首页 > 最新文献

Annales Bogorienses-Journal of Tropical General Botany最新文献

英文 中文
Partial Purification, Characterization, and Application of Extracellular Aspartic Protease from Lactobacillus casei WSP in Producing the Bioactive Peptides with Antibacterial and Antioxidant Activity 干酪乳杆菌胞外天冬氨酸蛋白酶的部分纯化、鉴定及在生产具有抗菌和抗氧化活性肽中的应用
Pub Date : 2018-12-31 DOI: 10.14203/ANN.BOGOR.2018.V22.N2.47-56
A. Solikhin, A. Z. Mustopa, S. Suharsono, W. Putranto
   Lactobacillus casei WSP-derived an aspartic protease was sequentially purified by using chromatography gel filtration sephadex G-50. It resulted in a 22.81-fold increase of specific activity (51.5 U/mg) with a final yield of 1.9%. The estimated molecular weight of the purified enzyme was 37 kDa and showed gelatinolytic activity in zymogram assay. The enzyme exhibited optimum activity at 40ºC and pH 6 with casein as the substrate. Enzyme activity was significantly inhibited by pepstatin A (0.5 mM and 1 mM), confirming that this enzyme is a group of aspartic proteases, while other inhibitors such as EDTA, PMSF and iodoacetic acid showed no inhibition effect on the activity of enzyme. The addition of metal ion to the enzyme decreased enzyme activity, indicating the proteolytic enzyme was metal ion- dependent. Denaturant such as DDT tended to increase caseinolytic activity. Furthermore, this enzyme was capable of generating the new peptides from skimmed milk with the size 8 kDa, 10 kDa and 15 kDa. These peptides have potential as antibacterial and antioxidant agents.
采用sephadex G-50层析凝胶过滤法对干酪乳杆菌wsp衍生的天冬氨酸蛋白酶进行了纯化。比活性提高22.81倍(51.5 U/mg),最终产率为1.9%。酶谱分析表明,纯化酶的分子量为37 kDa,具有溶胶活性。以酪蛋白为底物,在40℃和pH 6条件下酶活性最佳。酶活性被pepstatin A (0.5 mM和1 mM)显著抑制,证实该酶是一组天冬氨酸蛋白酶,而其他抑制剂如EDTA、PMSF和碘乙酸对酶活性无抑制作用。金属离子的加入降低了酶的活性,表明该蛋白水解酶是金属离子依赖性的。变性剂如滴滴涕倾向于增加溶酪蛋白活性。此外,该酶还能从脱脂牛奶中生成8kda、10kda和15kda的新肽。这些肽具有抗菌和抗氧化剂的潜力。
{"title":"Partial Purification, Characterization, and Application of Extracellular Aspartic Protease from Lactobacillus casei WSP in Producing the Bioactive Peptides with Antibacterial and Antioxidant Activity","authors":"A. Solikhin, A. Z. Mustopa, S. Suharsono, W. Putranto","doi":"10.14203/ANN.BOGOR.2018.V22.N2.47-56","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2018.V22.N2.47-56","url":null,"abstract":"   Lactobacillus casei WSP-derived an aspartic protease was sequentially purified by using chromatography gel filtration sephadex G-50. It resulted in a 22.81-fold increase of specific activity (51.5 U/mg) with a final yield of 1.9%. The estimated molecular weight of the purified enzyme was 37 kDa and showed gelatinolytic activity in zymogram assay. The enzyme exhibited optimum activity at 40ºC and pH 6 with casein as the substrate. Enzyme activity was significantly inhibited by pepstatin A (0.5 mM and 1 mM), confirming that this enzyme is a group of aspartic proteases, while other inhibitors such as EDTA, PMSF and iodoacetic acid showed no inhibition effect on the activity of enzyme. The addition of metal ion to the enzyme decreased enzyme activity, indicating the proteolytic enzyme was metal ion- dependent. Denaturant such as DDT tended to increase caseinolytic activity. Furthermore, this enzyme was capable of generating the new peptides from skimmed milk with the size 8 kDa, 10 kDa and 15 kDa. These peptides have potential as antibacterial and antioxidant agents.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"14 10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82570607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
DNA Condensation Study of Fully Synthesized Lipopeptide-Based Transfection Agent for Gene Delivery Vehicle 全合成脂肽基因载体转染剂的DNA缩合研究
Pub Date : 2018-12-31 DOI: 10.14203/ANN.BOGOR.2018.V22.N2.65-74
