Chen Yu, Li Yiping, Zhu Ya, Guo Jinchuan, Wu Weixiong, Wei Yao, Wang Can, Shang Heqin, Wang Yu
{"title":"Spatial and temporal characteristics of mixed layer depth and anoxic zone during the extinction of thermal stratification in southern reservoirs in China—A case of Tianbao Reservoir, Nanning City","authors":"Chen Yu, Li Yiping, Zhu Ya, Guo Jinchuan, Wu Weixiong, Wei Yao, Wang Can, Shang Heqin, Wang Yu","doi":"10.18307/2023.0523","DOIUrl":"https://doi.org/10.18307/2023.0523","url":null,"abstract":"","PeriodicalId":37454,"journal":{"name":"Hupo Kexue/Journal of Lake Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135357795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wang Yao, Mao Changping, Jiang Zhimin, Wang Shuai, Rao Wenbo
{"title":"Distribution characteristics and controlling factors of reduced inorganic sulfur forms in Three Gorges Reservoir sediment and its influence on the release of endogenous phosphorus","authors":"Wang Yao, Mao Changping, Jiang Zhimin, Wang Shuai, Rao Wenbo","doi":"10.18307/2023.0207","DOIUrl":"https://doi.org/10.18307/2023.0207","url":null,"abstract":"","PeriodicalId":37454,"journal":{"name":"Hupo Kexue/Journal of Lake Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67483337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liao Yuanshan, Xiao Qitao, Liu Zhenjing, Hu Zhenghua, Zhang Mi, Xiao Wei, Duan Hongtao
{"title":"Nitrous oxide emission and its influencing factors at the cyanobacteria-dominated lake","authors":"Liao Yuanshan, Xiao Qitao, Liu Zhenjing, Hu Zhenghua, Zhang Mi, Xiao Wei, Duan Hongtao","doi":"10.18307/2023.0216","DOIUrl":"https://doi.org/10.18307/2023.0216","url":null,"abstract":"","PeriodicalId":37454,"journal":{"name":"Hupo Kexue/Journal of Lake Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67483442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Yuqing, Chen Xin, Lv Chaochao, Shan Hang, Chou Qingchuan, Lv Xingju, Zhang Xiaolin, Ni Leyi, Cao Te
{"title":"Diversity and distribution status of aquatic plants in the lakeshore zone of Lake Erhai","authors":"T. Yuqing, Chen Xin, Lv Chaochao, Shan Hang, Chou Qingchuan, Lv Xingju, Zhang Xiaolin, Ni Leyi, Cao Te","doi":"10.18307/2023.0314","DOIUrl":"https://doi.org/10.18307/2023.0314","url":null,"abstract":"","PeriodicalId":37454,"journal":{"name":"Hupo Kexue/Journal of Lake Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67484220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Temporal and spatial evolution characteristics of runoff in the Xijiang River Basin in recent 60 years under the changing environment","authors":"Lin Jiqing, Zhang Peng, Zhao Yingyu, Liu Yang","doi":"10.18307/2023.0322","DOIUrl":"https://doi.org/10.18307/2023.0322","url":null,"abstract":"","PeriodicalId":37454,"journal":{"name":"Hupo Kexue/Journal of Lake Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67484726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Chenhui, Yang Xingcheng, D. Jue, Chen Yuchen, Lin Yuqing, Chen Qiuwen, Pan Baozhu
{"title":"Distribution characteristics and controlling mechanisms of nitrogen and phosphorus in water under cascade dam construction of Lancangjiang River","authors":"L. Chenhui, Yang Xingcheng, D. Jue, Chen Yuchen, Lin Yuqing, Chen Qiuwen, Pan Baozhu","doi":"10.18307/2023.0424","DOIUrl":"https://doi.org/10.18307/2023.0424","url":null,"abstract":"","PeriodicalId":37454,"journal":{"name":"Hupo Kexue/Journal of Lake Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67486124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zheng Dayan, L. Rui, Liuliu Zhang, Caigui Zheng, Zhang Jing
{"title":"Effects of land use on multi-temporal scales of dissolved organic matter in Three Gorges Reservoir","authors":"Zheng Dayan, L. Rui, Liuliu Zhang, Caigui Zheng, Zhang Jing","doi":"10.18307/2023.0426","DOIUrl":"https://doi.org/10.18307/2023.0426","url":null,"abstract":"","PeriodicalId":37454,"journal":{"name":"Hupo Kexue/Journal of Lake Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67486274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wu Dongshao, Cao Min, Duan Zhongzhao, Zhang Yuan, Ghen Wei
{"title":"The variation and source-sink effect of total phosphorus flux input and output of Lake Dianchi during 2001-2018","authors":"Wu Dongshao, Cao Min, Duan Zhongzhao, Zhang Yuan, Ghen Wei","doi":"10.18307/2023.0423","DOIUrl":"https://doi.org/10.18307/2023.0423","url":null,"abstract":"","PeriodicalId":37454,"journal":{"name":"Hupo Kexue/Journal of Lake Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67486528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhou Chunhua, Wang Rongrong, W. Sheng, Guo Ting, Ou Shan, Wu Xiaoping
