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Evaluation of propagation of Bougainvillea (Bougainvillea glabra) under different plantation conditions 不同种植条件下九重葛(Bougainvillea glabra)的繁殖评估
Pub Date : 2024-06-14 DOI: 10.38211/joarps.2024.05.262
Mohammad Asif, Amir Ali, Khalil Ahmed, Qamber Khan, Asif Irshad, Musaddiq Khalid, Afifa Talpur, Safdar Ali Wahocho, N. A. Wahocho
Bougainvillea is popularly used as an ornamental plant, appraised because of its aesthetic bracts, but it undergoes difficulty in propagation due to incompetence in root growth. Hence comprehensive research is required for improving its propagation ways to widen its cultivation. The present study was therefore conducted to explore the growth, rooting, and sprouting response of Bougainvillea cuttings to different planting conditions (tunnel and open field conditions). The research was executed by applying a randomized complete block design with factorial arrangement. Cuttings of two bougainvillea types (Climbing and Bush Bougainvillea) were used. It was noted that growth and rooting of both bougainvillea types were significantly (P<0.05) affected by different plantation conditions. The results elaborated that the Bougainvillea cuttings planted under tunnel conditions took less time to sprouting (5.50) with maximum branches (20.01), sprouting (88.33%) and survivability (83.33 %). The cuttings grown under tunnel conditions also exhibited maximum shoot and root biomass production (15.46 g and 1.15 g) and root numbers (12.68). Concerning the Bougainvillea types, "Bush bougainvillea" showed supervisor performance for most of the assessed traits in comparison with its counterpart “Climbing bougainvillea”. After reviewing the findings of this study, it is determined that bougainvillea plantation with plastic tunnels had better results in terms of sprouts, rooting and growth characteristics. Pertaining to bougainvillea types, the "Bush bougainvillea” exhibited greater results than the "Climbing bougainvillea".
九重葛是一种广受欢迎的观赏植物,因其美丽的苞片而备受赞誉,但由于根系生长不健全,其繁殖十分困难。因此,需要进行全面研究,改进其繁殖方法,扩大其种植范围。因此,本研究旨在探索九重葛插条在不同种植条件(隧道和露地条件)下的生长、生根和萌芽反应。研究采用了因子排列的随机完全区组设计。研究使用了两种九重葛类型(攀缘九重葛和丛生九重葛)的插条。结果表明,两种九重葛的生长和生根都受到不同种植条件的显著影响(P<0.05)。结果表明,在隧道条件下种植的九重葛插条萌芽时间较短(5.50),分枝最多(20.01),萌芽率最高(88.33%),存活率最高(83.33%)。在隧道条件下生长的插条还表现出最大的嫩枝和根生物量产量(15.46 克和 1.15 克)和根数(12.68)。在九重葛类型方面,"灌木九重葛 "与 "攀缘九重葛 "相比,在大多数评估性状上表现出更优越的性能。研究结果表明,使用塑料隧道种植的九重葛在萌芽、生根和生长特性方面效果更好。在九重葛类型方面,"灌木九重葛 "比 "攀缘九重葛 "的效果更好。
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引用次数: 0
Phenotypic and Genotypic Screening of Green Super Rice Genotypes for Submergence Tolerance at Seedling Stage 苗期绿色超级稻基因型耐淹水性的表型和基因型筛选
Pub Date : 2024-06-13 DOI: 10.38211/joarps.2024.05.277
Faiza Siddique, Abid Majeed, Q. Sani, M. Farooq, Rehana Kausar, M. Uzair, Atif Naeem, Mashal Rehman, Shafiq Ahmed, Maria Rabnawaz
Climate change threatens rice-based systems, especially in areas where crops are sensitive to temperature fluctuations. Rice production is significantly impacted by extreme weather events, and persistent and heavy rainfalls which cause floods lead to submergence stress. The primary focus of this research was the evaluation of rice genotypes under controlled flooding conditions. The study included nine green super rice lines and one local control variety based on better tolerance against submergence stress. Using genotyping based on SSR markers, the goal was to better understand how rice germplasm responded to submergence stress at the seedling stage. The treatments included: T0, the control without submergence stress; T1, submergence for 10 days with ethylene treatment; and T2, submergence for 10 days without ethylene treatment. Ten genotypes were tested under complete seedling submergence. Submergence-tolerant genotypes were identified using the RM23877 SSR marker for genotyping. The results highlighted significant genotypic variations (alleles and genetic markers associated with submergence tolerance) in response to submergence stress affected by ethylene treatment, with variable effects observed for different genotypes. Most genotypes had zero survival except for GSR-4 and GSR-61, followed by GSR-5, GSR-13, GSR-2, and Chenab basmati. SSR marker-based genotyping further revealed that six out of ten genotypes present the submergence tolerance allele. We observed significant genotypic variations in the alleles associated with submergence tolerance, including differences in the Sub1A-1, Sub1B, and Sub1C genes among the GSR genotypes. These findings lay the groundwork for marker-assisted selection in breeding programs to develop rice varieties with enhanced submergence tolerance.
