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Engaging the community and building public trust in health research. 让社区参与并建立公众对卫生研究的信任。
Pub Date : 2023-06-16 DOI: 10.21428/3d48c34a.180cc7cb
Mosamat Umma Kulsum, Ishtiaq Hossain Ornab
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引用次数: 0
Impact of Sputum Transit Time on TB Isolation and Culture Contamination Rate 痰液转运时间对结核分离及培养污染率的影响
Pub Date : 2023-06-16 DOI: 10.21428/3d48c34a.c7202307
T. T. Eliya, Chukwuebuka Ugwu, Bimba John, Bassey Emmana, Kushim Jonathan, Ajide Bukola Adeoola, Ishaku Precious, Ekwu James
Tuberculosis (TB) culture is the gold standard for the diagnosis of Mycobacterium tuberculosis (Mtb) and enables full drug susceptibility testing. However, culture contamination remains a challenge in TB culture resulting to significant loss of time and resources and potentially, poor treatment outcomes on account of incorrect clinical decision-making. The purpose of this study is to assess the effect of sputum transit time on contamination rate and the yield of Mtb. We conducted a retrospective analysis of all samples received for TB culture at a Reference Laboratory, from January to December 2021. Samples were decontaminated by using the standard N-Acetyl L-cysteine (NALC)/Sodium Hydroxide (NaOH) method to process the sputum samples and inoculated on solid media. The number of days from sample collection to processing was documented for all 988 valid samples received within the study period. We conducted univariable logistic regression to explore the relationship between transit time and contamination rate. We report percentages, and Odds ratios with associated 95% confidence intervals. Of 988 diagnosis and follow-up samples received, 508(58.7%) were from males while 14(1.4%) were from children (≤ 14y). Overall, 56 samples were contaminated of which 17 were positive on AFB. A contamination rate of 4.1%, 7.1% and 8.3% were recorded for Optimal (0-7 days), Delayed (8-14 days), and Extended delay (>15 days) samples respectively. Compared to samples analyzed within optimal transit time, those with Delayed and Extended delay transit times had greater odds of being contaminated with OR of 1.811 (0.997-3.290) and 2.148 (0.983-4.688) respectively. The study revealed that TB culture contamination rate tends to increase with number of days taken for sample movement from collection to processing. However, the association detected fell slightly short of achieving statistical significance. It is also instructive that about one in three samples that were contaminated (17/56) was positive on AFB which could have far-reaching implications for the clinical decision-making and management of persons being
结核(TB)培养是诊断结核分枝杆菌(Mtb)的金标准,可以进行全面的药敏试验。然而,培养物污染仍然是结核病培养中的一个挑战,导致大量时间和资源的损失,并可能由于不正确的临床决策而导致治疗结果不佳。本研究的目的是评估痰液传递时间对结核分枝杆菌污染率和产率的影响。我们对2021年1月至12月在参考实验室收到的所有结核培养样本进行了回顾性分析。采用标准n -乙酰l -半胱氨酸(NALC)/氢氧化钠(NaOH)法对痰液进行净化处理,接种于固体培养基上。在研究期间收到的所有988份有效样本,从样本收集到处理的天数都被记录下来。我们进行了单变量逻辑回归来探讨运输时间和污染率之间的关系。我们报告百分比,比值比和相关的95%置信区间。在收到的988份诊断和随访样本中,男性508份(58.7%),儿童14份(1.4%)(≤14岁)。总共有56份样本受到污染,其中17份呈AFB阳性。最佳(0-7天)、延迟(8-14天)和延长延迟(>15天)样品的污染率分别为4.1%、7.1%和8.3%。与最佳转运时间内的样品相比,延迟转运时间和延长转运时间的样品被OR污染的几率分别为1.811(0.997-3.290)和2.148(0.983-4.688)。研究表明,结核培养物的污染率随着样品从收集到处理所需的天数的增加而增加。然而,检测到的关联略低于达到统计显著性。同样具有指导意义的是,大约三分之一的污染样本(17/56)对AFB呈阳性,这可能对患者的临床决策和管理产生深远的影响
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引用次数: 0
Implementation of Data Platforms in the context of Open Science: the experience of Fiocruz 开放科学背景下数据平台的实现:Fiocruz的经验
Pub Date : 2023-06-16 DOI: 10.21428/3d48c34a.de00f2c6
V. Jorge, C. Machado
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引用次数: 0
Towards Achieving improved access to youth-friendly Sexual Reproductive Health and Rights (SRHR) services in Cross River State (CRS) Nigeria; Sustaining Evidence-Driven Advocacy through the CRS SRHR policy. 争取在尼日利亚克罗斯河州改善获得对青年友好的性生殖健康和权利服务的机会;通过CRS SRHR政策维持循证倡导。
Pub Date : 2023-06-16 DOI: 10.21428/3d48c34a.66ca725e
