A. Rustaiyan, H. Nahrevania, Z. Zamani, M. Taherkhani, A. Iravani
Tehranolide which has been isolated from Artemisia diffusa has similar functional group to Artemisinin. More attention has been paid to Artemisia annua L., for its anti-plasmodial properties. In the present study, we investigated the anti-malarial effects of Tehranolide against human malaria parasite, Plasmodium falciparum in vitro. The chloroquine (CQ)-sensitive strain (3D7) of P. falciparum was continuously cultured in PRMI medium with addition of HT serum Albumax, RBC of O blood group, 05% CO2 at 37°C. The anti-malarial activity was determined by using different concentrations including 10, 30, 50 mg mLG of Tehranolide were made in drug vehicle including distilled water, methanol, DMSO and applied for therapy. Percentage of parasitaemia was counted after 24, 48 and 72 h after treatment for each concentration. Results indicated no effects of low concentration of Tehranolide on parasitaemia, however the concentrations of 10, 30 and 50 mg mLG represented their anti-plasmodial activities. The cytotoxic effects of high concentration occurred by destroying both parasites and RBCs in culture medium. Inhibition concentration of 50% (IC50) on plasmodial survival was observed at concentration of 10 mg mLG after 48-72 h of treatment. It is concluded that, Tehranolide seems to be a promising drug exhibiting good anti-malarial effects in this human malaria P. falciparum model in vitro. However, more research is required before Tehranolide can be used for malaria treatment in human cases.
{"title":"An Investigation on Anti-malarial Effects of Tehranolide Isolated from Artemisia diffusa Against Human Malaria Parasite, Plasmodium falciparum in vitro","authors":"A. Rustaiyan, H. Nahrevania, Z. Zamani, M. Taherkhani, A. Iravani","doi":"10.3923/JP.2015.73.78","DOIUrl":"https://doi.org/10.3923/JP.2015.73.78","url":null,"abstract":"Tehranolide which has been isolated from Artemisia diffusa has similar functional group to Artemisinin. More attention has been paid to Artemisia annua L., for its anti-plasmodial properties. In the present study, we investigated the anti-malarial effects of Tehranolide against human malaria parasite, Plasmodium falciparum in vitro. The chloroquine (CQ)-sensitive strain (3D7) of P. falciparum was continuously cultured in PRMI medium with addition of HT serum Albumax, RBC of O blood group, 05% CO2 at 37°C. The anti-malarial activity was determined by using different concentrations including 10, 30, 50 mg mLG of Tehranolide were made in drug vehicle including distilled water, methanol, DMSO and applied for therapy. Percentage of parasitaemia was counted after 24, 48 and 72 h after treatment for each concentration. Results indicated no effects of low concentration of Tehranolide on parasitaemia, however the concentrations of 10, 30 and 50 mg mLG represented their anti-plasmodial activities. The cytotoxic effects of high concentration occurred by destroying both parasites and RBCs in culture medium. Inhibition concentration of 50% (IC50) on plasmodial survival was observed at concentration of 10 mg mLG after 48-72 h of treatment. It is concluded that, Tehranolide seems to be a promising drug exhibiting good anti-malarial effects in this human malaria P. falciparum model in vitro. However, more research is required before Tehranolide can be used for malaria treatment in human cases.","PeriodicalId":364497,"journal":{"name":"Research Journal of Parasitology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128763002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Adie, A. Oyo-Ita, O. Okon, G. Arong, I. Atting, E. Braide, O. Nebe, U. Emanghe, A. Otu
A parasitological mapping of urinary schistosomiasis using filtration method was conducted in Biase and Yakurr LGAs of Cross River State, Nigeria by the Neglected Tropical Diseases Control unit in collaboration with the schistosomiasis/soil transmitted helminths unit of the Federal Ministry of Health, Nigeria in November 2012. The results of the study revealed a mean urinary schistosomiasis prevalence of 49% for the six schools under study in Biase and 30% for the six schools under study in Yakurr LGA. The mean ova load was 0.9 for males and 0.8 for females in the two LGAs. Integrated control measures put in place, included chemotherapy of infected individuals with praziquantel and health education on the predisposing factors responsible for the transmission of urinary schistosomiasis. An evaluation of the interventions was carried out in November 2014, after two rounds of treatment with Praziquantel and intensive education were given. Urine samples were collected from 600 school children, 300 from each of the two LGAs. The evaluation study using the urine filtration technique revealed a mean schistosomiasis prevalence of 0% for the six schools under study in Biase and 0.02% for the six schools under study in Yakurr LGA with mean ova load reduced to 0.3 for males and 0 for females in the two LGAs. Data analysis with SPSS package revealed a 100% participation of all selected school children in 12 schools (6 in each LGA). Statistical analysis showed that there was no significant difference in the prevalence between male and female (p>0.005). A student t-test showed a significant difference between prevalence rates in 2012 and the results in 2014 (p>0.005). The spatial distribution showed that endemic schools were distributed within marshy areas where rice was cultivated in the two LGAs. These results showed that with wide scale integrated control measures, urinary schistosomiasis can be eliminated or reduced to a disease of no public health importance.