气候变化威胁着以水稻为基础的系统,尤其是在作物对温度波动敏感的地区。水稻生产受到极端天气事件的严重影响,持续的强降雨导致洪水泛滥,造成淹没胁迫。这项研究的主要重点是评估受控洪涝条件下的水稻基因型。研究对象包括九个绿色超级稻品系和一个当地对照品种,这些品系对淹没胁迫具有更好的耐受性。利用基于 SSR 标记的基因分型,目的是更好地了解水稻种质在幼苗阶段如何应对淹没胁迫。处理包括T0,无浸没胁迫的对照;T1,浸没 10 天并进行乙烯处理;T2,浸没 10 天并不进行乙烯处理。在完全浸没幼苗的情况下,对 10 个基因型进行了测试。利用 RM23877 SSR 标记进行基因分型,确定了耐淹没基因型。结果表明,在乙烯处理的影响下,不同基因型对淹没胁迫的反应存在明显的基因型差异(与耐淹没性相关的等位基因和遗传标记),并观察到不同基因型的不同影响。除 GSR-4 和 GSR-61 外,大多数基因型的存活率为零,其次是 GSR-5、GSR-13、GSR-2 和 Chenab basmati。基于 SSR 标记的基因分型进一步显示,10 个基因型中有 6 个存在耐淹没等位基因。我们观察到与耐淹性相关的等位基因存在明显的基因型差异,包括 GSR 基因型中 Sub1A-1、Sub1B 和 Sub1C 基因的差异。这些发现为育种计划中的标记辅助选择奠定了基础,以培育出具有更强耐淹性的水稻品种。
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引用次数: 0
Impact of Humic Acid on the Morphological Components and Growth Parameters of Wheat (Triticum Aestivum L.) Under Dry Climate of Uthal 腐植酸对乌塔尔干旱气候下小麦形态成分和生长参数的影响
Pub Date : 2024-06-09 DOI: 10.38211/joarps.2024.05.244
Siraj Ahmed, S. Kalhoro, Bilal Ahmed, K. A. Kubar, Mehar-un-Nisa Narejo, Qamar Sarfaraz, Muneer Ahmed Roden, Khalid Hameed, Shabir ahmed, Sami Ullah, Sher Jan
Humic acid is natural biological organic, which has a high effect on plant growth and quality. However, the mechanisms of the promoting effect of humic acid on the morphological components, growth and yield parameters were rarely reported. In this study, the effects of soil application of humic acid on the morphological components and growth parameters of wheat under dry climate were explored. Randomized complete block design (RCBD) with five treatments of humic acid and replicated three time, T1=control, T2= 4.5kgha-1, T3= 6 kgha-1, T4= 9.5 kgha-1, T5= 12 kgha-1 and T6= 14.5 kgha-1. Findings of this study indicated that with increasing application of humic acid growth and yield also increases plant height (21.97%), spike weight (157%), leaf area (34.12%), grain spike-1 (93%), grain yield (49.36%) and biological yield (80.34), though pH of soil also increased. While; results of NO3-N and K were also significantly different under different level of humic acid and mean maximum were recorded in T6 (25% and 48%) in comparison of T1, T2, T3, T4 and T5. Conclusively; this study revealed that application of humic acid at the rate of 14.5 kg ha-1 could improve the growth and yield parameters of wheat and soil fertility.