Tarwadi Tarwadi, H. Rachmawati, R. E. Kartasasmita, S. Pambudi, A. D. Arbianto, D. Restiani, Sukmadjaja Asyarie
   The main requirement of transfection agent has to condense DNA in nanoparticle size, protect the DNA from nucleases and other degrading enzymes during its transport in cell cytoplasm and nucleus and should not toxic to target cells. In this research, lipopeptide composed of palmitoyl (C-16) and short peptide sequence have been designed fully synthesized and tested to DNA condensation capability and toxicity. The DNA condensation study was performed using EtBr exclusion assay and cytotoxicity determination was carried out by colorimetric MTT assay. It was revealed that lipopeptide-based transfection agent of Pal-CKKHH and Pal-CKKHH-YGRKKRRQRRR-PKKKRKV condensed DNA molecules efficiently. The lipopeptide was less toxic compared to Lipofectamine and Poly-L-Lysine, that shown by 90% of CHO-K1 cells remained viable when they were treated with 4.36 µM Pal-CKKHHYGRKKRRQRRR-PKKKRKV. Meanwhile, there were only ~75% and 80% of CHO-K1 viable cells when it was treated with PLL and Lipofectamine®2000, respectively. Moreover, cell viability of HepG2 was ~ 75% after treated with 2.18 µM of Pal-CKKHH-YGRKKRRQRRR-PKKKRKV and decreased to ~65% when the lipopeptide concentration increased to 8.72 M. In summary, the synthesized lipopeptide condenses DNA molecules efficiently, less toxic than Lipofectamine®2000 and PLL and has possibility to be explored as a non-viral gene delivery vehicle.
转染剂的主要要求是将DNA浓缩到纳米颗粒大小,保护DNA在细胞质和细胞核中运输过程中不受核酸酶和其他降解酶的影响,并且对靶细胞无毒。本研究设计合成了棕榈酰(C-16)和短肽序列组成的脂肽,并对其DNA缩聚能力和毒性进行了测试。DNA缩聚研究采用EtBr排除法,细胞毒性测定采用比色MTT法。结果表明,基于脂肽的Pal-CKKHH和Pal-CKKHH- ygrkkrrqrrr - pkkkrkv转染剂能有效地凝聚DNA分子。与脂质胺和聚l-赖氨酸相比,脂肽的毒性更小,这表明当4.36µM Pal-CKKHHYGRKKRRQRRR-PKKKRKV处理CHO-K1细胞时,90%的CHO-K1细胞仍然存活。同时,用PLL和Lipofectamine®2000处理CHO-K1时,分别只有~75%和80%的活细胞。此外,2.18µM Pal-CKKHH-YGRKKRRQRRR-PKKKRKV处理后,HepG2细胞存活率为~ 75%,而当脂肽浓度增加到8.72 M时,HepG2细胞存活率下降至~65%。总之,合成的脂肽能有效凝聚DNA分子,毒性低于Lipofectamine®2000和PLL,有可能作为非病毒基因传递载体进行探索。
{"title":"DNA Condensation Study of Fully Synthesized Lipopeptide-Based Transfection Agent for Gene Delivery Vehicle","authors":"Tarwadi Tarwadi, H. Rachmawati, R. E. Kartasasmita, S. Pambudi, A. D. Arbianto, D. Restiani, Sukmadjaja Asyarie","doi":"10.14203/ANN.BOGOR.2018.V22.N2.65-74","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2018.V22.N2.65-74","url":null,"abstract":"   The main requirement of transfection agent has to condense DNA in nanoparticle size, protect the DNA from nucleases and other degrading enzymes during its transport in cell cytoplasm and nucleus and should not toxic to target cells. In this research, lipopeptide composed of palmitoyl (C-16) and short peptide sequence have been designed fully synthesized and tested to DNA condensation capability and toxicity. The DNA condensation study was performed using EtBr exclusion assay and cytotoxicity determination was carried out by colorimetric MTT assay. It was revealed that lipopeptide-based transfection agent of Pal-CKKHH and Pal-CKKHH-YGRKKRRQRRR-PKKKRKV condensed DNA molecules efficiently. The lipopeptide was less toxic compared to Lipofectamine and Poly-L-Lysine, that shown by 90% of CHO-K1 cells remained viable when they were treated with 4.36 µM Pal-CKKHHYGRKKRRQRRR-PKKKRKV. Meanwhile, there were only ~75% and 80% of CHO-K1 viable cells when it was treated with PLL and Lipofectamine®2000, respectively. Moreover, cell viability of HepG2 was ~ 75% after treated with 2.18 µM of Pal-CKKHH-YGRKKRRQRRR-PKKKRKV and decreased to ~65% when the lipopeptide concentration increased to 8.72 M. In summary, the synthesized lipopeptide condenses DNA molecules efficiently, less toxic than Lipofectamine®2000 and PLL and has possibility to be explored as a non-viral gene delivery vehicle.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82504584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular Evaluation for Drought Tolerant Using Marker Assisted Breeding Method 利用标记辅助育种方法进行耐旱性分子评价
