Pub Date : 2019-12-18DOI: 10.35503/ijmlr.2019.4303
Leicy Jazmín Ruiz Barrera, A. Chávez, H. Zerón
Introduction: To test the surface fixation method contrasting urine samples of women with GDM vs healthy pregnant women. Methods: This was a pilot descriptive study. Three groups were conformed: A) Pregnant women with GDM, B) Women with healthy pregnancies and C) Non-pregnant healthy women. The positiveness of the surface fixation method was contrasted with Odds Ratio. Results: 12 women with GDM, 14 with healthy pregnancies and 9 non-pregnant women were included in the study.The OR for a positive surface fixation test when contrasting GDM vs Healthy pregnancies was of 2.7 while the value when contrasting GDM vs Healthy pregnancies + Non pregnant women was of 3.2 without reaching significant statistical difference in any case. Conclusion: the surface fixation method used with urine samples, suggests the existence of a transient antigen-antibody reaction that contributes to the inefficient insulin secretion.
{"title":"TESTING THE SURFACE FIXATION METHOD IN GESTATIONAL DIABETES MELLITUS","authors":"Leicy Jazmín Ruiz Barrera, A. Chávez, H. Zerón","doi":"10.35503/ijmlr.2019.4303","DOIUrl":"https://doi.org/10.35503/ijmlr.2019.4303","url":null,"abstract":"Introduction: To test the surface fixation method contrasting urine samples of women with GDM vs healthy pregnant women. Methods: This was a pilot descriptive study. Three groups were conformed: A) Pregnant women with GDM, B) Women with healthy pregnancies and C) Non-pregnant healthy women. The positiveness of the surface fixation method was contrasted with Odds Ratio. Results: 12 women with GDM, 14 with healthy pregnancies and 9 non-pregnant women were included in the study.The OR for a positive surface fixation test when contrasting GDM vs Healthy pregnancies was of 2.7 while the value when contrasting GDM vs Healthy pregnancies + Non pregnant women was of 3.2 without reaching significant statistical difference in any case. Conclusion: the surface fixation method used with urine samples, suggests the existence of a transient antigen-antibody reaction that contributes to the inefficient insulin secretion.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123617812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.35503/ijmlr.2021.6209
S. Varsha, Shubha Hv, Vivek Tg, C. Vijaya
: Lipid disorders can occur either as primary event or secondary to an underlying disease. Primary dyslipidemias are associated with overproduction or impaired removal of lipoprotein. An abnormality in the lipoprotein itself or in the lipoprotein receptor induces impaired removal of lipoprotein. Hypertriglyceridemia is defined as having plasma triglyceride above the 95 th percentile for age and sex. It is a rare disorder in childhood. We present a case of six months old male infant who was admitted in our hospital with the history of anemia. Infant was detected to have highly viscous and milky serum during the sampling of blood coincidentally. The serum triglyceride and cholesterol levels were markedly raised. There were no typical signs of hyperlipidemia like growth failure, dysmorphic features, hepatosplenomegaly, eruptive cutaneous xanthomas or lipemia retinalis. There was also no positive family history of hyperlipidemia. A clinical diagnosis of Type I hypertriglyceridemia was made and the infant was put on lipid lowering drugs and iron drops. Infant responded well to the treatment. Hemoglobin levels improved with the improving lipid profile parameters. Early diagnosis and intervention can therefore, improve the prognosis by reducing the complications and preventing the premature death.
