A. H. Moubasher, M. Ismail, R. A. Mohamed, Osama Abdel-Hafeez, Mohamed Al-Bedak
Aspergillus flavus AUMC 10331 and A. oryzae AUMC 10329 were used in consortium culture for the production of xylanase enzyme from rice husk using solid-state fermentation technique. The xylanase purification was performed using ion exchange resin IR-120 EP and Sephadex G-75. The purified xylanase showed a total activity of 293.0 IU and a specific activity of 350.96 IU/mg protein and the enzyme was purified to 8.1 fold with 2.7% recovery. The purified xylanase was active over a wide spectrum of pHs from 3-10 and the highest activity was obtained at pH 7.0 followed by 74% of xylanase activity at pH 9.5. At pH 9.5, the xylanase exhibited its optimal activity at 70 °C indicating that the xylanase was alkaliphilic and thermophilic xylanase. The xylanase activity was greatly increased by FeSO4 and CuSO4 up to 332.15% and 194.1% respectively and slightly inhibited by CoCl2. Km and Vmax for the purified xylanase were determined at pH 9.5 and 70 °C for birchwood xylan as 22.13 mg/ml and 135.13 IU/min respectively. The crude and purified enzyme showed high specificity towards the xylans tested. The highest activity was observed for oat spelt xylan; it was three times the activity of birchwood xylan for the crude enzyme and more than six times for the purified enzyme. The specific activity of the xylanase towards birchwood xylan was lower than oat spelt xylan and avicell. The purified xylanase did not act towards carboxymethyl cellulose compared with the crude one.
{"title":"Synergistic production and purification of extreme xylanase produced by Aspergillus flavus AUMC 10331 and A. oryzae AUMC 10329 from rice husk in solid-state fermentation","authors":"A. H. Moubasher, M. Ismail, R. A. Mohamed, Osama Abdel-Hafeez, Mohamed Al-Bedak","doi":"10.33888/jms.2019.115","DOIUrl":"https://doi.org/10.33888/jms.2019.115","url":null,"abstract":"Aspergillus flavus AUMC 10331 and A. oryzae AUMC 10329 were used in consortium culture for the production of xylanase enzyme from rice husk using solid-state fermentation technique. The xylanase purification was performed using ion exchange resin IR-120 EP and Sephadex G-75. The purified xylanase showed a total activity of 293.0 IU and a specific activity of 350.96 IU/mg protein and the enzyme was purified to 8.1 fold with 2.7% recovery. The purified xylanase was active over a wide spectrum of pHs from 3-10 and the highest activity was obtained at pH 7.0 followed by 74% of xylanase activity at pH 9.5. At pH 9.5, the xylanase exhibited its optimal activity at 70 °C indicating that the xylanase was alkaliphilic and thermophilic xylanase. The xylanase activity was greatly increased by FeSO4 and CuSO4 up to 332.15% and 194.1% respectively and slightly inhibited by CoCl2. Km and Vmax for the purified xylanase were determined at pH 9.5 and 70 °C for birchwood xylan as 22.13 mg/ml and 135.13 IU/min respectively. The crude and purified enzyme showed high specificity towards the xylans tested. The highest activity was observed for oat spelt xylan; it was three times the activity of birchwood xylan for the crude enzyme and more than six times for the purified enzyme. The specific activity of the xylanase towards birchwood xylan was lower than oat spelt xylan and avicell. The purified xylanase did not act towards carboxymethyl cellulose compared with the crude one.","PeriodicalId":48551,"journal":{"name":"Journal of Multidisciplinary Sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89522416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md. Zaherul Islam, M. A. Hakim, M. Hossain, Md. Siddikun Nabi Mandal, M. Mokhlesur, Rahman
Eleven advanced lines of spring wheat along with check varieties BARI Gom 21, BARI Gom 26 and BARI Gom 32 were evaluated at Bangladesh Wheat and Maize Research Institute (BWMRI), Dinajpur; Regional Wheat Research Center (RWRC) Gazipur; Regional Agricultural Research Station (RARS), Bangladesh Agricultural Research Institute (BARI), Jashore; RWRC, Rajshahi and RARS, BARI, Jamalpur in randomized complete block design (RCBD) with three replications during, November, 2017 to March, 2018. The trial was conducted under irrigated timely sown (ITS) and irrigated late sown (ILS) conditions. The genotypes were evaluated for yield and yield components, heading, maturity, disease reaction, etc. The effect of sowing time, location and genotype and their different interaction levels were