Pub Date : 2020-07-01DOI: 10.1109/CSGB51356.2020.9214649
M. Surovtseva, O. Poveshchenko, I. Kim, V. Pichugin, I. Zhuravleva
One of the current areas of medical materials science is the formation of ultrathin coatings on the surface of implants. Among the many types of coating used in vascular surgery, binary and ternary titanium compounds have good prospects due to their high bio- and hemocompatibility. The properties of the coating based on films of nitrogen-containing titanium oxide (titanium oxynitride), applied by reactive magnetron sputtering (RMS), was studied. While problems of establishing and maintaining the necessary spraying mode is relevant for the RMS technique. The cytotoxicity of the titanium oxy nitride compounds obtained under various modes of functioning of RMS on endothelial cells line EA.hy 926 was the aim of this report. It was showed that the levels of nitric oxide (NO) production by EA.hy 926 cell line on Day 1 and Day 5 growth under the different samples of nitinol treated with different RMS modes was comparable to control. It was no obtained cytotoxic effect of the nitrogen-containing coatings of titanium oxide obtained by various modes of RMS on EA.hy 926 cell line. While was observed reduced levels of NO production by EA.hy 926 cell line on Day 3.
{"title":"Cytotoxicity of Titanium Oxynitride Obtained by Reactive Magnetron Sputtered on Endothelial Cell Line EA.hy 926","authors":"M. Surovtseva, O. Poveshchenko, I. Kim, V. Pichugin, I. Zhuravleva","doi":"10.1109/CSGB51356.2020.9214649","DOIUrl":"https://doi.org/10.1109/CSGB51356.2020.9214649","url":null,"abstract":"One of the current areas of medical materials science is the formation of ultrathin coatings on the surface of implants. Among the many types of coating used in vascular surgery, binary and ternary titanium compounds have good prospects due to their high bio- and hemocompatibility. The properties of the coating based on films of nitrogen-containing titanium oxide (titanium oxynitride), applied by reactive magnetron sputtering (RMS), was studied. While problems of establishing and maintaining the necessary spraying mode is relevant for the RMS technique. The cytotoxicity of the titanium oxy nitride compounds obtained under various modes of functioning of RMS on endothelial cells line EA.hy 926 was the aim of this report. It was showed that the levels of nitric oxide (NO) production by EA.hy 926 cell line on Day 1 and Day 5 growth under the different samples of nitinol treated with different RMS modes was comparable to control. It was no obtained cytotoxic effect of the nitrogen-containing coatings of titanium oxide obtained by various modes of RMS on EA.hy 926 cell line. While was observed reduced levels of NO production by EA.hy 926 cell line on Day 3.","PeriodicalId":447695,"journal":{"name":"2020 Cognitive Sciences, Genomics and Bioinformatics (CSGB)","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114963802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.1109/CSGB51356.2020.9214681
M. Kulyashov, S. Kolmykov, I. Yevshin, F. Kolpakov
GEO database contains a lot of different experiments about transcription regulation. Most part of such experiments (ChIP-seq, DNase-seq and Histone-ChIP-seq) for 9 species (Homo sapiens, Mus musculus, Rattus norvegicus, Danio rerio, Caenorhabditis elegans, Drosophila melanogaster, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Arabidopsis thaliana) were annotated and uniformly processed in GTRD database. Here we are describing the latest advances in these data annotations for GTRD database to formalize experimental data: hierarchical dictionaries of cell types and experimental factors. This approach helps us to integrate experimental data by cell lines (cell types and tissues) and experimental conditions as well as simplifies searching for relevant information for a user.
