Pub Date : 2022-09-21DOI: 10.33997/j.afs.2022.35.3.004
R. Razali, N. I. Mustapa, Wan Norhana Md. Noordin, M. A. Rahim, K. Hii, P. Lim, C. Leaw, H. Muhd-Farouk, K. Yaacob
A fish kill incident was reported at the marine fish culture areas north of Perak and south of Penang, Malaysia, on 26 May 2020. An investigation was carried out at 10 stations in Kerian, Perak and Seberang Perai Selatan, Penang. Seawater samples were collected to identify microalgae species and determination of dissolved inorganic nutrients. The physical parameters of water such as salinity, pH, temperature and dissolved oxygen were measured in situ while the dissolved inorganic nutrients were analysed spectrophotometrically. The dominant microalga was identified as Margalefidinium fulvescens (Iwataki, Kawami & Matsuoka) Gómez, Richlen & Anderson, 2017, based on the morphological and molecular characterisation of the large subunit ribosomal gene. Long rounded and ellipsoidal cells, 30-43 µm in length, appeared in chains of single, two, four or eight cells. The sulcus was slightly narrow surrounding the cell about one turn, but the cingulum was rather deep, encircling the cell approximately twice, and the chloroplasts were brownish, granular and scattered peripherally. The highest M. fulvescens cell counts were recorded at 6.22 × 105 cells L-1 and 4.61 × 105 cells L−1 in Kerian, Perak and Seberang Perai Selatan, Penang, respectively. The physical parameters of the seawater from the affected sites were within the Malaysian Marine Water Quality Standard (MMWQS) for aquaculture. However, slightly higher levels of nitrate, phosphate and ammonia were noted at several stations. Although the exact cause of the bloom was undecided, it could be due to nutrient discharge along the coasts, which also concurred with the transition phase of the northeast to the southwest monsoon.
{"title":"Report of a Fish Kill Due to a Dinoflagellate Bloom in Perak and Penang, Malaysia","authors":"R. Razali, N. I. Mustapa, Wan Norhana Md. Noordin, M. A. Rahim, K. Hii, P. Lim, C. Leaw, H. Muhd-Farouk, K. Yaacob","doi":"10.33997/j.afs.2022.35.3.004","DOIUrl":"https://doi.org/10.33997/j.afs.2022.35.3.004","url":null,"abstract":"A fish kill incident was reported at the marine fish culture areas north of Perak and south of Penang, Malaysia, on 26 May 2020. An investigation was carried out at 10 stations in Kerian, Perak and Seberang Perai Selatan, Penang. Seawater samples were collected to identify microalgae species and determination of dissolved inorganic nutrients. The physical parameters of water such as salinity, pH, temperature and dissolved oxygen were measured in situ while the dissolved inorganic nutrients were analysed spectrophotometrically. The dominant microalga was identified as Margalefidinium fulvescens (Iwataki, Kawami & Matsuoka) Gómez, Richlen & Anderson, 2017, based on the morphological and molecular characterisation of the large subunit ribosomal gene. Long rounded and ellipsoidal cells, 30-43 µm in length, appeared in chains of single, two, four or eight cells. The sulcus was slightly narrow surrounding the cell about one turn, but the cingulum was rather deep, encircling the cell approximately twice, and the chloroplasts were brownish, granular and scattered peripherally. The highest M. fulvescens cell counts were recorded at 6.22 × 105 cells L-1 and 4.61 × 105 cells L−1 in Kerian, Perak and Seberang Perai Selatan, Penang, respectively. The physical parameters of the seawater from the affected sites were within the Malaysian Marine Water Quality Standard (MMWQS) for aquaculture. However, slightly higher levels of nitrate, phosphate and ammonia were noted at several stations. Although the exact cause of the bloom was undecided, it could be due to nutrient discharge along the coasts, which also concurred with the transition phase of the northeast to the southwest monsoon.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48917855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-07DOI: 10.33997/j.afs.2022.35.3.003
G. Kibria
