Pub Date : 2025-12-25DOI: 10.13702/j.1000-0607.20241154
Ke-Xin Sun, Yan-Jie Bai, Yan Wang, Yu-Qian Xiao, Jun Wan, Hui-Li Feng, Wen-Jing Li, An Liu, Hang-Yao Zhang, Yan-Jie Li
Objectives: To observe the effect of Tongdu Xingshen (unblocking the Governor Vessel and refreshing the spirit) needling on hypoxia-induced factor-1α/pyruvate dehydrogenase kinase 1 (HIF-1α/PDK1) pathway and hippocampal glycolysis level in rats with cerebral ischemia-reperfusion injury (CIRI), so as to explore its mechanisms underlying improvement of learning and memory ability.
Methods: SD rats were randomly divided into sham operation, model, acupuncture and acupuncture + inhibitor groups (n=12 in each group). The CIRI model was established by middle cerebral artery occlusion/reperfusion (MCAO/R). EA (1 Hz/20 Hz) was applied to "Shenting" (GV24) and "Baihui" (GV20) for 30 min, once daily for 14 consecutive days. In the acupuncture + inhibitor group, 2ME2 (5 mg/kg) was injected intraperitoneally 30 min before modeling. Learning and memory ability were evaluated by using open field test and new object recognition experiment. TTC staining was used to detect cerebral infarction volume. The pathological changes of hippocampus were observed by HE staining. The contents of lactic acid and ATP in hippocampal tissue were detected by colorimetric assay kit. The protein and mRNA expression levels of HIF-1α, PDK1, hexokinase 2 (HK2), phosphofructokinase 1 (PFK1) and pyruvate kinase M2 (PKM2) in hippocampal tissues on the ischemic side were detected by Western blot and qPCR, respectively.
Results: Compared with the sham operation group, the rats in the model group had a significant decrease in the proportion of central distance, the number of crossing platform and the new object preference index (P<0.01), and a significant increase in percentage of cerebral infarction volume (P<0.01). The content of lactic acid in hippocampus, the protein and mRNA expression of HIF-1α, PDK1, HK2, PFK1 and PKM2 were increased significantly (P<0.01), while the content of ATP was decreased (P<0.01) in the model group compared with those in the sham operation group. Following the interventions, modeling induced increase and decrease of the indexes mentioned above were all reversed in the acupuncture group (P<0.01), and the therapeutic effects of acupuncture + inhibitor were better than those of acupuncture alone (P<0.01, P<0.05). H.E. staining showed disordered cell arrangement, abnormal morphology, and evident nuclear rupture and nuclear pyknosis in the hippocampus tissue in the model group, which was relatively milder in the two intervention groups.
Conclusions: Tongdu Xingshen needling can improve the learning and memory function of CIRI rats, which may be associated with its function in down-regulating the expression of HIF-1α/PDK1 signaling pathway, inhibiting anaerobic glycolysis in the hippocampus, reducing lactic acid accumulation and increasing ATP production.
{"title":"[Tongdu Xingshen needling improves post-stroke cognitive impairment by regulating glycolysis in rats based on the HIF-1α/PDK1 pathway].","authors":"Ke-Xin Sun, Yan-Jie Bai, Yan Wang, Yu-Qian Xiao, Jun Wan, Hui-Li Feng, Wen-Jing Li, An Liu, Hang-Yao Zhang, Yan-Jie Li","doi":"10.13702/j.1000-0607.20241154","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20241154","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of Tongdu Xingshen (unblocking the Governor Vessel and refreshing the spirit) needling on hypoxia-induced factor-1α/pyruvate dehydrogenase kinase 1 (HIF-1α/PDK1) pathway and hippocampal glycolysis level in rats with cerebral ischemia-reperfusion injury (CIRI), so as to explore its mechanisms underlying improvement of learning and memory ability.</p><p><strong>Methods: </strong>SD rats were randomly divided into sham operation, model, acupuncture and acupuncture + inhibitor groups (<i>n</i>=12 in each group). The CIRI model was established by middle cerebral artery occlusion/reperfusion (MCAO/R). EA (1 Hz/20 Hz) was applied to \"Shenting\" (GV24) and \"Baihui\" (GV20) for 30 min, once daily for 14 consecutive days. In the acupuncture + inhibitor group, 2ME2 (5 mg/kg) was injected intraperitoneally 30 min before modeling. Learning and memory ability were evaluated by using open field test and new object recognition experiment. TTC staining was used to detect cerebral infarction volume. The pathological changes of hippocampus were observed by HE staining. The contents of lactic acid and ATP in hippocampal tissue were detected by colorimetric assay kit. The protein and mRNA expression levels of HIF-1α, PDK1, hexokinase 2 (HK2), phosphofructokinase 1 (PFK1) and pyruvate kinase M2 (PKM2) in hippocampal tissues on the ischemic side were detected by Western blot and qPCR, respectively.</p><p><strong>Results: </strong>Compared with the sham operation group, the rats in the model group had a significant decrease in the proportion of central distance, the number of crossing platform and the new object preference index (<i>P</i><0.01), and a significant increase in percentage of cerebral infarction volume (<i>P</i><0.01). The content of lactic acid in hippocampus, the protein and mRNA expression of HIF-1α, PDK1, HK2, PFK1 and PKM2 were increased significantly (<i>P</i><0.01), while the content of ATP was decreased (<i>P</i><0.01) in the model group compared with those in the sham operation group. Following the interventions, modeling induced increase and decrease of the indexes mentioned above were all reversed in the acupuncture group (<i>P</i><0.01), and the therapeutic effects of acupuncture + inhibitor were better than those of acupuncture alone (<i>P</i><0.01, <i>P</i><0.05). H.E. staining showed disordered cell arrangement, abnormal morphology, and evident nuclear rupture and nuclear pyknosis in the hippocampus tissue in the model group, which was relatively milder in the two intervention groups.</p><p><strong>Conclusions: </strong>Tongdu Xingshen needling can improve the learning and memory function of CIRI rats, which may be associated with its function in down-regulating the expression of HIF-1α/PDK1 signaling pathway, inhibiting anaerobic glycolysis in the hippocampus, reducing lactic acid accumulation and increasing ATP production.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 12","pages":"1387-1395"},"PeriodicalIF":0.0,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145828593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-25DOI: 10.13702/j.1000-0607.20241208
Ting-Wei Quan, Tian-Rong Liao, Ruo-Xian Bi, Jia-Yu Su, Hong-Zhen Tang
