Md Moshiur Rahman, Iva Alam Pinkey, Jannatul Ferthous, S. T. Arafat, S. M. Rahman, M. Asaduzzaman, Md. Mostafizur Rahman, M. Rouf
Climate change particularly global warming unceasingly imposes a selective pressure in many organisms that results in phenotypic plasticity particularly by expressing different adaptive phenotypes to shifting environmental conditions. The present study was, therefore, conducted to explore the phenotypic responses of male guppy ( Poecilia reticulata ), a popular model fish, to an ambient (28±0.91°C), high (32±0.12°C) and low (22±0.17°C) rearing temperature. Almost equal sized juvenile males were collected from the wild and reared up to 30 days maintaining necessary conditions similar among treatments except the water temperature. The findings revealed that high-temperature reared fish had significantly lower number of courtships, reduced survival, decreased body size, deformed body shape, limited colour patterns and reduced sperm bundle number than the ambient- and low-temperature groups. On the other hand, low-treatment males performed significantly higher number of courtships than ambient-temperature group, while ambient-temperature reared males possessed significantly higher body size and iridescent colour area than low- temperature treatment. Interestingly, the findings also revealed some trade-offs between traits under thermal-induced stressed conditions (i.e. both high and low temperatures). These findings elicit further information about the thermal condition dependent expression of phenotypic traits of fishes which infer about how fish species will adapt in the predicted changing aquatic environments because of unprecedented climate change.
{"title":"Modulation of phenotypic traits under different rearing temperatures: Experimental evidence in male guppy (Poecilia reticulata)","authors":"Md Moshiur Rahman, Iva Alam Pinkey, Jannatul Ferthous, S. T. Arafat, S. M. Rahman, M. Asaduzzaman, Md. Mostafizur Rahman, M. Rouf","doi":"10.22034/IJAB.V8I5.856","DOIUrl":"https://doi.org/10.22034/IJAB.V8I5.856","url":null,"abstract":"Climate change particularly global warming unceasingly imposes a selective pressure in many organisms that results in phenotypic plasticity particularly by expressing different adaptive phenotypes to shifting environmental conditions. The present study was, therefore, conducted to explore the phenotypic responses of male guppy ( Poecilia reticulata ), a popular model fish, to an ambient (28±0.91°C), high (32±0.12°C) and low (22±0.17°C) rearing temperature. Almost equal sized juvenile males were collected from the wild and reared up to 30 days maintaining necessary conditions similar among treatments except the water temperature. The findings revealed that high-temperature reared fish had significantly lower number of courtships, reduced survival, decreased body size, deformed body shape, limited colour patterns and reduced sperm bundle number than the ambient- and low-temperature groups. On the other hand, low-treatment males performed significantly higher number of courtships than ambient-temperature group, while ambient-temperature reared males possessed significantly higher body size and iridescent colour area than low- temperature treatment. Interestingly, the findings also revealed some trade-offs between traits under thermal-induced stressed conditions (i.e. both high and low temperatures). These findings elicit further information about the thermal condition dependent expression of phenotypic traits of fishes which infer about how fish species will adapt in the predicted changing aquatic environments because of unprecedented climate change.","PeriodicalId":36975,"journal":{"name":"International Journal of Aquatic Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91265377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Hashemian, S. Soltanian, M. akhlaghi, A. Gholamhosseini
Fish epidermis functions as the first defense barrier against physical, chemical, and biological stressors. However, the effects of hand-stripping on fish mucosal immune responses have been hardly investigated. The present study investigated the effects of stripping procedures on skin mucosal immune responses of rainbow trout ( Oncorhynchus mykiss ) breeders. The skin mucus was sampled from six male and six female trout before and one week after the stripping handling. The results showed that stripping had significant effect on all parameters except protease activity, but gender had a significant effect only on the mucosal protease and alkaline phosphatase activity. The results did not show any effect of the interaction between stripping and gender on mucosal lysozyme activity. The data revealed that enzymatic activities of alkaline phosphatase, lysozyme, esterase, as well as the total immunoglobulin level and bactericidal activity were significantly reduced in the skin mucus of fish one week after the stripping. The reduction of immune parameters in the skin mucus could be related to immunosuppression caused by stripping stress which, in turn, might have made the fish more susceptible to microbial infections and diseases. Therefore, care should be taken during stripping to minimize the manipulation stress.