{"title":"AN INFANT PRESENTING WITH MILKY SERUM: A RARE CASE REPORT","authors":"S. Varsha, Shubha Hv, Vivek Tg, C. Vijaya","doi":"10.35503/ijmlr.2021.6209","DOIUrl":"https://doi.org/10.35503/ijmlr.2021.6209","url":null,"abstract":": Lipid disorders can occur either as primary event or secondary to an underlying disease. Primary dyslipidemias are associated with overproduction or impaired removal of lipoprotein. An abnormality in the lipoprotein itself or in the lipoprotein receptor induces impaired removal of lipoprotein. Hypertriglyceridemia is defined as having plasma triglyceride above the 95 th percentile for age and sex. It is a rare disorder in childhood. We present a case of six months old male infant who was admitted in our hospital with the history of anemia. Infant was detected to have highly viscous and milky serum during the sampling of blood coincidentally. The serum triglyceride and cholesterol levels were markedly raised. There were no typical signs of hyperlipidemia like growth failure, dysmorphic features, hepatosplenomegaly, eruptive cutaneous xanthomas or lipemia retinalis. There was also no positive family history of hyperlipidemia. A clinical diagnosis of Type I hypertriglyceridemia was made and the infant was put on lipid lowering drugs and iron drops. Infant responded well to the treatment. Hemoglobin levels improved with the improving lipid profile parameters. Early diagnosis and intervention can therefore, improve the prognosis by reducing the complications and preventing the premature death.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121796143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.35503/ijmlr.2022.7102
S. Rukwal, H. Kaur, P. Kaur, M. Shrivastava
BACKGROUND AND OBJECTIVES: Elevated Troponin-I levels either due to direct or indirect cardiac injury can present as asymptomatic to fulminant myocarditis and circulatory shock in COVID-19 patients. Our objective is to evaluate the Troponin-I levels in covid patients. AIM: To evaluate the possible myocardial involvement in covid-19 patients with the help of Troponin-I levels as an indicator of cardiac injury and its prognostic implication and significance as it is associated with increased mortality in patients with COVID-19. METHORDS: This was a both a prospective and retrospective cohort study done from 1st January 2021 to 1st July 2021. A total of 240 admitted patients were included and whose Troponin-I levels were evaluated and in a comparative analysis was performed for the demography, clinical features, biochemical markers and their outcome with their Troponin-I levels. RESULTS: Out of 250 covid-19 positive hospitalized patients, 15patients have elevated Troponin-I levels. Among these 15 patients 60% had preexisting co morbidities like hypertension, diabetes and history of cardiac disease. Patients with elevated troponin level had mean age of 61.1years, length of stay was >14 days in 60% patients. In 46% patients Troponin-I levels were more than 200ng/L. These patients had a higher need for intensive care with mortality of 73%. CONCLUSION: Etiology of myocardial involvement is multifactorial. Our study also concluded that elevated troponin levels in patients with covid19 have high morality. Therefore, the measurement of cardiac biomarkers, including Troponin-I and brain natriuretic peptide (BNP), should be performed on admission.
{"title":"STUDY OF TROPONIN-I LEVELS IN COVID-19 PATIENTS","authors":"S. Rukwal, H. Kaur, P. Kaur, M. Shrivastava","doi":"10.35503/ijmlr.2022.7102","DOIUrl":"https://doi.org/10.35503/ijmlr.2022.7102","url":null,"abstract":"BACKGROUND AND OBJECTIVES: Elevated Troponin-I levels either due to direct or indirect cardiac injury can present as asymptomatic to fulminant myocarditis and circulatory shock in COVID-19 patients. Our objective is to evaluate the Troponin-I levels in covid patients. AIM: To evaluate the possible myocardial involvement in covid-19 patients with the help of Troponin-I levels as an indicator of cardiac injury and its prognostic implication and significance as it is associated with increased mortality in patients with COVID-19. METHORDS: This was a both a prospective and retrospective cohort study done from 1st January 2021 to 1st July 2021. A total of 240 admitted patients were included and whose Troponin-I levels were evaluated and in a comparative analysis was performed for the demography, clinical features, biochemical markers and their outcome with their Troponin-I levels. RESULTS: Out of 250 covid-19 positive hospitalized patients, 15patients have elevated Troponin-I levels. Among these 15 patients 60% had preexisting co morbidities like hypertension, diabetes and history of cardiac disease. Patients with elevated troponin level had mean age of 61.1years, length of stay was >14 days in 60% patients. In 46% patients Troponin-I levels were more than 200ng/L. These patients had a higher need for intensive care with mortality of 73%. CONCLUSION: Etiology of myocardial involvement is multifactorial. Our study also concluded that elevated troponin levels in patients with covid19 have high morality. Therefore, the measurement of cardiac biomarkers, including Troponin-I and brain natriuretic peptide (BNP), should be performed on admission.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117259244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.35503/ijmlr.2020.5209
H. Shubha, A. Shetty, C. Vijaya, Venkatachalapathy
: Intestinal duplication cysts are rare congenital anomalies that are usually detected during infancy or early childhood. Though they can occur anywhere in the gastrointestinal tract, small intestine is the commonest site of occurrence. Majority of the enteric cysts have a communication with the adjacent bowel Completely isolated enteric duplication cysts are a rare variety of duplication cysts having no communication with any part of the adjacent bowel segment and an independent blood supply. These cysts often present with non- specific symptoms like abdominal pain and vomiting thereby ,highlighting the importance of ultrasound as an aid to precise and timely diagnosis. Histopathological examination is confirmatory, with excision of the cyst being the preferred treatment. We present a rare case of an isolated intestinal duplication cyst in a teenage boy which remained undetected in childhood.