<p><strong>Objectives: </strong>To observe the effect of acupuncture on the expressions of transforming growth factor β(TGF-β)/Smad homolog 3(Smad 3)signaling pathway and autophagy-related factors in obese rats induced by a high-fat diet, so as to explore its possible mechanisms in improving obesity.</p><p><strong>Methods: </strong>Male SD rats were randomly divided into normal, model, medication and acupuncture groups, with 10 rats in each group.The obesity model was established by feeding the rats with a high-fat diet.Rats of the acupuncture group received acupuncture at "Guanyuan"(CV4) "Zhongwan"(CV12) and bilateral "Tianshu"(ST25) etc., for 30 min, once daily for 4 weeks. Rats in the medication group received gavage of orlistat solution (32.4 mg·kg<sup>-1</sup>·d<sup>-1</sup>), once daily for 4 weeks.The rat's body weight was measured for calculating the Lee's index.The weights of epididymal fat and perirenal fat were measured.The fasting blood glucose (FBG) and insulin levels were measured, and the homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated. Additionally, intraperitoneal glucose tolerance and insulin tolerance tests were performed.The serum levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were measured. HE staining was used to observe the morphology and structure of liver and epididymal adipose tissue, and oil red O staining was used to observe the lipid droplets in liver tissue.The mRNA and protein expression levels of TGF-β, TGF-β receptor 1 (TGF-βR1), Smad3, autophagy related protein Beclin-1, ubiquitin binding protein p62, and microtubule-associated protein 1 light chain 3(LC3) in epididymal adipose tissue were detected by real-time fluorescence quantitative PCR and Western blot, respectively.</p><p><strong>Results: </strong>Compared with the normal group, the body weight, Lee's index, perirenal and epididymal fat mass, serum TC, TG and LDL content, fasting insulin level, and HOMA-IR of rats in the model group were increased (<i>P</i><0.01), the blood glucose levels were also increased at 30, 60, and 120 min after glucose and insulin injection (<i>P</i><0.01, <i>P</i><0.05), while the HDL content was decreased (<i>P</i><0.01). Compared with the model group, the body weight, Lee's index, perirenal and epididymal fat mass, serum TC, TG and LDL content, and HOMA-IR of the medication and acupuncture groups were decreased (<i>P</i><0.01, <i>P</i><0.05), and the serum HDL content of the acupuncture group was increased (<i>P</i><0.01). Compared with the normal group, the mRNA expression levels of TGF-β, TGF-βR1, Smad3, LC3, and Beclin-1, the protein expression levels of TGF-β, TGF-βR1, p-Smad3/Smad3, LC3-Ⅱ/LC3- Ⅰ, and Beclin-1 in the epididymal adipose tissue of rats in the model group were significantly increased (<i>P</i><0.01, <i>P</i><0.05), while the mRNA and protein expression levels of p62 were decreased (<i>P</i><0.01). Compared w
{"title":"[Effect of acupuncture on the transforming growth factor β/Smad homolog 3 signaling pathway and autophagy in obese rats].","authors":"Ting-Wei Quan, Tian-Rong Liao, Ruo-Xian Bi, Jia-Yu Su, Hong-Zhen Tang","doi":"10.13702/j.1000-0607.20241208","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20241208","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of acupuncture on the expressions of transforming growth factor β(TGF-β)/Smad homolog 3(Smad 3)signaling pathway and autophagy-related factors in obese rats induced by a high-fat diet, so as to explore its possible mechanisms in improving obesity.</p><p><strong>Methods: </strong>Male SD rats were randomly divided into normal, model, medication and acupuncture groups, with 10 rats in each group.The obesity model was established by feeding the rats with a high-fat diet.Rats of the acupuncture group received acupuncture at \"Guanyuan\"(CV4) \"Zhongwan\"(CV12) and bilateral \"Tianshu\"(ST25) etc., for 30 min, once daily for 4 weeks. Rats in the medication group received gavage of orlistat solution (32.4 mg·kg<sup>-1</sup>·d<sup>-1</sup>), once daily for 4 weeks.The rat's body weight was measured for calculating the Lee's index.The weights of epididymal fat and perirenal fat were measured.The fasting blood glucose (FBG) and insulin levels were measured, and the homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated. Additionally, intraperitoneal glucose tolerance and insulin tolerance tests were performed.The serum levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were measured. HE staining was used to observe the morphology and structure of liver and epididymal adipose tissue, and oil red O staining was used to observe the lipid droplets in liver tissue.The mRNA and protein expression levels of TGF-β, TGF-β receptor 1 (TGF-βR1), Smad3, autophagy related protein Beclin-1, ubiquitin binding protein p62, and microtubule-associated protein 1 light chain 3(LC3) in epididymal adipose tissue were detected by real-time fluorescence quantitative PCR and Western blot, respectively.</p><p><strong>Results: </strong>Compared with the normal group, the body weight, Lee's index, perirenal and epididymal fat mass, serum TC, TG and LDL content, fasting insulin level, and HOMA-IR of rats in the model group were increased (<i>P</i><0.01), the blood glucose levels were also increased at 30, 60, and 120 min after glucose and insulin injection (<i>P</i><0.01, <i>P</i><0.05), while the HDL content was decreased (<i>P</i><0.01). Compared with the model group, the body weight, Lee's index, perirenal and epididymal fat mass, serum TC, TG and LDL content, and HOMA-IR of the medication and acupuncture groups were decreased (<i>P</i><0.01, <i>P</i><0.05), and the serum HDL content of the acupuncture group was increased (<i>P</i><0.01). Compared with the normal group, the mRNA expression levels of TGF-β, TGF-βR1, Smad3, LC3, and Beclin-1, the protein expression levels of TGF-β, TGF-βR1, p-Smad3/Smad3, LC3-Ⅱ/LC3- Ⅰ, and Beclin-1 in the epididymal adipose tissue of rats in the model group were significantly increased (<i>P</i><0.01, <i>P</i><0.05), while the mRNA and protein expression levels of p62 were decreased (<i>P</i><0.01). Compared w","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 12","pages":"1421-1430"},"PeriodicalIF":0.0,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145828598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives: To investigate the effect of acupuncture on the expressions of cuproptosis marker proteins-ferredoxin 1 (FDX1) and lipoic acid synthase (LIAS) in the ischemic penumbra (IP) of rats with cerebral ischemia-reperfusion injury (CIRI).
Methods: SD rats were randomly divided into sham operation, model and acupuncture groups (n=15). The CIRI model was established by occlusion of the middle cerebral artery and reperfusion (MCAO/R). In the acupuncture group, "Dazhui" (GV14), "Baihui" (GV20) and "Shuigou" (GV26) were punctured with filiform needles for 30 min, once every 12 h for 7 times. Neurological function was assessed using modified neurological severity scores (mNSS). Cerebral infarction volume was measured by TTC staining. Copper ion content in IP tissue was determined by colorimetric method. Mitochondrial respiratory chain complexes (MRCC)Ⅰ and Ⅱ contents were detected by ELISA. Protein expressions of FDX1, LIAS, and heat shock protein 70 (HSP70) were analyzed by Western blot. Mitochondrial morphology and structure in the IP region were observed via transmission electron microscopy (TEM).
Results: Compared with the sham operation group, the mNSS score (P<0.01), the volume percentage of cerebral infarction (P<0.000 1), copper ion in IP (P<0.01), MRCCⅠ and Ⅱ contents (P<0.000 1) and HSP70 protein expression (P<0.001) were significantly increased, while the expression of FDX1 (P<0.01) and LIAS (P<0.001) were decreased in the model group. Compared with the model group, the mNSS score (P<0.05), the volume percentage of cerebral infarction (P<0.001), the contents of IP copper ion (P<0.001), MRCCⅠ and Ⅱ contents (P<0.01, P<0.000 1) and HSP70 protein expression decreased (P<0.01), whereas the protein expressions of FDX1 and LIAS were increased (P<0.05) in the acupuncture group. TEM results showed that the outer membrane of mitochondria swelled and ruptured, the inner cristae ruptured and disappeared, and the density of the mitochondrial matrix decreased in the model group, which were relatively milder in the acupuncture group.
