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A Review on the Resistance and Accumulation of Heavy Metals by Different Microbial Strains 不同微生物菌株对重金属的抗性及积累研究进展
Pub Date : 2022-01-07 DOI: 10.5772/intechopen.101613
Madhuri Girdhar, Zeba Tabassum, K. Singh, Aarthi R. Mohan
Heavy metals accumulated the earth crust and causes extreme pollution. Accumulation of rich concentrations of heavy metals in environments can cause various human diseases which risks health and high ecological issues. Mercury, arsenic, lead, silver, cadmium, chromium, etc. are some heavy metals harmful to organisms at even very low concentration. Heavy metal pollution is increasing day by day due to industrialization, urbanization, mining, volcanic eruptions, weathering of rocks, etc. Different microbial strains have developed very efficient and unique mechanisms for tolerating heavy metals in polluted sites with eco-friendly techniques. Heavy metals are group of metals with density more than 5 g/cm3. Microorganisms are generally present in contaminated sites of heavy metals and they develop new strategies which are metabolism dependent or independent to tackle with the adverse effects of heavy metals. Bacteria, Algae, Fungi, Cyanobacteria uses in bioremediation technique and acts a biosorbent. Removal of heavy metal from contaminated sites using microbial strains is cheaper alternative. Mostly species involved in bioremediation include Enterobacter and Pseudomonas species and some of bacillus species too in bacteria. Aspergillus and Penicillin species used in heavy metal resistance in fungi. Various species of the brown algae and Cyanobacteria shows resistance in algae.
重金属在地壳中积聚,造成严重污染。环境中高浓度重金属的积累可引起各种危害健康的人类疾病和严重的生态问题。汞、砷、铅、银、镉、铬等是一些重金属,即使浓度很低也对生物体有害。由于工业化、城市化、采矿、火山喷发、岩石风化等原因,重金属污染日益严重。不同的微生物菌株已经发展出非常有效和独特的机制,以生态友好的技术在污染场地耐受重金属。重金属是指密度大于5克/立方厘米的一组金属。微生物普遍存在于重金属污染场所,它们发展出依赖或独立代谢的新策略来应对重金属的不利影响。细菌,藻类,真菌,蓝藻用于生物修复技术,并作为生物吸附剂。利用微生物菌株从污染场地去除重金属是一种更便宜的选择。参与生物修复的主要物种包括肠杆菌和假单胞菌,以及细菌中的一些芽孢杆菌。曲霉和盘尼西林种用于真菌对重金属的抗性。各种褐藻和蓝藻在藻类中表现出抗性。
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引用次数: 4
Effects of Biochar in Soil and Water Remediation: A Review 生物炭在土壤和水修复中的作用研究进展
Pub Date : 2021-12-15 DOI: 10.5772/intechopen.101374
A. Perdigão, José Luís da Silva Pereira
In the last decades increased global environmental concerns to water and soils pollution. The main concerns are related to the contamination of the ecosystem, food security, and human health since many of the contaminants present in soil and water (residues of pesticides and antibiotics, genes of resistance to antibiotics, and heavy metals) are absorbed by plants and enter the food chain. Remediation of the contaminated water and soil to ensure sustainable water supply and food production is urgently needed. The use of biochar can have a positive effect on this remediation process. There are several studies that demonstrate the biochar’s ability to block/reduce the contaminating effect of pesticides, antibiotic residues, antibiotic resistance genes, and heavy metals. The objective of this chapter is to carry out a comprehensive review of the effect of using biochar on the availability/transmission of these contaminants to the soil and food supply chain.
