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Evaluation of Pleurotus Species (P. ostreatus and P. eryngii) Fruiting Potential within the Dry and Rainy Seasons 枯水期和雨季平菇(P. ostreatus和P. eryngii)结实潜力的评价
Pub Date : 2023-10-14 DOI: 10.56201/jbgr.v9.no2.2023.pg1.8
S. S. Agbagwa, E. C. Chuku, A. B. Nwauzoma, F. W. Nmom
Studies on two species of Pleurotus (P. ostreatus and P. eryngii) fruiting potentials within the dry and rainy seasons were carried out in the Department of Plant Science and Biotechnology, Rivers State University. Both species (P. ostreatus; P1 and P. eryngii; P2) were grown on hard wood (HW) and soft wood (SW) sawdusts from Mitragyna tree and Xylopia tree at 600g during the dry (D) and rainy (R) seasons. The sawdusts were supplemented with 100g of rice bran and 1g of Rhizophora wood ash. Eight treatments were established based on the interactions of the species to the substrates and seasons viz: P1SWR, P1HWR, P2SWR, P2HWR, P1SWD, P1HWD, P2SWD and P2HWD. Fruiting potential was assessed based on the mean harvest weight and was done for five weeks. P1HWD recorded highest mean weight (33.11g) during the dry season while P1HWR had highest mean weight (33.54g) for week1 harvest. P2 recorded no harvest at the first week. Highest mean weight of 21.72g and 24.61g were observed for P2SWD and P1SWR respectively for the second week of harvest. Week 3 harvest revealed P1SWD and P2SWR with the highest mean weights (14.05g and 23.07g) respectively. Within the fourth week of harvest, P2SWD (5.34g) and P2HWR (17.36g) recorded highest mean weights compared to other treatments. At the fifth week of harvest, highest mean values (10.67g and 17.04g) were seen for P2SWD and P2HWR respectively. Generally, P1 had more fruiting potential than P2 as its treatments fruited in all the weeks of harvest. Soft wood sawdust during the rainy season favoured higher yield than hardwood sawdust during the dry season.
在美国河流州立大学植物科学与生物技术系对两种平菇(P. ostreatus和P. eryngii)在旱季和雨季的结果进行了研究。两种(P. ostreatus;P1和P. yyngii;P2)分别生长在600g的米特拉金那树和木杉的硬木(HW)和软木(SW)锯末上,分别生长在干旱季节(D)和雨季(R)。在木屑中添加100g米糠和1g根霉木灰。根据植物对基质和季节的相互作用,确定了8个处理:P1SWR、P1HWR、P2SWR、P2HWR、P1SWD、P1HWD、P2SWD和P2HWD。结果潜力是根据平均收获重量评估,并进行了5周。P1HWD在旱季记录了最高的平均重量(33.11g),而P1HWR在第一周收获时记录了最高的平均重量(33.54g)。P2在第一周没有收获。收获第二周,P2SWD和P1SWR的平均体重最高,分别为21.72g和24.61g。第3周收获时,P1SWD和P2SWR的平均体重最高,分别为14.05g和23.07g。在收获的第四周,与其他处理相比,P2SWD (5.34g)和P2HWR (17.36g)的平均体重最高。在收获的第5周,P2SWD和P2HWR的平均值最高,分别为10.67g和17.04g。总体而言,P1处理在收获周内均有结果,结果势大于P2处理。软木锯末在雨季比硬木锯末在旱季有利于更高的产量。
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引用次数: 0
Antifungal Potential of Tetrapleura tetraptera, Xylopia aethiopica, Citrus aurantifolia and Cymbopogon citratus on Different Fungal Isolates 四翅四胸草、青木、金叶柑橘和香茅对不同真菌分离株的抑菌作用
Pub Date : 2023-10-14 DOI: 10.56201/jbgr.v9.no2.2023.pg9.17
W. E. J. Nneji, E. C. Chuku, A. B. Nwauzoma, F. W Nmom
study on the antifungal potentials of various plants extracts on mycoflora of groundnut pod was carried out in the Department of Plant Science and Biotechnology, Rivers State. Cultural laboratory methods was used to evaluate the mycoflora. Powdered plants extracts used were extracted by air drying and blending the leaves of the plants. The antifungal activity on the various fungal isolates was done at different concentrations (100, 50, 25, and 12.5mg/ml). For antifungal activity of C. citratus at 100, 50 and 25mg/ml revealed higher inhibition of Scopulariopsis sp compared to other isolates, although at 12.5mg/ml there was complete inhibition across all fungal isolates. X. aethiopica showed more inhibition for all isolates at the different concentrations while T. tetraptera and C. aurantifolia revealed total inhibition of these isolates at 12.5mg/ml with variations across other concentrations respectively. Generally all plants extract showed complete inhibition at 12.5mg/ml.
