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DISTRIBUTION AND SEVERITY OF APICAL ROOT RESORPTION IN INFLAMMATORY PERIAPICAL PATHOLOGIES AMONGST DIABETIC AND NON DIABETIC PATIENTS – A PILOT STUDY 糖尿病和非糖尿病患者根尖周围炎症病理中根尖吸收的分布和严重程度-一项初步研究
Q4 Dentistry Pub Date : 2020-04-10 DOI: 10.46791/ujmds.2020.080102
B. Durgadevi, Jimsha Vk, Srinivasan Sv, M JonathanDaniel
Distribution and severity of apical root resorption in inflammatory periapical pathologies amongst diabetic and non diabetic
糖尿病和非糖尿病患者根尖周围炎症病理中根尖吸收的分布和严重程度
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引用次数: 0
IRRITABLE BOWEL SYNDROME (IBS) - A LITERARY REVIEW 肠易激综合征&文献综述
Q4 Dentistry Pub Date : 2020-04-10 DOI: 10.46791/ujmds.2020.080103
Gaikwad Sanjay R, Kakde Vishwanath B
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引用次数: 0
BUTTON POUCH TECHNIQUE WITH LASER TREATED PRF FOR TREATMENT OF MULTIPLE GINGIVAL RECESSIONS - A CASE REPORT 钮扣袋技术结合激光治疗PRF治疗多发性牙龈萎缩1例
Q4 Dentistry Pub Date : 2020-04-10 DOI: 10.46791/ujmds.2020.080101
Keerthi Kl, P AravindKumar, M. NarendraBabu, Kalapala Raviraj
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引用次数: 0
MANAGEMENT OF ELLIS CLASS VIII FRACTURE - A SURGICAL APPROACH Ellis viii级骨折的手术治疗
Q4 Dentistry Pub Date : 2020-04-10 DOI: 10.46791/ujmds.2020.080105
M. K. Sagar, T. Priyanka, M. Satish, Shinde Christopher Vinay, Dubey Ashutosh, N. Babita
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引用次数: 0
Optimal Initial Cell Density that Yields the Highest Number of Primary Synovial Mesenchymal Stem Cells in a Clinical Setting 在临床环境中产生最多数量原代滑膜间充质干细胞的最佳初始细胞密度
Q4 Dentistry Pub Date : 2020-04-09 DOI: 10.21203/rs.3.rs-21533/v1
Kiyotaka Horiuchi, M. Mizuno, H. Katano, Kentaro Endo, Nobutale Ozeki, K. Tsuji, H. Koga, I. Sekiya
Background Synovial mesenchymal stem cell (MSC) transplantation improved clinical outcomes in knees with cartilage defects and after repairs of degenerative torn menisci. For these, nucleated cells were cultured with 10% autologous serum for 14 days. Importantly, nucleated cell number and serum volume practically varied by patient, and initial cell density and dish number affected yield/donor. Our purpose was to examine the effect of plating density within range in clinical studies on fold increase and to estimate the MSC yield/donor, which are affected by the nucleated cell number and serum volume.Methods We obtained the data of the autologous serum volume, nucleated cell number, and initial plating cell density from 10 patients who participated in our previous clinical studies. Experimentally, synovial nucleated cells harvested from three patients with osteoarthritis during total knee arthroplasty were cultured with 10% mixed allogenic human serum for 14 days to examine the effect of initial cell density on fold increase. We used both our previous clinical data and our current experimental data to predict the yields of synovial MSCs in nine cases within the range of clinical studies.Results In the clinical studies, collected serum ranged between 87 and 186 ml, harvested nucleated cells ranged between 6.2 and 23 million, and the top five total MSC yields were obtained from donors when the initial plating cell density ranged from 919 to 1905 cells/cm2. Experimentally, fold increase significantly decreased between 700 and 1,900 cells/cm2. In cases where the cell number was low and the serum volume was high, the estimated yield/donor decreased along with the initial density, while in cases where the cell number was high and the serum volume was low, the estimated yield/donor increased along with the initial density. In cases both where some nucleated cells were discarded at low density and no cells were discarded at high density, the maximum yield/donor were obtained when the initial density was the lowest density that does not discard cells.Conclusions There was an optimal initial density that yields the highest number of primary MSCs in a clinical setting when cell numbers and human serum volumes are limited.
