I. Giubilei, F. Brugneti, S. Turco, M. I. Drais, A. Mazzaglia
European beech (Fagus sylvatica) is one of the most common deciduous forest species in Italy and across Europe, important for its economic, cultural and environmental value (Siddique et al., 2021). In June 2023, leaves showing anthracnose symptoms (Figure 1) were collected from the UNESCO site “Faggeta Vetusta di Monte Raschio” located in Oriolo Romano, Viterbo in central Italy. To identify the causal agent, leaves were surface sterilised by dipping successively in 70% ethanol for two minutes, 1% sodium hypochlorite for three minutes and 70% ethanol for one minute, and finally rinsed three times with sterile water (SDW). Slices obtained with a sterile scalpel were plated on potato dextrose agar plates and incubated at 25°C for 8 to 15 days. Developing fungal colonies had white to grey cottony mycelium and were pale orange with dark flecking from the reverse side (Figure 2). Conidia were hyaline, smooth-walled, aseptate, narrowly elliptical pointed at both ends, measuring 10.5-21 μm (mean 16.4 μm) × 3.5-7 μm (mean 4.3 μm) (Figure 3). The morphological characteristics fit published descriptions of Colletotrichum fioriniae (Shivas & Yu, 2009; Damm et al., 2012). Genomic DNA of three representative isolates (FAG14, FAG15 and FAG16) were extracted from 100 mg of fresh mycelium using the NucleoSpin®PlantII kit manufacturer's protocol for fungi (Macherey-Nagel,Germany). The ribosomal internal transcribed spacer (ITS), β-tubulin (TUB2), actin (ACT), partial chitin synthase (CHS-1), histone 3 (HIS3) and a 200-bp intron of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) regions were amplified and sequenced. A BLASTn search of the sequences (GenBank Accession Nos. OR587916-18 for ITS, OR596663-65 for TUB2, OR596666-68 for ACT, OR596669-71 for CHS-1, OR596672-74 for HIS3, OR596675:77 for GAPDH) revealed 100% identity with C. fioriniae. Pathogenicity tests were done using pure cultures of the FAG14 isolate. Healthy beech leaves were surface sterilised and inoculated with 10 μL of a spore suspension (106 conidia/mL) on the surface. Leaves inoculated with SDW were used as a control. The inoculated samples were placed in closed sterile boxes and kept at 25°C and 100% relative humidity. Seven to ten days after inoculation, the leaves had necrotic spots, whereas the controls remained healthy (Figure 4). To fulfil Koch's postulates, C. fioriniae was reisolated from the symptomatic leaves and its identity was confirmed both morphologically and molecularly. To the best of our knowledge, this pathogen has only previously been reported on Fagus sylvatica in seedlings in a nursery in Poland (Pszczółkowska et al., 2017). Thus, this is the first report of C. fioriniae causing anthracnose on adult trees of Fagus sylvatica in a natural forest in Italy. This study is part of the Agritech National Research Center and funded by the European Union Next-Generation EU.
欧洲山毛榉(Fagus sylvatica)是意大利和整个欧洲最常见的落叶森林物种之一，具有重要的经济、文化和环境价值(Siddique et al.， 2021)。2023年6月，从位于意大利中部维特博Oriolo Romano的联合国教科文组织遗址“Faggeta Vetusta di Monte Raschio”收集了显示炭疽症状的叶子(图1)。为确定病原菌，分别用70%乙醇浸泡2分钟、1%次氯酸钠浸泡3分钟、70%乙醇浸泡1分钟对叶片进行表面消毒，最后用无菌水(SDW)冲洗3次。用无菌手术刀获得的切片被镀在马铃薯葡萄糖琼脂板上，在25°C下孵育8至15天。发育中的真菌菌落具有白色至灰色的棉质菌丝，菌丝为淡橙色，背面有深色斑点(图2)。分生孢子透明，壁光滑，无菌丝，两端尖呈窄椭圆形，尺寸为10.5-21 μm(平均16.4 μm) × 3.5-7 μm(平均4.3 μm)(图3)。Damm et al.， 2012)。使用NucleoSpin®PlantII试剂盒制造商的真菌(Macherey-Nagel，德国)方案，从100 mg新鲜菌丝体中提取三个代表性分离株(FAG14、FAG15和FAG16)的基因组DNA。对核糖体内转录间隔段(ITS)、β-微管蛋白(TUB2)、肌动蛋白(ACT)、部分几丁质合成酶(CHS-1)、组蛋白3 (HIS3)和甘油醛-3-磷酸脱氢酶(GAPDH)区域的200 bp内含子进行扩增和测序。对序列进行BLASTn检索(GenBank Accession no . OR587916-18 for ITS, OR596663-65 for TUB2, OR596666-68 for ACT, OR596669-71 for CHS-1, OR596672-74 for HIS3, OR596675:77 for GAPDH)，结果显示与C. fioriniae的同源性为100%。采用FAG14分离物的纯培养物进行致病性试验。用10 μL孢子悬浮液(106个分生孢子/mL)接种健康山毛榉叶片。以接种SDW的叶片为对照。接种后的样品置于封闭无菌箱中，25℃、100%相对湿度保存。接种后7至10天，叶片出现坏死斑点，而对照组保持健康(图4)。为了满足Koch的假设，从有症状的叶片中重新分离出fioriniae，并在形态和分子上证实了其身份。据我们所知，这种病原体以前只在波兰一个苗圃的Fagus sylvatica幼苗上报道过(Pszczółkowska et al.， 2017)。因此，这是意大利天然林中首次报道菲罗氏弧菌在森林Fagus sylvatica成树上引起炭疽病。这项研究是Agritech国家研究中心的一部分，由欧盟下一代欧盟资助。