河流州立大学植物科学与生物技术系研究了不同植物提取物对花生荚果菌群的抑菌作用。采用培养实验室法对菌群进行鉴定。粉末状植物提取物是通过空气干燥和混合植物的叶子提取。不同浓度(100、50、25和12.5mg/ml)对不同真菌分离物的抑菌活性进行了研究。柠檬酸C. citratus在100mg /ml、50mg /ml和25mg/ml浓度下对Scopulariopsis sp的抑制作用高于其他菌株,而在12.5mg/ml浓度下对所有真菌都有完全抑制作用。在不同浓度下,埃塞俄比亚锥虫对所有菌株均有较强的抑制作用,而在12.5mg/ml浓度下,四翅天牛和金翅天牛对所有菌株均有较强的抑制作用,但在其他浓度下存在差异。在12.5mg/ml浓度下,所有植物提取物均表现出完全抑制作用。
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引用次数: 0
Bacteriological Quality of Selected Sachet Water in Iree Community, Osun State, Nigeria 尼日利亚奥孙州Iree社区选定香袋水的细菌质量
Pub Date : 2023-10-14 DOI: 10.56201/jbgr.v9.no2.2023.pg18.24
S.O. Olanrewaju, E.B. Akinro, A.D Aasa – Sadique, M.A. Abiona, E.A. Aboderin, I.A. Adetuberu, T. Busari
Water is an unaffordable resource and vital for human life. Potable drinking water is a basic need for human’s good health. The aim of the study was to examine the qualities of available sachet drinking water in Iree community bacteriologically. Ten different brands if sachet water were selected and were all purchased within Iree community. The selected samples were examined for Total Heterotrophic Bacterial Count (THBC), Total Coliform Counts (TCC) and Total Thermotolerant (Faecal) Count (TTC) using standard microbiological methods. The THBC values obtained ranged from (1.10 x 10
水是一种负担不起的资源,对人类生活至关重要。饮用水是人类健康的基本需要。本研究的目的是从细菌学的角度对Iree社区可用的小袋饮用水的质量进行检测。我们选择了10个不同品牌的小袋水,并在Iree社区内购买。采用标准微生物学方法检测所选样品的总异养细菌计数(THBC)、总大肠菌群计数(TCC)和总耐热性(粪)计数(TTC)。获得的THBC值范围为(1.10 × 10)
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引用次数: 0
Effect of Methanolic Extract of Ginger on Gastric PH and Serum Bicarbonate of Wistar Rats 姜醇提物对Wistar大鼠胃PH和血清碳酸氢盐的影响
Pub Date : 2023-09-25 DOI: 10.56201/jbgr.v9.no1.2023.pg1.17
Onyebuchi Obia, Winifred C. Udeh
Ginger plant has a perennial, tuberous root or rhizome. The rhizome which is the horizontal stem from which the roots grow, is the main portion of ginger that is consumed as a spice in dried, preserved and green forms. The aim of this study was to determine the effect of methanolic extract of ginger on gastric pH and post-prandial serum bicarbonate in wistar rats. A total of 20 male rats weighing 180 to 250g were divided into four groups of 5 rats each; Group I served as control, Group II received 100mg/kg methanolic ginger extract, Group III received omeprazole (20mg/kg) and Group IV received a combination of omeprazole and methanolic ginger extract. After 45 minutes of administration of the extract, the pH of the gastric effluent increased significantly whereas the post-prandial serum bicarbonate concentration decreased significantly compared to control (p<0.05). Although Omeprazole caused marked reduction in gastric pH, coadministering with methanolic ginger extract prevented excessive reduction in gastric pH that occurred with the drug alone. In the determination of serum post-prandial bicarbonate level, the methanolic ginger extract group showed levels significantly lower than the control group while the Omeprazole and co-administration groups were significantly lower in comparison with extract group. Hence, the study concluded that methanolic ginger extract alone neutralized gastric acid secreted while in combination with Omeprazole, it acted as a buffer to stabilize the gastric pH.