{"title":"Advanced data curation in GTRD database: hierarchical dictionaries of cell types and experimental factors","authors":"M. Kulyashov, S. Kolmykov, I. Yevshin, F. Kolpakov","doi":"10.1109/CSGB51356.2020.9214681","DOIUrl":"https://doi.org/10.1109/CSGB51356.2020.9214681","url":null,"abstract":"GEO database contains a lot of different experiments about transcription regulation. Most part of such experiments (ChIP-seq, DNase-seq and Histone-ChIP-seq) for 9 species (Homo sapiens, Mus musculus, Rattus norvegicus, Danio rerio, Caenorhabditis elegans, Drosophila melanogaster, Saccharomyces cerevisiae, Schizosaccharomyces pombe and Arabidopsis thaliana) were annotated and uniformly processed in GTRD database. Here we are describing the latest advances in these data annotations for GTRD database to formalize experimental data: hierarchical dictionaries of cell types and experimental factors. This approach helps us to integrate experimental data by cell lines (cell types and tissues) and experimental conditions as well as simplifies searching for relevant information for a user.","PeriodicalId":447695,"journal":{"name":"2020 Cognitive Sciences, Genomics and Bioinformatics (CSGB)","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121252065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.1109/CSGB51356.2020.9214740
E. Snegireva, Grigory R. Khazankin, I. Mikheenko
Nowadays, analog electrocardiographs that deliver only paper printout are ubiquitous in medical institutions. Doctors do visual analysis of electrocardiograms (ECG), occasionally using measurement tools. This article reviews approaches to automatic analysis of electrocardiogram images, including the signal conversion from paper to digital format. The following methods are presented: digitizing graphs from images, determination of signal nodes, and preparation of final report. Various methods of computer vision were tested on electrocardiogram images in order to highlight the graph and transfer coordinates to millimeters. Their limitations are identified and described. Based on the evaluation, a suitable electrocardiogram analysis method has been developed. It includes color filtering of the background grid. Methods of signal analysis and reading of indicators, and their further analysis, are also given. The text conclusion is based on decision trees traversal. As a result, the architecture of measuring system software for electrocardiogram analysis was developed. The system is described considering that the electrocardiogram evaluation unit does not depend on external implementation and can be reused in other systems performing electrocardiogram analysis.
{"title":"ECG printout interpretation system for clinical decision support","authors":"E. Snegireva, Grigory R. Khazankin, I. Mikheenko","doi":"10.1109/CSGB51356.2020.9214740","DOIUrl":"https://doi.org/10.1109/CSGB51356.2020.9214740","url":null,"abstract":"Nowadays, analog electrocardiographs that deliver only paper printout are ubiquitous in medical institutions. Doctors do visual analysis of electrocardiograms (ECG), occasionally using measurement tools. This article reviews approaches to automatic analysis of electrocardiogram images, including the signal conversion from paper to digital format. The following methods are presented: digitizing graphs from images, determination of signal nodes, and preparation of final report. Various methods of computer vision were tested on electrocardiogram images in order to highlight the graph and transfer coordinates to millimeters. Their limitations are identified and described. Based on the evaluation, a suitable electrocardiogram analysis method has been developed. It includes color filtering of the background grid. Methods of signal analysis and reading of indicators, and their further analysis, are also given. The text conclusion is based on decision trees traversal. As a result, the architecture of measuring system software for electrocardiogram analysis was developed. The system is described considering that the electrocardiogram evaluation unit does not depend on external implementation and can be reused in other systems performing electrocardiogram analysis.","PeriodicalId":447695,"journal":{"name":"2020 Cognitive Sciences, Genomics and Bioinformatics (CSGB)","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124590135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.1109/CSGB51356.2020.9214777
O. Vishnevsky, A. Bocharnikov, N. Kolchanov
Composite elements play an important role in the regulation of transcription. Existing methods for the revealing of potential composite elements are usually based on assessment of the significance of the mutual presence of the predicted transcription factor binding sites using weight matrices or other methods trained on samples of binding sites of known transcription factors. Thus, such methods essentially depend on the completeness of training samples and information on existing TFs. We have proposed a method for de novo discovery of potential composite elements, which does not require preliminary information about the localization of potential TFBS. Using the proposed approach, context signals are identified in the ChIP-Seq dataset, which can correspond to potential composite elements.