背景滑膜间充质干细胞(MSC)移植改善了膝关节软骨缺损和退行性半月板撕裂修复后的临床疗效。对于这些,将有核细胞与10%自体血清培养14天。重要的是,有核细胞数量和血清体积实际上因患者而异,初始细胞密度和培养皿数量影响产量/供体。我们的目的是检查临床研究中范围内的电镀密度对倍数增加的影响,并估计MSC产量/供体,这受到有核细胞数量和血清体积的影响。方法从10例参与临床研究的患者中获得自体血清体积、有核细胞数和初始细胞密度的数据。实验上,在全膝关节置换术期间从三名骨关节炎患者身上采集的滑膜有核细胞与10%混合同种异体人血清培养14天,以检查初始细胞密度对倍数增加的影响。我们使用我们以前的临床数据和目前的实验数据来预测临床研究范围内9例滑膜间充质干细胞的产量。结果在临床研究中,收集的血清范围在87至186毫升之间,收获的有核细胞范围在620至2300万之间,当初始培养细胞密度在919至1905个细胞/cm2之间时,从供体获得了前五名的MSC总产量。在实验中,在700和1900个细胞/cm2之间,倍数增加显著降低。在细胞数量低且血清体积高的情况下,估计产量/供体随着初始密度而降低,而在细胞数量高且血清体积低的情况下估计产量/受体随着初始密度增加。在一些有核细胞以低密度被丢弃和没有细胞以高密度被丢弃的情况下,当初始密度是不丢弃细胞的最低密度时,获得最大产量/供体。结论当细胞数量和人血清体积有限时,在临床环境中存在产生最高数量原代间充质干细胞的最佳初始密度。
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引用次数: 2
Solute carrier transporters, reduced folate carrier 1 and equilibrative nucleoside transporter 1, as immunohistochemical markers for high-grade malignancy in bladder cancer 可溶性载体转运蛋白、还原叶酸载体1和平衡核苷转运蛋白1作为癌症高级别恶性肿瘤的免疫组织化学标志物
Q4 Dentistry Pub Date : 2020-02-01 DOI: 10.11480/JMDS.670002
Denda Tamami, Kamoshida Shingo, Kuwao Sadahito, Kawamura Jumpei, Ota Yasunori, Soejima Yurie, Akiyama Masako, Sawabe Motoji
Clinicopathological parameters derived from initial transurethral resection of bladder tumor (TUR-Bt) have limitations in predicting tumor progression in bladder cancer. Reduced folate carrier 1 (RFC1) and equilibrative nucleoside transporter 1 (ENT1) are solute carrier (SLC) transporters supporting cellular uptake of endogenous bioactive substances and anti-cancer drugs. The aim of this study was to elucidate the role of SLC transporters in bladder cancer and investigate the potential of RFC1 and ENT1 expression as immunohistochemical markers for high-grade malignancy. We compared T-stage with the immunohistochemical expression of RFC1 and ENT1 and other clinicopathological parameters; moreover, we also used multiple logistic regression model to assess relative contributions for T-stage in bladder cancer (n=130). Concurrently, 57 TUR-Bt-derived imprint cytological samples were stained to evaluate the implication of cytological analysis. Elevated expression levels of RFC1 and ENT1 were significantly correlated with higher T-stage (p < .0001) and efficiently predicted tumor progres sion, compared with other clinicopathological parameters (RFC1, p = .0325; ENT1, p = .0171). Indepen dent variables of optimal model for predicting T-stage were gender, age, histological grade, expression levels of RFC1 and ENT1. Cytological analysis was consistent with immunostained-tissue data. We reveal RFC1 and ENT1 as potential immunohistocytochemical markers for high-grade malignancy in bladder cancer.
