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Changes in the Expression Landscape of HER2-Negative Breast Cancer under the Influence of Anthracycline-Containing Neoadjuvant Chemotherapy Regimens
IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-02-03 DOI: 10.1134/S1990750824600559
M. K. Ibragimova, M. M. Tsyganov, E. A. Kravtsova, N. V. Litviakov

Currently, several treatment options are available for patients with HER2 breast cancer; however, there is no gold standard first-line treatment. Anthracycline-containing regimens are also considered traditional systemic treatment as preoperative chemotherapy for this category of patients, and they are a controversial decision due to the high degree of toxicity and low complete pathological response rates. That is why new studies are needed to identify biomarkers that predict response to anthracyclines in NAC HER2-BC, both the presence of pCR and a favorable long-term outcome, to personalize preoperative treatment. In this work, changes were studied in the expression profile of breast tumor of the luminal B HER2-negative subtype under the influence of anthracycline-containing NAC regimens in order to identify the groups of potential expression markers: an objective response to the treatment used and predicting the occurrence of hematogenous metastasis.

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引用次数: 0
Novel Synthetic Kisspeptin Derivatives Impair Wound Healing in Breast Adenocarcinoma
IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-02-03 DOI: 10.1134/S1990750824600560
P. M. Kopeikin, E. G. Bogomolova, A. O. Drobintseva, T. S. Kleimenova

Currently, cancer plays an important role in public health. Mortality from cancer in 90% of cases is caused by the development of metastases, which are the clinical result of the invasiveness of the pathology. Among the known metastasis suppressor molecules are the products of the KISS1 gene—the metastin protein and its derivatives: kisspeptin-14 (KP14), kisspeptin-13 (KP13) and kisspeptin-10 (KP10). The KISS1/KISS1R system regulates the development and progression of several types of cancer. These molecules are important in the development of new treatment methods and/or as markers in the treatment of cancer, so the biologically active kisspeptin analogues design and synthesis is a promising direction for the development of drugs that prevent the metastases formation. In this study, promising sequences of kisspeptin and its modified analogues—KP-10, KP-10-dAla, KP-6-dAla—were designed. Peptides with a purity of >98% were obtained through chemical synthesis and chromatographic purification. Study of its biological activity on the MCF-7 cell line (breast adenocarcinoma) demonstrated the anti-migration activity of the peptides. The greatest effect was observed with the treatment of 1000 nM KP-10, 10 nM KP-6-dAla, 10 nM KP-10-dAla. It was shown that replacing glycine with D-alanine and shortening the kisspeptin chain led to a slower rate of wound healing of MCF-7 cells compared to control. The potential effectiveness of KP-6-dAla and KP-10-dAla in suppressing metastasis was revealed, thereby making it a promising candidate for the development of anticancer drugs.

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引用次数: 0
Improving the Functional and Antibacterial Properties of an Endoprosthetic Mesh Using Atomic Layer Deposition Technology
IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-02-03 DOI: 10.1134/S199075082470001X
R. M. Ragimov, N. M. Abdullaeva, V. Yu. Khanaliev, M. A. Khamidov, I. M. Abdulagatov

Over the past few decades, abdominal wall hernias of various locations have attracted close attention from surgeons in various countries, including Russians. Approximately 20 million hernioplasty operations are performed annually, at least 85% of which involve the use of surgical meshes. This interest of specialists in hernias is due to serious reasons, first of all, strangulation, which is a life-threatening complication and often leads to death. According to various authors, from 3 to 8% of the population suffer from hernias, and at least 20 million people in the world undergo hernia repair every day, and they are recently increasingly using endoprosthetic meshes from various manufacturers for hernial orifice repair. Often, hernia mesh causes postoperative complications, such as adhesions, wrinkling of the mesh, granulomas, fistulas, seromas, including infection of the implant, leading to the development of abscesses and possibly sepsis. To reduce these complications, various modifications of endoprosthetic meshes and materials are being developed. However, there is not a single mesh at present that would completely satisfy hernia surgeons. Based on the above, the authors set a goal to improve the functional and antibacterial properties of polypropylene endoprosthetic mesh using atomic layer deposition (ALD) nanotechnology. The ALD method consists in that a nanofilm is created on the surface of the endoprosthetic mesh through cyclic reactions using trimethylaluminum, titanium tetrachloride, and vanadium trichloroxide. The authors have obtained the first samples of meshes that were implanted into the bodies of experimental animals. The experimental samples demonstrated good biocompatibility and integration into the surrounding tissue, preventing any inflammatory response. The developed method for improving the functional and antibacterial properties of endoprosthetic meshes is simple in industrial implementation compared to PACVD or CVD and allows covering large areas, therefore, reducing material production costs and increasing the economic efficiency of domestically produced mesh implants and making them competitive in the world market.