{"title":"The effects of hand-stripping on some epidermal mucus immune parameters in rainbow trout, Oncorhynchus mykiss","authors":"F. Hashemian, S. Soltanian, M. akhlaghi, A. Gholamhosseini","doi":"10.22034/IJAB.V8I5.774","DOIUrl":"https://doi.org/10.22034/IJAB.V8I5.774","url":null,"abstract":"Fish epidermis functions as the first defense barrier against physical, chemical, and biological stressors. However, the effects of hand-stripping on fish mucosal immune responses have been hardly investigated. The present study investigated the effects of stripping procedures on skin mucosal immune responses of rainbow trout ( Oncorhynchus mykiss ) breeders. The skin mucus was sampled from six male and six female trout before and one week after the stripping handling. The results showed that stripping had significant effect on all parameters except protease activity, but gender had a significant effect only on the mucosal protease and alkaline phosphatase activity. The results did not show any effect of the interaction between stripping and gender on mucosal lysozyme activity. The data revealed that enzymatic activities of alkaline phosphatase, lysozyme, esterase, as well as the total immunoglobulin level and bactericidal activity were significantly reduced in the skin mucus of fish one week after the stripping. The reduction of immune parameters in the skin mucus could be related to immunosuppression caused by stripping stress which, in turn, might have made the fish more susceptible to microbial infections and diseases. Therefore, care should be taken during stripping to minimize the manipulation stress.","PeriodicalId":36975,"journal":{"name":"International Journal of Aquatic Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86880122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This work provides the revised and updated checklist of the seagrass in Sri Lanka based on primary and secondary information. It is indeed to be an update the list of Seagrass species in Sri Lanka prepared by Abeywickrama and Arulgnanam (1991), which now been nearly three decades back. More than forty (40) published pieces of literature, herbarium sheets in the National Herbarium and author’s personal observations since 2011 were reviewed. Sri Lanka belongs to the Indo-Pacific bioregion and 15 species have been recorded so far representing seven genera. They are Enhalus acoroides, Halophila beccarii , H. decipiens , H. ovalis , H. major , H. minor , H. ovata , H. stipulacea , Thalassia hemprichii , Cymodocea rotundata , C. serrulata , Halodule uninervis , H. pinifolia , Ruppia maritima , and Syringodium isoetifolium with the estimated extend to be 37,137 ha. Halophila beccarii considered as a rare species, and has only been recorded in patchily distributed with few localities in Sri Lanka. Lack of evidences to cross check the identification of H. minor (only recorded in Negombo Lagoon) and H. ovata is a main drawback. The composition and distribution of seagrasses along the coastal zone of Sri Lanka is yet to be established. Therefore, island wide comprehensive and genetic studies could be positively contributed to fulfil the data gaps in future.