{"title":"Fish diversity in Nanchang section of the lower Ganjiang River based on environmental DNA metabarcoding","authors":"Zhou Chunhua, Wang Rongrong, W. Sheng, Guo Ting, Ou Shan, Wu Xiaoping","doi":"10.18307/2023.0435","DOIUrl":"https://doi.org/10.18307/2023.0435","url":null,"abstract":"","PeriodicalId":37454,"journal":{"name":"Hupo Kexue/Journal of Lake Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67486811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
噬藻体在蓝藻水华发生后期快速增殖被认为是蓝藻水华消亡的重要途径,但有关噬藻体的应用却鲜有报道。本研究以噬藻体A-4(L)侵染鱼腥藻PCC 7120为例,开展光照、温度和感染复数影响噬藻体裂解藻细胞的探究,推定噬藻体在裂解蓝藻应用中的最佳投放时间及投放剂量。结果显示,光照是影响噬藻体裂解藻细胞最关键的因子,A-4(L)在全光照条件下且感染复数为0.01时,8 h即可快速裂解鱼腥藻PCC 7120,但在全黑暗条件下无裂解;光照时长越长,A-4(L)对藻细胞的裂解越快。进一步研究发现,A-4(L)在藻细胞表面的吸附不依赖光照,但在黑暗条件下A-4(L)在藻细胞内的复制受到抑制,推测A-4(L)的复制可能与宿主的光合作用有关。在15~25℃范围内,提高温度会加快A-4(L)裂解藻细胞的速度,且胞外A-4(L)效价随着温度升高而增加。在10-6~1范围内,感染复数每提高两个数量级,A-4(L)裂解藻细胞则提前4 h。综合上述结果,在7:00向湖水样品中投加感染复数为0.01的噬藻体可使PCC 7120藻细胞生物量在24 h内大幅度降低76%。本研究为蓝藻水华发生时投加噬藻体控制蓝藻种群密度提供一定基础。;Cyanophage can proliferate rapidly in the late stages of cyanobacterial blooms. Cyanophage lysis of cyanobacteria has been considered an important pathway that can induce cyanobacterial apoptosis. However, few reports have focused on the use of cyanophages. In this study, the infection of Anabaena sp. PCC 7120 by cyanophage A-4(L) was used as an example to investigate the influence of illumination, temperature and multiplicity of infection on cyanophage lysis, which can help us to determine the best timing and concentration of cyanophage addition. Our results showed that illumination was a key factor affecting cell lysis. A-4(L) lysed Anabaena sp. PCC 7120 after 8 hours at a MOI of 0.01 during continuous illumination, whereas no lysis occurred during dark incubation. In addition, prolonged illumination resulted in more rapid infection. Further studies showed that adsorption was not dependent on illumination. However, intracellular replication of cyanophage DNA was inhibited under dark conditions. It is speculated that the replication of A-4(L) DNA may be related to the photosynthesis of the host. Within the range of 15-25℃, higher temperature improved the lysis rate. The extracellular A-4(L) titer also increased with higher temperature. In the range of 10-6-1, A-4(L) lysed cells 4 h earlier when the MOI increased by two orders of magnitude. Taken together, we proposed the addition of cyanophage at a MOI of 0.01 between 7:00 and 12:00 for the purpose of bloom treatment. Further field experiments showed that the addition of cyanophage at a MOI of 0.01 at 7:00 am did indeed significantly reduce the biomass of PCC7120 algal cells by 76% within 24 hours. This study provides a basis for using cyanophages to control the abundance of cyanobacteria during cyanobacterial blooms.
噬藻体在蓝藻水华发生后期快速增殖被认为是蓝藻水华消亡的重要途径,但有关噬藻体的应用却鲜有报道。本研究以噬藻体A-4(L)侵染鱼腥藻PCC 7120为例,开展光照、温度和感染复数影响噬藻体裂解藻细胞的探究,推定噬藻体在裂解蓝藻应用中的最佳投放时间及投放剂量。结果显示,光照是影响噬藻体裂解藻细胞最关键的因子,A-4(L)在全光照条件下且感染复数为0.01时,8 h即可快速裂解鱼腥藻PCC 7120,但在全黑暗条件下无裂解;光照时长越长,A-4(L)对藻细胞的裂解越快。进一步研究发现,A-4(L)在藻细胞表面的吸附不依赖光照,但在黑暗条件下A-4(L)在藻细胞内的复制受到抑制,推测A-4(L)的复制可能与宿主的光合作用有关。在15~25℃范围内,提高温度会加快A-4(L)裂解藻细胞的速度,且胞外A-4(L)效价随着温度升高而增加。在10-6~1范围内,感染复数每提高两个数量级,A-4(L)裂解藻细胞则提前4 h。综合上述结果,在7:00向湖水样品中投加感染复数为0.01的噬藻体可使PCC 7120藻细胞生物量在24 h内大幅度降低76%。本研究为蓝藻水华发生时投加噬藻体控制蓝藻种群密度提供一定基础。;Cyanophage can proliferate rapidly in the late stages of cyanobacterial blooms. Cyanophage lysis of cyanobacteria has been considered an important pathway that can induce cyanobacterial apoptosis. However, few reports have focused on the use of cyanophages. In this study, the infection of Anabaena sp. PCC 7120 by cyanophage A-4(L) was used as an example to investigate the influence of illumination, temperature and multiplicity of infection on cyanophage lysis, which can help us to determine the best timing and concentration of cyanophage addition. Our results showed that illumination was a key factor affecting cell lysis. A-4(L) lysed Anabaena sp. PCC 7120 after 8 hours at a MOI of 0.01 during continuous illumination, whereas no lysis occurred during dark incubation. In addition, prolonged illumination resulted in more rapid infection. Further studies showed that adsorption was not dependent on illumination. However, intracellular replication of cyanophage DNA was inhibited under dark conditions. It is speculated that the replication of A-4(L) DNA may be related to the photosynthesis of the host. Within the range of 15-25℃, higher temperature improved the lysis rate. The extracellular A-4(L) titer also increased with higher temperature. In the range of 10-6-1, A-4(L) lysed cells 4 h earlier when the MOI increased by two orders of magnitude. Taken together, we proposed the addition of cyanophage at a MOI of 0.01 between 7:00 and 12:00 for the purpose of bloom treatment. Further field experiments showed that the addition of cyanophage at a MOI of 0.01 at 7:00 am did indeed significantly reduce the biomass of PCC7120 algal cells by 76% within 24 hours. This study provides a basis for using cyanophages to control the abundance of cyanobacteria during cyanobacterial blooms.