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引用次数: 0
The Role of Arachidonic Acid Metabolizing Cytochromes P450 in the Control of Cardiovascular Functions 花生四烯酸代谢细胞色素 P450 在控制心血管功能中的作用
IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-05 DOI: 10.1134/S1990750824600158
M. L. Perepechaeva, A. Yu. Grishanova

Arachidonic acid (AA) is widely distributed in many organs and can be metabolized in several ways into small lipid molecules: eicosanoides that perform a physiological and/or pathophysiological role. Among them, epoxyeicosatrienoic acids (EET) and hydroxyeicosatetraenoic acids (HETE), which are produced by cytochrome P450 (CYP) enzymes, have attracted much attention. AA metabolites play an important role in such cellular processes as proliferation, differentiation, and apoptosis and are also involved in the development of organs and tissues as well as in the pathogenetic processes underlying the development of various diseases; in particular, AA metabolites formed with the participation of CYP are involved in normal and pathological processes associated with the functioning of the cardiovascular system. This review focuses on the formation and cardiovascular effects of CYP-generated AA metabolites and summarizes current literature data on the physiological and pathophysiological significance of the CYP-dependent AA metabolic pathway and its relevance to the cardiovascular system in both health and disease.

花生四烯酸(AA)广泛分布于许多器官,可通过多种方式代谢成小脂质分子:二十烷酸,发挥生理和/或病理生理作用。其中,由细胞色素 P450(CYP)酶产生的环氧二十碳三烯酸(EET)和羟基二十碳四烯酸(HETE)备受关注。AA 代谢物在细胞增殖、分化和凋亡等过程中发挥重要作用,还参与器官和组织的发育以及各种疾病的发病过程;特别是,在 CYP 参与下形成的 AA 代谢物参与了与心血管系统功能相关的正常和病理过程。本综述侧重于 CYP 生成的 AA 代谢物的形成及其对心血管的影响,并总结了有关依赖 CYP 的 AA 代谢途径的生理和病理生理学意义及其与心血管系统在健康和疾病中的相关性的现有文献数据。
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引用次数: 0
Effect of Elevated Concentrations of Antibodies on the Immune Response in Patients with Autoimmune Thyroiditis 抗体浓度升高对自身免疫性甲状腺炎患者免疫反应的影响
IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-05 DOI: 10.1134/S1990750823600565
K. V. Vereshchagina, A. V. Samodova, L. K. Dobrodeeva

A study is performed of the effect elevated concentrations of antibodies have on the immune response in patients with autoimmune thyroiditis (AIT) living in Arkhangelsk. Results from immunological examinations of 108 individuals with AIT are presented. The immunological study included examining hemograms, the contents of phenotypes of lymphocytes, cytokines, immunoglobulins, and antibodies of thyroid peroxidase (TPO), double-stranded DNA, and nucleoproteins. It was estanlished that a rise in the concentration of antibodies (AB) to TPO, ds-DNA, to RNP in patients with AIT is associated with elevated contents of mature T lymphocytes (CD3+), cytotoxic T lymphocytes (CD8+), cells labeled for programmed cell death (CD95+), and a drop in concentrations of IL-10. An increase in the concentration of cytotoxic lymphocytes and cells labeled for apoptosis and a simultaneous drop in the concentration of regulatory IL-10 is not beneficial in terms of prognostic value for the accumulation of autoantigens and autoantibodies.

