Pub Date : 2020-01-01DOI: 10.21608/ejaps.2020.175316
Heba F. A. Elkomey, M. Mohamed, Sohier S. Korraa, M. Hegazy
. Radiation-induced bystander effect (RIBE), is well verified in radiation biology field. Vast bulk of these effects are defined in cell culture systems, while in vivo authentication and evaluation of biological consequences still vague. We aimed to mechanistically interrogate RIBE responses thus this study was conducted. Accordingly, Albino rats’ right femurs were exposed to 0.25, 0.45 and 0.75 Gу γ-radiation. Rats were euthanized 1 day and ten days post irradiation, total antioxidant capacity and mRNA expression levels of Stromal Derived Growth Factor (SDF-1) were evaluated in bone marrow. Blood micronucleated polychromatic erythrocytes (fMNPCEs) and femoral muscle’s lipid peroxidation (LPO) were measurable. Femoral muscle’s LPO, total antioxidant capacity and mRNA genes expression levels were significantly increased in both left and right femur at 0.45 and 0.75 Gy 24 h post irradiation and retained near control levels 10 days post irradiation. fMNPCEs became significantly higher 24 h post irradiation and showed a non-significant difference at all doses ten days post irradiation. In conclusion, the increase of LPO in femur muscle, with elevation in SDF-1 mRNA expression levels in bone marrow, and total antioxidant capacity in non-irradiated left femurs indicate that a bystander effect exists.
{"title":"Assessment of the bystander effect of low dose gamma radiation on rats’ bone marrow","authors":"Heba F. A. Elkomey, M. Mohamed, Sohier S. Korraa, M. Hegazy","doi":"10.21608/ejaps.2020.175316","DOIUrl":"https://doi.org/10.21608/ejaps.2020.175316","url":null,"abstract":". Radiation-induced bystander effect (RIBE), is well verified in radiation biology field. Vast bulk of these effects are defined in cell culture systems, while in vivo authentication and evaluation of biological consequences still vague. We aimed to mechanistically interrogate RIBE responses thus this study was conducted. Accordingly, Albino rats’ right femurs were exposed to 0.25, 0.45 and 0.75 Gу γ-radiation. Rats were euthanized 1 day and ten days post irradiation, total antioxidant capacity and mRNA expression levels of Stromal Derived Growth Factor (SDF-1) were evaluated in bone marrow. Blood micronucleated polychromatic erythrocytes (fMNPCEs) and femoral muscle’s lipid peroxidation (LPO) were measurable. Femoral muscle’s LPO, total antioxidant capacity and mRNA genes expression levels were significantly increased in both left and right femur at 0.45 and 0.75 Gy 24 h post irradiation and retained near control levels 10 days post irradiation. fMNPCEs became significantly higher 24 h post irradiation and showed a non-significant difference at all doses ten days post irradiation. In conclusion, the increase of LPO in femur muscle, with elevation in SDF-1 mRNA expression levels in bone marrow, and total antioxidant capacity in non-irradiated left femurs indicate that a bystander effect exists.","PeriodicalId":447760,"journal":{"name":"Egyptian Journal of Applied and Pure Science","volume":"295 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117066331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.21608/ejaps.2021.175319
{"title":"Groundwater flow model of the quaternary aquifer in El Khatatba area for water resources management","authors":"","doi":"10.21608/ejaps.2021.175319","DOIUrl":"https://doi.org/10.21608/ejaps.2021.175319","url":null,"abstract":"","PeriodicalId":447760,"journal":{"name":"Egyptian Journal of Applied and Pure Science","volume":"28 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133111394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.21608/ejaps.2020.175315
M. Farghali, M. Abo-aly, T. Salaheldin
This study discusses the capability of the prepared modified mesoporous zeolite-A /reduced graphene oxide nanocomposite (MZ-A/RGO) in removal of cadmium (Cd 2+ ) ions from wastewater, in which 3-aminopropyl- trimethoxysilane (APTMS) was used as a mesopore generating and cross linkage agent in this preparation. X-ray diffraction, attenuated total reflectance- Fourier transform infrared spectroscopy (ATR-FTIR) and field emission scanning electron microscope were used to confirm the successful preparation of MZ-A/RGO nanocomposite. The results indicated that the modified mesoporous zeolite-A (MZ-A) was attached to the surface of the reduced graphene oxide (RGO) nanosheets without any aggregation. The adsorption treatment experiments were conducted for Cd 2+ ions removal and the adsorption capacities of MZ-A/RGO were determined under different adsorption parameters including, the contact time, initial adsorbate concentrations and temperature. The adsorption results revealed that 15 minutes was quiet enough to ensure the equilibrium state with maximum adsorption capacity 222.23 mg/g and the experimental data was fitted well with the pseudo-second-order kinetic model. The isotherm studies refer to the Langmuir model exhibits best fitting for the obtained experimental data and the thermodynamic results confirm that the endothermic nature of the adsorption of Cd 2+ ions on MZ-A/RGO nanocomposite.
