Pub Date : 2019-09-01DOI: 10.1016/j.wndm.2019.100162
R. Rathinamoorthy , L. Sasikala
The research aimed to develop a bioactive wound dressing using chitosan and Leptospermum scoparium honey, which is commonly known as Manuka Honey. The wound dressing was developed using solvent- casting technique with the honey as a drug. The wound healing ability of the developed dressing was evaluated against three different wound models namely excision, incision and burn wound model in terms of wound contraction percentage and histopathological examination. The results of the study revealed that the performance of the developed honey loaded chitosan dressing was comparable with commercial dressing. In the case of excision and incision wound models, complete healing was observed in on 18th day and in the case of burn wound model on 21 st day. The wound contraction percentage of CH dressed excision wound model is noted significantly better (0.05> p; 94%) than the commercial dressing (90%). In incision wound model, the tensile strength of healing skin was noted significantly higher (0.05> p) for CH dressed wounds than the commercial dressing. Similarly, for the burn wound also a significantly improved performance was noted it is noted (0.05> p; 96%) for CH dressing than the commercial dressing (85%). The results of the study indicated that the CH dressing have higher potential for the wound healing application.
{"title":"In vivo – Wound healing studies of Leptospermum scoparium honey loaded chitosan bioactive wound dressing","authors":"R. Rathinamoorthy , L. Sasikala","doi":"10.1016/j.wndm.2019.100162","DOIUrl":"10.1016/j.wndm.2019.100162","url":null,"abstract":"<div><p><span>The research aimed to develop a bioactive wound dressing using chitosan and </span><em>Leptospermum scoparium</em><span><span><span> honey, which is commonly known as Manuka Honey. The wound dressing was developed using solvent- casting technique with the honey as a drug. The wound healing ability of the developed dressing was evaluated against three different wound models namely excision, </span>incision and burn wound model in terms of </span>wound contraction<span> percentage and histopathological examination. The results of the study revealed that the performance of the developed honey loaded chitosan dressing was comparable with commercial dressing. In the case of excision and incision wound models, complete healing was observed in on 18th day and in the case of burn wound model on 21 st day. The wound contraction percentage of CH dressed excision wound model is noted significantly better (0.05> p; 94%) than the commercial dressing (90%). In incision wound model, the tensile strength of healing skin was noted significantly higher (0.05> p) for CH dressed wounds than the commercial dressing. Similarly, for the burn wound also a significantly improved performance was noted it is noted (0.05> p; 96%) for CH dressing than the commercial dressing (85%). The results of the study indicated that the CH dressing have higher potential for the wound healing application.</span></span></p></div>","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"26 1","pages":"Article 100162"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44701529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-01DOI: 10.1016/j.wndm.2019.100161
Ana Luisa Muñoz , William Hernando Merchán , Ana Luiza Resende Pires , Ângela Maria Moraes , Lina Andrea Gómez
Venous ulcers are a common disease caused by circulatory insufficiency, having as characteristics slow healing and therefore a difficult treatment. Consequently, this disease has a high impact at personal, professional and social levels, representing large costs for both patients and healthcare system. The aim of the present pilot study was to evaluate the effectiveness of an alternative treatment for this specific type of wound, based on the use of autologous platelet-rich plasma gel (PRP) combined with biocompatible porous membranes produced with the polysaccharides chitosan (C) and alginate (A). The applicability, security and healing efficacy of this treatment was evaluated in four patients suffering from venous ulcers with an at least 6-month of evolution that did not heal after conventional treatments. The clinical evolution analysis during the treatment was performed by periodic measurement of the lesions area for an average of 5.4 months. The results attained showed that the use of this treatment led to completed reduction in the ulcers area as well as to pain decrease. Hence, the combination between autologous PRP gel and chitosan-alginate (CA) porous membranes could be a useful alternative to treat chronic skin ulcers in humans.