Conclusions: Acupuncture intervention can reduce the volume of cerebral infarction and improve the symptoms of neurological deficit in CIRI rats, and its mechanism may be related to reducing the loss of FDX1 and LIAS proteins and inhibiting the surge of HSP70 protein, thus preventing cuproptosis in brain cells.
{"title":"[Effect of acupuncture on cuproptosis in the ischemic penumbra of rats with cerebral ischemia-reperfusion injury].","authors":"Qian Li, Lu-Bo Xiao, Xiao-Jing Luo, Chu-Ning Tian, Shuan Hu, Jia-Li Peng, Hao-Mei Tian, Xiao-Ye Lu, Hong Zhang","doi":"10.13702/j.1000-0607.20250429","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20250429","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effect of acupuncture on the expressions of cuproptosis marker proteins-ferredoxin 1 (FDX1) and lipoic acid synthase (LIAS) in the ischemic penumbra (IP) of rats with cerebral ischemia-reperfusion injury (CIRI).</p><p><strong>Methods: </strong>SD rats were randomly divided into sham operation, model and acupuncture groups (<i>n</i>=15). The CIRI model was established by occlusion of the middle cerebral artery and reperfusion (MCAO/R). In the acupuncture group, \"Dazhui\" (GV14), \"Baihui\" (GV20) and \"Shuigou\" (GV26) were punctured with filiform needles for 30 min, once every 12 h for 7 times. Neurological function was assessed using modified neurological severity scores (mNSS). Cerebral infarction volume was measured by TTC staining. Copper ion content in IP tissue was determined by colorimetric method. Mitochondrial respiratory chain complexes (MRCC)Ⅰ and Ⅱ contents were detected by ELISA. Protein expressions of FDX1, LIAS, and heat shock protein 70 (HSP70) were analyzed by Western blot. Mitochondrial morphology and structure in the IP region were observed via transmission electron microscopy (TEM).</p><p><strong>Results: </strong>Compared with the sham operation group, the mNSS score (<i>P</i><0.01), the volume percentage of cerebral infarction (<i>P</i><0.000 1), copper ion in IP (<i>P</i><0.01), MRCCⅠ and Ⅱ contents (<i>P</i><0.000 1) and HSP70 protein expression (<i>P</i><0.001) were significantly increased, while the expression of FDX1 (<i>P</i><0.01) and LIAS (<i>P</i><0.001) were decreased in the model group. Compared with the model group, the mNSS score (<i>P</i><0.05), the volume percentage of cerebral infarction (<i>P</i><0.001), the contents of IP copper ion (<i>P</i><0.001), MRCCⅠ and Ⅱ contents (<i>P</i><0.01, <i>P</i><0.000 1) and HSP70 protein expression decreased (<i>P</i><0.01), whereas the protein expressions of FDX1 and LIAS were increased (<i>P</i><0.05) in the acupuncture group. TEM results showed that the outer membrane of mitochondria swelled and ruptured, the inner cristae ruptured and disappeared, and the density of the mitochondrial matrix decreased in the model group, which were relatively milder in the acupuncture group.</p><p><strong>Conclusions: </strong>Acupuncture intervention can reduce the volume of cerebral infarction and improve the symptoms of neurological deficit in CIRI rats, and its mechanism may be related to reducing the loss of FDX1 and LIAS proteins and inhibiting the surge of HSP70 protein, thus preventing cuproptosis in brain cells.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 12","pages":"1396-1403"},"PeriodicalIF":0.0,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145828607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-25DOI: 10.13702/j.1000-0607.20241069
Meng-Ni Hu, Jun Ma, Jia-Yong Wei, Yao Wang, Ya-Nan Li, Shu-Xiu Zhu
Objectives: To observe the effect of electroacupuncture(EA) at "Fengfu" (GV16), "Taichong" (LR3) and "Zusanli" (ST36) on the behavior and insulin receptor substrate 1 (IRS-1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway mediated by the insulin-like growth factor 1 receptor (IGF-1R) in the substantia nigra of the midbrain in mice model with Parkinson's disease (PD), so as to explore the mechanism of EA in the prevention and treatment of PD.
Methods: C57BL/6 mice were randomly divided into a control group, a model group, and an EA group, with 10 mice in each group. A PD mouse model was established by intraperitoneal injection of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP, 30 mg·kg-1·d-1) for 7 consecutive days. The mice in the EA group were given EA at GV16, LR3, and ST36 for 30 min each time, once a day for 12 consecutive days. The open field test was used to observe the behavioral changes of the mice in each group before and after treatment. Immunohistochemistry was used to detect the expression level of tyrosine hydroxylase (TH) in the substantia nigra region of the midbrain. Real-time fluorescence quantitative PCR and Western blot were used to detect the mRNA expression levels of IGF-1R, IRS-1, PI3K, and AKT, and protein expressions of IGF-1R, IRS-1, p-PI3K, and p-AKT in the substantia nigra of the mice, respectively. ELISA was used to detect the contents of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the substantia nigra of the midbrain.
Results: Compared with the control group, mice in the model group showed obvious motor dysfunction. The total distance of spontaneous movement, the average speed, and the duration of movement, the positive expression level of TH in the substantia nigra of the midbrain, and the mRNA expression levels of IGF-1R, PI3K, and AKT, and protein expressions of IGF-1R, p-PI3K/PI3K and p-AKT/AKT ratio in the substantia nigra decreased (P<0.01, P<0.05), while the mRNA and protein expression levels of IRS-1, the contents of the inflammatory factors TNF-α and IL-1β increased (P<0.05, P<0.01). Compared with the model group, all the above indexes of the PD mice after EA treatment were all reversed (P<0.01, P<0.05).
Conclusions: EA at GV16, LR3, and ST36 can effectively improve the bradykinesia of PD model mice induced by MPTP, increase the expression level of TH in the substantia nigra, and improve the insulin signaling dysfunction. Its mechanism may be related to the regulation of the IGF-1R/IRS-1/PI3K/AKT signaling pathway and the subsequent inhibition of the neuroinflammatory response.