{"title":"RARE CASE OF AN ISOLATED ENTERIC DUPLICATION CYST IN A TEENAGER","authors":"H. Shubha, A. Shetty, C. Vijaya, Venkatachalapathy","doi":"10.35503/ijmlr.2020.5209","DOIUrl":"https://doi.org/10.35503/ijmlr.2020.5209","url":null,"abstract":": Intestinal duplication cysts are rare congenital anomalies that are usually detected during infancy or early childhood. Though they can occur anywhere in the gastrointestinal tract, small intestine is the commonest site of occurrence. Majority of the enteric cysts have a communication with the adjacent bowel Completely isolated enteric duplication cysts are a rare variety of duplication cysts having no communication with any part of the adjacent bowel segment and an independent blood supply. These cysts often present with non- specific symptoms like abdominal pain and vomiting thereby ,highlighting the importance of ultrasound as an aid to precise and timely diagnosis. Histopathological examination is confirmatory, with excision of the cyst being the preferred treatment. We present a rare case of an isolated intestinal duplication cyst in a teenage boy which remained undetected in childhood.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128745276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.35503/ijmlr.2023.8104
Deepa Devhare
Vaginitis is a very common and recurrent infection in reproductive age group especially worrisome in pregnant females. If it is misdiagnosed, left untreated or wrongly treated can lead to serious complications. With this background present study was conducted to determine the prevalence, bacteriological profile and antimicrobial susceptibility of the pathogens causing aerobic vaginitis. Study was conducted over a period of 1year in which 241 vaginal swabs were processed for aerobic culture and gram stain. The organisms were identified and antimicrobial susceptibility was performed by Vitek 2 compact system. Out of 241 samples processed 37(15.4%) showed bacterial growth. S. aureus (40.5%), GBS (13.5%), E. coli (13.5%) and K. pneumoniae (13.5%) were the common pathogens isolated. Gram positive organisms showed highest sensitivity to Vancomycin and Linezolid. All gramnegative bacteria were sensitive to Imipenem and Meropenem. This study recommends the laboratory-based diagnosis of each clinically suspected case of vaginitis for appropriate management of patient. Addition of antibacterial agent in traditional empirical antifungal therapy for vaginitis is need of hour
{"title":"AEROBIC VAGINITIS (AV): DISEASE BURDEN, BACTERIOLOGICAL PROFILE AND ANTIMICROBIAL SUSCEPTIBILITY PATTERN IN WESTERN MAHARASHTRA","authors":"Deepa Devhare","doi":"10.35503/ijmlr.2023.8104","DOIUrl":"https://doi.org/10.35503/ijmlr.2023.8104","url":null,"abstract":"Vaginitis is a very common and recurrent infection in reproductive age group especially worrisome in pregnant females. If it is misdiagnosed, left untreated or wrongly treated can lead to serious complications. With this background present study was conducted to determine the prevalence, bacteriological profile and antimicrobial susceptibility of the pathogens causing aerobic vaginitis. Study was conducted over a period of 1year in which 241 vaginal swabs were processed for aerobic culture and gram stain. The organisms were identified and antimicrobial susceptibility was performed by Vitek 2 compact system. Out of 241 samples processed 37(15.4%) showed bacterial growth. S. aureus (40.5%), GBS (13.5%), E. coli (13.5%) and K. pneumoniae (13.5%) were the common pathogens isolated. Gram positive organisms showed highest sensitivity to Vancomycin and Linezolid. All gramnegative bacteria were sensitive to Imipenem and Meropenem. This study recommends the laboratory-based diagnosis of each clinically suspected case of vaginitis for appropriate management of patient. Addition of antibacterial agent in traditional empirical antifungal therapy for vaginitis is need of hour","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"229 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116247342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.35503/ijmlr.2020.5103
O. Abbadi, A. H. Osman, Naglaa A. Ali