在过去的几十年里,全球对水和土壤污染的关注日益增加。主要问题与生态系统、粮食安全和人类健康的污染有关,因为土壤和水中存在的许多污染物(农药和抗生素残留、抗生素抗性基因和重金属)被植物吸收并进入食物链。迫切需要修复受污染的水和土壤,以确保可持续的供水和粮食生产。生物炭的使用可以对这一修复过程产生积极的影响。有几项研究表明,生物炭能够阻止/减少农药、抗生素残留、抗生素抗性基因和重金属的污染效应。本章的目的是对使用生物炭对这些污染物进入土壤和食品供应链的可用性/传输的影响进行全面审查。
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引用次数: 3
Mass Spectrometry Coupled with Chromatography toward Separation and Identification of Organic Mixtures 质谱联用色谱在有机混合物分离鉴定中的应用
Pub Date : 2021-11-22 DOI: 10.5772/intechopen.100517
A. Bouziani, Mohamed Yahya
Mass spectrometers can provide information about molecular composition and chemical structure. However, with complex mixtures, superpositions and even suppression of signals may occur. On the other hand, Chromatography is an ideal technique for separating complexes but is often insufficient for compound identification. Hence, coupling both techniques in order to eliminate the limitations of each technique makes perfect sense. In this contribution, a brief description of mass spectrometry coupled with chromatography in the gas and liquid phase will be discussed to explain the advantages of coupling the two methods. The ionization techniques are also reported and followed by application areas of these techniques. Finally, the recording and treatment of the results are reviewed.
质谱仪可以提供有关分子组成和化学结构的信息。然而,在复杂的混合物中,可能会出现信号的叠加甚至抑制。另一方面,色谱法是分离配合物的理想技术,但往往不足以用于化合物鉴定。因此,将这两种技术结合起来以消除每种技术的局限性是非常有意义的。在这篇文章中,将简要介绍气相和液相中质谱法与色谱法的耦合,以解释耦合这两种方法的优点。对离子化技术进行了报道,并介绍了离子化技术的应用领域。最后,对实验结果的记录和处理进行了综述。
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引用次数: 0
Differential Impact of the Prior Mix by Stirring in the Biodegradation of Sunflower Oil 混合搅拌对葵花籽油生物降解的差异性影响
Pub Date : 2021-11-11 DOI: 10.5772/intechopen.100480
Pedro Eulogio Cisterna Osorio, Barbara Faundez-Miño
Fats and oils present in wastewater are usually eliminated by physical and biological processes. In this experience, the fatty wastewaters are treated biologically, and it assesses the impact of the mix in the fats and oils biodegradation and carried out the experiments in a laboratory scale unit. The biodegradation of fats and oils was analysed in two sceneries, with mix previous by mechanical agitation and without mix. Key parameters were monitored, such as the concentration of fats and oils in the influents and effluents, mass loading, and the efficiency of biodegradation. The mass loading range was similar in both sceneries. In the experimental activated sludge plant without mix, the biodegradation of fats and oils reached levels in the range of 28 to 42.5%. For the wastewater treatment plant with a previous mix by mechanical agitation, the levels of biodegradation of fats and oils ranged from 64 to 75%. Therefore, considering the efficiency of the biodegradation of fats and oils in both sceneries, the results indicated that the level mix is a high incidence.
废水中存在的油脂通常通过物理和生物过程消除。在此经验中,对含脂肪废水进行了生物处理,并评估了油脂混合物对生物降解的影响,并在实验室规模装置中进行了实验。对油脂的生物降解进行了两种情况下的分析,即机械搅拌混合和不搅拌混合。监测了关键参数,如进水和出水中脂肪和油的浓度、质量负荷和生物降解效率。在两种情况下,质量加载范围是相似的。在无混合的活性污泥试验装置中,油脂的生物降解率达到28% ~ 42.5%。对于先前通过机械搅拌混合的废水处理厂,脂肪和油的生物降解水平从64到75%不等。因此,考虑到两种情况下油脂的生物降解效率,结果表明混合水平是高发生率的。
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引用次数: 0
Bioremediation Techniques for Soil Pollution: An Introduction 土壤污染生物修复技术综述
Pub Date : 2021-09-14 DOI: 10.5772/intechopen.99028
A. Verma
Environmental pollution has been on the rise in the past few decades owing to increased human activities on energy reservoirs, unsafe agricultural practices and rapid industrialization. Soil pollution is one of the major worry among all because soil contamination can harm the humans by consumption of food grown in polluted soil or it can cause infertility to soil and lower the productivity, Among the pollutants that are of environmental and public health concerns due to their toxicities are: heavy metals, nuclear wastes, pesticides, greenhouse gases, and hydrocarbons. So this chapter will include; Sources of soil pollution and remediation of polluted sites using biological means has proven effective and reliable due to its eco-friendly features. Bio-remediation can either be carried out ex situ or in situ, depending on several factors, which include site characteristics, type and concentration of pollutants. It also seen as a solution for emerging contaminant problems.