{"title":"[Effect of electroacupuncture on insulin signaling in the substantia nigra of the midbrain in mice with Parkinson's disease].","authors":"Meng-Ni Hu, Jun Ma, Jia-Yong Wei, Yao Wang, Ya-Nan Li, Shu-Xiu Zhu","doi":"10.13702/j.1000-0607.20241069","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20241069","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture(EA) at \"Fengfu\" (GV16), \"Taichong\" (LR3) and \"Zusanli\" (ST36) on the behavior and insulin receptor substrate 1 (IRS-1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway mediated by the insulin-like growth factor 1 receptor (IGF-1R) in the substantia nigra of the midbrain in mice model with Parkinson's disease (PD), so as to explore the mechanism of EA in the prevention and treatment of PD.</p><p><strong>Methods: </strong>C57BL/6 mice were randomly divided into a control group, a model group, and an EA group, with 10 mice in each group. A PD mouse model was established by intraperitoneal injection of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP, 30 mg·kg<sup>-1</sup>·d<sup>-1</sup>) for 7 consecutive days. The mice in the EA group were given EA at GV16, LR3, and ST36 for 30 min each time, once a day for 12 consecutive days. The open field test was used to observe the behavioral changes of the mice in each group before and after treatment. Immunohistochemistry was used to detect the expression level of tyrosine hydroxylase (TH) in the substantia nigra region of the midbrain. Real-time fluorescence quantitative PCR and Western blot were used to detect the mRNA expression levels of IGF-1R, IRS-1, PI3K, and AKT, and protein expressions of IGF-1R, IRS-1, p-PI3K, and p-AKT in the substantia nigra of the mice, respectively. ELISA was used to detect the contents of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the substantia nigra of the midbrain.</p><p><strong>Results: </strong>Compared with the control group, mice in the model group showed obvious motor dysfunction. The total distance of spontaneous movement, the average speed, and the duration of movement, the positive expression level of TH in the substantia nigra of the midbrain, and the mRNA expression levels of IGF-1R, PI3K, and AKT, and protein expressions of IGF-1R, p-PI3K/PI3K and p-AKT/AKT ratio in the substantia nigra decreased (<i>P</i><0.01, <i>P</i><0.05), while the mRNA and protein expression levels of IRS-1, the contents of the inflammatory factors TNF-α and IL-1β increased (<i>P</i><0.05, <i>P</i><0.01). Compared with the model group, all the above indexes of the PD mice after EA treatment were all reversed (<i>P</i><0.01, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>EA at GV16, LR3, and ST36 can effectively improve the bradykinesia of PD model mice induced by MPTP, increase the expression level of TH in the substantia nigra, and improve the insulin signaling dysfunction. Its mechanism may be related to the regulation of the IGF-1R/IRS-1/PI3K/AKT signaling pathway and the subsequent inhibition of the neuroinflammatory response.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 12","pages":"1404-1411"},"PeriodicalIF":0.0,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145828639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-25DOI: 10.13702/j.1000-0607.20241170
Yang-Yang Ye, Xiao-Xiao Liu, Wei Yi, Jian Sun, Hai-Hua Zhang, Zhi-Xing Li
<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) at "Sanyinjiao" (SP6) and "Zusanli" (ST36) on the expressions of peroxisome proliferator-activated γ receptor (PPAR-γ), nuclear factor-κB (NF-κB) and glucose transporter 4 (GLUT4) in skeletal muscle of Zucker Diabetic Fatty (ZDF) rats with type 2 diabetes mellitus (T2DM), so as to explore its possible mechanism in improving insulin resistance (IR).</p><p><strong>Methods: </strong>Eighteen male 2-month-old ZDF (Lepr<sup>fa/fa</sup>) rats were fed with a high-fat diet for 4 weeks to establish a diabetic model. After the model was confirmed, the rats were randomly divided into model, EA and medication groups, with 6 rats in each group. Additionally, 6 Zucker lean rats (Lepr<sup>+/fa</sup>) of the same age were used as the control group. EA (15 Hz, 1 mA) was applied to bilateral SP6 and ST36 for 20 min;the medication group was administered pioglitazone solution (10 mg/kg) via gastric gavage;both groups were treated once daily, 5 d a week for 4 weeks. Fasting body weight (FBW) was measured weekly, fasting blood glucose (FBG) was measured the day before sampling. The levels of serum fasting insulin (FINS), free fatty acid (FFA), low density lipoprotein (LDL), total cholesterol (TC), triglyceride (TG), tumor necrosis factor-α (TNF-α), C peptide and C reactive protein (CRP) were detected by ELISA, and the homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated. HE staining and oil red O staining were used to observe the pathological changes and lipid droplet accumulation of skeletal muscle in rats respectively. The protein expression levels of PPAR-γ, NF-κB and phosphorylated (p) -NF-κB in skeletal muscle tissue of rats were detected by Western blot. The positive expressions of TNF-α and GLUT4 in skeletal muscle of rats were detected by immunofluorescence.</p><p><strong>Results: </strong>Compared with the control group, the FBW, FBG, HOMA-IR index, the serum levels of FINS, FFA, LDL, TG, TC, TNF-α, C-peptide and CRP, NF-κB phosphorylation level, as well as the positive expression of TNF-α and p-NF-κB/NF-κB in skeletal muscle were increased (<i>P</i><0.01), while the PPAR-γ expression and the positive expression of GLUT4 in skeletal muscle were decreased (<i>P</i><0.01) in the model group. Oil red O staining showed a large number of bright red lipid droplets and serious lipid accumulation in skeletal muscle cells, HE staining showed that the skeletal muscle fibers were broken in the model group. Compared with the model group, the FBW of the medication group, the expression levels of PPAR-γ and GLUT4 of both EA and medication groups were increased (<i>P</i><0.01). Conversely, the levels of FBG, HOMA-IR index, FINS, FFA, LDL, TG, TC, TNF-α, C-peptide and CRP, the phosphorylation level of NF-κB protein and TNF-α expression were decreased (<i>P</i><0.05, <i>P</i><0.01) in the EA and medication groups. The oil red O staining showed that the lipid droplets
{"title":"[Electroacupuncture ameliorates skeletal muscle insulin resistance in diabetic rats via PPAR-γ/NF-κB signaling pathway].","authors":"Yang-Yang Ye, Xiao-Xiao Liu, Wei Yi, Jian Sun, Hai-Hua Zhang, Zhi-Xing Li","doi":"10.13702/j.1000-0607.20241170","DOIUrl":"https://doi.org/10.13702/j.1000-0607.20241170","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of electroacupuncture (EA) at \"Sanyinjiao\" (SP6) and \"Zusanli\" (ST36) on the expressions of peroxisome proliferator-activated γ receptor (PPAR-γ), nuclear factor-κB (NF-κB) and glucose transporter 4 (GLUT4) in skeletal muscle of Zucker Diabetic Fatty (ZDF) rats with type 2 diabetes mellitus (T2DM), so as to explore its possible mechanism in improving insulin resistance (IR).</p><p><strong>Methods: </strong>Eighteen male 2-month-old ZDF (Lepr<sup>fa/fa</sup>) rats were fed with a high-fat diet for 4 weeks to establish a diabetic model. After the model was confirmed, the rats were randomly divided into model, EA and medication groups, with 6 rats in each group. Additionally, 6 Zucker lean rats (Lepr<sup>+/fa</sup>) of the same age were used as the control group. EA (15 Hz, 1 mA) was applied to bilateral SP6 and ST36 for 20 min;the medication group was administered pioglitazone solution (10 mg/kg) via gastric gavage;both groups were treated once daily, 5 d a week for 4 weeks. Fasting body weight (FBW) was measured weekly, fasting blood glucose (FBG) was measured the day before sampling. The levels of serum fasting insulin (FINS), free fatty acid (FFA), low density lipoprotein (LDL), total cholesterol (TC), triglyceride (TG), tumor necrosis factor-α (TNF-α), C peptide and C reactive protein (CRP) were detected by ELISA, and the homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated. HE staining and oil red O staining were used to observe the pathological changes and lipid droplet accumulation of skeletal muscle in rats respectively. The protein expression levels of PPAR-γ, NF-κB and phosphorylated (p) -NF-κB in skeletal muscle tissue of rats were detected by Western blot. The positive expressions of TNF-α and GLUT4 in skeletal muscle of rats were detected by immunofluorescence.</p><p><strong>Results: </strong>Compared with the control group, the FBW, FBG, HOMA-IR index, the serum levels of FINS, FFA, LDL, TG, TC, TNF-α, C-peptide and CRP, NF-κB phosphorylation level, as well as the positive expression of TNF-α and p-NF-κB/NF-κB in skeletal muscle were increased (<i>P</i><0.01), while the PPAR-γ expression and the positive expression of GLUT4 in skeletal muscle were decreased (<i>P</i><0.01) in the model group. Oil red O staining showed a large number of bright red lipid droplets and serious lipid accumulation in skeletal muscle cells, HE staining showed that the skeletal muscle fibers were broken in the model group. Compared with the model group, the FBW of the medication group, the expression levels of PPAR-γ and GLUT4 of both EA and medication groups were increased (<i>P</i><0.01). Conversely, the levels of FBG, HOMA-IR index, FINS, FFA, LDL, TG, TC, TNF-α, C-peptide and CRP, the phosphorylation level of NF-κB protein and TNF-α expression were decreased (<i>P</i><0.05, <i>P</i><0.01) in the EA and medication groups. The oil red O staining showed that the lipid droplets","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 12","pages":"1412-1420"},"PeriodicalIF":0.0,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145828646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives: To explore the effect of acupuncture intervention combined with lateral approach sciatic nerve block (LSNB) on postoperative analgesia in patients after total knee arthroplasty (TKA).