{"title":"Effective removal of cadmium ions from wastewater using modified mesoporous zeolite-A /reduced graphene oxide nanocomposite: kinetic and thermodynamic studies","authors":"M. Farghali, M. Abo-aly, T. Salaheldin","doi":"10.21608/ejaps.2020.175315","DOIUrl":"https://doi.org/10.21608/ejaps.2020.175315","url":null,"abstract":"This study discusses the capability of the prepared modified mesoporous zeolite-A /reduced graphene oxide nanocomposite (MZ-A/RGO) in removal of cadmium (Cd 2+ ) ions from wastewater, in which 3-aminopropyl- trimethoxysilane (APTMS) was used as a mesopore generating and cross linkage agent in this preparation. X-ray diffraction, attenuated total reflectance- Fourier transform infrared spectroscopy (ATR-FTIR) and field emission scanning electron microscope were used to confirm the successful preparation of MZ-A/RGO nanocomposite. The results indicated that the modified mesoporous zeolite-A (MZ-A) was attached to the surface of the reduced graphene oxide (RGO) nanosheets without any aggregation. The adsorption treatment experiments were conducted for Cd 2+ ions removal and the adsorption capacities of MZ-A/RGO were determined under different adsorption parameters including, the contact time, initial adsorbate concentrations and temperature. The adsorption results revealed that 15 minutes was quiet enough to ensure the equilibrium state with maximum adsorption capacity 222.23 mg/g and the experimental data was fitted well with the pseudo-second-order kinetic model. The isotherm studies refer to the Langmuir model exhibits best fitting for the obtained experimental data and the thermodynamic results confirm that the endothermic nature of the adsorption of Cd 2+ ions on MZ-A/RGO nanocomposite.","PeriodicalId":447760,"journal":{"name":"Egyptian Journal of Applied and Pure Science","volume":"100 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127320041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.21608/ejaps.2020.175313
{"title":"Lack of association between vitamin D receptor Taq I polymorphism and diabetic nephropathy in type 2 diabetes mellitus patients","authors":"","doi":"10.21608/ejaps.2020.175313","DOIUrl":"https://doi.org/10.21608/ejaps.2020.175313","url":null,"abstract":"","PeriodicalId":447760,"journal":{"name":"Egyptian Journal of Applied and Pure Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125879935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.21608/ejaps.2020.175314
Fayrouz A. Muhammad, Mahmoud N. El-Rouby, M. M. Said, A. Esmat
Article history: Received 19 July 2020 Accepted 19 August 2020 Epithelial-mesenchymal transition (EMT) is a biologic process that permits an epithelial cell to undergo multiple biochemical changes that enable it to assume a mesenchymal cell phenotype with enhanced migratory capacity and invasiveness. In the present study, the cytotoxic activity of aloin, a natural anthraquinone glycoside, was assessed against a breast cancer cell line (T47D) using MTT and clonogenic assays, compared to doxorubicin, an anthracycline analog. The effects of exposure of T47D tumor cells to IC50 values of aloin and doxorubicin (181.5 and 0.17 μM, respectively) for 72 h on the mRNA expression levels of a nonreceptor tyrosine kinase (JAK2), a transcription factor (STAT5a) and some biomarkers for EMT were evaluated to provide new prognostic and therapeutic markers for estrogen receptor-positive breast cancer progression. Exposure to aloin down-regulated the expression levels of JAK2, STAT5a and vimentin mRNA in T47D cells, while the expression levels of E-cadherin and ZO-1 mRNA were not significantly changed. On the other hand, exposure of breast tumor cells to doxorubicin up-regulated the expression of E-cadherin mRNA, whereas the expression levels of JAK2, STAT5a, ZO-1 and vimentin mRNA were not affected. In conclusion, the chemosensitivity of aloin towards breast tumor cells is due to inhibition of JAK2/STAT5a signaling, which results in the inhibition of vimentin expression as a mesenchymal marker of EMT.
