A., Argentina), to finally be inseminated at day 9. Thereafter, the protocol finished with the incoming of 5% of bulls until the end of the predetermined breeding period. The pregnancy percentage of both cows and heifers increased through the study period to values of 90 and 91%, respectively. 70% of parturition was concentrated during the first month, 23% in the second and just 3% during the last month of calving season. The suggested system allows increasing the reproductive efficiency of the cattle herd, achieving both a higher percentage of early pregnancy and increasing interparturition interval allowing a better recovery of the reproductive tract.
{"title":"Implementation of an integrated system of reproductive technology and management in a breeding cattle in the central semi-arid zone in the province of La Pampa","authors":"C. Tobal, R. H. Moralejo, Marcelo Rubén Sierro, D. Peratta, Juan Pablo Piccini, Facundo Lorenzo Hecker, Sebastián García","doi":"10.19137/cienvet-201921106","DOIUrl":"https://doi.org/10.19137/cienvet-201921106","url":null,"abstract":"The beef cattle rearing is an economic and social activity in La Pampa province, developed in an area of natural forages, with severe weather and edaphic limitations. The present trial was carried out on a beef farm with 320 British crossbreed cattle, at Toay Department, La Pampa. During a period of five years, a protocol as following was implemented: three-time estrous synchronization followed by fixed-time artificial insemination plus natural mating until the end of the breeding period. The protocol used for artificial insemination started 40 days post-partum and each cow was classified by a colour ear tag and by probable date of delivery. At day zero, an intra-vaginal progesterone dispositive (0.5 g, i.v., Dib, Sintex S.A., Argentina) was placed to all cows in combination with an estradiol benzoate dose, when the dispositive was removed, after 7 days, the animals received a dose of prostaglandin (Cloprostenol, 2 cc., i.m., Ciclase DL, Sintex S.A.) and estradiol cypionate (1 mg, i.m. Cipiosyn, Sintex S.A., Argentina), to finally be inseminated at day 9. Thereafter, the protocol finished with the incoming of 5% of bulls until the end of the predetermined breeding period. The pregnancy percentage of both cows and heifers increased through the study period to values of 90 and 91%, respectively. 70% of parturition was concentrated during the first month, 23% in the second and just 3% during the last month of calving season. The suggested system allows increasing the reproductive efficiency of the cattle herd, achieving both a higher percentage of early pregnancy and increasing interparturition interval allowing a better recovery of the reproductive tract.","PeriodicalId":33906,"journal":{"name":"Ciencia Veterinaria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43345354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.19137/cienvet-201921103
F. Toso, M. B. Lara, M. Mondino, R. Toso, Y. Marrón, A. Pombar
Previous studies have determined that hydro-alcoholic extracts of Equisetum giganteum (EE) and Cortaderia selloana (ECo) prevent gastric harm induced by stress in Mus musculus mice expose to hypothermia and immobilization. The aim of this study was to determine if the protective effect of indomethacin (ID), a non-steroid anti-inflammatory drug, on the gastric mucosa, is mediated by prostaglandin. The animals were assigned into one of the six groups of five animals each. The Blank Group (BG) received an oral dose (OD) of an excipient (Ex) composed of hydroxyl methyl cellulose and tweed 80; the Control Group (CG) received excipient plus 5 mg/kg subcutaneously of ID; the Equisetum Group (EG) with EE; the Equisetum Indomethacin Group (EIG) with EE and ID; the Cortaderia Group (CoG) with Eco and the Cortaderia Indomethacin Group (CoIG) with Co and ID. The doses DOI: http://dx.doi.org/10.19137/cienvet-201921103 45 utilized in the animals were obtained from 1 gram of dried aerial plant parts extracted with ethanol-water (1:1; v/v) and re-suspended with Ex until 0.5 ml. The ID groups (CG, EIG, CoIG) received the doses 1 hour before the beginning of the trial, and thereafter all the groups were subjected to stress during 4 hours at 22 °C. In order to assess the gastric harm, the percentage of ulcer inhibition was calculated as follow [(AUControl or Blank Groups – AUTreated Group/AUControl or Blank Group) x 100] where AU is the Ulcerated Area . The administration of ID induced a higher degree of gastric damage in the CG than in the BG (p≤0.01). The EE administration prevented the gastric damage in four mice of the EG, but the administration of ECo prevented the damage in all the animals of the CoG (p≤0.01), demonstrating no gastroprotective effect of EE when was given concomitantly with ID. The ECo showed significant gastroprotective effects when was administered simultaneously with ID (p≤0.01) suggesting that prostaglandin would have no effect in the protection of the gastric mucosa. The findings of vegetable drugs with the observed actions are of interest in order to both replace drugs with an antagonist of H2 and inhibitors of protons pump actions, because of its side effects when are prescribed during an extended period of time.