生姜植物有多年生块茎根或根茎。根茎是根生长的水平茎,是生姜的主要部分,以干燥、保存和绿色的形式作为香料食用。本研究旨在探讨生姜醇提物对wistar大鼠胃pH值及餐后血清碳酸氢盐的影响。选取体重180 ~ 250g的雄性大鼠20只,分为4组,每组5只;ⅰ组为对照组,ⅱ组给予甲醇姜提取物100mg/kg,ⅲ组给予奥美拉唑(20mg/kg),ⅳ组给予奥美拉唑与甲醇姜提取物联合治疗。给药45分钟后,与对照组相比,胃流出液pH显著升高,餐后血清碳酸氢盐浓度显著降低(p<0.05)。虽然奥美拉唑引起胃pH值的显著降低,但与甲醇生姜提取物共同使用可以防止单独使用该药时胃pH值的过度降低。在测定血清餐后碳酸氢盐水平时,乙醇生姜提取物组显著低于对照组,奥美拉唑和共给药组显著低于提取物组。因此,本研究认为,生姜甲醇提取物单独中和胃酸分泌,而与奥美拉唑联合使用,则具有稳定胃pH的缓冲作用。
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引用次数: 0
Use of Wastewater from Legume Cooking in Bioremediation of Crude-Oil Polluted Soil 豆科植物蒸煮废水在原油污染土壤生物修复中的应用
Pub Date : 2023-09-25 DOI: 10.56201/jbgr.v9.no1.2023.pg37.53
Lekiah Pedro Peekate
Wastewaters generated from the cooking of foods contain nutrients. Such wastewaters may have the potential to be used as nutrients in bioremediation of hydrocarbon polluted environments. In this study, use of wastewaters generated from the cooking of two legumes as nutrient source during bioremediation of hydrocarbon polluted soil was investigated. Crudeoil polluted soils were treated with wastewater from cooking of cowpea (setup B), wastewater from cooking of groundnut-pods (setup G), a mixture of the wastewaters (setup B+G), and tap-water (control setup: setup C). Selected microbial group and physicochemical parameters were determined at seven days interval. The results obtained showed that the extent of hydrocarbon degradation in setup B, G, B+G, and C were 45.5, 23.2, 1.3, and 37.9 % respectively. There was decrease in total organic carbon (TOC), phosphorus, and nitrogen in the setups. However, in setup G there was increase in TOC. Setup C and B had the highest decrease in phosphorus, and setup B had the highest decrease in nitrogen. There was decrease and subsequent increase in percent hydrocarbon-utilizing bacteria in all the setups; the subsequent increase was highest in setup B. There was generally a decrease in percent hydrocarbon-utilizing fungi, except in setup C. The hydrocarbon utilizing microorganisms isolated from the treated soils include Bacillus, Micrococcus, Acinetobacter, Staphylococcus, Pseudomonas, Penicillium, Aspergillus, Candida and Fusarium. Their presence indicates that hydrocarbon degradation was due to biodegradation. It is concluded that wastewater from cooking of cowpeas can be applied in bioremediation of crude-oil polluted soil.
烹饪食物产生的废水含有营养物质。这些废水可能有潜力用作碳氢化合物污染环境的生物修复中的营养物。以两种豆科植物蒸煮产生的废水为营养源,研究了生物修复烃类污染土壤的方法。采用豇豆蒸煮废水(设置B)、花生豆荚蒸煮废水(设置G)、废水(设置B+G)的混合物和自来水(对照设置C)处理原油污染土壤。每隔7天测定选定的微生物群和理化参数。结果表明,B、G、B+G和C组的烃类降解率分别为45.5%、23.2%、1.3%和37.9%。总有机碳(TOC)、总磷、总氮均有降低。然而,在设置G中TOC有所增加。设置C和B的磷下降幅度最大,设置B的氮下降幅度最大。在所有设置中,利用烃类细菌的百分比都先减少后增加;从处理过的土壤中分离出的利用烃类的微生物有芽孢杆菌、微球菌、不动杆菌、葡萄球菌、假单胞菌、青霉菌、曲霉、念珠菌和镰刀菌。它们的存在表明烃类降解是由生物降解引起的。结果表明,豇豆蒸煮废水可用于原油污染土壤的生物修复。
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引用次数: 0
Application of Optimization Models in Modification of Nutrient- Chloramphenicol Agar Medium for Use in Selective Isolation of Pseudomonasfluorescens. 优化模型在改良氯霉素琼脂培养基中用于荧光假单胞菌选择性分离的应用。
Pub Date : 2023-09-25 DOI: 10.56201/jbgr.v9.no1.2023.pg54.66
Wisdom Dawoye Atemie, Lekiah Pedro Peekate
Commercially available medium for selective isolation of Pseudomonas are relatively expensive. Incorporation of chloramphenicol into nutrient agarhas been used in the selective isolation of Pseudomonasfluorescens. Theaim of this study was to apply optimization in modification of nutrient-chloramphenicol agar medium (NCAM) so as to optimize it for selective isolation of Pseudomonas species. Water sample determined to contain fluorescent greenish-pigment producing bacteria (FGPB) was used for the optimization study. Medium component selected for the optimization process were pH, NaCl concentration (NC), and chloramphenicol concentration (CC), with value ranges of 7.0 – 8.2, 0.5 – 1.0 %, and 40 – 60 ?g/ml, respectively. Fifteen media variations were derived from combination of these ranges using Box-Behnken design matrix,and were used in culturing the water sample for isolation of FGPB. Relationship between obtained proportions of bacterial populations that are FGPB and chosen media components were calculated using polynomial equation for three factors design. Prediction profiles derived from the equation showed that the highest proportion of bacterial populations that are FGPB was achievable at pH, NC, and CC combination values of 7.0, 0.2 %, and 60 ?g/ml, respectively. NCAM with this optimized combination increased the efficiency of selectively isolating FGPB from the water samplefrom 0.008 to 0.011 %. Physicochemical/biochemical testing showed that the FGPBisolates were Pseudomonas fluorescens. It is concluded that NCAM with pH 7.0, NaCl concentration of 0.2 %, and chloramphenicol concentration of 60 ?g/ml can be used for selective isolation of Pseudomonas fluorescens with optimum efficiency.