腐植酸是天然的生物有机物,对植物的生长和品质有很高的影响。然而,腐植酸对小麦形态成分、生长和产量参数的促进作用机制却鲜有报道。本研究探讨了在干旱气候条件下,土壤施用腐植酸对小麦形态成分和生长参数的影响。研究采用随机完全区组设计(RCBD),共设五个腐植酸处理,重复三次,T1=对照,T2=4.5kgha-1,T3=6 kgha-1,T4=9.5 kgha-1,T5=12 kgha-1,T6=14.5 kgha-1。研究结果表明,随着腐植酸施用量的增加,株高(21.97%)、穗重(157%)、叶面积(34.12%)、穗粒数-1(93%)、谷物产量(49.36%)和生物产量(80.34%)也随之增加,但土壤的 pH 值也随之增加。与 T1、T2、T3、T4 和 T5 相比,NO3-N 和 K 的结果在不同腐殖酸水平下也有显著差异,平均最大值出现在 T6(25% 和 48%)。总之,这项研究表明,以 14.5 kg ha-1 的比例施用腐植酸可以提高小麦的生长和产量参数以及土壤肥力。
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引用次数: 0
Boosting Peach Production in Rainfed Areas of Punjab Through Mulching Techniques 通过覆盖技术提高旁遮普雨浇地区的桃子产量
Pub Date : 2024-06-06 DOI: 10.38211/joarps.2024.05.263
M. Yunas, Majid Rahim, R. Bibi, A. Aulakh, W. Naseem, S. Malik, Sultan Ahmad Rizvi, Obaid-ur Rehman
Mulching is an approach to cover soil around plants with materials like polyethylene sheets, organic matter or crop residues, gaining wide recognition for its capability to enhance soil moisture retention, regulate soil temperature and suppress weed growth. The experiment was conducted at Soil and Water Conservation Research Institute, Chakwal aimed at investigating the potential of mulching techniques to enhance peach production in rainfed areas of Punjab. The treatments were laid out in Randomized Complete Block Design (RCBD), selecting two years old peach plants. The mulch treatments were polypropylene sheet, black plastic polyethene sheet, grass, gypsum amendment, and control (without any mulch). The results indicated a substantial improvement in mean soil moisture content, with the maximum enhancement observed in the treatment utilizing black sheet mulch (10.8% and 11.2%) followed closely by polypropylene sheet mulch (10% and 10.1%) in 1st and 2nd year respectively. Fruit yield, a critical parameter for assessing orchard productivity, exhibited the highest values in treatment unit comprising black sheet mulch, recording an impressive 27.2 kg plant-1. Conversely, the control showed the lowest fruit yield as 18.5 kg plant-1. These findings emphasize the significant potential of mulching techniques in optimizing soil moisture levels and augmenting peach fruit yield in rainfed areas. The study contributes into sustainable agricultural practices, illustrating the practical benefits of mulching strategies to improve water retention and overall orchard productivity in rain fed areas. This study will be helpful for policymakers, farmers, and researchers for adopting this technique in peach cultivation in rainfed areas of Punjab.