This review provides an account of fish species contaminated with microplastics (MPs) across the globe (seven continents). A total of 887 fish species were found contaminated with MPs based on MPs in the gastrointestinal tract/GI. The most MPs contaminated-fish species found were marine and demersal species. Globally 45 % of fish ingested MPs with an average concentration of 5.93 MPs particles per fish species. Among all the countries, China had the highest number of fish species contaminated with MPs in the followingorders: China (176 species), Brazil (84), the USA (48), India (35), the Atlantic Ocean (31), Iran (30), Bangladesh (28), Turkey (26), Indonesia (25), the UK (23), Saudi Arabia (23), Thailand (21), Portugal (20), Australia (20), Italy (18), South Africa (18), Argentina (15), Chile (14), Galapagos Islands (Ecuador) (14), the North Pacific Gyre (14), Samoa (13), Malaysia (12), Colombia (11), New Zealand (11), Fiji (10), Spain (10), the North Sea (09), South Korea (09), Tahiti (09), Vanuatu (09), Ghana (08), Canada (07), Japan (07) and Nigeria (07) and others. MPs ingestion in fishes varied (high, medium, and low) among the locations/countries. In several locations/countries, MPs ingestion/contamination occurred in up to 100 % of fish samples. Because of MPs contamination, seafood fisheries, and the livelihoods of people associated with fishing, aquaculture, and seafood business, can be threatened. It may also increase health risks to seafood fish consumers since there is a probability that high risks pollutants adsorbed in MPs can be transferred to humans via the food chain.
{"title":"Global Review and Analysis of the Presence of Microplastics in Fish","authors":"G. Kibria","doi":"10.33997/j.afs.2022.35.3.003","DOIUrl":"https://doi.org/10.33997/j.afs.2022.35.3.003","url":null,"abstract":"This review provides an account of fish species contaminated with microplastics (MPs) across the globe (seven continents). A total of 887 fish species were found contaminated with MPs based on MPs in the gastrointestinal tract/GI. The most MPs contaminated-fish species found were marine and demersal species. Globally 45 % of fish ingested MPs with an average concentration of 5.93 MPs particles per fish species. Among all the countries, China had the highest number of fish species contaminated with MPs in the followingorders: China (176 species), Brazil (84), the USA (48), India (35), the Atlantic Ocean (31), Iran (30), Bangladesh (28), Turkey (26), Indonesia (25), the UK (23), Saudi Arabia (23), Thailand (21), Portugal (20), Australia (20), Italy (18), South Africa (18), Argentina (15), Chile (14), Galapagos Islands (Ecuador) (14), the North Pacific Gyre (14), Samoa (13), Malaysia (12), Colombia (11), New Zealand (11), Fiji (10), Spain (10), the North Sea (09), South Korea (09), Tahiti (09), Vanuatu (09), Ghana (08), Canada (07), Japan (07) and Nigeria (07) and others. MPs ingestion in fishes varied (high, medium, and low) among the locations/countries. In several locations/countries, MPs ingestion/contamination occurred in up to 100 % of fish samples. Because of MPs contamination, seafood fisheries, and the livelihoods of people associated with fishing, aquaculture, and seafood business, can be threatened. It may also increase health risks to seafood fish consumers since there is a probability that high risks pollutants adsorbed in MPs can be transferred to humans via the food chain.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43637075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-29DOI: 10.33997/j.afs.2022.35.3.002
Hoda Mohamed Lotfy Abdallah, S. Mohamedy, Abdallah Said Hamed, M. Bakry, M. Abdelal, Soad Mekawy
Bisphenol A (BPA) is one of the most widely used synthetic compounds in the world. Since BPA is a suspected xenoestrogen and oxidative stressful agent, its potential hazardous impacts were evaluated on the health and the reproductive status of the male North African catfish, Clarias gariepinus (Burchell, 1822). Ninety mature male North African catfish were divided into six groups (in three replicates); Group 1 & Group 4 fed basal diet (control), Group 2 & Group 5 diets supplemented with 3 % purslane, Portulaca oleracea Linnaeus, 1753 powder, and Group 3 & Group 6 diets supplemented with 5 % purslane powder. Group 4, Group 5, and Group 6 were exposed to 50 µg.L-1 BPA for 14 days. Group 4 showed a significant increase in the luteinising hormone, 17β estradiol, and malondialdehyde levels, with a decrease in the testosterone, superoxide dismutase, and catalase concentrations. Simultaneously, there were elevated liver and kidney markers with severe degenerative changes in the testes, liver, and kidney. Dietary supplementation of purslane powder returned the measured parameters to their normal values with the correction of the tissue architectures and details. Interestingly, purslane in the non-BPA treated groups raised the testosterone and 17β estradiol over the control values. It is suggested that purslane could be used in aquaculture and cultivated near polluted water areas.