{"title":"Appling cyanophage to lyse cyanobacterium—Taking the infection of <i>Anabaena</i> sp. PCC 7120 by cyanophage A-4 (L) as an example","authors":"Cheng Ting, Yang Yiling, Li Qi, Gan Nanqin","doi":"10.18307/2023.0516","DOIUrl":"https://doi.org/10.18307/2023.0516","url":null,"abstract":"噬藻体在蓝藻水华发生后期快速增殖被认为是蓝藻水华消亡的重要途径,但有关噬藻体的应用却鲜有报道。本研究以噬藻体A-4(L)侵染鱼腥藻PCC 7120为例,开展光照、温度和感染复数影响噬藻体裂解藻细胞的探究,推定噬藻体在裂解蓝藻应用中的最佳投放时间及投放剂量。结果显示,光照是影响噬藻体裂解藻细胞最关键的因子,A-4(L)在全光照条件下且感染复数为0.01时,8 h即可快速裂解鱼腥藻PCC 7120,但在全黑暗条件下无裂解;光照时长越长,A-4(L)对藻细胞的裂解越快。进一步研究发现,A-4(L)在藻细胞表面的吸附不依赖光照,但在黑暗条件下A-4(L)在藻细胞内的复制受到抑制,推测A-4(L)的复制可能与宿主的光合作用有关。在15~25℃范围内,提高温度会加快A-4(L)裂解藻细胞的速度,且胞外A-4(L)效价随着温度升高而增加。在10<sup>-6</sup>~1范围内,感染复数每提高两个数量级,A-4(L)裂解藻细胞则提前4 h。综合上述结果,在7:00向湖水样品中投加感染复数为0.01的噬藻体可使PCC 7120藻细胞生物量在24 h内大幅度降低76%。本研究为蓝藻水华发生时投加噬藻体控制蓝藻种群密度提供一定基础。;Cyanophage can proliferate rapidly in the late stages of cyanobacterial blooms. Cyanophage lysis of cyanobacteria has been considered an important pathway that can induce cyanobacterial apoptosis. However, few reports have focused on the use of cyanophages. In this study, the infection of Anabaena sp. PCC 7120 by cyanophage A-4(L) was used as an example to investigate the influence of illumination, temperature and multiplicity of infection on cyanophage lysis, which can help us to determine the best timing and concentration of cyanophage addition. Our results showed that illumination was a key factor affecting cell lysis. A-4(L) lysed Anabaena sp. PCC 7120 after 8 hours at a MOI of 0.01 during continuous illumination, whereas no lysis occurred during dark incubation. In addition, prolonged illumination resulted in more rapid infection. Further studies showed that adsorption was not dependent on illumination. However, intracellular replication of cyanophage DNA was inhibited under dark conditions. It is speculated that the replication of A-4(L) DNA may be related to the photosynthesis of the host. Within the range of 15-25℃, higher temperature improved the lysis rate. The extracellular A-4(L) titer also increased with higher temperature. In the range of 10<sup>-6</sup>-1, A-4(L) lysed cells 4 h earlier when the MOI increased by two orders of magnitude. Taken together, we proposed the addition of cyanophage at a MOI of 0.01 between 7:00 and 12:00 for the purpose of bloom treatment. Further field experiments showed that the addition of cyanophage at a MOI of 0.01 at 7:00 am did indeed significantly reduce the biomass of PCC7120 algal cells by 76% within 24 hours. This study provides a basis for using cyanophages to control the abundance of cyanobacteria during cyanobacterial blooms.","PeriodicalId":37454,"journal":{"name":"Hupo Kexue/Journal of Lake Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135358828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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