{"title":"Biostimulation of venous chronic ulcers with platelet-rich plasma gel and biocompatible membranes of chitosan and alginate: A pilot study","authors":"Ana Luisa Muñoz , William Hernando Merchán , Ana Luiza Resende Pires , Ângela Maria Moraes , Lina Andrea Gómez","doi":"10.1016/j.wndm.2019.100161","DOIUrl":"10.1016/j.wndm.2019.100161","url":null,"abstract":"<div><p>Venous ulcers are a common disease caused by circulatory insufficiency, having as characteristics slow healing and therefore a difficult treatment. Consequently, this disease has a high impact at personal, professional and social levels, representing large costs for both patients and healthcare system. The aim of the present pilot study was to evaluate the effectiveness of an alternative treatment for this specific type of wound, based on the use of autologous platelet-rich plasma gel (PRP) combined with biocompatible porous membranes produced with the polysaccharides chitosan (C) and alginate (A). The applicability, security and healing efficacy of this treatment was evaluated in four patients suffering from venous ulcers with an at least 6-month of evolution that did not heal after conventional treatments. The clinical evolution analysis during the treatment was performed by periodic measurement of the lesions area for an average of 5.4 months. The results attained showed that the use of this treatment led to completed reduction in the ulcers area as well as to pain decrease. Hence, the combination between autologous PRP gel and chitosan-alginate (CA) porous membranes could be a useful alternative to treat chronic skin ulcers in humans.</p></div>","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"26 1","pages":"Article 100161"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41871528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetic foot ulcer is a serious complication of diabetes, resulting from peripheral neuropathy, macrovascular and microvascular disease, impaired angiogenesis and chronic inflammation. Epiphyllum oxypetalum leaves have been proven to be efficacious in incision wound healing. This project aimed to evaluate the wound healing properties of topical application of Epiphyllum oxypetalum leaves in diabetic mice. Diabetes was induced using streptozotocin. Wound healing activity was assessed by incision model. A total of 40 mice were used in this project, divided into four groups: WE10 and WE20 groups were treated topically with 10% and 20% Epiphyllum oxypetalum leaf extract ointment, respectively, while the diabetic control and non-diabetic groups were treated topically with the ointment base. The results showed significant wound healing progress in mice treated with Epiphyllum oxypetalum extract ointment, as assessed by wound contraction, number of macrophages and fibroblast count. In conclusion, topical application of 96% ethanol extract of Epiphyllum oxypetalum leaves could accelerate the wound healing time in diabetic mice, where the best activity was shown by 20% Epiphyllum oxypetalum extract.
{"title":"Wound healing properties of Epiphyllum oxypetalum (DC.) Haw. leaf extract in streptozotocin-induced diabetic mice by topical application","authors":"Lusi Putri Dwita, Faridlatul Hasanah, Ryana Srirustami, Repi, Riyan Purnomo, Sri Harsodjo","doi":"10.1016/j.wndm.2019.100160","DOIUrl":"10.1016/j.wndm.2019.100160","url":null,"abstract":"<div><p>Diabetic foot ulcer is a serious complication of diabetes, resulting from peripheral neuropathy, macrovascular and microvascular disease, impaired angiogenesis and chronic inflammation. <em>Epiphyllum oxypetalum</em> leaves have been proven to be efficacious in incision wound healing. This project aimed to evaluate the wound healing properties of topical application of <em>Epiphyllum oxypetalum</em> leaves in diabetic mice. Diabetes was induced using streptozotocin. Wound healing activity was assessed by incision model. A total of 40 mice were used in this project, divided into four groups: WE10 and WE20 groups were treated topically with 10% and 20% <em>Epiphyllum oxypetalum</em> leaf extract ointment, respectively, while the diabetic control and non-diabetic groups were treated topically with the ointment base. The results showed significant wound healing progress in mice treated with <em>Epiphyllum oxypetalum</em> extract ointment, as assessed by wound contraction, number of macrophages and fibroblast count. In conclusion, topical application of 96% ethanol extract of <em>Epiphyllum oxypetalum</em> leaves could accelerate the wound healing time in diabetic mice, where the best activity was shown by 20% <em>Epiphyllum oxypetalum</em> extract.</p></div>","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"26 1","pages":"Article 100160"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48350124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-01DOI: 10.1016/j.wndm.2019.100153
Michael Schmitz
Aim
Negative pressure wound therapy (NPWT) has become an accepted treatment modality for acute and chronic wounds with accelerated healing rates observed. To prevent air from being sucked in from the external environment, the wound and the filler that rests inside or upon the wound are hermetically sealed with an airtight adhesive polyurethane drape that is permeable to water vapour, transparent, and bacteria proof [1]. During the removal of the dressing skin stress occurs. An essential role is shearing force between skin and wound dressing playing [7]. This problem includes missing elasticity, flexibility and to strong adhesion [7,8]. The present study was initiated to evaluate if the switchable formulation of the adhesive ensures a durable and effective occlusion of the dressing and function of the NPWT, but also that the defined adhesion is in the range of patient- and user satisfaction.
Methods
The used film dressing consists of a thin film backing with a non‑latex adhesive. Before removal the adhesion is reduced by illuminating the dressing with the UV-A lamp.