{"title":"Diet Supplemented With Purslane, Portulaca oleracea Linnaeus, 1753 Resolves Bisphenol A Impact on North African Catfish, Clarias gariepinus (Burchell, 1822)","authors":"Hoda Mohamed Lotfy Abdallah, S. Mohamedy, Abdallah Said Hamed, M. Bakry, M. Abdelal, Soad Mekawy","doi":"10.33997/j.afs.2022.35.3.002","DOIUrl":"https://doi.org/10.33997/j.afs.2022.35.3.002","url":null,"abstract":"Bisphenol A (BPA) is one of the most widely used synthetic compounds in the world. Since BPA is a suspected xenoestrogen and oxidative stressful agent, its potential hazardous impacts were evaluated on the health and the reproductive status of the male North African catfish, Clarias gariepinus (Burchell, 1822). Ninety mature male North African catfish were divided into six groups (in three replicates); Group 1 & Group 4 fed basal diet (control), Group 2 & Group 5 diets supplemented with 3 % purslane, Portulaca oleracea Linnaeus, 1753 powder, and Group 3 & Group 6 diets supplemented with 5 % purslane powder. Group 4, Group 5, and Group 6 were exposed to 50 µg.L-1 BPA for 14 days. Group 4 showed a significant increase in the luteinising hormone, 17β estradiol, and malondialdehyde levels, with a decrease in the testosterone, superoxide dismutase, and catalase concentrations. Simultaneously, there were elevated liver and kidney markers with severe degenerative changes in the testes, liver, and kidney. Dietary supplementation of purslane powder returned the measured parameters to their normal values with the correction of the tissue architectures and details. Interestingly, purslane in the non-BPA treated groups raised the testosterone and 17β estradiol over the control values. It is suggested that purslane could be used in aquaculture and cultivated near polluted water areas.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45354214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-15DOI: 10.33997/j.afs.2022.35.3.001
V. Vinodha, A. Kalarani, R. Moses Inbaraj
Fourfinger threadfin, Eleutheronema tetradactylum (Shaw, 1804), is one of the country’s most popular table fish species. This is a priority species for mariculture in India. Reproductive biology, life cycle, culture, and management are some budding research areas, while there is a lacuna in the species' genetic information. Using Illumina next-generation sequencing technique, 82,072 mRNA transcripts in the mature testis of fourfinger threadfin were obtained. The present study attempted to understand the expression of genes that facilitate sex determination, sex differentiation and gonadal maturation. From 82,072 transcripts, 50,943 were predicted by comparing them to proteins in UniProt using BLASTX. A total of 41 genes involved in sex determination and sex differentiation, spermatogenesis, steroid receptors in testis, steroidogenesis and gonadotropin-releasing hormone (GnRH) regulation are reported for the first time in E. tetradactylum. The entire raw sequencing reads of transcripts were made available in NCBI under BioProject PRJNA770837.