Methods: A total of 90 patients scheduled for TKA between May 2022 and May 2024 were selected as the study subjects. They were randomly divided into a control group and an observation group using the sealed envelope method, with 45 patients(one case dropped out in each group)in each group. The control group received LSNB for postoperative analgesia, while the observation group received acupuncture intervention (manual acupuncture stimulation of Taichong [LR3], Kunlun [BL60], Taixi [KI3], Shenmai [BL62] and Sanyinjiao [SP6] on the affected side, and Houxi [SI3], Chize [LU5], Quchi [LI11] and Shousanli [LI10] on the healthy side, 30 min per session, once daily for 7 days) in addition to LSNB. Postoperative rescue analgesic usage and sleep monitoring results were recorded. Resting and passive functional exercise-induced limb pain intensity was assessed using the visual analog scale (VAS) at 2 hours (T0), 6 hours (T1), 24 hours (T2), 48 hours (T3), 72 hours (T4), and 7 days (T5) postoperatively. Recovery quality was evaluated using the 15-item quality of recovery scale (QoR-15) at T0, T5, 1 month (T6), and 3 months (T7) postoperatively. The safety of the analgesic regimens was also assessed.
Results: The frequency of rescue analgesic use and the total dosage consumed were significantly lower in the observation group than in the control group (P<0.05). There were no statistically significant differences between two groups in the sleep latency and N1 sleep stage proportion within 0-12 hours after operation. However, within 12-72 hours postoperatively, the observation group had fewer awakenings, a higher proportion of N3 deep sleep, and higher sleep efficiency than the control group (P<0.05). At T5, VAS scores at rest and during passive functional exercise were significantly lower in the observation group than in the control group (P<0.05). The incidence of adverse reactions was below 10% in both groups, with no statistically significant difference between the two groups . QoR-15 scores were significantly higher in the observation group than in the control group at T5 and T6 (P<0.05).
Conclusions: Compared with LSNB alone, acupuncture intervention combined with LSNB after TKA can reduce the need for rescue analgesia, relieve early postoperative pain, and improve the quality of sleep and postoperative recovery, and is safe.
{"title":"[Effect of acupuncture intervention combined with lateral approach sciatic nerve block on postoperative analgesia in patients after total knee arthroplasty].","authors":"Ji-Juan Lu, Zhi-Gang Zhang, Xing-Xing Duan, Jie-Ying Shen","doi":"10.13702/j.1000-0607.20250678","DOIUrl":"10.13702/j.1000-0607.20250678","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the effect of acupuncture intervention combined with lateral approach sciatic nerve block (LSNB) on postoperative analgesia in patients after total knee arthroplasty (TKA).</p><p><strong>Methods: </strong>A total of 90 patients scheduled for TKA between May 2022 and May 2024 were selected as the study subjects. They were randomly divided into a control group and an observation group using the sealed envelope method, with 45 patients(one case dropped out in each group)in each group. The control group received LSNB for postoperative analgesia, while the observation group received acupuncture intervention (manual acupuncture stimulation of Taichong [LR3], Kunlun [BL60], Taixi [KI3], Shenmai [BL62] and Sanyinjiao [SP6] on the affected side, and Houxi [SI3], Chize [LU5], Quchi [LI11] and Shousanli [LI10] on the healthy side, 30 min per session, once daily for 7 days) in addition to LSNB. Postoperative rescue analgesic usage and sleep monitoring results were recorded. Resting and passive functional exercise-induced limb pain intensity was assessed using the visual analog scale (VAS) at 2 hours (T<sub>0</sub>), 6 hours (T<sub>1</sub>), 24 hours (T<sub>2</sub>), 48 hours (T<sub>3</sub>), 72 hours (T<sub>4</sub>), and 7 days (T<sub>5</sub>) postoperatively. Recovery quality was evaluated using the 15-item quality of recovery scale (QoR-15) at T<sub>0</sub>, T<sub>5</sub>, 1 month (T<sub>6</sub>), and 3 months (T<sub>7</sub>) postoperatively. The safety of the analgesic regimens was also assessed.</p><p><strong>Results: </strong>The frequency of rescue analgesic use and the total dosage consumed were significantly lower in the observation group than in the control group (<i>P</i><0.05). There were no statistically significant differences between two groups in the sleep latency and N1 sleep stage proportion within 0-12 hours after operation. However, within 12-72 hours postoperatively, the observation group had fewer awakenings, a higher proportion of N3 deep sleep, and higher sleep efficiency than the control group (<i>P</i><0.05). At T<sub>5</sub>, VAS scores at rest and during passive functional exercise were significantly lower in the observation group than in the control group (<i>P</i><0.05). The incidence of adverse reactions was below 10% in both groups, with no statistically significant difference between the two groups . QoR-15 scores were significantly higher in the observation group than in the control group at T<sub>5</sub> and T<sub>6</sub> (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>Compared with LSNB alone, acupuncture intervention combined with LSNB after TKA can reduce the need for rescue analgesia, relieve early postoperative pain, and improve the quality of sleep and postoperative recovery, and is safe.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 12","pages":"1456-1462"},"PeriodicalIF":0.0,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145828624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-25DOI: 10.13702/j.1000-0607.20250421
Nuo Li, Xiao-Ning Li, Yong-Lie Zhao, Xiao-Qing Zhou
<p><strong>Objectives: </strong>To observe the effects on swallowing reflex latency (SRL), the distances of hyoid bone forward and upward movements in the patients with post-stroke dysphagia (PSD) based on videofluoroscopic swallow study (VFSS) after treated with scalp acupuncture combined with five points in the anterior cervical region.</p><p><strong>Methods: </strong>Sixty-six patients with PSD were randomly divided into a trial group (33 cases, 1 case dropped out) and a control group (33 cases, 1 case dropped out). The basic treatment with neurological medicine and with acupuncture for stroke (Neiguan [PC6], Sanyinjiao [SP6], Chize [LU5] and Weizhong [BL40] on the affected side) were provided in the two groups. Besides, in the trial group, scalp acupuncture was delivered and combined with five points in the anterior cervical region;and in the control group, acupuncture was applied to Shuigou (GV26), Lianquan (CV23), Tongli (HT5) and Zhaohai (KI6). The treatment was administered once daily, for 30 min each time;and 2 weeks of treatment were required, with the interval of 1 d after 6 interventions. Using VFSS, SRL and the distances of hyoid bone forward and upward movements were assessed before and after treatment in the 2 groups;and the changes in the scores of standardized swallowing assessment (SSA), water swallowing test (WST), and swallowing quality of life questionnaire (SWAL-QOL) were observed. The correlation was analyzed between VFSS indicators and scale assessment results.