The objective of this study was to determine some physiological properties (mass ratio, moisture) and biochemical properties (the content of the chlorophyll, carotenoids and the total polyphenol (TPC)) of Musa paradisiaca and Musa acuminata AA banana varieties peel, which were harvested in Tien Giang province, Vietnam at 03 stages of ripeness (I, III and VI in the color index 08 ripeness of banana). The results showed that, the ripening from I to VI maturity had a significant effected on the mass ratio, the moisture, the chlorophyll, the carotenoids and the TPC in 02 banana varieties peel and following different trends. The mass ratio and the moisture decreased, the chlorophyll and the carotenoids content recorded as opposite trends with degradation of chlorophyll and synthesis of carotenoids occured simultaneously, the most specially, the TPC increased and reached to maximum value at III maturity and decreased after. Results suggest that two banana varieties peel could be effectively used for extraction of polyphenol compounds in the future.
{"title":"Some changes in physiological and biochemical properties of Musa paradisiaca and Musa acuminata AA banana peel during ripening harvested in Tien Giang Province, Viet Nam","authors":"Truong Quoc Tat, Nguyen Thi Ngoc Tham, Nguyen Duy Khanh","doi":"10.46223/hcmcoujs.tech.en.12.1.2034.2022","DOIUrl":"https://doi.org/10.46223/hcmcoujs.tech.en.12.1.2034.2022","url":null,"abstract":"The objective of this study was to determine some physiological properties (mass ratio, moisture) and biochemical properties (the content of the chlorophyll, carotenoids and the total polyphenol (TPC)) of Musa paradisiaca and Musa acuminata AA banana varieties peel, which were harvested in Tien Giang province, Vietnam at 03 stages of ripeness (I, III and VI in the color index 08 ripeness of banana). The results showed that, the ripening from I to VI maturity had a significant effected on the mass ratio, the moisture, the chlorophyll, the carotenoids and the TPC in 02 banana varieties peel and following different trends. The mass ratio and the moisture decreased, the chlorophyll and the carotenoids content recorded as opposite trends with degradation of chlorophyll and synthesis of carotenoids occured simultaneously, the most specially, the TPC increased and reached to maximum value at III maturity and decreased after. Results suggest that two banana varieties peel could be effectively used for extraction of polyphenol compounds in the future.","PeriodicalId":34742,"journal":{"name":"Ho Chi Minh City Open University Journal of Science Engineering and Technology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78354975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-29DOI: 10.46223/hcmcoujs.tech.en.12.1.1790.2022
T. Lao, L. Thuy
The deregulation of microRNA-140 (miRNA-140) has been reported to be involved in human pathogenesis of Osteoarthritis (OA). However, in Vietnam, to our knowledge, there are limit study of evaluation of miRNA-140 expression. The commercial kits for the quantitation of miRNA-140 are more expensive. Thus, for the aim to establish the less cost assay for quantitation of miRNAs in developing countries, we designed the set of specific primers to quantify the expression of microRNA for further application on the local population. The sequence of hsa-miR-140-3p was input from miRNA database using the accession number of MI0000456. Following bioinformatics tools, including RNAfold Web Server, BioEdit, IDT Oligo analyzer, were used to design the set of specific primers. As the result, we successfully designed the set of specific primers, including stem-loop primer, named as StL-miR-140-3p, which specific to target sequence of hsa-miR-140-3p, and specific forward primer and reversed primer, named as StL-F-miR-140-3p, and StL-F-miR-140-3p, respectively, which designed for target to the sequence of hsa-miR-140-3p. For further study, it is required to conduct the assay, which based on those designed primers, on the clinical samples to evaluate the specificity, sensitivity, limit of detection, as well as carried on the local population.