{"title":"Effect of aloin on some gene biomarkers for epithelial-mesenchymal transition in breast cancer in vitro compared to an anthracycline analog (Doxorubicin)","authors":"Fayrouz A. Muhammad, Mahmoud N. El-Rouby, M. M. Said, A. Esmat","doi":"10.21608/ejaps.2020.175314","DOIUrl":"https://doi.org/10.21608/ejaps.2020.175314","url":null,"abstract":"Article history: Received 19 July 2020 Accepted 19 August 2020 Epithelial-mesenchymal transition (EMT) is a biologic process that permits an epithelial cell to undergo multiple biochemical changes that enable it to assume a mesenchymal cell phenotype with enhanced migratory capacity and invasiveness. In the present study, the cytotoxic activity of aloin, a natural anthraquinone glycoside, was assessed against a breast cancer cell line (T47D) using MTT and clonogenic assays, compared to doxorubicin, an anthracycline analog. The effects of exposure of T47D tumor cells to IC50 values of aloin and doxorubicin (181.5 and 0.17 μM, respectively) for 72 h on the mRNA expression levels of a nonreceptor tyrosine kinase (JAK2), a transcription factor (STAT5a) and some biomarkers for EMT were evaluated to provide new prognostic and therapeutic markers for estrogen receptor-positive breast cancer progression. Exposure to aloin down-regulated the expression levels of JAK2, STAT5a and vimentin mRNA in T47D cells, while the expression levels of E-cadherin and ZO-1 mRNA were not significantly changed. On the other hand, exposure of breast tumor cells to doxorubicin up-regulated the expression of E-cadherin mRNA, whereas the expression levels of JAK2, STAT5a, ZO-1 and vimentin mRNA were not affected. In conclusion, the chemosensitivity of aloin towards breast tumor cells is due to inhibition of JAK2/STAT5a signaling, which results in the inhibition of vimentin expression as a mesenchymal marker of EMT.","PeriodicalId":447760,"journal":{"name":"Egyptian Journal of Applied and Pure Science","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127298606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.21608/ejaps.2021.175318
{"title":"Synthesis and characterization of Nano Zirconia for use in Chromatographic 188W/188Re","authors":"","doi":"10.21608/ejaps.2021.175318","DOIUrl":"https://doi.org/10.21608/ejaps.2021.175318","url":null,"abstract":"","PeriodicalId":447760,"journal":{"name":"Egyptian Journal of Applied and Pure Science","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125719255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.21608/ejaps.2019.175306
Aya M. Mohamed, Nashwa El-Khazragy, M. M. Said, M. Swellam, A. Esmat
Article history: Received 15 September 2019 Accepted 21 October 2019 The present study was undertaken to investigate the relative expression levels of miRNA-100 and -196b in childhood acute lymphoblastic leukemia (ALL) and its phenotypes. Peripheral blood mononuclear cells (PMNCs) were isolated from peripheral blood samples of 40 pediatric ALL patients and 10 healthy controls. We assessed the expression levels of miRNA-100 and -196b by quantitative real time polymerase chain reaction (qRT-PCR) assay. A significant upregulation in the expression levels of miRNA-100 and -196b in ALL patients is reported, compared to the normal controls. T-ALL patients manifested a higher expression of miRNA-100 and-196b than those with pre-B-ALL and biphenotypic ALL. MiRNA-100 and -196b distinguished ALL patients from the normal controls at cut-off values 6.54 (92.5 % sensitivity, 100 % specificity) and 5.49 (92.5 % sensitivity, 100 % specificity), respectively. As well, miRNA-100 and -196b discriminated between the different ALL phenotypes with high sensitivity, specificity and accuracy levels. Two correlation coefficients are herein reported including a significant positive correlation between miRNA-100 and -196b expression levels (r = 0.328, p < 0.05) and a significant negative correlation between miRNA-100 expression level and the platelets count (r = -0.448, p < 0.01) in ALL patients. Our findings concluded that miRNA-100 and -196b could be considered as good noninvasive blood biomarkers for the diagnosis of ALL, and in distinguishing its phenotypes.