Pub Date : 2018-12-31 DOI: 10.14203/ann.bogor.2018.v22.n2.94-100
F. Fatimah, J. Prasetiyono, K. Trijatmiko, Sustiprijatno Sustiprijatno
   The sustainability and increasing the national rice production require the readiness of food and agriculture sector cope with the impacts of climate change, land degradation, drought area, sloping production and the raising of population growth. Adaptation plays an important role in ensuring the sustainability of food security. This research aimed to develop drought-tolerant variety of Inpari 30 (submergence tolerance variety) and Situ Bagendit through marker-assisted backcrossing-through pyramiding gene of identified QTLs for foreground selection and to explore SSRs and 6K SNPs for background selection distributed in 12 rice chromosome of drought tolerant donor (Cabacu) and recipient rice (Inpari 30 and Situ Bagendit). The foreground selection revealed that flanking SSRs of each QTLs (qRPF2.1, qGPP2.1, qSPP4.1 and Sub1) was less than 2 cM. The background selection through polymorphic survey of Rice 6K SNP primers revealed 2457 (53,3%) polymorphic SNPs on Inpari 30 vs Cabacu and 2563 (55,6%) polymorphic SNPs on Situ Bagendit vs Cabacu with the average distance about 0.74 cM/chromosome. The genotypic selection of F1 Inpari 30/Cabacu and F1 Situ Bagendit/Cabacu have already in heterozygote condition for these 4 QTLs target. These lines was continued for backcross breeding to develop BC1F1 Inpari 30/Cabacu and BC1F1 Situ Bagendit/Cabacu generation.
国家稻米生产的可持续性和增加需要粮食和农业部门准备好应对气候变化、土地退化、干旱地区、倾斜生产和人口增长加快的影响。适应在确保粮食安全的可持续性方面发挥着重要作用。本研究旨在利用标记辅助回交技术,通过对已鉴定的前景选择qtl进行基因金字塔化,培育耐旱品种Inpari 30和Situ Bagendit,并探索分布在耐旱供体(Cabacu)和受体水稻(Inpari 30和Situ Bagendit) 12条水稻染色体上的SSRs和6K snp用于背景选择。前景选择结果显示,qRPF2.1、qGPP2.1、qSPP4.1和Sub1四个qtl的侧翼ssr均小于2 cM。通过水稻6K SNP引物多态性调查进行背景选择,Inpari 30与Cabacu的多态性位点为2457个(53.3%),原位Bagendit与Cabacu的多态性位点为2563个(55.6%),平均距离约为0.74 cM/染色体。F1 Inpari 30/Cabacu和F1 Situ Bagendit/Cabacu这4个qtl的基因型选择均已达到杂合子条件。将这些系进行回交育种,获得BC1F1 Inpari 30/Cabacu代和BC1F1 Situ Bagendit/Cabacu代。
{"title":"Molecular Evaluation for Drought Tolerant Using Marker Assisted Breeding Method","authors":"F. Fatimah, J. Prasetiyono, K. Trijatmiko, Sustiprijatno Sustiprijatno","doi":"10.14203/ann.bogor.2018.v22.n2.94-100","DOIUrl":"https://doi.org/10.14203/ann.bogor.2018.v22.n2.94-100","url":null,"abstract":"   The sustainability and increasing the national rice production require the readiness of food and agriculture sector cope with the impacts of climate change, land degradation, drought area, sloping production and the raising of population growth. Adaptation plays an important role in ensuring the sustainability of food security. This research aimed to develop drought-tolerant variety of Inpari 30 (submergence tolerance variety) and Situ Bagendit through marker-assisted backcrossing-through pyramiding gene of identified QTLs for foreground selection and to explore SSRs and 6K SNPs for background selection distributed in 12 rice chromosome of drought tolerant donor (Cabacu) and recipient rice (Inpari 30 and Situ Bagendit). The foreground selection revealed that flanking SSRs of each QTLs (qRPF2.1, qGPP2.1, qSPP4.1 and Sub1) was less than 2 cM. The background selection through polymorphic survey of Rice 6K SNP primers revealed 2457 (53,3%) polymorphic SNPs on Inpari 30 vs Cabacu and 2563 (55,6%) polymorphic SNPs on Situ Bagendit vs Cabacu with the average distance about 0.74 cM/chromosome. The genotypic selection of F1 Inpari 30/Cabacu and F1 Situ Bagendit/Cabacu have already in heterozygote condition for these 4 QTLs target. These lines was continued for backcross breeding to develop BC1F1 Inpari 30/Cabacu and BC1F1 Situ Bagendit/Cabacu generation.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82190745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Medium Chain and Long Chain Alkanes Hydroxylase Producing Whole Cell Biocatalyst From Marine Bacteria 海洋细菌中链和长链烷烃羟化酶生产全细胞生物催化剂