据报道，microRNA-140 (miRNA-140)的失调与人类骨关节炎(OA)的发病有关。然而，据我们所知，在越南，对miRNA-140表达评价的研究有限。用于miRNA-140定量的商用试剂盒更昂贵。因此，为了在发展中国家建立成本更低的mirna定量分析方法，我们设计了一套特定的引物来定量microRNA的表达，以便在当地人群中进一步应用。从miRNA数据库中输入hsa-miR-140-3p序列，登录号为MI0000456。采用RNAfold Web Server、BioEdit、IDT Oligo分析仪等生物信息学工具设计特异性引物。因此，我们成功设计了一组特异性引物，包括针对hsa-miR-140-3p的靶序列的茎环引物StL-miR-140-3p，以及针对hsa-miR-140-3p序列的特异性正向引物StL-F-miR-140-3p和特异性反向引物StL-F-miR-140-3p。为了进一步研究，需要根据设计的引物在临床样品上进行检测，评估特异性、敏感性、检出限，并在当地人群中进行。
{"title":"The in silico design of stem-loop Real-time PCR for detection of hsa-miR-140-3p expression on human Osteoarthritis","authors":"T. Lao, L. Thuy","doi":"10.46223/hcmcoujs.tech.en.12.1.1790.2022","DOIUrl":"https://doi.org/10.46223/hcmcoujs.tech.en.12.1.1790.2022","url":null,"abstract":"The deregulation of microRNA-140 (miRNA-140) has been reported to be involved in human pathogenesis of Osteoarthritis (OA). However, in Vietnam, to our knowledge, there are limit study of evaluation of miRNA-140 expression. The commercial kits for the quantitation of miRNA-140 are more expensive. Thus, for the aim to establish the less cost assay for quantitation of miRNAs in developing countries, we designed the set of specific primers to quantify the expression of microRNA for further application on the local population. The sequence of hsa-miR-140-3p was input from miRNA database using the accession number of MI0000456. Following bioinformatics tools, including RNAfold Web Server, BioEdit, IDT Oligo analyzer, were used to design the set of specific primers. As the result, we successfully designed the set of specific primers, including stem-loop primer, named as StL-miR-140-3p, which specific to target sequence of hsa-miR-140-3p, and specific forward primer and reversed primer, named as StL-F-miR-140-3p, and StL-F-miR-140-3p, respectively, which designed for target to the sequence of hsa-miR-140-3p. For further study, it is required to conduct the assay, which based on those designed primers, on the clinical samples to evaluate the specificity, sensitivity, limit of detection, as well as carried on the local population.","PeriodicalId":34742,"journal":{"name":"Ho Chi Minh City Open University Journal of Science Engineering and Technology","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83140308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pineapple and coconut are part of the largest commodities in Indonesia. In this study, extracts of pineapple skin and coconut water were used as a novel fermentation substrate in bioethanol production by Saccharomyces cerevisiae. Those were used as raw materials contain sugar, which making it a potential medium for bioethanol fermentation. The method used was a milling process to extract sugar into the fermentation medium, so that ethanol can be directly produced from the juice. While coconut water as a mixture during the fermentation process. The fermented bioethanol was then purified by distillation process to obtain pure bioethanol. The the sugar content of the ingredients, the operating condition (pH, time of fermentation) and the addition of nitrogen source were studied. Saccharomyces cerevisiae was used as a nitrogen source with variations of 14 and 16g/L with fermentation operating conditions for 40 and 44 hours and pH used were 04 and 05. The sugar content of pineapple skin extracts, coconut water, and the mixture of the two were 4.26; 4.19; and 4.21g/L, respectively. The largest ethanol content produced was 38.42% with 16g/L of nitrogen sources for 44 hours at pH 5. From these results, the mixture of pineapple skin extract and coconut water is proven to be used as a novel fermentation substrate for bioethanol production.
{"title":"A novel fermentation substrate in bioethanol production by saccharomyces cerevisiae","authors":"Fuadiyah Anissatul Dinana, Siswanto Puspitarini Anggun","doi":"10.46223/hcmcoujs.tech.en.12.1.1901.2022","DOIUrl":"https://doi.org/10.46223/hcmcoujs.tech.en.12.1.1901.2022","url":null,"abstract":"Pineapple and coconut are part of the largest commodities in Indonesia. In this study, extracts of pineapple skin and coconut water were used as a novel fermentation substrate in bioethanol production by Saccharomyces cerevisiae. Those were used as raw materials contain sugar, which making it a potential medium for bioethanol fermentation. The method used was a milling process to extract sugar into the fermentation medium, so that ethanol can be directly produced from the juice. While coconut water as a mixture during the fermentation process. The fermented bioethanol was then purified by distillation process to obtain pure bioethanol. The the sugar content of the ingredients, the operating condition (pH, time of fermentation) and the addition of nitrogen source were studied. Saccharomyces cerevisiae was used as a nitrogen source with variations of 14 and 16g/L with fermentation operating conditions for 40 and 44 hours and pH used were 04 and 05. The sugar content of pineapple skin extracts, coconut water, and the mixture of the two were 4.26; 4.19; and 4.21g/L, respectively. The largest ethanol content produced was 38.42% with 16g/L of nitrogen sources for 44 hours at pH 5. From these results, the mixture of pineapple skin extract and coconut water is proven to be used as a novel fermentation substrate for bioethanol production.","PeriodicalId":34742,"journal":{"name":"Ho Chi Minh City Open University Journal of Science Engineering and Technology","volume":"115 11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90231418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：