本研究旨在探讨miRNA-100和-196b在儿童急性淋巴细胞白血病(ALL)及其表型中的相对表达水平。从40例小儿ALL患者和10例健康对照者的外周血中分离外周血单个核细胞(pmnc)。采用定量实时聚合酶链反应(qRT-PCR)法检测miRNA-100和-196b的表达水平。据报道，与正常对照组相比，ALL患者中miRNA-100和-196b的表达水平显著上调。T-ALL患者miRNA-100和196b的表达高于b -ALL前期和双表型ALL患者。MiRNA-100和-196b区分ALL患者和正常对照的临界值分别为6.54(92.5%敏感性，100%特异性)和5.49(92.5%敏感性，100%特异性)。此外，miRNA-100和-196b在ALL不同表型之间具有较高的敏感性、特异性和准确性。在ALL患者中，miRNA-100与-196b表达水平呈显著正相关(r = 0.328, p < 0.05)， miRNA-100表达水平与血小板计数呈显著负相关(r = -0.448, p < 0.01)。我们的研究结果表明，miRNA-100和-196b可以被认为是诊断ALL和区分其表型的良好的无创血液生物标志物。
{"title":"Diagnostic significance of microRNA-100 and -196b as blood markers for acute lymphoblastic leukemia among children","authors":"Aya M. Mohamed, Nashwa El-Khazragy, M. M. Said, M. Swellam, A. Esmat","doi":"10.21608/ejaps.2019.175306","DOIUrl":"https://doi.org/10.21608/ejaps.2019.175306","url":null,"abstract":"Article history: Received 15 September 2019 Accepted 21 October 2019 The present study was undertaken to investigate the relative expression levels of miRNA-100 and -196b in childhood acute lymphoblastic leukemia (ALL) and its phenotypes. Peripheral blood mononuclear cells (PMNCs) were isolated from peripheral blood samples of 40 pediatric ALL patients and 10 healthy controls. We assessed the expression levels of miRNA-100 and -196b by quantitative real time polymerase chain reaction (qRT-PCR) assay. A significant upregulation in the expression levels of miRNA-100 and -196b in ALL patients is reported, compared to the normal controls. T-ALL patients manifested a higher expression of miRNA-100 and-196b than those with pre-B-ALL and biphenotypic ALL. MiRNA-100 and -196b distinguished ALL patients from the normal controls at cut-off values 6.54 (92.5 % sensitivity, 100 % specificity) and 5.49 (92.5 % sensitivity, 100 % specificity), respectively. As well, miRNA-100 and -196b discriminated between the different ALL phenotypes with high sensitivity, specificity and accuracy levels. Two correlation coefficients are herein reported including a significant positive correlation between miRNA-100 and -196b expression levels (r = 0.328, p < 0.05) and a significant negative correlation between miRNA-100 expression level and the platelets count (r = -0.448, p < 0.01) in ALL patients. Our findings concluded that miRNA-100 and -196b could be considered as good noninvasive blood biomarkers for the diagnosis of ALL, and in distinguishing its phenotypes.","PeriodicalId":447760,"journal":{"name":"Egyptian Journal of Applied and Pure Science","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126401020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.21608/ejaps.2019.175303
M. H. Bakr, M. Ibrahim, E. H. El-Mosallamy, M. Mekewi
Article history: Received 18 August 2019 Accepted 30 September 2019 A new series of hyperbranched polyester-type polymers were synthesized from a variety of comonomers like adipic acid, 1,2-cyclohexanedicarboxylic anhydride, phthalic anhydride, trimethylolpropane, glycerol, trimellitic anhydride and citric acid using dibutyltin oxide as catalyst according to two steps one pot A2+B3 approach by solution polymerization with a fixed monomers ratio. The consequential polymers were brittle, soluble in ethanol, ethyl acetate and methylethylketone. The prepared polymers were utilized as a novel film forming co-binder with nitrocellulose for solvent based printing inks application. The physico-chemical characteristics were confirmed by FT-IR, GPC and acid value as well as hydroxyl value determinations. The mechanical and optical properties of the produced inks printed film were evaluated. The results show that the overall properties of the inks are of promise quality for use as co-binder resins for solvent based printing inks with 50 % partial substitution in formulation.