地膜覆盖是一种用聚乙烯板、有机物或作物残茬等材料覆盖植物周围土壤的方法,因其能够提高土壤保墒能力、调节土壤温度和抑制杂草生长而得到广泛认可。该实验在查克瓦尔水土保持研究所进行,旨在研究覆盖技术在提高旁遮普雨浇地区桃子产量方面的潜力。试验采用随机完全区组设计(RCBD),选择两年生的桃株进行处理。地膜覆盖处理包括聚丙烯薄膜、黑色塑料聚乙烯薄膜、草、石膏改良剂和对照(无任何地膜覆盖)。结果表明,在第一年和第二年,平均土壤含水量有了显著提高,其中使用黑色塑料薄膜覆盖的处理(10.8% 和 11.2%)提高幅度最大,紧随其后的是聚丙烯薄膜覆盖(10% 和 10.1%)。果实产量是评估果园生产率的关键参数,在使用黑色地膜覆盖的处理单元中,果实产量最高,达到了令人印象深刻的 27.2 公斤/株。相反,对照组的果实产量最低,为 18.5 千克/株。这些发现强调了地膜覆盖技术在优化土壤水分水平和提高雨养地区桃子产量方面的巨大潜力。这项研究为可持续农业实践做出了贡献,说明了在雨水灌溉地区采用覆盖策略提高保水能力和果园总体产量的实际好处。这项研究将有助于政策制定者、农民和研究人员在旁遮普雨水灌溉地区的桃子种植中采用这种技术。
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引用次数: 0
Rock Phosphate and Manures with Phosphorus Solubilizing Bacteria Increases the Growth, Yield and Phosphorus Uptake of Okra (Abelmoschus esculentus) 磷矿石和含有磷溶解细菌的肥料可提高秋葵(Abelmoschus esculentus)的生长、产量和磷吸收率
Pub Date : 2023-12-21 DOI: 10.38211/joarps.2024.05.226
Nasir Rahim, Zaheer Yasin, M. M. Tahir, Afshan Majeed, Abid Yaqub, Basharat Mahmood
Phosphorus (P) is one of the most important plant nutrient for increasing soil productivity and sustainable crop production. A pot experiment was conducted to study the effect of seed inoculation by phosphorus solubilizing bacteria (PSB) and organic and mineral P fertilizers on growth, yield and P uptake of okra. Phosphorus was applied at the rate of 90 kg ha-1 in the form of poultry manure (PM); sheep manure (SM) and rock phosphate (RP). Nine treatments were allocated for the experiment having PM, SM and RP alone, organic manures with RP in combination, or a mix of the three with PSB. The result revealed that sole application of SM at three growth stages gave maximum root and shoot growth, which was 75 and 78% more from control respectively. It is concluded that Leaf area (36%), chlorophyll contents (62%), yield (60%) and P uptake (31%) of okra were significantly enhanced by the integrated use of PM+SM+RP+PSB at all growth stages over control. Complementary application of this inoculants with manures and RP most favored okra growth and yield and can be considered as an appropriate substitute for chemical P fertilizer in organic and sustainable agricultural system.
磷(P)是提高土壤生产力和作物可持续生产最重要的植物养分之一。为了研究磷溶解菌(PSB)种子接种以及有机和矿物磷肥对秋葵生长、产量和磷吸收的影响,我们进行了一项盆栽实验。磷肥的施用量为每公顷 90 千克,分别以家禽粪便(PM)、羊粪(SM)和磷矿石(RP)的形式施用。试验共分配了 9 个处理,分别是单独施用磷肥、羊粪和磷酸盐,有机肥与磷酸盐混合施用,或将这三种肥料与聚苯乙烯混合施用。结果表明,在三个生长阶段单独施用 SM 可使根系和芽的生长量最大,分别比对照组高出 75% 和 78%。结论是,在所有生长阶段综合使用 PM+SM+RP+PSB,秋葵的叶面积(36%)、叶绿素含量(62%)、产量(60%)和钾吸收量(31%)都比对照组显著提高。在有机和可持续农业系统中,这种接种剂与粪肥和 RP 的互补施用最有利于秋葵的生长和产量,可被视为化学磷肥的适当替代品。
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引用次数: 0
Integrated Effect of Organic Amendments and Chemical Fertilizers on yield of Groundnut and Soil Health Under Rainfed Condition 有机添加剂和化肥对雨水灌溉条件下花生产量和土壤健康的综合影响
Pub Date : 2023-12-20 DOI: 10.38211/joarps.2024.05.215
Muhammad Arsalan, Abdul Latif, Madeehaa Khan, Sairah Syed, Rehmat Ullah, Ijaz Ahmad, Muhammad Bilal, Muhammad Tariq Mahmood, Muhammad Ehsan, Rizwan Latif, Abdul Ghaffar, Sanaullah