先前的研究已经确定，大木犀草(Equisetum giganteum, EE)和小木犀草(Cortaderia selloana, ECo)的水酒精提取物可以预防低温和固定环境下小家鼠应激引起的胃损伤。本研究的目的是确定非甾体抗炎药吲哚美辛(ID)对胃粘膜的保护作用是否由前列腺素介导。这些动物被分成六组，每组5只。空白组(BG)口服一种由羟基甲基纤维素和花呢80组成的赋形剂(Ex);对照组(CG)给予赋形剂加5mg /kg ID皮下注射;Equisetum Group (EG)与EE;消炎痛组(EIG)， EE和ID;含Eco的Cortaderia Group (CoG)和含Co和ID的Cortaderia indom美辛Group (CoIG)。动物使用的剂量DOI: http://dx.doi.org/10.19137/cienvet-201921103 45是从1克用乙醇-水(1:1;v/v)，再用Ex重新悬液至0.5 ml。ID组(CG、EIG、CoIG)在试验开始前1小时给药，然后在22℃下进行4小时应激。为了评估胃损伤，溃疡抑制的百分比计算如下[(AUControl或空白组- au治疗组/AUControl或空白组)x 100]，其中AU为溃疡区。ig组胃损伤程度高于BG组(p≤0.01)。EE对4只EG小鼠的胃有一定的保护作用，而ECo对CoG小鼠的胃有一定的保护作用(p≤0.01)，说明EE与ID同时给药时没有胃保护作用。ECo与ID同时给药时具有显著的胃保护作用(p≤0.01)，提示前列腺素对胃粘膜的保护作用不明显。植物药物的研究结果与观察到的作用是有趣的，因为它的副作用，当处方的时间较长时，既取代H2拮抗剂和质子泵作用抑制剂的药物。
{"title":"Gastroprotective effect of Equisetum giganteum L. n.v. cola de caballo and Cortaderia selloana (Schult & Schultz. f.) n.v. cortadera in mice submitted to stress and indomethacin","authors":"F. Toso, M. B. Lara, M. Mondino, R. Toso, Y. Marrón, A. Pombar","doi":"10.19137/cienvet-201921103","DOIUrl":"https://doi.org/10.19137/cienvet-201921103","url":null,"abstract":"Previous studies have determined that hydro-alcoholic extracts of Equisetum giganteum (EE) and Cortaderia selloana (ECo) prevent gastric harm induced by stress in Mus musculus mice expose to hypothermia and immobilization. The aim of this study was to determine if the protective effect of indomethacin (ID), a non-steroid anti-inflammatory drug, on the gastric mucosa, is mediated by prostaglandin. The animals were assigned into one of the six groups of five animals each. The Blank Group (BG) received an oral dose (OD) of an excipient (Ex) composed of hydroxyl methyl cellulose and tweed 80; the Control Group (CG) received excipient plus 5 mg/kg subcutaneously of ID; the Equisetum Group (EG) with EE; the Equisetum Indomethacin Group (EIG) with EE and ID; the Cortaderia Group (CoG) with Eco and the Cortaderia Indomethacin Group (CoIG) with Co and ID. The doses DOI: http://dx.doi.org/10.19137/cienvet-201921103 45 utilized in the animals were obtained from 1 gram of dried aerial plant parts extracted with ethanol-water (1:1; v/v) and re-suspended with Ex until 0.5 ml. The ID groups (CG, EIG, CoIG) received the doses 1 hour before the beginning of the trial, and thereafter all the groups were subjected to stress during 4 hours at 22 °C. In order to assess the gastric harm, the percentage of ulcer inhibition was calculated as follow [(AUControl or Blank Groups – AUTreated Group/AUControl or Blank Group) x 100] where AU is the Ulcerated Area . The administration of ID induced a higher degree of gastric damage in the CG than in the BG (p≤0.01). The EE administration prevented the gastric damage in four mice of the EG, but the administration of ECo prevented the damage in all the animals of the CoG (p≤0.01), demonstrating no gastroprotective effect of EE when was given concomitantly with ID. The ECo showed significant gastroprotective effects when was administered simultaneously with ID (p≤0.01) suggesting that prostaglandin would have no effect in the protection of the gastric mucosa. The findings of vegetable drugs with the observed actions are of interest in order to both replace drugs with an antagonist of H2 and inhibitors of protons pump actions, because of its side effects when are prescribed during an extended period of time.","PeriodicalId":33906,"journal":{"name":"Ciencia Veterinaria","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67728058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.19137/cienvet-201921104
Santiago Andrés Audisio, P. Vaquero, Edgardo Verna, A. Cristofolini, C. Merkis
