Pub Date : 2019-09-30DOI: 10.1201/9780429491269-11
B. Lasseter
{"title":"In Silico Biochemistry The Evolution of Globins","authors":"B. Lasseter","doi":"10.1201/9780429491269-11","DOIUrl":"https://doi.org/10.1201/9780429491269-11","url":null,"abstract":"","PeriodicalId":413971,"journal":{"name":"Biochemistry in the Lab","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126534762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-30DOI: 10.1201/9781315384207-26
B. Lasseter
{"title":"Buffers","authors":"B. Lasseter","doi":"10.1201/9781315384207-26","DOIUrl":"https://doi.org/10.1201/9781315384207-26","url":null,"abstract":"","PeriodicalId":413971,"journal":{"name":"Biochemistry in the Lab","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132629574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Michaelis–Menten Kinetics","authors":"B. Lasseter","doi":"10.1201/9780429491269-8","DOIUrl":"https://doi.org/10.1201/9780429491269-8","url":null,"abstract":"","PeriodicalId":413971,"journal":{"name":"Biochemistry in the Lab","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129153541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A process used for separating mixtures by virtue of differences in absorbency. It involves stationary and mobile phases. The stationary phase was packed in a column with materials that can be of any partitioning capability, adsorption, ion exchange, or affinity. The mobile phase (either liquid or gas) is the mixture that required to be separated.
{"title":"Column Chromatography","authors":"B. Lasseter","doi":"10.1201/9780429491269-7","DOIUrl":"https://doi.org/10.1201/9780429491269-7","url":null,"abstract":"A process used for separating mixtures by virtue of differences in absorbency. It involves stationary and mobile phases. The stationary phase was packed in a column with materials that can be of any partitioning capability, adsorption, ion exchange, or affinity. The mobile phase (either liquid or gas) is the mixture that required to be separated.","PeriodicalId":413971,"journal":{"name":"Biochemistry in the Lab","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122730857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-30DOI: 10.1201/9780429491269-13
B. Lasseter
{"title":"Enzyme Inhibition","authors":"B. Lasseter","doi":"10.1201/9780429491269-13","DOIUrl":"https://doi.org/10.1201/9780429491269-13","url":null,"abstract":"","PeriodicalId":413971,"journal":{"name":"Biochemistry in the Lab","volume":"125 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124101546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-30DOI: 10.1201/9780429491269-15
B. Lasseter
{"title":"Fluorescence and Denaturation","authors":"B. Lasseter","doi":"10.1201/9780429491269-15","DOIUrl":"https://doi.org/10.1201/9780429491269-15","url":null,"abstract":"","PeriodicalId":413971,"journal":{"name":"Biochemistry in the Lab","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133226427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-30DOI: 10.1201/9780429491269-10
B. Lasseter
{"title":"Polyacrylamide Gels","authors":"B. Lasseter","doi":"10.1201/9780429491269-10","DOIUrl":"https://doi.org/10.1201/9780429491269-10","url":null,"abstract":"","PeriodicalId":413971,"journal":{"name":"Biochemistry in the Lab","volume":"134 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125474378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Discussion of Isoelectric Point and Effective Charge","authors":"B. Lasseter","doi":"10.1201/9780429491269-6","DOIUrl":"https://doi.org/10.1201/9780429491269-6","url":null,"abstract":"","PeriodicalId":413971,"journal":{"name":"Biochemistry in the Lab","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122066889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-30DOI: 10.1201/9780429491269-14
B. Lasseter
{"title":"Multisubstrate Kinetics","authors":"B. Lasseter","doi":"10.1201/9780429491269-14","DOIUrl":"https://doi.org/10.1201/9780429491269-14","url":null,"abstract":"","PeriodicalId":413971,"journal":{"name":"Biochemistry in the Lab","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126260947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Bio-Rad NGC Chromatography System is used for purification in discovery phases to identify lead candidates, development phases in which optimization and processes are established, and again when those processes are transferred to manufacturing. In a cGMP environment, effective sanitization procedures for small-batch production of biopharmaceuticals are required for regulated laboratories. Validating equipment cleanliness is critical in the pharmaceutical industry and of utmost importance to avoid contaminating drug products with microorganisms, viruses, endotoxins, cleaning solution residues, and the like. Contaminating an entire chromatography system with living organisms, followed by full-system decontamination, can verify how well the system can be cleaned and can help to identify problem areas.
{"title":"Protein Purification","authors":"B. Lasseter","doi":"10.1201/9780429491269-9","DOIUrl":"https://doi.org/10.1201/9780429491269-9","url":null,"abstract":"The Bio-Rad NGC Chromatography System is used for purification in discovery phases to identify lead candidates, development phases in which optimization and processes are established, and again when those processes are transferred to manufacturing. In a cGMP environment, effective sanitization procedures for small-batch production of biopharmaceuticals are required for regulated laboratories. Validating equipment cleanliness is critical in the pharmaceutical industry and of utmost importance to avoid contaminating drug products with microorganisms, viruses, endotoxins, cleaning solution residues, and the like. Contaminating an entire chromatography system with living organisms, followed by full-system decontamination, can verify how well the system can be cleaned and can help to identify problem areas.","PeriodicalId":413971,"journal":{"name":"Biochemistry in the Lab","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122342542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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