Pub Date : 2018-06-29 DOI: 10.14203/ANN.BOGOR.2018.V22.N1.12-19
A. Thontowi, E. Yetti, Yopi Yopi
Alkanes are  major component of crude oil that could be hydrolyzed by the enzyme of alkane hydroxylase. The are three types of alkane hydroxylase based on the chain length of alkane such as short-chain length/SCL (C2-C4), medium-chain length/MCL (C5-C17), and long-chain length/LCL (C>18). The aims of this study were to characterize and identify alkanes-degrading bacteria from these bacteria. The 30 strains from marine were grown on MCL (Pentane-C5H12, Decane-C10H22, and Pentadecane-C15H32) and LCL (n-Paraffin-C12H19C17 and branch of Pristane-C19H40). The study showed twenty-nine isolates have the ability to degrade alkanes compounds, whereas 14 isolates have grown ability on MCL and LCL medium, 11 isolates have the ability to grow on MCL and n-LCL, 3 isolates have the ability only to grow on MCL medium and 1 isolate has the ability only grow on n-LCL medium. The growth test result indicated that 29 isolates have medium-chain alkane monooxygenase and long-chain alkane hydroxylase. Based on 16S rDNA gene analysis, we obtained twenty nine of oil- degrading bacteria, namely a-proteobacteria (57 %), g-proteobacteria (30 %), Flavobacteria (7 %), Bacilli (3%) and Propionibacteriales (3 %). g-Proteobacteria and a-proteobacteria which seems to play an important role in the alkane biodegradation.
烷烃是原油的主要成分,可被烷烃羟化酶水解。烷烃羟化酶根据烷烃的链长分为短链长/SCL (C2-C4)、中链长/MCL (C5-C17)和长链长/LCL (C>18)三类。本研究的目的是表征和鉴定这些细菌中的烷烃降解菌。在MCL (Pentane-C5H12、Decane-C10H22和Pentadecane-C15H32)和LCL (n-Paraffin-C12H19C17和Pristane-C19H40分支)上培养30株海洋菌株。研究表明,29株菌株具有降解烷烃化合物的能力,14株菌株在MCL和LCL培养基上生长,11株菌株在MCL和n-LCL培养基上生长,3株菌株仅在MCL培养基上生长,1株菌株仅在n-LCL培养基上生长。生长试验结果表明,29株分离株具有中链烷烃单加氧酶和长链烷烃羟化酶。通过16S rDNA基因分析,共获得29种油降解菌,分别为a-变形菌属(57%)、g-变形菌属(30%)、Flavobacteria(7%)、Bacilli(3%)和Propionibacteriales(3%)。g-变形菌和a-变形菌似乎在烷烃生物降解中起重要作用。
{"title":"Medium Chain and Long Chain Alkanes Hydroxylase Producing Whole Cell Biocatalyst From Marine Bacteria","authors":"A. Thontowi, E. Yetti, Yopi Yopi","doi":"10.14203/ANN.BOGOR.2018.V22.N1.12-19","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2018.V22.N1.12-19","url":null,"abstract":"Alkanes are  major component of crude oil that could be hydrolyzed by the enzyme of alkane hydroxylase. The are three types of alkane hydroxylase based on the chain length of alkane such as short-chain length/SCL (C2-C4), medium-chain length/MCL (C5-C17), and long-chain length/LCL (C>18). The aims of this study were to characterize and identify alkanes-degrading bacteria from these bacteria. The 30 strains from marine were grown on MCL (Pentane-C5H12, Decane-C10H22, and Pentadecane-C15H32) and LCL (n-Paraffin-C12H19C17 and branch of Pristane-C19H40). The study showed twenty-nine isolates have the ability to degrade alkanes compounds, whereas 14 isolates have grown ability on MCL and LCL medium, 11 isolates have the ability to grow on MCL and n-LCL, 3 isolates have the ability only to grow on MCL medium and 1 isolate has the ability only grow on n-LCL medium. The growth test result indicated that 29 isolates have medium-chain alkane monooxygenase and long-chain alkane hydroxylase. Based on 16S rDNA gene analysis, we obtained twenty nine of oil- degrading bacteria, namely a-proteobacteria (57 %), g-proteobacteria (30 %), Flavobacteria (7 %), Bacilli (3%) and Propionibacteriales (3 %). g-Proteobacteria and a-proteobacteria which seems to play an important role in the alkane biodegradation.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86259526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Molecular Detection of Resistance To Bacterial Leaf Blight on Conde Indonesian Rice Variety 印尼Conde水稻品种对白叶枯病抗性的分子检测