{"title":"GPU Based Composite Elements Discovery In Large DNADatasets","authors":"O. Vishnevsky, A. Bocharnikov, N. Kolchanov","doi":"10.1109/CSGB51356.2020.9214777","DOIUrl":"https://doi.org/10.1109/CSGB51356.2020.9214777","url":null,"abstract":"Composite elements play an important role in the regulation of transcription. Existing methods for the revealing of potential composite elements are usually based on assessment of the significance of the mutual presence of the predicted transcription factor binding sites using weight matrices or other methods trained on samples of binding sites of known transcription factors. Thus, such methods essentially depend on the completeness of training samples and information on existing TFs. We have proposed a method for de novo discovery of potential composite elements, which does not require preliminary information about the localization of potential TFBS. Using the proposed approach, context signals are identified in the ChIP-Seq dataset, which can correspond to potential composite elements.","PeriodicalId":447695,"journal":{"name":"2020 Cognitive Sciences, Genomics and Bioinformatics (CSGB)","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129612741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.1109/CSGB51356.2020.9214761
K. Kolpakov, M. Korolev, E. Letyagina, Vitaly Omelchenk, A. Akimova, Julia Kurochkina
Ankylosing spondylitis (AS) is a chronic progressive systemic inflammatory disease, with a primary lesion of the spine and sacroiliac joints characterized by simultaneously running processes of osteogenesis (syndesmophytes) and osteoresorption (osteoporosis). The aim of this work is to investigate trabecular bone score (TBS) correlation with signs of AS structural progression and markers of AS activity in the males before 50. Twenty one AS male patients before 50 without osteoporosis are included in investigation. The relationships between TBS, the Kellgren sacroiliitis stage, and BMD indices in different parts of the skeleton were studied using Spearman correlation analysis. A negative correlation was found between TBS and the X-ray stage of Kellgren sacroiliitis (r = -0.49; p <0.05) in men before 50 with AS. Clinical and laboratory activity did not correlate with TBS. Bone mineral density (BMD) did not reflect the degree of structural disturbances and the risks of spinal fractures in men before 50 with AS in the absence of other causes of secondary osteoporosis.
{"title":"Opportunities of Trabecular Bone Score to Evaluate Ankylosing Spondilitis Structural Progression in Young Male Patients","authors":"K. Kolpakov, M. Korolev, E. Letyagina, Vitaly Omelchenk, A. Akimova, Julia Kurochkina","doi":"10.1109/CSGB51356.2020.9214761","DOIUrl":"https://doi.org/10.1109/CSGB51356.2020.9214761","url":null,"abstract":"Ankylosing spondylitis (AS) is a chronic progressive systemic inflammatory disease, with a primary lesion of the spine and sacroiliac joints characterized by simultaneously running processes of osteogenesis (syndesmophytes) and osteoresorption (osteoporosis). The aim of this work is to investigate trabecular bone score (TBS) correlation with signs of AS structural progression and markers of AS activity in the males before 50. Twenty one AS male patients before 50 without osteoporosis are included in investigation. The relationships between TBS, the Kellgren sacroiliitis stage, and BMD indices in different parts of the skeleton were studied using Spearman correlation analysis. A negative correlation was found between TBS and the X-ray stage of Kellgren sacroiliitis (r = -0.49; p <0.05) in men before 50 with AS. Clinical and laboratory activity did not correlate with TBS. Bone mineral density (BMD) did not reflect the degree of structural disturbances and the risks of spinal fractures in men before 50 with AS in the absence of other causes of secondary osteoporosis.","PeriodicalId":447695,"journal":{"name":"2020 Cognitive Sciences, Genomics and Bioinformatics (CSGB)","volume":"AES-17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126541612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.1109/CSGB51356.2020.9214734
E. Myasnikova, A. Spirov
Evolutionary computations is an impressive example of the convergence of two research fields: evolutionary biology and computer sciences. While initially genetic algorithms (GAs) were inspired by the ideas of the theory of evolution in biology, there is now a tendency toward the reverse transfer of ideas to a biological experiment. An example of such a transfer is the directed evolution of macromolecules that can be considered as an analogue of GAs in biochemical experiments. We focus on the concept of building blocks (BB) that underlies the theoretical and practical effectiveness of GA in evolutionary search. In in vitro experiments the modular structure of macromolecules points to the parallels between domains/motifs and BBs. In computer science there were developed a great number of algorithms for finding and preserving BBs. It was proved that such procedures provide the effectiveness of the evolutionary search. In this work we show how significantly some heuristic algorithms preserving the BBs can increase the efficiency of the in vitro evolution. As a benchmark test, we use such an actual problem of synthetic biology as the evolutionary search for multi-domain RNA devices. The results of these tests with simple heuristic algorithms are very promising with the prospective of further implementation of more advanced GA procedures in the evolutionary experiments in vitro. In conclusion, we discuss the importance of such highly efficient heuristic algorithms for the evolutionary and synthetic biology.