Osteopontin (OPN) is the most abundant non-collagen protein in the bone matrix, where it fulfils the function of cellular adhesion and biomineralization. In the present work, the authors report the temporal and spatial localization of OPN during the repair of experimental orthopaedic bone defects treated with demineralized bone matrix (DBM) processed by the authors. 30 rabbits were used, which were given an orthopaedic bone defect of critical size in one of the radiuses, which was filled with DBM. The rabbits were euthanized in groups of 5 individuals at days 7, 15, 21, 30, 60 and 150. Histological cuts were immunomarked to establish the spatial and temporal immunomarcation of OPN. The histological cuts were observed with an optic microscope with which histological images were captured and analysed with the ImageJ software. The image analysis allowed the authors to establish the optic density (OD) and the integrated optic density (IOD). The data was analysed with the ANOVA and Fischer LSD tests. At day 7, the presence of OPN was observed only in the DBM particles, where the OD was 0.08 and the IOD was 1.64; at day 15, OPN marked different sites of collagen condensations and cells contained in the interior of the matrix. In this period the OD was 0.096 and the IOD, 9.26. At days 21 and 30, the OPN immunosignalled osteocytes, osteoblasts, osteoclasts and hypertrophic chondrocytes in the bone trabeculae adjacent
{"title":"Temporal and spatial immunolocalization of osteopontin in the repair of orthopaedic bone defects treated with demineralized bone matrix","authors":"Santiago Andrés Audisio, P. Vaquero, Edgardo Verna, A. Cristofolini, C. Merkis","doi":"10.19137/cienvet-201921104","DOIUrl":"https://doi.org/10.19137/cienvet-201921104","url":null,"abstract":"Osteopontin (OPN) is the most abundant non-collagen protein in the bone matrix, where it fulfils the function of cellular adhesion and biomineralization. In the present work, the authors report the temporal and spatial localization of OPN during the repair of experimental orthopaedic bone defects treated with demineralized bone matrix (DBM) processed by the authors. 30 rabbits were used, which were given an orthopaedic bone defect of critical size in one of the radiuses, which was filled with DBM. The rabbits were euthanized in groups of 5 individuals at days 7, 15, 21, 30, 60 and 150. Histological cuts were immunomarked to establish the spatial and temporal immunomarcation of OPN. The histological cuts were observed with an optic microscope with which histological images were captured and analysed with the ImageJ software. The image analysis allowed the authors to establish the optic density (OD) and the integrated optic density (IOD). The data was analysed with the ANOVA and Fischer LSD tests. At day 7, the presence of OPN was observed only in the DBM particles, where the OD was 0.08 and the IOD was 1.64; at day 15, OPN marked different sites of collagen condensations and cells contained in the interior of the matrix. In this period the OD was 0.096 and the IOD, 9.26. At days 21 and 30, the OPN immunosignalled osteocytes, osteoblasts, osteoclasts and hypertrophic chondrocytes in the bone trabeculae adjacent","PeriodicalId":33906,"journal":{"name":"Ciencia Veterinaria","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67728088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.19137/cienvet-201820204
Alejandra Edith Antruejo, Juan Pablo Savoy, Cristian Hernán Perrotta, Z. Canet, A. M. Dottavio, R. J. Masso
El pollo Campero representa una alternativa productiva en el marco de sistemas avicolas semi-intensivos que buscan preservar el bienestar animal. El protocolo de produccion establece restricciones vinculadas con estrategias de manejo entre las que se encuentra la densidad maxima de aves permitida por unidad de superficie. El objetivo de este trabajo fue evaluar el efecto de la modificacion de la densidad de alojamiento sobre el patron dinamico del crecimiento, la uniformidad por precision del peso corporal, la conformacion corporal y los caracteres a la faena en pollos machos del cruzamiento experimental de tres vias Campero Casilda. Las aves se criaron como un unico grupo, en confinamiento, hasta los 35 dias, edad a partir de la cual se distribuyeron aleatoriamente en tres tratamientos: Densidad Baja: 6 aves/m2 (21 kg/m2), Densidad Recomendada: 7 aves/m2 (24,5 kg/m2) y Densidad Alta: 8 aves/m2 (28 kg/m2) y se habilito su acceso a parque hasta su faena a los 84 dias. Dentro de los limites