Pub Date : 2018-06-29 DOI: 10.14203/ANN.BOGOR.2018.V22.N1.27-34
F. Fatimah, J. Prasetiyono, A. Polosoro, M. Baroya
Rice bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo) affected grain yield and decreasing rice production in rice growing countries. Conde, Indonesian rice variety, exhibits high resistance to most of the Indonesian races of (BLB) and has been used in Indonesia for cultivated rice. This study was aimed to conduct the molecular detection between proximal markers in chromosome 6 and relative expression of Conde rice variety compare to IRBB7 in Xa7 region. The population screening, BLB evaluation and molecular detection around the Xa7 region were conducted. The results showed from the collection of individual recombinants between resistant and susceptible parents narrow the region containing the BTBPOZ domain. The sequence alignment of Xa7LD37 in two resistant and three susceptible cultivars demonstrated a perfect association. The sequence alignment in exon region of Loc_Os06g46240 in Nipponbare, IRBB7, and IR64 identified indel/SNPs in this region leading to nucleotide substitution and frameshift resulting in amino acid change between resistant and susceptible cultivars. It was predicted that Conde revealed the similar gene action with Xa7 gene for BLB that encodes a BTB POZ domain.
水稻黄单胞菌引起的水稻细菌性叶枯病(BLB)。稻瘟病(Xoo)对水稻种植国的粮食产量造成影响,导致水稻产量下降。Conde是印度尼西亚的一个水稻品种,对大多数印度尼西亚的BLB具有很高的抗性,已在印度尼西亚用于栽培水稻。本研究旨在对Conde水稻品种6号染色体近端标记与IRBB7在Xa7区域的相对表达量进行分子检测。对Xa7区域进行种群筛选、BLB评价和分子检测。结果表明,抗性亲本和易感亲本之间的单个重组体缩小了含有BTBPOZ结构域的区域。Xa7LD37在2个抗性品种和3个敏感品种的序列比对显示出良好的关联。在Nipponbare、IRBB7和IR64的Loc_Os06g46240的外显子区域进行序列比对,发现该区域存在indel/SNPs,导致核苷酸置换和移码导致抗性和易感品种之间的氨基酸变化。据预测,Conde揭示了编码BTB POZ结构域的BLB基因与Xa7基因相似的基因作用。
{"title":"Molecular Detection of Resistance To Bacterial Leaf Blight on Conde Indonesian Rice Variety","authors":"F. Fatimah, J. Prasetiyono, A. Polosoro, M. Baroya","doi":"10.14203/ANN.BOGOR.2018.V22.N1.27-34","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2018.V22.N1.27-34","url":null,"abstract":"Rice bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo) affected grain yield and decreasing rice production in rice growing countries. Conde, Indonesian rice variety, exhibits high resistance to most of the Indonesian races of (BLB) and has been used in Indonesia for cultivated rice. This study was aimed to conduct the molecular detection between proximal markers in chromosome 6 and relative expression of Conde rice variety compare to IRBB7 in Xa7 region. The population screening, BLB evaluation and molecular detection around the Xa7 region were conducted. The results showed from the collection of individual recombinants between resistant and susceptible parents narrow the region containing the BTBPOZ domain. The sequence alignment of Xa7LD37 in two resistant and three susceptible cultivars demonstrated a perfect association. The sequence alignment in exon region of Loc_Os06g46240 in Nipponbare, IRBB7, and IR64 identified indel/SNPs in this region leading to nucleotide substitution and frameshift resulting in amino acid change between resistant and susceptible cultivars. It was predicted that Conde revealed the similar gene action with Xa7 gene for BLB that encodes a BTB POZ domain.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73841420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Growth and Proline Content of Irradiated in Vitro Shoots of Ubi Kuning Cassava Genotype Cultured at Different Temperatures 不同温度下育比昆宁木薯基因型离体苗的生长和脯氨酸含量