Results
50 patients at a mean age of 71.7 years (SD ± 12.0) with a total of 51 wounds (one patient had two wounds) were included in the study. Wound bed condition (multiple answers possible) was rated as granulation (47), sloughy (33) and necrotic tissue (3). Wound bed condition had improved during the short course of the study with a reduction of sloughy and necrotic tissue.
Wound pain/adjacent to the wound pain was specified with a mean of 3,44 (SD ± 1,4) on the VAS (0 = no pain, 10 = excruciating pain) and was decreasing to a mean of 2,3 (SD ± 1,2) at the final visit (see Table 2 and Fig. 2). The application of the dressing/occlusion with the dressing was rated in mean with 1,02 (1. Visit) and 1,00 (2. Visit) on the 6–point-scale (1 = very good – 6 = insufficient). Significance was calculated as 0,000 for visit 1 (one sample t-test [test value=3,0]). Reactions on the wound edge/wound surroundings have not been reported. In contrast, a reduction of maceration, redness and dryness of the wound edge/wound surrounding was observed (see Table 1).
Discussion
The clinicians gave favourable scores for the tested film dressing regarding application of the dressing, application of NPWT/connection with the suction, overall satisfaction with the application, tightness of the dressing since the last dressing change, easiness of illuminating the dressing with the UV-A lamp and removal of the dressing after illumination with the UV-A lamp. The overall satisfaction with the product was rated very good. Tolerability such as pain during wearing the dressing and upon removal were given good scores. Existing wound edge and wound surroundings findings like maceration, redness etc. at visit 1 decreased. Skin irritation
{"title":"Pilot-study switchable film dressing & NPWT: A non-interventional, non-placebo-controlled, national pilot study","authors":"Michael Schmitz","doi":"10.1016/j.wndm.2019.100153","DOIUrl":"10.1016/j.wndm.2019.100153","url":null,"abstract":"<div><h3>Aim</h3><p>Negative pressure wound therapy (NPWT) has become an accepted treatment modality for acute and chronic wounds with accelerated healing rates observed. To prevent air from being sucked in from the external environment, the wound and the filler that rests inside or upon the wound are hermetically sealed with an airtight adhesive polyurethane drape that is permeable to water vapour, transparent, and bacteria proof [<span>1</span>]. During the removal of the dressing skin stress occurs. An essential role is shearing force between skin and wound dressing playing [<span>7</span>]. This problem includes missing elasticity, flexibility and to strong adhesion [<span>7</span>,<span>8</span>]. The present study was initiated to evaluate if the switchable formulation of the adhesive ensures a durable and effective occlusion of the dressing and function of the NPWT, but also that the defined adhesion is in the range of patient- and user satisfaction.</p></div><div><h3>Methods</h3><p>The used film dressing consists of a thin film backing with a non‑latex adhesive. Before removal the adhesion is reduced by illuminating the dressing with the UV-A lamp.</p></div><div><h3>Results</h3><p>50 patients at a mean age of 71.7 years (SD ± 12.0) with a total of 51 wounds (one patient had two wounds) were included in the study. Wound bed condition (multiple answers possible) was rated as granulation (47), sloughy (33) and necrotic tissue (3). Wound bed condition had improved during the short course of the study with a reduction of sloughy and necrotic tissue.</p><p>Wound pain/adjacent to the wound pain was specified with a mean of 3,44 (SD ± 1,4) on the VAS (0 = no pain, 10 = excruciating pain) and was decreasing to a mean of 2,3 (SD ± 1,2) at the final visit (see <span>Table 2</span> and <span>Fig. 2</span>). The application of the dressing/occlusion with the dressing was rated in mean with 1,02 (1. Visit) and 1,00 (2. Visit) on the 6–point-scale (1 = very good – 6 = insufficient). Significance was calculated as 0,000 for visit 1 (one sample t-test [test value=3,0]). Reactions on the wound edge/wound surroundings have not been reported. In contrast, a reduction of maceration, redness and dryness of the wound edge/wound surrounding was observed (see <span>Table 1</span>).</p></div><div><h3>Discussion</h3><p>The clinicians gave favourable scores for the tested film dressing regarding application of the dressing, application of NPWT/connection with the suction, overall satisfaction with the application, tightness of the dressing since the last dressing change, easiness of illuminating the dressing with the UV-A lamp and removal of the dressing after illumination with the UV-A lamp. The overall satisfaction with the product was rated very good. Tolerability such as pain during wearing the dressing and upon removal were given good scores. Existing wound edge and wound surroundings findings like maceration, redness etc. at visit 1 decreased. Skin irritation ","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"26 1","pages":"Article 100153"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43668737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stryphnodendron adstringens,commonly known as “barbatimão”, is a Brazilian savannah plant that has been long used in folk medicine due to its wound healing properties. However, there is a lack of integrated analyses of its healing activity based on scientific evidence.