Although inorganic fertilizers increase the growth of crops, but their toxicity is the main concern due to their over utilization. To tackle this problem, the integrated application of organic amendment especially vermicompost, with NPK fertilizers would be a good choice. For this, the integrated effect of organic amendments including vermicompost (VC) and phosphorus solubilizing bacteria (PSB), and chemical fertilizers (NPK) on yield of groundnut and soil health, a field trial was done at the research area of Barani Agricultural Research Institute (BARI), Chakwal. The experiment was done with eight treatments in randomized complete block factorial design replicated thrice. The experimental data revealed that the treatment T8: Recommended dose of fertilizers (RDF) (NPK 20:80:60 Kg ha-1) + VC @ 4t ha-1 + PSB had superior effect on plant height (54.76.23%), number of plants per m2 (22.25%), number of pods per plant (61.90%), pod yield (83.25%) and haulem yield (86.02%) as compared to control. Similarly, a higher benefit cost ratio (BCR) was observed in T8 than rest of the treatments and control. Soil fertility indicators (OM, NO3-N, P and K) were improved, indicating the improvement in soil health with the combine application of VC, PSB and RDF. A combination of organic amendment, bio fertilizer, and inorganic fertilizer is necessary to optimize groundnut growth and yield attributes, as well as soil health.
虽然无机肥料能促进作物生长,但由于过度使用,无机肥料的毒性成为人们关注的主要问题。为解决这一问题,有机肥料(尤其是蛭肥料)与氮磷钾肥料的综合施用将是一个不错的选择。为此,在 Chakwal 的 Barani 农业研究所(BARI)研究区进行了一项田间试验,研究有机肥料(包括蛭石堆肥(VC)和磷溶解菌(PSB))与化肥(NPK)对花生产量和土壤健康的综合影响。试验采用随机完全区组因子设计,共设八个处理,重复三次。实验数据显示,与对照相比,处理 T8:推荐剂量肥料(RDF)(NPK 20:80:60 Kg ha-1)+VC @ 4t ha-1+PSB 对株高(54.76.23%)、每平方米株数(22.25%)、每株荚数(61.90%)、荚产量(83.25%)和胡麻产量(86.02%)的影响更佳。同样,T8 的效益成本比(BCR)也高于其他处理和对照。土壤肥力指标(OM、NO3-N、P 和 K)均有所改善,表明结合施用 VC、PSB 和 RDF 改善了土壤健康。有必要将有机肥、生物肥和无机肥结合起来,以优化花生的生长和产量属性以及土壤健康。
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引用次数: 0
Effect of Foliar Application of Phosphorus and Zinc on Biometric and Quality Attributes of Fodder Maize in Calcareous Saline-Sodic Soils 叶面施肥磷和锌对石灰性盐碱地饲料玉米生物计量和质量属性的影响
Pub Date : 2023-12-20 DOI: 10.38211/joarps.2024.05.220
Muhammad Amjad Bashir, A. Rehim, Namra Khurshid, Qurat-Ul-Ain Raza, Hifsa Khurshid, Hafiz M. U. Raza
The hot climate is the major reason to promote salt salinization and sodication, which retards the crop productivity. The increase in salt-affected soils is adversely affecting worldwide productivity. The antagonistic effect among P and Zn causes nutrient deficiency and increases under saline conditions. The present study aimed to identify the targeted influence of foliar application of P and Zn on maize biometric and fodder quality parameters under saline-sodic conditions. The experiment was based on three P (0, 1, 2, 2.5%) and Zn levels (0, 1, and 1.5%), with three replications. The study showed that P concentration in maize was improved significantly with Z1P1 (51.0%) application, followed by Z0P2 (33.15%) and Z1.5P2.5 (28.0%). The Zn concentration enhanced with Z1P0 (91.73%), followed by Z0P1 (84.45%) and Z1.5P1 (84.18%). Nitrogen concentration improved with Z1.5P2.5 (39.84%). Total mineral contents were increased with Z1.5P2 (156.71%), followed by Z0P1 (142.64%) and Z0P2 (141.99%). Crude protein concentration was improved in Z1.5P1 (39.92%), followed by Z0P2 (11.92%). Crude fat percentage was increased with Z0P2.5 (51.89%), followed by Z0P1 (34.91%) as compared to Z0P0. The study concludes that foliar application of P and Zn in saline-sodic conditions helps retard the negative impacts of salts on biometric and quality parameters of maize fodder.