significant for most of the traits. Wheat yield was significantly reduced in ILS condition as compared to the ITS condition. Regarding location, Dinajpur was better place for wheat production than other tested places. The mean value of the thousand-grain weight (TGW) of all genotypes was higher as compared to all check varieties. Out of eleven genotypes, ten produced a higher yield than BARI Gom 26, five produced a higher yield than BARI Gom 32. All the eleven genotypes were out yielded of the check variety BARI Gom 21. The highest yield (4099kg ha-1) was obtained in Dinajpur and the lowest yield (3108 kg ha-1) was obtained in Jamalpur. Genotypes, BAW 1290, BAW 1293, BAW 1317, BAW 1318 showed comparatively better performance in ILS condition representing that these advanced wheat lines were less affected by terminal heat stress under late sowing condition.
{"title":"Performance of some advanced spring wheat lines under optimum and late sown conditions in different regions of Bangladesh","authors":"Md. Zaherul Islam, M. A. Hakim, M. Hossain, Md. Siddikun Nabi Mandal, M. Mokhlesur, Rahman","doi":"10.33888/jms.2019.116","DOIUrl":"https://doi.org/10.33888/jms.2019.116","url":null,"abstract":"Eleven advanced lines of spring wheat along with check varieties BARI Gom 21, BARI Gom 26 and BARI Gom 32 were evaluated at Bangladesh Wheat and Maize Research Institute (BWMRI), Dinajpur; Regional Wheat Research Center (RWRC) Gazipur; Regional Agricultural Research Station (RARS), Bangladesh Agricultural Research Institute (BARI), Jashore; RWRC, Rajshahi and RARS, BARI, Jamalpur in randomized complete block design (RCBD) with three replications during, November, 2017 to March, 2018. The trial was conducted under irrigated timely sown (ITS) and irrigated late sown (ILS) conditions. The genotypes were evaluated for yield and yield components, heading, maturity, disease reaction, etc. The effect of sowing time, location and genotype and their different interaction levels were significant for most of the traits. Wheat yield was significantly reduced in ILS condition as compared to the ITS condition. Regarding location, Dinajpur was better place for wheat production than other tested places. The mean value of the thousand-grain weight (TGW) of all genotypes was higher as compared to all check varieties. Out of eleven genotypes, ten produced a higher yield than BARI Gom 26, five produced a higher yield than BARI Gom 32. All the eleven genotypes were out yielded of the check variety BARI Gom 21. The highest yield (4099kg ha-1) was obtained in Dinajpur and the lowest yield (3108 kg ha-1) was obtained in Jamalpur. Genotypes, BAW 1290, BAW 1293, BAW 1317, BAW 1318 showed comparatively better performance in ILS condition representing that these advanced wheat lines were less affected by terminal heat stress under late sowing condition.","PeriodicalId":48551,"journal":{"name":"Journal of Multidisciplinary Sciences","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84132843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S M Nadim Uddin, S. M. A. Sharif, M. H. Murad, Afrin Akter Bithy, M. M. Bhuiyan, Riaz, Hasnat Sagar
In this paper, a novel priority-based framework for semi-autonomous multi agent-based search and rescue mechanism is proposed. The framework proposes a novel multi-layered architecture consisting of multi-agents capable of target detection, role allocation, and connectivity based on access to resources. Four different operations have been proposed namely mapping, searching, coordination and information fusion. Three different roles for the agents have been proposed namely mapper, searcher, and coordinator where mapper agent contributes in developing local maps, searcher agent explores the local map for target detection and coordinator agent is responsible for cluster coordination and communication among inter-cluster, intra-cluster and information fusion module. A novel concept of cell priority index (CPI) has been proposed to determine the level of damages and zone prioritization for a rescue operation. Two different novel indices namely role suitability index and role weighting index have been proposed for efficient allocation of roles among agents. The ad-hoc network model of the proposed system is evaluated for performance metrics under different mobility mechanisms in different propagation environments to suggest possible mobility approaches to be adopted for effective search and rescue mechanisms.