Methods
The present study provides a literature review based on articles published in journals indexed in PUBMED and SCIELO databases. Moreover, a case series of four patients treated with a commercial barbatimão bark extract ointment is described.
Results and discussion
Forty-two publications on barbatimão were included in this review, most of which were published in English. From these studies, several biological and clinical actions of barbatimão were identified including anti-inflammatory, antioxidant, anti-ulcer, antimicrobial, and anti-parasitic effects, in addition to healing properties. The healing ability of barbatimão is scientifically described using evidence mainly obtained from animal experimental studies, and only one clinical trial exists that describes its healing effect on decubitus ulcers. Therefore, it was considered relevant to report a case series of patients subjected to barbatimão treatment for wounds caused by intense involuntary physical trauma (external accidents) or surgery. Considering all cases, the complete wound healing duration was 3.6 ± 0.8 months. Barbatimão is a tannin-rich plant, and chemical matrix analysis suggests that its wound healing effect is mainly due to high levels of epigallocatechin-gallate (EGCG) and gallic acid molecules.
Conclusion
Considering the difficulty in treating chronic or extensive wounds, barbatimão could be an effective, low-cost phytotherapy formulation with low levels of toxicity.
{"title":"Healing activity of Stryphnodendron adstringens (Mart.), a Brazilian tannin-rich species: A review of the literature and a case series","authors":"Neida Luiza Pellenz , Fernanda Barbisan , Veronica Farina Azzolin , Liana Pinheiro Santos Marques , Moisés Henrique Mastella , Cibele Ferreira Teixeira , Euler Esteves Ribeiro , Ivana Beatrice Mânica da Cruz","doi":"10.1016/j.wndm.2019.100163","DOIUrl":"10.1016/j.wndm.2019.100163","url":null,"abstract":"<div><h3>Introduction</h3><p><em>Stryphnodendron adstringens,</em>commonly known as “barbatimão”, is a Brazilian savannah plant that has been long used in folk medicine due to its wound healing properties. However, there is a lack of integrated analyses of its healing activity based on scientific evidence.</p></div><div><h3>Methods</h3><p>The present study provides a literature review based on articles published in journals indexed in PUBMED and SCIELO databases. Moreover, a case series of four patients treated with a commercial barbatimão bark extract ointment is described.</p></div><div><h3>Results and discussion</h3><p>Forty-two publications on barbatimão were included in this review, most of which were published in English. From these studies, several biological and clinical actions of barbatimão were identified including anti-inflammatory, antioxidant, anti-ulcer, antimicrobial, and anti-parasitic effects, in addition to healing properties. The healing ability of barbatimão is scientifically described using evidence mainly obtained from animal experimental studies, and only one clinical trial exists that describes its healing effect on decubitus ulcers. Therefore, it was considered relevant to report a case series of patients subjected to barbatimão treatment for wounds caused by intense involuntary physical trauma (external accidents) or surgery. Considering all cases, the complete wound healing duration was 3.6 ± 0.8 months. Barbatimão is a tannin-rich plant, and chemical matrix analysis suggests that its wound healing effect is mainly due to high levels of epigallocatechin-gallate (EGCG) and gallic acid molecules.</p></div><div><h3>Conclusion</h3><p>Considering the difficulty in treating chronic or extensive wounds, barbatimão could be an effective, low-cost phytotherapy formulation with low levels of toxicity.</p></div>","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"26 1","pages":"Article 100163"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41478355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Mitomycin-C (MMC) has been used for treating stricture. Topical MMC has been proven to inhibit fibroblasts proliferation and reduce the number of fibroblasts. Myofibroblasts are originally from fibroblasts. The scarring potential of myofibroblasts has been documented in many anatomical sites. The purpose of this study is to analyze the effect of topical MMC on myofibroblasts in healing anoplasty wounds in Wistar rats.
Methods
: An anoplasty procedure was performed on Wistar rats (n=30) that were subsequently randomly placed into two equal groups; i.e., control and treated groups. In the treated group, topical mitomycin-C was applied to the wound following the anoplasty procedure. Five rats in each group were weekly sacrificed. The myofibroblasts in tissue samples were identified by immunohistochemical analysis of alpha-smooth muscle actin. The total of myofibroblasts was evaluated by scoring methods. The Mann-Whitney test was used to analyze the statistical significance of differences, and p values below 0.05 were considered to be significant.