{"title":"Transcriptome Sequencing and Analysis of Male Fourfinger Threadfin, Eleutheronema tetradactylum (Shaw, 1804)","authors":"V. Vinodha, A. Kalarani, R. Moses Inbaraj","doi":"10.33997/j.afs.2022.35.3.001","DOIUrl":"https://doi.org/10.33997/j.afs.2022.35.3.001","url":null,"abstract":"Fourfinger threadfin, Eleutheronema tetradactylum (Shaw, 1804), is one of the country’s most popular table fish species. This is a priority species for mariculture in India. Reproductive biology, life cycle, culture, and management are some budding research areas, while there is a lacuna in the species' genetic information. Using Illumina next-generation sequencing technique, 82,072 mRNA transcripts in the mature testis of fourfinger threadfin were obtained. The present study attempted to understand the expression of genes that facilitate sex determination, sex differentiation and gonadal maturation. From 82,072 transcripts, 50,943 were predicted by comparing them to proteins in UniProt using BLASTX. A total of 41 genes involved in sex determination and sex differentiation, spermatogenesis, steroid receptors in testis, steroidogenesis and gonadotropin-releasing hormone (GnRH) regulation are reported for the first time in E. tetradactylum. The entire raw sequencing reads of transcripts were made available in NCBI under BioProject PRJNA770837.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43446472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-29DOI: 10.33997/j.afs.2022.35.2.006
Tran Thi Thu Duyen, Nguyen Trong Tuan, T. Hoa
Crude methanol extracts of 43 Vietnamese plants were screened in vitro for their antibacterial activity against three common freshwater fish pathogens, including Aeromonas hydrophila, Edwardsiella ictaluri and Streptococcus agalactiae. The agar disc diffusion method was used to evaluate the antibacterial activity, followed by minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined using the broth dilution method. Among the 43 plants screened, eight plant extracts (Bouea oppositifolia, Wedelia chinensis, Terminalia catappa, Punica granatum, Sonneratia caseolaris, Sonneratia ovata, Ludwigia hyssopifolia and Phyllanthus urinaria) exhibited wide-spectrum antibacterial activity to all three common freshwater fish pathogens. Six plant extracts (Ageratum conyzoides, Alpinia galanga, Borassus flabellifer, Abutilon indicum, Eupatorium odotatum, and Scoparia dulcis) might be good candidates for the prevention of co-infection of E. ictaluri and S. agalactiae in tilapia whereas Muntingia calabura and Camellia sinensis could be applied to striped catfish to combat E. ictaluri and A. hydrophila. Through MIC and MBC determination, L. hyssopifolia, A. galanga, Ageratum conyzoides extracts showed a bactericidal activity to A. hydrophila, E. ictuluri and S. agalactiae, respectively, while the other extracts could prevent the growth of tested bacteria. The screening results suggested the potential application of plant extracts as alternative therapeutic agents against bacterial infections in aquaculture.
{"title":"In Vitro Antibacterial Activity of Tropical Plant Extracts Against Fish Pathogens in Vietnam","authors":"Tran Thi Thu Duyen, Nguyen Trong Tuan, T. Hoa","doi":"10.33997/j.afs.2022.35.2.006","DOIUrl":"https://doi.org/10.33997/j.afs.2022.35.2.006","url":null,"abstract":"Crude methanol extracts of 43 Vietnamese plants were screened in vitro for their antibacterial activity against three common freshwater fish pathogens, including Aeromonas hydrophila, Edwardsiella ictaluri and Streptococcus agalactiae. The agar disc diffusion method was used to evaluate the antibacterial activity, followed by minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined using the broth dilution method. Among the 43 plants screened, eight plant extracts (Bouea oppositifolia, Wedelia chinensis, Terminalia catappa, Punica granatum, Sonneratia caseolaris, Sonneratia ovata, Ludwigia hyssopifolia and Phyllanthus urinaria) exhibited wide-spectrum antibacterial activity to all three common freshwater fish pathogens. Six plant extracts (Ageratum conyzoides, Alpinia galanga, Borassus flabellifer, Abutilon indicum, Eupatorium odotatum, and Scoparia dulcis) might be good candidates for the prevention of co-infection of E. ictaluri and S. agalactiae in tilapia whereas Muntingia calabura and Camellia sinensis could be applied to striped catfish to combat E. ictaluri and A. hydrophila. Through MIC and MBC determination, L. hyssopifolia, A. galanga, Ageratum conyzoides extracts showed a bactericidal activity to A. hydrophila, E. ictuluri and S. agalactiae, respectively, while the other extracts could prevent the growth of tested bacteria. The screening results suggested the potential application of plant extracts as alternative therapeutic agents against bacterial infections in aquaculture.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49077414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-28DOI: 10.33997/j.afs.2022.35.2.005
Matine Chhorn, Thuy‐Yen Duong