{"title":"Value-added ground work of polyester film forming co-binder for flexographic solvent based printing inks","authors":"M. H. Bakr, M. Ibrahim, E. H. El-Mosallamy, M. Mekewi","doi":"10.21608/ejaps.2019.175303","DOIUrl":"https://doi.org/10.21608/ejaps.2019.175303","url":null,"abstract":"Article history: Received 18 August 2019 Accepted 30 September 2019 A new series of hyperbranched polyester-type polymers were synthesized from a variety of comonomers like adipic acid, 1,2-cyclohexanedicarboxylic anhydride, phthalic anhydride, trimethylolpropane, glycerol, trimellitic anhydride and citric acid using dibutyltin oxide as catalyst according to two steps one pot A2+B3 approach by solution polymerization with a fixed monomers ratio. The consequential polymers were brittle, soluble in ethanol, ethyl acetate and methylethylketone. The prepared polymers were utilized as a novel film forming co-binder with nitrocellulose for solvent based printing inks application. The physico-chemical characteristics were confirmed by FT-IR, GPC and acid value as well as hydroxyl value determinations. The mechanical and optical properties of the produced inks printed film were evaluated. The results show that the overall properties of the inks are of promise quality for use as co-binder resins for solvent based printing inks with 50 % partial substitution in formulation.","PeriodicalId":447760,"journal":{"name":"Egyptian Journal of Applied and Pure Science","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115715665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.21608/ejaps.2020.175309
N. Magdy, A. A. Hashim, I. Emara, F. Hamid, N. Abdallah
Diabetes is well-known disease for its complication and increasing population over time, therefore new methods for complication assessment should be found. The aim of this study is to determine levels of nesfatin-1, von Willebrand factor (vWF) and N-acetyl-β-D-glucosaminidase (NAG) in Egyptian type 2 diabetes (T2DM) with and without complications, their assessment as possible markers in tracing diabetes progression in addition to finding a correlation between these three parameters. Sixty-seven Egyptian male patients were divided into three groups as follows: 25 with T2DM without any complication, 22 with diabetic nephropathy (DN) and 20 patients with diabetic cardiovascular complications (DC). Nesfatin-1, vWF, NAG, HOMA-IR (Homeostasis model assessment of insulin resistance), HOMA-β (Homeostasis model assessment of β-cell function) were all determined using ELISA technique , while the fasting blood glucose (FBG), lipid profile and the kidney parameters were analyzed using the biochemistry system, results were compared to 19 healthy controls with same age. Correlation studies were done between all the studied parameters. All diabetic patients showed a significant increase compared to control in the following parameters: (FBG), glycosylated haemoglobin (HbA 1C ), HOMA-IR, serum urea, serum creatinine, albumin creatinine ratio (ACR), triglyceride (TG), total cholesterol (TC), atherogenic indices. Significant decrease was seen in HOMA-β, meanwhile LDL-C and HDL-C showed no statistical significance change compared to control. The studied parameters nesfatin-1 and vWF showed statistical significance increase with all diabetic groups, while NAG showed significance increase only with (DN). In conclusions, there was direct correlations between (nesfatin-1 and HOMA-β), (vWF and ACR) and (HbA 1C and FBG). No correlation was found between the three studied parameters, however these three markers showed significant increase relative to control where nesfatin-1, vWF can be used in tracing of both complications (DN and DC), and NAG can be used only in tracing of DN.