Results
: The median myofibroblasts scores in the first week for the treated and control groups were 5 and 9, respectively. In the second and third week, the median myofibroblasts scores were 6 for both groups. Statistical evaluations revealed significant differences between the groups only in the first week (p=0.007).
Conclusions
: Topical MMC reduced the myofibroblasts in the first week of healing anoplasty wounds in Wistar rats.
{"title":"Topical mitomycin-C reduced number of myofibroblasts in healing anoplasty wounds in Wistar rats","authors":"Harsali Fransiscus Lampus , Suryani As’ad , Rahmawati Minhajat , Warsinggih , Mochammad Hatta , Rina Masadah , Akhmad Makhmudi , Rosdiana Natzir , Wardihan Sinrang , Fachruddin Benyamin , Agussalim Bukhari , Nathania Wonoputri , Nita Mariana , Tommy Rubiyanto Habar","doi":"10.1016/j.wndm.2019.100154","DOIUrl":"10.1016/j.wndm.2019.100154","url":null,"abstract":"<div><h3>Background</h3><p><span>: Mitomycin-C (MMC) has been used for treating stricture. Topical MMC has been proven to inhibit fibroblasts proliferation and reduce the number of fibroblasts. Myofibroblasts are originally from fibroblasts. The scarring potential of myofibroblasts has been documented in many anatomical sites. The purpose of this study is to analyze the effect of topical MMC on myofibroblasts in healing </span>anoplasty wounds in Wistar rats.</p></div><div><h3>Methods</h3><p>: An anoplasty procedure was performed on Wistar rats (n=30) that were subsequently randomly placed into two equal groups; i.e., control and treated groups. In the treated group, topical mitomycin-C was applied to the wound following the anoplasty procedure. Five rats in each group were weekly sacrificed. The myofibroblasts in tissue samples were identified by immunohistochemical analysis of alpha-smooth muscle actin. The total of myofibroblasts was evaluated by scoring methods. The Mann-Whitney test was used to analyze the statistical significance of differences, and p values below 0.05 were considered to be significant.</p></div><div><h3>Results</h3><p>: The median myofibroblasts scores in the first week for the treated and control groups were 5 and 9, respectively. In the second and third week, the median myofibroblasts scores were 6 for both groups. Statistical evaluations revealed significant differences between the groups only in the first week (p=0.007).</p></div><div><h3>Conclusions</h3><p>: Topical MMC reduced the myofibroblasts in the first week of healing anoplasty wounds in Wistar rats.</p></div>","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"25 1","pages":"Article 100154"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100154","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45206388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.1016/j.wndm.2019.100151
Getachew Ayal , Assefa Belay , Wubayehu Kahaliw
Ethnopharmacological relevance
The leaves of Calpurnia aurea (Ait.) Benth are used for wound healing activities through topical application in different communities in Ethiopia. The leaves had many confirmed in vitro activities that can promote wound healing effects. In spite of many claims and in vitro studies with supportive results in wound healing, no scientific study has been conducted on the wound healing and anti-inflammatory activity of leaves of Calpurnia aurea (Ait.) Benth on animals.
Aim of the study
was to evaluate the wound healing and anti-inflammatory activity of leaves of Calpurnia aurea (Ait.) Benth scientifically in mice.
Methods and materials
Air dried leaves of Calpurnia aurea (Ait.) Benth were grounded and macerated three times successively by 80% methanol. Then part of the dried extract was fractionated with chloroform, ethyl acetate and aqueous. Phytochemical screening tests were performed according standard test procedures. The crude extract and solvent fractions were formulated to ointments. The acute dermal toxicity of the crude extract was determined in rats. Wound healing activity of the crude extract was evaluated using excision and incision wound models and the wound healing activities of solvent fractions were evaluated by using excision wound model. The anti-inflammatory activity of the 80% methanol extract of leaves of Calpurnia aurea (Ait.) Benth was evaluated using carrageenan induced hind paw edema model in mice.
Results
The 2000 mg/kg test dose of the 10% w/w crude extract ointment was safe in rats. The 5% w/w and 10% w/w crude extract ointments revealed significant (p < 0.001) wound contraction from day 4-day 8 post wounding. The 10% w/w crude extract ointment revealed a significant (p < 0.01) shortening of epithelialization period in excision wound model. Both 5% w/w and 10% w/w crude extract ointments showed significant (p < 0.001) increment of tensile strength compared to the negative control. The 10% w/w aqueous fraction ointment revealed high (p < 0.001) percentage of wound contraction and reduced (p < 0.001) period of epithelialization. The 100 mg/kg, 200 mg/kg and 400 mg/kg oral administration of the crude extract had significant inhibition of the paw edema in mice of carrageenan induced inflammation.