炎热的气候是导致盐碱化和钠化的主要原因,从而降低了作物的产量。盐渍化土壤的增加对全球生产力产生了不利影响。在盐碱条件下,磷和锌之间的拮抗作用导致养分缺乏和增加。本研究旨在确定在盐碱条件下叶面喷施磷和锌对玉米生物计量和饲料质量参数的针对性影响。实验采用三种钾(0、1、2、2.5%)和锌(0、1、1.5%)水平,三次重复。研究表明,施用 Z1P1(51.0%)能显著提高玉米的钾浓度,其次是 Z0P2(33.15%)和 Z1.5P2.5(28.0%)。施用 Z1P0(91.73%)提高了锌浓度,其次是 Z0P1(84.45%)和 Z1.5P1(84.18%)。Z1.5P2.5 提高了氮浓度(39.84%)。Z1.5P2 增加了总矿物质含量(156.71%),其次是 Z0P1(142.64%)和 Z0P2(141.99%)。Z1.5P1 的粗蛋白浓度有所提高(39.92%),其次是 Z0P2(11.92%)。与 Z0P0 相比,Z0P2.5(51.89%)提高了粗脂肪百分比,Z0P1(34.91%)次之。研究得出结论,在盐碱条件下叶面施肥 P 和 Zn 有助于减缓盐分对玉米饲料生物计量和质量参数的负面影响。
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引用次数: 0
Evaluation of Fruit Bunch Bagging Techniques for Improvement of Loquat Fruit Quality 评估果实捆扎套袋技术以提高枇杷果实质量
Pub Date : 2023-12-19 DOI: 10.38211/joarps.2024.05.212
Tanveer Hussain, Irfan Ali, Ijaz Ahmad, M. Liaquat, Muhammad Tahir Akram, Adeel Anwar, Ghazal Rahim, Sana Asghar, Waqas Naseem, Aysha Manzoor, Faheem Khadija, Muhammad Saqib Naveed, Imran Ali
China is the leading country in the loquat (Eriobotrya japonica L.) production followed by Spain and Japan. In Pakistan it is mainly cultivated in two provinces i.e. Khyber Pakhtunkhwa and Punjab. Present study is aimed to standardize bagging techniques for loquat fruit to enhance quality. Five treatments such as control (unbagged), magazine paper bags, zip locked perforated plastic bags, Chinese paper bags and butter paper bags were used with three replications. Data were recorded for various parameters, including fruit firmness, fruit color, fruit size, fruit and seed weight, ascorbic acid (mg/100g), titratable acidity (%), TSS (°Brix), and reducing and non-reducing sugars. All treatments showed a significant effect on the differences in the evaluated parameters. It was observed that fruit size, fruit weight and ascorbic acid were enhanced when using magazine paper bags (MPB), while fruit firmness, total soluble solids and skin color showed better results in loquat fruits wrapped with zip-locked perforated plastic bags (ZLPPB) compared to the control (unwrapped fruit). Among the bagging treatments, it was revealed that titratable acidity was highest in butter paper bags. RCBD was used for this study. All the readings were subjected to ANOVA and LSD Test (Least Significant Difference Test) for comparison at a 95% level of confidence. In conclusion, fruit bagging at the early developmental stage of fruit in loquat plants with zip-locked perforated plastic bags (ZLPPB) and magazine paper bags (MPB) can be a good choice to enhace the quality of loquat fruit.