This study identified Phalacronotus to species level and examined its morphometric variation among three collection localities, and between sexes, in the Mekong Delta, Vietnam. Specimens were collected from local markets and fishers, including An Giang (n = 36) and Can Tho (n = 36) in the Hau River and Tien Giang (n = 30) in the Tien River for morphological measurements and DNA barcoding analysis. Eight mitochondrial cytochrome oxidase subunit I (COI) sequences of Phalacronotus sp. shared 99.6 ± 0.23 % similarity with five GenBank (GB) P. bleekeri sequences and differed by 7.6 % to 13.3 % from seven GB P. apogon sequences. The Vietnamese specimens of Phalacronotus were similar to P. bleekeri in number of anal fin rays (75–80) and the shape of the upper jaw teeth. Thus, the COI sequences and these morphological characteristics indicate that Phalacronotus sp. samples collected in the Mekong Delta are Phalacronotus bleekeri (Günther, 1864). Morphometric characteristics differed between populations in 15 of 19 indices (P < 0.05) and between females and males in three characteristics relating to the belly. Head characteristics, body depth, and caudal peduncle depth were the main parameters contributing to the inter-population variation. Based on size-adjusted data, 96.7 % of specimens were correctly classified from the Tien Giang population, while 27.8 % and 33.3 % were misclassified between the Can Tho and An Giang populations, respectively. These results indicate that morphometric parameters of P. bleekeri varied mainly between populations in the two Mekong River tributaries.
{"title":"Identification of Phalacronotus bleekeri (Günther, 1864) (Siluridae) From the Mekong Delta, Vietnam and Use of Morphological Analysis to Separate Populations","authors":"Matine Chhorn, Thuy‐Yen Duong","doi":"10.33997/j.afs.2022.35.2.005","DOIUrl":"https://doi.org/10.33997/j.afs.2022.35.2.005","url":null,"abstract":"This study identified Phalacronotus to species level and examined its morphometric variation among three collection localities, and between sexes, in the Mekong Delta, Vietnam. Specimens were collected from local markets and fishers, including An Giang (n = 36) and Can Tho (n = 36) in the Hau River and Tien Giang (n = 30) in the Tien River for morphological measurements and DNA barcoding analysis. Eight mitochondrial cytochrome oxidase subunit I (COI) sequences of Phalacronotus sp. shared 99.6 ± 0.23 % similarity with five GenBank (GB) P. bleekeri sequences and differed by 7.6 % to 13.3 % from seven GB P. apogon sequences. The Vietnamese specimens of Phalacronotus were similar to P. bleekeri in number of anal fin rays (75–80) and the shape of the upper jaw teeth. Thus, the COI sequences and these morphological characteristics indicate that Phalacronotus sp. samples collected in the Mekong Delta are Phalacronotus bleekeri (Günther, 1864). Morphometric characteristics differed between populations in 15 of 19 indices (P < 0.05) and between females and males in three characteristics relating to the belly. Head characteristics, body depth, and caudal peduncle depth were the main parameters contributing to the inter-population variation. Based on size-adjusted data, 96.7 % of specimens were correctly classified from the Tien Giang population, while 27.8 % and 33.3 % were misclassified between the Can Tho and An Giang populations, respectively. These results indicate that morphometric parameters of P. bleekeri varied mainly between populations in the two Mekong River tributaries.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43491198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-19DOI: 10.33997/j.afs.2022.35.2.004
L. T. Dang, Hanh Nguyen, Yen Pham, H. Truong
Aeromonas spp., which inhabits freshwater and marine water bodies, can be responsible for the diseases and mortalities of many different cultured fishes. In this study, the antibiotic susceptibility profiles of 20 Aeromonas hydrophila strains isolated from diseased freshwater fish cultured in the Red River Delta, Vietnam were categorised as non-wild-type (non-WT) strains or were resistant to at least one antibiotic. Also, in-vitro antibacterial activities of extracts from two local plants against several antibiotic-resistant A. hydrophila strains were done to screen for potential bio-antibiotic materials. The antibiotic susceptibility results showed that 25 % of bacterial strains were resistant to 3–9 antibiotics, 35 % to 2 antibiotics, and 10 % to one antibiotic. Both plants, rose myrtle seed, Rhodomyrtus tomentosa Hassk, 1842, extract and fermented garlic, Allium sativum Linnaeus, 1753, supernatant, showed inhibitory activities against antibiotic-resistant A. hydrophila strains. Furthermore, the R. tomentosa extract and the fermented A. sativum supernatant exhibited significant antibacterial effects to several A. hydrophila strains, namely A. hydrophila CEDMA17.021, CEDMA17.002, CEDMA17.008, and CEDMA17.009 resistant to two or more antibiotics. This study demonstrated multiple resistant profiles of A. hydrophila strains to different antibiotics and the inhibitory activities of R. tomentosa extract and the fermented A. sativum supernatant against antibiotic-resistant A. hydrophila strains. Hence, this study indicates the potential use of bio-antibiotics derived from plants to manage Aeromonas-related infections.