: Overview: Physical forces are known to affect biological structure and modulate the extracellular matrix. Collagen is the most abundant protein outside the cell, and it is clearly highlighted through Masson trichrome and van Geison ’ s stain. This research aimed to calculate the specific concentration of collagen within histology slides, after being subjected to sessions of intermittent negative pressure (INP), using Adobe® Photoshop® software and mice models. Objectives: To measure the collagen concentration in histology slides through Adobe® Photoshop®, using two types of stains: Masson trichrome, and van Gieson ’ s stain. Methods: Sixteen mice were put into sessions of INP ten minutes daily, for three weeks. Skin tissues were extracted from test and control sides. Stained slides were photographed and inserted in the Adobe® Photoshop® program to measure collagen concentration according to the specified calculated areas within the slides. Results: Masson ’ s trichrome slides gave a mean Collagen concentration of (58.4%) in the test slides, compared to the control which was (39.9%). In van Gieson stained slides, the collagen gave a mean concentration of (54.93%) in test slides and (41.32%) in the control. The difference was statistically insignificant in both staining. Conclusion: Applying INP to mice skin for three weeks did not significantly change the concentration of the collagen in the extracellular matrix. van Gieson stain.
{"title":"THE EFFECT OF INTERMITTENT NEGATIVE PRESSURE ON COLLAGEN CONCENTRATION IN MICE SKIN","authors":"O. Abbadi, A. H. Osman, Naglaa A. Ali","doi":"10.35503/ijmlr.2020.5103","DOIUrl":"https://doi.org/10.35503/ijmlr.2020.5103","url":null,"abstract":": Overview: Physical forces are known to affect biological structure and modulate the extracellular matrix. Collagen is the most abundant protein outside the cell, and it is clearly highlighted through Masson trichrome and van Geison ’ s stain. This research aimed to calculate the specific concentration of collagen within histology slides, after being subjected to sessions of intermittent negative pressure (INP), using Adobe® Photoshop® software and mice models. Objectives: To measure the collagen concentration in histology slides through Adobe® Photoshop®, using two types of stains: Masson trichrome, and van Gieson ’ s stain. Methods: Sixteen mice were put into sessions of INP ten minutes daily, for three weeks. Skin tissues were extracted from test and control sides. Stained slides were photographed and inserted in the Adobe® Photoshop® program to measure collagen concentration according to the specified calculated areas within the slides. Results: Masson ’ s trichrome slides gave a mean Collagen concentration of (58.4%) in the test slides, compared to the control which was (39.9%). In van Gieson stained slides, the collagen gave a mean concentration of (54.93%) in test slides and (41.32%) in the control. The difference was statistically insignificant in both staining. Conclusion: Applying INP to mice skin for three weeks did not significantly change the concentration of the collagen in the extracellular matrix. van Gieson stain.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127701942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.35503/ijmlr.2022.7103
F. Fazal, M. Pais, RG D’Silva, NM Geevarghese, S. Suresh, Mohan R Baliga, RP Jakribettu
Background: Presently, the COVID 19 has made its presence all over the world. Since, there is no definitive cure available, prevention is the only mode to control the increase of the disease. Thus, this study was undertaken to evaluate the knowledge and attitude of medical students towards COVID19 in Mangalore, Karnataka, India. Methods: An online questionnaire was circulated among the medical students using the Google form. The questionnaire contained questions regarding the demographic details, attitude and knowledge of the students to be assessed. The response was typed in Microsoft Excel and analyzed using the Statistical Package for Social Sciences (SPSS) version 17 and data represented as frequency (n) and percentage (%). Results: A total of 321 responses were received from medical students, majority were female (199, 62%), nearly 50% were from Final (Clinical) phase. More than 10% of the students believed that the pandemic will last for more than 1 year. Most of the students were aware of the methods to prevent COVID 19 while most were unsure whether antibiotics (76%), hydroxychloroquine (47%), herbal drugs (97%) useful in prevention of COVID 19. Conclusion: The medical students were aware of the pathogenesis of COVID and IPC practices to be followed to prevent its spread. But they were not sure about the appropriate medication for the disease, which was not clear even to specialist, during the period which the study was undertaken.