来自初次经尿道膀胱肿瘤切除术(TUR-Bt)的临床病理参数在预测癌症的肿瘤进展方面具有局限性。还原叶酸载体1(RFC1)和平衡核苷转运蛋白1(ENT1)是支持细胞摄入内源性生物活性物质和抗癌药物的溶质载体(SLC)转运蛋白。本研究的目的是阐明SLC转运蛋白在膀胱癌症中的作用,并研究RFC1和ENT1表达作为高级别恶性肿瘤免疫组织化学标志物的潜力。我们将T分期与RFC1和ENT1的免疫组织化学表达以及其他临床病理参数进行了比较;此外,我们还使用多元逻辑回归模型来评估癌症T分期的相对贡献(n=130)。同时,对57份TUR-Bt来源的印迹细胞学样本进行染色,以评估细胞学分析的意义。与其他临床病理参数(RFC1,p=.0325;ENT1,p=.0171)相比,RFC1和ENT1的表达水平升高与较高的T分期显著相关(p<.0001),并有效预测肿瘤进展。预测T分期的最佳模型的独立变量为性别、年龄、组织学分级、RFC1和ENT1的表达量。细胞学分析与免疫染色的组织数据一致。我们发现RFC1和ENT1是癌症高级别恶性肿瘤的潜在免疫组织细胞化学标志物。
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引用次数: 0
Application of cell block preparation in effusion cytology: Analysis of mismatched diagnosis and utility of immunostaining 细胞块制备在积液细胞学中的应用:误诊分析及免疫染色的应用
Q4 Dentistry Pub Date : 2020-02-01 DOI: 10.11480/JMDS.670003
A. Horiguchi, Takashi Umezawa, Miyaka Umemori, S. Ito, Sachiko Tsuchiya, S. Hirooka, T. Kiyokawa, M. Ikegami, Hiroyuki Takahashi, Yurie Soejima, M. Sawabe
Objective: To analyze the mismatched cases between liquid-based cytology (LBC) and cell block in effusion cytology and to confirm the utility of cell block. Methods: One hundred eighty-two samples of effusions were examined. Cell blocks were prepared from residual samples after LBC preparation, and the details about the diagnostic concordance and difference in cytological characteristics were investigated. Cell block immunostaining was performed to predict the histological type and the primary site of the carcinoma in 32 cases. ALK rearrangement and EGFR mutation were also analyzed using the cell block. Results: The diagnostic concordance rate between LBC and cell block was 97.3%. Diagnoses using LBC and cell block were mismatched in five cases. By immunostaining, the histological type was determined in 91.0% of carcinomas, and primary sites were identified in 76.5% of adenocarcinomas. ALK rearrangement was examined in two cases of lung carcinoma and EGFR mutation was examined in four cases of lung carcinoma. Conclusion: A high concordance rate between LBC and cell block in effusion cytology was found. The main cause of the mismatched diagnosis was the small amount of atypical cells in LBC or cell block. We also showed the utility of cell block in immunostaining and DNA analysis. Key word: cell block, liquid-based cytology, pleural effusion, peritoneal effusion, immunohistochemistry.