对居住在阿尔汉格尔斯克的自身免疫性甲状腺炎(AIT)患者体内抗体浓度升高对免疫反应的影响进行了研究。本文介绍了对108名自身免疫性甲状腺炎患者进行免疫学检查的结果。免疫学研究包括检查血液图、淋巴细胞表型、细胞因子、免疫球蛋白、甲状腺过氧化物酶(TPO)抗体、双链DNA和核蛋白的含量。研究发现,AIT 患者体内 TPO、ds-DNA 和 RNP 抗体 (AB) 浓度的升高与成熟 T 淋巴细胞(CD3+)、细胞毒性 T 淋巴细胞(CD8+)、程序性细胞死亡标记细胞(CD95+)含量的升高以及 IL-10 浓度的下降有关。细胞毒性淋巴细胞和标记细胞凋亡的细胞浓度增加,同时调节性 IL-10 浓度下降,这对自身抗原和自身抗体积累的预后价值并无益处。
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引用次数: 0
Expression of miR-21, miR-378a, miR-205, and Their Targets in ER-Positive Breast Tumors with Different HER2 Protein Levels miR-21、miR-378a、miR-205 及其靶标在不同 HER2 蛋白水平的 ER 阳性乳腺肿瘤中的表达
IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-05 DOI: 10.1134/S1990750824600195
G. R. Abdullin, T. S. Kalinina, V. V. Kononchuk, D. A. Obukhova, I. S. Valembakhov, D. D. Zakharova, S. I. Makarova, L. F. Gulyaeva

Tyrosine protein kinase HER2 plays an important role in carcinogenesis. In breast cancer (BC), the HER2 gene is amplified in approximately 20% of cases. Trastuzumab is used to treat BC with HER2 gene amplification. Trastuzumab is not effective in treating tumors with low HER2 expression, but trastuzumab deruxtecan was recently found to significantly improve prognosis in these patients. Nonetheless, there are still difficulties with accurate diagnosis of HER2-low BC, especially at the preoperative stage. In this study, when comparing the results between core needle biopsies and resection specimens using three immunohistochemistry scores (0, 1+, and 3+) of the HER2 protein level, we observed only moderate agreement (66.2%, κ = 0.486) in patients who did not undergo neoadjuvant therapy (n = 71). Other miRNA- or protein-coding genes regulated by HER2 signaling pathways may be additional markers for the scoring of the HER2 level. We measured levels of HER2-regulated miR-378a, -205, and -21 and mRNA levels of their target genes TRPS1, ITGA2, BCL6, and PTEN in BC surgical specimens. For estrogen receptor-positive BC (n = 64), we confirmed that the expression of miR-21, miR-378a, TRPS1, PTEN, and BCL6 is associated with the HER2 protein level. Moreover, the expression profile of miR-378a, BCL6, and PTEN differed between HER2 1+ and HER2 3+ tumors. TRPS1 expression was significantly higher in HER2 3+ tumors compared with HER2 0 tumors, but there was no difference in the amount of TRPS1 mRNA between HER2 1+ and HER2 3+ tumors. Thus, an analysis of the expression of miR-21, miR-378a, TRPS1, PTEN, and BCL6 may help to distinguish between HER2-negative, HER2-positive, and HER2-low BCs.

酪氨酸蛋白激酶 HER2 在致癌过程中发挥着重要作用。在乳腺癌(BC)中,HER2 基因扩增的病例约占 20%。曲妥珠单抗用于治疗 HER2 基因扩增的乳腺癌。曲妥珠单抗对治疗 HER2 表达较低的肿瘤无效,但最近发现曲妥珠单抗德鲁司康能显著改善这些患者的预后。尽管如此,HER2 低表达 BC 的准确诊断仍然存在困难,尤其是在术前阶段。在本研究中,当使用三种免疫组化评分(0、1+ 和 3+)比较核心针活检和切除标本的 HER2 蛋白水平时,我们发现在未接受新辅助治疗的患者(n = 71)中,两者的一致性仅为中等(66.2%,κ = 0.486)。其他受 HER2 信号通路调控的 miRNA 或蛋白编码基因可能是 HER2 水平评分的额外标记。我们测量了BC手术标本中受HER2调控的miR-378a、-205和-21的水平及其靶基因TRPS1、ITGA2、BCL6和PTEN的mRNA水平。对于雌激素受体阳性的 BC(n = 64),我们证实 miR-21、miR-378a、TRPS1、PTEN 和 BCL6 的表达与 HER2 蛋白水平相关。此外,miR-378a、BCL6和PTEN的表达情况在HER2 1+和HER2 3+肿瘤中有所不同。TRPS1 在 HER2 3+ 肿瘤中的表达明显高于 HER2 0 肿瘤,但 TRPS1 mRNA 的数量在 HER2 1+ 和 HER2 3+ 肿瘤中没有差异。因此,分析 miR-21、miR-378a、TRPS1、PTEN 和 BCL6 的表达有助于区分 HER2 阴性、HER2 阳性和 HER2 低的 BCs。
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引用次数: 0
Effects of Various Poly(A) Tails on Luciferase Expression 各种聚(A)尾对荧光素酶表达的影响
IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-05 DOI: 10.1134/S1990750824600055
I. Kukushkin, O. Vasileva, D. Kunyk, S. Kolmykov, T. Sokolova, A. Muslimov, I. Terenin, O. Zaborova, A. Kirshina, F. Kolpakov, R. Ivanov, V. Reshetnikov