过去几十年来,由于人类对水库活动的增加、不安全的农业做法和迅速的工业化,环境污染一直在增加。土壤污染是所有人的主要担忧之一,因为土壤污染可以通过食用在污染土壤中生长的食物来伤害人类,或者它可以导致土壤不孕症和降低生产力,由于其毒性而引起环境和公共卫生问题的污染物包括:重金属,核废料,农药,温室气体和碳氢化合物。这一章将包括;土壤污染源和污染场地的生物修复因其生态友好的特点而被证明是有效和可靠的。生物修复既可以在原地进行,也可以在原地进行,这取决于几个因素,包括场地特征、污染物的类型和浓度。它也被视为解决新出现的污染问题的办法。
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引用次数: 5
Phytochelatin Synthase in Heavy Metal Detoxification and Xenobiotic Metabolism 植物螯合素合成酶在重金属解毒和外源代谢中的作用
Pub Date : 2021-09-14 DOI: 10.5772/intechopen.99077
Ju-Chen Chia
Phytochelatin synthase (PCS) is well-known for its role in heavy metal detoxification in plants, yeasts and non-vertebrate animals. It is a protease-like enzyme that catalyzes glutathione (GSH) to form phytochelatins (PCs), a group of Cys-rich and non-translational polypeptides with a high affinity to heavy metals. In addition, PCS also functions in xenobiotic metabolism by processing GS-conjugates in the cytosol. Because PCS is involved in GSH metabolism and the degradation of GS-conjugates, it is one of the important components in GSH homeostasis and GSH-mediated biodegradation. This chapter reviews the biochemical mechanism of PCS, how the enzyme activity is regulated, and its roles in heavy metal detoxification as well as GS-S-conjugate metabolism. This chapter also highlights the potential applications of PCS in the improvement of plant performance under combined stresses.
植物螯合素合成酶(Phytochelatin synthase, PCS)因其在植物、酵母和非脊椎动物体内的重金属解毒作用而闻名。它是一种蛋白酶样酶,催化谷胱甘肽(GSH)形成植物螯合素(PCs),这是一组富含cys的非翻译多肽,对重金属具有高亲和力。此外,PCS还通过处理细胞质中的gs偶联物来参与外源代谢。由于PCS参与GSH代谢和gs -缀合物的降解,因此它是GSH稳态和GSH介导的生物降解的重要成分之一。本章综述了PCS的生化机制、活性调控机制及其在重金属解毒和gs - s缀合物代谢中的作用。本章还重点介绍了PCS在复合胁迫下改善植物性能方面的潜在应用。
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引用次数: 3
Persistant Organic Pollutants in Soil and Its Phytoremediation 土壤中持久性有机污染物及其植物修复
Pub Date : 2021-09-13 DOI: 10.5772/intechopen.99835
M. Begum, B. Sarmah, G. G. Kandali, S. Kalita, Ipsita Ojha, Raktim Bhagawati, Lipika Talukdar
Persistent organic pollutants (POPs) of soil mainly exhibit toxic characteristics that posses hazard to whole mankind. These toxic pollutants includes several group of compound viz., polychlorinated biphenyls, polybrominated biphenyls, polychlorinated dibenzofurans, polycyclic aromatic hydrocarbons, organophosphorus and carbamate insecticides, herbicides and organic fuels, especially gasoline and diesel. They can also be complex mixture of organic chemicals, heavy metals and microbes from septic systems, animal wastes and other sources of organic inputs. Phytoremediation is an emerging technology which can be used for remediation of soil from organic pollutants. In this chapter an attempt has been made to discuss about the sources of organic pollutants, factors that influenced the uptake of organic pollutants by plants, the different mechanism responsible for organic pollutants, phytoremediation of organic pollutants and their advantages and limitation.