G. Odey, Madonna Badom, H. Obaje
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引用次数: 0
Community Health Volunteers and their role in facilitating adoption of health interventions in the community. 社区卫生志愿者及其在促进社区采用卫生干预措施方面的作用。
Pub Date : 2023-06-16 DOI: 10.21428/3d48c34a.8a98998e
Charity Hungu, Relebogile Mapuroma, J. Ncayiyana, M. Atela
They
他们
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引用次数: 0
Diabetes mellitus as a cause of disability in Mexico from 1998 to 2020. 从1998年到2020年,墨西哥的糖尿病是导致残疾的一个原因。
Pub Date : 2023-06-16 DOI: 10.21428/3d48c34a.119d9c4e
Yaocihuatl Castañeda Borrayo, S. L. Cortés, Leslie Guadalupe Rivera Rodríguez
Yaocihuatl Castañeda Borrayo1 Silvia Graciela León Cortés2 Leslie Guadalupe Rivera Rodríguez2 1Instituto Mexicano del Seguro Social, Unidad de Investigación Epidemiológica y en Servicios de Salud, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social (IMSS), Órganos de Operación Administrativa Desconcentrada., Mexico, 2University of Guadalajara, University Center for Health Sciences, Department of Public Health, Doctorate in Occupational Health Sciences., Mexico
Yaocihuatl castaneda Borrayo1 Silvia Graciela leon corte2 Leslie Guadalupe Rivera rodriguez2 1墨西哥社会保障研究所,流行病学和卫生服务研究单位,国家西方医学中心,墨西哥社会保障研究所(IMSS),行政权力下放机构。墨西哥,瓜达拉哈拉大学,大学卫生科学中心,公共卫生部,职业卫生科学博士。墨西哥
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引用次数: 0
Trends of children and adolescents in HIV care during and after the COVID19 lock-down in Uganda, 2020 – 2021: a descriptive analysis of surveillance data 2020 - 2021年乌干达covid - 19封锁期间和之后儿童和青少年接受艾滋病毒护理的趋势:监测数据的描述性分析
Pub Date : 2023-06-16 DOI: 10.21428/3d48c34a.ab93d1a0
Miriam Nakanwagi, Dennis Adoa, Esther Nyamugisa, E. Magongo
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引用次数: 0
Evaluation of Schistosoma haematobium and Access to Wash Facilities by School Age Children in Osogbo Metropolis, Osun State, Nigeria. 尼日利亚奥孙州奥索博市学龄儿童血血吸虫病和获得洗涤设施的评估
Pub Date : 2023-06-16 DOI: 10.21428/3d48c34a.52e86d6d
Abolanle Adebisi, M. Adeleke, K. Fasasi, O. Surakat
Background: Schistosomiasis is the most prevalent waterborne parasitic diseases in rural communities and urban populations which depend on streams and rivers for domestic and agricultural purposes. Provision of water, sanitation and hygiene (WASH) resources has been advocated a strategy for sustainable control of soil-transmitted helminthiasis (STH). Studies on Schistosoma haematobium amongst school children in osogbo metropolis in Osun state, Nigeria was carried out to determine the prevalence of schistosomiasis in school age children and the status of WASH resources. Method: A total of 216 (104 males and 112 females) urine was collected in a collection tube. Schistosoma haematobium infection was detected by microscopic examination of schistosome egg in the urine. A structured survey form (Questionnaire) was administered to each participants to inquiry about their demographic characteristics, sanitation and hygiene practices (WASH activity) both at home and in the school and also their knowledge about schistosomiasis using WHO standard questionnaire. The samples was centrifuged and kept in the fridge at 40C until further analysis. 50 samples were further analyzed for DNA extraction using the Genomic DNA Extraction kits by NIMR BIOTECH (Research for national Health), extracted/ eluted DNA was stored at -20oC in the freezer. The supposed DNAs obtained were subjected to Polymerase Chain Reaction (PCR) using the conventional method to detect the scistosome DNA. Result: But none of the 50 samples pool tested was positive to any of the target DNA gel electrophorensis. This study observed no detectable DNA in the urine samples. Conclusion: It is hence suggested that there was little risk of prevalence of schistosomiasis infection in Osogbo during this study period. However, further investigations, as well as, continual prevalence investigation are recommended.