{"title":"KNOWLEDGE AND ATTITUDES OF MEDICAL STUDENTS TOWARDS COVID-19: A CROSS-SECTIONAL STUDY DURING THE EARLY STAGES OF PANDEMIC IN INDIA","authors":"F. Fazal, M. Pais, RG D’Silva, NM Geevarghese, S. Suresh, Mohan R Baliga, RP Jakribettu","doi":"10.35503/ijmlr.2022.7103","DOIUrl":"https://doi.org/10.35503/ijmlr.2022.7103","url":null,"abstract":"Background: Presently, the COVID 19 has made its presence all over the world. Since, there is no definitive cure available, prevention is the only mode to control the increase of the disease. Thus, this study was undertaken to evaluate the knowledge and attitude of medical students towards COVID19 in Mangalore, Karnataka, India. Methods: An online questionnaire was circulated among the medical students using the Google form. The questionnaire contained questions regarding the demographic details, attitude and knowledge of the students to be assessed. The response was typed in Microsoft Excel and analyzed using the Statistical Package for Social Sciences (SPSS) version 17 and data represented as frequency (n) and percentage (%). Results: A total of 321 responses were received from medical students, majority were female (199, 62%), nearly 50% were from Final (Clinical) phase. More than 10% of the students believed that the pandemic will last for more than 1 year. Most of the students were aware of the methods to prevent COVID 19 while most were unsure whether antibiotics (76%), hydroxychloroquine (47%), herbal drugs (97%) useful in prevention of COVID 19. Conclusion: The medical students were aware of the pathogenesis of COVID and IPC practices to be followed to prevent its spread. But they were not sure about the appropriate medication for the disease, which was not clear even to specialist, during the period which the study was undertaken.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134620760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.35503/ijmlr.2022.7201
M. H. I. Mustafa, Tariq E. Elmissbah, E. Khalil
Background. This is a community-based, prospective, and analytical study aimed to determine the frequencies of Rhesus blood group (Rh) alleles, haplotypes, and probable genotypes, and establishing baseline data. Materials and Methods. Following informed consent, a total of 1,000 venous blood samples from unrelated individuals were collected. Particle gel immunological diffusion and slide agglutination procedures were used to screen red blood cells for common Rhesus antigens. The frequencies of genes/alleles and haplotypes, as well as the most likely genotypes, were determined. Jaccard's coefficient of similarities was used to calculate similarities between different Sudanese populations. The same coefficient was used to figure out where these people came from. Results. The ē, and the D genes were the commonest alleles detected with frequencies of 98.4%, 93.8% and 90.7% respectively. The C and the E genes were the least frequent with 58.4% and 21.0% frequencies respectively. The Commonest Rh genotype encountered was the (frequency= 44.2%). The and genotypes were detected with lower frequencies of 21.7% and 10.9% respectively, while , and genotypes were the least common [frequencies 0.3%), 0.2% and 0.1% respectively]. The most probably common haplotypes complex was / (frequency= 44.2%) and the least common was / (frequency= 0.1%). Conclusion- The antigen e was found to be the most frequent in major Sudanese populations, when the frequency of E antigen was the least once. the frequency of c antigen among this study populations exhibited high frequency (99).