糖尿病是一种众所周知的并发症，而且随着时间的推移，患者数量也在不断增加，因此需要寻找新的并发症评估方法。本研究的目的是确定nesfatin-1、血管性血友病因子(vWF)和n-乙酰-β- d -氨基葡萄糖苷酶(NAG)在埃及2型糖尿病(T2DM)中有无并发症的水平，除了发现这三个参数之间的相关性外，它们的评估还可以作为追踪糖尿病进展的可能标志。67例埃及男性患者分为3组:无并发症T2DM 25例，糖尿病肾病(DN) 22例，糖尿病心血管并发症(DC) 20例。采用ELISA技术检测Nesfatin-1、vWF、NAG、HOMA- ir(胰岛素抵抗的稳态模型评估)、HOMA-β (β细胞功能的稳态模型评估)，采用生化系统分析空腹血糖(FBG)、血脂和肾脏参数，并与19例同龄健康对照进行比较。对所有研究参数进行相关性研究。与对照组相比，所有糖尿病患者的下列指标均有显著升高:空腹血糖(FBG)、糖化血红蛋白(HbA 1C)、HOMA-IR、血清尿素、血清肌酐、白蛋白肌酐比(ACR)、甘油三酯(TG)、总胆固醇(TC)、动脉粥样硬化指标。HOMA-β明显降低，LDL-C、HDL-C与对照组比较无统计学意义。研究参数nesfatin-1和vWF在所有糖尿病组中均有统计学意义升高，而NAG仅在DN组中有统计学意义升高。综上所述，nesfatin-1和HOMA-β、vWF和ACR、HbA 1C和FBG之间存在直接相关性。这三个指标之间没有相关性，但这三个指标相对于对照组有显著增加，其中nesfatin-1、vWF可用于DN和DC两种并发症的追踪，而NAG仅可用于DN。
{"title":"Evaluation of selected diagnostic biomarkers with cardiovascular and nephrotic complications in diabetic Egyptian males","authors":"N. Magdy, A. A. Hashim, I. Emara, F. Hamid, N. Abdallah","doi":"10.21608/ejaps.2020.175309","DOIUrl":"https://doi.org/10.21608/ejaps.2020.175309","url":null,"abstract":"Diabetes is well-known disease for its complication and increasing population over time, therefore new methods for complication assessment should be found. The aim of this study is to determine levels of nesfatin-1, von Willebrand factor (vWF) and N-acetyl-β-D-glucosaminidase (NAG) in Egyptian type 2 diabetes (T2DM) with and without complications, their assessment as possible markers in tracing diabetes progression in addition to finding a correlation between these three parameters. Sixty-seven Egyptian male patients were divided into three groups as follows: 25 with T2DM without any complication, 22 with diabetic nephropathy (DN) and 20 patients with diabetic cardiovascular complications (DC). Nesfatin-1, vWF, NAG, HOMA-IR (Homeostasis model assessment of insulin resistance), HOMA-β (Homeostasis model assessment of β-cell function) were all determined using ELISA technique , while the fasting blood glucose (FBG), lipid profile and the kidney parameters were analyzed using the biochemistry system, results were compared to 19 healthy controls with same age. Correlation studies were done between all the studied parameters. All diabetic patients showed a significant increase compared to control in the following parameters: (FBG), glycosylated haemoglobin (HbA 1C ), HOMA-IR, serum urea, serum creatinine, albumin creatinine ratio (ACR), triglyceride (TG), total cholesterol (TC), atherogenic indices. Significant decrease was seen in HOMA-β, meanwhile LDL-C and HDL-C showed no statistical significance change compared to control. The studied parameters nesfatin-1 and vWF showed statistical significance increase with all diabetic groups, while NAG showed significance increase only with (DN). In conclusions, there was direct correlations between (nesfatin-1 and HOMA-β), (vWF and ACR) and (HbA 1C and FBG). No correlation was found between the three studied parameters, however these three markers showed significant increase relative to control where nesfatin-1, vWF can be used in tracing of both complications (DN and DC), and NAG can be used only in tracing of DN.","PeriodicalId":447760,"journal":{"name":"Egyptian Journal of Applied and Pure Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123803716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.21608/ejaps.2019.175304