{"title":"Transfer of Genetic Algorithms to Directed Evolution of Macromolecules: Tests in Silico","authors":"E. Myasnikova, A. Spirov","doi":"10.1109/CSGB51356.2020.9214734","DOIUrl":"https://doi.org/10.1109/CSGB51356.2020.9214734","url":null,"abstract":"Evolutionary computations is an impressive example of the convergence of two research fields: evolutionary biology and computer sciences. While initially genetic algorithms (GAs) were inspired by the ideas of the theory of evolution in biology, there is now a tendency toward the reverse transfer of ideas to a biological experiment. An example of such a transfer is the directed evolution of macromolecules that can be considered as an analogue of GAs in biochemical experiments. We focus on the concept of building blocks (BB) that underlies the theoretical and practical effectiveness of GA in evolutionary search. In in vitro experiments the modular structure of macromolecules points to the parallels between domains/motifs and BBs. In computer science there were developed a great number of algorithms for finding and preserving BBs. It was proved that such procedures provide the effectiveness of the evolutionary search. In this work we show how significantly some heuristic algorithms preserving the BBs can increase the efficiency of the in vitro evolution. As a benchmark test, we use such an actual problem of synthetic biology as the evolutionary search for multi-domain RNA devices. The results of these tests with simple heuristic algorithms are very promising with the prospective of further implementation of more advanced GA procedures in the evolutionary experiments in vitro. In conclusion, we discuss the importance of such highly efficient heuristic algorithms for the evolutionary and synthetic biology.","PeriodicalId":447695,"journal":{"name":"2020 Cognitive Sciences, Genomics and Bioinformatics (CSGB)","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130958094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.1109/CSGB51356.2020.9214720
M. Vladimirova, A. Afonin, V. Muntyan, B. Simarov, M. Roumiantseva
Transfer RNAs (tRNA) widely abundant in genomes are extremely conservative molecules with well-defined role in protein synthesis. The central role of tRNA in the evolution of the genetic code and theirs novel undiscovered functions were recently predicted: these sequences could be a hotspots for the integration of foreign genomic DNA, like phage related genomic islands (GIs). In this work the 17th GIs site-specifically integrated into isoacceptors tRNAs-Thr (isotRNA-Thr) out of 84th GIs detected in genomes of Sinorhizobium meliloti strains were evaluated. It was shown that the homology of entire sequences of GIs inserted into a particular tRNA isoacceptor in genetically related strains is more than 80%, whereas homology of similar types of GIs in native strains from one population or from geographically remote areas could be distinct up to 70%. For the first time CRISPRs sequences were revealed in some GIs of S. meliloti. The phylogenetic analysis done for integrase encoding sequences of distinct GIs inserted in iso-tRNAs-Thr showed their homology with the corresponding sequences of phages from Podovirida, Myoviridae and Siphoviridae families. Proceeded data allowed concluding that the insertion of GIs into precise tRNA-Tre isoaceptor occurred due to the att-homologous recombination mechanism and that GIs of S. meliloti could be involved in improving adaptive immunity of rhizobia.