{"title":"Current status and checklist of seagrass in Sri Lanka","authors":"S. Udagedara, D. Dahanayaka","doi":"10.22034/IJAB.V8I5.619","DOIUrl":"https://doi.org/10.22034/IJAB.V8I5.619","url":null,"abstract":"This work provides the revised and updated checklist of the seagrass in Sri Lanka based on primary and secondary information. It is indeed to be an update the list of Seagrass species in Sri Lanka prepared by Abeywickrama and Arulgnanam (1991), which now been nearly three decades back. More than forty (40) published pieces of literature, herbarium sheets in the National Herbarium and author’s personal observations since 2011 were reviewed. Sri Lanka belongs to the Indo-Pacific bioregion and 15 species have been recorded so far representing seven genera. They are Enhalus acoroides, Halophila beccarii , H. decipiens , H. ovalis , H. major , H. minor , H. ovata , H. stipulacea , Thalassia hemprichii , Cymodocea rotundata , C. serrulata , Halodule uninervis , H. pinifolia , Ruppia maritima , and Syringodium isoetifolium with the estimated extend to be 37,137 ha. Halophila beccarii considered as a rare species, and has only been recorded in patchily distributed with few localities in Sri Lanka. Lack of evidences to cross check the identification of H. minor (only recorded in Negombo Lagoon) and H. ovata is a main drawback. The composition and distribution of seagrasses along the coastal zone of Sri Lanka is yet to be established. Therefore, island wide comprehensive and genetic studies could be positively contributed to fulfil the data gaps in future.","PeriodicalId":36975,"journal":{"name":"International Journal of Aquatic Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82541395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was undertaken to evaluate the effects of apple cider vinegar on growth performance and non-specific immune parameters of skin mucus (alkaline phosphatase, lysozyme and total protein) in common carp fingerlings. For this purpose, a total of 240 fish were stocked in twelve tanks for four treatment with three replications and fed by diets supplemented with 0, 1, 2 and 4 % apple cider vinegar for 60 days. The result showed that there was no significant difference in body weight increase (BWI), feed conversion ratio (FCR), specific growth rate (SGR) and condition factor (CF) among the treatments ( P >0.05). The results revealed that there is no significant difference among skin mucus alkaline phosphatase of fish in all treatments. Although skin mucus lysozyme activity and total protein significantly increased by apple cider vinegar in comparison with the control group. The highest and lowest skin mucus lysozyme activity and total protein in common carp fingerlings were observed at 2% apple cider vinegar and control group, respectively. Therefore, apple cider vinegar improves skin mucus lysozyme activity and total protein and can be a good candidate for antibiotic replacement in common carp fingerlings.
{"title":"Effects of apple cider vinegar on growth performance and non-specific immune parameters of skin mucus in common carp (Cyprinus carpio) fingerlings","authors":"Hamed Nekoubin, A. Hajimoradloo, S. Hoseinifar","doi":"10.22034/IJAB.V8I5.818","DOIUrl":"https://doi.org/10.22034/IJAB.V8I5.818","url":null,"abstract":"This study was undertaken to evaluate the effects of apple cider vinegar on growth performance and non-specific immune parameters of skin mucus (alkaline phosphatase, lysozyme and total protein) in common carp fingerlings. For this purpose, a total of 240 fish were stocked in twelve tanks for four treatment with three replications and fed by diets supplemented with 0, 1, 2 and 4 % apple cider vinegar for 60 days. The result showed that there was no significant difference in body weight increase (BWI), feed conversion ratio (FCR), specific growth rate (SGR) and condition factor (CF) among the treatments ( P >0.05). The results revealed that there is no significant difference among skin mucus alkaline phosphatase of fish in all treatments. Although skin mucus lysozyme activity and total protein significantly increased by apple cider vinegar in comparison with the control group. The highest and lowest skin mucus lysozyme activity and total protein in common carp fingerlings were observed at 2% apple cider vinegar and control group, respectively. Therefore, apple cider vinegar improves skin mucus lysozyme activity and total protein and can be a good candidate for antibiotic replacement in common carp fingerlings.","PeriodicalId":36975,"journal":{"name":"International Journal of Aquatic Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88977188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study aimed to investigate the feeding ecology of a vulnerable freshwater puffer fish, Carinotetradon travancoricus from the Chalakudy river of Kerala, Western Ghats, a biodiversity hotspot of India. Fishes were sampled from October 2018 to September 2019. Stomach condition of the fishes showed the percentage of empty gut to be significantly higher during all seasons ( P < 0.01). Feeding intensity depicted the fish to follow an ‘active’ feeding strategy (31.59±10.32%) during pre-monsoon season. Diet composition and relative length of gut analysis indicated that the fish during its early stages relied on an omnivorous diet however preferring autochthonous food materials such as insects (27.91%) and crustaceans (25.30%) during its adult stages. A perceptible variation in the feeding strategy associated with the spawning season of C. travancoricus was also noticed. During their spawning season (May-August), a greater preponderance towards animal matter (52.18%) was noticed in their diet. The results of gastrosomatic index indicated that feeding activity of C. travancoricus is considerably reduced (2.99) during the spawning period. The present study provides the baseline information on the feeding ecology of C. travancoricus which could be helpful to aquarists for breeding and rearing of this species in captivity and thereby reducing their fishing pressure in wild.