{"title":"First report of anthracnose on <i>Fagus sylvatica</i> caused by <i>Colletotrichum fioriniae</i> in Italy","authors":"I. Giubilei, F. Brugneti, S. Turco, M. I. Drais, A. Mazzaglia","doi":"10.1002/ndr2.12226","DOIUrl":"https://doi.org/10.1002/ndr2.12226","url":null,"abstract":"European beech (Fagus sylvatica) is one of the most common deciduous forest species in Italy and across Europe, important for its economic, cultural and environmental value (Siddique et al., 2021). In June 2023, leaves showing anthracnose symptoms (Figure 1) were collected from the UNESCO site “Faggeta Vetusta di Monte Raschio” located in Oriolo Romano, Viterbo in central Italy. To identify the causal agent, leaves were surface sterilised by dipping successively in 70% ethanol for two minutes, 1% sodium hypochlorite for three minutes and 70% ethanol for one minute, and finally rinsed three times with sterile water (SDW). Slices obtained with a sterile scalpel were plated on potato dextrose agar plates and incubated at 25°C for 8 to 15 days. Developing fungal colonies had white to grey cottony mycelium and were pale orange with dark flecking from the reverse side (Figure 2). Conidia were hyaline, smooth-walled, aseptate, narrowly elliptical pointed at both ends, measuring 10.5-21 μm (mean 16.4 μm) × 3.5-7 μm (mean 4.3 μm) (Figure 3). The morphological characteristics fit published descriptions of Colletotrichum fioriniae (Shivas & Yu, 2009; Damm et al., 2012). Genomic DNA of three representative isolates (FAG14, FAG15 and FAG16) were extracted from 100 mg of fresh mycelium using the NucleoSpin®PlantII kit manufacturer's protocol for fungi (Macherey-Nagel,Germany). The ribosomal internal transcribed spacer (ITS), β-tubulin (TUB2), actin (ACT), partial chitin synthase (CHS-1), histone 3 (HIS3) and a 200-bp intron of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) regions were amplified and sequenced. A BLASTn search of the sequences (GenBank Accession Nos. OR587916-18 for ITS, OR596663-65 for TUB2, OR596666-68 for ACT, OR596669-71 for CHS-1, OR596672-74 for HIS3, OR596675:77 for GAPDH) revealed 100% identity with C. fioriniae. Pathogenicity tests were done using pure cultures of the FAG14 isolate. Healthy beech leaves were surface sterilised and inoculated with 10 μL of a spore suspension (106 conidia/mL) on the surface. Leaves inoculated with SDW were used as a control. The inoculated samples were placed in closed sterile boxes and kept at 25°C and 100% relative humidity. Seven to ten days after inoculation, the leaves had necrotic spots, whereas the controls remained healthy (Figure 4). To fulfil Koch's postulates, C. fioriniae was reisolated from the symptomatic leaves and its identity was confirmed both morphologically and molecularly. To the best of our knowledge, this pathogen has only previously been reported on Fagus sylvatica in seedlings in a nursery in Poland (Pszczółkowska et al., 2017). Thus, this is the first report of C. fioriniae causing anthracnose on adult trees of Fagus sylvatica in a natural forest in Italy. This study is part of the Agritech National Research Center and funded by the European Union Next-Generation EU.","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136167596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. D. Takooree, H. Neetoo, V. M. Ranghoo‐Sanmukhiya