土壤中持久性有机污染物主要表现为毒性特征,对全人类具有危害性。这些有毒污染物包括几类化合物,即多氯联苯、多溴联苯、多氯二苯并呋喃、多环芳烃、有机磷和氨基甲酸酯杀虫剂、除草剂和有机燃料,特别是汽油和柴油。它们也可能是来自化粪池系统、动物粪便和其他有机投入来源的有机化学品、重金属和微生物的复杂混合物。植物修复是一种新兴的修复土壤有机污染物的技术。本章对有机污染物的来源、影响植物吸收有机污染物的因素、有机污染物的不同机制、有机污染物的植物修复及其优点和局限性进行了探讨。
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引用次数: 3
Aquatic Plants as Bioremediators in Pollution Abatement of Heavy Metals 水生植物在重金属污染治理中的生物修复作用
Pub Date : 2021-08-27 DOI: 10.5772/intechopen.99627
A. V. Meera, Manorama Thampatti Kc, J. John, B. Sudha, A. Sajeena
Over use of chemical inputs and exploitation of natural resources have degraded our ecosystem to a large extent. Our water bodies are drastically affected, especially due to the impact of heavy metal loading. The biomagnification that results from these difficult to degrade metals is naturally affecting the human health. The physical and chemical methods commonly employed for water purification are not only highly expensive but also further aggravate the pollution problem. Hence, all efforts must be taken to exploit the emerging green technology approach in pollution remediation. Several aquatic plants have specific affinity towards heavy metals and they flourish well in this contaminated environment. The common mechanisms of phytoremediation and varied type of aquatic plants with high remediation potential are reviewed in this chapter.
过度使用化学投入品和开发自然资源在很大程度上使我们的生态系统退化。我们的水体受到了极大的影响,特别是由于重金属负荷的影响。这些难以降解的金属产生的生物放大效应自然会影响人体健康。通常用于水净化的物理和化学方法不仅价格昂贵,而且还进一步加剧了污染问题。因此,必须尽一切努力在污染补救中利用新兴的绿色技术方法。一些水生植物对重金属具有特殊的亲和力,它们在这种污染的环境中生长良好。本章综述了植物修复的常见机制和具有较高修复潜力的水生植物类型。
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引用次数: 0
Phytoremediation of Arsenic Contaminated Water Using Aquatic, Semi-Aquatic and Submerged Weeds 水生、半水生和沉水杂草对砷污染水体的植物修复
Pub Date : 2021-08-07 DOI: 10.5772/intechopen.98961
D. Roy, D. Sreekanth, Deepak P. Pawar, H. Mahawar, K. K. Barman
Arsenic (As) is the one the most toxic element present in earth which poses a serious threat to the environment and human health. Arsenic contamination of drinking water in South and Southeast Asia reported one of the most threatening problems that causes serious health hazard of millions of people of India and Bangladesh. Further, use of arsenic contaminated ground water for irrigation purpose causes entry of arsenic in food crops, especially in Rice and other vegetable crops. Currently various chemical technologies utilized for As removal from contaminated water like adsorption and co-precipitation using salts, activated charcoal, ion exchange, membrane filtration etc. are very costly and cannot be used for large scale for drinking and agriculture use. In contrast, phytoremediation utilizes green plats to remove pollutants from contaminated water using various mechanisms such as rhizofiltration, phytoextraction, phytostabilization, phytodegrartion and phytovolatilization. A large numbers of terrestrial and aquatic weed flora have been identified so far having hyper metal, metalloid and organic pollutant removal capacity. Among the terrestrial weed flora Arundo donax, Typha latifolia, Typha angustifolia, Vetivaria zizinoids etc. are the hyper As accumulator. Similarly Eicchornea crassipes (Water hyacinth), Pistia stratiotes (water lettuce), Lemna minor (duck weed), Hyrdilla verticillata, Ceratophyllum demersum, Spirodella polyrhiza, Azola, Wolfia spp., etc. are also capable to extract higher amount of arsenic from contaminated water. These weed flora having As tolerance mechanism in their system and thus remediate As contaminated water vis-à-vis continue their life cycle. In this chapter we will discuss about As extraction potential of various aquatic and semi aquatic weeds from contaminated water, their tolerance mechanism, future scope and their application in future world mitigating As contamination in water resources.