{"title":"Feeding ecology of the endemic freshwater puffer fish Carinotetradon travancoricus (Hora & Nair, 1941) in Western Ghats hotspot, India","authors":"C. R. Renjithkumar, K. Roshni, K. Ranjeet","doi":"10.22034/IJAB.V8I5.922","DOIUrl":"https://doi.org/10.22034/IJAB.V8I5.922","url":null,"abstract":"The study aimed to investigate the feeding ecology of a vulnerable freshwater puffer fish, Carinotetradon travancoricus from the Chalakudy river of Kerala, Western Ghats, a biodiversity hotspot of India. Fishes were sampled from October 2018 to September 2019. Stomach condition of the fishes showed the percentage of empty gut to be significantly higher during all seasons ( P < 0.01). Feeding intensity depicted the fish to follow an ‘active’ feeding strategy (31.59±10.32%) during pre-monsoon season. Diet composition and relative length of gut analysis indicated that the fish during its early stages relied on an omnivorous diet however preferring autochthonous food materials such as insects (27.91%) and crustaceans (25.30%) during its adult stages. A perceptible variation in the feeding strategy associated with the spawning season of C. travancoricus was also noticed. During their spawning season (May-August), a greater preponderance towards animal matter (52.18%) was noticed in their diet. The results of gastrosomatic index indicated that feeding activity of C. travancoricus is considerably reduced (2.99) during the spawning period. The present study provides the baseline information on the feeding ecology of C. travancoricus which could be helpful to aquarists for breeding and rearing of this species in captivity and thereby reducing their fishing pressure in wild.","PeriodicalId":36975,"journal":{"name":"International Journal of Aquatic Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72482816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ibrahim, C. Hussein, Nahla Ibrahim, Moena Badran, Firas Alshawy, A. Ahmad
Minke whale, a member of the Sub order Mysticeti, was sighted stranded in the northern , and southern parts of eastern Mediterranean. This paper report stranding of a Minke whale in Banyas Coast of Syria, and fills the gap of the species distribution between the north and south of the eastern Mediterranean.