{"title":"First report of <i>Colletotrichum coccodes</i> causing black dot on potato in Mauritius","authors":"S. D. Takooree, H. Neetoo, V. M. Ranghoo‐Sanmukhiya","doi":"10.1002/ndr2.12224","DOIUrl":"https://doi.org/10.1002/ndr2.12224","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136169164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ouattara, T. A. Nana, K. H. Sogoba, K. Koïta, P. Lefeuvre, J. M. Lett
Pepper yellow vein disease (PYVD) has been reported to be associated with the African monopartite begomovirus Pepper yellow vein Mali virus (PepYVMLV) in West Africa (Tiendrébéogo et al., 2008; Zhou et al., 2008). Recently, vegetable isolates of PepYVMLV have also been shown to be associated with a DNA-B component in Burkina Faso (Ouattara et al., 2019) and Cote d'Ivoire (Soro et al., 2021). In September 2021, severe leaf yellowing, curling and deformation symptoms (Figure 1), resembling those of pepper yellow vein disease were observed on tobacco (Nicotiana tabacum) bordering pepper and tomato fields in the locality of Tabtenga in Burkina Faso. Tobacco leaves with (n = 3) and without (n = 2) symptoms were collected. The samples were tested for the presence of begomoviruses using a PCR assay with degenerate primers designed to amplify the coat protein gene of Old World begomoviruses (Séka et al., 2016) followed by direct sequencing of PCR amplicons. PCR products of the expected size were only obtained from the three diseased tobacco plants. BLASTn analyses of the amplicon sequences (676, 687 and 694 base pairs (bp) in length; GenBank Accession Nos. OR483371 - OR483373) showed the highest pairwise identity (98 to 99%) with PepYVMLV isolates from Burkina Faso (MH778652; MH778653). To confirm these results, specific primers for the detection of both DNA-A and -B components of PepYVMLV were used for PCR testing (Ouattara et al., 2019). Both components were detected from the three diseased samples confirming the initial diagnosis. To obtain the complete sequences of DNA-A and -B components, samples were subjected to nanopore MinION sequencing as described by Ben Chehida et al. (2021). Global similarity search analysis resulted in 84/3076 and 99/3076 raw reads assigned to PepYVMLV DNA-A and DNA-B, respectively. Based on sequence assembly only one contig of 2781 bp (DNA-A component) was obtained (OR483374) with 96.7% identity to PepYVMLV isolated from Burkina Faso (MH778653, FN555171). A maximum-likelihood phylogenetic tree constructed with publicly available begomovirus genome sequences and the sequences obtained in this study (OR483371 - OR483374) confirmed the genetic relationship of the isolates of PepYVMLV from tobacco with previously characterised isolates from West Africa (Figure 2). To our knowledge, this is the first report of PepYVMLV naturally associated with tobacco yellow leaf curl disease in Burkina Faso and globally. Our results highlight the potential existence of alternative natural hosts for PepYVMLV. This work was supported by The World Academy of Sciences, Trieste, Italy (TWAS) (Grant n°_21-147 RG/BIO/AF/AC_I-FR3240319486), the European Union (ERDF), the Conseil Régional de La Réunion and CIRAD, and conducted on the Plant Protection Platform (3P, IBISA). Dr Ouattara is a recipient of a 12-month visiting scientist fellowship of the program Make Our Planet Great Again (MOPGA-postdoc4-4610922765) from the French Ministry for Europe and