{"title":"Evaluation of Sinorhizobium meliloti Genomic Islands Inserted into the tRNA-Thr","authors":"M. Vladimirova, A. Afonin, V. Muntyan, B. Simarov, M. Roumiantseva","doi":"10.1109/CSGB51356.2020.9214720","DOIUrl":"https://doi.org/10.1109/CSGB51356.2020.9214720","url":null,"abstract":"Transfer RNAs (tRNA) widely abundant in genomes are extremely conservative molecules with well-defined role in protein synthesis. The central role of tRNA in the evolution of the genetic code and theirs novel undiscovered functions were recently predicted: these sequences could be a hotspots for the integration of foreign genomic DNA, like phage related genomic islands (GIs). In this work the 17th GIs site-specifically integrated into isoacceptors tRNAs-Thr (isotRNA-Thr) out of 84th GIs detected in genomes of Sinorhizobium meliloti strains were evaluated. It was shown that the homology of entire sequences of GIs inserted into a particular tRNA isoacceptor in genetically related strains is more than 80%, whereas homology of similar types of GIs in native strains from one population or from geographically remote areas could be distinct up to 70%. For the first time CRISPRs sequences were revealed in some GIs of S. meliloti. The phylogenetic analysis done for integrase encoding sequences of distinct GIs inserted in iso-tRNAs-Thr showed their homology with the corresponding sequences of phages from Podovirida, Myoviridae and Siphoviridae families. Proceeded data allowed concluding that the insertion of GIs into precise tRNA-Tre isoaceptor occurred due to the att-homologous recombination mechanism and that GIs of S. meliloti could be involved in improving adaptive immunity of rhizobia.","PeriodicalId":447695,"journal":{"name":"2020 Cognitive Sciences, Genomics and Bioinformatics (CSGB)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131875990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.1109/CSGB51356.2020.9214705
Arthur I. Dergilev, Y. Orlov
This work presents the results of using computer scripts to analyze ChIP-seq data, calculate clusters, and visualize them in the form of heat maps. In the work, ChIP-seq peaks were used to study the stem cell niche of three plants, among which the well-known Talus (Arabidopsis Thailana), Physcomitrella patens, Chlamydomonas reinhardti. What about the tendency of co-localization of transcription factors in plant genomes? What are the features of this co-localization?
{"title":"Statistical problems of clusters of transcription factor binding sites in plant genomes","authors":"Arthur I. Dergilev, Y. Orlov","doi":"10.1109/CSGB51356.2020.9214705","DOIUrl":"https://doi.org/10.1109/CSGB51356.2020.9214705","url":null,"abstract":"This work presents the results of using computer scripts to analyze ChIP-seq data, calculate clusters, and visualize them in the form of heat maps. In the work, ChIP-seq peaks were used to study the stem cell niche of three plants, among which the well-known Talus (Arabidopsis Thailana), Physcomitrella patens, Chlamydomonas reinhardti. What about the tendency of co-localization of transcription factors in plant genomes? What are the features of this co-localization?","PeriodicalId":447695,"journal":{"name":"2020 Cognitive Sciences, Genomics and Bioinformatics (CSGB)","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123963715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.1109/CSGB51356.2020.9214658
J. Semenova, M. V. Dashkin
Background and aim:Continuous glucose monitoring (CGM) provides an excellent opportunity for in-depth assessment of glycemic control and glucose variability (GV) in diabetic subjects.The aim of our study was to determine the clinical and metabolic parameters associated with non-targeted time in range (TIR) increased GV in patients with type 2 diabetes (T2D) treated with insulin.Materials and Methods: One hundred and thirty six insulin-treated patients with T2Dwere included. Real-time or blinded CGM was performed using Medtronic CGM devices. The TIR and Mean Amplitude of Glucose Excursion (MAGE) were estimated. The advance glycation end-products (AGEs) levels were measured in blood serum by ELISA.Results: Patients with non-targeted TIR (>70%) hadhigher glycated hemoglobin HbA1c, triglycerides and proteinuria as compared to those with targeted TIR. Urinary albumin-to-creatinine ratio tended to be higher in patients with non-targeted TIR also. Patients with higher MAGE (>4.5 mmol/l) demonstrated lower levels of triglycerides and uric acid andincreased AGEs levels as compared to those with MAGE <4.5 mmol/l.Conclusions:In T2D subjects, non-targeted TIR is associated with hypertriglyceridemia and proteinuria, meantime, increased MAGE is related to lower serum levels of triglycerides and uric acid and higher levels of AGEs.