Synthesis of RNA by in vitro transcription has many applications in the production of vaccines, anticancer and gene therapy medicines. Despite several advantages of mRNA molecules over DNA in terms of transient transgene expression, rapid degradation of RNA is the limiting factor for the efficacy of mRNA-based therapeutics. Translational efficiency and stability of mRNA depends on the sequence of 5′ and 3′ untranslated regions, cap structure, coding sequence (CDS), and the poly(A) tail. So far, the role of the poly(A) tail sequence in mRNA stability and translational efficiency remains poorly investigated. There are few studies evaluating the influence of poly(A) tails containing certain non-A nucleotides or segments of non-A nucleotides on the efficacy of mRNA therapeutics. In our work, we conducted a comprehensive assessment of the effects of mRNA poly(A) tail on luciferase expression in cell lines HEK293 and DC2.4 and in BALB/c mice. Our results showed that mRNAs with a poly(A) tail consisting of 100 adenines separated by a segment of 10 nucleotides (50A-GCAUAUGACU-50A) have the best translational efficiency both in vitro (in cell lines) and in vivo (in BALB/c mice). Nonetheless, the results were inconsistent and depended both on cell lines and on the coding sequence (NanoLuc or firefly luciferase). Thus, effects of a mRNA poly(A) tail on luciferase expression may depend on the sequence of the gene in question and on specific features of the expression of RNA-binding proteins in cell lines.

通过体外转录合成 RNA 在生产疫苗、抗癌药物和基因治疗药物方面有许多应用。尽管与 DNA 相比,mRNA 分子在瞬时转基因表达方面具有一些优势,但 RNA 的快速降解是限制基于 mRNA 的疗法疗效的因素。mRNA 的翻译效率和稳定性取决于 5′和 3′非翻译区的序列、帽结构、编码序列(CDS)和多聚(A)尾。迄今为止,关于多聚(A)尾序列在 mRNA 稳定性和翻译效率中的作用的研究仍然很少。很少有研究评估含有某些非 A 核苷酸或非 A 核苷酸片段的聚(A)尾对 mRNA 治疗效果的影响。在我们的工作中,我们全面评估了 mRNA 多(A)尾对 HEK293 和 DC2.4 细胞系以及 BALB/c 小鼠荧光素酶表达的影响。我们的结果表明,mRNA 的聚(A)尾由 100 个腺嘌呤组成,腺嘌呤之间相隔 10 个核苷酸(50A-GCAUAUGACU-50A),在体外(细胞系)和体内(BALB/c 小鼠)的翻译效率最高。然而,结果并不一致,而且取决于细胞系和编码序列(NanoLuc 或萤火虫荧光素酶)。因此,mRNA 聚(A)尾对荧光素酶表达的影响可能取决于相关基因的序列以及细胞系中 RNA 结合蛋白表达的具体特征。
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引用次数: 0
Contribution of Matrix Metalloproteinases in the Pathogenesis of Cardiovascular Diseases Complicated by Obesity 基质金属蛋白酶在肥胖并发心血管疾病发病机制中的作用
IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-05 DOI: 10.1134/S1990750823600504
S. R. Ledovskikh, Ya. V. Polonskaya, V. S. Shramko