{"title":"First stranding event of a Minke Whale Calf, Balaenoptera acutorostrata Lacépède, 1804, in the Syrian Coast, Eastern Mediterranean","authors":"A. Ibrahim, C. Hussein, Nahla Ibrahim, Moena Badran, Firas Alshawy, A. Ahmad","doi":"10.22034/IJAB.V8I4.890","DOIUrl":"https://doi.org/10.22034/IJAB.V8I4.890","url":null,"abstract":"Minke whale, a member of the Sub order Mysticeti, was sighted stranded in the northern , and southern parts of eastern Mediterranean. This paper report stranding of a Minke whale in Banyas Coast of Syria, and fills the gap of the species distribution between the north and south of the eastern Mediterranean.","PeriodicalId":36975,"journal":{"name":"International Journal of Aquatic Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75178769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present investigation elucidates the synergistic effects of improvised ecological parameters and ripe papaya ( Carica papaya ) meal on skin pigmentation, growth performance and survival of Banded gourami, Trichogaster fasciata , under confined environment. A feeding trial of 60 days was done with initial length groups from 6.6 to 9.7 cm using five isonitrogenous experimental diets formulated by supplementing graded levels of carotenoids at 1 to 5% and a control without carotenoids. Two ecological parameters temperature and light intensity were elevated using artificial modulators. At the onset of the feeding trial, the total carotenoid concentration in fish muscle in both male (2.94±0.07 μg.g -1 ) and female (2.54±0.05 μg.g -1 ), respectively, which significantly increased, highest being in male (6.86±0.12 μg.g -1 ) and female (5.96±0.07μg.g -1 ) fishes fed with 4% papaya meal. Positive correlation, (0.98) in male and (0.97) female was observed between elevated levels of dietary carotenoids and body pigmentation which revealed that incorporation of dietary carotenoids resulted in a significant increase in total carotenoid concentration in chromatophores. Congenial effects were observed on body indices was revealed by positive correlation of weight (0.79) to elevated levels of carotenoid and 100% survival rate of the fishes. The feeding regimes showed ripe papaya meal as cheap natural colour enhancer source can be safely supplemented at 4% levels in the diets to increase the skin pigmentation and optimum conditioning in optimized captive environment having temperature range of 26-28 o C and light intensity 344.0-346 Lux, without any xenobiotic effect on Banded gourami.
{"title":"The effect of ripe papaya, Carica papaya, as natural carotenoids meal on body pigmentation and growth performance in banded gourami, Trichogaster fasciata","authors":"A. Das, S. P. Biswas","doi":"10.22034/IJAB.V8I2.782","DOIUrl":"https://doi.org/10.22034/IJAB.V8I2.782","url":null,"abstract":"The present investigation elucidates the synergistic effects of improvised ecological parameters and ripe papaya ( Carica papaya ) meal on skin pigmentation, growth performance and survival of Banded gourami, Trichogaster fasciata , under confined environment. A feeding trial of 60 days was done with initial length groups from 6.6 to 9.7 cm using five isonitrogenous experimental diets formulated by supplementing graded levels of carotenoids at 1 to 5% and a control without carotenoids. Two ecological parameters temperature and light intensity were elevated using artificial modulators. At the onset of the feeding trial, the total carotenoid concentration in fish muscle in both male (2.94±0.07 μg.g -1 ) and female (2.54±0.05 μg.g -1 ), respectively, which significantly increased, highest being in male (6.86±0.12 μg.g -1 ) and female (5.96±0.07μg.g -1 ) fishes fed with 4% papaya meal. Positive correlation, (0.98) in male and (0.97) female was observed between elevated levels of dietary carotenoids and body pigmentation which revealed that incorporation of dietary carotenoids resulted in a significant increase in total carotenoid concentration in chromatophores. Congenial effects were observed on body indices was revealed by positive correlation of weight (0.79) to elevated levels of carotenoid and 100% survival rate of the fishes. The feeding regimes showed ripe papaya meal as cheap natural colour enhancer source can be safely supplemented at 4% levels in the diets to increase the skin pigmentation and optimum conditioning in optimized captive environment having temperature range of 26-28 o C and light intensity 344.0-346 Lux, without any xenobiotic effect on Banded gourami.","PeriodicalId":36975,"journal":{"name":"International Journal of Aquatic Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74681132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Jimoh, A. A. Ayeloja, M. I. Abubakar, Y. Yusuf, M. O. Shittu, S. A. Abdulsalami
The effect of dietary inclusion of differently timed dry heat-treated Jatropha curcas on the growth and economic performance of Nile tilapia, Oreochromis niloticus , was evaluated in a 56-day feeding trial. Five isonitrogenous and isolipidic dietary treatments (35% crude protein and 10% crude lipid) were made consisting of soybean meal (control) which was replaced by J. curcas seed meal toasted either 5 min or 10 min at 20 and 40% to make other four test diets. A total of 225 juveniles of O. niloticus were acclimatized for a week, weighed and allotted into five dietary treatments. Each treatment was replicated three times with fifteen fish per replicate. Fish were fed 5% body weight on two equal proportions per day for 56 days. Growth data were collected at two-week intervals. The results from the study indicated that there was significant difference ( P 0.05) in the different growth and economic performance parameters of fish fed CTR and fish fed D520T (5 min toasted, 20%). There was significant reduction in haematological and biochemical parameters of the blood of O. niloticus fed the different dietary treatments containing J. curcas seed meal. Based on economic and physiologic performance, soybean meal in Nile tilapia diet could be replaced up to 40% by 5-minute toasted J. curcass seed meal.
