Pub Date : 2021-07-21DOI: 10.9734/BPI/CASTR/V12/11397D
O. N. Ewoti, D. E. Menye, R. P. T. Kayo, L. M. Moungang, A. T. Arfao, S. Baleng, Yves Yogne Poutoum, M. Nola
A study was carried out in an aquatic microcosm with the aim of evaluating the importance of some chemical factors of the environment and some parameters intrinsic to cells, on the retention of bacterial contaminants in the aquatic environment on solid substrates. The rocky substrates used were sandstone, basalt, granite and micaschist. Four rocks with different petrographic and mineralogical structures. Bacteria isolated by standard techniques and used for adhesion testing were Pseudomonas aeruginosa, Vibrio cholerae and Enterococcus faecalis. These bacteria are rod, comma, and spherical in shape, respectively, and may or may not have a flagellum. In addition, this ciliature, when it exists, can be single or in a tuft and in a polar position or over the entire cell. The chemical parameters of the medium which varied were the pH, the concentrations of BOM and of salts. The incubation periods to allow the bacteria to adhere to the substrates ranged from 180min to 1440min. The adhered cells were then detached in sterile condition by introducing the rocks successively into 3 test tubes containing 10 ml of sterile physiological water (0.85% NaCl). It appears that bacteria contained in surface or groundwater can adhere to the surfaces of inorganic rocks at varying concentrations. The adhesion and retention of bacteria contributes to the natural purification of wastewater. P. aeruginosa, V. cholerae and E. faecalis adhered undergo temporal variations. Adhesion rates overall vary from 11 to 12.44x103 cells/cm2/h, with E. faecalis being the bacteria which adheres more quickly due to gravity alone. These variations of rates are linked to environmental conditions and to the chemical properties of rocks and soil. The presence of flagellum plays a dual role in approaching and relaunching the bacteria from the substrates.
{"title":"Study on Adhesion of Bacterio-contaminants to Fragments of Rocks Immersed in Water: Impact of Some Chemical Properties of the Medium and Intrinsic to the Cells","authors":"O. N. Ewoti, D. E. Menye, R. P. T. Kayo, L. M. Moungang, A. T. Arfao, S. Baleng, Yves Yogne Poutoum, M. Nola","doi":"10.9734/BPI/CASTR/V12/11397D","DOIUrl":"https://doi.org/10.9734/BPI/CASTR/V12/11397D","url":null,"abstract":"A study was carried out in an aquatic microcosm with the aim of evaluating the importance of some chemical factors of the environment and some parameters intrinsic to cells, on the retention of bacterial contaminants in the aquatic environment on solid substrates. The rocky substrates used were sandstone, basalt, granite and micaschist. Four rocks with different petrographic and mineralogical structures. Bacteria isolated by standard techniques and used for adhesion testing were Pseudomonas aeruginosa, Vibrio cholerae and Enterococcus faecalis. These bacteria are rod, comma, and spherical in shape, respectively, and may or may not have a flagellum. In addition, this ciliature, when it exists, can be single or in a tuft and in a polar position or over the entire cell. The chemical parameters of the medium which varied were the pH, the concentrations of BOM and of salts. The incubation periods to allow the bacteria to adhere to the substrates ranged from 180min to 1440min. The adhered cells were then detached in sterile condition by introducing the rocks successively into 3 test tubes containing 10 ml of sterile physiological water (0.85% NaCl). It appears that bacteria contained in surface or groundwater can adhere to the surfaces of inorganic rocks at varying concentrations. The adhesion and retention of bacteria contributes to the natural purification of wastewater. P. aeruginosa, V. cholerae and E. faecalis adhered undergo temporal variations. Adhesion rates overall vary from 11 to 12.44x103 cells/cm2/h, with E. faecalis being the bacteria which adheres more quickly due to gravity alone. These variations of rates are linked to environmental conditions and to the chemical properties of rocks and soil. The presence of flagellum plays a dual role in approaching and relaunching the bacteria from the substrates.","PeriodicalId":437958,"journal":{"name":"Current Approaches in Science and Technology Research Vol. 12","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121616675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-21DOI: 10.9734/BPI/CASTR/V12/10370D