Cardiovascular diseases (CVDs) are one of the world’s main causes of mortality. The role of matrix metalloproteinases (MMPs) as markers of CVD complications has been actively discussed in recent years. It has been proven that a high level of MMPs contributes to the development of unstable plaque, aggravating a patient’s prognosis. Being an independent risk factor, obesity also results in complications of cardiovascular diseases. Studies on the effect an increase in the body mass index and adipose tissue hormones on the level of MMPs have begun to appear. Results from clinical and experimental studies over the last 5 years devoted to studying matrix metalloproteinases are presented here. The role of MMPs in the pathogenesis of atherosclerosis is reflected. MMPs are considered biomarkers of the development of adverse cardiovascular events. Results from studies on the effect obesity has on the level of MMPs are also presented here. A search was performed for articles in the PubMed, Google Scholar, and Elibrary databases for the period from 2018 to 2023 using the keywords metalloproteinases and atherosclerosis, metalloproteinases and obesity, and the connection between atherosclerosis and obesity separately for each type of MMP.

心血管疾病(CVDs)是导致全球死亡的主要原因之一。近年来,基质金属蛋白酶(MMPs)作为心血管疾病并发症标志物的作用得到了积极的讨论。事实证明,高水平的 MMPs 会导致不稳定斑块的形成,从而加重患者的预后。作为一个独立的风险因素,肥胖也会导致心血管疾病的并发症。有关身体质量指数和脂肪组织激素的增加对 MMPs 水平影响的研究已经开始出现。本文介绍了过去 5 年中专门研究基质金属蛋白酶的临床和实验研究结果。MMPs在动脉粥样硬化发病机制中的作用得到了反映。基质金属蛋白酶被认为是心血管不良事件发生的生物标志物。本文还介绍了有关肥胖对 MMPs 水平影响的研究结果。以金属蛋白酶与动脉粥样硬化、金属蛋白酶与肥胖、动脉粥样硬化与肥胖之间的联系为关键词,分别针对每种类型的MMP在PubMed、Google Scholar和Elibrary数据库中检索了2018年至2023年期间的文章。
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引用次数: 0
The Concentration of Telomeric Repeat Binding Factor 2 (TERF2) in Leukocyte Cells of Patients with Hepatitis 肝炎患者白细胞中端粒重复结合因子 2 (TERF2) 的浓度
IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-05 DOI: 10.1134/S1990750824600092
Endang Purwaningsih, Tripanjiasih Susmiarsih, Yenni Zulhamidah, Achmad Sofwan

Hepatitis is a disease that attacks the organs of the human liver that causes inflammation that makes the liver function. Telomere length and telomerase activity in chronic liver disease and hepatocarcinoma correlate with disease severity. Telomeres are protected by her six shelterin proteins or telosome, namely TERF1/TRF1, TERF2/TRF2, POT1, TPP1, RAP1, and TIN2. TRF2 protein has an important role in regulating the molecular events that maintain telomere integrity. The objective of the research was to determine the concentration of TERF2 in leukocytes in patients with hepatitis. The research design method is descriptive with a case-control study involving 62 subjects. Blood samples were taken from patients with hepatitis from Klinik Pratama Prof. Qomariyah, which amounted to 32 people, including 8 males and 24 females (40–60 years). As a control, employees of YARSI University with the same age range, amounting to 24 people, including 20 men and 4 women. Measurements of TERF2 used the ELISA method. TERF2 protein concentrations in patients with hepatitis leukocytes were significantly higher (p < 0.05) compared to controls. Similarly, TERF2 levels between male patients with male controls and between female patients with female controls also showed significant differences in sufficient concentration (p < 0.05). Instead, the concentrations of TERF2 between patients with hepatitis men and women did not have a difference. The concentration of TERF2 in leucocyte patients with hepatitis showed a concentration higher than in healthy people, both men and women.