{"title":"Toasted Jatropha curcas seed meal in Nile tilapia (Oreochromis niloticus) diet: Effect on growth, economic performance, haematology, serum biochemistry and liver histology","authors":"W. Jimoh, A. A. Ayeloja, M. I. Abubakar, Y. Yusuf, M. O. Shittu, S. A. Abdulsalami","doi":"10.22034/IJAB.V8I2.808","DOIUrl":"https://doi.org/10.22034/IJAB.V8I2.808","url":null,"abstract":"The effect of dietary inclusion of differently timed dry heat-treated Jatropha curcas on the growth and economic performance of Nile tilapia, Oreochromis niloticus , was evaluated in a 56-day feeding trial. Five isonitrogenous and isolipidic dietary treatments (35% crude protein and 10% crude lipid) were made consisting of soybean meal (control) which was replaced by J. curcas seed meal toasted either 5 min or 10 min at 20 and 40% to make other four test diets. A total of 225 juveniles of O. niloticus were acclimatized for a week, weighed and allotted into five dietary treatments. Each treatment was replicated three times with fifteen fish per replicate. Fish were fed 5% body weight on two equal proportions per day for 56 days. Growth data were collected at two-week intervals. The results from the study indicated that there was significant difference ( P 0.05) in the different growth and economic performance parameters of fish fed CTR and fish fed D520T (5 min toasted, 20%). There was significant reduction in haematological and biochemical parameters of the blood of O. niloticus fed the different dietary treatments containing J. curcas seed meal. Based on economic and physiologic performance, soybean meal in Nile tilapia diet could be replaced up to 40% by 5-minute toasted J. curcass seed meal.","PeriodicalId":36975,"journal":{"name":"International Journal of Aquatic Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85950735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Choubkar, H. Farahmand, A. Mirvaghefi, H. Alizadeh, A. A. Elmdoust
In the present study, we evaluated the effect of commercial immunogenic prebiotic on the rate of methylation of type-C gene. A total of 120 rainbow trout were divided into two treatments, including a control group and another one with 2.0% commercial prebiotic immunogen each in three replicates. On the first, 15 and 45 days, DNA of adrenal tissue was extracted and treated with bisulfite. Samples were amplified by polymerase chain reaction (PCR) and sequenced. Based on the results, there was no significant difference ( P <0.05) between the 1st day (R), 15 th day of control (C), 15 th day of immunogen (I) and 40 th day of control (CS). However, there was a significant difference ( P <0.05) between the 45 th day immunogenicity (IS) and other samples, i.e. the CpG islet methylation rate in the IS samples was lower leading to increase in the expression of the lysozyme gene
{"title":"Methylation levels of lysozyme gene in rainbow trout (Oncorhynchus mykiss) fed by commercial immunogen probiotic","authors":"Ali Choubkar, H. Farahmand, A. Mirvaghefi, H. Alizadeh, A. A. Elmdoust","doi":"10.22034/IJAB.V8I2.881","DOIUrl":"https://doi.org/10.22034/IJAB.V8I2.881","url":null,"abstract":"In the present study, we evaluated the effect of commercial immunogenic prebiotic on the rate of methylation of type-C gene. A total of 120 rainbow trout were divided into two treatments, including a control group and another one with 2.0% commercial prebiotic immunogen each in three replicates. On the first, 15 and 45 days, DNA of adrenal tissue was extracted and treated with bisulfite. Samples were amplified by polymerase chain reaction (PCR) and sequenced. Based on the results, there was no significant difference ( P <0.05) between the 1st day (R), 15 