{"title":"Evaluation of Intensity of Urinary Schistosomiasis in Biase and Yakurr Local Government Areas of Cross River State, Nigeria after Two Years of Integrated Control Measures","authors":"H. Adie, A. Oyo-Ita, O. Okon, G. Arong, I. Atting, E. Braide, O. Nebe, U. Emanghe, A. Otu","doi":"10.3923/JP.2015.58.65","DOIUrl":"https://doi.org/10.3923/JP.2015.58.65","url":null,"abstract":"A parasitological mapping of urinary schistosomiasis using filtration method was conducted in Biase and Yakurr LGAs of Cross River State, Nigeria by the Neglected Tropical Diseases Control unit in collaboration with the schistosomiasis/soil transmitted helminths unit of the Federal Ministry of Health, Nigeria in November 2012. The results of the study revealed a mean urinary schistosomiasis prevalence of 49% for the six schools under study in Biase and 30% for the six schools under study in Yakurr LGA. The mean ova load was 0.9 for males and 0.8 for females in the two LGAs. Integrated control measures put in place, included chemotherapy of infected individuals with praziquantel and health education on the predisposing factors responsible for the transmission of urinary schistosomiasis. An evaluation of the interventions was carried out in November 2014, after two rounds of treatment with Praziquantel and intensive education were given. Urine samples were collected from 600 school children, 300 from each of the two LGAs. The evaluation study using the urine filtration technique revealed a mean schistosomiasis prevalence of 0% for the six schools under study in Biase and 0.02% for the six schools under study in Yakurr LGA with mean ova load reduced to 0.3 for males and 0 for females in the two LGAs. Data analysis with SPSS package revealed a 100% participation of all selected school children in 12 schools (6 in each LGA). Statistical analysis showed that there was no significant difference in the prevalence between male and female (p>0.005). A student t-test showed a significant difference between prevalence rates in 2012 and the results in 2014 (p>0.005). The spatial distribution showed that endemic schools were distributed within marshy areas where rice was cultivated in the two LGAs. These results showed that with wide scale integrated control measures, urinary schistosomiasis can be eliminated or reduced to a disease of no public health importance.","PeriodicalId":364497,"journal":{"name":"Research Journal of Parasitology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131463767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correlations Among the Level of Homocysteine, Antioxidant Enzymes, Antioxidant Vitamins and Lipid Peroxidation of Erythrocytes in Malignant Ovine Theileriosis","authors":"S. Razavi, S. Nazifi, N. Mokhtari, E. Rakhshande","doi":"10.3923/JP.2015.42.49","DOIUrl":"https://doi.org/10.3923/JP.2015.42.49","url":null,"abstract":"","PeriodicalId":364497,"journal":{"name":"Research Journal of Parasitology","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130767082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Role of COX-2 in Pathogenesis of Intestinal Cryptosporidiosis and Effect of some Drugs on Treatment of Infection","authors":"G. Sadek, Bahaa El Deen W. El Aswa","doi":"10.3923/JP.2014.21.40","DOIUrl":"https://doi.org/10.3923/JP.2014.21.40","url":null,"abstract":"","PeriodicalId":364497,"journal":{"name":"Research Journal of Parasitology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121596093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. M. Sanad, F. Thagfan, E. Olayan, .. A.Almogren, .. A.AlHammaad, .. A.Al-Mawash, A. A. Mohamed
{"title":"Opportunistic Coccidian Parasites among Saudi Cancer Patients Presenting with Diarrhea: Prevalence and Immune Status","authors":"M. M. Sanad, F. Thagfan, E. Olayan, .. A.Almogren, .. A.AlHammaad, .. A.Al-Mawash, A. A. Mohamed","doi":"10.3923/JP.2014.55.63","DOIUrl":"https://doi.org/10.3923/JP.2014.55.63","url":null,"abstract":"","PeriodicalId":364497,"journal":{"name":"Research Journal of Parasitology","volume":"171 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116917616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amany A. Rady, Bahaa El Deen W. El Aswa, B. M. Masoud
{"title":"Comparative Evaluation of Different Diagnostic Techniques using Laminated Layer Antigen for Serodiagnosis of Human Hydatidosis","authors":"Amany A. Rady, Bahaa El Deen W. El Aswa, B. M. Masoud","doi":"10.3923/JP.2014.41.54","DOIUrl":"https://doi.org/10.3923/JP.2014.41.54","url":null,"abstract":"","PeriodicalId":364497,"journal":{"name":"Research Journal of Parasitology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126654094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Efficacy of Combined Therapy of Artemether and Somatostatin in Heptic Hydroxyproline in Experimental Murine Schistosomiasis mansoni","authors":"M. A. Hegazi, M. Mahmoud","doi":"10.3923/JP.2013.55.63","DOIUrl":"https://doi.org/10.3923/JP.2013.55.63","url":null,"abstract":"","PeriodicalId":364497,"journal":{"name":"Research Journal of Parasitology","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123848116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrastructure and Populational Ratios of Hemocytes of the Brown Dog Tick, Rhipicephalus sanguineus (Latreille) (Ixodoidea, Ixodidae)","authors":"A. Bakr, H. Gaber, H. MadihaM.Abdel, H. Senbill","doi":"10.3923/JP.2013.64.70","DOIUrl":"https://doi.org/10.3923/JP.2013.64.70","url":null,"abstract":"","PeriodicalId":364497,"journal":{"name":"Research Journal of Parasitology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127877275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Abakar, N. Khogali, A. Bashar, H. Seri, A. Tigani, A. A. Abdalmalai
{"title":"Prevalence of Strongylus vulgaris in the Mesenteric Artery of Donkey from Nyala, South Darfur State, Sudan","authors":"A. Abakar, N. Khogali, A. Bashar, H. Seri, A. Tigani, A. A. Abdalmalai","doi":"10.3923/JP.2013.71.75","DOIUrl":"https://doi.org/10.3923/JP.2013.71.75","url":null,"abstract":"","PeriodicalId":364497,"journal":{"name":"Research Journal of Parasitology","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131140573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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