</p><p><strong>Results: </strong>Compared with those before treatment, SRL was shortened (<i>P</i><0.01), the distances of hyoid bone forward and upward movements increased (<i>P</i><0.01) in both groups. When compared with the control group after treatment, SRL was shorter (<i>P</i><0.01), the distances of hyoid bone forward and upward movements were longer (<i>P</i><0.01) in the trial group. SSA score was reduced in comparison with that before treatment in either group (<i>P</i><0.01), and the score in the trial group was lower than that of the control group (<i>P</i><0.01). The WST grade was better after treatment in both groups (<i>P</i><0.01), and the grade in the trial group was superior to that in the control group (<i>P</i><0.05). SWAL-QOL score was increased after treatment in both groups (<i>P</i><0.01), and the score in the trial group was higher than that of the control group (<i>P</i><0.01). The clinical effective rate was 93.75% (30/32) in the trial group, which was higher than that (81.25%, 26/32) in the control group (<i>P</i><0.05). The correlation analysis showed that the SSA score and WST grade were positively correlated with SRL before and after treatment (<i>P</i><0.01), and were negatively correlated with the distances of hyoid bone forward and upward movements (<i>P</i><0.01). During trial, no obvious adverse reactions occurred.</p><p><strong>Conclusions: </strong>Acupuncture at head points, combined with the five points in the anterior cervica
{"title":"[Effects of scalp acupuncture combined with five points in the anterior cervical region on patients with post-stroke dysphagia based on videofluoroscopic swallow study].","authors":"Nuo Li, Xiao-Ning Li, Yong-Lie Zhao, Xiao-Qing Zhou","doi":"10.13702/j.1000-0607.20250421","DOIUrl":"10.13702/j.1000-0607.20250421","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effects on swallowing reflex latency (SRL), the distances of hyoid bone forward and upward movements in the patients with post-stroke dysphagia (PSD) based on videofluoroscopic swallow study (VFSS) after treated with scalp acupuncture combined with five points in the anterior cervical region.</p><p><strong>Methods: </strong>Sixty-six patients with PSD were randomly divided into a trial group (33 cases, 1 case dropped out) and a control group (33 cases, 1 case dropped out). The basic treatment with neurological medicine and with acupuncture for stroke (Neiguan [PC6], Sanyinjiao [SP6], Chize [LU5] and Weizhong [BL40] on the affected side) were provided in the two groups. Besides, in the trial group, scalp acupuncture was delivered and combined with five points in the anterior cervical region;and in the control group, acupuncture was applied to Shuigou (GV26), Lianquan (CV23), Tongli (HT5) and Zhaohai (KI6). The treatment was administered once daily, for 30 min each time;and 2 weeks of treatment were required, with the interval of 1 d after 6 interventions. Using VFSS, SRL and the distances of hyoid bone forward and upward movements were assessed before and after treatment in the 2 groups;and the changes in the scores of standardized swallowing assessment (SSA), water swallowing test (WST), and swallowing quality of life questionnaire (SWAL-QOL) were observed. The correlation was analyzed between VFSS indicators and scale assessment results.</p><p><strong>Results: </strong>Compared with those before treatment, SRL was shortened (<i>P</i><0.01), the distances of hyoid bone forward and upward movements increased (<i>P</i><0.01) in both groups. When compared with the control group after treatment, SRL was shorter (<i>P</i><0.01), the distances of hyoid bone forward and upward movements were longer (<i>P</i><0.01) in the trial group. SSA score was reduced in comparison with that before treatment in either group (<i>P</i><0.01), and the score in the trial group was lower than that of the control group (<i>P</i><0.01). The WST grade was better after treatment in both groups (<i>P</i><0.01), and the grade in the trial group was superior to that in the control group (<i>P</i><0.05). SWAL-QOL score was increased after treatment in both groups (<i>P</i><0.01), and the score in the trial group was higher than that of the control group (<i>P</i><0.01). The clinical effective rate was 93.75% (30/32) in the trial group, which was higher than that (81.25%, 26/32) in the control group (<i>P</i><0.05). The correlation analysis showed that the SSA score and WST grade were positively correlated with SRL before and after treatment (<i>P</i><0.01), and were negatively correlated with the distances of hyoid bone forward and upward movements (<i>P</i><0.01). During trial, no obvious adverse reactions occurred.</p><p><strong>Conclusions: </strong>Acupuncture at head points, combined with the five points in the anterior cervica","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 11","pages":"1308-1316"},"PeriodicalIF":0.0,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-25DOI: 10.13702/j.1000-0607.20240916
Kai-Yang Xue, Hong-Yan Luo, Wei-Wei Zhang, Xiao-Xiao Lu, Tao Xu, Jin Cui
Objectives: To observe the clinical effect and safety of herbal cake-insulated moxibustion in the auxiliary treatment of carotid atherosclerosis (CAS) differentiated as interaction of phlegm and blood stasis.
Methods: Sixty-three patients with CAS differentiated as interaction of phlegm and blood stasis were randomly divided into an experimental group (33 cases, 4 cases dropped out) and a control group (30 cases, 3 cases dropped out). In the experimental group, herbal cake-insulated moxibustion was delivered in combination with oral administration with atorvastatin. In the control group, atorvastatin was administered orally. The duration of treatment in either group was composed of 12 weeks. Common carotid intima-media thickness (cIMT), the total area of carotid plaque, common carotid blood flow parameters, blood lipids contents (4 items) and traditional Chinese medicine (TCM) syndrome score were compared between the two groups before and after treatment. Blood routine and liver and kidney function were detected before and after treatment, and adverse events were recorded to evaluate safety.
Results: Compared with those before treatment, the bilateral cIMT and the contents of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in serum were reduced after treatment (P<0.05, P<0.001), the scores of the primary symptoms and secondary symptoms of TCM, as well as the total score were lower (P<0.001) in the two groups. The left cIMT and the contents of the serum TC, TG and LDL-C in the experimental group were lower than those of the control group (P<0.05, P<0.01), and the scores of the primary symptoms and secondary symptoms of TCM, as well as the total score were lower than those of the control group (P<0.001, P<0.01). After treatment, the bilateral peak systolic velocity (PSV) and end diastolic velocity (EDV) were increased in the experimental group (P<0.001), the right EDV was increased in the control group (P<0.001), and the bilateral PSV and EDV in the experimental group were higher than those of the control group (P<0.05, P<0.01, P<0.001). After treatment, there was no significant difference in the total area of carotid plaque between the two groups. After treatment, there were no significant abnormalities in blood routine and liver and kidney functions in both groups, and the safety was good.
Conclusions: On the basis of conventional medication, the herbal cake-insulated moxibustion as an auxiliary treatment, presents its synergistic effect on CAS of interaction of phlegm and blood stasis syndrome in the patients, and this therapy is safe in clinical application.