W. S. Hafez, S. Mohamed, T. A. Lasheen, D. Sabry, M. Gado
Article history: Received 20 August 2019 Accepted 15 October 2019 The phase-separation phenomenon of non-ionic surfactants occurring in aqueous solution was used for the extraction and concentration of thorium ions. The method is based on the complexation reaction of thorium(IV) with 2-(5-bromo-2pyridylazo)-5-diethylaminophenol (5-Br-PADAP) at pH (3.4). The formed complex was quantitatively extracted to the phase rich in octylphenoxypolyethoxyethanol (Triton X-114) after centrifugation. The enriched analyte in the surfactant-rich phase was extracted, dissolved and determined spectrophotometrically at 531 nm. The optimal extraction and reaction conditions (e.g. pH, surfactant concentration, reagent concentration, temperature, effect of time) were evaluated and the analytical characteristics of the method (e.g. limit of detection, linear range and preconcentration factor) were obtained. Linearity was obeyed in the range of 0.125-2.5μg/ml of Th(IV). The interference effect of some foreign ions was also tested. The proposed method was successfully applied for the preconcentration and determination of trace thorium ions in certified reference sample and silicate geological samples.
{"title":"Cloud Point Extraction and Spectrophotometric Determination of Thorium(IV) Ions and their Application in Silicate Rock Samples","authors":"W. S. Hafez, S. Mohamed, T. A. Lasheen, D. Sabry, M. Gado","doi":"10.21608/ejaps.2019.175304","DOIUrl":"https://doi.org/10.21608/ejaps.2019.175304","url":null,"abstract":"Article history: Received 20 August 2019 Accepted 15 October 2019 The phase-separation phenomenon of non-ionic surfactants occurring in aqueous solution was used for the extraction and concentration of thorium ions. The method is based on the complexation reaction of thorium(IV) with 2-(5-bromo-2pyridylazo)-5-diethylaminophenol (5-Br-PADAP) at pH (3.4). The formed complex was quantitatively extracted to the phase rich in octylphenoxypolyethoxyethanol (Triton X-114) after centrifugation. The enriched analyte in the surfactant-rich phase was extracted, dissolved and determined spectrophotometrically at 531 nm. The optimal extraction and reaction conditions (e.g. pH, surfactant concentration, reagent concentration, temperature, effect of time) were evaluated and the analytical characteristics of the method (e.g. limit of detection, linear range and preconcentration factor) were obtained. Linearity was obeyed in the range of 0.125-2.5μg/ml of Th(IV). The interference effect of some foreign ions was also tested. The proposed method was successfully applied for the preconcentration and determination of trace thorium ions in certified reference sample and silicate geological samples.","PeriodicalId":447760,"journal":{"name":"Egyptian Journal of Applied and Pure Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130079054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：