of a thesis for a Doctorate of Philosophy submitted to Macquarie University, Sydney Mo Neurone Disease (MND) is characterised by the loss of motor neurons in the brain and spinal cord. Most patients with MND develop proteinaceous inclusions within affected neurons of the central nervous system, suggesting overall] dysregulation of protein degradation systems. Our team identified mutations in CCNF, the gene encoding cyclin F. Cyclin F is a substrate binding component of a multi-protein ubiquitin ligase (denoted SCFo«linF) | which mediates the ubiquitylation of substrates in order to influence the cell cycle. Overarching aims of this thesis concern the impact of CCNF mutations on the ubiquitin ligase activity of SCFeelin F and the downstream impact within cells. Addressing these aims involved using a series of biochemical assays (including in VItTO ubiquitylation assays, immunoprecipi-tations, proximity-ligation assays and mass spectrometry). Results demonstrate that an MND-linked mutation in cyclin F leads to defective ubiquitylation activity, ultimately leading to the accumulation of proteins tagged for degradation. Overall, the work provides insight into how the precise control of cyclin F ligase activity is dysregulated when cyclin F carries a disease-causing mutation. Furthermore, outcomes from this work provide novel links between cyclin F (a cell cycle regulator) and a devastating disease involving the degeneration of post-mitotic neurons.
{"title":"The role of cyclin F in Motor Neurone Disease","authors":"Stephanie L. Rayner","doi":"10.5962/p.361941","DOIUrl":"https://doi.org/10.5962/p.361941","url":null,"abstract":"of a thesis for a Doctorate of Philosophy submitted to Macquarie University, Sydney Mo Neurone Disease (MND) is characterised by the loss of motor neurons in the brain and spinal cord. Most patients with MND develop proteinaceous inclusions within affected neurons of the central nervous system, suggesting overall] dysregulation of protein degradation systems. Our team identified mutations in CCNF, the gene encoding cyclin F. Cyclin F is a substrate binding component of a multi-protein ubiquitin ligase (denoted SCFo«linF) | which mediates the ubiquitylation of substrates in order to influence the cell cycle. Overarching aims of this thesis concern the impact of CCNF mutations on the ubiquitin ligase activity of SCFeelin F and the downstream impact within cells. Addressing these aims involved using a series of biochemical assays (including in VItTO ubiquitylation assays, immunoprecipi-tations, proximity-ligation assays and mass spectrometry). Results demonstrate that an MND-linked mutation in cyclin F leads to defective ubiquitylation activity, ultimately leading to the accumulation of proteins tagged for degradation. Overall, the work provides insight into how the precise control of cyclin F ligase activity is dysregulated when cyclin F carries a disease-causing mutation. Furthermore, outcomes from this work provide novel links between cyclin F (a cell cycle regulator) and a devastating disease involving the degeneration of post-mitotic neurons.","PeriodicalId":35531,"journal":{"name":"Journal and Proceedings - Royal Society of New South Wales","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45328313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
....................................................................................................................... xv Structure of the thesis ................................................................................................ xix List of definitions .......................................................................................................... xxi Chapter 1: Introduction .............................................................................................. 1 1.1 Background and significance ......................................................................... 1 1.2 Study aim ............................................................................................................. 6 1.3 Research questions ............................................................................................ 6 1.4 Chapter conclusion ........................................................................................... 6 Chapter 2: Literature review ....................................................................................... 7 2.