据报道，西非的辣椒黄静脉病(PYVD)与非洲单株begomvirus(非洲单株begomvirus)有关(tiendrsambsamogo等人，2008;Zhou et al.， 2008)。最近，在布基纳法索(Ouattara等人，2019)和科特迪瓦(Soro等人，2021)，PepYVMLV的植物分离株也被证明与DNA-B成分相关。2021年9月，在布基纳法索Tabtenga地区与辣椒和番茄田接壤的烟草(Nicotiana tabacum)上观察到类似辣椒黄脉病的严重叶片黄化、卷曲和变形症状(图1)。收集有(n = 3)和无(n = 2)症状的烟叶。采用聚合酶链反应(PCR)检测样品是否存在贝古莫病毒(ssamuka et al.， 2016)，并对PCR扩增子进行直接测序。聚合酶链反应引物设计用于扩增旧大陆贝古莫病毒外壳蛋白基因。预期大小的PCR产物仅从三株患病烟草植株中获得。扩增子序列(长度分别为676、687和694碱基对)的BLASTn分析;GenBank Accession no . OR483371 - OR483373)显示与布基纳法索PepYVMLV分离株(MH778652;MH778653)。为了证实这些结果，我们使用检测PepYVMLV DNA-A和-B成分的特异性引物进行PCR检测(Ouattara et al.， 2019)。从三个患病样本中检测到这两种成分，证实了初步诊断。为了获得DNA-A和-B组分的完整序列，样品进行了纳米孔MinION测序，如Ben Chehida等人(2021)所述。全球相似性搜索分析结果显示，84/3076和99/3076个原始reads分别归属于PepYVMLV DNA-A和DNA-B。基于序列组装，仅获得一个2781 bp (DNA-A组分)的序列(OR483374)，与布基纳法索分离的PepYVMLV (MH778653, FN555171)具有96.7%的同源性。利用公开获得的begomvirus基因组序列和本研究获得的序列(OR483371 - OR483374)构建的最大似然系统发育树证实了来自烟草的PepYVMLV分离株与以前从西非鉴定的分离株的遗传关系(图2)。据我们所知，这是布基纳法索和全球首次报道与烟草黄叶卷曲病自然相关的PepYVMLV。我们的结果强调了PepYVMLV可能存在替代的自然宿主。本工作由意大利的里雅斯特世界科学院(TWAS) (Grant n°_21-147 RG/BIO/AF/AC_I-FR3240319486)、欧盟(ERDF)、Conseil r区域de La runion和CIRAD支持，并在植物保护平台(3P, IBISA)上进行。瓦塔拉博士是法国欧洲和外交部(MEAE)“让我们的星球再次伟大”(MOPGA-postdoc4-4610922765)项目为期12个月的访问科学家奖学金获得者。
{"title":"First report of a naturally occurring isolate of <i>Pepper yellow vein Mali virus</i> causing tobacco yellow leaf curl disease in Burkina Faso","authors":"A. Ouattara, T. A. Nana, K. H. Sogoba, K. Koïta, P. Lefeuvre, J. M. Lett","doi":"10.1002/ndr2.12217","DOIUrl":"https://doi.org/10.1002/ndr2.12217","url":null,"abstract":"Pepper yellow vein disease (PYVD) has been reported to be associated with the African monopartite begomovirus Pepper yellow vein Mali virus (PepYVMLV) in West Africa (Tiendrébéogo et al., 2008; Zhou et al., 2008). Recently, vegetable isolates of PepYVMLV have also been shown to be associated with a DNA-B component in Burkina Faso (Ouattara et al., 2019) and Cote d'Ivoire (Soro et al., 2021). In September 2021, severe leaf yellowing, curling and deformation symptoms (Figure 1), resembling those of pepper yellow vein disease were observed on tobacco (Nicotiana tabacum) bordering pepper and tomato fields in the locality of Tabtenga in Burkina Faso. Tobacco leaves with (n = 3) and without (n = 2) symptoms were collected. The samples were tested for the presence of begomoviruses using a PCR assay with degenerate primers designed to amplify the coat protein gene of Old World begomoviruses (Séka et al., 2016) followed by direct sequencing of PCR amplicons. PCR products of the expected size were only obtained from the three diseased tobacco plants. BLASTn analyses of the amplicon sequences (676, 687 and 694 base pairs (bp) in length; GenBank Accession Nos. OR483371 - OR483373) showed the highest pairwise identity (98 to 99%) with PepYVMLV isolates from Burkina Faso (MH778652; MH778653). To confirm these results, specific primers for the detection of both DNA-A and -B components of PepYVMLV were used for PCR testing (Ouattara et al., 2019). Both components were detected from the three diseased samples confirming the initial diagnosis. To obtain the complete sequences of DNA-A and -B components, samples were subjected to nanopore MinION sequencing as described by Ben Chehida et al. (2021). Global similarity search analysis resulted in 84/3076 and 99/3076 raw reads assigned to PepYVMLV DNA-A and DNA-B, respectively. Based on sequence assembly only one contig of 2781 bp (DNA-A component) was obtained (OR483374) with 96.7% identity to PepYVMLV isolated from Burkina Faso (MH778653, FN555171). A maximum-likelihood phylogenetic tree constructed with publicly available begomovirus genome sequences and the sequences obtained in this study (OR483371 - OR483374) confirmed the genetic relationship of the isolates of PepYVMLV from tobacco with previously characterised isolates from West Africa (Figure 2). To our knowledge, this is