ensayados en este trabajo, ni la disminucion de la densidad indicada por el protocolo de produccion de pollos camperos, en busca de mayor bienestar, ni su aumento, en busca de mayor rentabilidad, afectaron en forma significativa el crecimiento evaluado a partir del patron dinamico del peso corporal y del registro de indicadores de conformacion basados en medidas lineales, Tampoco se vieron afectados la uniformidad en peso corporal, un indicador de trascendencia en la produccion avicola, ni la proporcion de cortes valiosos de interes carnicero y grasa abdominal ni el rendimiento a la faena. Los resultados indican que el aumento de la densidad no produce efectos detrimentales sobre los caracteres de mayor trascendencia economica, en particular, el peso corporal y los caracteres a la faena, por lo que podria introducirse una modificacion en este sentido en el protocolo tendiente a favorecer el retorno economico en emprendimientos productivos
在寻求保护动物福利的半集约化家禽系统框架内，野鸡是一种多产的替代方案。生产协议规定了与管理策略相关的限制，包括单位面积允许的最大鸟类密度。本研究的目的是评价饲养密度的变化对三通试验杂交Campero Casilda公鸡动态生长模式、体重精度均匀性、体形和屠宰性状的影响。成长成一个单一的群体,鸟类监禁,直到35天,是从这随机发放了3个疗程:低密度6只/ m2 (21 kg / m2),密度建议:7 / m2 (24,5 kg / m2)和鸟类密度高鸟:8 / 28平方米(kg / m2),并获得重建公园到84天的工作。极限他们靠什么在这工作,没有密度disminucion匹配的议定书的鸡camperos,寻找更多的福利,甚至增加,寻找最高的回报,评估影响显著增长模式起dinamico记录体重和线性conformacion基于指标,也没有受到干扰,标准化的体重,这是家禽生产中重要的指标，既不是有价值的肉块和腹部脂肪的比例，也不是农场的产量。研究结果表明,增加密度,它不产生影响detrimentales economica的一项最重要的文字字符,特别是体重和责任,因此可能会进入一种modificacion议定书在这方面鼓励返回南极容易在生产企业
{"title":"Densidad de alojamiento y caracteres productivos en un cruzamiento experimental de tres vías de pollo campero","authors":"Alejandra Edith Antruejo, Juan Pablo Savoy, Cristian Hernán Perrotta, Z. Canet, A. M. Dottavio, R. J. Masso","doi":"10.19137/cienvet-201820204","DOIUrl":"https://doi.org/10.19137/cienvet-201820204","url":null,"abstract":"El pollo Campero representa una alternativa productiva en el marco de sistemas avicolas semi-intensivos que buscan preservar el bienestar animal. El protocolo de produccion establece restricciones vinculadas con estrategias de manejo entre las que se encuentra la densidad maxima de aves permitida por unidad de superficie. El objetivo de este trabajo fue evaluar el efecto de la modificacion de la densidad de alojamiento sobre el patron dinamico del crecimiento, la uniformidad por precision del peso corporal, la conformacion corporal y los caracteres a la faena en pollos machos del cruzamiento experimental de tres vias Campero Casilda. Las aves se criaron como un unico grupo, en confinamiento, hasta los 35 dias, edad a partir de la cual se distribuyeron aleatoriamente en tres tratamientos: Densidad Baja: 6 aves/m2 (21 kg/m2), Densidad Recomendada: 7 aves/m2 (24,5 kg/m2) y Densidad Alta: 8 aves/m2 (28 kg/m2) y se habilito su acceso a parque hasta su faena a los 84 dias. Dentro de los limites ensayados en este trabajo, ni la disminucion de la densidad indicada por el protocolo de produccion de pollos camperos, en busca de mayor bienestar, ni su aumento, en busca de mayor rentabilidad, afectaron en forma significativa el crecimiento evaluado a partir del patron dinamico del peso corporal y del registro de indicadores de conformacion basados en medidas lineales, Tampoco se vieron afectados la uniformidad en peso corporal, un indicador de trascendencia en la produccion avicola, ni la proporcion de cortes valiosos de interes carnicero y grasa abdominal ni el rendimiento a la faena. Los resultados indican que el aumento de la densidad no produce efectos detrimentales sobre los caracteres de mayor trascendencia economica, en particular, el peso corporal y los caracteres a la faena, por lo que podria introducirse una modificacion en este sentido en el protocolo tendiente a favorecer el retorno economico en emprendimientos productivos","PeriodicalId":33906,"journal":{"name":"Ciencia Veterinaria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44911240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.19137/cienvet-201820202
P. Beltrán, de L. R. Corrêa, A. Rohden, R. Aiolifi, A. B. Soares