Pub Date : 2018-06-29 DOI: 10.14203/ANN.BOGOR.2018.V22.N1.35-46
Supatmi Supatmi, N. Rahman, N. Hartati
Cassava (Manihot esculenta) is an important crop to food security under climate change due to its various tolerance mechanism under stress conditions. However, the sustainable growth of cassava in the field depends on many factors especially temperature. The objective of the research was to investigate the growth performances and proline contents of irradiated Ubi Kuning at dosage of 10 Gy, cultured in Murashige Skoog (MS) hormone-free solid medium for 4 weeks at three different temperature treatments i.e 25oC, 28oC and 30oC. Each treatment consisted of 3 clone explants with 5 replicates. Results show that growth performances of irradiated plantlets were better compared to that of non-irradiated plantlets in terms of plant height and number of leaves at all temperature tested. The best growth performances were obtained from irradiated plantlets grown under 30oC. The proline content of irradiated Ubi Kuning was high when they were grown under 25 oC and 30oC, implying that these plantlets had the possibility to tolerant to lower and higher-temperature condition. This study is initially useful to find out the growth ability of irradiated Ubi Kuning in response to lower and higher temperature.
木薯在逆境条件下具有不同的耐受机制,是气候变化条件下保障粮食安全的重要作物。然而,木薯在田间的可持续生长取决于许多因素,尤其是温度。本试验旨在研究10 Gy辐照后的乌比库宁在无激素固体培养基中25℃、28℃和30℃3种不同温度下培养4周后的生长性能和脯氨酸含量。每个处理为3个无性系外植体,5个重复。结果表明,在不同温度下,辐照苗的株高和叶片数均优于未辐照苗。在30℃下生长的幼苗生长性能最好。在25℃和30℃条件下,辐照后的乌壁昆宁植株脯氨酸含量较高,具有耐低温和耐高温的可能性。本研究对了解辐照后乌壁库宁对低温和高温的生长能力有初步的指导意义。
{"title":"Growth and Proline Content of Irradiated in Vitro Shoots of Ubi Kuning Cassava Genotype Cultured at Different Temperatures","authors":"Supatmi Supatmi, N. Rahman, N. Hartati","doi":"10.14203/ANN.BOGOR.2018.V22.N1.35-46","DOIUrl":"https://doi.org/10.14203/ANN.BOGOR.2018.V22.N1.35-46","url":null,"abstract":"Cassava (Manihot esculenta) is an important crop to food security under climate change due to its various tolerance mechanism under stress conditions. However, the sustainable growth of cassava in the field depends on many factors especially temperature. The objective of the research was to investigate the growth performances and proline contents of irradiated Ubi Kuning at dosage of 10 Gy, cultured in Murashige Skoog (MS) hormone-free solid medium for 4 weeks at three different temperature treatments i.e 25oC, 28oC and 30oC. Each treatment consisted of 3 clone explants with 5 replicates. Results show that growth performances of irradiated plantlets were better compared to that of non-irradiated plantlets in terms of plant height and number of leaves at all temperature tested. The best growth performances were obtained from irradiated plantlets grown under 30oC. The proline content of irradiated Ubi Kuning was high when they were grown under 25 oC and 30oC, implying that these plantlets had the possibility to tolerant to lower and higher-temperature condition. This study is initially useful to find out the growth ability of irradiated Ubi Kuning in response to lower and higher temperature.","PeriodicalId":41037,"journal":{"name":"Annales Bogorienses-Journal of Tropical General Botany","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75247542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Annales Bogorienses-Journal of Tropical General Botany
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1