the first report of PepYVMLV naturally associated with tobacco yellow leaf curl disease in Burkina Faso and globally. Our results highlight the potential existence of alternative natural hosts for PepYVMLV. This work was supported by The World Academy of Sciences, Trieste, Italy (TWAS) (Grant n°_21-147 RG/BIO/AF/AC_I-FR3240319486), the European Union (ERDF), the Conseil Régional de La Réunion and CIRAD, and conducted on the Plant Protection Platform (3P, IBISA). Dr Ouattara is a recipient of a 12-month visiting scientist fellowship of the program Make Our Planet Great Again (MOPGA-postdoc4-4610922765) from the French Ministry for Europe and ","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135606839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Leaf spot of Ficus maclellandii caused by Penicillium crocicola ‐ a first report from Iran","authors":"A. Esmaeilzadeh, D. Zafari, M. Ketabchi","doi":"10.1002/ndr2.12238","DOIUrl":"https://doi.org/10.1002/ndr2.12238","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139326660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. A. Fernández‐Ozuna, A. R. Gini Álvarez, H. Lopez-Nicora, A. A. Arrúa Alvarenga, A. A. Colmán
{"title":"First report of Neopestalotiopsis rosae causing leaf spot and crown rot on strawberry (Fragaria × ananassa) in Paraguay","authors":"Y. A. Fernández‐Ozuna, A. R. Gini Álvarez, H. Lopez-Nicora, A. A. Arrúa Alvarenga, A. A. Colmán","doi":"10.1002/ndr2.12239","DOIUrl":"https://doi.org/10.1002/ndr2.12239","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139328041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. M. Abo‐Elwafa, S. S. Ragab, Y. Nehela, T. A. Essa
{"title":"First report of strawberry anthracnose caused by Colletotrichum nymphaeae in Egypt","authors":"T. M. Abo‐Elwafa, S. S. Ragab, Y. Nehela, T. A. Essa","doi":"10.1002/ndr2.12205","DOIUrl":"https://doi.org/10.1002/ndr2.12205","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41574557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First report of Phytopythium vexans and Phytopythium litorale associated with root rot symptoms on red raspberry (Rubus idaeus)","authors":"E. Y. Browne, S. Edwards, C. Nellist","doi":"10.1002/ndr2.12197","DOIUrl":"https://doi.org/10.1002/ndr2.12197","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46694478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. McNally, K. Prapagar, K. Goldenhar, E. Pate, S. Shan, M. Kalischuk
{"title":"First report of an aggressive species of Neopestalotiopsis affecting strawberry in Canada","authors":"J. McNally, K. Prapagar, K. Goldenhar, E. Pate, S. Shan, M. Kalischuk","doi":"10.1002/ndr2.12210","DOIUrl":"https://doi.org/10.1002/ndr2.12210","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"59 5-6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50799754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First report of Xanthomonas euvesicatoria pv. euvesicatoria causing bacterial leaf spot in chilli pepper (Capsicum sp.) in Indonesia","authors":"D. Utami, N. N. S. Jayasanti, S. Meale, A. Young","doi":"10.1002/ndr2.12208","DOIUrl":"https://doi.org/10.1002/ndr2.12208","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49316581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In 2021, fifty-two watermelon (Citrullus lanatus) samples exhibiting yellowing virus-like symptoms (Figures 1, 2) were collected from thirteen open-field plots in Murcia (southeastern Spain). Total RNA extraction and dot-blot molecular hybridization were performed to detect Cucumber vein yellowing virus, Cucurbit aphid-borne yellows virus (CABYV), Cucurbit chlorotic yellows virus, Cucurbit yellow stunting disorder virus