{"title":"FREQUENCIES OF RHESUS BLOOD GROUP SUBTYPES, AND PROBABLE GENOTYPES IN SUDANESE POPULATION","authors":"M. H. I. Mustafa, Tariq E. Elmissbah, E. Khalil","doi":"10.35503/ijmlr.2022.7201","DOIUrl":"https://doi.org/10.35503/ijmlr.2022.7201","url":null,"abstract":"Background. This is a community-based, prospective, and analytical study aimed to determine the frequencies of Rhesus blood group (Rh) alleles, haplotypes, and probable genotypes, and establishing baseline data. Materials and Methods. Following informed consent, a total of 1,000 venous blood samples from unrelated individuals were collected. Particle gel immunological diffusion and slide agglutination procedures were used to screen red blood cells for common Rhesus antigens. The frequencies of genes/alleles and haplotypes, as well as the most likely genotypes, were determined. Jaccard's coefficient of similarities was used to calculate similarities between different Sudanese populations. The same coefficient was used to figure out where these people came from. Results. The ē, and the D genes were the commonest alleles detected with frequencies of 98.4%, 93.8% and 90.7% respectively. The C and the E genes were the least frequent with 58.4% and 21.0% frequencies respectively. The Commonest Rh genotype encountered was the (frequency= 44.2%). The and genotypes were detected with lower frequencies of 21.7% and 10.9% respectively, while , and genotypes were the least common [frequencies 0.3%), 0.2% and 0.1% respectively]. The most probably common haplotypes complex was / (frequency= 44.2%) and the least common was / (frequency= 0.1%). Conclusion- The antigen e was found to be the most frequent in major Sudanese populations, when the frequency of E antigen was the least once. the frequency of c antigen among this study populations exhibited high frequency (99).","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124659922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.35503/ijmlr.2019.4203
E. Saldanha, A. Alva, RP Jakribettu, S. Sajan, P. Simon, N. Sequeira, F. Fazal, Baliga
Aim: To study the clinical profile with co-morbidities of patients admitted with Diabetic foot ulcer (DFU) infection. Also, to study the microbiological flora causing the infection, along with antibiotic susceptibility pattern of the pathogens isolated from these ulcers. Material and method: A prospective study was carried out on 95 diabetic patients with foot ulcers for a period of 11⁄2 years. The demographic details of the patients with Diabetic foot ulcer (DFU) along with Wagner’s grade and treatment profile were also collected. The tissue sample were collected and processed as per standard microbiological procedure and antibiotic susceptibility testing was done as per Clinical & Laboratory Standards Institute (CLSI). The data was analyzed by percentage and correlated. Results: A total of 95 patients were included in the study with 83 males. The majority of patients with DFU were below the age of 40 years. Around 36.48% patients developed DFU following 6-10 years of diagnosed as Diabetes mellitus. Hypertension (26.88%) and neuropathy (20%) were the most common co-morbidity and complications among these patients. A total of 92 pathogens isolated from these patients, with Gram negative pathogens being predominant. The most common pathogens isolated were Pseudomonas aeruginosa (30%), followed by Escherichia coli(28.8%) and Staphylococcus aureus (72.5%) among Gram negative and Gram positive pathogens, respectively. Minimal resistance was noted for high end antimicrobials like Beta lactamBeta lactamase inhibitors & carbapenems. But, for empirical therapy, fluoroquinoles and aminoglycosides may be considered as alternative for cephalosporins. Conclusion: Diabetic foot ulcer infection is mainly seen in individuals with > 5 years of diabetes and complications especially peripheral neuropathy and nephropathy. Gram negative bacteria, coliforms are predominant pathogens isolated. Flouroquinolones and aminoglycoside group of antimicrobials can be considered for empirical therapy provided antibiogram is monitored on regular basis.