{"title":"Investigating undergraduate nurse responses to simulated interruptions during medication administration--a qualitative multi-method study","authors":"C. Hayes","doi":"10.5962/p.361932","DOIUrl":"https://doi.org/10.5962/p.361932","url":null,"abstract":"....................................................................................................................... xv Structure of the thesis ................................................................................................ xix List of definitions .......................................................................................................... xxi Chapter 1: Introduction .............................................................................................. 1 1.1 Background and significance ......................................................................... 1 1.2 Study aim ............................................................................................................. 6 1.3 Research questions ............................................................................................ 6 1.4 Chapter conclusion ........................................................................................... 6 Chapter 2: Literature review ....................................................................................... 7 2.","PeriodicalId":35531,"journal":{"name":"Journal and Proceedings - Royal Society of New South Wales","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49370588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Chief Scientist is right, and why","authors":"P. Rez","doi":"10.5962/p.361925","DOIUrl":"https://doi.org/10.5962/p.361925","url":null,"abstract":"","PeriodicalId":35531,"journal":{"name":"Journal and Proceedings - Royal Society of New South Wales","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42741536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Far from having only marginal significance and generating a ‘subdued’ response among African Americans, as some historians have argued, the Nigerian Civil War (1967-1970) collided at full velocity with the conflicting discourses and ideas by which black Americans sought to understand their place in the United States and the world in the late 1960s. Black liberal civil rights leaders leapt to offer their service as agents of direct diplomacy during the conflict, seeking to preserve Nigerian unity; grassroots activists from New York to Kansas organised food-drives, concerts and awareness campaigns in support of humanitarian aid for Biafran victims of starvation; while other pro-Biafran black activists warned of links between black ‘genocide’ in Biafra and the US alike. This thesis is the first to recover and analyse at length the extent, complexity and character of such African American responses to the Nigerian Civil War. Drawing on extensive use of private papers, activist literature, government records and especially the black press, it charts the way African Americans conceptualised, over time and in complex ways, their varied understandings of issues such as black internationalist solidarities, territorial sovereignty and political viability, humanitarian compassion and great power realpolitik, as well as colonial and neo-colonial influence in Africa. The thesis initially explores the longer twentieth century history of African American engagement with Nigeria by way of establishing context, before providing in-depth analysis of the key initiatives and events that comprised African American engagement with the civil war. Chapters move chronologically and thematically to discuss direct diplomatic efforts to broker peace, African American responses to alleged genocide in Biafra, the rise and fall of pro-Biafran political support, and the latter’s loss to what emerged as a stronger political bloc of those supporting Nigerian political unity. Situated methodologically and historiographically at the intersection of scholarship on black internationalism and the international history of the Nigerian Civil War, this thesis demonstrates the way the civil war not only provoked intense activism, but did so in ways that fundamentally connected with the central ideas, themes and concerns of the black freedom struggle in the United States.
{"title":"Black America Cares: The response of African Americans to the Nigerian Civil War, 1967-1970","authors":"J. Farquharson","doi":"10.5962/p.361930","DOIUrl":"https://doi.org/10.5962/p.361930","url":null,"abstract":"Far from having only marginal significance and generating a ‘subdued’ response among African Americans, as some historians have argued, the Nigerian Civil War (1967-1970) collided at full velocity with the conflicting discourses and ideas by which black Americans sought to understand their place in the United States and the world in the late 1960s. Black liberal civil rights leaders leapt to offer their service as agents of direct diplomacy during the conflict, seeking to preserve Nigerian unity; grassroots activists from New York to Kansas organised food-drives, concerts and awareness campaigns in support of humanitarian aid for Biafran victims of starvation; while other pro-Biafran black activists warned of links between black ‘genocide’ in Biafra and the US alike. This thesis is the first to recover and analyse at length the extent, complexity and character of such African American responses to the Nigerian Civil War. Drawing on extensive use of private papers, activist literature, government records and especially the black press, it charts the way African Americans conceptualised, over time and in complex ways, their varied understandings of issues such as black internationalist solidarities, territorial sovereignty and political viability, humanitarian compassion and great power realpolitik, as well as colonial and neo-colonial influence in Africa. The thesis initially explores the longer twentieth century history of African American engagement with Nigeria by way of establishing context, before providing in-depth analysis of the key initiatives and events that comprised African American engagement with the civil war. Chapters move chronologically and thematically to discuss direct diplomatic efforts to broker peace, African American responses to alleged genocide in Biafra, the rise and fall of pro-Biafran political support, and the latter’s loss to what emerged as a stronger political bloc of those supporting Nigerian political unity. Situated methodologically and historiographically at the intersection of scholarship on black internationalism and the international history of