The use of management strategies, such as adjustment of grazing intensity and nitrogen fertilization, are fundamental for the success of animal production, in crop-livestock integration systems (IAG). The objective was to evaluate the effects of grazing intensity and nitrogen fertilization on the nutritional value of Italian ragweed pastures (Lolium multiflorum Lam.), individual performance and by area of steers Nelore x Charolais IAG. A randomized block experimental design with 2 x 2 factorial application and three repetitions was used. The first factor was grass height: High Height 25 cm (AA) and Low Height 10 cm (BA). The second factor was the period of nitrogen fertilization applied at two different times in the system: N applied in the pasture (NP) and N applied in the grains crop (NG), in doses of 200 kg of N ha-1, forming 4 treatments: AANG, AANP, BANG and BANP. The grass height of Italian raigras (Lolium multiflorum Lam.) was regulated by continuous grazing with variable number of animals. With nitrogen fertilization in the pasture, it is possible to handle the Raigras ‘Winter star’ grasses with an average height of 12.5 cm. The accumulation rate and the total DM production were higher in AANP. Nitrogen fertilization of the pasture resulted in higher crude protein content, less of neutral detergent fiber and acid detergent fiber. With the advance of the grazing period and the pasture phenology, there was a decrease in crude protein and an increase in the neutral and acid detergent fiber. The average daily gain did not present a difference between treatments and was greater than 1.0 kg day-1, the average gain per area was greater in the BANP treatment
在作物-牲畜一体化系统（IAG）中，使用管理策略，如调整放牧强度和氮肥，是动物生产成功的基础。目的是评估放牧强度和施氮对意大利豚草牧场（Lolium multiflorum Lam.）营养价值、个体性能和面积的影响。采用2×2析因应用和三次重复的随机分组实验设计。第一个因素是草高：高25厘米（AA）和低10厘米（BA）。第二个因素是系统中两个不同时期的施氮时间：牧场施氮（NP）和粮食作物施氮（NG），剂量为200 kg N ha-1，形成4个处理：AANG、AANP、BANG和BANP。意大利赖草（Lolium multiflorum Lam.）的草高是通过连续放牧和不同数量的动物来调节的。通过牧场施氮，可以处理平均高度为12.5cm的Raigras‘Winter star’草。AANP的积累率和总DM产量较高。牧场施用氮肥后，粗蛋白含量较高，中性洗涤纤维和酸性洗涤纤维含量较低。随着放牧期和牧草酚期的延长，粗蛋白含量下降，中性和酸性洗涤纤维含量增加。处理之间的平均日增重没有差异，并且大于1.0 kg第1天，BANP处理中的每个区域的平均增重更大
{"title":"Valor Nutritivo de pasturas de Lolium multiflorum y desempeño de novillos Charolais X Nelore en sistema de integración Agricultura-Ganadería","authors":"P. Beltrán, de L. R. Corrêa, A. Rohden, R. Aiolifi, A. B. Soares","doi":"10.19137/cienvet-201820202","DOIUrl":"https://doi.org/10.19137/cienvet-201820202","url":null,"abstract":"The use of management strategies, such as adjustment of grazing intensity and nitrogen fertilization, are fundamental for the success of animal production, in crop-livestock integration systems (IAG). The objective was to evaluate the effects of grazing intensity and nitrogen fertilization on the nutritional value of Italian ragweed pastures (Lolium multiflorum Lam.), individual performance and by area of steers Nelore x Charolais IAG. A randomized block experimental design with 2 x 2 factorial application and three repetitions was used. The first factor was grass height: High Height 25 cm (AA) and Low Height 10 cm (BA). The second factor was the period of nitrogen fertilization applied at two different times in the system: N applied in the pasture (NP) and N applied in the grains crop (NG), in doses of 200 kg of N ha-1, forming 4 treatments: AANG, AANP, BANG and BANP. The grass height of Italian raigras (Lolium multiflorum Lam.) was regulated by continuous grazing with variable number of animals. With nitrogen fertilization in the pasture, it is possible to handle the Raigras ‘Winter star’ grasses with an average height of 12.5 cm. The accumulation rate and the total DM production were higher in AANP. Nitrogen fertilization of the pasture resulted in higher crude protein content, less of neutral detergent fiber and acid detergent fiber. With the advance of the grazing period and the pasture phenology, there was a decrease in crude protein and an increase in the neutral and acid detergent fiber. The average daily gain did not present a difference between treatments and was greater than 1.0 kg day-1, the average gain per area was greater in the BANP treatment","PeriodicalId":33906,"journal":{"name":"Ciencia Veterinaria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42950413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.19137/cienvet-201820203