{"title":"CLINICO-BACTERIOLOGICAL PROFILE IN DIABETICS ADMITTED FOR FOOT ULCERS: OBSERVATIONS FROM A TERTIARY CARE HOSPITAL","authors":"E. Saldanha, A. Alva, RP Jakribettu, S. Sajan, P. Simon, N. Sequeira, F. Fazal, Baliga","doi":"10.35503/ijmlr.2019.4203","DOIUrl":"https://doi.org/10.35503/ijmlr.2019.4203","url":null,"abstract":"Aim: To study the clinical profile with co-morbidities of patients admitted with Diabetic foot ulcer (DFU) infection. Also, to study the microbiological flora causing the infection, along with antibiotic susceptibility pattern of the pathogens isolated from these ulcers. Material and method: A prospective study was carried out on 95 diabetic patients with foot ulcers for a period of 11⁄2 years. The demographic details of the patients with Diabetic foot ulcer (DFU) along with Wagner’s grade and treatment profile were also collected. The tissue sample were collected and processed as per standard microbiological procedure and antibiotic susceptibility testing was done as per Clinical & Laboratory Standards Institute (CLSI). The data was analyzed by percentage and correlated. Results: A total of 95 patients were included in the study with 83 males. The majority of patients with DFU were below the age of 40 years. Around 36.48% patients developed DFU following 6-10 years of diagnosed as Diabetes mellitus. Hypertension (26.88%) and neuropathy (20%) were the most common co-morbidity and complications among these patients. A total of 92 pathogens isolated from these patients, with Gram negative pathogens being predominant. The most common pathogens isolated were Pseudomonas aeruginosa (30%), followed by Escherichia coli(28.8%) and Staphylococcus aureus (72.5%) among Gram negative and Gram positive pathogens, respectively. Minimal resistance was noted for high end antimicrobials like Beta lactamBeta lactamase inhibitors & carbapenems. But, for empirical therapy, fluoroquinoles and aminoglycosides may be considered as alternative for cephalosporins. Conclusion: Diabetic foot ulcer infection is mainly seen in individuals with > 5 years of diabetes and complications especially peripheral neuropathy and nephropathy. Gram negative bacteria, coliforms are predominant pathogens isolated. Flouroquinolones and aminoglycoside group of antimicrobials can be considered for empirical therapy provided antibiogram is monitored on regular basis.","PeriodicalId":420042,"journal":{"name":"International Journal of Medical Laboratory Research","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128248182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.35503/IJMLR.2019.0404
C. L. Singnap, A. Sabo, Oto-Obong Victor Idah, Bitrus N. Lekshak, T. Yakubu, Moses D. Lugo
Background: It is estimated that over 80% of people still depend mainly on the traditional use of parts of plants and herbs to treat ailments. The medicinal properties of these plants and herbs are linked to the presence of a variety of phytochemicals and their elemental composition. Justification: Every year, 1 in 4 people die of conditions related to thrombosis, with many never knowing their risk for the condition. Prevention of intravascular thrombosis, however, has a narrow therapeutic window, bleeding risk, the incidence of resistance, and unwanted drug interactions, hence the need for anti-thrombotic drugs that deliver more effective prevention of intravascular thrombosis. Aim: The research sought to investigate the effect of Moringaoleifera ethanolic seed extract on Bleeding Time (BT) and Clotting Time (CT), in Wistar albino rats. Materials and Methods: Forty (40)Wistar rats were weighed and randomly divided into two groups, group I=15 rats for BT, group II=15 rats for CT and 10 rats for control tests. After the administration of ethanolic extract of M. oleifera seed at the dose of 100mg/kg, 200mg/kg and 400mg/kg (administered to a group of 5 rats per dose) for 28 days, BT and CT were determined using Tail transection and Lee & White method respectively. The data were analysed using GraphPadPrism (7.03). Result: There was a statistically significant increase at P <0.0001 of BT and CT compared to the control (administered with only distilled water). Conclusion: The prolonged BT and CT indicate that the seed extract of M. oleifera could pose an antagonising effect on both the primary (platelets) and secondary haemostatic activities, a property that can be explored in the management of thrombotic diseases.
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