中国是枇杷(Eriobotrya japonica L. )的主要生产国,其次是西班牙和日本。巴基斯坦主要在开伯尔-普赫图赫瓦省和旁遮普省种植枇杷。本研究旨在规范枇杷果实的套袋技术,以提高质量。研究采用了五种处理方法,如对照组(未套袋)、杂志纸袋、拉链锁孔塑料袋、中式纸袋和黄油纸袋,三次重复。记录了各种参数的数据,包括果实硬度、果实颜色、果实大小、果实和种子重量、抗坏血酸(毫克/100 克)、可滴定酸度(%)、总糖度(°Brix)、还原糖和非还原糖。所有处理对评价参数的差异都有显著影响。据观察,使用杂志纸袋(MPB)时,果实大小、果实重量和抗坏血酸都有所提高,而与对照组(未包装果实)相比,使用拉锁穿孔塑料袋(ZLPPB)包装的枇杷果实在果实紧实度、总可溶性固形物和果皮颜色方面表现更好。在不同的套袋处理中,黄油纸袋的可滴定酸度最高。本研究使用的是 RCBD。所有读数均采用方差分析和 LSD 检验(最小显著差异检验)进行比较,置信度为 95%。总之,在枇杷果实的早期发育阶段使用拉链穿孔塑料袋(ZLPPB)和杂志纸袋(MPB)进行果实套袋是提高枇杷果实品质的一个不错的选择。
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引用次数: 0
Screening the Soybean Varietal Lines for Selecting High-Yielding and Better Agronomic Traits Producing lines 筛选大豆品系,选育高产和农艺性状更佳的品系
Pub Date : 2023-12-15 DOI: 10.38211/joarps.2024.05.238
Khan Muhammad Zangejo, M. H. Sirohi, Ghulam Raza
Soybean (Glycine max), an economically significant legume originating from East Asia, serves as a valuable source of protein and oil globally. In Pakistan, soybean is considered a non-conventional oilseed crop, with successful cultivation possible during both the spring and summer seasons. The careful selection of appropriate varieties is a primary concern for soybean growers in the country. Therefore, experiments were conducted utilizing the newly developed varietal line in the agroecological zone of Tandojam to select the most suitable and adaptable varieties. A total of forty-six newly developed varietal lines, which have not yet been released, were evaluated alongside four released varieties (Ajmeri, Faisal, Jack, and Rawal) that are already being cultivated in various regions of the country. The field experiments were carried out at the experimental area of the Nuclear Institute of Agriculture (NIA) in Tando Jam during the spring and summer seasons of 2018. Agronomic traits were recorded for each variety, and weather data were obtained from the Pakistan Meteorological Department. All agricultural practices were followed. The results indicate significant variations among the genotypes for all evaluated characteristics. Based on grain production, NIBGE32 exhibited the highest performance, achieving a notable yield of 22g during the summer season, which was 30% higher than the yield of the top-performing control variety, Faisal. Other newly developed varietal lines, namely NIBGE 18, NIBGE 8, NIBGE 45, and NIBGE 41, also demonstrated promising high-yield potential. These varieties displayed comparatively superior growth and yield outcomes. Germination percentage significantly vary in the spring and summer season (Mann-Whitney U =7925, P<0.01); whereas days to maturity, plant height, leaf area, lowest pod height, 100-grain weight, biological yield, seed weight per plant, were not significantly different in two seasons. This suggests a potential for these varietal lines to be cultivated in both seasons. This research endeavours to offer valuable insights and recommendations to farmers and policymakers, aiming to augment soybean production and advance agricultural sustainability in the region of Sindh.