Conclusion
The results of this study demonstrated that 80% methanol extract of the leaves of Calpurnia aurea (Ait.) Benth exhibited wound healing and anti-inflammatory effects. The aqueous and ethyl acetate fractions possessed wound healing activities.
{"title":"Evaluation of wound healing and anti-inflammatory activity of the leaves of Calpurnia aurea (Ait.) Benth (fabaceae) in mice","authors":"Getachew Ayal , Assefa Belay , Wubayehu Kahaliw","doi":"10.1016/j.wndm.2019.100151","DOIUrl":"10.1016/j.wndm.2019.100151","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><p>The leaves of <em>Calpurnia aurea</em> (Ait.) Benth are used for wound healing activities through topical application in different communities in Ethiopia. The leaves had many confirmed in vitro activities that can promote wound healing effects. In spite of many claims and in vitro studies with supportive results in wound healing, no scientific study has been conducted on the wound healing and anti-inflammatory activity of leaves of <em>Calpurnia aurea</em> (Ait.) Benth on animals.</p></div><div><h3>Aim of the study</h3><p>was to evaluate the wound healing and anti-inflammatory activity of leaves of <em>Calpurnia aurea</em> (Ait.) Benth scientifically in mice.</p></div><div><h3>Methods and materials</h3><p>Air dried leaves of <em>Calpurnia aurea</em> (Ait.) Benth were grounded and macerated three times successively by 80% methanol. Then part of the dried extract was fractionated with chloroform, ethyl acetate and aqueous. Phytochemical screening tests were performed according standard test procedures. The crude extract and solvent fractions were formulated to ointments. The acute dermal toxicity of the crude extract was determined in rats. Wound healing activity of the crude extract was evaluated using excision and incision wound models and the wound healing activities of solvent fractions were evaluated by using excision wound model. The anti-inflammatory activity of the 80% methanol extract of leaves of <em>Calpurnia aurea</em> (Ait.) Benth was evaluated using carrageenan induced hind paw edema model in mice.</p></div><div><h3>Results</h3><p>The 2000 mg/kg test dose of the 10% w/w crude extract ointment was safe in rats. The 5% w/w and 10% w/w crude extract ointments revealed significant (p < 0.001) wound contraction from day 4-day 8 post wounding. The 10% w/w crude extract ointment revealed a significant (p < 0.01) shortening of epithelialization period in excision wound model. Both 5% w/w and 10% w/w crude extract ointments showed significant (p < 0.001) increment of tensile strength compared to the negative control. The 10% w/w aqueous fraction ointment revealed high (p < 0.001) percentage of wound contraction and reduced (p < 0.001) period of epithelialization. The 100 mg/kg, 200 mg/kg and 400 mg/kg oral administration of the crude extract had significant inhibition of the paw edema in mice of carrageenan induced inflammation.</p></div><div><h3>Conclusion</h3><p>The results of this study demonstrated that 80% methanol extract of the leaves of <em>Calpurnia aurea</em> (Ait.) Benth exhibited wound healing and anti-inflammatory effects. The aqueous and ethyl acetate fractions possessed wound healing activities.</p></div>","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"25 1","pages":"Article 100151"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42740897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.1016/j.wndm.2019.100155
Saeid Vakilian , Fatemeh Jamshidi-adegani , Sulaiman Al-Shidhani , Muhammad U. Anwar , Rashid Al-Harrasi , Nasar Al-Wahaibi , Asim Qureshi , Sausan Alyaqoobi , Issa Al-Amri , Ahmed Al-Harrasi , Sulaiman Al-Hashmi
Keratin-based biomaterials can be considered as the beneficial platform for designing suitable wound dressers. One of the most common natural materials that are composed of keratin and lipid is snakes shed skin. In this new study, shed skin of two different Omani snakes; Bitis arietans snake (Puff adder, “P”) and Telescopus dhara snake (Arabian Cat snake, “C”); were examined as wound dresser. The presence of lipid and keratin were assessed using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR). 3- Layered filamentous morphology of the P shed skin and the 3-layered compact structure of C shed skin were analyzed by Scanning Electron Microscopy (SEM). X-Ray powder Diffraction (XRD) analysis proved two main peaks corresponding to the α-helix and β-sheet of the protein. Crystallinity index (CI) of P and C shed skins were obtained from XRD peaks 42.85 and 28.57%. The in vivo and histopathological results indicated that skin reconstruction was effectively improved under P shed skin treatment as compared with negative and positive control (PC) and C groups. Superior histopathological scores were the beneficial properties of P group over the positive control. The synergistic effect of natural extracellular matrix-mimicking structure and keratin beneficial proteins for wound healing could develop a natural substrate for wound healing in the clinical setting.