{"title":"Clinical and metabolic parameters associated with time in ranges and glucose variability in patients with type 2 diabetes treated with insulin","authors":"J. Semenova, M. V. Dashkin","doi":"10.1109/CSGB51356.2020.9214658","DOIUrl":"https://doi.org/10.1109/CSGB51356.2020.9214658","url":null,"abstract":"Background and aim:Continuous glucose monitoring (CGM) provides an excellent opportunity for in-depth assessment of glycemic control and glucose variability (GV) in diabetic subjects.The aim of our study was to determine the clinical and metabolic parameters associated with non-targeted time in range (TIR) increased GV in patients with type 2 diabetes (T2D) treated with insulin.Materials and Methods: One hundred and thirty six insulin-treated patients with T2Dwere included. Real-time or blinded CGM was performed using Medtronic CGM devices. The TIR and Mean Amplitude of Glucose Excursion (MAGE) were estimated. The advance glycation end-products (AGEs) levels were measured in blood serum by ELISA.Results: Patients with non-targeted TIR (>70%) hadhigher glycated hemoglobin HbA1c, triglycerides and proteinuria as compared to those with targeted TIR. Urinary albumin-to-creatinine ratio tended to be higher in patients with non-targeted TIR also. Patients with higher MAGE (>4.5 mmol/l) demonstrated lower levels of triglycerides and uric acid andincreased AGEs levels as compared to those with MAGE <4.5 mmol/l.Conclusions:In T2D subjects, non-targeted TIR is associated with hypertriglyceridemia and proteinuria, meantime, increased MAGE is related to lower serum levels of triglycerides and uric acid and higher levels of AGEs.","PeriodicalId":447695,"journal":{"name":"2020 Cognitive Sciences, Genomics and Bioinformatics (CSGB)","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125902862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.1109/CSGB51356.2020.9214773
A. Lykov, E. Rachkovsky, Ruslan Gevorgiz, S. Zheleznova, A. Kotlyarova
This work described toxicity of composition of fucoxanthin with particles of porous aluminum oxide with polydimethylsiloxane (Fx@Al/PDMS) in mice lymphocytes. Effects of Fx@Al/PDMS on Balb/c mice splenocytes and thymocytes viability, and proliferation, and intracellular myeloperoxidase activity, and nitric oxide (NO) production, and apoptosis were studied. It was shown that short-term (24 h) and long-term (129 h) incubation lymphocytes with Fx@Al/PDMS did not have any adverse effect on functional properties of splenocytes and thymocytes.
{"title":"Toxicity of Fucoxanthin on Balb/c Mice Splenocytes and Thymocytes","authors":"A. Lykov, E. Rachkovsky, Ruslan Gevorgiz, S. Zheleznova, A. Kotlyarova","doi":"10.1109/CSGB51356.2020.9214773","DOIUrl":"https://doi.org/10.1109/CSGB51356.2020.9214773","url":null,"abstract":"This work described toxicity of composition of fucoxanthin with particles of porous aluminum oxide with polydimethylsiloxane (Fx@Al/PDMS) in mice lymphocytes. Effects of Fx@Al/PDMS on Balb/c mice splenocytes and thymocytes viability, and proliferation, and intracellular myeloperoxidase activity, and nitric oxide (NO) production, and apoptosis were studied. It was shown that short-term (24 h) and long-term (129 h) incubation lymphocytes with Fx@Al/PDMS did not have any adverse effect on functional properties of splenocytes and thymocytes.","PeriodicalId":447695,"journal":{"name":"2020 Cognitive Sciences, Genomics and Bioinformatics (CSGB)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128305720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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