{"title":"Antibiotic Susceptibility Profiles of Aeromonas hydrophila Isolates From Aquaculture Farms and Response to Potential Antibacterial Plant Extracts","authors":"L. T. Dang, Hanh Nguyen, Yen Pham, H. Truong","doi":"10.33997/j.afs.2022.35.2.004","DOIUrl":"https://doi.org/10.33997/j.afs.2022.35.2.004","url":null,"abstract":"Aeromonas spp., which inhabits freshwater and marine water bodies, can be responsible for the diseases and mortalities of many different cultured fishes. In this study, the antibiotic susceptibility profiles of 20 Aeromonas hydrophila strains isolated from diseased freshwater fish cultured in the Red River Delta, Vietnam were categorised as non-wild-type (non-WT) strains or were resistant to at least one antibiotic. Also, in-vitro antibacterial activities of extracts from two local plants against several antibiotic-resistant A. hydrophila strains were done to screen for potential bio-antibiotic materials. The antibiotic susceptibility results showed that 25 % of bacterial strains were resistant to 3–9 antibiotics, 35 % to 2 antibiotics, and 10 % to one antibiotic. Both plants, rose myrtle seed, Rhodomyrtus tomentosa Hassk, 1842, extract and fermented garlic, Allium sativum Linnaeus, 1753, supernatant, showed inhibitory activities against antibiotic-resistant A. hydrophila strains. Furthermore, the R. tomentosa extract and the fermented A. sativum supernatant exhibited significant antibacterial effects to several A. hydrophila strains, namely A. hydrophila CEDMA17.021, CEDMA17.002, CEDMA17.008, and CEDMA17.009 resistant to two or more antibiotics. This study demonstrated multiple resistant profiles of A. hydrophila strains to different antibiotics and the inhibitory activities of R. tomentosa extract and the fermented A. sativum supernatant against antibiotic-resistant A. hydrophila strains. Hence, this study indicates the potential use of bio-antibiotics derived from plants to manage Aeromonas-related infections.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46278680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-09DOI: 10.33997/j.afs.2022.35.2.003
S. Pattarapongpan, S. Arnupapboon, A. Arshad, T. Matsuishi
Whitespotted bambooshark, Chiloscyllium plagiosum (Anonymous [Bennett], 1830), is a major shark species caught in waters off Sabah, Malaysia. Despite the massive landing amount, its biological and stock status information is limited. In 2015–2016, the elasmobranch data collection was conducted in Southeast Asia, including Sabah. A yield-per-recruit (YPR) and spawning-per-recruit (SPR) analyses were performed to assess this data-deficient species’ stock status. The growth parameter, average maximum length, L∞, and growth rate, K, for males and females were 81.13 cm and 0.21 year-1 , and 84.30 cm and 0.18 year-1 , respectively. Limit and target biological reference points, maximum fishing mortality and fishing mortality corresponding to 10 % of YPR slope (Fmax and F0.1, respectively) for YPR, and fishing mortality corresponding to 20 % and 30 % of spawning stock remained (F20% and F30%, respectively) for SPR, were calculated. The results suggest neither growth nor recruit overfishing was occurring. Monitoring and surveillance of existing management measures are necessary to ensure sustainable utilisation of the stock.