{"title":"A Keratin-based biomaterial as a promising dresser for skin wound healing","authors":"Saeid Vakilian , Fatemeh Jamshidi-adegani , Sulaiman Al-Shidhani , Muhammad U. Anwar , Rashid Al-Harrasi , Nasar Al-Wahaibi , Asim Qureshi , Sausan Alyaqoobi , Issa Al-Amri , Ahmed Al-Harrasi , Sulaiman Al-Hashmi","doi":"10.1016/j.wndm.2019.100155","DOIUrl":"10.1016/j.wndm.2019.100155","url":null,"abstract":"<div><p>Keratin-based biomaterials can be considered as the beneficial platform for designing suitable wound dressers. One of the most common natural materials that are composed of keratin and lipid is snakes shed skin. In this new study, shed skin of two different Omani snakes; Bitis arietans snake (Puff adder, “P”) and Telescopus dhara snake (Arabian Cat snake, “C”); were examined as wound dresser. The presence of lipid and keratin were assessed using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR). 3- Layered filamentous morphology of the P shed skin and the 3-layered compact structure of C shed skin were analyzed by Scanning Electron Microscopy (SEM). X-Ray powder Diffraction (XRD) analysis proved two main peaks corresponding to the α-helix and β-sheet of the protein. Crystallinity index (CI) of P and C shed skins were obtained from XRD peaks 42.85 and 28.57%. The in vivo and histopathological results indicated that skin reconstruction was effectively improved under P shed skin treatment as compared with negative and positive control (PC) and C groups. Superior histopathological scores were the beneficial properties of P group over the positive control. The synergistic effect of natural extracellular matrix-mimicking structure and keratin beneficial proteins for wound healing could develop a natural substrate for wound healing in the clinical setting.</p></div>","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"25 1","pages":"Article 100155"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41847632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Several plant preparations and extracts are used in folk medicine for treatment of topical wounds. Yet the validity of their use as well as the mechanism has not been ascertained in well-designed experimental settings. The wound healing activity of a topical hexane extract of Alkanna strigosa roots (HEASR) in excision and incision wound models in rats was evaluated. The pro-healing activity was assessed by the rate of wound contraction & wet granulation tissue weight and tensile strength in the excision and incision models, respectively.
The active components of HEASR were isolated. Finally, the antimicrobial activity against five microorganisms; namely Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Candida albicans were assessed for both crude extract and pure compounds.
The HEASR increased the weight of wet granulation tissue per wound area and tensile strength of wounds, and showed antimicrobial activity against Gram-positive bacteria and C. albicans. The pure compounds were alkanin & shikonin and showed remarkable activity against S. aureus, E. coli and B. subtilis. The results document the beneficial effect of A. strigosa extract and its pure compounds for treating the supurative excision as well as the incision wounds.
{"title":"In vivo wound healing and antimicrobial activity of Alkanna strigose","authors":"Talal Aburjai , Reem Al-Janabi , Farah Al-Mamoori , Hanan Azzam","doi":"10.1016/j.wndm.2019.100152","DOIUrl":"10.1016/j.wndm.2019.100152","url":null,"abstract":"<div><p><span>Several plant preparations<span><span> and extracts are used in folk medicine for treatment of topical wounds. Yet the validity of their use as well as the mechanism has not been ascertained in well-designed experimental settings. The wound healing activity of a topical </span>hexane extract of </span></span><em>Alkanna strigosa</em><span><span> roots (HEASR) in excision and incision wound models in rats was evaluated. The pro-healing activity was assessed by the rate of </span>wound contraction & wet granulation tissue weight and tensile strength in the excision and incision models, respectively.</span></p><p><span>The active components of HEASR were isolated. Finally, the antimicrobial activity against five microorganisms; namely </span><span><em>Staphylococcus aureus</em></span>, <span><em>Pseudomonas aeruginosa</em></span>, <em>Escherichia coli</em>, <span><em>Bacillus subtilis</em></span> and <em>Candida albicans</em> were assessed for both crude extract and pure compounds.</p><p>The HEASR increased the weight of wet granulation tissue per wound area and tensile strength of wounds, and showed antimicrobial activity against Gram-positive bacteria and <em>C. albicans</em><span>. The pure compounds were alkanin & shikonin and showed remarkable activity against </span><em>S. aureus</em>, <em>E. coli</em> and <em>B. subtilis</em>. The results document the beneficial effect of <em>A. strigosa</em> extract and its pure compounds for treating the supurative excision as well as the incision wounds.</p></div>","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"25 1","pages":"Article 100152"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.100152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44719162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-01DOI: 10.1016/j.wndm.2019.02.004
Adam Węgrzynowski, Mikołaj Kamiński, Piotr Liszkowski, Jacek Soska, Aleksandra Araszkiewicz, Dorota Zozulińska-Ziółkiewicz
Background
The University of Texas Wound Classification is a tool assesing risk of amputation of patients with Diabetic Foot Ulceration (DFU). Negative Pressure Wound Therapy (NPWT) is a nonivasive system which was shown to be effective in healing chronic wounds. The aim of the study was to assess utility of long-term NPWT in healing diabetic foot wounds among Polish patients with diabetes.