目的:分析积液细胞学中液基细胞学(LBC)与细胞阻滞不匹配的病例,并证实细胞阻滞的实用性。方法:对182例渗出液标本进行检查。从LBC制备后的残余样本中制备细胞块,并研究细胞学特征的诊断一致性和差异性的细节。用细胞块免疫染色法预测32例肿瘤的组织学类型和原发部位。ALK重排和EGFR突变也使用细胞块进行分析。结果:LBC和细胞阻滞的诊断符合率为97.3%,其中5例LBC与细胞阻滞诊断不匹配。通过免疫染色,91.0%的癌具有组织学类型,76.5%的腺癌具有原发灶。在2例肺癌中检测了ALK重排,在4例肺癌中检查了EGFR突变。结论:积液细胞学检查发现LBC与细胞阻滞的符合率较高。诊断不匹配的主要原因是LBC或细胞阻滞中有少量非典型细胞。我们还展示了细胞块在免疫染色和DNA分析中的作用。关键词:细胞块,液基细胞学,胸腔积液,腹膜积液,免疫组织化学。
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引用次数: 0
Effectiveness of health education programme of primary school-aged children in the urban area of China 中国城市地区小学学龄儿童健康教育方案的效果
Q4 Dentistry Pub Date : 2020-01-01 DOI: 10.11480/JMDS.680004
Xiaowei Lyu, Kumiko Morita, Hitomi Nagamine, Kay Maruyama
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引用次数: 0
Association between combinations of pathological conditions causing cerebral palsy among mothers and infants and associated characteristics including umbilical arterial pH and parity 导致母婴脑瘫的病理条件组合与相关特征(包括脐带动脉pH值和胎次)之间的关联
Q4 Dentistry Pub Date : 2020-01-01 DOI: 10.11480/JMDS.680001
M. Murata, M. Sugawa, K. Kawahara
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引用次数: 0
Cluster analysis of patients with cough, mainly cough variant asthma, showing a good response to asthma therapy 对咳嗽患者进行聚类分析,以咳嗽变异性哮喘为主,对哮喘治疗反应良好
Q4 Dentistry Pub Date : 2020-01-01 DOI: 10.11480/JMDS.670001
Saki Oyama, Y. Otani, Wakaba Furuie, Fumika Koike, Takao Miyoshi, M. Akaza, Y. Sumi
Introduction: Cough variant asthma (CVA) and asthma account for a large proportion of patients with chronic cough. We used hierarchical cluster analysis (HCA) to characterize the phenotypes of patients with cough, mainly those with CVA, who showed a rapid response to asthma therapy, and sought to identify simple diagnostic markers. Methods: Patients who initially presented with cough as the main symptom were retrospectively enrolled. Those who were clinically diagnosed with asthma/CVA were selected and analyzed. HCA was performed and the patients were classified by phenotype. Results: HCA identified the five following phenotypes among the 221 participants: highly eosinophilic bronchitis [high fractional exhaled nitric oxide (FeNO) , n = 28]; atopic [high total serum IgE , n = 23]; non-atopic and non-eosinophilic [n = 104]; distal airway narrowing [low FEF75 (V ・ 25), n = 21] and severely atopic [extremely high total serum IgE and/or extreme high FeNO, n = 45]. No significant differences in long-term prognosis were found among the phenotypes. Discussion: Five phenotypes were identified among patients with cough who showed a rapid response to asthma treatment. Wheezing was observed in about 24% of patients, and abnormal FeNO or V ・ 25 levels in about 30%.
简介:咳嗽变异性哮喘(Cough variant asthma, CVA)和哮喘在慢性咳嗽患者中占很大比例。我们使用层次聚类分析(HCA)来表征咳嗽患者的表型,主要是CVA患者,他们对哮喘治疗表现出快速反应,并试图确定简单的诊断标记。方法:对以咳嗽为主要症状的患者进行回顾性分析。选择临床诊断为哮喘/CVA的患者进行分析。采用HCA法对患者进行表型分类。结果:HCA在221名参与者中确定了以下五种表型:高度嗜酸性支气管炎[高分数呼出一氧化氮(FeNO), n = 28];特应性[血清总IgE高,n = 23];非特应性和非嗜酸性粒细胞[n = 104];远端气道狭窄[低FEF75 (V·25),n = 21]和严重特应性[极高血清总IgE和/或极高FeNO, n = 45]。各表型间长期预后无显著差异。讨论:在对哮喘治疗有快速反应的咳嗽患者中发现了五种表型。约24%的患者出现喘息,约30%的患者出现FeNO或V·25水平异常。
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引用次数: 0
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Journal of Medical and Dental Sciences
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