{"title":"Stock Status of Whitespotted Bambooshark, Chiloscyllium plagiosum (Anonymous [Bennett], 1830) in Sabah, Malaysia, Using Yield-Per-Recruit and Spawning Per-Recruit Analyses","authors":"S. Pattarapongpan, S. Arnupapboon, A. Arshad, T. Matsuishi","doi":"10.33997/j.afs.2022.35.2.003","DOIUrl":"https://doi.org/10.33997/j.afs.2022.35.2.003","url":null,"abstract":"Whitespotted bambooshark, Chiloscyllium plagiosum (Anonymous [Bennett], 1830), is a major shark species caught in waters off Sabah, Malaysia. Despite the massive landing amount, its biological and stock status information is limited. In 2015–2016, the elasmobranch data collection was conducted in Southeast Asia, including Sabah. A yield-per-recruit (YPR) and spawning-per-recruit (SPR) analyses were performed to assess this data-deficient species’ stock status. The growth parameter, average maximum length, L∞, and growth rate, K, for males and females were 81.13 cm and 0.21 year-1 , and 84.30 cm and 0.18 year-1 , respectively. Limit and target biological reference points, maximum fishing mortality and fishing mortality corresponding to 10 % of YPR slope (Fmax and F0.1, respectively) for YPR, and fishing mortality corresponding to 20 % and 30 % of spawning stock remained (F20% and F30%, respectively) for SPR, were calculated. The results suggest neither growth nor recruit overfishing was occurring. Monitoring and surveillance of existing management measures are necessary to ensure sustainable utilisation of the stock.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46139716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-21DOI: 10.33997/j.afs.2022.35.2.002
Angel Queenee Daytic Dequito, V. Corre, Elfred John Abacan
Environmental factors influence the abundance of Vibrio species in shrimp culture systems. Prediction of the abundance of presumptive Vibrio species can help prevent the occurrence of bacterial diseases as this will provide insights about when and which environmental factors to manage. In this study, the parametric linear regression model (LRM) and negative binomial model (NBM), and semiparametric generalised additive model (GAM) were used to identify correlations and predict changes of Vibrio abundance with physicochemical and biological water parameters. Water parameters were recorded from three 300 m2 biofloc ponds stocked with Penaeus vannamei Boone, 1931, at 500 individuals.m-3 over four culture run periods. Each culture run lasted for 16 weeks. Imputed data were initially subjected to univariate analysis and Pearson’s correlation analysis. The abundance of presumptive Vibrio species was found to be highly correlated with alkalinity, pH, and phytoplankton density. GAM performed best among the three models based on Akaike’s information criterion (AIC), having the smallest value of 5,743.222 compared to 6,572.014 and 5,857.997 values for ordinary LRM and NBM, respectively. It also had the largest deviance explained statistic with 41.2 % of the deviance reduced by including the predictors compared with ordinary LRM and NBM with only 16.04 % and 14.5 % deviance reduced, respectively. GAM introduced flexibility that predicts the dependent variable better in terms of statistical significance than LRM and NBM. It is important to consider using a semiparametric modelling approach as a tool for aquaculture management.