市售的选择性分离假单胞菌的培养基相对昂贵。将氯霉素掺入营养琼脂中用于荧光假单胞菌的选择性分离。本研究的目的是将优化技术应用于营养物氯霉素琼脂培养基(NCAM)的修饰,以优化其对假单胞菌的选择性分离。以检测到的水样中含有荧光绿色素产生菌(FGPB)进行优化研究。优化工艺选用pH、NaCl浓度(NC)和氯霉素浓度(CC),分别为7.0 ~ 8.2、0.5 ~ 1.0%和40 ~ 60g /ml。使用Box-Behnken设计矩阵从这些范围的组合中得出15种培养基变化,并用于培养水样以分离FGPB。采用三因素设计的多项式方程计算所得的FGPB菌群比例与所选培养基组分之间的关系。由该方程得出的预测曲线显示,当pH、NC和CC组合值分别为7.0、0.2%和60g /ml时,FGPB细菌群体的比例最高。该优化组合的NCAM将水样中FGPB的选择性分离效率从0.008%提高到0.011%。理化/生化检测表明,分离的fgpb为荧光假单胞菌。结果表明,在pH 7.0、NaCl浓度0.2%、氯霉素浓度为60 ?g/ml的条件下,NCAM对荧光假单胞菌的选择性分离效果最佳。
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引用次数: 0
Ethno-Medicinal Plants Used for Respiratory Disorders among the Igala and Yoruba (Okun) People in Kogi State, Nigeria 尼日利亚科吉州伊加拉和约鲁巴(奥肯)人用于呼吸系统疾病的民族药用植物
Pub Date : 2023-09-25 DOI: 10.56201/jbgr.v9.no1.2023.pg18.36
Owa Frank Dele, Aniama Salome Ojone
Respiratory disorders are common problem and cause of death among the Igala and Yoruba (Okun) speaking people of Kogi State due to climatic conditions and scarce health care facilities. The people rely upon the indigenous plant resources to cure various respiratory disorders. The focus for this present study is to document available ethno-medicinal data of plants used for respiratory disorders among the Igala and Yoruba (Okun) speaking people of Kogi State. A total of 84 species belonging to 42 families used to treat respiratory disorders among the Igala and Okun people has been documented. Cough was the disorder treated by the highest number of species followed by asthma, bronchitis, cold, Tonsillitis, Convulsion, Throat infections, Tuberculosis, Epilepsy, Catarrh and then Hiccup respectively. The common plant parts and preparation methods of the medicinal recipes are from leaves, root, seeds, fruit, stem, bark, the whole plant, flower, rhizome, bulb and corm and latex in the form of infusion, decoction, extracts, pastes, juice and or chewed. A sample of 900 respondents from the study areas who have wealth of knowledge in plant use and traditional medicine were drawn using a stratified random sampling technique. A well organized questionnaire was the major source of instrument for data collection. Part of plants and their mode of application will also be sought. It is recommended that detailed phytochemical studies be carried out to ascertain the active ingredients.
由于气候条件和缺乏保健设施,呼吸系统疾病是科吉州说伊加拉和约鲁巴语(奥昆语)人的常见问题和死亡原因。人们依靠当地的植物资源来治疗各种呼吸系统疾病。本研究的重点是记录科吉州讲伊加拉和约鲁巴(奥昆语)的人用于呼吸系统疾病的植物的现有民族药物数据。已记录在案的用于治疗Igala和Okun人呼吸系统疾病的42科共84种。治疗种类最多的疾病是咳嗽,其次是哮喘、支气管炎、感冒、扁桃体炎、惊厥、咽喉感染、肺结核、癫痫、黏膜炎和呃嗝。常用的植物部位和制剂方法为叶、根、种子、果实、茎、树皮、整株、花、根茎、球茎、球茎和乳胶,以浸渍、煎煮、提取物、膏体、果汁和(或)咀嚼的形式。采用分层随机抽样技术抽取了来自研究地区的900名答复者样本,这些人在植物利用和传统医学方面拥有丰富的知识。一份组织良好的调查表是收集数据的主要工具来源。还将寻求部分植物及其应用方式。建议进行详细的植物化学研究以确定其有效成分。
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