{"title":"[Herbal cake-insulated moxibustion in auxiliary treatment of carotid atherosclerosis with interaction of phlegm and blood stasis: a randomized controlled trial].","authors":"Kai-Yang Xue, Hong-Yan Luo, Wei-Wei Zhang, Xiao-Xiao Lu, Tao Xu, Jin Cui","doi":"10.13702/j.1000-0607.20240916","DOIUrl":"10.13702/j.1000-0607.20240916","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the clinical effect and safety of herbal cake-insulated moxibustion in the auxiliary treatment of carotid atherosclerosis (CAS) differentiated as interaction of phlegm and blood stasis.</p><p><strong>Methods: </strong>Sixty-three patients with CAS differentiated as interaction of phlegm and blood stasis were randomly divided into an experimental group (33 cases, 4 cases dropped out) and a control group (30 cases, 3 cases dropped out). In the experimental group, herbal cake-insulated moxibustion was delivered in combination with oral administration with atorvastatin. In the control group, atorvastatin was administered orally. The duration of treatment in either group was composed of 12 weeks. Common carotid intima-media thickness (cIMT), the total area of carotid plaque, common carotid blood flow parameters, blood lipids contents (4 items) and traditional Chinese medicine (TCM) syndrome score were compared between the two groups before and after treatment. Blood routine and liver and kidney function were detected before and after treatment, and adverse events were recorded to evaluate safety.</p><p><strong>Results: </strong>Compared with those before treatment, the bilateral cIMT and the contents of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in serum were reduced after treatment (<i>P<</i>0.05, <i>P<</i>0.001), the scores of the primary symptoms and secondary symptoms of TCM, as well as the total score were lower (<i>P<</i>0.001) in the two groups. The left cIMT and the contents of the serum TC, TG and LDL-C in the experimental group were lower than those of the control group (<i>P</i><0.05, <i>P</i><0.01), and the scores of the primary symptoms and secondary symptoms of TCM, as well as the total score were lower than those of the control group (<i>P</i><0.001, <i>P</i><0.01). After treatment, the bilateral peak systolic velocity (PSV) and end diastolic velocity (EDV) were increased in the experimental group (<i>P</i><0.001), the right EDV was increased in the control group (<i>P</i><0.001), and the bilateral PSV and EDV in the experimental group were higher than those of the control group (<i>P<</i>0.05, <i>P</i><0.01, <i>P<</i>0.001). After treatment, there was no significant difference in the total area of carotid plaque between the two groups. After treatment, there were no significant abnormalities in blood routine and liver and kidney functions in both groups, and the safety was good.</p><p><strong>Conclusions: </strong>On the basis of conventional medication, the herbal cake-insulated moxibustion as an auxiliary treatment, presents its synergistic effect on CAS of interaction of phlegm and blood stasis syndrome in the patients, and this therapy is safe in clinical application.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 11","pages":"1325-1332"},"PeriodicalIF":0.0,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-25DOI: 10.13702/j.1000-0607.20241299
Li-Na Zhao, Xiao-Gang DU, Hu-Jie Song, Ning-Xia Zhao, Bo-Xia Li, Ting Li
Objectives: To assess the efficacy and safety of scalp acupuncture in the treatment of autism spectrum disorder (ASD).
Methods: A comprehensive search was conducted using PubMed, EMbase, The Cochrane Library, Web of Science, SinoMed, China National Knowledge Internet, China Science and Technology Journal Database, and Wanfang Data databases. The Cochrane Handbook of Systematic Reviews 5.1.0 was used to evaluate the risk of bias in the included randomized control trials (RCTs). A Meta-analysis was performed using RevMan 5.4.1 statistical software.
Results: A total of 33 RCTs were included, including 2 701 patients with ASD. Meta-analysis results showed that compared with the rehabilitation training group, the scalp acupuncture combined with rehabilitation training could significantly reduce the autism behavior rating scale score [MD= -5.70, 95% CI (-6.51, -4.89), P<0.01], and childhood autism rating scale score [MD= -4.02, 95% CI (-4.38, -3.66), P<0.01], and improve the Gesell developmental diagnostic scale-social adaptative developmental quotient [MD=5.90, 95% CI (4.29, 7.51), P<0.01], Gesell-language development quotient [MD=4.39, 95% CI (3.03, 5.75), P<0.01], Gesell-personal social competence [MD=4.32, 95% CI (2.48, 6.15), P<0.01], psychoeducational assessment for children 3rd edition (PEP-3)-communication skills score [MD=3.76, 95% CI (3.25, 4.27), P<0.01], PEP-3-physical agility score [MD=1.99, 95% CI (1.40, 2.59), P<0.01], and PEP-3-behavior score [MD=2.18, 95% CI (1.77, 2.59), P<0.01].
Conclusions: Scalp acupuncture is effective in improving the language problems, behavior problems, social adaptation and other symptoms of autism children, and has high safety. However, due to the insufficient quality of the research methods included in the literature, the integration of subjects with different severity and age, and the wide variation in the duration of the intervention, the conclusion of this study still needs to be validated by more rigorous and high-quality randomized controlled trials, with long-term follow up.
目的：评价头皮针刺治疗自闭症谱系障碍（ASD）的疗效和安全性。方法：综合检索PubMed、EMbase、Cochrane Library、Web of Science、SinoMed、中国知识互联网、中国科技期刊库和万方数据库。采用Cochrane系统评价手册5.1.0对纳入的随机对照试验（RCTs）进行偏倚风险评价。采用RevMan 5.4.1统计软件进行meta分析。结果：共纳入33项rct，共纳入2701例ASD患者。meta分析结果显示，与康复训练组相比，头皮针刺联合康复训练组可显著降低自闭症行为评定量表得分[MD= -5.70, 95% CI (-6.51, -4.89)， PMD= -4.02, 95% CI (-4.38, -3.66)， PMD=5.90, 95% CI (4.29, 7.51)， PMD=4.39, 95% CI (3.03, 5.75)， PMD=4.32, 95% CI (2.48, 6.15)]， Prd版(PEP-3)-沟通技能得分[MD=3.76, 95% CI (3.25, 4.27)， PMD=1.99, 95% CI (1.40, 2.59)， PMD=2.18， PMD=2.18]，结论：头皮针刺可有效改善自闭症儿童的语言问题、行为问题、社会适应等症状，且具有较高的安全性。然而，由于文献纳入的研究方法质量不足，且纳入的受试者严重程度和年龄不同，干预时间差异较大，本研究结论仍需通过更严格、高质量的随机对照试验、长期随访来验证。
{"title":"[Meta-analysis of efficacy and safety of scalp acupuncture in the treatment of autism spectrum disorder].","authors":"Li-Na Zhao, Xiao-Gang DU, Hu-Jie Song, Ning-Xia Zhao, Bo-Xia Li, Ting Li","doi":"10.13702/j.1000-0607.20241299","DOIUrl":"10.13702/j.1000-0607.20241299","url":null,"abstract":"<p><strong>Objectives: </strong>To assess the efficacy and safety of scalp acupuncture in the treatment of autism spectrum disorder (ASD).</p><p><strong>Methods: </strong>A comprehensive search was conducted using PubMed, EMbase, The Cochrane Library, Web of Science, SinoMed, China National Knowledge Internet, China Science and Technology Journal Database, and Wanfang Data databases. The Cochrane Handbook of Systematic Reviews 5.1.0 was used to evaluate the risk of bias in the included randomized control trials (RCTs). A Meta-analysis was performed using RevMan 5.4.1 statistical software.</p><p><strong>Results: </strong>A total of 33 RCTs were included, including 2 701 patients with ASD. Meta-analysis results showed that compared with the rehabilitation training group, the scalp acupuncture combined with rehabilitation training could significantly reduce the autism behavior rating scale score [<i>MD</i>= -5.70, 95% <i>CI</i> (-6.51, -4.89), <i>P</i><0.01], and childhood autism rating scale score [<i>MD</i>= -4.02, 95% <i>CI</i> (-4.38, -3.66), <i>P</i><0.01], and improve the Gesell developmental diagnostic scale-social adaptative developmental quotient [<i>MD</i>=5.90, 95% <i>CI</i> (4.29, 7.51), <i>P</i><0.01], Gesell-language development quotient [<i>MD</i>=4.39, 95% <i>CI</i> (3.03, 5.75), <i>P</i><0.01], Gesell-personal social competence [<i>MD</i>=4.32, 95% <i>CI</i> (2.48, 6.15), <i>P</i><0.01], psychoeducational assessment for children 3<sup>rd</sup> edition (PEP-3)-communication skills score [<i>MD</i>=3.76, 95% <i>CI</i> (3.25, 4.27), <i>P</i><0.01], PEP-3-physical agility score [<i>MD</i>=1.99, 95% <i>CI</i> (1.40, 2.59), <i>P</i><0.01], and PEP-3-behavior score [<i>MD</i>=2.18, 95% <i>CI</i> (1.77, 2.59), <i>P</i><0.01].</p><p><strong>Conclusions: </strong>Scalp acupuncture is effective in improving the language problems, behavior problems, social adaptation and other symptoms of autism children, and has high safety. However, due to the insufficient quality of the research methods included in the literature, the integration of subjects with different severity and age, and the wide variation in the duration of the intervention, the conclusion of this study still needs to be validated by more rigorous and high-quality randomized controlled trials, with long-term follow up.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 11","pages":"1352-1364"},"PeriodicalIF":0.0,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-25DOI: 10.13702/j.1000-0607.20241055
Xiao Mei, Wei Wang, Xiao-Fei Li, Hai-di Pan, Hua-Liang Deng
Objectives: To observe the clinical efficacy of acupoint application in the treatment of diarrhea type irritable bowel syndrome (IBS-D) with spleen deficiency and dampness retention.