{"title":"MASF: A novel priority based multi-agent framework for search and rescue","authors":"S M Nadim Uddin, S. M. A. Sharif, M. H. Murad, Afrin Akter Bithy, M. M. Bhuiyan, Riaz, Hasnat Sagar","doi":"10.33888/jms.2019.118","DOIUrl":"https://doi.org/10.33888/jms.2019.118","url":null,"abstract":"In this paper, a novel priority-based framework for semi-autonomous multi agent-based search and rescue mechanism is proposed. The framework proposes a novel multi-layered architecture consisting of multi-agents capable of target detection, role allocation, and connectivity based on access to resources. Four different operations have been proposed namely mapping, searching, coordination and information fusion. Three different roles for the agents have been proposed namely mapper, searcher, and coordinator where mapper agent contributes in developing local maps, searcher agent explores the local map for target detection and coordinator agent is responsible for cluster coordination and communication among inter-cluster, intra-cluster and information fusion module. A novel concept of cell priority index (CPI) has been proposed to determine the level of damages and zone prioritization for a rescue operation. Two different novel indices namely role suitability index and role weighting index have been proposed for efficient allocation of roles among agents. The ad-hoc network model of the proposed system is evaluated for performance metrics under different mobility mechanisms in different propagation environments to suggest possible mobility approaches to be adopted for effective search and rescue mechanisms.","PeriodicalId":48551,"journal":{"name":"Journal of Multidisciplinary Sciences","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85470678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nahid Hasan, M. M. Bhuiyan, Riaz Hasnat Sagar, S. M. Sharif
Height measurement considered as an important parameter for health monitoring. The increasing demand for telemedicine services requires a robust and affordable height measurement system. In addition, the system should have the ability to interact with a remote destination. Unfortunately, existing height measurement tools are incompatible with telemedicine services. In this paper, a low-cost autonomous height measurement system has been proposed. Moreover, the system utilizes an ultrasonic sensor to measure the human height and processes the sensor’s data through a Micro Controller Unit (MCU). Finally, the MCU communicates with the remote terminal through a Bluetooth module. A special wearable prototype has been developed to deploy the hardware for real-life applications. The experiment results demonstrate that the results obtained by the proposed system are reliable with a mean error rate of 0.02%.
{"title":"Autonomous height measurement system for smart health monitoring","authors":"Nahid Hasan, M. M. Bhuiyan, Riaz Hasnat Sagar, S. M. Sharif","doi":"10.33888/jms.2019.117","DOIUrl":"https://doi.org/10.33888/jms.2019.117","url":null,"abstract":"Height measurement considered as an important parameter for health monitoring. The increasing demand for telemedicine services requires a robust and affordable height measurement system. In addition, the system should have the ability to interact with a remote destination. Unfortunately, existing height measurement tools are incompatible with telemedicine services. In this paper, a low-cost autonomous height measurement system has been proposed. Moreover, the system utilizes an ultrasonic sensor to measure the human height and processes the sensor’s data through a Micro Controller Unit (MCU). Finally, the MCU communicates with the remote terminal through a Bluetooth module. A special wearable prototype has been developed to deploy the hardware for real-life applications. The experiment results demonstrate that the results obtained by the proposed system are reliable with a mean error rate of 0.02%.","PeriodicalId":48551,"journal":{"name":"Journal of Multidisciplinary Sciences","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82316650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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