Material & Methods
In the study, we retrospectively analysed data of patients of DFU ambulatory using NPWT. Collected data included sex, age, type of DFU, duration of NPWT, history of previous minor amputation and characteristic of the healed wound in The University of Texas Wound Classification before and after NPWT.
Results
Total number of participants counted 21 (male = 16, 76%). The wound significantly improved in 17 (81%) patients. Group with successful treatment reached median 92% decrease of risk of amputation (-8.5 to -92%; p < 0.001). Group with successful treatment differed from group with unsuccessful treatment in count of angiopathic DFU (Chi2, 4[24%] vs. 3[75%]; p = 0.049). No significant differences in age, sex, history of previous minor amputation, presence of infection, depth of the ulcer were found. No NPWT adverse effect were reported. Logistic regression model revealed significant relationship between unsuccessful outcome of NPWT and presence of ischemic ulcer adjusted to presence of infection, depth of a wound and sex (OR = 27.5; CI: 1.1–716.7; p = 0.046).
Conclusions
NPWT significantly decraeses risk of amputation in Texas Score. NPWT may not help in healing wounds simultaneously infected and ischeamic. Presence of ischemic wound decreases chance for successful outcome.
{"title":"Long-term negative pressure wound therapy decreases a risk of diabetic foot amputation assessed in the university of Texas wound classification","authors":"Adam Węgrzynowski, Mikołaj Kamiński, Piotr Liszkowski, Jacek Soska, Aleksandra Araszkiewicz, Dorota Zozulińska-Ziółkiewicz","doi":"10.1016/j.wndm.2019.02.004","DOIUrl":"10.1016/j.wndm.2019.02.004","url":null,"abstract":"<div><h3>Background</h3><p>The University of Texas Wound Classification is a tool assesing risk of amputation of patients with Diabetic Foot Ulceration (DFU). Negative Pressure Wound Therapy (NPWT) is a nonivasive system which was shown to be effective in healing chronic wounds. The aim of the study was to assess utility of long-term NPWT in healing diabetic foot wounds among Polish patients with diabetes.</p></div><div><h3>Material & Methods</h3><p>In the study, we retrospectively analysed data of patients of DFU ambulatory using NPWT. Collected data included sex, age, type of DFU, duration of NPWT, history of previous minor amputation and characteristic of the healed wound in The University of Texas Wound Classification before and after NPWT.</p></div><div><h3>Results</h3><p>Total number of participants counted 21 (male = 16, 76%). The wound significantly improved in 17 (81%) patients. Group with successful treatment reached median 92% decrease of risk of amputation (-8.5 to -92%; p < 0.001). Group with successful treatment differed from group with unsuccessful treatment in count of angiopathic DFU (Chi2, 4[24%] vs. 3[75%]; p = 0.049). No significant differences in age, sex, history of previous minor amputation, presence of infection, depth of the ulcer were found. No NPWT adverse effect were reported. Logistic regression model revealed significant relationship between unsuccessful outcome of NPWT and presence of ischemic ulcer adjusted to presence of infection, depth of a wound and sex (OR = 27.5; CI: 1.1–716.7; p = 0.046).</p></div><div><h3>Conclusions</h3><p>NPWT significantly decraeses risk of amputation in Texas Score. NPWT may not help in healing wounds simultaneously infected and ischeamic. Presence of ischemic wound decreases chance for successful outcome.</p></div>","PeriodicalId":38278,"journal":{"name":"Wound Medicine","volume":"24 1","pages":"Pages 33-35"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.wndm.2019.02.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44788657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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