{"title":"Generalised Additive Model Improves Estimates of Vibrio Species Abundance in Penaeus vannamei Boone, 1931 Biofloc Production System","authors":"Angel Queenee Daytic Dequito, V. Corre, Elfred John Abacan","doi":"10.33997/j.afs.2022.35.2.002","DOIUrl":"https://doi.org/10.33997/j.afs.2022.35.2.002","url":null,"abstract":"Environmental factors influence the abundance of Vibrio species in shrimp culture systems. Prediction of the abundance of presumptive Vibrio species can help prevent the occurrence of bacterial diseases as this will provide insights about when and which environmental factors to manage. In this study, the parametric linear regression model (LRM) and negative binomial model (NBM), and semiparametric generalised additive model (GAM) were used to identify correlations and predict changes of Vibrio abundance with physicochemical and biological water parameters. Water parameters were recorded from three 300 m2 biofloc ponds stocked with Penaeus vannamei Boone, 1931, at 500 individuals.m-3 over four culture run periods. Each culture run lasted for 16 weeks. Imputed data were initially subjected to univariate analysis and Pearson’s correlation analysis. The abundance of presumptive Vibrio species was found to be highly correlated with alkalinity, pH, and phytoplankton density. GAM performed best among the three models based on Akaike’s information criterion (AIC), having the smallest value of 5,743.222 compared to 6,572.014 and 5,857.997 values for ordinary LRM and NBM, respectively. It also had the largest deviance explained statistic with 41.2 % of the deviance reduced by including the predictors compared with ordinary LRM and NBM with only 16.04 % and 14.5 % deviance reduced, respectively. GAM introduced flexibility that predicts the dependent variable better in terms of statistical significance than LRM and NBM. It is important to consider using a semiparametric modelling approach as a tool for aquaculture management.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44850835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-20DOI: 10.33997/j.afs.2022.35.2.001
B. Hang, Duong Thuy Yen
Aeromonas hydrophila is a major pathogen causing septicemic disease and high mortality in cultured bighead catfish, Clarias macrocephalus Günther, 1864. Crossbreeding for enhanced disease resistance may help alleviate the infection. This study aimed to evaluate the crossbreeding effects of bighead catfish from three broodstock sources, including one domesticated strain (Can Tho - CT) and two wild strains (Ca Mau - CM and Hau Giang - HG), on the innate immune response. Eight diallel crosses (excluding HG × CT) were reared in tanks for 100 days to the juvenile stage for use in this study. Forty-five bighead catfish juveniles of similar sizes, 4.5–6.1 g, were sampled from each cross to evaluate the immune response and stress indicators. Thirty fish from each cross were challenged with A. hydrophila. The results showed that innate immune responses of domesticated CT strains were higher than wild strains (HG and CM). Higher values of white blood cells, phagocytic activity, and lysozyme activity were observed in CT × CT and CT × CM crosses. These crosses had significantly lower cortisol levels and lower mortality rates of 40 % (CT × CT) and 43.3 % (CT × CM) after being challenged with A. hydrophila compared to the other crosses. The results demonstrate that domesticated CT strain and crossbreeding between CT and CM wild strains could improve the innate immune system and resistance to A. hydrophila. The innate immune responses of the domesticated CT strain were higher than wild strains (HG and CM).
{"title":"Immune Status of Bighead Catfish (Clarias macrocephalus Günther, 1864) Crossbreeds Between Wild and Domesticated Strains and Their Response to Challenge With Aeromonas hydrophila","authors":"B. Hang, Duong Thuy Yen","doi":"10.33997/j.afs.2022.35.2.001","DOIUrl":"https://doi.org/10.33997/j.afs.2022.35.2.001","url":null,"abstract":"Aeromonas hydrophila is a major pathogen causing septicemic disease and high mortality in cultured bighead catfish, Clarias macrocephalus Günther, 1864. Crossbreeding for enhanced disease resistance may help alleviate the infection. This study aimed to evaluate the crossbreeding effects of bighead catfish from three broodstock sources, including one domesticated strain (Can Tho - CT) and two wild strains (Ca Mau - CM and Hau Giang - HG), on the innate immune response. Eight diallel crosses (excluding HG × CT) were reared in tanks for 100 days to the juvenile stage for use in this study. Forty-five bighead catfish juveniles of similar sizes, 4.5–6.1 g, were sampled from each cross to evaluate the immune response and stress indicators. Thirty fish from each cross were challenged with A. hydrophila. The results showed that innate immune responses of domesticated CT strains were higher than wild strains (HG and CM). Higher values of white blood cells, phagocytic activity, and lysozyme activity were observed in CT × CT and CT × CM crosses. These crosses had significantly lower cortisol levels and lower mortality rates of 40 % (CT × CT) and 43.3 % (CT × CM) after being challenged with A. hydrophila compared to the other crosses. The results demonstrate that domesticated CT strain and crossbreeding between CT and CM wild strains could improve the innate immune system and resistance to A. hydrophila. The innate immune responses of the domesticated CT strain were higher than wild strains (HG and CM).","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44680325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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