O. Diakite, Mamadou Aissata, Aliou Sissoko, Sekouba Sanogo, M. Kouressy, M. Vaksmann, D. Dzidzienyo, E. Danquah, T. Pangirayi, K. Traore, N. Témé
Introgression of bmr genes from less adapted donor parent to well adapted high yielding biomass varieties with poor nutritional value is very important for sustainable cattle feeding during pasture scare time in the Sahel. The main objective of this work was to introgress bmr6 and bmr12 genes in Nigerien and Malian sorghum varieties background for dual purpose grain and biomass potential. The plant material was composed of two improved sorghum varieties (Sepon82 and Kalla Kene) and El mota a farmer preferred variety as recurrent parents. bmr donor parents were redlan bmr6, Tx630 bmr12 and Wheatland bmr12. The hand emasculation technique was used to introgress bmr genes in recurrent parents to produce F3 and BC1F3 populations at Sotuba research Station in Mali from January 2016 to June 2017. Anthocyanin pigment and heterosis effects were key phenotypic traits to identify F1 and BC1F1 plants during the population development. Anthocyanin allowed the identification of F1 plants in a cross involving anthocyanin (purple plant) and tan plants, while for both tan plants cross, heterosis effect was major key to discriminate F1 from parental lines and bmr segregation in F2 to ascertain successful crosses. The χ2 test was used to analyze bmr segregation ration. Segregation ratios of bmr plants in F2 and BC1F2 showed a good fit of a single recessive gene (3:1). bmr 6 and 12 genes were successfully transferred to three recurrent parents varieties which are at F4 and BC1F3 generation for grain and biomass yields potential tests in Niger during the 2017 cropping season.
{"title":"Studies on Brown Midrib 6 and 12 Genes Introgression in Two Nigerien and One Malian Sorghum Varieties: A Practical Guide to Young Scientists with Limited Molecular Facility","authors":"O. Diakite, Mamadou Aissata, Aliou Sissoko, Sekouba Sanogo, M. Kouressy, M. Vaksmann, D. Dzidzienyo, E. Danquah, T. Pangirayi, K. Traore, N. Témé","doi":"10.9734/BPI/CASTR/V12/10370D","DOIUrl":"https://doi.org/10.9734/BPI/CASTR/V12/10370D","url":null,"abstract":"Introgression of bmr genes from less adapted donor parent to well adapted high yielding biomass varieties with poor nutritional value is very important for sustainable cattle feeding during pasture scare time in the Sahel. The main objective of this work was to introgress bmr6 and bmr12 genes in Nigerien and Malian sorghum varieties background for dual purpose grain and biomass potential. The plant material was composed of two improved sorghum varieties (Sepon82 and Kalla Kene) and El mota a farmer preferred variety as recurrent parents. bmr donor parents were redlan bmr6, Tx630 bmr12 and Wheatland bmr12. The hand emasculation technique was used to introgress bmr genes in recurrent parents to produce F3 and BC1F3 populations at Sotuba research Station in Mali from January 2016 to June 2017. Anthocyanin pigment and heterosis effects were key phenotypic traits to identify F1 and BC1F1 plants during the population development. Anthocyanin allowed the identification of F1 plants in a cross involving anthocyanin (purple plant) and tan plants, while for both tan plants cross, heterosis effect was major key to discriminate F1 from parental lines and bmr segregation in F2 to ascertain successful crosses. The χ2 test was used to analyze bmr segregation ration. Segregation ratios of bmr plants in F2 and BC1F2 showed a good fit of a single recessive gene (3:1). bmr 6 and 12 genes were successfully transferred to three recurrent parents varieties which are at F4 and BC1F3 generation for grain and biomass yields potential tests in Niger during the 2017 cropping season.","PeriodicalId":437958,"journal":{"name":"Current Approaches in Science and Technology Research Vol. 12","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133138470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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