J. Macario, Malargüe Argentina Inta Aer Malargüe, P. Dayenoff, Pablo Dri, El Vivero s Inta Rama Caída, Dirección Provincial de Ganadería de Mendoza. Argentina
The objective of the present study was to evaluate some socio-productive aspects of goat producers in the area of Malargue, Mendoza, including within the strip of owners of between 480-640 animal, sector which accounts for 294 establishments, 23.4% of the total of goat farms in the region. A structured survey was organized for this, where age, formal education, knowledge of technology, importance of marketing, reproduction, animal health, associations, among others were consulted. For the statistical study of the data applied analysis of variance with Tukey tests for media differentiation was used, for the quantitative information and for the study of the qualitative variables a contingency table 2 X, with p≤0 , 05 for the characterization of groups was used. Results showed that 83.3% of producers are over 45 years of age, only 38% has complete primary education, 54.2% admitted knowing the existence of technology to improve their productive system, 77% does not give importance to reproductive management of their herd, only 10.4% believed in the importance of health, while 79% said that the associations would improve their income. In conclusion only one third of the producers has completed the primary cycle of formal education, most of goat producers in this sector have access to and knowledge of technologies, in spite of this, they have low consideration towards the need for applying reproductive management and health rules and a very important percentage considered associations to improve economic income.
{"title":"Valoración de algunos aspectos socioproductivos, del productor caprino de Malargüe, Mendoza","authors":"J. Macario, Malargüe Argentina Inta Aer Malargüe, P. Dayenoff, Pablo Dri, El Vivero s Inta Rama Caída, Dirección Provincial de Ganadería de Mendoza. Argentina","doi":"10.19137/cienvet-201820203","DOIUrl":"https://doi.org/10.19137/cienvet-201820203","url":null,"abstract":"The objective of the present study was to evaluate some socio-productive aspects of goat producers in the area of Malargue, Mendoza, including within the strip of owners of between 480-640 animal, sector which accounts for 294 establishments, 23.4% of the total of goat farms in the region. A structured survey was organized for this, where age, formal education, knowledge of technology, importance of marketing, reproduction, animal health, associations, among others were consulted. For the statistical study of the data applied analysis of variance with Tukey tests for media differentiation was used, for the quantitative information and for the study of the qualitative variables a contingency table 2 X, with p≤0 , 05 for the characterization of groups was used. Results showed that 83.3% of producers are over 45 years of age, only 38% has complete primary education, 54.2% admitted knowing the existence of technology to improve their productive system, 77% does not give importance to reproductive management of their herd, only 10.4% believed in the importance of health, while 79% said that the associations would improve their income. In conclusion only one third of the producers has completed the primary cycle of formal education, most of goat producers in this sector have access to and knowledge of technologies, in spite of this, they have low consideration towards the need for applying reproductive management and health rules and a very important percentage considered associations to improve economic income.","PeriodicalId":33906,"journal":{"name":"Ciencia Veterinaria","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43132795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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