and Zucchini yellow mosaic virus (Rabadán et al., 2023), but none of these viruses were detected. Leaf yellowing symptoms may also be confused with plant nutrient deficiency. However, considering the large aphid populations in the affected crops and the inconspicuous symptoms that poleroviruses often cause in affected cucurbits, we sought to check for the possibility of polerovirus infection. Five random samples were subjected to RT-PCR using the polerovirus primers PolGenUp2 and PolGen RT3 (Lotos et al., 2014). For three samples, a 593 bp amplicon was produced, encompassing part of the polerovirus RNA-dependent RNA polymerase (RdRp) and sequenced (STAB VIDA, Portugal). The sequencing results from the three amplicons confirmed the presence of a polerovirus and identified the virus as Pepo aphid-borne yellows virus (PABYV), with 96.7% nucleic acid identity to GenBank Accession No. LN865082, reported from Greece. PABYV is a phloem-restricted RNA plant viruses that is transmitted by aphids and has been detected in West Africa and the Mediterranean, including Greece, Italy and Syria (Lotos et al., 2016; Parrella et al., 2023). To understand the extent and distribution of PABYV in these watermelon production areas, we amplified the coat protein (CP) from a Spanish PABYV isolate as described by Masika et al. (2022). The CP was cloned into the pGEMT-easy vector to facilitate the synthesis of a specific RNA-probe labeled with digoxigenin. All the watermelon samples collected in 2021, and a further 68 symptomatic samples collected during the 2022 and 2023 seasons, were tested for PABYV by dot-blot molecular hybridization, as described by De Moya-Ruiz et al. (2021). The analysis revealed that the occurrence of PABYV ranged from 75 to 100% in watermelon crops during the three seasons. Dot-blot hybridization was also used to detect the other cucurbit viruses listed above. Only PABYV was detected in 2021 and 2022, while PABYV and CABYV were found in mixed infections in all samples from 2023. Three PABYV isolates per year were selected randomly, and the CP gene was amplified, sequenced and deposited in GenBank (OR253793-OR253801). Phylogenetic analysis was done using the maximum likelihood method in MEGA X. The nine PABYV Spanish isolates clustered in a group along with the Greek (LN865082), Italian (OP973153) and Syrian (KC960436) isolates (Figure 3). This would suggest a common origin for the PABYV isolates spreading in the Mediterranean area. This is the first report of PABYV in Spain. The emergence of this aphid-borne virus in a new geograp
2021年，从穆尔西亚(西班牙东南部)的13个露天地块收集了52个西瓜(Citrullus lanatus)样品，表现出黄色病毒样症状(图1,2)。采用总RNA提取和点印迹分子杂交技术检测黄瓜静脉泛黄病毒、瓜蚜传黄病毒(CABYV)、瓜绿黄病毒、瓜黄发育障碍病毒和瓜黄花叶病毒(Rabadán et al.， 2023)，均未检测到这些病毒。叶片发黄症状也可能与植物营养缺乏相混淆。然而，考虑到受影响作物中的蚜虫种群数量大，以及受影响的瓜类中多病毒经常引起的不明显症状，我们试图检查多病毒感染的可能性。随机抽取5个样本，使用PolGenUp2和PolGen RT3引物进行RT-PCR (Lotos et al.， 2014)。对于三个样本，产生了一个593 bp的扩增子，包含部分多病毒RNA依赖性RNA聚合酶(RdRp)并进行了测序(STAB VIDA，葡萄牙)。测序结果证实了该病毒的存在，并鉴定该病毒为Pepo蚜虫传播的黄色病毒(PABYV)，其核酸识别率为96.7% (GenBank Accession No. 1)。LN865082，希腊报道。PABYV是一种韧皮部限制性RNA植物病毒，通过蚜虫传播，已在西非和地中海(包括希腊、意大利和叙利亚)检测到(Lotos等人，2016;Parrella et al.， 2023)。为了了解PABYV在这些西瓜产区的范围和分布，我们从Masika等人(2022)描述的西班牙PABYV分离物中扩增了外壳蛋白(CP)。将CP克隆到pGEMT-easy载体中，以促进合成以地高辛标记的特异性rna探针。按照De Moya-Ruiz等人(2021)的描述，采用点印迹分子杂交技术对2021年收集的所有西瓜样本以及2022年和2023年收集的68份有症状的西瓜样本进行了PABYV检测。分析表明，3个季节西瓜作物中PABYV的发生率为75% ~ 100%。斑点杂交也用于检测上面列出的其他葫芦病毒。仅在2021年和2022年检测到PABYV，而从2023年开始，在所有样本的混合感染中都发现了PABYV和CABYV。每年随机抽取3株PABYV分离株，扩增CP基因，测序并存入GenBank (OR253793-OR253801)。采用最大似然法对MEGA x进行系统发育分析。9株PABYV西班牙分离株与希腊(LN865082)、意大利(OP973153)和叙利亚(KC960436)分离株聚集在一起(图3)。这表明在地中海地区传播的PABYV分离株有一个共同的起源。这是西班牙首次报道PABYV。在一个新的地理区域出现这种蚜虫传播的病毒进一步证明，该病毒要么是最近在该地区传播，要么是在较长时间内存在，但在与其他病毒的混合感染中以前未报告和/或可能未被注意到。PABYV感染可能会减少葫芦产量。因此，在西班牙对PABYV进行系统和广泛的监测，以确定其在瓜类作物中的分布和流行情况至关重要。此外，对该病毒的生态学特征和生物学特性的进一步研究对了解其在地中海盆地的流行病学具有重要意义。本研究作为AGROALNEXT项目(PRTR-C17.l1)的一部分进行，由MCIN和Fundación ssamneca资助，由NextGenerationEU和CARM资助。CDMR在博士项目(SENECA 21417/FPI/20)中得到Fundación s逍遥的支持。