the Nigerian Civil War, this thesis demonstrates the way the civil war not only provoked intense activism, but did so in ways that fundamentally connected with the central ideas, themes and concerns of the black freedom struggle in the United States.","PeriodicalId":35531,"journal":{"name":"Journal and Proceedings - Royal Society of New South Wales","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47624581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Belief change without compactness","authors":"Jandson Santo Ribeiro Santo","doi":"10.5962/p.361942","DOIUrl":"https://doi.org/10.5962/p.361942","url":null,"abstract":"","PeriodicalId":35531,"journal":{"name":"Journal and Proceedings - Royal Society of New South Wales","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48898810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Richard Limon Stanton DistFRSN (1926-2020)","authors":"R. Marks","doi":"10.5962/p.361946","DOIUrl":"https://doi.org/10.5962/p.361946","url":null,"abstract":"","PeriodicalId":35531,"journal":{"name":"Journal and Proceedings - Royal Society of New South Wales","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46558503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The impact of anthropogenic stressors on coral reef carbonate sediment metabolism and dissolution","authors":"C. Lantz","doi":"10.5962/p.361938","DOIUrl":"https://doi.org/10.5962/p.361938","url":null,"abstract":"","PeriodicalId":35531,"journal":{"name":"Journal and Proceedings - Royal Society of New South Wales","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46473048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Steffe, Jamin P. Forbes, R. Watts, L. Baumgartner, P. Brown, J. Thiem, N. Miles
Freshwater reservoirs support valuable commercial and recreational fisheries resources worldwide. Recreational fisheries in reservoirs are commonly managed using length-based harvest regulations, although empirical data is required to ensure these regulations are fit-for-purpose and that any changes do not result in negative population responses. Following a change from a 600 mm minimum legal length to a 550-750 mm harvest slot (HS) limit for both reservoir and river based recreational fisheries for Murray cod, Maccullochella peelii, we used a stratified random sampling design to assess the summer recreational fishery in Lake Mulwala, an important reservoir fishery. Specifically, we assessed the immediate effects of the HS on angler effort, catch and harvest in this fishery. Standardised parameters based on data from existing angler surveys were also used to investigate variability among fisheries for Murray cod. We found that (1) in Lake Mulwala, more than 50% of Murray cod harvested after the introduction of the HS were between 550 and 600 mm; (2) boat-based anglers almost exclusively targeted Murray cod and harvested more and larger fish than shore-based anglers; (3) the Murray cod population was severely truncated with high mandatory discard rates (93.7% for boat fisheries and 99.8% for shore fisheries) of fish below the lower HS limit; (4) standardised parameters varied among waterbodies, and comparatively higher effort, discard and harvest densities were observed in the reservoir fishery. The reliance on newly harvestable Murray cod in Lake Mulwala highlights the need for early and ongoing monitoring of regulatory changes across riverine and reservoir environments, encompassing both social and biological aspects of the fishery
{"title":"Implementation of a harvest slot for Murray Cod: Initial impacts on the recreational harvest in a manmade reservoir and comparison to riverine fisheries","authors":"A. Steffe, Jamin P. Forbes, R. Watts, L. Baumgartner, P. Brown, J. Thiem, N. Miles","doi":"10.5962/p.361916","DOIUrl":"https://doi.org/10.5962/p.361916","url":null,"abstract":"Freshwater reservoirs support valuable commercial and recreational fisheries resources worldwide. Recreational fisheries in reservoirs are commonly managed using length-based harvest regulations, although empirical data is required to ensure these regulations are fit-for-purpose and that any changes do not result in negative population responses. Following a change from a 600 mm minimum legal length to a 550-750 mm harvest slot (HS) limit for both reservoir and river based recreational fisheries for Murray cod, Maccullochella peelii, we used a stratified random sampling design to assess the summer recreational fishery in Lake Mulwala, an important reservoir fishery. Specifically, we assessed the immediate effects of the HS on angler effort, catch and harvest in this fishery. Standardised parameters based on data from existing angler surveys were also used to investigate variability among fisheries for Murray cod. We found that (1) in Lake Mulwala, more than 50% of Murray cod harvested after the introduction of the HS were between 550 and 600 mm; (2) boat-based anglers almost exclusively targeted Murray cod and harvested more and larger fish than shore-based anglers; (3) the Murray cod population was severely truncated with high mandatory discard rates (93.7% for boat fisheries and 99.8% for shore fisheries) of fish below the lower HS limit; (4) standardised parameters varied among waterbodies, and comparatively higher effort, discard and harvest densities were observed in the reservoir fishery. The reliance on newly harvestable Murray cod in Lake Mulwala highlights the need for early and ongoing monitoring of regulatory changes across riverine and reservoir environments, encompassing both social and biological aspects of the fishery","PeriodicalId":35531,"journal":{"name":"Journal and Proceedings - Royal Society of New South Wales","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46449682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Institutional influences on education investment and pro-social behaviour","authors":"Jing Chen","doi":"10.5962/p.361929","DOIUrl":"https://doi.org/10.5962/p.361929","url":null,"abstract":"","PeriodicalId":35531,"journal":{"name":"Journal and Proceedings - Royal Society of New South Wales","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48704363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Response to the 25 scientists","authors":"A. Finkel","doi":"10.5962/p.361921","DOIUrl":"https://doi.org/10.5962/p.361921","url":null,"abstract":"","PeriodicalId":35531,"journal":{"name":"Journal and Proceedings - Royal Society of New South Wales","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47742440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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