Methods: 88 patients with spleen deficiency and dampness retention type IBS-D were randomly divided into a control group (44 cases, 1 case dropped out, 1 exclusion) and an experimental group (44 cases, 2 cases dropped out). The control group was given Bifidobacterium triple active bacteria capsules (Bifico) orally, 0.48 g each time, twice a day. Patients in the experimental group applied joint acupoint plaster to Shenque (CV8) in addition to the control group for 12 h each time, once a day. Both groups underwent treatment for 7 d per course, totaling 4 courses. The traditional Chinese medicine syndrome score, IBS severity scale (IBS-SSS) score, and IBS stool characteristics questionnaire (IBS-DSQ) score of the 2 groups were recorded before and after 4 weeks of treatment, and the clinical efficacy of the 2 groups were observed.
Results: After treatment, the TCM syndrome score, IBS-SSS score, and IBS-DSQ score of both groups of patients was decreased compared to those before treatment (P<0.001), and the experimental group was significantly better than the control group (P<0.01, P<0.001, P<0.05);the symptom scores of diarrhea, abdominal pain, and abdominal fullness in the experimental group were significantly lower than those in the control group (P<0.01, P<0.05), while there were no statistically significant difference in the scores of fatigue and drowsiness, and anorexia between the two groups. The total effective rate of the experimental group was 88.1% (37/42), which was higher than the control group's 73.8% (31/42), and the difference was statistically significant (P<0.05).
Conclusions: Acupoint application at CV8 can effectively improve diarrhea, abdominal pain, abdominal distension, and stool characteristics in patients with spleen deficiency and dampness retention type IBS-D, and improve their quality of life. The efficacy is definite, safe, and reliable, and it is worthy of further clinical promotion and application.
目的：观察穴位贴敷治疗腹泻型肠易激综合征（IBS-D）脾虚湿阻型的临床疗效。方法：88例脾虚湿阻型IBS-D患者随机分为对照组（44例，退出1例，排除1例）和实验组（44例，退出2例）。对照组患者给予双歧杆菌三联活菌胶囊（Bifico）口服，每次0.48 g，每日2次。实验组患者在对照组的基础上，在肾穴（CV8）处涂抹关节穴位膏药，每次12 h，每日1次。两组均治疗7 d /个疗程，共4个疗程。记录两组患者治疗前后4周的中医证候评分、IBS严重程度量表（IBS- sss）评分、IBS粪便特征问卷（IBS- dsq）评分，并观察两组患者的临床疗效。结果：治疗后，两组患者中医证候评分、IBS-SSS评分、IBS-DSQ评分均较治疗前下降（ppppppp）。结论：穴位贴敷CV8可有效改善脾虚湿阻型IBS-D患者腹泻、腹痛、腹胀、大便等特征，提高患者的生活质量。疗效明确、安全可靠，值得临床进一步推广应用。
{"title":"[Acupoint application therapy for diarrhea-predominant irritable bowel syndrome with spleen deficiency and dampness retention: a randomized controlled trial].","authors":"Xiao Mei, Wei Wang, Xiao-Fei Li, Hai-di Pan, Hua-Liang Deng","doi":"10.13702/j.1000-0607.20241055","DOIUrl":"10.13702/j.1000-0607.20241055","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the clinical efficacy of acupoint application in the treatment of diarrhea type irritable bowel syndrome (IBS-D) with spleen deficiency and dampness retention.</p><p><strong>Methods: </strong>88 patients with spleen deficiency and dampness retention type IBS-D were randomly divided into a control group (44 cases, 1 case dropped out, 1 exclusion) and an experimental group (44 cases, 2 cases dropped out). The control group was given Bifidobacterium triple active bacteria capsules (Bifico) orally, 0.48 g each time, twice a day. Patients in the experimental group applied joint acupoint plaster to Shenque (CV8) in addition to the control group for 12 h each time, once a day. Both groups underwent treatment for 7 d per course, totaling 4 courses. The traditional Chinese medicine syndrome score, IBS severity scale (IBS-SSS) score, and IBS stool characteristics questionnaire (IBS-DSQ) score of the 2 groups were recorded before and after 4 weeks of treatment, and the clinical efficacy of the 2 groups were observed.</p><p><strong>Results: </strong>After treatment, the TCM syndrome score, IBS-SSS score, and IBS-DSQ score of both groups of patients was decreased compared to those before treatment (<i>P</i><0.001), and the experimental group was significantly better than the control group (<i>P</i><0.01, <i>P</i><0.001, <i>P</i><0.05);the symptom scores of diarrhea, abdominal pain, and abdominal fullness in the experimental group were significantly lower than those in the control group (<i>P</i><0.01, <i>P</i><0.05), while there were no statistically significant difference in the scores of fatigue and drowsiness, and anorexia between the two groups. The total effective rate of the experimental group was 88.1% (37/42), which was higher than the control group's 73.8% (31/42), and the difference was statistically significant (<i>P</i><0.05).</p><p><strong>Conclusions: </strong>Acupoint application at CV8 can effectively improve diarrhea, abdominal pain, abdominal distension, and stool characteristics in patients with spleen deficiency and dampness retention type IBS-D, and improve their quality of life. The efficacy is definite, safe, and reliable, and it is worthy of further clinical promotion and application.</p>","PeriodicalId":34919,"journal":{"name":"针刺研究","volume":"50 11","pages":"1317-1324"},"PeriodicalIF":0.0,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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