砷是地球上存在的毒性最大的元素之一,对环境和人类健康构成严重威胁。据报告,南亚和东南亚的饮用水砷污染是对印度和孟加拉国数百万人造成严重健康危害的最具威胁性的问题之一。此外,使用受砷污染的地下水进行灌溉导致砷进入粮食作物,特别是水稻和其他蔬菜作物。目前用于去除污染水中砷的各种化学技术,如盐吸附共沉淀法、活性炭法、离子交换法、膜过滤法等,成本很高,不能大规模用于饮用水和农业。相比之下,植物修复利用绿色植物通过各种机制,如根过滤、植物提取、植物稳定、植物降解和植物挥发,从污染水中去除污染物。目前已发现大量陆生和水生杂草植物群具有去除超金属、类金属和有机污染物的能力。在陆生杂草植物群中,黄花蒿、黄花蒿、黄花蒿、黄花蒿等都是高砷积累者。同样,Eicchornea crassipes(水葫芦)、Pistia stratiotes(水莴苣)、lena minor(鸭草)、hydrrilla verticillata、Ceratophyllum demersum、Spirodella polyrhiza、Azola、Wolfia spp等也能够从受污染的水中提取更多的砷。这些杂草植物在其系统中具有耐砷机制,从而对砷污染的水进行修复,从而继续其生命周期。本章将讨论各种水生和半水生杂草从污染水体中提取砷的潜力、耐砷机理、未来的应用范围及其在未来世界减轻水资源砷污染中的应用。
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引用次数: 2
Bacillus megaterium Biodegradation Glyphosate 巨芽孢杆菌生物降解草甘膦
Pub Date : 2021-08-03 DOI: 10.5772/intechopen.96919
Nibal Khaleel Mousa, Abdul-Jabbar A. Ali, M. Hussein
The Bacillus megaterium ability was evaluated in this paper to degrade the Glyphosate. organophosphorus pesticides, The bacteria re-cultured that isolated from other researches of Baghdad soils and morphological identification and biochemical tests besides by selectivity media. The (5 and 25) ppm showed the highest growth results were within two days to two months on mineral salt media. The highest glyphosate degradation ratio % were (70) % per 25 ppm/two months. Incubation period Increasing led to highest glyphosate degradation ratio% at (25) ppm led to conclusion that bacteria digestive the pesticides as carbon and nitrogen sources and will be well harvest it form contaminated areas.
研究了巨芽孢杆菌对草甘膦的降解能力。有机磷农药、从巴格达土壤中分离的细菌再培养、形态鉴定和生化试验以及选择性培养基。在(5和25)ppm条件下,无机盐培养基在2天至2个月内生长效果最好。最高的草甘膦降解率%为每25 ppm/ 2个月(70)%。孵育期的增加导致(25)ppm时草甘膦降解率最高,从而得出结论,细菌将农药作为碳和氮源消化,并将其从污染区域中很好地收获。
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引用次数: 3
期刊
Biodegradation [Working Title]
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