Pub Date : 2016-08-01DOI: 10.1016/j.aggene.2016.05.003
María Úbeda-Manzanaro, Juan B. Ortiz-Delgado, Carmen Sarasquete
Tert gene encodes a catalytic subunit of the enzyme telomerase, which protects telomeres from abnormal folds and their degradation. In mammals, the activity of telomerase in tissues of adults is limited to stem cells with high potential for proliferation, finding expression in the cells of the germline, tumors and neoplastic cells, although the Tert gene seems to be ubiquitous in fish. To gain insight on Tert implication for fish gonad cell differentiation and gametogenesis progress, we cloned the Tert cDNA of two reared marine fish species, gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax), and their quantitative and qualitative Tert mRNA expression were analyzed. Two Tert transcripts encoding proteins which differ at their functional C-terminal end were isolated from gilthead seabream, whereas only one Tert transcript was identified from European seabass. The qPCR assays showed that Tert genes are expressed ubiquitously in both fish species, and the highest expression levels were found in gonads and particularly in differentiating or maturating germ cells, which could suggest an important role of Tert genes in gametogenesis and cell-tissue development of fish species.
{"title":"Cloning and sequencing of Tert gene in gilthead seabream, Sparus aurata, and European seabass, Dicentrarchus labrax: Expression patterns in germ and somatic cells","authors":"María Úbeda-Manzanaro, Juan B. Ortiz-Delgado, Carmen Sarasquete","doi":"10.1016/j.aggene.2016.05.003","DOIUrl":"10.1016/j.aggene.2016.05.003","url":null,"abstract":"<div><p><em>Tert</em><span> gene encodes a catalytic subunit<span><span> of the enzyme telomerase, which protects telomeres from abnormal folds and their degradation. In mammals, the activity of telomerase in tissues of adults is limited to </span>stem cells with high potential for proliferation, finding expression in the cells of the germline, tumors and neoplastic cells, although the </span></span><em>Tert</em> gene seems to be ubiquitous in fish. To gain insight on <em>Tert</em><span><span> implication for fish gonad </span>cell differentiation<span> and gametogenesis progress, we cloned the </span></span><em>Tert</em><span> cDNA of two reared marine fish species, gilthead seabream (</span><span><em>Sparus aurata</em></span>) and European seabass (<span><em>Dicentrarchus labrax</em></span>), and their quantitative and qualitative <em>Tert</em> mRNA expression were analyzed. Two <em>Tert</em><span> transcripts encoding proteins which differ at their functional C-terminal end were isolated from gilthead seabream, whereas only one </span><em>Tert</em><span> transcript was identified from European seabass. The qPCR assays showed that </span><em>Tert</em> genes are expressed ubiquitously in both fish species, and the highest expression levels were found in gonads and particularly in differentiating or maturating germ cells, which could suggest an important role of <em>Tert</em> genes in gametogenesis and cell-tissue development of fish species.</p></div>","PeriodicalId":37751,"journal":{"name":"Agri Gene","volume":"1 ","pages":"Pages 23-32"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aggene.2016.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53993471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-08-01DOI: 10.1016/j.aggene.2016.05.001
Harshata Pal , Murali Sharaff , Avinash Sethi , Pranab Hazra , Debasis Mazumder , Shree P. Pandey
To decipher the effect of spontaneous monogenic mutations on fruit ripening associated gene expression pattern, an integrated effort has been made to investigate tomato colored mutants high pigment-1(hp-1), high pigment-2darkgreen (hp-2dg), old gold crimson(og)c and ripening impaired mutant ripening inhibitor (rin), during fruit ripening. Quantitative real time polymerase chain reaction was performed to assess relative transcript accumulation for carotenogenic pathway genes, ethylene anabolic genes, cell wall modifying genes and MADS-BOX transcription factor gene LeMADS-RIN. The non photomorphogenic high pigment mutant ogc was studied at the transcriptional level for the first time which provided the basis to select the mutant ogc as a suitable candidate of non-transgenic source for pigment enrichment by future development of hybrids. Regression analysis found that LeZDS and CYC-B were the sole positive contributors for lycopene and β-carotene content, respectively, and fruit firmness was negatively correlated to LeEXP, LePG, LeTBG-4, PME. Highly similar fruit firmness was observed in hp-2dg with the mutant rin, which is desirable for extended shelf life. Expression of the major ripening regulator gene LeMADS-RIN was also induced in high pigment mutants. Future characterization of the promoter region of candidate genes may decipher unknown regulatory aspects of these mutants.
{"title":"Deciphering the effect of mutations on fruit ripening quality associated gene expression pattern in spontaneous monogenic tomato mutants","authors":"Harshata Pal , Murali Sharaff , Avinash Sethi , Pranab Hazra , Debasis Mazumder , Shree P. Pandey","doi":"10.1016/j.aggene.2016.05.001","DOIUrl":"10.1016/j.aggene.2016.05.001","url":null,"abstract":"<div><p><span>To decipher the effect of spontaneous monogenic mutations on fruit ripening associated gene expression pattern, an integrated effort has been made to investigate tomato colored mutants </span><em>high pigment-1</em>(<em>hp-1</em>), <em>high pigment-2</em><sup><em>dark</em></sup><em>green</em> (<em>hp-2</em><sup><em>dg</em></sup>), <em>old gold crimson</em>(<em>og</em>)<sup><em>c</em></sup> and ripening impaired mutant <em>ripening inhibitor</em> (<em>rin</em><span><span>), during fruit ripening. Quantitative real time polymerase chain reaction was performed to assess relative transcript accumulation for carotenogenic pathway genes, ethylene anabolic genes, cell wall </span>modifying genes and MADS-BOX transcription factor gene </span><em>LeMADS-RIN</em>. The non photomorphogenic high pigment mutant <em>og</em><sup><em>c</em></sup> was studied at the transcriptional level for the first time which provided the basis to select the mutant <em>og</em><sup><em>c</em></sup> as a suitable candidate of non-transgenic source for pigment enrichment by future development of hybrids. Regression analysis found that <em>LeZDS</em> and <em>CYC-B</em><span> were the sole positive contributors for lycopene and β-carotene content, respectively, and fruit firmness was negatively correlated to </span><em>LeEXP</em>, <em>LePG</em>, <em>LeTBG-4</em>, <em>PME</em>. Highly similar fruit firmness was observed in <em>hp-2</em><sup><em>dg</em></sup> with the mutant <em>rin</em><span>, which is desirable for extended shelf life. Expression of the major ripening regulator gene </span><em>LeMADS-RIN</em><span> was also induced in high pigment mutants. Future characterization of the promoter region of candidate genes may decipher unknown regulatory aspects of these mutants.</span></p></div>","PeriodicalId":37751,"journal":{"name":"Agri Gene","volume":"1 ","pages":"Pages 1-14"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aggene.2016.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53993753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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