{"title":"First report of <i>Pepo aphid‐borne yellows virus</i> on watermelon plants in Spain","authors":"C. De Moya‐Ruiz, M. Juárez, P. Gómez","doi":"10.1002/ndr2.12215","DOIUrl":"https://doi.org/10.1002/ndr2.12215","url":null,"abstract":"In 2021, fifty-two watermelon (Citrullus lanatus) samples exhibiting yellowing virus-like symptoms (Figures 1, 2) were collected from thirteen open-field plots in Murcia (southeastern Spain). Total RNA extraction and dot-blot molecular hybridization were performed to detect Cucumber vein yellowing virus, Cucurbit aphid-borne yellows virus (CABYV), Cucurbit chlorotic yellows virus, Cucurbit yellow stunting disorder virus and Zucchini yellow mosaic virus (Rabadán et al., 2023), but none of these viruses were detected. Leaf yellowing symptoms may also be confused with plant nutrient deficiency. However, considering the large aphid populations in the affected crops and the inconspicuous symptoms that poleroviruses often cause in affected cucurbits, we sought to check for the possibility of polerovirus infection. Five random samples were subjected to RT-PCR using the polerovirus primers PolGenUp2 and PolGen RT3 (Lotos et al., 2014). For three samples, a 593 bp amplicon was produced, encompassing part of the polerovirus RNA-dependent RNA polymerase (RdRp) and sequenced (STAB VIDA, Portugal). The sequencing results from the three amplicons confirmed the presence of a polerovirus and identified the virus as Pepo aphid-borne yellows virus (PABYV), with 96.7% nucleic acid identity to GenBank Accession No. LN865082, reported from Greece. PABYV is a phloem-restricted RNA plant viruses that is transmitted by aphids and has been detected in West Africa and the Mediterranean, including Greece, Italy and Syria (Lotos et al., 2016; Parrella et al., 2023). To understand the extent and distribution of PABYV in these watermelon production areas, we amplified the coat protein (CP) from a Spanish PABYV isolate as described by Masika et al. (2022). The CP was cloned into the pGEMT-easy vector to facilitate the synthesis of a specific RNA-probe labeled with digoxigenin. All the watermelon samples collected in 2021, and a further 68 symptomatic samples collected during the 2022 and 2023 seasons, were tested for PABYV by dot-blot molecular hybridization, as described by De Moya-Ruiz et al. (2021). The analysis revealed that the occurrence of PABYV ranged from 75 to 100% in watermelon crops during the three seasons. Dot-blot hybridization was also used to detect the other cucurbit viruses listed above. Only PABYV was detected in 2021 and 2022, while PABYV and CABYV were found in mixed infections in all samples from 2023. Three PABYV isolates per year were selected randomly, and the CP gene was amplified, sequenced and deposited in GenBank (OR253793-OR253801). Phylogenetic analysis was done using the maximum likelihood method in MEGA X. The nine PABYV Spanish isolates clustered in a group along with the Greek (LN865082), Italian (OP973153) and Syrian (KC960436) isolates (Figure 3). This would suggest a common origin for the PABYV isolates spreading in the Mediterranean area. This is the first report of PABYV in Spain. The emergence of this aphid-borne virus in a new geograp","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135805127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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