背景:血吸虫病是农村社区和城市人口中最普遍的水传播寄生虫病,这些社区和城市人口的生活和农业目的依赖于溪流和河流。提供水、环境卫生和个人卫生(WASH)资源是一项可持续控制土壤传播蠕虫病(STH)的战略。对尼日利亚奥松州奥索博市学龄儿童中的血血吸虫病进行了研究,以确定学龄儿童中血吸虫病的流行情况和讲卫生资源的状况。方法:采用尿管收集216例(男104例,女112例)尿液。尿中血吸虫卵镜检发现血血吸虫感染。向每位参与者发放了一份结构化调查表(问卷),以使用世卫组织标准问卷询问他们的人口特征、家庭和学校的环境卫生和个人卫生习惯(WASH活动)以及他们对血吸虫病的了解情况。样品被离心并保存在40摄氏度的冰箱里,等待进一步的分析。使用NIMR BIOTECH (Research for national Health)的基因组DNA提取试剂盒对50份样本进行进一步分析提取DNA,提取/洗脱的DNA在-20℃冷冻保存。将得到的假定DNA进行聚合酶链反应(PCR),采用常规方法检测裂体DNA。结果:50份样本均无靶DNA凝胶电泳阳性。本研究未在尿液样本中发现可检测到的DNA。结论:本研究期间,奥索博省血吸虫病流行风险较小。但是,建议进行进一步的调查以及持续的流行率调查。
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引用次数: 0
A simple high-performance liquid chromatographic assay for lumefantrine in plasma in the presence of efavirenz-based antiretroviral therapy 在以依非韦伦为基础的抗逆转录病毒治疗下,血浆中氨苯曲明的简单高效液相色谱分析
Pub Date : 2023-06-16 DOI: 10.21428/3d48c34a.d27f28ed
Ruth M. Ogboye, J. Soyinka, O. Bolaji, A. Adegbola
As per current treatment guidelines, artemether-lumefantrine, and efavirenz (EFV)-based antiretroviral therapy are recommended drugs for falciparum malaria and HIV infections, respectively. A liquid chromatography-ultraviolet detection (LC-UV) method for simultaneous quantification of lumefantrine and EFV was developed and validated for efficacy and pharmacokinetic clinical studies. Lumefantrine and EFV were separated using a 100 x 4.6 mm x 3 µm Fortis C 18 chromatographic column, and a multistep gradient mobile phase. Calibration was obtained with a series of standard solutions containing known concentrations of the chemical reference of both analytes prepared concomitantly in drug-free plasma. The assay was validated within the calibration ranges of 78.125 to 20000 ng/mL for lumefantrine and 187.15 to 24000 ng/mL for EFV. Stability assessment was carried out with or without heating the quality sample to 58°C for 45 min. The method was employed to measure the plasma concentrations of lumefantrine and EFV in a study conducted among malaria-HIV co-infected patients. Lumefantrine and EFV were well separated from each other and from the biological matrix. The method demonstrated a good recovery of 72.64% for lumefantrine and 117.17% for EFV. The intra-and inter-day accuracy presented as 95.36-105.14% for lumefantrine and 104.11-115% for EFV and precision ranged from 1.15 to 6.45% for lumefantrine and 0.43 to 13.12 for EFV, were within ±15% at the three quality control levels. The analytes from both quality control lots and samples collected from HIV-malaria co-infected individuals were found to be stable post-deactivation of infectious virus by heating to 58 °C for 45 min. The assay is accurate, precise and shown to simultaneously measure the lumefantrine and EFV in human
根据目前的治疗指南,推荐分别使用以蒿甲醚-氨苯曲明和依非韦伦(EFV)为基础的抗逆转录病毒疗法治疗恶性疟疾和艾滋病毒感染。采用液相色谱-紫外检测(LC-UV)方法同时定量研究了氟苯曲明和EFV的药效和药动学。采用100 × 4.6 mm × 3µm Fortis C 18色谱柱和多级梯度流动相分离发光方汀和EFV。校准是用一系列标准溶液获得的,这些标准溶液含有在无药血浆中同时制备的两种分析物的化学参考物的已知浓度。该方法在校准范围内进行了验证,荧光曲明的校准范围为78.125 ~ 20000 ng/mL, EFV的校准范围为187.15 ~ 24000 ng/mL。稳定性评估分别在加热或不加热质量样品至58°C 45分钟的情况下进行。该方法用于在疟疾- hiv合并感染患者中测量lumantrine和EFV的血浆浓度。发光芳碱与EFV相互分离良好,并与生物基质分离良好。该法回收率为72.64%,EFV为117.17%。在三个质量控制水平下,日间和日间荧光素的准确度分别为95.36 ~ 105.14%和104.11 ~ 115%,荧光素的准确度分别为1.15 ~ 6.45%和0.43 ~ 13.12,均在±15%以内。从质量控制批次和hiv -疟疾合并感染个体收集的样品中发现,在感染性病毒灭活后,加热至58°C 45分钟,分析物是稳定的。该测定方法准确、精确,可以同时测量人体内的甲苯胺和EFV
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引用次数: 0
Knowledge of Tuberculosis Disease Among Newly Diagnosed Patients: A cross Sectional study 新诊断患者对结核病的认识:一项横断面研究
Pub Date : 2023-06-16 DOI: 10.21428/3d48c34a.6e26a90e
E. L. Acen, I. A. Biraro, M. Bbuye, D. Kateete, M. Joloba, W. Worodria
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引用次数: 0
期刊
The Global Health Network Conference Proceedings 2022
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