大豆(Glycine max)是源自东亚的一种具有重要经济价值的豆科植物,是全球蛋白质和油脂的重要来源。在巴基斯坦,大豆被认为是一种非常规油籽作物,在春季和夏季均可成功种植。仔细选择合适的品种是该国大豆种植者最关心的问题。因此,在坦多贾姆农业生态区利用新开发的品种系进行了试验,以选出最合适、适应性最强的品种。共有 46 个新开发的品种系尚未发布,与已发布的 4 个品种(Ajmeri、Faisal、Jack 和 Rawal)一起进行了评估。田间试验于 2018 年春夏两季在坦多贾姆的核农业研究所(NIA)实验区进行。记录了每个品种的农艺性状,并从巴基斯坦气象局获得了天气数据。所有农业实践均得到遵循。结果表明,各基因型在所有评估特征方面均存在显著差异。从谷物产量来看,NIBGE32 表现最高,在夏季显著增产 22 克,比表现最好的对照品种 Faisal 的产量高出 30%。其他新开发的品种系,即 NIBGE 18、NIBGE 8、NIBGE 45 和 NIBGE 41,也表现出良好的高产潜力。这些品种的长势和产量都相对较好。发芽率在春夏两季有显著差异(Mann-Whitney U =7925,P<0.01);而成熟天数、株高、叶面积、最低荚高、百粒重、生物产量、单株种子重量在两季没有显著差异。这表明这些品种具有在两季栽培的潜力。本研究旨在为农民和政策制定者提供有价值的见解和建议,以提高信德省地区的大豆产量,促进农业可持续发展。
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引用次数: 0
Purification and Characterization of Alkaline Protease Isolated from Cotton (Gossypium hirsutum) Seeds 棉花种子碱性蛋白酶的纯化及特性研究
Pub Date : 2023-10-25 DOI: 10.38211/joarps.2024.05.201
Asghar Ali Shaikh, Muhammad Umer Dahot, Abdul Sajid, Syed Habib Ahmed Naqvi
Proteases are widely utilized both in physiological and commercial fields such as medicine, food, detergent, and leather. Plant-originated proteases play a significant role in several biomedical fields due to their easy accessibility and activity. Pakistan is an agro-based country and can be an ideal place for the isolation of industrially important proteases from plant sources such as cotton, which is the main crop and frequently available and low cost. Purification of protease was carried out by fractionation with two-fold acetone, ethanol, methanol and various concentrations (40-80%) of ammonium sulphate. The precipitates formed were collected after centrifugation and dialyzed for 24 hours against universal buffer pH 7.0 and was centrifuged in a cooled refrigerated. The dialyzed sample was loaded on Sephadex G–100 gel column. The fractions of the samples were collected and their absorbance of protein was monitored at 280 nm. The homogeneity of the purified enzyme was checked by SDS gel electrophoresis The purified protease enzyme has optimum activity at 30°C and pH 8.0 when casein was used as substrate. The Km and Vmax values of purified cotton seed's alkaline protease activity was recorded as 0.03M and 17 μmol/minute respectively. Protease activity was increased by the addition of cysteine but inhibited by Iodoacetic acid and β-Mercaptoethanol and decreased with some metal ions. These characteristics of the purified enzyme allowed classifying it as a cysteine protease. In conclusion, this study suggests that the alkaline protease enzyme is the best choice for commercial use
蛋白酶在医药、食品、洗涤剂、皮革等生理和商业领域都有广泛的应用。植物源性蛋白酶因其易获得性和活性而在多个生物医学领域发挥着重要作用。巴基斯坦是一个以农业为基础的国家,可以成为从棉花等植物中分离工业上重要蛋白酶的理想场所,棉花是主要作物,经常可用且成本低。用二次丙酮、乙醇、甲醇和不同浓度(40-80%)的硫酸铵分馏提纯蛋白酶。离心后收集形成的沉淀物,在通用缓冲液pH 7.0中透析24小时,在冷却的冰箱中离心。透析后的样品装在Sephadex G-100凝胶柱上。收集样品的组分,在280 nm处监测其对蛋白质的吸光度。以酪蛋白为底物,纯化后的蛋白酶在30℃、pH 8.0条件下活性最佳。纯化棉籽碱性蛋白酶活性Km和Vmax分别为0.03M和17 μmol/min。半胱氨酸能提高蛋白酶活性,碘乙酸和β-巯基乙醇能抑制蛋白酶活性,部分金属离子能降低蛋白酶活性。纯化酶的这些特性使其可以归类为半胱氨酸蛋白酶。综上所述,本研究表明碱性蛋白酶是商业用途的最佳选择
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Journal of applied research in plant sciences
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