肝炎是一种侵犯人体肝脏器官的疾病,会引起炎症,从而影响肝脏功能。慢性肝病和肝癌的端粒长度和端粒酶活性与疾病的严重程度相关。端粒由六种保护蛋白或端粒体保护,即TERF1/TRF1、TERF2/TRF2、POT1、TPP1、RAP1和TIN2。TRF2蛋白在调节维持端粒完整性的分子事件中发挥着重要作用。研究的目的是确定肝炎患者白细胞中 TERF2 的浓度。研究设计方法为描述性病例对照研究,涉及 62 名受试者。血液样本取自 Klinik Pratama Prof. Qomariyah 的肝炎患者,共 32 人,包括 8 名男性和 24 名女性(40-60 岁)。作为对照的是雅西大学的员工,他们的年龄范围相同,共 24 人,包括 20 名男性和 4 名女性。采用酶联免疫吸附法测定 TERF2。与对照组相比,肝炎患者白细胞中的 TERF2 蛋白浓度明显更高(p <0.05)。同样,男性患者与男性对照组之间以及女性患者与女性对照组之间的 TERF2 含量也有显著差异(p <0.05)。相反,男女肝炎患者之间的 TERF2 浓度没有差异。无论男女,肝炎患者白细胞中的 TERF2 浓度均高于健康人。
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引用次数: 0
The Effects of Pretreatment with Atorvastatin, Fenofibrate, or Both Drugs in a Mouse Model of Acute Lipemia Induced by the General Lipase Inhibitor Poloxamer 407 阿托伐他汀、非诺贝特或两种药物的预处理对普通脂肪酶抑制剂聚氧乙烯-407 诱导的急性脂肪血症小鼠模型的影响
IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-05 DOI: 10.1134/S1990750823600474
T. A. Korolenko, T. P. Johnston, N. V. Tamkovich, V. A. Vavilin, N. P. Bgatova, I. D. Ivanov, G. S. Russkikh, E. V. Koldysheva, E. C. Korolenko, V. I. Kapustina, S. I. Makarova, N. V. Goncharova, M. M. Gevorgyan, V. M. Loginova

Dyslipidemia is a well-known risk factor for the development of cardiovascular diseases and atherosclerosis. The effects of combined pretreatment with atorvastatin and fenofibrate (Tricor) were studied in a mouse model of acute lipemia induced by a general lipase inhibitor, poloxamer 407 (P-407, 250 mg/kg). This lipemia is characterized by significantly increased serum levels of triglycerides (TG), low-density lipoprotein (LDL) cholesterol, together with decreased concentration of high-density lipoprotein (HDL) cholesterol. Atorvastatin pretreatment had a hypolipidemic effect, decreasing concentrations of LDL cholesterol and increasing HDL cholesterol. Pretreatment of mice with fenofibrate decreased TG level, increasing HDL cholesterol. Combined pretreatment with atorvastatin and fenofibrate decreased TG and total cholesterol. Elevation of the serum cystatin C level was found in control and lipemic mice pretreated with atorvastatin, fenofibrate, or both. Liver expression of lysosomal acid lipase increased in atorvastatin- or/and fenofibrate-pretreated groups of lipemic mice. It was concluded that increased expression of lysosomal acid lipase is related to the removal of lipid droplets from hepatocytes, thus preventing acute lipemia. Lastly, cystatin C may be a “theranostic” biomarker for hypolipidemic drugs.

众所周知,血脂异常是导致心血管疾病和动脉粥样硬化的危险因素。研究人员在一种由普通脂肪酶抑制剂多聚酶 407(P-407,250 毫克/千克)诱发的急性脂血症小鼠模型中研究了阿托伐他汀和非诺贝特(Tricor)联合预处理的效果。这种脂血症的特点是血清中甘油三酯(TG)和低密度脂蛋白(LDL)胆固醇水平明显升高,同时高密度脂蛋白(HDL)胆固醇浓度降低。阿托伐他汀预处理具有降血脂作用,可降低低密度脂蛋白胆固醇的浓度,提高高密度脂蛋白胆固醇的浓度。对小鼠进行非诺贝特预处理可降低总胆固醇水平,增加高密度脂蛋白胆固醇。阿托伐他汀和非诺贝特联合预处理可降低总胆固醇和总胆固醇。在对照组和使用阿托伐他汀、非诺贝特或两者进行预处理的高脂血症小鼠中发现血清胱抑素 C 水平升高。阿托伐他汀或/和非诺贝特预处理组的高脂血症小鼠肝脏溶酶体酸性脂肪酶表达增加。结论是溶酶体酸性脂肪酶表达的增加与清除肝细胞中的脂滴有关,从而防止了急性脂血症的发生。最后,胱抑素 C 可能是降血脂药物的 "治疗 "生物标志物。
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引用次数: 0
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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry
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