th day of control (C), 15 th day of immunogen (I) and 40 th day of control (CS). However, there was a significant difference ( P <0.05) between the 45 th day immunogenicity (IS) and other samples, i.e. the CpG islet methylation rate in the IS samples was lower leading to increase in the expression of the lysozyme gene","PeriodicalId":36975,"journal":{"name":"International Journal of Aquatic Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82327767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hamid Rajabiesterabadi, A. Ghelichi, S. Jorjani, Seyyed Morteza Hoseini, R. Akrami
This study aimed to investigate the effects of dietary olive leaf extract (OLE) on intestinal immune-related genes expression of tumor necrosis factor alpha (TNFa), interleukin 1 beta (IL1b), lysozyme (LYS), and mucin2 (MUC2). For this purpose, common carp (~15 g) were fed with 0 (control), 0.1 (OLE-0.1), 0.5 (OLE-0.5) and 1 (OLE-1) % OLE diets for eight weeks. The fish were sampled after one and eight weeks to study intestinal TNFa, IL1b, LYS, and MUC2 gene expressions. The results showed that dietary OLE administration significantly up-regulated intestinal TNFa gene expression after one (all OLE-treated groups) and eight (OLE-0.5) weeks. Moreover, OLE-0.1 and OLE-1 groups showed up-regulated intestinal IL1b expression, after one week, all the OLE-treated fish had significantly higher intestinal IL1b expression, after eight weeks. OLE had no significant effects on LYS gene expression after one week, but OLE-0.1 and OLE-0.5 had significantly higher gene expressions after eight weeks. OLE-0.1 and OLE-1 had significantly lower MUC2 gene expression after one week, but all OLE-treated fish had significantly higher MUC2 gene expression after eight weeks. In conclusion, dietary 0.1-0.5% OLE supplementation is suitable to support common carp intestinal health.
{"title":"Effects of dietary olive leaf extract on intestinal immune-related gene expressions in common carp, Cyprinus carpio","authors":"Hamid Rajabiesterabadi, A. Ghelichi, S. Jorjani, Seyyed Morteza Hoseini, R. Akrami","doi":"10.22034/IJAB.V8I2.839","DOIUrl":"https://doi.org/10.22034/IJAB.V8I2.839","url":null,"abstract":"This study aimed to investigate the effects of dietary olive leaf extract (OLE) on intestinal immune-related genes expression of tumor necrosis factor alpha (TNFa), interleukin 1 beta (IL1b), lysozyme (LYS), and mucin2 (MUC2). For this purpose, common carp (~15 g) were fed with 0 (control), 0.1 (OLE-0.1), 0.5 (OLE-0.5) and 1 (OLE-1) % OLE diets for eight weeks. The fish were sampled after one and eight weeks to study intestinal TNFa, IL1b, LYS, and MUC2 gene expressions. The results showed that dietary OLE administration significantly up-regulated intestinal TNFa gene expression after one (all OLE-treated groups) and eight (OLE-0.5) weeks. Moreover, OLE-0.1 and OLE-1 groups showed up-regulated intestinal IL1b expression, after one week, all the OLE-treated fish had significantly higher intestinal IL1b expression, after eight weeks. OLE had no significant effects on LYS gene expression after one week, but OLE-0.1 and OLE-0.5 had significantly higher gene expressions after eight weeks. OLE-0.1 and OLE-1 had significantly lower MUC2 gene expression after one week, but all OLE-treated fish had significantly higher MUC2 gene expression after eight weeks. In conclusion, dietary 0.1-0.5% OLE supplementation is suitable to support common carp intestinal health.","PeriodicalId":36975,"journal":{"name":"International Journal of Aquatic Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89960259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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